CN105638240A - Selenium-and-calcium-rich high-yield oyster mushroom cultivation method - Google Patents

Selenium-and-calcium-rich high-yield oyster mushroom cultivation method Download PDF

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Publication number
CN105638240A
CN105638240A CN201511033821.6A CN201511033821A CN105638240A CN 105638240 A CN105638240 A CN 105638240A CN 201511033821 A CN201511033821 A CN 201511033821A CN 105638240 A CN105638240 A CN 105638240A
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selenium
culture medium
parts
calcium
bed
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刘大卫
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JIESHOU JUFENG HOUSEHOLD FARM
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JIESHOU JUFENG HOUSEHOLD FARM
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/50Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

A selenium-and-calcium-rich high-yield oyster mushroom cultivation method comprises culture medium preparation, strain sowing and later-period management. A culture medium is prepared from, by weight, 30-60 parts of folium mori, 45-60 parts of mature cereal straw, 50-70 parts of dry cow dung and 0.5-1 part of straw fermentation agent, and the culture medium is obtained after smashing and evenly mixing the raw materials, adding water for multiple times of fermentation, and then conducting airing. The culture medium is laid into a culture bed at the thickness of 8-12 cm, strain is evenly spread on the surface of the bed, and the surface of the bed is then covered with a layer of oyster mushroom culture medium; environment temperature is controlled to be 20-26 DEG C, and relative air humidity is kept to be 70-80%; after strain is sown, hyphae start to grow, most hyphae extend to the bottom of the mushroom bed within 10-15 days, and the mushroom bed is then covered with the oyster mushroom culture medium which is 3-5 cm thick; later-period management is conducted in the conventional way till maturity and harvesting. Oyster mushroom cultivated with the method is not limited by territory or environment and is rich in selenium and high in yield, and the method can serve as a selenium supplement approach easy to popularize in a selenium-efficient area.

