CN102150558A - Method for culturing pleurotus geesteranus uniformly rich in selenium by utilizing sodium selenate - Google Patents

Method for culturing pleurotus geesteranus uniformly rich in selenium by utilizing sodium selenate Download PDF

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CN102150558A
CN102150558A CN 201010580174 CN201010580174A CN102150558A CN 102150558 A CN102150558 A CN 102150558A CN 201010580174 CN201010580174 CN 201010580174 CN 201010580174 A CN201010580174 A CN 201010580174A CN 102150558 A CN102150558 A CN 102150558A
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selenium
cultivation
particle
additive
mushroom
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CN102150558B (en
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李飞
李红丽
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SUZHOU SE TECHNOLOGY Co Ltd
Suzhou Setek Co Ltd
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SUZHOU SE TECHNOLOGY Co Ltd
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Abstract

The invention discloses a method for culturing pleurotus geesteranus uniformly rich in selenium by utilizing sodium selenate. The method comprises the following steps: mixing the selenium-enriched cultivation material additive with the dry cultivation material, wherein the weight of the selenium-enriched cultivation material additive is 1-10% of the dry cultivation material, and then culturing according to a common culture method, thereby acquiring the pleurotus geesteranus uniformly rich in selenium, wherein the total selenium content in the selenium-enriched cultivation material additive is 5-500mg/kg; the sizes of the grains in the selenium-enriched cultivation material additive are 0.005-5.0mm and the additive is in various grain sizes area distribution; the grains, the sizes of which are 1.0-5.0mm are at least 10% of the weight of the additive; the grains, the sizes of which are 0.005-0.1mm are at least 40% of the weight of the additive; and the selenium-enriched cultivation material additive comprises the following raw materials in parts by weight: 100 weight parts of corncob powder and 0.0012-0.12 weight parts of sodium selenate. The selenium is high in use ratio, the selenium contents in the pleurotus geesteranus in different batches are stable, the method is free from hidden danger of selenium pollution, the operation is convenient, and the method is easily popularized.

Description

Utilize sodium selenate to carry out the method for the precious mushroom of even selenium-enriching cultivation show
Technical field
The invention provides a kind of is that selenium source carries out the selenium nutrition-intensified method of edible mushroom with the sodium selenate, especially relates to the selenium nutrition-intensified method of the precious mushroom of a kind of show, belongs to the conversion technology field of selenium resource.
Background technology
Elegant precious mushroom (Pleurotus geesteranus) belongs to Eumycota, Basidiomycetes, Agaricales, Pleurotaceae Pleurotus on taxonomy.Elegant precious mushroom quality is tender and crisp, fresh and sweet tasty and refreshing, and delicious flavour is nutritious, has higher edible and medical value, is the strain excellent in the oyster cap fungus.It is a kind of high protein, low-fat nutraceutical, and its protein content approaches meat, than the high 3-6 of general vegetables doubly, and its quality delicacy, fiber content is few; More valuablely be, it contains ethnic group self and can not make, and the threonine that lacks usually in the food, lysine, homo amino acid etc.This kind edible mushroom shape is small and exquisite, and is delicious in taste, very popular, and in very great demand in the international market, DEVELOPMENT PROSPECT is wide.
Selenium is a kind of human body trace elements necessary, has anticancer, anti-oxidant (aging) and improves biological propertyes such as body immunity, antagonism heavy metal.It is the 50-250 microgram that daily intaking amount is recommended by Chinese Soclety of Nutrition.But China adult's day selenium intake only has 26-50 μ g, and therefore, the selemium nutrition condition of poor is at China's ubiquity.There are 72% low selenium in county (city) or scarce selenium in China, and Heilungkiang, the Inner Mongol, Gansu, Qinghai, Sichuan etc. seriously lack the selenium area has Keshan disease, Kaschin-Beck disease to take place, and certain cancers district occurred frequently (as the Jiangsu Qidong City) also belongs to low selenium district.The data that WHO announces shows that the whole world has more than 40 country to belong to low selenium or scarce selenium area.Rich selenium agricultural byproducts are human body Selenium Supplement element effective ways, and therefore, technique of enriched selenium is scientists study hot of research and development field.Scarce selenium can bring out angiocardiopathy, tumour, Keshan disease etc.It is too much to take the photograph the selenium amount, and selenosis takes place easily.The nutritionist points out that except that dietary fiber, edible mushroom contains the complete nutrients matter of needed by human body, and an adult eats the 100g edible mushroom every day, can obtain the 15-17g high-quality protein; Can obtain 600mg calcium, be equivalent to 80% of daily requirement; Can satisfy 11% of human body amphemera requirement, but Se content is lower than 5 μ g/kg in the general edible mushroom.
