CN103211088A - Preparation method of sea cucumber bait - Google Patents
Preparation method of sea cucumber bait Download PDFInfo
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- CN103211088A CN103211088A CN2013101675739A CN201310167573A CN103211088A CN 103211088 A CN103211088 A CN 103211088A CN 2013101675739 A CN2013101675739 A CN 2013101675739A CN 201310167573 A CN201310167573 A CN 201310167573A CN 103211088 A CN103211088 A CN 103211088A
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Abstract
The invention provides a preparation method of a sea cucumber bait. The preparation method solves the technical problems of instable quality, unreasonable nutritional composition, immature production process, less microbe content, not ideal effect, and the like of a raw material. The preparation method comprises the following steps of: inoculating lactic acid bacteria strains, microzyme strains, photosynthetic bacteria strains and bacillus stains to aquatic feed yeasts, float sludge and zostera marina powder in purified seawater, wherein the balance is the purified seawater, and the content is in terms of weight percent; carrying out liquid-state fermentation on a mixture at room temperature; and adding vitamins, immune polysaccharide, an enzyme preparation, amino acid peptides and radix astragali polysaccharide to a product to prepare the sea cucumber bait. The preparation method provided by the invention can be used for preparing the sea cucumber bait.
Description
Technical field
The present invention relates to a kind of preparation method of aquatic feed, particularly relate to a kind of preparation method of sea cucumber bait.
Background technology
In recent years, along with the continuous growth of successful, the raising of culturing level of sea cucumber artificial breeding technique and domestic market to the finished product sea cucumber consumer group, make holothruian cultures already develop rapidly, the sea cucumber bait industry also develops rapidly thereupon, though the sea cucumber bait demand is big.But lack of material, second-rate, market confusion make sea cucumber bait become sea cucumber one of the limitation factor with breeding production of growing seedlings.
At present, sea cucumber juvenile stage bait is mainly with unicellular alga with substitute bait.Unicellular alga has Chaetoceros muelleri, little Nitzschia closterium minutissima, salt algae and chrysophyceae etc.It is best that salt algae and chrysophyceae and Chaetoceros mix the cultivation effect of throwing something and feeding.Substituting bait has ocean rhodotorula, false rhodotorula, spheric yeast, photosynthetic bacteria and tangleweed to grind liquid etc.A large amount of result of the tests prove: period of metamorphosis algae and ocean rhodotorula, and the later stage grinds the bait of liquid as the young with ocean rhodotorula and tangleweed, and nursery effect is good
Young ginseng and young ginseng stage bait only grind liquid, Articial bait making etc. with benthic diatom, tangleweed.The kind that tangleweed grinds liquid has mouse tail algae, sargassum and the Zostera marina effect of throwing something and feeding best at present.With tangleweed grind liquid and Articial bait making by fermentation as young ginseng bait, all obtain good result.
Become the bait in ginseng stage, be applicable to holothurian cultivation pond, seawater cage culture, tidal lag band aquifer cultivation, as long as use Articial bait making, tangleweed class that mouse tail algae, sargassum, Zostera marina etc. are arranged.The Articial bait making main component has fermented bean dregs fish meal, seawood meal, wheat bran, rice bran, corn protein powder, extra large mud scum etc.
The subject matter that bait exists in the finished product holothruian cultures is at present: (1) gained raw material quality instability, and result of use is undesirable; (2) the nutrition formation is unreasonable in the prescription, some nutrient deficiency; (3) production technology is immature, and is lack of standardization; (4) ooze of Shi Yonging and extra large mud scum are of poor quality, and content of microorganisms is few, and the bait effect that makes is undesirable.
For this reason, strengthen sea cucumber bait research, the nutriment bait that the microbial fermentation technology that constantly release energy, good palatability, can impel sea cucumber to grow fast is converted into sea cucumber is very necessary, to this, the scientific research of sea cucumber fermentative microorganism bait technology more and more is subjected to the attention at vast aquaculture family.
