CN103274831A - Method for preparing traditional Chinese medicine microbial fertilizer of spinach - Google Patents
Method for preparing traditional Chinese medicine microbial fertilizer of spinach Download PDFInfo
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- CN103274831A CN103274831A CN2013102289596A CN201310228959A CN103274831A CN 103274831 A CN103274831 A CN 103274831A CN 2013102289596 A CN2013102289596 A CN 2013102289596A CN 201310228959 A CN201310228959 A CN 201310228959A CN 103274831 A CN103274831 A CN 103274831A
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 29
- 235000009337 Spinacia oleracea Nutrition 0.000 title claims abstract description 26
- 230000000813 microbial effect Effects 0.000 title claims abstract description 16
- 239000003814 drug Substances 0.000 title claims abstract description 14
- 241000219315 Spinacia Species 0.000 title abstract description 25
- 238000000034 method Methods 0.000 title abstract description 6
- 244000005700 microbiome Species 0.000 claims abstract description 21
- 238000000855 fermentation Methods 0.000 claims abstract description 16
- 230000004151 fermentation Effects 0.000 claims abstract description 16
- 239000000654 additive Substances 0.000 claims abstract description 12
- 238000012545 processing Methods 0.000 claims abstract description 12
- 230000000996 additive effect Effects 0.000 claims abstract description 11
- 239000010902 straw Substances 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims abstract description 6
- 244000144977 poultry Species 0.000 claims abstract description 6
- 239000000463 material Substances 0.000 claims description 55
- 210000003608 fece Anatomy 0.000 claims description 26
- 239000010822 slaughterhouse waste Substances 0.000 claims description 25
- 239000010871 livestock manure Substances 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 21
- 241000287828 Gallus gallus Species 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 235000015097 nutrients Nutrition 0.000 claims description 13
- 238000002360 preparation method Methods 0.000 claims description 11
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 10
- 241000894006 Bacteria Species 0.000 claims description 10
- 229920000742 Cotton Polymers 0.000 claims description 10
- 244000068988 Glycine max Species 0.000 claims description 10
- 235000010469 Glycine max Nutrition 0.000 claims description 10
- 240000008042 Zea mays Species 0.000 claims description 10
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 10
- 238000005520 cutting process Methods 0.000 claims description 10
- 239000002054 inoculum Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- 235000009973 maize Nutrition 0.000 claims description 10
- 229910052760 oxygen Inorganic materials 0.000 claims description 10
- 239000001301 oxygen Substances 0.000 claims description 10
- 239000004033 plastic Substances 0.000 claims description 10
- 229920003023 plastic Polymers 0.000 claims description 10
- 235000015277 pork Nutrition 0.000 claims description 10
- 238000012216 screening Methods 0.000 claims description 10
- 239000000725 suspension Substances 0.000 claims description 10
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 239000011591 potassium Substances 0.000 claims description 7
- 229910052700 potassium Inorganic materials 0.000 claims description 7
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 6
- 229910052796 boron Inorganic materials 0.000 claims description 6
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 6
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 6
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 6
- 241000251468 Actinopterygii Species 0.000 claims description 5
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 5
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 5
- 241000187809 Frankia Species 0.000 claims description 5
- 241000187833 Geodermatophilus Species 0.000 claims description 5
- 235000008694 Humulus lupulus Nutrition 0.000 claims description 5
- ZSBXGIUJOOQZMP-UHFFFAOYSA-N Isomatrine Natural products C1CCC2CN3C(=O)CCCC3C3C2N1CCC3 ZSBXGIUJOOQZMP-UHFFFAOYSA-N 0.000 claims description 5
- ZSBXGIUJOOQZMP-JLNYLFASSA-N Matrine Chemical compound C1CC[C@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-JLNYLFASSA-N 0.000 claims description 5
- 241000218682 Pseudolarix amabilis Species 0.000 claims description 5
- 241001263604 Stellera chamaejasme Species 0.000 claims description 5
- 229910021529 ammonia Inorganic materials 0.000 claims description 5
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 5
- 230000009514 concussion Effects 0.000 claims description 5
- 239000000796 flavoring agent Substances 0.000 claims description 5
- 235000019634 flavors Nutrition 0.000 claims description 5
- 235000011194 food seasoning agent Nutrition 0.000 claims description 5
- 230000006872 improvement Effects 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 5
- 244000144972 livestock Species 0.000 claims description 5
- 229930014456 matrine Natural products 0.000 claims description 5
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 230000000630 rising effect Effects 0.000 claims description 5
- 210000000582 semen Anatomy 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 241000173529 Aconitum napellus Species 0.000 claims 1
- 244000300264 Spinacia oleracea Species 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 4
- 229910002651 NO3 Inorganic materials 0.000 abstract description 4
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 abstract description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 3
- 235000019154 vitamin C Nutrition 0.000 abstract description 3
- 239000011718 vitamin C Substances 0.000 abstract description 3
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 2
- 239000003899 bactericide agent Substances 0.000 abstract description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 abstract 2
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 abstract 1
- 229930003268 Vitamin C Natural products 0.000 abstract 1
- 238000009924 canning Methods 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 238000004090 dissolution Methods 0.000 abstract 1
- 239000002917 insecticide Substances 0.000 abstract 1
- 238000003307 slaughter Methods 0.000 abstract 1
- 239000002699 waste material Substances 0.000 abstract 1
- 239000002689 soil Substances 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 5
- 241000227129 Aconitum Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
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- 239000003895 organic fertilizer Substances 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
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- 238000005516 engineering process Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 230000007059 acute toxicity Effects 0.000 description 1
- 231100000403 acute toxicity Toxicity 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000000383 hazardous chemical Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 239000004021 humic acid Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
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- 230000008092 positive effect Effects 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Fertilizers (AREA)
Abstract
The invention discloses a method for preparing a traditional Chinese medicine microbial fertilizer of spinach. The method specifically comprises the steps as follows: fermented cultivation of microorganism, processing of crop straws, addition of poultry excrement and wastes of a slaughter house, fermentation treatment, dissolution of an additive, mixing, drying, smashing, canning for obtaining a finished product and the like. The microbial fertilizer prepared by the invention enables yield of spinach, content of vitamin C and iron element to be increased and enables content of nitrate to be decreased, so that the spinach quality is improved greatly; and the microbial fertilizer has functions of insecticide, bactericide and weed control, is pollution-free and no-residue and has better economic and social benefits.
