CN103319236A - Microorganism fish guano and preparation method thereof - Google Patents

Abstract

The invention discloses a microorganism fish guano and a preparation method thereof. The microorganism fish guano comprises the following components by weight part: 2-4 parts of bacillus subtilis powder, 1-4 parts of enzyme bacteria, 0.5-0.9 part of solid photosynthetic bacteria, 0.8-1.2 parts of bdellovibrio bacteriovorus, 0.2-0.6 part of a vitamin additive, 10-16 parts of a mineral substance additive, 0.2-0.4 part of a chelating agent, 60-80 parts of water hyacinth, 50-80 parts of edible fungi residue, 100-110 parts of vinasse, 40-60 parts of fishbone dust, 70-80 parts of rice bran and 10-16 parts of brown sugar. By adding the microorganism fish guano, rapid growth of fish is promoted, the quality of fish is improved, beneficial microorganisms in the water body are increased, and the occurrence of fish diseases can be effectively prevented and treated. The cultivation method is economical and effective.

Description

A kind of microbial fish guano and preparation method thereof

Technical field

The present invention relates to the microbial fish guano technical field, be specifically related to a kind of microbial fish guano and preparation method thereof.

Background technology

Recent two decades comes, because the development of intensive feeding cause, caused the sustainable growth of fish yield, wherein in the cultivation one of Main Yield-increasing measure of taking be suitable cultivation season (such as the summer, autumn) uses urea to the fishing pond at regular time and quantity, the nitrogen manure such as ammonium oxide and bicarbonate of ammonia, its effect is that fertilizer efficiency is high, can significantly increase the filter-feeding freshwater fish, such as silver carp, the planktonic growth that flathead is required, because pisciculture using chemical fertilizer has the effective characteristics such as pollution-free, at home and abroad particularly obtained popularizing rapidly and promoting in the freshwater aquiculture of China recent years.But long-term a large amount of chemical fertilizer that uses cultivates, and certainly will cause the growth of fish certain harm, time to grow and can accumulate harmful element in the fish body, thereby people are brought certain harm.

At present a lot of plants adopt fertilizer, traditional fertilizer has its advantage, but the shortcoming function singleness is longer by the process that plant plankton utilized, and in mineralisation process, consume a large amount of oxygen in the water body, this in the water to put the growths such as product such as fish in a suitable place to breed unfavorable; In addition, organic fish guano should be made base manure, and is mainly used in Winter-Spring water temperature low season.And the inorganic fertilizer function is comparatively single, although can make the fish high yield, the life-time service chemical fertilizer can bring many drawbacks, and contaminate environment is destroyed the eubiosis.

In the fish farming process, can exist some to be unfavorable for the harmful bacteria of fish growth in the water body simultaneously, have a strong impact on the output of fish, so add a certain amount of probiotics in fish guano, effectively the antagonism harmful bacteria is favourable to fish growth and breeding.Monistic compound fish guano can not be killed the harmful bacteria in the fishpond at present, can not suppress the breeding growth of pathogenic bacteria in the water body, therefore, lacks the effectively effect of control of fish disease.

Summary of the invention

The present invention is directed to the technical problem of present existence, a kind of microbial fish guano and preparation method thereof is provided, comprehensive nutrition is provided on the one hand the growth of fish, can kill the harmful bacteria in the fishpond on the other hand, suppress the breeding growth of pathogenic bacteria in the water body, reduce the generation of disease, improve the yield and quality of fish.

For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:

A kind of microbial fish guano comprises the composition of following weight ratio: bacillus subtilis bacteria powder 2-4 part, enzymatic microorganism 1-4 part, solid photosynthetic bacterium 0.5-0.9 part, bacteriophagic Bdellovibrio 0.8-1.2 part, VITAMIN addition material 0.2-0.6 part, mineral substance addition material 10-16 part, sequestrant 0.2-0.4 part, Herba Eichhorniae 60-80 part, edible fungi residue 50-80 part, vinasse 100-110 part, fishbone powder 40-60 part, rice bran 70-80 part, brown sugar 10-16 part.