Description

A kind of high yield mushroom cultivation method of selenium-rich and calcium
Technical field
The present invention relates to technical field of crop cultivation, specifically a kind of high yield mushroom cultivation method of selenium-rich and calcium.
Background technology
Pleurotus ostreatus (formal name used at school: Pleurotusostreatus; Have another name called pick up the ears, oyster cap fungus, oyster mushroom, black Paeonia suffruticosa mushroom, Taiwan is also known as Pleurotus geesteranus) be Agaricales Pleurotaceae one kind under Basidiomycota, be a kind fairly common Lycoperdon polymorphum Vitt edible mushroom.
Pleurotus ostreatus is containing abundant nutrient substance, and every hectogram is in product containing 20 23 grams of protein, and aminoacid ingredient A wide selection of colours and designs, content of mineral substances is very abundant.
The traditional Chinese medical science thinks that Pleurotus ostreatus is warm in nature, sweet in the mouth. There is effect of wind dispelling cold expelling, relaxing muscles and tendons and activating QI and blood in the collateral. For controlling the diseases such as lumbago and skelalgia, numb hand and foot, grain be obstructed. Cancerous cell is had very strong inhibitory action by the proteoglycan body in Pleurotus ostreatus, can enhancing human body immunity function.
Selenium is the trace element of needed by human. Chinese Soclety of Nutrition is one of 15 kinds of nutrients being classified as needed by human by selenium also, a large amount of clinical experiments both at home and abroad are bright, human body lacks selenium can cause the functional disorder of some vitals, cause that many serious diseases occur, more than 40, whole world country is in scarce selenium area, several hundred million populations in 22 provinces of China are all in scarce selenium or low selenium area, and the sickness rate such as the population tumor in these areas, hepatopathy, cardiovascular disease is significantly high.
Some areas of 12 provinces and cities of middle state-two, about 700,000,000 people live in low selenium area. Most area Selenium In Foods content is at below 0.02mg/kg, and southeastern coast is China's district rich in selenium, also only has 0.10mg/kg, is the minimum that specifies of the United Nations's health organization. Chinese population food does not still reach the standard selenium supplement level of more than everyone 40 micrograms every day, especially lacks selenium area crowd, it more difficult to from food, reach the purpose of selenium supplement.
Inorganic selenium and sodium selenite, started to disable in the world from 2000. Yeast selenium, Fructus Hordei Germinatus selenium, nanometer selenium (beer yeast+sodium selenite) absorption of human body and metabolic rate are low.Human absorptivity is 40%-60% only, and remains remaining as inorganic selenium.
What enjoy favor is natural organic plant activity selenium, such as 100 microgram vegetable active selenium (maize rich in selenium powder), the pure grain product of selenium element are absorbed from the soil of technique of enriched selenium improvement, photosynthesis in growth course and vivo biodistribution transformation, exist with seleno-amino acids form in vivo, human absorptivity reaches more than 99%, has both met human body selenium element needs, solves again absorption and the metabolic rate difficult problem on the low side of selenium.
Calcium is biological required element. For human body, no matter in muscle, nerve, body fluid and skeleton, all useful Ca2+In conjunction with protein. Calcium is the main inorganic composition of mankind's bone, tooth, is also the necessary elements such as neurotransmission, muscle contraction, blood clotting, hormone release and galactopoiesis. Calcium accounts for the 1.4% of body mass, participates in metabolism, and every day must be supplemented with calcium; In human body, calcium content deficiency or surplus all can affect growth promoter and health.