At present more to the rich selenium report of various edible mushrooms: the soil earthing that Chinese invention patent " a kind of cultural method that is rich in the selenium mushroom " (patent No.: ZL 02116566.1) adopts rich selenium natural material ramulus mori and is rich in selenium is cultivated mushroom, and to obtain selenium content be the Agaricus campestris rich in selenium of 0.3mg/kg; " rich zinc, the rare Edible Fungi method of selenium " (patent No.: CN00113180.X) provide a kind of rare parent edible fungus kind, original seed, production kind to make and the method for cultivating, promptly by in bacterium culture mediums at different levels, add a certain amount of by the sodium selenite solution preparation contain the selenium humus soil, in cultivate material, add the method for sodium selenite solution and produce a kind of rare edible mushroom of being rich in selenium; " selenium-enriched edible mushroom cultivation method " (patent No.: ZL 200610046148.4) adopts and add selenium salt then sprays selenium-rich nutritive liquid on the bacterium rod method cultivation of selenium-rich edible mushroom earlier in culturing raw material; Chinese invention patent " preparation method of selenium enriched oyster mushroom powder " (publication number: CN1951221A) disclose a kind of method of liquid culture selenium enriched oyster mushroom silk.
And the subject matter that prior art exists has: the elegant precious mushroom Se content that the traditional planting technology mode is produced is low, and added value is low, can not become the Dietotherapy health product; The rich selenium mushroom that the method for employing Spraying Se-fertilizer obtains is inorganic residual too many, and safety is relatively poor; The rich selenium mushroom of natural selenium-rich manufacture of materials is adopted in indivedual areas, and the method practicality is limited by the region, promotes difficulty; Adopt and earlier bacterial classifications at different levels to be tamed the method for again edible mushroom being carried out selenium-enriching cultivation step by step, process is loaded down with trivial details, complicated operation and be subjected to technical limitations, and generally planting the family, to implement resistance big; Common selenium-enriching cultivation method: the selenium salting liquid added to cultivate in the medium to obtaining selenium-enriched edible mushroom, be difficult to realize controllability in the whole incubation to the product selenium content; The rich selenium amount fluctuation of fruit body big and selenium form safety and less stable etc.
At present the report that the precious mushroom of show is carried out rich selenium research seldom directly add sodium selenite solution and the selenium-enriching cultivations of other edible mushrooms also mostly is in cultivate material, and major part rests on laboratory or small test stage.
Summary of the invention
Problems such as the object of the invention is to provide the even selenium-enriching cultivation method of the precious mushroom of a kind of show, and it is batch inhomogeneous to have solved in the prior art behind the selenium-enriching cultivation between the growth of elegant precious mushroom head tide or many tide growth elegant each strains of precious mushroom in back Se content, and the rich selenium amount fluctuation of fruit body is big.
