CN104206172A - Cultivation method for selenium-enriched oyster mushrooms with small mushroom caps and large stipes - Google Patents

Cultivation method for selenium-enriched oyster mushrooms with small mushroom caps and large stipes Download PDF

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Publication number
CN104206172A
CN104206172A CN201410430837.XA CN201410430837A CN104206172A CN 104206172 A CN104206172 A CN 104206172A CN 201410430837 A CN201410430837 A CN 201410430837A CN 104206172 A CN104206172 A CN 104206172A
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China
Prior art keywords
selenium
oyster mushrooms
enriched
cultivation
mushroom
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CN201410430837.XA
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Chinese (zh)
Inventor
刘安军
李兵芳
张志刚
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SHANXI ZITUAN ECOLOGICAL AGRICULTURE Co Ltd
Tianjin University of Science and Technology
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SHANXI ZITUAN ECOLOGICAL AGRICULTURE Co Ltd
Tianjin University of Science and Technology
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Priority to CN201410430837.XA priority Critical patent/CN104206172A/en
Publication of CN104206172A publication Critical patent/CN104206172A/en
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Abstract

The invention relates to a cultivation method for selenium-enriched oyster mushrooms with small mushroom caps and large stipes. The cultivation method comprises the following steps: preparing a cultivation bag; sterilizing; cooling; inoculating; managing a greenhouse, and the like. The total selenium amount of selenium-enriched cultivation materials is 10-50mg/kg and selenic acid arginine is used as a selenium source; main materials and auxiliary materials adopted by the selenium-enriched cultivation materials are as follows in dry parts by weight: 45% of corncobs, 45% of cottonseed hulls, 9% of bran, 1% of corn meal and 0.05%-0.1% of lime. The selenic acid arginine is used as the selenium source so that the toxin is small and the safety is high; the absorption utilization rate of the selenium is high. The selenium content of sporocarps of the obtained selenium-enriched oyster mushrooms is high, the residual amount of the inorganic selenium is less and the eating safety is high; the disadvantage that the selenium content of the oyster mushrooms is lower is made up and the nutritional health value of the oyster mushrooms is improved. The sporocarps of the selenium-enriched oyster mushrooms have the small mushroom caps and the large stipes, the mouth feel and the appearance quality of the selenium-enriched oyster mushrooms are greatly enhanced and the commodity value of the oyster mushrooms is improved.