Summary of the invention
The present invention be exactly for solve existing sea cucumber bait raw material quality instability, nutrition constitutes technical problems such as unreasonable, that production technology is immature, content of microorganisms is few, effect is undesirable, provides that a kind of production technology is simple, raw material sources is in extensive and cost is low relatively, with short production cycle, the tunning conversion product is many, constant product quality, transform the production method of the finished product microbial fermentation bait for sea cucumbers of nutrition easily.
The preparation method of sea cucumber bait provided by the present invention, it contains the following step: with aquatic feeds yeast 12%～18%, extra large mud scum 22%～28%, Zostera marina powder 12%～18%, inoculating lactic acid bacterial classification 2%～6%, barms 2%～6%, photosynthetic bacteria kind 2%～6%, bacillus kind 2%～6% in purifying sea water, surplus is a purifying sea water, and above content is hundred parts of ratios of weight; (2) with said mixture 10～15 degrees centigrade of liquid state fermentations of room temperature 12～24 hours; (3) with product 1000～1200 weight portions that obtain in the step (2), add vitamin 0.8～1.5 weight portion, immune polysaccharide 1.5～7 weight portions, enzyme preparation 1.5～5 weight portions, amino acid peptide class 4～17 weight portions, astragalus polyose 0.8～5 weight portion, make sea cucumber bait.
Preferably, the lactobacillus inoculation in the step (1) is 5%, barms is 5%, the photosynthetic bacteria kind is 5%, the bacillus kind is 5%, and above content is hundred parts of ratios of weight.
Preferably, add product 1200 weight portions that obtain in the step (2) in the step (3), add vitamin 1.2 weight portions, immune polysaccharide 2 weight portions, enzyme preparation 3 weight portions, amino acid peptide class 5 weight portions, astragalus polyose 5 weight portions.
Among the present invention, the amino acid peptide class is main active such as physical adsorbability active material, main component MG, TI, SI, B, MO, CA, K, CO, SE, can directly be ingested by sea cucumber
After the related raw material fermentation, in seawater, can produce transaminase isoreactivity material after the activation, and become the flow-like holothurian cultivation pond of can directly splashing after the activation, in breeding water body, change water quality, the beneficial microbe colony of preponderating cultivates the high-quality bait that a large amount of probios can directly become sea cucumber.
After the related raw material fermentation, in the breeding water body of directly splashing, not influenced by temperature, salinity, acid-base value, wide accommodation, safe in utilization, non-stimulated, meet the strict demand of green product.
After the related raw material fermentation, can effectively reduce the ammonia nitrogen in the excreta, content of harmful such as nitrite reduce the pollution to water body environment, and the beneficial bacterium of discharging with ight soil can continue to improve water quality, keeps the water body long-term stability.
Bait after the fermentation has the food calling appetizing, and is aid digestion, anti-enteritis, and long-term use can reduce sea cucumber and " drag just, shakes the head " phenomenon.
Fermentation back bait can promote the seaweeds breeding, stablizes the algae phase, and then is made full use of by algae and protozoan, improves the rich of sea cucumber bait.
Biological feed of the present invention contains a large amount of mycoproteins, amylase isoreactivity bioprotein, contains the fragrant and sweet flavor of distinctive leavening acid.The amino acid composition balance of protein can adapt to holothruian cultures environment and biological nature, and suspension is stable in seawater, can directly be filtered by sea cucumber and assimilate.
Utilize sea cucumber in the research of microbial fermentation bait, must consider ecological condition and objective condition requirements such as the nutritional need of sea cucumber self and the rule of searching for food naturally that sea cucumber perches.Show according to a large amount of documents and science data: exist a large amount of microorganism species in the internal organ of sea cucumber, mainly based on beneficial bacteriums such as bacillus, saccharomycete, lactic acid bacteria, photosynthetic bacterias.All research fermentation bait major functions are: promote the absorption of enteron aisle to nutriment, promote the raising of immunity function, reduce the incidence of disease, improve survival rate, improve output and sea cucumber quality.
