CN103719597B - Aquatic organism feed and preparation method thereof - Google Patents
Aquatic organism feed and preparation method thereof Download PDFInfo
- Publication number
- CN103719597B CN103719597B CN201310697590.3A CN201310697590A CN103719597B CN 103719597 B CN103719597 B CN 103719597B CN 201310697590 A CN201310697590 A CN 201310697590A CN 103719597 B CN103719597 B CN 103719597B
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- Prior art keywords
- phytase
- bacillus subtilis
- parts
- feed
- lactobacillus acidophilus
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- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 29
- 108010011619 6-Phytase Proteins 0.000 claims abstract description 28
- 229940085127 phytase Drugs 0.000 claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 22
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 22
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 22
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 22
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 22
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 17
- 235000009566 rice Nutrition 0.000 claims abstract description 17
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 17
- 241000894006 Bacteria Species 0.000 claims description 27
- 238000000855 fermentation Methods 0.000 claims description 23
- 230000004151 fermentation Effects 0.000 claims description 23
- 241000209094 Oryza Species 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 14
- 239000007864 aqueous solution Substances 0.000 claims description 8
- 239000012467 final product Substances 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 7
- 230000009286 beneficial effect Effects 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 abstract description 6
- 102000004190 Enzymes Human genes 0.000 abstract description 6
- 229940088598 enzyme Drugs 0.000 abstract description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 4
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 4
- 239000011574 phosphorus Substances 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 abstract description 2
- 235000002949 phytic acid Nutrition 0.000 abstract description 2
- 241000233866 Fungi Species 0.000 abstract 1
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 244000052616 bacterial pathogen Species 0.000 abstract 1
- 238000009395 breeding Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 238000004904 shortening Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 235000021590 normal diet Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000006069 physical mixture Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Landscapes
- Fodder In General (AREA)
Abstract
The invention provides an aquatic organism feed and a preparation method thereof. The feed is prepared by the following components by weight: 0.5 part of bacillus subtilis, 0.1 to 0.7 part of candida utilis, 0.1 to 0.7 part of lactobacillus acidophilus, 30 to 60 parts of fungus chaff, 30 to 60 parts of green bran, 30 to 60 parts of wheat bran, 40 to 80 parts of rice bran, 10 to 30 parts of brown sugar, and 0.01 to 0.05 part of phytase. Compared with the ordinary feed in the prior art, the aquatic organism has the advantages that the bacillus subtilis, the candida utilis and the lactobacillus acidophilus, which are added to the aquatic organism feed, can produce various enzymes which can promote to absorb and digest aquatic animal food and restrain harmful germs from breeding, thereby shortening growth cycles and reducing death rate; at the same time, the phytase which is added can effectively decompose phytate phosphorus so as to use phosphorus sufficiently and protect water environment.
Description
Technical field
The present invention relates to a kind of biological feedstuff, be specifically related to a kind of primarily of the aquatic biological feed made by multiple bacterium and enzyme, be applied to agriculture field.
Background technology
From 20 century 70 middle and later periods, the development experience of China's feed industry is emerging, grow up, 3 stages of adjustment, tentatively built up complete feed industry scientific research, production, popularization, marketing system, and achieved the achievement attracted people's attention.The Feed Industry Development of China is very fast, and forage yield increases sharply, and has greatly enriched the market supply of feed.China has become second-biggest-in-the-world Feed Manufacturing state, is only second to the U.S..In recent ten years, China's feed product output stable development, quality steadily improves, and structure is more rational, and aggregate level continues to improve.But China's feed industry also also exists that scale and benefit are low, technical merit and managerial skills are lower, the uncontested advantage of production cost and labor productivity aspect, the market competitiveness are weak, feed industry structure and layout is unreasonable and the problem such as feed safety.
Common aquatic biological feed comprises the livestock and poultry urine farm manure of excrement or the fertilizer through decomposition reactor system, although these fertilizer can play certain rich water effect, nutriment fully cannot decompose absorption, simultaneously, water ecology balance can be destroyed to a certain extent, cause water environment pollution.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the invention provides a kind of aquatic biological feed.
Technical scheme: for achieving the above object, the invention provides a kind of aquatic biological feed, its primarily of following weight ratio component made by: bacillus subtilis 0.5 part, candida utili 0.1-07 part, lactobacillus acidophilus 0.1-0.7 part, bacterium chaff 30-60 part, blue or green chaff 30-60 part, wheat bran 30-60 part, rice bran 40-80 part, brown sugar 10-30 part, phytase 0.01-0.05 part.
