CN103319236B - Microorganism fish guano and preparation method thereof - Google Patents

Abstract

The invention discloses a microorganism fish guano and a preparation method thereof. The microorganism fish guano comprises the following components by weight part: 2-4 parts of bacillus subtilis powder, 1-4 parts of enzyme bacteria, 0.5-0.9 part of solid photosynthetic bacteria, 0.8-1.2 parts of bdellovibrio bacteriovorus, 0.2-0.6 part of a vitamin additive, 10-16 parts of a mineral substance additive, 0.2-0.4 part of a chelating agent, 60-80 parts of water hyacinth, 50-80 parts of edible fungi residue, 100-110 parts of vinasse, 40-60 parts of fishbone dust, 70-80 parts of rice bran and 10-16 parts of brown sugar. By adding the microorganism fish guano, rapid growth of fish is promoted, the quality of fish is improved, beneficial microorganisms in the water body are increased, and the occurrence of fish diseases can be effectively prevented and treated. The cultivation method is economical and effective.

Description

A kind of microbial fish guano and preparation method thereof

Technical field

The present invention relates to microbial fish guano technical field, be specifically related to a kind of microbial fish guano and preparation method thereof.

Background technology

Recent two decades comes, due to the development of intensive feeding cause, cause the sustainable growth of fish yield, wherein in cultivation, one of Main Yield-increasing measure of taking is (as the summer in suitable cultivation season, autumn) use urea to fishing pond at regular time and quantity, the nitrogen manure such as ammonium oxide and bicarbonate of ammonia, its effect is that fertilizer efficiency is high, can significantly increase filter-feeding freshwater fish, as silver carp, the planktonic growth that flathead is required, because pisciculture using chemical fertilizer has the effective feature such as pollution-free, particularly obtained universal and popularization rapidly in the freshwater aquiculture of China recent years at home and abroad.But long-term a large amount of chemical fertilizer that uses cultivates, and certainly will cause certain harm to the growth of fish, and the time has been grown meeting accumulation harmful element in fish body, thereby people are brought to certain harm.

At present a lot of plants adopt fertilizer, traditional fertilizer has its advantage, but shortcoming function singleness is longer by the process that plant plankton utilized, and in mineralisation process, consume a large amount of oxygen in water body, this in water to put product in a suitable place to breed as unfavorable in the growth such as fish; In addition, organic fish guano should be made base manure, and is mainly used in Winter-Spring water temperature lower season.And inorganic fertilizer function is comparatively single, although can make fish high yield, life-time service chemical fertilizer can bring many drawbacks, and contaminate environment is destroyed the eubiosis.

In fish farming process, in water body, can exist some to be unfavorable for the harmful bacteria of fish growth simultaneously, have a strong impact on the output of fish, thus in fish guano, add a certain amount of probiotics, effectively antagonism harmful bacteria, favourable to fish growth and breeding.Monistic compound fish guano can not be killed the harmful bacteria in fishpond at present, can not suppress the breeding growth of pathogenic bacteria in water body, therefore, lacks the effectively effect of control of fish disease.

Summary of the invention

The present invention is directed to the technical problem of current existence, a kind of microbial fish guano and preparation method thereof is provided, comprehensive nutrition is provided can on the one hand the growth of fish, can kill the harmful bacteria in fishpond on the other hand, suppress the breeding growth of pathogenic bacteria in water body, reduce the generation of disease, improve the yield and quality of fish.

For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:

A kind of microbial fish guano, comprises the composition of following weight ratio: bacillus subtilis bacteria powder 2-4 part, enzymatic microorganism 1-4 part, solid photosynthetic bacterium 0.5-0.9 part, bacteriophagic Bdellovibrio 0.8-1.2 part, VITAMIN addition material 0.2-0.6 part, mineral substance addition material 10-16 part, sequestrant 0.2-0.4 part, Herba Eichhorniae 60-80 part, edible fungi residue 50-80 part, vinasse 100-110 part, fishbone powder 40-60 part, rice bran 70-80 part, brown sugar 10-16 part.