At present in the pool holothurian culture process, because the finished product sea cucumber bait that the raiser has thrown something and fed a large amount of, the deterioration and the eutrophication of holothurian cultivation pond bottom have been caused, the remaining bait of a large amount of sea cucumber ight soil produces poisonous and harmful substance in the holothurian pond bottom, produces too much ammonia, nitrogen, nitrite, hydrogen sulfide, heavy metal ion etc.
How the solution of reply variety of problems degrades, and the ecological environment balance is the key factor of holothurian cultivation pond in the maintenance water body, at the breed situation of present holothurian cultivation pond, adopts microbial fermentation technology that several characteristics is arranged:
1. can directly be searched for food by sea cucumber, can also be converted to the required nutritional labeling of sea cucumber, as multiple necessary amino acid, unrighted acid, vitamin and minerals, multiple digestive ferment and growth stimulant etc. as the bait of sea cucumber;
2. growth that can be good in seawater, harmful components such as the organic matter in the seawater body of degrading rapidly, liquid nitrogen, hydrogen sulfide purify water, and improve dissolved oxygen in the water body, prevent that the end is smelly;
3. can in water body and sea cucumber stomach, form absolute profitable strain;
4. can effectively suppress other harmful microbe breedings;
5. reduce the generation of harmful toxins in the sea cucumber stomach, improve immunity and the anti-stress ability of sea cucumber.
Make holothurian cultivation pond produce a large amount of beneficial bacteriums according to this breed prescription, under the prolific situation of beneficial bacterium, can produce unit cell algae, the benthic diatom of a large amount of seawater body positive energies,, improve sea cucumber output, quality, local flavor for sea cucumber provides a large amount of living body baits! The breeding of simultaneously a large amount of beneficial bacteriums provides advantage for the bottom enhancement of environment of holothurian pond.A large amount of beneficial bacteriums decompose the ight soil of sea cucumbers, have eliminated the generation of the harmful substances such as ammonia, nitrogen, nitrite of cultivating pool bottom.
From the grow seedlings practical problem of pattern, breeding production pattern and existence of sea cucumber, specifically find the sea cucumber juvenile stage: with unicellular alga, ocean rhodotorula and the legumin powder little peptide bait that ferments; Young ginseng and young ginseng stage: grind liquid, ocean rhodotorula and legumin powder little peptide bait and the compound bacteria fermentation mixed bait that ferments with tangleweed.At this three phases, adopt microbial fermentation bait to feed the sea cucumber young, young ginseng, children's ginseng, obtained ideal effect.
According to the sea cucumber that this method is cultured, can improve the per unit area yield per mu yield and reach more than 500 kilograms.
Experiment 1:
The present invention becomes the bright 50 mu of sweeping experiments of mountain forest field holothurian cultivation pond regular meeting through Rongcheng City, Shandong Province.Press the inventive method biofermentation full pool spilling head through 2011～2012 annual experimentations, the required nutritional need of the sea cucumber of cultivating in the pond waters reaches perfect condition, the good harvest of sea cucumber per unit area yield per mu yield more than 500 jin, and improved the quality of sea cucumber.
Experiment 2:
The present invention, implements by method of the present invention through 2011～2012 annual experimentations through 100 mu of sweeping experiments of the Changxing Island Yang Lai of Daliang City, Liaoning Province Longhai City ginseng cultivating pool, and the per mu yield per unit area yield reaches has obtained desirable effect more than 500 jin.
The specific embodiment
In the following embodiments, bacterial classification is provided by Zhejiang Institute of Fresh Water Aquatic Products: barms model: Q/ZJAB20-2010; Lactobacillus species model: Q/ZJAB03-2008; Photosynthetic bacteria kind model: Q/ZJAB15-2005; Bacillus kind model: Q/ZJAB02-2002.