Preferably, above-mentioned aquatic biological feed primarily of following weight ratio component made by: bacillus subtilis 0.5 part, candida utili 0.3-05 part, lactobacillus acidophilus 0.3-0.5 part, bacterium chaff 40-50 part, blue or green chaff 40-50 part, wheat bran 40-50 part, rice bran 50-70 part, brown sugar 15-25 part, phytase 0.02-0.04 part.
Preferably, described every gram of biological feedstuff contains beneficial microbe amount and is: bacillus subtilis is more than or equal to 7 hundred million, and candida utili is more than or equal to 7 hundred million, lactobacillus acidophilus is more than or equal to 5 hundred million.
Present invention also offers the preparation method of above-mentioned aquatic biological feed, it is made by following steps:
(1) bacterium chaff, blue or green chaff, wheat bran, rice bran, brown sugar add in fermentation tank, mix;
(2) bacillus subtilis mixes with the water of 500-700 times of volume, makes bacillus subtilis bacterium liquid, joins in above-mentioned fermentation tank, and fermentation 24-48 hour, obtains first time fermentate;
(3) candida utili and lactobacillus acidophilus mix with the water of 800-1200 times of volume, make mixed bacteria liquid, add in above-mentioned first time fermentate, continue fermentation 24-48 hour, obtain whole fermentate;
(4) phytase is mixed with the aqueous solution that concentration is 0.1-0.5%, described concentration is w/v;
(5) whole fermentate taking-up be dry, pulverize, spray into phytase solution, to obtain final product.
Beneficial effect: the present invention is relative to prior art, gained aquatic biological feed, compared with normal diet, the bacillus subtilis added, candida utili and lactobacillus acidophilus can produce multiple enzyme, for aquatic animal food absorption and digestion, there is facilitation, suppress growing of harmful levels of pathogens, thus shorten growth cycle, reduce the death rate; Meanwhile, add phytase, effectively can decompose phytate phosphorus, phosphorus is fully utilized, protection water body environment.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further described.
Embodiment 1:
Prescription: bacillus subtilis 0.5 part, candida utili 0.1 part, lactobacillus acidophilus 0.1 part, bacterium chaff 30 parts, blue or green chaff 30 parts, 30 parts, wheat bran, 40 parts, rice bran, 10 parts, brown sugar, phytase 0.01 part.
The preparation method of above-mentioned aquatic biological feed, it comprises the steps:
(1) bacterium chaff, blue or green chaff, wheat bran, rice bran, brown sugar add in fermentation tank, mix;
(2) bacillus subtilis mixes with the water of 500 times of volumes, makes bacillus subtilis bacterium liquid, joins in above-mentioned fermentation tank, ferments 24 hours, obtains first time fermentate;
(3) candida utili and lactobacillus acidophilus mix with the water of 800 times of volumes, make mixed bacteria liquid, add in above-mentioned first time fermentate, continue fermentation 24 hours, obtain whole fermentate;
(4) phytase is mixed with the aqueous solution that concentration is 0.1%, described concentration is w/v;
(5) whole fermentate taking-up be dry, pulverize, spray into phytase solution, to obtain final product.
Embodiment 2:
Prescription: bacillus subtilis 0.5 part, candida utili 07 part, lactobacillus acidophilus 0.7 part, bacterium chaff 60 parts, blue or green chaff 60 parts, 60 parts, wheat bran, 80 parts, rice bran, 30 parts, brown sugar, phytase 0.05 part.
The preparation method of above-mentioned aquatic biological feed, it comprises the steps:
(1) bacterium chaff, blue or green chaff, wheat bran, rice bran, brown sugar add in fermentation tank, mix;
(2) bacillus subtilis mixes with the water of 700 times of volumes, makes bacillus subtilis bacterium liquid, joins in above-mentioned fermentation tank, ferments 48 hours, obtains first time fermentate;
(3) candida utili and lactobacillus acidophilus mix with the water of 1200 times of volumes, make mixed bacteria liquid, add in above-mentioned first time fermentate, continue fermentation 48 hours, obtain whole fermentate;
(4) phytase is mixed with the aqueous solution that concentration is 0.5%, described concentration is w/v;
(5) whole fermentate taking-up be dry, pulverize, spray into phytase solution, to obtain final product.
Embodiment 3:
Prescription: bacillus subtilis 0.5 part, candida utili 0.4 part, lactobacillus acidophilus 0.4 part, bacterium chaff 45 parts, blue or green chaff 45 parts, 45 parts, wheat bran, 60 parts, rice bran, 20 parts, brown sugar, phytase 0.03 part.