Described VITAMIN addition material component recipe ratio is: vitamin A, C, E mix with the weight ratio of 2:3:5.

Described mineral substance addition material is potassium primary phosphate 20-25%, monocalcium phosphate 10-15%, ferrous sulfate 2-5%, zinc sulfate 1-2%, manganous sulfate 0.8-1%, borax 0.3-0.5%, rose vitriol 0.03-0.06%, rare earth 0.2-0.6%, and all the other add salt to 100%.

To be sodium humate and EDTA mix with the weight ratio of 1:1-2 described sequestrant.

The preparation method of mentioned microorganism fish guano may further comprise the steps:

(1) at first Herba Eichhorniae, edible fungi residue, vinasse are dried in the sun to water content below 40%, then add fishbone powder, rice bran, brown sugar according to described ratio, fully stir, mix;

(2) add dissolving good bacillus subtilis bacteria powder and enzymatic microorganism according to described ratio in said mixture, then above-mentioned substance is inserted and mix corruption in the pond and macerate, plastics film on the upper cap carries out fermenting in advance in 3-5 days; Then plastics film is thrown off, and stir, allow mixture contact oxygen, temperature gradually raises, from normal temperature to 45 ℃, keep continuing turning after 2-3 days, then be raised to 60 ℃ from 45 ℃, the hold-time is 3-5 days, then cool the temperature to normal temperature, kept 7-10 days, until fully slaking is fermented complete;

(3) solid photosynthetic bacterium, bacteriophagic Bdellovibrio are dissolved in water, join in the said mixture, stir;

(4) with above-mentioned fermented product, air dry makes moisture content of material less than 25% in the sun, then pulverize, pulverize fineness for by without screen pulverizer, in material, add mineral substance addition material, sequestrant and VITAMIN addition material after pulverizing, mix and obtain microbial fish guano.

Described bacillus subtilis bacteria powder, enzymatic microorganism, solid photosynthetic bacterium, bacteriophagic Bdellovibrio all are finished products of buying.

The invention has the beneficial effects as follows:

(1) added that fish produce needed organism and inorganic mineral, trace element can provide comprehensive nutrition to the growth of fish;

(2) add subtilis, enzymatic microorganism and bacteriophagic Bdellovibrio, can kill the harmful bacteria in the fishpond, suppressed the breeding growth of pathogenic bacteria in the water body, reduced the generation of disease, improved the yield and quality of fish.

(3) added the solid photosynthetic bacterium, the toxic substances such as nitrite in the water body, sulfide of can degrading, and can restrain oneself the organic waste water of high density, the poisonous substances such as phenol, cyanogen are had necessarily stand and capacity of decomposition, have stronger decomposition and inversion ability, reduce the accumulation of toxic substance in the fish body.

(4) these mushrooms that add can continue breeding in water body, enlarge survival rate, the balance of keeping microorganisms in water is had a very important role, and can regulate and adjust microecological balance in the fish body enteron aisle, can also decomposer in the Harmful Residue, strengthen the fingerling disease resistance.

Embodiment

The below is described in detail preferred embodiment of the present invention, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that protection scope of the present invention is made more explicit defining.

Embodiment 1

A kind of microbial fish guano comprises the composition of following weight ratio: 2 parts of bacillus subtilis bacteria powders, 1 part of enzymatic microorganism, 0.5 part of solid photosynthetic bacterium, 0.8 part of bacteriophagic Bdellovibrio, 0.2 part of VITAMIN addition material, 10 parts of mineral substance addition materials, 0.2 part of sequestrant, 60 parts of Herba Eichhorniaes, 50 parts of edible fungi residues, 100 parts in vinasse, 40 parts of fishbone powders, 70 parts in rice bran, 10 parts in brown sugar.

Described VITAMIN addition material component recipe ratio is: vitamin A, C, E mix with the weight ratio of 2:3:5.

Described mineral substance addition material is potassium primary phosphate 20%, monocalcium phosphate 10%, ferrous sulfate 2%, zinc sulfate 1%, manganous sulfate 0.8%, borax 0.3%, rose vitriol 0.03%, rare earth 0.2%, and all the other add salt to 100%.