Description

The breeding method of the large stem mushroom with abundant selenium of a kind of pileolus
Technical field
The present invention relates to the method for the selenium nutrition-intensified and quality improving of a kind of edible mushroom, particularly a kind of is that selenium source carries out breeding method that is selenium nutrition-intensified and the large stem mushroom with abundant selenium of pileolus with Selenoarginine.
Background technology
Selenium is one of trace element of needed by human, is the activated centre of the multiple enzyme such as glutathione peroxidase, de-iodine enzyme, thioredoxin reductase, has weight meaning to maintenance human normal physiological function.Research finds, selenium have anti-oxidant, improve erythrocytic oxygen carrying capacity, improve human immunologic function, protect cardiovascular and myocardium health, cancer-resisting, affect human thyroglobulin metabolism, the different physiological roles such as the natural antipoison of many kinds of metal ions.
Selenium is at occurring in nature skewness, and content is rare.From the Three Eastern Provinces to the Yunnan-Guizhou Plateau, there is the low selenium area of Tiao Zhan China area 72% in China, wherein the area of 30% seriously lacks selenium, and about 700,000,000 populations are in scarce selenium state.Therefore, in meals, increase the absorption of selenium, exploitation selenium condensed food, especially develops safety, efficiently Organic Selenium condensed food, has become the important content of China's health food development.
Flat mushroom strong adaptability, growth cycle are short, biological transformation ratio is high, are world's second largest edible fungus culturing kinds, have important economy, ecology and medical value.Mushroom carpophore meat is smooth fresh and tender, delicious flavour, is rich in protein, amino acid, raw fiber and various mineral matter, and be the desirable food materials of high protein and low fat, consumer groups are extensive.With the carrier that flat mushroom is selenium nutrition-intensified, significant to the healthy nutritive value improving flat mushroom.
At present, often utilize the inorganic seleniums such as sodium selenite to cultivate mushroom with abundant selenium for selenium source, toxicity is comparatively large, threaten practitioner healthy, selenium absorption rate is low, there is pollution hidden trouble; The mushroom with abundant selenium inorganic selenium residual quantity of gained is high, and edible safety is poor.Organic selenium source that toxicity is little carrys out Enriching Selenium as albumen selenium, yeast selenium etc. are often sprayed at crop surface, and this method cost is high, rich selenium weak effect, does not utilize spread.
Summary of the invention
The invention provides a kind of breeding method of high-quality mushroom with abundant selenium.
The technical solution adopted in the present invention is:
(1) cultivation bag is prepared: corncob is after the Se content prepared by Selenoarginine is soak 10 ~ 16h in the rich selenium liquid of 25 ~ 100mg/kg, mix with other main ingredients in the planting materials such as cotton seed hulls, wheat bran, corn flour, lime, controlling planting material water content is 54% ~ 58%, Se content is 10 ~ 50mg/kg, abundant stirring and evenly mixing, load in plastic sack, after perforating, add ring sealing.
(2) sterilizing: cultivation bag is put in sterilizing cabin, and temperature arrives 100 DEG C, steam sterilizing 12 ~ 14h, is incubated 10h after stopping heating.
(3) cool: sterilizing completes, and opens hatch door, after temperature is down to 80 DEG C, pushes cooling chamber forced refrigeration, until temperature is down to 30 DEG C.
(4) inoculate: single-termination kind under employing aseptic condition, inoculum concentration is 4% ~ 6%.
(5) booth management: issue bacterium the natural conditions of 10 ~ 25 DEG C.
As optimization, described rich selenium liquid prepares gained by Selenoarginine: taking parts by weight is that the Selenoarginine of 8 ~ 30 parts joins in the aqueous solution of 100000 parts, and mix, Se content is 25 ~ 100mg/kg.
As optimization, described selenium-enriching cultivation material water content is 54% ~ 58%, and Se content is 10 ~ 50 mg/kg.
As optimization, the percentage of the main ingredient dry weight of described selenium-enriching cultivation material is: corncob: 45%;
Cotton seed hulls: 45%;
Wheat bran: 9%;
Corn flour: 1%;
Lime: 0.05% ~ 0.1%.
As optimization, described selenium-rich corn core in rich selenium liquid, soaks 12 ~ 16h by the corncob pulverized to form.
As optimization, the feature of described mushroom with abundant selenium is that bacteria cover diameter is less than common flat mushroom by 10% ~ 30%, and stem grows 10% ~ 20% than common flat mushroom, stem thicker than common flat mushroom 10% ~ 30%.
Advantage of the present invention and good effect are:
The present invention take Selenoarginine as selenium source, and toxicity is little, safety is high, and selenium absorption rate is high.Gained mushroom with abundant selenium fruit body Se content of the present invention is high, and inorganic selenium less residue, edible safety are high, compensate for the shortcoming that flat mushroom Se content is lower, improve the healthy nutritive value of flat mushroom.Gained mushroom with abundant selenium fruit body of the present invention, the little stem of cap is large, greatly strengthen mouthfeel and the exterior quality of mushroom with abundant selenium, improves the commodity value of flat mushroom.
Embodiment
Below by specific embodiment, the invention will be further described, and following examples are descriptive, is not determinate, can not limit protection scope of the present invention with this.
Embodiment 1
(1) cultivation bag is prepared: be, after soaking 12h in the rich selenium liquid of 50mg/kg, mix with other main ingredients in planting material at the Se content prepared by Selenoarginine by the corncob after pulverizing.The percentage of the main ingredient dry weight of planting material is:
Corncob: 45%;
Cotton seed hulls: 45%;
Wheat bran: 9%;
Corn flour: 1%;
Lime: 0.05%.
Regulate planting material water content to be 55%, Se content is 25mg/kg, abundant stirring and evenly mixing, loads in plastic sack, adds ring sealing after perforating.
(2) sterilizing: cultivation bag is put in sterilizing cabin, and temperature arrives 100 DEG C, steam sterilizing 12 ~ 14h, is incubated 10h after stopping heating.
(3) cool: sterilizing completes, and opens hatch door, after temperature is down to 80 DEG C, pushes cooling chamber forced refrigeration, until temperature is down to 30 DEG C.
(4) inoculate: single-termination kind under employing aseptic condition, inoculum concentration is 5%.
(5) booth management: issue bacterium the natural conditions of 10 ~ 25 DEG C.
The bacteria cover diameter of the mushroom with abundant selenium of gained reduces 15%, and stem length adds 10%, and stem diameter adds 12%, and fruit body Se content reaches 34mg/kg(dry weight).
Embodiment 2
(1) cultivation bag is prepared: be, after soaking 12h in the rich selenium liquid of 100mg/kg, mix with other main ingredients in planting material at the Se content prepared by Selenoarginine by the corncob after pulverizing.The percentage of the main ingredient dry weight of planting material is:
Corncob: 45%;
Cotton seed hulls: 45%;
Wheat bran: 9%;
Corn flour: 1%;
Lime: 0.05%.
Regulate planting material water content to be 58%, Se content is 50mg/kg, abundant stirring and evenly mixing, loads in plastic sack, adds ring sealing after perforating.
(2) sterilizing: cultivation bag is put in sterilizing cabin, and temperature arrives 100 DEG C, steam sterilizing 12 ~ 14h, is incubated 10h after stopping heating.
(3) cool: sterilizing completes, and opens hatch door, after temperature is down to 80 DEG C, pushes cooling chamber forced refrigeration, until temperature is down to 30 DEG C.
(4) inoculate: single-termination kind under employing aseptic condition, inoculum concentration is 5%.
(5) booth management: issue bacterium the natural conditions of 10 ~ 25 DEG C.
The bacteria cover diameter of the mushroom with abundant selenium of gained reduces 28%, and stem length adds 15%, and stem diameter adds 12%, and fruit body Se content reaches 77mg/kg(dry weight).