The preparation method of above-mentioned aquatic biological feed, it comprises the steps:
(1) bacterium chaff, blue or green chaff, wheat bran, rice bran, brown sugar add in fermentation tank, mix;
(2) bacillus subtilis mixes with the water of 600 times of volumes, makes bacillus subtilis bacterium liquid, joins in above-mentioned fermentation tank, ferments 36 hours, obtains first time fermentate;
(3) candida utili and lactobacillus acidophilus mix with the water of 1000 times of volumes, make mixed bacteria liquid, add in above-mentioned first time fermentate, continue fermentation 36 hours, obtain whole fermentate;
(4) phytase is mixed with the aqueous solution that concentration is 0.3%, described concentration is w/v;
(5) whole fermentate taking-up be dry, pulverize, spray into phytase solution, to obtain final product.
Embodiment 4:
Prescription: bacillus subtilis 0.5 part, candida utili 0.3 part, lactobacillus acidophilus 0.3 part, bacterium chaff 40 parts, blue or green chaff 40 parts, 40 parts, wheat bran, 50 parts, rice bran, 15 parts, brown sugar, phytase 0.02 part.
The preparation method of above-mentioned aquatic biological feed, it comprises the steps:
(1) bacterium chaff, blue or green chaff, wheat bran, rice bran, brown sugar add in fermentation tank, mix;
(2) bacillus subtilis mixes with the water of 550 times of volumes, makes bacillus subtilis bacterium liquid, joins in above-mentioned fermentation tank, ferments 30 hours, obtains first time fermentate;
(3) candida utili and lactobacillus acidophilus mix with the water of 900 times of volumes, make mixed bacteria liquid, add in above-mentioned first time fermentate, continue fermentation 32 hours, obtain whole fermentate;
(4) phytase is mixed with the aqueous solution that concentration is 0.2%, described concentration is w/v;
(5) whole fermentate taking-up be dry, pulverize, spray into phytase solution, to obtain final product.
Embodiment 5:
Prescription: bacillus subtilis 0.5 part, candida utili 05 part, lactobacillus acidophilus 0.5 part, bacterium chaff 50 parts, blue or green chaff 50 parts, 50 parts, wheat bran, 70 parts, rice bran, 25 parts, brown sugar, phytase 0.04 part.
The preparation method of above-mentioned aquatic biological feed, it comprises the steps:
(1) bacterium chaff, blue or green chaff, wheat bran, rice bran, brown sugar add in fermentation tank, mix;
(2) bacillus subtilis mixes with the water of 650 times of volumes, makes bacillus subtilis bacterium liquid, joins in above-mentioned fermentation tank, ferments 40 hours, obtains first time fermentate;
(3) candida utili and lactobacillus acidophilus mix with the water of 1100 times of volumes, make mixed bacteria liquid, add in above-mentioned first time fermentate, continue fermentation 42 hours, obtain whole fermentate;
(4) phytase is mixed with the aqueous solution that concentration is 0.4%, described concentration is w/v;
(5) whole fermentate taking-up be dry, pulverize, spray into phytase solution, to obtain final product.
Experimental example gained aquatic biological feed of the present invention feeding effect is in table 1
Choose 8 fry fields, every fry scene amasss 1 mu, is divided into 8 groups at random, is set to control group A, control group B control group C, embodiment 1 group, embodiment 2 groups, embodiment 3 groups, embodiment 4 groups, embodiment 5 groups;
Control group A uses bacterium fermented feed, and described bacterium fermented feed is remove phytase in the present invention, other compositions and the constant gained feed of method;
Control group A uses enzyme preparation feed, and described enzyme preparation feed is remove bacillus subtilis, candida utili and lactobacillus acidophilus in the present invention, other compositions and the constant gained feed of method;
Control group C uses mixed fodder, and described mixed fodder is control group A bacteria used thereby fermented feed and control group B used enzyme preparation feed simple physical mixture;
Embodiment 1-5 group uses embodiment of the present invention 1-5 gained aquatic feeds.
Table 1 gained aquatic biological feed of the present invention is to fry growth experimental result
Note: compare with A control group, B control group and C control group, * P<0.05
As seen from the above table, control group C is compared with control group A, control group B, and growth cycle and survival rate all do not have marked change.And the aquatic biological feed of gained of the present invention, compare with control group A, B with C, growth cycle obviously shortens, and survival rate significantly improves.
Control group C is compared with control group A, control group B, and the pH anoxic number of days in situation and water that fluctuates all does not have marked change.And the aquatic biological feed of gained of the present invention, compare with control group A, B with C, pH fluctuates and in water, anoxic number of days all obviously reduces, and water quality situation is obviously stablized.
