CN103652497A - Feed additive for aquatic organisms and preparation method of feed additive - Google Patents
Feed additive for aquatic organisms and preparation method of feed additive Download PDFInfo
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- CN103652497A CN103652497A CN201310699413.9A CN201310699413A CN103652497A CN 103652497 A CN103652497 A CN 103652497A CN 201310699413 A CN201310699413 A CN 201310699413A CN 103652497 A CN103652497 A CN 103652497A
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Abstract
The invention provides a feed additive for aquatic organisms and a preparation method of the feed additive. The feed additive is mainly prepared by raw materials in parts by weight as follows: 50 parts of fish meal, 20-40 parts of kelp powder, 2-10 parts of an amino acid additive, 2-10 parts of astragalus polysaccharides and 1-5 parts of vitamins through fermentation by bifidobacteria and lactobacilli. Compared with common feed additives in the prior art, the prepared feed additive for the aquatic organisms has the advantages that the bifidobacteria and the lactobacilli are used for fermentation, the metabolism of the aquatic organisms can be promoted, fat, fat-soluble vitamins, cholesterol and the like in food can be fully digested, and breeding of harmful germs can be inhibited, so that the growth cycle can be shortened, and the death rate can be reduced; and meanwhile, chemical drugs are avoided, and water environments can be protected.
Description
Technical field
The present invention relates to a kind of biology feed additive, be specifically related to a kind of aquatic biological feed additive that ferments made by multiple bacterium, be applied to agriculture field.
Background technology
From 20 century 70 middle and later periods, the development experience of China's feed industry emerging, grow up, adjust 3 stages, tentatively built up complete feed Industry research, production, popularization, marketing system, and obtained the achievement attracting people's attention.The Feed Industry Development of China is very fast, and forage yield increases sharply, and has greatly enriched the market supply of feed.China has become second-biggest-in-the-world production of fodder state, is only second to the U.S..In recent ten years, China's feed product output stable development, quality steadily improves, and structure is more rational, and aggregate level continues to improve.But China's feed industry also exists that scale and benefit are low, technical merit and managerial skills are lower, the uncontested advantage of production cost and labor productivity aspect, the market competitiveness are weak, the feed industrial structure and layout is unreasonable and the problem such as feed safety.
Common aquatic biological feed comprises the farm manure of livestock and poultry urine excrement or the fertilizer of process decomposition reactor system, although these fertilizer can play certain rich water effect, nutriment cannot fully decompose absorption, simultaneously, can destroy water ecology balance to a certain extent, cause water environment pollution.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the invention provides a kind of aquatic biological feed additive.
Technical scheme: for achieving the above object, the invention provides a kind of aquatic biological feed additive, it is mainly made through Bifidobacterium and lactobacillus fermentation by the raw material of following weight ratio:
50 parts of fish meal, seawood meal 20-40 part, amino acid additive 2-10 part, astragalus polyose 2-10 part, vitamin 1-5 part.
Preferably, above-mentioned aquatic biological feed additive is mainly made through Bifidobacterium and lactobacillus fermentation by the raw material of following weight ratio:
50 parts of fish meal, seawood meal 25-35 part, amino acid additive 4-8 part, astragalus polyose 4-8 part, vitamin 2-4 part.
Preferably, described amino acid additive is one or more in lysine, threonine, methionine, tryptophan.
Preferably, described vitamin is one or more in VitAVitE and vitamin C.