The source of other composition is as follows: vitamin (Q/JK5002-2004), it mainly contains vitamin A .B family, immune polysaccharide (Q/JK079-2012), enzyme preparation (Q/JK069-2012), amino acid peptide class (Q/JK089-2012), astragalus polyose (Q/JK086-2012).Above manufacturer is Qingdao Kingcon bio tech ltd, is product type in the bracket.Other raw material is commercially available mill run or known products.
Embodiment 1:
Component and weight percent content is as follows: aquatic feeds yeast 12%, sea mud scum 22%, Zostera marina powder 12%, inoculating lactic acid bacterial classification 2%, barms 2%, photosynthetic bacteria kind 2%, bacillus kind 2% mixed bacteria powder activate in purifying sea water, surplus is a seawater, 10 degrees centigrade of liquid state fermentations of normal room temperature 12 hours produce sour-sweet yeast food calling smell.With 1000 kilograms of above-mentioned tunnings, add 0.8 kilogram of vitamin again, 1.5 kilograms of immune polysaccharides, 1.5 kilograms of enzyme preparations, 4 kilograms of amino acid peptide classes, 0.8 kilogram of astragalus polyose makes sea cucumber bait.
Embodiment 2:
Component and weight percent content is as follows: aquatic feeds yeast 15%, sea mud scum 25%, Zostera marina powder 15%, inoculating lactic acid bacterial classification 4%, barms 4%, photosynthetic bacteria kind 4%, bacillus kind 4% mixed bacteria powder activate in purifying sea water, surplus is a seawater, 10 degrees centigrade of liquid state fermentations of normal room temperature 12 hours produce sour-sweet yeast food calling smell.With 1000 kilograms of above-mentioned tunnings, add 1 kilogram of vitamin again, 2 kilograms of immune polysaccharides, 2 kilograms of enzyme preparations, 5 kilograms of amino acid peptide classes, 1 kilogram of astragalus polyose makes sea cucumber bait.
Embodiment 3:
Component and weight percent content is as follows: aquatic feeds yeast 18%, sea mud scum 28%, Zostera marina powder 18%, inoculating lactic acid bacterial classification 6%, barms 6%, photosynthetic bacteria kind 6%, bacillus kind 6% mixed bacteria powder activate in purifying sea water, surplus is a seawater, 10 degrees centigrade of liquid state fermentations of normal room temperature 12 hours produce sour-sweet yeast food calling smell.With 1000 kilograms of above-mentioned tunnings, add 1.2 kilograms of vitamins again, 2.5 kilograms of immune polysaccharides, 2.5 kilograms of enzyme preparations, 6 kilograms of amino acid peptide classes, 1.2 kilograms of astragalus polyoses make sea cucumber bait.
Embodiment 4:
Component and weight percent content is as follows: aquatic feeds yeast 12%, sea mud scum 22%, Zostera marina powder 12%, inoculating lactic acid bacterial classification 2%, barms 2% in purifying sea water, bacillus kind 2%, photosynthetic bacteria kind 2%, the Mixed Microbes powder activates, surplus is a seawater, 10 degrees centigrade of normal room temperatures can produce sour-sweet food calling smell in 24 hours with 1100 kilograms of above-mentioned tunnings with top fermentation, then added 1 kilogram of vitamin, 3 kilograms of immune polysaccharides, 2 kilograms of active enzyme preparations, 12 kilograms of amino acid peptide classes, 1 kilogram of astragalus polyose makes sea cucumber bait.