In order to solve these problems of the prior art, technical scheme provided by the invention is:
A kind of method of utilizing sodium selenate to carry out the precious mushroom of even selenium-enriching cultivation show, it is characterized in that described method is included in cultivation and sneaks into 1~10% the selenium-enriching cultivation feed additives that accounts for cultivation siccative gross weight, the step of cultivating the elegant precious mushroom that obtains even rich selenium according to the common cultivation method in the siccative; Wherein to contain the selenium total amount be 5~500mg/kg to the selenium-enriching cultivation feed additives, and grain diameter is many particle size region and distributes in the selenium-enriching cultivation feed additives in 0.005~5.0mm scope, and particle diameter is that the particle of 1.0~5.0mm accounts for 10% of additive gross weight at least; Particle diameter is that the particle of 0.005~0.1mm accounts for 40% of additive gross weight at least; The component raw material that described selenium-enriching cultivation feed additives adopts comprises in parts by weight separately:
Maize cob meal 100 parts by weight;
Sodium selenate 0.0012~0.12 parts by weight.
Preferably, grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005~5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10~20% of 1.0~5.0mm;
Particle diameter is the particle 40~70% of 0.005~0.1mm;
The particle 20~40% of other particle size range.
Preferably, grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005~5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10% of 1.0~5.0mm;
Particle diameter is the particle 50% of 0.005~0.1mm;
The particle 40% of other particle size range.
Preferably, grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005~5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 20% of 1.0~5.0mm;
Particle diameter is the particle 50% of 0.005~0.1mm;
The particle 30% of other particle size range.
Preferably, described cultivation siccative comprises with the weight percent meter of its component:
Corncob 75%;
Cotton seed hulls 20%;
Quicklime 3%;
Land plaster 1%;
Composite fertilizer 1%.
Preferably, described common cultivation method comprises that the cultivation siccative that will sneak into the selenium-enriching cultivation feed additives adds running water according to material-water ratio, abundant stirring and evenly mixing, heap fermentation at 1: 1.3, the steam sterilization sterilization treatment is used in the pack back, makes the even selenium enriched oyster mushroom of final composts or fertilisers of cultivating inoculated and cultured.
Preferably, the maize cob meal that described selenium-enriching cultivation feed additives uses is pulverized as natural crops offcuts corncob and is formed.
Preferably, the particle of described selenium-enriching cultivation feed additives is by finishing granulation process after maize cob meal and the sodium selenate raw material pulverizing.
Preferably, during the cultivation of the medicine that is used to keep healthy food selenium enriched oyster mushroom, the Se content of the selenium-enriching cultivation feed additives of use is 200~500mg/kg; When being used for the cultivation of daily edible selenium enriched oyster mushroom, the Se content of the selenium-enriching cultivation feed additives of use is 5~100mg/kg.
Wherein A~B% represents scope in A% arrives the B% scope, and comprises B% and A%.
The inventor is through a large amount of research and test, in conjunction with elegant precious mushroom the utilize advantage and the many tides of edible mushroom of certain culturing raw material are grown and the different characteristics of each damp amount of growth, by preparing a kind of different proportioning features of different grain size that have, can discharge the specialist additive of selenium wherein with different speed, providing a kind of is that selenium source carries out the selenium nutrition-intensified method of elegant precious mushroom with the sodium selenate, realizes the stability of rich selenium amount between each damp mushroom.It is good to cultivate safety by this invention, the precious mushroom of stay-in-grade organic selenium-rich show, and then provide how safe and reliable organic selenium source for human body.The concrete steps of the technical solution used in the present invention can for:
At first prepare a kind of selenium enriched oyster mushroom cultivate material additive:
1. with a kind of natural crops offcuts corncob by screening, dry, to be crushed to particle diameter be 0.005~0.1mm, mixing, designs the prescription of the required cultivate material additive of the precious mushroom of cultivation of selenium-rich show.
This additive formulations is as follows:
100 parts of maize cob meals
0.0012~0.12 part of sodium selenate;
2. above-mentioned sodium selenate being ground to particle diameter is 0.005~0.1mm powder, with the abundant stirring and evenly mixing of maize cob meal, makes particle diameter below 0.1mm, and selenium content is elementary compound in 5~500mg/kg scope.
3. the above-mentioned compound processing of part granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.1~1.0mm, the 1.0~5.0mm scope.