Described VITAMIN addition material component recipe ratio is: vitamin A, C, E mix with the weight ratio of 2:3:5.

Described mineral substance addition material is potassium primary phosphate 20-25%, monocalcium phosphate 10-15%, ferrous sulfate 2-5%, zinc sulfate 1-2%, manganous sulfate 0.8-1%, borax 0.3-0.5%, rose vitriol 0.03-0.06%, rare earth 0.2-0.6%, and all the other add salt to 100%.

Described sequestrant is that sodium humate and EDTA mix with the weight ratio of 1:1-2.

The preparation method of mentioned microorganism fish guano, comprises the following steps:

(1) first Herba Eichhorniae, edible fungi residue, vinasse are dried in the sun to water content below 40%, then add fishbone powder, rice bran, brown sugar according to described ratio, fully stir, mix;

(2) in said mixture, add bacillus subtilis bacteria powder and the enzymatic microorganism of having dissolved according to described ratio, then above-mentioned substance is inserted and in pond, mix corruption and macerate, plastics film on upper cap, carries out fermenting in advance for 3-5 days; Then plastics film is thrown off, and stir, allow mixture contact oxygen, temperature gradually raises, from normal temperature to 45 DEG C, keep continuing turning after 2-3 days, be then raised to 60 DEG C from 45 DEG C, the hold-time is 3-5 days, then cool the temperature to normal temperature, keep 7-10 days, until slaking is completely fermented complete;

(3) solid photosynthetic bacterium, bacteriophagic Bdellovibrio are dissolved in water, join in said mixture, stir;

(4), by above-mentioned fermented product, air dry in the sun, makes moisture content of material be less than 25%, then pulverize, pulverize fineness for by without screen pulverizer, after pulverizing, in material, add mineral substance addition material, sequestrant and VITAMIN addition material, mix and obtain microbial fish guano.

Described bacillus subtilis bacteria powder, enzymatic microorganism, solid photosynthetic bacterium, bacteriophagic Bdellovibrio is all finished products of buying.

The invention has the beneficial effects as follows:

(1) added that fish produce needed organism and inorganic mineral, trace element can provide comprehensive nutrition to the growth of fish;

(2) add subtilis, enzymatic microorganism and bacteriophagic Bdellovibrio, can kill the harmful bacteria in fishpond, suppressed the breeding growth of pathogenic bacteria in water body, reduced the generation of disease, improved the yield and quality of fish.

(3) added solid photosynthetic bacterium, the toxic substance such as nitrite, sulfide that can degrade in water body, and can restrain oneself the organic waste water of high density, the poisonous substance such as phenol, cyanogen is had and necessarily stood and capacity of decomposition, there is stronger decomposition and inversion ability, reduce the accumulation of toxic substance in fish body.

(4) these mushrooms that add can continue breeding in water body, expand survival rate, the balance that maintains microorganisms in water is had a very important role, and can regulate and adjust microecological balance in fish body enteron aisle, can also decomposer in the Harmful Residue, strengthen fingerling disease resistance.

Embodiment

Below preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that, protection scope of the present invention is made to more explicit defining.

embodiment 1

A kind of microbial fish guano, comprises the composition of following weight ratio: 2 parts of bacillus subtilis bacteria powders, 1 part of enzymatic microorganism, 0.5 part of solid photosynthetic bacterium, 0.8 part of bacteriophagic Bdellovibrio, 0.2 part of VITAMIN addition material, 10 parts of mineral substance addition materials, 0.2 part of sequestrant, 60 parts of Herba Eichhorniaes, 50 parts of edible fungi residues, 100 parts, vinasse, 40 parts of fishbone powders, 70 parts, rice bran, 10 parts, brown sugar.

Described VITAMIN addition material component recipe ratio is: vitamin A, C, E mix with the weight ratio of 2:3:5.

Described mineral substance addition material is potassium primary phosphate 20%, monocalcium phosphate 10%, ferrous sulfate 2%, zinc sulfate 1%, manganous sulfate 0.8%, borax 0.3%, rose vitriol 0.03%, rare earth 0.2%, and all the other add salt to 100%.