Embodiment 5:
Component and weight percent content is as follows: aquatic feeds yeast 15%, sea mud scum 25%, Zostera marina powder 15%, inoculating lactic acid bacterial classification 4%, barms 4% in purifying sea water, bacillus kind 4%, photosynthetic bacteria kind 4%, the Mixed Microbes powder activates, surplus is a seawater, and 12 degrees centigrade of normal room temperatures can produce sour-sweet food calling smell in 24 hours with top fermentation, with 1100 kilograms of above-mentioned tunnings, then add 1.2 kilograms of vitamins, 5 kilograms of immune polysaccharides, 3 kilograms of active enzyme preparations, 15 kilograms of amino acid peptide classes, 1.2 kilograms of astragalus polyoses make sea cucumber bait.
Embodiment 6:
Component and weight percent content is as follows: aquatic feeds yeast 18%, sea mud scum 28%, Zostera marina powder 18%, inoculating lactic acid bacterial classification 6%, barms 6% in purifying sea water, bacillus kind 6%, photosynthetic bacteria kind 6%, the Mixed Microbes powder activates, surplus is a seawater, and 12 degrees centigrade of normal room temperatures can produce sour-sweet food calling smell in 24 hours with top fermentation, with 1100 kilograms of above-mentioned tunnings, then add 1.5 kilograms of vitamins, 7 kilograms of immune polysaccharides, 5 kilograms of active enzyme preparations, 17 kilograms of amino acid peptide classes, 1.5 kilograms of astragalus polyoses make sea cucumber bait.
Embodiment 7:
Component and weight percent content is as follows: aquatic feeds yeast 12%, extra large mud scum 25%, Zostera marina powder 12%, add purifying sea water inoculating lactic acid kind 2%, barms 2%, photosynthetic bacteria kind 2%, bacillus kind 2%, surplus are seawater, temperature conventional fermentation of liquid more than 18 hours more than 15 degrees centigrade, with 1200 kilograms of above-mentioned tunnings, then add 1 kilogram of vitamin, 2 kilograms of immune polysaccharides, 2 kilograms of active enzyme preparations, 1 kilogram of astragalus polyose, 3 kilograms of amino acid peptides make sea cucumber bait.
Embodiment 8:
Component and weight percent content is as follows: aquatic feeds yeast 15%, extra large mud scum 30%, Zostera marina powder 15%, add purifying sea water inoculating lactic acid kind 4%, barms 4%, photosynthetic bacteria kind 4%, bacillus kind 4%, surplus are seawater, temperature conventional fermentation of liquid more than 18 hours more than 15 degrees centigrade, with 1200 kilograms of above-mentioned tunnings, then add 1.2 kilograms of vitamins, 3 kilograms of immune polysaccharides, 3 kilograms of active enzyme preparations, 2 kilograms of astragalus polyoses, 5 kilograms of amino acid peptides make sea cucumber bait.
Embodiment 9:
Component and weight percent content is as follows: aquatic feeds yeast 18%, extra large mud scum 35%, Zostera marina powder 18%, add purifying sea water inoculating lactic acid kind 6%, barms 6%, photosynthetic bacteria kind 6%, bacillus kind 6%, surplus are seawater, temperature conventional fermentation of liquid more than 18 hours more than 15 degrees centigrade, with 1200 kilograms of above-mentioned tunnings, then add 1.5 kilograms of vitamins, 4 kilograms of immune polysaccharides, 4 kilograms of active enzyme preparations, 3 kilograms of astragalus polyoses, 7 kilograms of amino acid peptides make sea cucumber bait.
Prescription in the foregoing description is to be 100 mu of pond waters by gross mass, is 667 cubes of water bodys by every mu of one meter depth of water of pond waters, and it is that 100,000 cubes of water bodys calculate (by the feeding volume that ferments every day) that 100 mu of holothurian cultivation ponds calculate water bodys by 1.5 meters.