The present invention also provides the preparation method of above-mentioned aquatic biological feed additive, and it is mainly made by following steps:
(1) preparation of Medium of Bifidobacterium: in weight portion, get 80 parts of spirulina powders, 40 parts of stachyoses, 30 parts of powdered beefs, 30 parts of dusty yeasts, 20 parts of tomato juices, 20 parts of glucose, join in 1000 parts of distilled water, heating for dissolving, regulating pH is 7.2, and after packing, 121 ℃ of autoclaving 20min, obtain;
(2) Bifidobacterium is inoculated in above-mentioned culture medium, inoculum concentration is 1 * 10
5-1 * 10
7cfu/ml, cultivates 40-50 hour for 30-40 ℃, obtains Bifidobacterium and cultivates bacterium liquid;
(3) preparation of Bacillus acidi lactici culture medium: in weight portion, get 10 parts of peptones, 5 parts of powdered beefs, 4 parts of dusty yeasts, 20 parts of glucose, 2 parts of dipotassium hydrogen phosphates, 20 parts of tomato juices, 5 parts of sodium acetates, 2 parts of Triammonium citrates, 15 parts of agar powders, join in 1000 parts of distilled water, heating for dissolving, regulating pH is 6.2, after packing, 121 ℃ of autoclaving 20min, obtain;
(4) Bacillus acidi lactici is inoculated in step (3) gained culture medium, inoculum concentration is 1 * 10
5-1 * 10
7cfu/ml, cultivates 5-10 hour for 20-30 ℃, obtains Bacillus acidi lactici and cultivates bacterium liquid;
(5) fish meal, seawood meal, amino acid additive are joined in fermentor together with vitamin, in the weight portion of fish meal, add 1000 parts of pure water, stir, must treat zymotic fluid;
(6) above-mentioned Bifidobacterium being cultivated to bacterium liquid and Bacillus acidi lactici cultivates bacterium liquid and is inoculated in step (3) gained and treats in zymotic fluid, cultivate bacterium liquid and treat that the volume ratio of zymotic fluid is 1:(80-120 for two kinds), 30-40 ℃ of fermentation 10-20 days, add astragalus polyose, stirring and dissolving, take out freeze drying, obtain.
Beneficial effect: the present invention is with respect to prior art, gained aquatic biological feed additive, compare with normal diet additive, utilize Bifidobacterium and lactobacillus fermentation, can promote aquatic animal metabolism, fully the fat in digest food, liposoluble vitamin, cholesterol etc., suppress growing of harmful germ, thereby shortening growth cycle, reduces the death rate; Meanwhile, without chemicals, can protect water body environment.
The specific embodiment
Below in conjunction with embodiment, the present invention is further described.
Embodiment 1:
Prescription: 50 parts of fish meal, 20 parts of seawood meals, 2 parts of lysines, 2 parts of astragalus polyoses, retinol1 part.
The preparation method of above-mentioned aquatic biological feed additive, it comprises the steps:
(1) preparation of Medium of Bifidobacterium: in weight portion, get 80 parts of spirulina powders, 40 parts of stachyoses, 30 parts of powdered beefs, 30 parts of dusty yeasts, 20 parts of tomato juices, 20 parts of glucose, join in 1000 parts of distilled water, heating for dissolving, regulating pH is 7.2, and after packing, 121 ℃ of autoclaving 20min, obtain;
(2) Bifidobacterium is inoculated in above-mentioned culture medium, inoculum concentration is 1 * 10
5cfu/ml, cultivates 40 hours for 30 ℃, obtains Bifidobacterium and cultivates bacterium liquid;
(3) preparation of Bacillus acidi lactici culture medium: in weight portion, get 10 parts of peptones, 5 parts of powdered beefs, 4 parts of dusty yeasts, 20 parts of glucose, 2 parts of dipotassium hydrogen phosphates, 20 parts of tomato juices, 5 parts of sodium acetates, 2 parts of Triammonium citrates, 15 parts of agar powders, join in 1000 parts of distilled water, heating for dissolving, regulating pH is 6.2, after packing, 121 ℃ of autoclaving 20min, obtain;
(4) Bacillus acidi lactici is inoculated in step (3) gained culture medium, inoculum concentration is 1 * 10
5cfu/ml, cultivates 5 hours for 20 ℃, obtains Bacillus acidi lactici and cultivates bacterium liquid;
(5) fish meal, seawood meal, lysine are joined in fermentor together with vitamin A, in the weight portion of fish meal, add 1000 parts of pure water, stir, must treat zymotic fluid;
(6) above-mentioned Bifidobacterium is cultivated to bacterium liquid and Bacillus acidi lactici and cultivated bacterium liquid and be inoculated in step (3) gained and treat in zymotic fluid, cultivate bacterium liquid and treat that the volume ratio of zymotic fluid is 1:80 for two kinds, 30 ℃ of fermentations 10 days, add astragalus polyose, stirring and dissolving, takes out freeze drying, obtains.