Described sequestrant is that sodium humate and EDTA mix with the weight ratio of 1:1.

The preparation method of mentioned microorganism fish guano, comprises the following steps:

(1) first Herba Eichhorniae, edible fungi residue, vinasse are dried in the sun to water content below 40%, then add fishbone powder, rice bran, brown sugar according to described ratio, fully stir, mix;

(2) in said mixture, add bacillus subtilis bacteria powder and the enzymatic microorganism of having dissolved according to described ratio, then above-mentioned substance is inserted and in pond, mix corruption and macerate, plastics film on upper cap, carries out fermenting in advance for 3-5 days; Then plastics film is thrown off, and stir, allow mixture contact oxygen, temperature gradually raises, from normal temperature to 45 DEG C, keep continuing turning after 2-3 days, be then raised to 60 DEG C from 45 DEG C, the hold-time is 3-5 days, then cool the temperature to normal temperature, keep 7-10 days, until slaking is completely fermented complete;

(3) solid photosynthetic bacterium, bacteriophagic Bdellovibrio are dissolved in water, join in said mixture, stir;

(4), by above-mentioned fermented product, air dry in the sun, makes moisture content of material be less than 25%, then pulverize, pulverize fineness for by without screen pulverizer, after pulverizing, in material, add mineral substance addition material, sequestrant and VITAMIN addition material, mix and obtain microbial fish guano.

embodiment 2

A kind of microbial fish guano, comprises the composition of following weight ratio: bacillus subtilis bacteria powder 2-4 part, enzymatic microorganism 1-4 part, 0.7 part of solid photosynthetic bacterium, 1 part of bacteriophagic Bdellovibrio, 0.4 part of VITAMIN addition material, 12 parts of mineral substance addition materials, 0.3 part of sequestrant, 70 parts of Herba Eichhorniaes, 65 parts of edible fungi residues, 105 parts, vinasse, 50 parts of fishbone powders, 75 parts, rice bran, 12 parts, brown sugar.

Described VITAMIN addition material component recipe ratio is: vitamin A, C, E mix with the weight ratio of 2:3:5.

Described mineral substance addition material is potassium primary phosphate 22%, monocalcium phosphate 13%, ferrous sulfate 4%, zinc sulfate 2%, manganous sulfate 0.9%, borax 0.4%, rose vitriol 0.05%, rare earth 0.4%, and all the other add salt to 100%.

Described sequestrant is that sodium humate and EDTA mix with the weight ratio of 1:2.

Preparation method is with embodiment 1.

embodiment 3

A kind of microbial fish guano, comprises the composition of following weight ratio: 4 parts of bacillus subtilis bacteria powders, 4 parts of enzymatic microorganism, 0.9 part of solid photosynthetic bacterium, 1.2 parts of bacteriophagic Bdellovibrios, 0.6 part of VITAMIN addition material, 16 parts of mineral substance addition materials, 0.4 part of sequestrant, 80 parts of Herba Eichhorniaes, 80 parts of edible fungi residues, 110 parts, vinasse, 60 parts of fishbone powders, 80 parts, rice bran, 16 parts, brown sugar.

Described VITAMIN addition material component recipe ratio is: vitamin A, C, E mix with the weight ratio of 2:3:5.

Described mineral substance addition material is potassium primary phosphate 25%, monocalcium phosphate 15%; Ferrous sulfate 5%, zinc sulfate 2%, manganous sulfate 1%, borax 0.5%, rose vitriol 0.06%, rare earth 0.6%, all the other add salt to 100%.

Described sequestrant is that sodium humate and EDTA mix with the weight ratio of 1:2.

Preparation method is with embodiment 1.