4. above-mentioned three kinds of particle diameters are respectively: the particle of 0.005~0.1mm, 0.1~1.0mm, 1.0~5.0mm mixes by following percentage by weight: 40~70%, 20~40%, 10~20%, promptly obtain having the selenium-enriching cultivation feed additives of different grain size proportioning feature, its total selenium content is still in the 5-500mg/kg scope.
According to the difference of required selenium enriched oyster mushroom product purpose, select the different cultivate material additive of selenium content during use.For example, daily edible rich selenium mushroom cultivation can be selected the selenium enriched oyster mushroom cultivate material additive in 5~100mg/kg scope; The health care medicine is eaten rich selenium mushroom cultivation can select the interior cultivate material additive of 200~500mg/kg scope.
During the cultivate material spice, the ratio in 1~10% is sneaked into the common cultivation material with additive, and stirring and evenly mixing makes in the cultivate material final selenium content in 0.5~50mg/kg scope.
Press material-water ratio 1: 1.3 and add running water, fully stir, mixing once more, heap fermentation, the steam sterilization sterilization treatment is used in the pack back, makes final composts or fertilisers of cultivating inoculated and cultured selenium enriched oyster mushroom; Also can directly adopt the cultivation in raw material mode to produce the selenium enriched oyster mushroom.
When the precious massee fruiting bodies of show was gathered, sampling detected wherein Se content, and bright sample Se content improves 6~280 times than the precious mushroom of common cultivation show, can reach 0.2~10mg/kg.And the selenium that exists with the selenomethionine form in the fruit body accounts for total selenium about 66~73%.
Correct damp mushroom output is added up, and finds that selenium nutrition-intensified group is improved more than 20% than common cultivation group output.
Frying on an equal basis under the condition, allowing 5 personnel that the precious mushroom of common show and selenium enriched oyster mushroom mushroom meat mouthfeel, local flavor are assessed, the result shows: selenium enriched oyster mushroom quality is more tender and crisp, taste is more delicious.
In the whole growth of fruit body in the cycle, the relative standard deviation RSD of sample selenium content can be strict controlled in 20%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD is higher than 30%) edible mushroom of the additive cultivation of different grain size proportioning feature; Selenium content is more stable in the same stage fruit body, and RSD all is lower than 8%.
Utilize the present invention can obtain the respond well elegant precious mushroom of rich selenium, promoting elegant precious mushroom product is the selenium-enriched health care product, is that daily delicacies can be used as the dietotherapy good merchantable brand again, and the people that are more convenient for accept.
With respect to scheme of the prior art, advantage of the present invention is:
(1) the cultivate material additive for preparing among the present invention is the selenium-enriched edible mushroom specialist additive, combines the edible fungi growth characteristics, has different grain size proportioning feature, can realize that selenium wherein discharges with different speed, thereby realizes the stability of rich selenium amount between each damp mushroom.
(2) above-mentioned additive is a selenium source with the sodium selenate, is that selenium source carrier and effective selenium replenish with the maize cob meal; Maize cob meal can effectively prevent the loss of selenium source and guarantee the even distribution of selenium source, not only helped ensureing that elegant precious mushroom to the full and uniform absorption of selenium source but also can promote edible fungi growth, improves output.
(3) additive component is simple, and is easy to use.Only need during use when the cultivate material spice with the common cultivation material by a certain percentage (1~10%) mix, make the cultivate material of final selenium content in 0.5~50mg/kg scope, can guarantee that elegant precious mushroom is to the absorption safely and effectively of selenium element, enrichment and conversion.
(4) the present invention realizes the stability of additive quality by steps such as elementary compound preparation, granulation and each granularity particle mix in varing proportions; Realize mixing once more by spice before the cultivate material fermentation, this just guarantees the even distribution of selenium resource in cultivate material, is beneficial to the even absorption to selenium of mycelium and fruit body.
(5) can realize that by the present invention inorganic selenium changes into the good biogenic selenium that exists with the amino acid form in the elegant precious massee fruiting bodies in the cultivate material.Testing result shows that selenomethionine is the main occurrence patterns of selenium in the elegant precious mushroom, and this form selenium accounts for 66~73% of total Se content.