Embodiment 2:
Prescription: 3 parts of 50 parts of fish meal, 40 parts of seawood meals, 5 parts of threonines, 5 parts of methionine, 10 parts of astragalus polyoses, dehydroretinol part, vitamin C.
The preparation method of above-mentioned aquatic biological feed additive, it comprises the steps:
(1) preparation of Medium of Bifidobacterium: in weight portion, get 80 parts of spirulina powders, 40 parts of stachyoses, 30 parts of powdered beefs, 30 parts of dusty yeasts, 20 parts of tomato juices, 20 parts of glucose, join in 1000 parts of distilled water, heating for dissolving, regulating pH is 7.2, and after packing, 121 ℃ of autoclaving 20min, obtain;
(2) Bifidobacterium is inoculated in above-mentioned culture medium, inoculum concentration is 1 * 10
7cfu/ml, cultivates 50 hours for 40 ℃, obtains Bifidobacterium and cultivates bacterium liquid;
(3) preparation of Bacillus acidi lactici culture medium: in weight portion, get 10 parts of peptones, 5 parts of powdered beefs, 4 parts of dusty yeasts, 20 parts of glucose, 2 parts of dipotassium hydrogen phosphates, 20 parts of tomato juices, 5 parts of sodium acetates, 2 parts of Triammonium citrates, 15 parts of agar powders, join in 1000 parts of distilled water, heating for dissolving, regulating pH is 6.2, after packing, 121 ℃ of autoclaving 20min, obtain;
(4) Bacillus acidi lactici is inoculated in step (3) gained culture medium, inoculum concentration is 1 * 10
7cfu/ml, cultivates 10 hours for 30 ℃, obtains Bacillus acidi lactici and cultivates bacterium liquid;
(5) fish meal, seawood meal, threonine, methionine, vitamin A are joined in fermentor together with vitamin C, in the weight portion of fish meal, add 1000 parts of pure water, stir, must treat zymotic fluid;
(6) above-mentioned Bifidobacterium is cultivated to bacterium liquid and Bacillus acidi lactici and cultivated bacterium liquid and be inoculated in step (3) gained and treat in zymotic fluid, cultivate bacterium liquid and treat that the volume ratio of zymotic fluid is 1:120 for two kinds, 40 ℃ of fermentations 20 days, add astragalus polyose, stirring and dissolving, takes out freeze drying, obtains.
Embodiment 3:
Prescription: 1 part of 50 parts of fish meal, 30 parts of seawood meals, 6 parts of tryptophans, 6 parts of astragalus polyoses, retinol1 part, vitamin e1 part, vitamin C.
The preparation method of above-mentioned aquatic biological feed additive, it comprises the steps:
(1) preparation of Medium of Bifidobacterium: in weight portion, get 80 parts of spirulina powders, 40 parts of stachyoses, 30 parts of powdered beefs, 30 parts of dusty yeasts, 20 parts of tomato juices, 20 parts of glucose, join in 1000 parts of distilled water, heating for dissolving, regulating pH is 7.2, and after packing, 121 ℃ of autoclaving 20min, obtain;
(2) Bifidobacterium is inoculated in above-mentioned culture medium, inoculum concentration is 1 * 10
6cfu/ml, cultivates 45 hours for 35 ℃, obtains Bifidobacterium and cultivates bacterium liquid;
(3) preparation of Bacillus acidi lactici culture medium: in weight portion, get 10 parts of peptones, 5 parts of powdered beefs, 4 parts of dusty yeasts, 20 parts of glucose, 2 parts of dipotassium hydrogen phosphates, 20 parts of tomato juices, 5 parts of sodium acetates, 2 parts of Triammonium citrates, 15 parts of agar powders, join in 1000 parts of distilled water, heating for dissolving, regulating pH is 6.2, after packing, 121 ℃ of autoclaving 20min, obtain;
(4) Bacillus acidi lactici is inoculated in step (3) gained culture medium, inoculum concentration is 1 * 10
6cfu/ml, cultivates 8 hours for 25 ℃, obtains Bacillus acidi lactici and cultivates bacterium liquid;
(5) fish meal, seawood meal, tryptophan, vitamin A, vitamin C are joined in fermentor together with vitamin E, in the weight portion of fish meal, add 1000 parts of pure water, stir, must treat zymotic fluid;
(6) above-mentioned Bifidobacterium is cultivated to bacterium liquid and Bacillus acidi lactici and cultivated bacterium liquid and be inoculated in step (3) gained and treat in zymotic fluid, cultivate bacterium liquid and treat that the volume ratio of zymotic fluid is 1:100 for two kinds, 35 ℃ of fermentations 15 days, add astragalus polyose, stirring and dissolving, takes out freeze drying, obtains.