Calcium is the inorganic salt component that in human body, content is maximum, and in health adult's body, calcium total amount is about 1,000��1,300 grams, about the 1.5%��2.0% of percentage of liveweight. Wherein the calcium of 99% is present in bone mineral form in skeleton and tooth, and all the other are distributed in soft tissue, and the calcium in extracellular fluid only accounts for the 0.1% of TC. Bone is the main portions of doped calcium, so there being the title in " calcium storehouse ". Bone calcium mainly exists with two kinds of forms of hydroxyapatite of non-crystal calcium hydrogen phosphate and crystal, and its composition and materialization property constantly change with Human Physiology or pathologic condition. In area of new bone, calcium hydrogen phosphate is more than outmoded bone, is transformed into hydroxyapatite gradually in skeletal maturation process. Skeleton makes bone calcium keep dynamic equilibrium with blood calcium by continuous skeletonization and osteolysis.
Summary of the invention
The weak point that the present invention exists in order to avoid prior art, a kind of high yield mushroom cultivation method of selenium-rich and calcium is provided, the Pleurotus ostreatus of the inventive method cultivation is not by region and environmental limitation, cultivate the Pleurotus ostreatus not only Se content of acquisition, calcium content is abundant but also yield is high, scarce selenium area can be become the approach of a kind of easily universal selenium supplement is provided, calcium can also be supplemented when selenium supplement simultaneously.
This invention address that technical problem adopts the following technical scheme that
A kind of high yield mushroom cultivation method of selenium-rich and calcium, it is characterized in that, comprises the steps,
1) with parts by weight, take calcite in powder 5-10 part, ripe Semen Sesami straw 65-80 part, Folium Mori 30-60 part, calcium nitrate 1-3 part, dry cattle manure 50-70 part, stover ferment agent 0.5-1 part, mixing after above-mentioned raw materials is pulverized, obtain batch mixing, it is subsequently adding 30-50 part water and drenches batch mixing, room temperature airtight condition bottom fermentation one week, stirring after fermenting one week, add the water of 15-20 part, continue fermentation two weeks, again after stirring, the nutritional solution being eventually adding 10-15 part continues fermentation 3-5 days, after fermentation ends, add people's quick lime, the pH value of fermented product is adjusted to 7.0, steam sterilization 8-10h, then fermented product is dried, obtain oyster mushroom culture medium, in every liter of water in described fermented nutritive liquid, phosphoric acid potassium dihydrogen 15-18g, magnesium sulfate 6-12g, four water manganese sulfate 8-12g, ferrous sulfate heptahydrate 25-30g, sodium selenite 8-10g,
2) by the thickness of 8-12cm, oyster mushroom culture medium being laid to bed, strain is sprinkling upon a surface uniformly, then covers lid layer by oyster mushroom culture medium on bed surface, thickness is 1-2cm, spreads water and makes surface moisture, then gently presses, and makes culture medium and strain be fully contacted; Ambient temperature controls at 20-26 DEG C, and relative air humidity is maintained at 70%-80%;
3) after strain is sowed, mycelia starts growth, and in 10-15 days, mycelia major part has been elongated to bottom mushroom bed, then is covered with the thick oyster mushroom culture medium of 3-5cm; Follow-up by common field management.
Feature of the present invention also resides in:
Described Folium Mori are fresh selenium-rich Folium Mori, and full Se content fresh weight is at more than 4mg/kg.
Described step 1) in, with parts by weight, calcite in powder 10 parts, ripe Semen Sesami straw 75 parts, 50 parts of Folium Mori, calcium nitrate 3 parts, dry cattle manure 70 parts.
Described stover ferment agent active component includes the raw material of following weight percents: bacillus subtilis 25%, saccharomyces cerevisiae 18%, aspergillus niger 12%, streptomyces hygroscopicus 10%, lactic acid bacteria 15%, enterococcus faecalis 7%; Healthy and free from worry Trichoderma spp. 13%.
In every liter of water in described fermented nutritive liquid, phosphoric acid potassium dihydrogen 18g, magnesium sulfate 7g, four water-calcium nitrate 95g, four water manganese sulfate 10g, ferrous sulfate heptahydrate 26g, sodium selenite 10g.
Compared with the prior art, the present invention has the beneficial effect that:
Mushroom cultivation method of the present invention is not by region and environmental limitation, and the Pleurotus ostreatus not only Se content that cultivation obtains is abundant but also yield is high, it is possible to becoming scarce selenium area provides the approach of a kind of easily universal selenium supplement, can also supplement calcium when selenium supplement simultaneously.
Below by way of detailed description of the invention, technical solution of the present invention is further explained explanation.
Detailed description of the invention
A kind of selenium-rich high yield mushroom cultivation method, comprises the steps,
1) with parts by weight, take calcite in powder 5-10 part, ripe Semen Sesami straw 65-80 part, Folium Mori 30-60 part, calcium nitrate 1-3 part, dry cattle manure 50-70 part, stover ferment agent 0.5-1 part, mixing after above-mentioned raw materials is pulverized, obtain batch mixing, it is subsequently adding 30-50 part water and drenches batch mixing, room temperature airtight condition bottom fermentation one week, stirring after fermenting one week, add the water of 15-20 part, continue fermentation two weeks, again after stirring, the nutritional solution being eventually adding 10-15 part continues fermentation 3-5 days, after fermentation ends, add people's quick lime, the pH value of fermented product is adjusted to 7.0, steam sterilization 8-10h, then fermented product is dried, obtain oyster mushroom culture medium, in every liter of water in described fermented nutritive liquid, phosphoric acid potassium dihydrogen 15-18g, magnesium sulfate 6-12g, four water manganese sulfate 8-12g, ferrous sulfate heptahydrate 25-30g, sodium selenite 8-10g,
2) by the thickness of 8-12cm, oyster mushroom culture medium being laid to bed, strain is sprinkling upon a surface uniformly, then covers lid layer by oyster mushroom culture medium on bed surface, thickness is 1-2cm, spreads water and makes surface moisture, then gently presses, and makes culture medium and strain be fully contacted; Ambient temperature controls at 20-26 DEG C, and relative air humidity is maintained at 70%-80%;
3) after strain is sowed, mycelia starts growth, and in 10-15 days, mycelia major part has been elongated to bottom mushroom bed, then is covered with the thick oyster mushroom culture medium of 3-5cm; Follow-up carry out Routine Management by prior art.
When being embodied as, Folium Mori are fresh selenium-rich Folium Mori, and full Se content fresh weight, at more than 4mg/kg, can select from district rich in selenium or the selenium-rich Folium Mori obtained through selenium-enriching cultivation.
As preferably, with parts by weight, 50 parts of Folium Mori, ripe grain straw 45 parts, dry cattle manure 60 parts, wherein ripe grain straw includes Barley straw, rice straw, corn straw, Semen setariae straw etc.; It is namely broken that dry cattle manure is that cattle manure dry in the sun under field conditions (factors) to hands is pinched.
Stover ferment agent preferred version in the present embodiment is: raw material by weight percentage is: bacillus subtilis 25%, saccharomyces cerevisiae 18%, aspergillus niger 12%, streptomyces hygroscopicus 10%, lactic acid bacteria 15%, enterococcus faecalis 7%;Healthy and free from worry Trichoderma spp. 13%.
In the present embodiment, fermented nutritive liquid is preferably: in every liter of water, phosphoric acid potassium dihydrogen 18g, magnesium sulfate 7g, four water-calcium nitrate 95g, four water manganese sulfate 10g, ferrous sulfate heptahydrate 26g, sodium selenite 10g.
Detect with the Pleurotus ostreatus selenium of preferred version cultivation acquisition, calcium content with the present invention, existing atomic fluorescence spectrometry is adopted to detect, linear relationship according to absorption spectrum with selenium, calcium content, obtain the selenium in measuring samples, calcium content, Pleurotus ostreatus full Se content (wet basis) the average out to 3.725 �� g/g that this cultural method obtains after testing, calcium content (wet basis) is 2.45mg/g. About 40 days fruitings of cultural method of the present invention simultaneously, the Pleurotus ostreatus piece type gone out is good, meat is plump, without dead mushroom, sick mushroom, average bacteria cover diameter can reach 11.4cm, 3-4 tide of can gathering, head tide average organism conversion ratio 67%, second tide average organism conversion ratio is 43%, and the average conversion of the 3rd tide and the 4th tide is 24%.