To be sodium humate and EDTA mix with the weight ratio of 1:1 described sequestrant.

The preparation method of mentioned microorganism fish guano may further comprise the steps:

(1) at first Herba Eichhorniae, edible fungi residue, vinasse are dried in the sun to water content below 40%, then add fishbone powder, rice bran, brown sugar according to described ratio, fully stir, mix;

(2) add dissolving good bacillus subtilis bacteria powder and enzymatic microorganism according to described ratio in said mixture, then above-mentioned substance is inserted and mix corruption in the pond and macerate, plastics film on the upper cap carries out fermenting in advance in 3-5 days; Then plastics film is thrown off, and stir, allow mixture contact oxygen, temperature gradually raises, from normal temperature to 45 ℃, keep continuing turning after 2-3 days, then be raised to 60 ℃ from 45 ℃, the hold-time is 3-5 days, then cool the temperature to normal temperature, kept 7-10 days, until fully slaking is fermented complete;

(3) solid photosynthetic bacterium, bacteriophagic Bdellovibrio are dissolved in water, join in the said mixture, stir;

(4) with above-mentioned fermented product, air dry makes moisture content of material less than 25% in the sun, then pulverize, pulverize fineness for by without screen pulverizer, in material, add mineral substance addition material, sequestrant and VITAMIN addition material after pulverizing, mix and obtain microbial fish guano.

Embodiment 2

A kind of microbial fish guano comprises the composition of following weight ratio: bacillus subtilis bacteria powder 2-4 part, enzymatic microorganism 1-4 part, 0.7 part of solid photosynthetic bacterium, 1 part of bacteriophagic Bdellovibrio, 0.4 part of VITAMIN addition material, 12 parts of mineral substance addition materials, 0.3 part of sequestrant, 70 parts of Herba Eichhorniaes, 65 parts of edible fungi residues, 105 parts in vinasse, 50 parts of fishbone powders, 75 parts in rice bran, 12 parts in brown sugar.

Described VITAMIN addition material component recipe ratio is: vitamin A, C, E mix with the weight ratio of 2:3:5.

Described mineral substance addition material is potassium primary phosphate 22%, monocalcium phosphate 13%, ferrous sulfate 4%, zinc sulfate 2%, manganous sulfate 0.9%, borax 0.4%, rose vitriol 0.05%, rare earth 0.4%, and all the other add salt to 100%.

To be sodium humate and EDTA mix with the weight ratio of 1:2 described sequestrant.

The preparation method is with embodiment 1.

Embodiment 3

A kind of microbial fish guano comprises the composition of following weight ratio: 4 parts of bacillus subtilis bacteria powders, 4 parts of enzymatic microorganism, 0.9 part of solid photosynthetic bacterium, 1.2 parts of bacteriophagic Bdellovibrios, 0.6 part of VITAMIN addition material, 16 parts of mineral substance addition materials, 0.4 part of sequestrant, 80 parts of Herba Eichhorniaes, 80 parts of edible fungi residues, 110 parts in vinasse, 60 parts of fishbone powders, 80 parts in rice bran, 16 parts in brown sugar.

Described VITAMIN addition material component recipe ratio is: vitamin A, C, E mix with the weight ratio of 2:3:5.

Described mineral substance addition material is potassium primary phosphate 25%, monocalcium phosphate 15%; Ferrous sulfate 5%, zinc sulfate 2%, manganous sulfate 1%, borax 0.5%, rose vitriol 0.06%, rare earth 0.6%, all the other add salt to 100%.

To be sodium humate and EDTA mix with the weight ratio of 1:2 described sequestrant.

The preparation method is with embodiment 1.