Description
Technical field
The present invention relates to a kind of preparation method of microorganism fertilizer, particularly a kind of preparation method of spinach Chinese medicine microbial fertilizer belongs to fertilizer field.
Background technology
Spinach belongs to one of normal edible vegetables of people, has the iron of benefit effect, and is very popular.At present along with the raising of spinach cultivation technique and the increase of farmingization product consumption, the output of spinach also is greatly improved, but also produced secondary face effect simultaneously, as quality decline, taste variation, pollution aggravation etc., people are also more and more higher to the favor of organic spinach.But the output of organic vegetable is not high, does not satisfy people's demand.Its concrete reason one is fertilizer nutrient content instability, and the ratio of main nutrient elements is unreasonable, causes organic vegetable output to reduce significantly.The 2nd, some fertilizer contains objectionable impurities, such as, the fertilizer that with animal excrement is main material production contains the fodder additives residue, be that the fertilizer of main material production contains heavy metal etc. with sewage work's biochemical sludge, even if use these fertilizer spinach fully according to the operation of organic farm products production standard, its security of products also is difficult to guarantee.This just needs to change existing organic fertilizer production pattern, thereby makes the nutrient release in the fertilizer meet the requirement of spinach nutrition absorption, and is at present also rarely found to the research of spinach Chinese medicine microbial organic fertilizer.
Summary of the invention
The technical problem that the present invention mainly solves provides a kind of preparation method of spinach Chinese medicine microorganism fertilizer, can reduce the pollution of mineral manure, can also improve the yield and quality.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A kind of preparation method of spinach Chinese medicine microbial fertilizer may further comprise the steps:
(1) fermentation culture of microorganism:
1. bacillus amyloliquefaciens, bacillus thuringiensis, bacillusmusilaginosiengineering are inoculated on the inclined-plane of PDA substratum, the initial pH control of substratum is at 6-8; Culture temperature control is at 20-40 ℃; Cultivate and make bacteria suspension after 12-20 hour, more in proportion 2% inoculum size with the bacterial suspension inoculation for preparing in the PDA liquid nutrient medium, (the shaking table revolution is 160 rev/mins) cultivated in concussion, temperature is controlled at 25-30 ℃, cultivates 48-72 hour;
2. frankia, fish spore bacterium, Geodermatophilus are being improved on the Camp-BAP substratum, the initial pH control of substratum is about 7, temperature is 20-25 ℃, cultivated 12-16 days, the rising temperature continues to cultivate 10-14 days to 28-30 ℃, 1% inoculum size is inoculated in the improvement Camp-BAP liquid nutrient medium and ferments in proportion again, cultivates 72-96 hour under 25-30 ℃ of temperature;
(2) processing of crop material:
With maize straw, cotton stalk, soybean stalk spread out airing to moisture content below 10%; Then above-mentioned stalk is cut in cutting machine and smash, the length after the cutting is 1.5-2cm;
(3) add feces of livestock and poultry and slaughterhouse waste:
Adding weight ratio in the crop material after pulverizing is pig manure, chicken manure, pork slaughterhouse waste, the chicken slaughterhouse waste of 2:3:1:1; The weight ratio of adding above-mentioned substance and stalk is 50: 30-50 after the interpolation mixes both;
(4) fermentation: above-mentioned each material that will get ready is put into fermenter, evenly add cultured microorganism species simultaneously, and add brown sugar and water, mixture (maize straw, cotton stalk, soybean stalk, pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste): water: microorganism species: the weight ratio of brown sugar is 1000-1200:200-400:1:2; Throwing is turned in mechanize, with opening material, increase amount of oxygen, cultivate after 1-2 days, mixture is covered with plastics film, became thoroughly decomposed time 2-3 days, when finding that temperature surpasses 65 ℃, open film mixture carried out overturning, the timed interval be 1-2 days once, overturning 3-4 time, and then with the processing of plastics film jack, every square metre of slotting 10-20 1-2cm
2Sky, suitably strengthen amount of oxygen, temperature is controlled at 45-50 ℃, when finding that material is loose, does not have the original stink of material, and the ammonia flavor is arranged slightly, fermentation finishes when producing white hypha in the heap, obtains the fermented product that moisture content is 30-40%;