(6) use the selenium enriched oyster mushroom that the present invention produces, bright mushroom selenium content can be strict controlled between 0.2~2.5mg/kg and the 4~10mg/kg, and the former can be for daily edible, and the latter can be used as food therapeutic health.
(7) the present invention can effectively improve elegant precious mushroom output, and makes elegant precious mushroom mouthfeel better, and local flavor is brighter.
(8) to be suitable for any material be the elegant precious mushroom cultivation of cultivation base-material to the additive rich in selenium of the present invention preparation, as sawmilling end, wheat bran, corn tangerine bar, corncob, rape straw, cotton seed hulls etc.
Embodiment
Below in conjunction with specific embodiment such scheme is described further.Should be understood that these embodiment are used to the present invention is described and are not limited to limit the scope of the invention.The implementation condition that adopts among the embodiment can be done further adjustment according to the condition of concrete producer, and not marked implementation condition is generally the condition in the normal experiment.
Embodiment 1 elegant precious mushroom selenium-enriching cultivation embodiment
The preparation of selenium enriched oyster mushroom additive:
1. with a kind of crops leftovers, corncob by screening, dry, to be crushed to particle diameter be 0.005~0.1mm, the prescription of the required cultivate material additive of the precious mushroom of design cultivation of selenium-rich show.
This additive formulations is as follows:
100 parts of maize cob meals;
0.0012~0.0024 part of sodium selenate.
2. above-mentioned sodium selenate being ground to particle diameter is 0.005~0.1mm powder, with the abundant stirring and evenly mixing of maize cob meal, makes particle diameter below 0.1mm, and selenium content is elementary compound in 5~10mg/kg scope.
3. above-mentioned compound processing granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.005~0.1mm, 0.1~1.0mm, the 1.0~5.0mm scope.
4. above-mentioned three kinds of size particles are mixed by following percentage by weight: 70%, 20%, 10%, promptly obtain having different grain size proportioning feature, selenium content is the selenium-enriching cultivation feed additives of 5~10mg/kg.
(2) elegant precious mushroom is selenium nutrition-intensified:
In certain edible mushroom base, select additive for use as preparation as described in (1); The common cultivation material is formed: corncob 75%, cotton seed hulls 20%, quicklime 3%, land plaster 1%, composite fertilizer 1%.When the cultivate material spice, ratio in 10% and common cultivation material mixing, making final selenium content is 0.5~1mg/kg; Press material-water ratio 1: 1.3 and add running water, fully stir, mixing once more, heap fermentation, the steam sterilization sterilization treatment use in the pack back, makes final composts or fertilisers of cultivating inoculation, a bacterium; Cultivate according to precious mushroom cultivation of normal show and management method.
Implementation result:
Correct damp mushroom output is added up, and finds selenium nutrition-intensified group than common cultivation group output raising 20%.When each damp mushroom is ripe, pluck, carry out the mensuration of total selenium, organic selenium: total Se content is in 0.2~0.5mg/kg scope in the selenium enriched oyster massee fruiting bodies, and wherein the selenium that exists with the seleno-amino acids form accounts for about 70% of total selenium.The whole growth of fruit body is in the cycle, the relative standard deviation RSD of selenium content can be strict controlled in 20%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD is higher than 30%) elegant precious mushroom of the additive cultivation of different grain size proportioning feature; Selenium content is more stable in the same stage fruit body, and RSD all is lower than 8%.
Frying on an equal basis under the condition, allowing 5 personnel that the precious mushroom of common show and selenium enriched oyster mushroom mushroom meat mouthfeel, local flavor are assessed, the result shows: selenium enriched oyster mushroom quality is more tender and crisp, taste is more delicious.
Embodiment 2 elegant precious mushroom selenium-enriching cultivation embodiment
(1) preparation of selenium enriched oyster mushroom additive:
1. with a kind of crops leftovers, corncob by screening, dry, to be crushed to particle diameter be 0.005~0.1mm, the prescription of the required cultivate material additive of the precious mushroom of design cultivation of selenium-rich show.