Claims (6)

1. a breeding method for the large stem mushroom with abundant selenium of pileolus, is characterized in that comprising the following steps:
(1) prepare cultivation bag: get corncob and join in rich selenium liquid and soak, obtain selenium-rich corn core, then mix with other main ingredients in planting material, abundant stirring and evenly mixing, obtain selenium-enriching cultivation material, finally load in plastic sack, after perforating, add ring sealing;
(2) sterilizing: cultivation bag is put in sterilizing cabin, and temperature arrives 100 DEG C, steam sterilizing 12 ~ 14h, is incubated 10h after stopping heating;
(3) cool: sterilizing completes, and opens hatch door, after temperature is down to 80 DEG C, pushes cooling chamber forced refrigeration, until temperature is down to 30 DEG C;
(4) inoculate: single-termination kind under employing aseptic condition, inoculum concentration is 4% ~ 6%;
(5) booth management: issue bacterium the natural conditions of 10 ~ 25 DEG C.
2. method according to claim 1, is characterized in that described rich selenium liquid prepares gained by Selenoarginine: taking parts by weight is that the Selenoarginine of 8 ~ 30 parts joins in the aqueous solution of 100000 parts, and mix, Se content is 25 ~ 100mg/kg.
3. method according to claim 1, it is characterized in that described selenium-enriching cultivation material water content is 54% ~ 58%, Se content is 10 ~ 50 mg/kg.
4. method according to claim 1, is characterized in that the percentage of the main ingredient dry weight of described selenium-enriching cultivation material is: corncob: 45%;
Cotton seed hulls: 45%;
Wheat bran: 9%;
Corn flour: 1%;
Lime: 0.05% ~ 0.1%.
5. method according to claim 1, is characterized in that described selenium-rich corn core, is in rich selenium liquid, to soak 12 ~ 16h by the corncob pulverized to form.
6. method according to claim 1, is characterized in that the feature of described mushroom with abundant selenium is that bacteria cover diameter is less than common flat mushroom by 10% ~ 30%, and stem grows 10% ~ 20% than common flat mushroom, stem thicker than common flat mushroom 10% ~ 30%.
CN201410430837.XA 2014-08-29 2014-08-29 Cultivation method for selenium-enriched oyster mushrooms with small mushroom caps and large stipes Pending CN104206172A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104584864A (en) * 2015-02-03 2015-05-06 何寒 Cultivating method for selenium-enriched oyster mushrooms
CN104641936A (en) * 2015-01-29 2015-05-27 谢光玉 Cultivation method for selenium-enriched oyster mushrooms
CN104756752A (en) * 2015-01-21 2015-07-08 常德市湘西北仿野生富硒食用菌农民专业合作社 Method for planting selenium-rich edible mushrooms by using whole crop straws
CN111052978A (en) * 2018-10-16 2020-04-24 郭立潮 Astragalus membranaceus mushroom cultivation method

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CN101755615A (en) * 2009-12-31 2010-06-30 张寿禄 Cultivation method of high trace element edible and medical fungi
CN102150555A (en) * 2010-12-09 2011-08-17 苏州硒谷科技有限公司 Method for culturing pleurotus geesteranus uniformly rich in selenium by using mixed selenium source
CN102617240A (en) * 2012-04-10 2012-08-01 绥化学院 Novel edible fungus culture medium and preparation method thereof
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104756752A (en) * 2015-01-21 2015-07-08 常德市湘西北仿野生富硒食用菌农民专业合作社 Method for planting selenium-rich edible mushrooms by using whole crop straws
CN104641936A (en) * 2015-01-29 2015-05-27 谢光玉 Cultivation method for selenium-enriched oyster mushrooms
CN104584864A (en) * 2015-02-03 2015-05-06 何寒 Cultivating method for selenium-enriched oyster mushrooms
CN111052978A (en) * 2018-10-16 2020-04-24 郭立潮 Astragalus membranaceus mushroom cultivation method

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