Embodiment 4:
Prescription: 50 parts of fish meal, 25 parts of seawood meals, 4 parts of threonines, 4 parts of astragalus polyoses, vitamin E2 part.
The preparation method of above-mentioned aquatic biological feed additive, it comprises the steps:
(1) preparation of Medium of Bifidobacterium: in weight portion, get 80 parts of spirulina powders, 40 parts of stachyoses, 30 parts of powdered beefs, 30 parts of dusty yeasts, 20 parts of tomato juices, 20 parts of glucose, join in 1000 parts of distilled water, heating for dissolving, regulating pH is 7.2, and after packing, 121 ℃ of autoclaving 20min, obtain;
(2) Bifidobacterium is inoculated in above-mentioned culture medium, inoculum concentration is 1 * 10
5cfu/ml, cultivates 42 hours for 32 ℃, obtains Bifidobacterium and cultivates bacterium liquid;
(3) preparation of Bacillus acidi lactici culture medium: in weight portion, get 10 parts of peptones, 5 parts of powdered beefs, 4 parts of dusty yeasts, 20 parts of glucose, 2 parts of dipotassium hydrogen phosphates, 20 parts of tomato juices, 5 parts of sodium acetates, 2 parts of Triammonium citrates, 15 parts of agar powders, join in 1000 parts of distilled water, heating for dissolving, regulating pH is 6.2, after packing, 121 ℃ of autoclaving 20min, obtain;
(4) Bacillus acidi lactici is inoculated in step (3) gained culture medium, inoculum concentration is 1 * 10
5cfu/ml, cultivates 6 hours for 22 ℃, obtains Bacillus acidi lactici and cultivates bacterium liquid;
(5) fish meal, seawood meal, threonine are joined in fermentor together with vitamin E, in the weight portion of fish meal, add 1000 parts of pure water, stir, must treat zymotic fluid;
(6) above-mentioned Bifidobacterium is cultivated to bacterium liquid and Bacillus acidi lactici and cultivated bacterium liquid and be inoculated in step (3) gained and treat in zymotic fluid, cultivate bacterium liquid and treat that the volume ratio of zymotic fluid is 1:90 for two kinds, 32 ℃ of fermentations 12 days, add astragalus polyose, stirring and dissolving, takes out freeze drying, obtains.
Embodiment 5:
Prescription: 50 parts of fish meal, 35 parts of seawood meals, 2 parts of lysines, 2 parts of methionine, 2 parts of threonines, 2 parts of tryptophans, 8 parts of astragalus polyoses, 4 parts of vitamin Es.