This additive formulations is as follows:
100 parts of maize cob meals;
0.0024~0.012 part of sodium selenate.
2. above-mentioned sodium selenate being ground to particle diameter is 0.005~0.1mm powder, with the abundant stirring and evenly mixing of maize cob meal, makes particle diameter below 0.1mm, and selenium content is elementary compound in 10~50mg/kg scope.
3. above-mentioned compound processing granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.005~0.1mm, 0.1~1.0mm, the 1.0~5.0mm scope.
4. above-mentioned three kinds of size particles are mixed by following percentage by weight: 60%, 30%, 10%, promptly obtain having different grain size proportioning feature, selenium content is the selenium-enriching cultivation feed additives of 10~50mg/kg.
(2) elegant precious mushroom is selenium nutrition-intensified:
In certain edible mushroom base, select additive for use as preparation as described in (1); The common cultivation material is formed: corncob 75%, cotton seed hulls 20%, quicklime 3%, land plaster 1%, composite fertilizer 1%.When the cultivate material spice, ratio in 10% and common cultivation material mixing, making final selenium content is 1~5mg/kg; Press material-water ratio 1: 1.3 and add running water, fully stir, mixing once more, heap fermentation, the steam sterilization sterilization treatment use in the pack back, makes final composts or fertilisers of cultivating inoculation, a bacterium; Cultivate according to precious mushroom cultivation of normal show and management method.
Implementation result:
Correct damp mushroom output is added up, and finds selenium nutrition-intensified group than common cultivation group output raising 20%.When each damp mushroom is ripe, pluck, carry out the mensuration of total selenium, organic selenium: total Se content is in 0.5~2.5mg/kg scope in the selenium enriched oyster massee fruiting bodies, and wherein the selenium that exists with the seleno-amino acids form accounts for about 70% of total selenium.The whole growth of fruit body is in the cycle, the relative standard deviation RSD of selenium content can be strict controlled in 20%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD is higher than 30%) elegant precious mushroom of the additive cultivation of different grain size proportioning feature; Selenium content is more stable in the same stage fruit body, and RSD all is lower than 8%.
Frying on an equal basis under the condition, allowing 5 personnel that the precious mushroom of common show and selenium enriched oyster mushroom mushroom meat mouthfeel, local flavor are assessed, the result shows: selenium enriched oyster mushroom quality is more tender and crisp, taste is more delicious.
Embodiment 3 elegant precious mushroom selenium-enriching cultivation embodiment
(1) preparation of selenium enriched oyster mushroom additive:
1. with a kind of crops leftovers, corncob by screening, dry, to be crushed to particle diameter be 0.005~0.1m m, the prescription of the required cultivate material additive of the precious mushroom of design cultivation of selenium-rich show.
This additive formulations is as follows:
100 parts of maize cob meals;
0.012~0.06 part of sodium selenate.
2. above-mentioned sodium selenate being ground to particle diameter is 0.005~0.1mm powder, with the abundant stirring and evenly mixing of maize cob meal, makes particle diameter below 0.1mm, and selenium content is elementary compound in 50~100mg/kg scope.
3. above-mentioned compound processing granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.005~0.1mm, 0.1~1.0mm, the 1.0~5.0mm scope.
4. above-mentioned three kinds of size particles are mixed by following percentage by weight: 50%, 40%, 10%, promptly obtain having different grain size proportioning feature, selenium content is the selenium-enriching cultivation feed additives of 50~100mg/kg.
(2) elegant precious mushroom is selenium nutrition-intensified:
In certain edible mushroom base, select additive for use as preparation as described in (1); The common cultivation material is formed: corncob 75%, cotton seed hulls 20%, quicklime 3%, land plaster 1%, composite fertilizer 1%.When the cultivate material spice, ratio in 5% and common cultivation material mixing, making final selenium content is 2.5~5mg/kg; Press material-water ratio 1: 1.3 and add running water, fully stir, mixing once more, heap fermentation, the steam sterilization sterilization treatment use in the pack back, makes final composts or fertilisers of cultivating inoculation, a bacterium; Cultivate according to precious mushroom cultivation of normal show and management method.