Claims (3)
1. the preparation method of a sea cucumber bait is characterized in that containing the following step:
(1) with aquatic feeds yeast 12%～18%, extra large mud scum 22%～28%, Zostera marina powder 12%～18%, inoculating lactic acid bacterial classification 2%～6%, barms 2%～6%, photosynthetic bacteria kind 2%～6%, bacillus kind 2%～6% in purifying sea water, surplus is a purifying sea water, and above content is hundred parts of ratios of weight;
(2) with said mixture 10～15 degrees centigrade of liquid state fermentations of room temperature 12～24 hours;
(3) with product 1000～1200 weight portions that obtain in the step (2), add vitamin 0.8～1.5 weight portion, immune polysaccharide 1.5～7 weight portions, enzyme preparation 1.5～5 weight portions, amino acid peptide class 4～17 weight portions, astragalus polyose 0.8～5 weight portion, make sea cucumber bait.
2. the preparation method of sea cucumber bait according to claim 1 is characterized in that lactobacillus inoculation in the described step (1) is 5%, barms is 5%, the photosynthetic bacteria kind is 5%, the bacillus kind is 5%, and above content is hundred parts of ratios of weight.
3. according to the preparation method of the sea cucumber bait described in claim 1 or 2, it is characterized in that adding product 1200 weight portions that obtain in the step (2) in the described step (3), add vitamin 1.2 weight portions, immune polysaccharide 2 weight portions, enzyme preparation 3 weight portions, amino acid peptide class 5 weight portions, astragalus polyose 5 weight portions.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103652497A (en) * | 2013-12-18 | 2014-03-26 | 青岛百瑞吉生物工程有限公司 | Feed additive for aquatic organisms and preparation method of feed additive |
| CN104666326A (en) * | 2013-12-03 | 2015-06-03 | 天津市中敖饲料有限公司 | Composition capable of improving immunity of fish body and preparation method of composition |
| CN106332814A (en) * | 2016-08-19 | 2017-01-18 | 青岛润物源生物科技有限公司 | Fully-ecological holothurian cultivation method |
| CN107319207A (en) * | 2017-08-07 | 2017-11-07 | 青岛科亮生物工程有限公司 | A kind of sea cucumber bait of purifying sea water breeding water body |
| CN112998159A (en) * | 2021-04-01 | 2021-06-22 | 大连鑫玉龙海洋生物种业科技股份有限公司 | Easily-absorbed fermented sea cucumber bait capable of improving biological activity and preparation method thereof |
| CN113080336A (en) * | 2020-01-08 | 2021-07-09 | 大连鑫玉龙海洋生物种业科技股份有限公司 | Sea cucumber feed prepared by fermenting Chinese herbal medicines |
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| CN101912051A (en) * | 2010-07-13 | 2010-12-15 | 大连海洋大学 | Fermentation process of sea cucumber compound feed |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104666326A (en) * | 2013-12-03 | 2015-06-03 | 天津市中敖饲料有限公司 | Composition capable of improving immunity of fish body and preparation method of composition |
| CN103652497A (en) * | 2013-12-18 | 2014-03-26 | 青岛百瑞吉生物工程有限公司 | Feed additive for aquatic organisms and preparation method of feed additive |
| CN103652497B (en) * | 2013-12-18 | 2015-08-05 | 青岛百瑞吉生物工程有限公司 | A kind of aquatic biological feed additive and preparation method thereof |
| CN106332814A (en) * | 2016-08-19 | 2017-01-18 | 青岛润物源生物科技有限公司 | Fully-ecological holothurian cultivation method |
| CN107319207A (en) * | 2017-08-07 | 2017-11-07 | 青岛科亮生物工程有限公司 | A kind of sea cucumber bait of purifying sea water breeding water body |
| CN113080336A (en) * | 2020-01-08 | 2021-07-09 | 大连鑫玉龙海洋生物种业科技股份有限公司 | Sea cucumber feed prepared by fermenting Chinese herbal medicines |
| CN112998159A (en) * | 2021-04-01 | 2021-06-22 | 大连鑫玉龙海洋生物种业科技股份有限公司 | Easily-absorbed fermented sea cucumber bait capable of improving biological activity and preparation method thereof |
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Application publication date: 20130724 |