Claims (4)

1. the high yield mushroom cultivation method of a selenium-rich and calcium, it is characterised in that comprise the steps,
1) with parts by weight, take calcite in powder 5-10 part, ripe Semen Sesami straw 65-80 part, Folium Mori 30-60 part, calcium nitrate 1-3 part, dry cattle manure 50-70 part, stover ferment agent 0.5-1 part, mixing after above-mentioned raw materials is pulverized, obtain batch mixing, it is subsequently adding 30-50 part water and drenches batch mixing, room temperature airtight condition bottom fermentation one week, stirring after fermenting one week, add the water of 15-20 part, continue fermentation two weeks, again after stirring, the nutritional solution being eventually adding 10-15 part continues fermentation 3-5 days, after fermentation ends, add people's quick lime, the pH value of fermented product is adjusted to 7.0, steam sterilization 8-10h, then fermented product is dried, obtain oyster mushroom culture medium, in every liter of water in described fermented nutritive liquid, phosphoric acid potassium dihydrogen 15-18g, magnesium sulfate 6-12g, four water manganese sulfate 8-12g, ferrous sulfate heptahydrate 25-30g, sodium selenite 8-10g,
2) by the thickness of 8-12cm, oyster mushroom culture medium being laid to bed, strain is sprinkling upon a surface uniformly, then covers lid layer by oyster mushroom culture medium on bed surface, thickness is 1-2cm, spreads water and makes surface moisture, then gently presses, and makes culture medium and strain be fully contacted; Ambient temperature controls at 20-26 DEG C, and relative air humidity is maintained at 70%-80%;
3) after strain is sowed, mycelia starts growth, and in 10-15 days, mycelia major part has been elongated to bottom mushroom bed, then is covered with the thick oyster mushroom culture medium of 3-5cm; Follow-up by common mode management to ripe harvesting.
2. a kind of selenium-rich high yield mushroom cultivation method according to claim 1, it is characterised in that described step 1) in, with parts by weight, calcite in powder 10 parts, ripe Semen Sesami straw 75 parts, 50 parts of Folium Mori, calcium nitrate 3 parts, dry cattle manure 70 parts.
3. the high yield mushroom cultivation method of a kind of selenium-rich according to claim 1 and calcium, it is characterized in that, it is characterized in that, described stover ferment agent active component includes the raw material of following weight percents: bacillus subtilis 25%, saccharomyces cerevisiae 18%, aspergillus niger 12%, streptomyces hygroscopicus 10%, lactic acid bacteria 15%, enterococcus faecalis 7%; Healthy and free from worry Trichoderma spp. 13%.
4. the high yield mushroom cultivation method of a kind of selenium-rich according to claim 1 or 3 and calcium, it is characterized in that, in every liter of water in described fermented nutritive liquid, phosphoric acid potassium dihydrogen 18g, magnesium sulfate 7g, four water manganese sulfate 10g, ferrous sulfate heptahydrate 26g, sodium selenite 10g.
CN201511033821.6A 2015-12-31 2015-12-31 Selenium-and-calcium-rich high-yield oyster mushroom cultivation method Pending CN105638240A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107950291A (en) * 2017-12-28 2018-04-24 山东常生源菌业有限公司 A kind of needle mushroom production method
CN108076968A (en) * 2017-12-28 2018-05-29 山东常生源菌业有限公司 A kind of culture medium for golden mushroom
CN108432549A (en) * 2018-05-08 2018-08-24 铜仁科学院 The culturing raw material and method of the low accumulation heavy metal elegant precious mushroom high-yield culturing of selenium-rich
CN109042074A (en) * 2018-08-28 2018-12-21 铜陵盛牛菌业有限责任公司 A kind of oyster mushroom culture medium and preparation method thereof
CN111183851A (en) * 2020-03-14 2020-05-22 成都师范学院 Cultivation method of high-calcium edible fungi
CN112521192A (en) * 2020-11-26 2021-03-19 百色学院 Preparation method of novel selenium-rich bionic cultivation nutrient solution

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CN101857489A (en) * 2010-05-14 2010-10-13 东至县德丰食用菌科技开发中心 Zinc-enriched selenium-enriched lentinus edodes and cultivation method thereof
CN102517217A (en) * 2011-12-27 2012-06-27 何颖霞 Environment-friendly straw fermenting agent

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1370396A (en) * 2002-04-09 2002-09-25 梁峥 Selenium-rich mushroom and its cultivation process
CN1437841A (en) * 2002-12-27 2003-08-27 贾磊钦 Culturing method for biological selenium products
CN101602621A (en) * 2009-06-25 2009-12-16 山东农业大学 Production system of selenium-enriched product based on recycling ecological agriculture
CN101857489A (en) * 2010-05-14 2010-10-13 东至县德丰食用菌科技开发中心 Zinc-enriched selenium-enriched lentinus edodes and cultivation method thereof
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107950291A (en) * 2017-12-28 2018-04-24 山东常生源菌业有限公司 A kind of needle mushroom production method
CN108076968A (en) * 2017-12-28 2018-05-29 山东常生源菌业有限公司 A kind of culture medium for golden mushroom
CN108432549A (en) * 2018-05-08 2018-08-24 铜仁科学院 The culturing raw material and method of the low accumulation heavy metal elegant precious mushroom high-yield culturing of selenium-rich
CN109042074A (en) * 2018-08-28 2018-12-21 铜陵盛牛菌业有限责任公司 A kind of oyster mushroom culture medium and preparation method thereof
CN111183851A (en) * 2020-03-14 2020-05-22 成都师范学院 Cultivation method of high-calcium edible fungi
CN112521192A (en) * 2020-11-26 2021-03-19 百色学院 Preparation method of novel selenium-rich bionic cultivation nutrient solution

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