Implementation result:
Correct damp mushroom output is added up, and finds selenium nutrition-intensified group than common cultivation group output raising 20%.When each damp mushroom is ripe, pluck, carry out the mensuration of total selenium, organic selenium: total Se content is in 1.0~2.5mg/kg scope in the selenium enriched oyster massee fruiting bodies, and wherein the selenium that exists with the seleno-amino acids form accounts for about 70% of total selenium.The whole growth of fruit body is in the cycle, the relative standard deviation RSD of selenium content can be strict controlled in 20%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD is higher than 30%) elegant precious mushroom of the additive cultivation of different grain size proportioning feature; Selenium content is more stable in the same stage fruit body, and RSD all is lower than 8%.
Frying on an equal basis under the condition, allowing 5 personnel that the precious mushroom of common show and selenium enriched oyster mushroom mushroom meat mouthfeel, local flavor are assessed, the result shows: selenium enriched oyster mushroom quality is more tender and crisp, taste is more delicious.
Embodiment 4 elegant precious mushroom selenium-enriching cultivation embodiment
(1) preparation of selenium enriched oyster mushroom additive:
1. with a kind of crops leftovers, corncob by screening, dry, to be crushed to particle diameter be 0.005~0.1mm, the prescription of the required cultivate material additive of the precious mushroom of design cultivation of selenium-rich show.
This additive formulations is as follows:
100 parts of maize cob meals;
0.06~0.12 part of sodium selenate.
2. above-mentioned sodium selenate being ground to particle diameter is 0.005~0.1mm powder, with the abundant stirring and evenly mixing of maize cob meal, makes particle diameter below 0.1mm, and selenium content is elementary compound in 100~500mg/kg scope.
3. above-mentioned compound processing granulation, make it to become the different particle of granularity, its particle diameter is controlled at respectively in 0.005~0.1mm, 0.1~1.0mm, the 1.0~5.0mm scope.
4. above-mentioned three kinds of size particles are mixed by following percentage by weight: 50%, 30%, 20%, promptly obtain having different grain size proportioning feature, selenium content is the selenium-enriching cultivation feed additives of 100~500mg/kg.
(2) elegant precious mushroom is selenium nutrition-intensified:
In certain edible mushroom base, select additive for use as preparation as described in (1); The common cultivation material is formed: corncob 75%, cotton seed hulls 20%, quicklime 3%, land plaster 1%, composite fertilizer 1%.When the cultivate material spice, ratio in 10% and common cultivation material mixing, making final selenium content is that 10~50mg/kg adds running water by material-water ratio at 1: 1.3, fully stir mixing once more, heap fermentation, the steam sterilization sterilization treatment is used in the pack back, makes final composts or fertilisers of cultivating inoculation, sends out bacterium; Cultivate according to precious mushroom cultivation of normal show and management method.
Implementation result:
Correct damp mushroom output is added up, and finds selenium nutrition-intensified group than common cultivation group output raising 20%.When each damp mushroom is ripe, pluck, carry out the mensuration of total selenium, organic selenium: total Se content is in 4~10mg/kg scope in the selenium enriched oyster massee fruiting bodies, and wherein the selenium that exists with the seleno-amino acids form accounts for about 70% of total selenium.The whole growth of fruit body is in the cycle, the relative standard deviation RSD of selenium content can be strict controlled in 20%, and fluctuation is far below common cultivation (RSD is higher than 60%) and do not possess (RSD is higher than 30%) elegant precious mushroom of the additive cultivation of different grain size proportioning feature; Selenium content is more stable in the same stage fruit body, and RSD all is lower than 8%.
Frying on an equal basis under the condition, allowing 5 personnel that the precious mushroom of common show and selenium enriched oyster mushroom mushroom meat mouthfeel, local flavor are assessed, the result shows: selenium enriched oyster mushroom quality is more tender and crisp, taste is more delicious.