The preparation method of above-mentioned aquatic biological feed additive, it comprises the steps:
(1) preparation of Medium of Bifidobacterium: in weight portion, get 80 parts of spirulina powders, 40 parts of stachyoses, 30 parts of powdered beefs, 30 parts of dusty yeasts, 20 parts of tomato juices, 20 parts of glucose, join in 1000 parts of distilled water, heating for dissolving, regulating pH is 7.2, and after packing, 121 ℃ of autoclaving 20min, obtain;
(2) Bifidobacterium is inoculated in above-mentioned culture medium, inoculum concentration is 1 * 10
7cfu/ml, cultivates 48 hours for 37 ℃, obtains Bifidobacterium and cultivates bacterium liquid;
(3) preparation of Bacillus acidi lactici culture medium: in weight portion, get 10 parts of peptones, 5 parts of powdered beefs, 4 parts of dusty yeasts, 20 parts of glucose, 2 parts of dipotassium hydrogen phosphates, 20 parts of tomato juices, 5 parts of sodium acetates, 2 parts of Triammonium citrates, 15 parts of agar powders, join in 1000 parts of distilled water, heating for dissolving, regulating pH is 6.2, after packing, 121 ℃ of autoclaving 20min, obtain;
(4) Bacillus acidi lactici is inoculated in step (3) gained culture medium, inoculum concentration is 1 * 10
7cfu/ml, cultivates 9 hours for 28 ℃, obtains Bacillus acidi lactici and cultivates bacterium liquid;
(5) fish meal, seawood meal, lysine, methionine, threonine, tryptophan are joined in fermentor together with vitamin E, in the weight portion of fish meal, add 1000 parts of pure water, stir, must treat zymotic fluid;
(6) above-mentioned Bifidobacterium is cultivated to bacterium liquid and Bacillus acidi lactici and cultivated bacterium liquid and be inoculated in step (3) gained and treat in zymotic fluid, cultivate bacterium liquid and treat that the volume ratio of zymotic fluid is 1:110 for two kinds, 37 ℃ of fermentations 18 days, add astragalus polyose, stirring and dissolving, takes out freeze drying, obtains.
Experimental example gained aquatic biological feed of the present invention additive feeding effect is in Table 1
Choose 8 fry fields, every fry scene is amassed 1 mu, is divided at random 8 groups, is made as control group A, control group B control group C, 1 group of embodiment, 2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment, 5 groups of embodiment;
Control group A is used feed-interpolation bifidobacterium fermentation feed addictive, and described bifidobacterium fermentation feed addictive is in the present invention, to remove Bacillus acidi lactici and cultural method thereof, only uses bifidobacterium fermentation, the constant gained feed addictive of other compositions and method;
Control group B uses feed-interpolation lactobacillus fermentation feed addictive, and described lactobacillus fermentation feed addictive is in the present invention, to remove Bifidobacterium and cultural method thereof, only uses lactobacillus fermentation, the constant gained feed addictive of other compositions and method;
Control group C uses separately fermentation physics mixed fodder additive of feed-interpolation, and described independent fermentation physics mixed fodder additive refers to the simple physical mixture of feed addictive described in control group A and control group B;
Embodiment 1-5 group is used embodiment of the present invention 1-5 gained aquatic feeds.
Table 1 gained aquatic biological feed of the present invention additive is to fry growth experimental result
Note: with A control group, B control group and the comparison of C control group, * P<0.05
As seen from the above table, control group C compares with control group A, control group B, and growth cycle and survival rate all do not have marked change.And the aquatic biological feed additive of gained of the present invention is compared with control group A, B and C, growth cycle obviously shortens, and survival rate significantly improves.
Control group C compares with control group A, control group B, and in pH fluctuation situation and water, anoxic number of days does not all have marked change.And the aquatic biological feed additive of gained of the present invention is compared with control group A, B and C, in pH fluctuation and water, anoxic number of days all obviously reduces, and water quality situation is obviously stable.
Claims (5)
1. an aquatic biological feed additive, is characterized in that, it is mainly made through Bifidobacterium and lactobacillus fermentation by the raw material of following weight ratio:
50 parts of fish meal, seawood meal 20-40 part, amino acid additive 2-10 part, astragalus polyose 2-10 part, vitamin 1-5 part.
2. a kind of aquatic biological feed additive according to claim 1, is characterized in that, it is mainly made through Bifidobacterium and lactobacillus fermentation by the raw material of following weight ratio:
50 parts of fish meal, seawood meal 25-35 part, amino acid additive 4-8 part, astragalus polyose 4-8 part, vitamin 2-4 part.