Claims (3)
1. improve water body pH to fluctuate and the aquatic biological feed of anaerobic condition, it is characterized in that: its primarily of following weight ratio component made by: bacillus subtilis 0.5 part, candida utili 0.1-07 part, lactobacillus acidophilus 0.1-0.7 part, bacterium chaff 30-60 part, blue or green chaff 30-60 part, wheat bran 30-60 part, rice bran 40-80 part, brown sugar 10-30 part, phytase 0.01-0.05 part;
Described every gram of biological feedstuff contains beneficial microbe amount: bacillus subtilis is more than or equal to 7 hundred million, and candida utili is more than or equal to 7 hundred million, lactobacillus acidophilus is more than or equal to 5 hundred million;
In described aquatic biological feed preparation process, phytase is mixed with the aqueous solution that concentration is 0.1-0.5%, described concentration is w/v.
2. a kind of aquatic biological feed according to claim 1, is characterized in that: its primarily of following weight ratio component made by: bacillus subtilis 0.5 part, candida utili 0.3-05 part, lactobacillus acidophilus 0.3-0.5 part, bacterium chaff 40-50 part, blue or green chaff 40-50 part, wheat bran 40-50 part, rice bran 50-70 part, brown sugar 15-25 part, phytase 0.02-0.04 part.
3. the preparation method of a kind of aquatic biological feed according to claim 1, is characterized in that: it is made by following steps:
(1) bacterium chaff, blue or green chaff, wheat bran, rice bran, brown sugar add in fermentation tank, mix;
(2) bacillus subtilis mixes with the water of 500-700 times of volume, makes bacillus subtilis bacterium liquid, joins in above-mentioned fermentation tank, and fermentation 24-48 hour, obtains first time fermentate;
(3) candida utili and lactobacillus acidophilus mix with the water of 800-1200 times of volume, make mixed bacteria liquid, add in above-mentioned first time fermentate, continue fermentation 24-48 hour, obtain whole fermentate;
(4) phytase is mixed with the aqueous solution that concentration is 0.1-0.5%, described concentration is w/v;
(5) whole fermentate taking-up be dry, pulverize, spray into phytase solution, to obtain final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310697590.3A CN103719597B (en) | 2013-12-18 | 2013-12-18 | Aquatic organism feed and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310697590.3A CN103719597B (en) | 2013-12-18 | 2013-12-18 | Aquatic organism feed and preparation method thereof |
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| Publication Number | Publication Date |
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| CN103719597A CN103719597A (en) | 2014-04-16 |
| CN103719597B true CN103719597B (en) | 2015-05-13 |
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| CN201310697590.3A Expired - Fee Related CN103719597B (en) | 2013-12-18 | 2013-12-18 | Aquatic organism feed and preparation method thereof |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689796A (en) * | 2015-11-13 | 2017-05-24 | 江苏银宝生物科技有限公司 | Black carp biological feed formula and processing method |
| CN107792929A (en) * | 2017-11-30 | 2018-03-13 | 宁夏润宝禾农业科技有限公司 | A kind of salt reduction alkali method |
| CN112167431A (en) * | 2020-09-29 | 2021-01-05 | 南京宝辉生物饲料有限公司 | Rotifer biological fermentation feed and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099543A (en) * | 2007-08-03 | 2008-01-09 | 吴奇能 | Aquatic biological feed and preparation method thereof |
| CN101253941A (en) * | 2008-04-07 | 2008-09-03 | 缪淑华 | Non-antibiotic microorganism fermentation aquatic product feed stuff |
| CN102429127A (en) * | 2011-11-30 | 2012-05-02 | 刘爱国 | Aquatic bio-feed |
-
2013
- 2013-12-18 CN CN201310697590.3A patent/CN103719597B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099543A (en) * | 2007-08-03 | 2008-01-09 | 吴奇能 | Aquatic biological feed and preparation method thereof |
| CN101253941A (en) * | 2008-04-07 | 2008-09-03 | 缪淑华 | Non-antibiotic microorganism fermentation aquatic product feed stuff |
| CN102429127A (en) * | 2011-11-30 | 2012-05-02 | 刘爱国 | Aquatic bio-feed |
Non-Patent Citations (1)
| Title |
|---|
| 微生物生物活性物质及其应用;王高学;《科学养鱼》;20060610(第06期);全文 * |
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Inventor after: Wang Xiaodong Inventor after: Chen Renxiao Inventor before: Bian Jialin |
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Effective date of registration: 20180105 Address after: 415137 Changde City, Hunan Province, West Dongting wish Fengzhen Ying Feng North Road East Side Industrial and commercial branch to the North 500 meters Patentee after: CHANGDE KEXIONG FODDER CO.,LTD. Address before: 266005 Shandong Province, Qingdao city Shenxian Road No. 39 room 219 Patentee before: QINGDAO BAIRUIJI BIOTECHNOLOGY Co.,Ltd. |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
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Granted publication date: 20150513 |