Above-mentioned example only is explanation technical conceive of the present invention and characteristics, and its purpose is to allow the people who is familiar with this technology can understand content of the present invention and enforcement according to this, can not limit protection scope of the present invention with this.All spirit essence is done according to the present invention equivalent transformation or modification all should be encompassed within protection scope of the present invention.

Claims (9)

1. method of utilizing sodium selenate to carry out the precious mushroom of even selenium-enriching cultivation show, it is characterized in that described method is included in cultivation and sneaks into 1~10% the selenium-enriching cultivation feed additives that accounts for cultivation siccative gross weight, the step of cultivating the elegant precious mushroom that obtains even rich selenium according to the common cultivation method in the siccative; Wherein to contain the selenium total amount be 5~500mg/kg to the selenium-enriching cultivation feed additives, and grain diameter is many particle size region and distributes in the selenium-enriching cultivation feed additives in 0.005~5.0mm scope, and particle diameter is that the particle of 1.0~5.0mm accounts for 10% of additive gross weight at least; Particle diameter is that the particle of 0.005~0.1mm accounts for 40% of additive gross weight at least; The component raw material that described selenium-enriching cultivation feed additives adopts comprises in parts by weight separately:
Maize cob meal 100 parts by weight;
Sodium selenate 0.0012~0.12 parts by weight.
2. method according to claim 1 is characterized in that grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005~5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10~20% of 1.0~5.0mm;
Particle diameter is the particle 40~70% of 0.005~0.1mm;
The particle 20~40% of other particle size range.
3. method according to claim 2 is characterized in that grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005~5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 10% of 1.0~5.0mm;
Particle diameter is the particle 50% of 0.005~0.1mm;
The particle 40% of other particle size range.
4. method according to claim 2 is characterized in that grain diameter is three particle size region distributions in the described selenium-enriching cultivation feed additives in 0.005~5.0mm scope, comprises with its weight percent meter in additive:
Particle diameter is the particle 20% of 1.0~5.0mm;
Particle diameter is the particle 50% of 0.005~0.1mm;
The particle 30% of other particle size range.
5. method according to claim 1 is characterized in that described cultivation siccative comprises with the weight percent meter of its component:
Corncob 75%;
Cotton seed hulls 20%;
Quicklime 3%;
Land plaster 1%;
Composite fertilizer 1%.
6. method according to claim 1, it is characterized in that described common cultivation method comprises that the cultivation siccative of will sneak into the selenium-enriching cultivation feed additives adds running water according to material-water ratio at 1: 1.3, abundant stirring and evenly mixing, heap fermentation, the steam sterilization sterilization treatment is used in the pack back, makes the even selenium enriched oyster mushroom of final composts or fertilisers of cultivating inoculated and cultured.
7. method according to claim 1 is characterized in that the maize cob meal that described selenium-enriching cultivation feed additives uses forms as the pulverizing of natural crops offcuts corncob.
8. method according to claim 1, the particle that it is characterized in that described selenium-enriching cultivation feed additives is by finishing granulation process after maize cob meal and the sodium selenate raw material pulverizing.
9. method according to claim 1, when it is characterized in that being used to keep healthy the cultivation of medicine food selenium enriched oyster mushroom, the Se content of the selenium-enriching cultivation feed additives of use is 200~500mg/kg; When being used for the cultivation of daily edible selenium enriched oyster mushroom, the Se content of the selenium-enriching cultivation feed additives of use is 5~100mg/kg.
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CN104350947A (en) * 2014-11-03 2015-02-18 苏州硒谷科技有限公司 Method using high-selenium edible fungus to extract selenium-rich edible fungus leaching liquid
CN107371786A (en) * 2017-06-15 2017-11-24 柳城新天地生态农业发展有限公司 The method for improving Se content in elegant precious mushroom

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Denomination of invention: Method for culturing pleurotus geesteranus uniformly rich in selenium by utilizing sodium selenate

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