3. a kind of aquatic biological feed additive according to claim 1, is characterized in that, described amino acid additive is one or more in lysine, threonine, methionine, tryptophan.
4. a kind of aquatic biological feed additive according to claim 1, is characterized in that, described vitamin is one or more in VitAVitE and vitamin C.
5. the preparation method of the aquatic biological feed additive described in claim 1-4 any one, is characterized in that, it is mainly made by following steps:
(1) preparation of Medium of Bifidobacterium: in weight portion, get 80 parts of spirulina powders, 40 parts of stachyoses, 30 parts of powdered beefs, 30 parts of dusty yeasts, 20 parts of tomato juices, 20 parts of glucose, join in 1000 parts of distilled water, heating for dissolving, regulating pH is 7.2, and after packing, 121 ℃ of autoclaving 20min, obtain;
(2) Bifidobacterium is inoculated in above-mentioned culture medium, inoculum concentration is 1 * 10
5-1 * 10
7cfu/ml, cultivates 40-50 hour for 30-40 ℃, obtains Bifidobacterium and cultivates bacterium liquid;
(3) preparation of Bacillus acidi lactici culture medium: in weight portion, get 10 parts of peptones, 5 parts of powdered beefs, 4 parts of dusty yeasts, 20 parts of glucose, 2 parts of dipotassium hydrogen phosphates, 20 parts of tomato juices, 5 parts of sodium acetates, 2 parts of Triammonium citrates, 15 parts of agar powders, join in 1000 parts of distilled water, heating for dissolving, regulating pH is 6.2, after packing, 121 ℃ of autoclaving 20min, obtain;
(4) Bacillus acidi lactici is inoculated in step (3) gained culture medium, inoculum concentration is 1 * 10
5-1 * 10
7cfu/ml, cultivates 5-10 hour for 20-30 ℃, obtains Bacillus acidi lactici and cultivates bacterium liquid;
(5) fish meal, seawood meal, amino acid additive are joined in fermentor together with vitamin, in the weight portion of fish meal, add 1000 parts of pure water, stir, must treat zymotic fluid;
(6) above-mentioned Bifidobacterium being cultivated to bacterium liquid and Bacillus acidi lactici cultivates bacterium liquid and is inoculated in step (3) gained and treats in zymotic fluid, cultivate bacterium liquid and treat that the volume ratio of zymotic fluid is 1:(80-120 for two kinds), 30-40 ℃ of fermentation 10-20 days, add astragalus polyose, stirring and dissolving, take out freeze drying, obtain.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101629150A (en) * | 2009-08-21 | 2010-01-20 | 中国科学院东北地理与农业生态研究所 | Compound bacteria additive for sugar beet pulp silages and preparation method and application thereof |
| CN102511847A (en) * | 2012-01-05 | 2012-06-27 | 中国人民解放军第三军医大学第一附属医院 | Preparation method of spirulina extract |
| CN102960570A (en) * | 2012-12-10 | 2013-03-13 | 北海市翰华生物技术有限公司 | Processing anti-corrosion method of aquatic feed |
| CN103211088A (en) * | 2013-05-08 | 2013-07-24 | 吕明涛 | Preparation method of sea cucumber bait |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101629150A (en) * | 2009-08-21 | 2010-01-20 | 中国科学院东北地理与农业生态研究所 | Compound bacteria additive for sugar beet pulp silages and preparation method and application thereof |
| CN102511847A (en) * | 2012-01-05 | 2012-06-27 | 中国人民解放军第三军医大学第一附属医院 | Preparation method of spirulina extract |
| CN102960570A (en) * | 2012-12-10 | 2013-03-13 | 北海市翰华生物技术有限公司 | Processing anti-corrosion method of aquatic feed |
| CN103211088A (en) * | 2013-05-08 | 2013-07-24 | 吕明涛 | Preparation method of sea cucumber bait |
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