The fish guano of preparation, concrete result of use is as follows:

Effect and the quality impact of the present invention on silver carp fingerling growth then

In the time that Reservoir Water Quality transparency is 50-80cm, the juvenile stage that sashimi (raw fish) is long, fish is pulled out, divide and support in the middle of five ponds, 200, every pond, in four ponds, throw in respectively microbial fish guano and the (contrast 1 of common biological fish manure of embodiment of the present invention 1-3 according to the injected volume of every mu of 200kg, originate from Beijing Century AMMS Biotechnology Co., Ltd.), another one pond is not thrown in fish guano (contrast 2) but is thrown in the common fish food of same amount, remaining feeding amount is identical, culture environment is identical, through the cultivation of 2-3 month, contrast the upgrowth situation of its output and fish, specifically as shown in Table 1 and Table 2:

Complex microorganism fish guano prepared by table 1 the present invention and contrast 1 are relatively

	Output increases (%)	Fish body increases (%)	EPA content increment (%)	Probiotics increment (%)
					Embodiment 1	14	13	21	25
Embodiment 2	18	14	30	35
					Embodiment 3	15	14	25	28

Microbial fish guano prepared by table 2 the present invention and contrast 2 are relatively

	Output increases (%)	Fish body increases (%)	EPA content increment (%)
				Embodiment 1	59	21	80
Embodiment 2	69	24	87
				Embodiment 3	64	22	84

Can find by above contrast, add the Fast Growth that microorganism fertilizer of the present invention not only can promote fish, can also improve quality and the quality of fish, but also the beneficial microorganism in water body is increased, the effectively generation of preventing and curing of fish disease is a kind of cost-effective cultural method.

The foregoing is only embodiments of the invention; not thereby limit the scope of the claims of the present invention; every equivalent structure or conversion of equivalent flow process that utilizes description of the present invention to do; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.

Claims (1)

1. a microbial fish guano, it is characterized in that, comprise the composition of following weight ratio: bacillus subtilis bacteria powder 2-4 part, enzymatic microorganism 1-4 part, solid photosynthetic bacterium 0.5-0.9 part, bacteriophagic Bdellovibrio 0.8-1.2 part, VITAMIN addition material 0.2-0.6 part, mineral substance addition material 10-16 part, sequestrant 0.2-0.4 part, Herba Eichhorniae 60-80 part, edible fungi residue 50-80 part, vinasse 100-110 part, fishbone powder 40-60 part, rice bran 70-80 part, brown sugar 10-16 part; Described VITAMIN addition material component recipe ratio is: vitamin A, C, E mix with the weight ratio of 2:3:5; Described mineral substance addition material is potassium primary phosphate 20-25%, monocalcium phosphate 10-15%, ferrous sulfate 2-5%, zinc sulfate 1-2%, manganous sulfate 0.8-1%, borax 0.3-0.5%, rose vitriol 0.03-0.06%, rare earth 0.2-0.6%, and all the other add salt to 100%; Described sequestrant is that sodium humate and EDTA mix with the weight ratio of 1:1-2; The preparation method of described a kind of microbial fish guano, is characterized in that, comprises the following steps:

(1) first Herba Eichhorniae, edible fungi residue, vinasse are dried in the sun to water content below 40%, then add fishbone powder, rice bran, brown sugar according to described ratio, fully stir, mix;

(2) in said mixture, add bacillus subtilis bacteria powder and the enzymatic microorganism of having dissolved according to described ratio, then above-mentioned substance is inserted and in pond, mix corruption and macerate, plastics film on upper cap, carries out fermenting in advance for 3-5 days; Then plastics film is thrown off, and stir, allow mixture contact oxygen, temperature gradually raises, from normal temperature to 45 DEG C, keep continuing turning after 2-3 days, be then raised to 60 DEG C from 45 DEG C, the hold-time is 3-5 days, then cool the temperature to normal temperature, keep 7-10 days, until slaking is completely fermented complete;

(3) solid photosynthetic bacterium, bacteriophagic Bdellovibrio are dissolved in water, join in said mixture, stir;

(4), by above-mentioned fermented product, air dry in the sun, makes moisture content of material be less than 25%, then pulverize, pulverize fineness for by without screen pulverizer, after pulverizing, in material, add mineral substance addition material, sequestrant and VITAMIN addition material, mix and obtain microbial fish guano.