The fish guano of preparation, concrete result of use is as follows:

The present invention is on effect and the quality impact of then silver carp fingerling growth

When the Reservoir Water Quality transparency is 50-80cm, the juvenile stage that sashimi (raw fish) is long, fish is pulled out, divide and support in the middle of five ponds, 200 in every pond, in four ponds, throw in respectively microbial fish guano and the (contrast 1 of common biological fish manure of embodiment of the invention 1-3 according to the injected volume of every mu of 200kg, originate from Beijing Century AMMS Biotechnology Co., Ltd.), the another one pond is not thrown in fish guano (contrast 2) but is thrown in the common fish food of same amount, remaining feeding amount was identical, and culture environment is identical, through 2-3 month cultivation, contrast the upgrowth situation of its output and fish, specifically as shown in Table 1 and Table 2:

The complex microorganism fish guano of table 1 the present invention preparation and contrast 1 are relatively

	Output increases (%)	The fish body increases (%)	EPA content increment (%)	Probiotics increment (%)
					Embodiment 1	14	13	21	25
Embodiment 2	18	14	30	35
					Embodiment 3	15	14	25	28

The microbial fish guano of table 2 the present invention preparation and contrast 2 are relatively

	Output increases (%)	The fish body increases (%)	EPA content increment (%)
				Embodiment 1	59	21	80
Embodiment 2	69	24	87
				Embodiment 3	64	22	84

Can find by above contrast, add the Fast Growth that microorganism fertilizer of the present invention not only can promote fish, can also improve quality and the quality of fish, but also the beneficial microorganism in the water body is increased, the effectively generation of preventing and curing of fish disease is a kind of cost-effective cultural method.

The above only is embodiments of the invention; be not so limit claim of the present invention; every equivalent structure or equivalent flow process conversion that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the present invention.

Claims (5)

1. microbial fish guano, it is characterized in that, comprise the composition of following weight ratio: bacillus subtilis bacteria powder 2-4 part, enzymatic microorganism 1-4 part, solid photosynthetic bacterium 0.5-0.9 part, bacteriophagic Bdellovibrio 0.8-1.2 part, VITAMIN addition material 0.2-0.6 part, mineral substance addition material 10-16 part, sequestrant 0.2-0.4 part, Herba Eichhorniae 60-80 part, edible fungi residue 50-80 part, vinasse 100-110 part, fishbone powder 40-60 part, rice bran 70-80 part, brown sugar 10-16 part.

2. a kind of microbial fish guano according to claim 1 is characterized in that, described VITAMIN addition material component recipe ratio is: vitamin A, C, E mix with the weight ratio of 2:3:5.

3. a kind of microbial fish guano according to claim 1, it is characterized in that, described mineral substance addition material is potassium primary phosphate 20-25%, monocalcium phosphate 10-15%, ferrous sulfate 2-5%, zinc sulfate 1-2%, manganous sulfate 0.8-1%, borax 0.3-0.5%, rose vitriol 0.03-0.06%, rare earth 0.2-0.6%, and all the other add salt to 100%.

4. a kind of microbial fish guano according to claim 1 is characterized in that, to be sodium humate and EDTA mix with the weight ratio of 1:1-2 described sequestrant.

5. the preparation method of a kind of microbial fish guano according to claim 1 is characterized in that, may further comprise the steps:

(1) at first Herba Eichhorniae, edible fungi residue, vinasse are dried in the sun to water content below 40%, then add fishbone powder, rice bran, brown sugar according to described ratio, fully stir, mix;

(2) add dissolving good bacillus subtilis bacteria powder and enzymatic microorganism according to described ratio in said mixture, then above-mentioned substance is inserted and mix corruption in the pond and macerate, plastics film on the upper cap carries out fermenting in advance in 3-5 days; Then plastics film is thrown off, and stir, allow mixture contact oxygen, temperature gradually raises, from normal temperature to 45 ℃, keep continuing turning after 2-3 days, then be raised to 60 ℃ from 45 ℃, the hold-time is 3-5 days, then cool the temperature to normal temperature, kept 7-10 days, until fully slaking is fermented complete;

(3) solid photosynthetic bacterium, bacteriophagic Bdellovibrio are dissolved in water, join in the said mixture, stir;

(4) with above-mentioned fermented product, air dry makes moisture content of material less than 25% in the sun, then pulverize, pulverize fineness for by without screen pulverizer, in material, add mineral substance addition material, sequestrant and VITAMIN addition material after pulverizing, mix and obtain microbial fish guano.