(5) dissolving of additive: be that the ratio of 2:1:3:4:3:4:4:2:1:3 is mixed with calcium superphosphate, boron mud, potassium fulvate, water, kongpo monkshood root, Semen Momordicae, Stellera chamaejasme L., hops, matrine, Golden Larch Bark according to weight ratio, stir;
(6) material in the step (4) is joined in the fermented product of step (5) generation, mix, the weight ratio of fermented product and additive is 80-100:1;
(7) oven dry, pulverize: above-mentioned material is dried through fluidized-bed, and temperature control is at 80 ℃ below the degree, and water content is controlled in the 10-15% scope, crushing screening, and fineness reaches 60 orders; Send into the cylinder tablets press after crushed with travelling belt then, uperize makes material be heated to 50-60 ℃ in cylinder, moves by not stall of cylinder and makes the material balling-up; Then drying roller is sent in balling-up, the input hot-air seasoning cools off, screening, canned one-tenth finished product again.
The invention has the beneficial effects as follows:
(1) raw material of Chinese medicine microorganism fertilizer of the present invention does not contain hazardous substance, and soil is not polluted, to product safety;
(2) bacterial classification that adopts of the present invention not only has effects such as fermented stalk and ight soil, and these microorganism species can continue the bacterial classification coexistence in breeding and the soil in being manured into soil, and has the protection plant, the effect of kill pests;
(3) utilize materials such as stalk that the microorganism species fermentative processing crosses and ight soil to pass through the stage of becoming thoroughly decomposed fully, have become thoroughly decomposed thoroughly, the sufficient characteristics of fermenting, more be conducive to the absorption of spinach;
(4) added materials such as calcium superphosphate, boron mud, potassium fulvate in microbial fertilizer, effectively solved spinach to the demand of phosphorus, calcium, potassium and other nutritive substance, made the Ye Gengda of spinach, output is higher.
(5) compared with prior art, it is good, environmentally safe that distinctive Chinese medicine formula synergy formulation of the present invention has a herbicidal effect, and weeds characteristics such as be difficult for developing immunity to drugs have a extensive future.Bactericidal and herbicidal agent of the present invention relatively safely to eyes, skin nonirritant, is low toxicity through bright its acute toxicity of day skin test checking to people and animals, easily decomposes in atmosphere and soil, and non-poisonous material is residual; The present invention is little to the influence of farmland natural enemy, helps to keep ecotope, and is more friendly to environment, satisfies the production requirement of crop.
Embodiment
Below preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that protection scope of the present invention is made more explicit defining.
Embodiment 1
A kind of preparation method of spinach Chinese medicine microbial fertilizer may further comprise the steps:
(1) fermentation culture of microorganism:
1. bacillus amyloliquefaciens, bacillus thuringiensis, bacillusmusilaginosiengineering are inoculated on the inclined-plane of PDA substratum, the initial pH control of substratum is 6; Culture temperature control is at 20 ℃; Cultivate and make bacteria suspension after 20 hours, more in proportion 2% inoculum size with the bacterial suspension inoculation for preparing in the PDA liquid nutrient medium, (the shaking table revolution is 160 rev/mins) cultivated in concussion, temperature is controlled at 30 ℃, cultivates 48 hours;
2. frankia, fish spore bacterium, Geodermatophilus are being improved on the Camp-BAP substratum, the initial pH control of substratum is about 7, temperature is 20 ℃, cultivated 16 days, ℃ continuation of rising temperature to 30 was cultivated 10 days, 1% inoculum size is inoculated in the improvement Camp-BAP liquid nutrient medium and ferments in proportion again, cultivates 72 hours under 25-30 ℃ of temperature;
(2) processing of crop material:
With maize straw, cotton stalk, soybean stalk spread out airing to moisture content below 10%; Then above-mentioned stalk is cut in cutting machine and smash, the length after the cutting is 1.5cm;
(3) add feces of livestock and poultry and slaughterhouse waste:
Adding weight ratio in the crop material after pulverizing is pig manure, chicken manure, pork slaughterhouse waste, the chicken slaughterhouse waste of 2:3:1:1; The weight ratio of adding above-mentioned substance and stalk is 50: 30, after the interpolation both is mixed;
(4) fermentation: above-mentioned each material that will get ready is put into fermenter, evenly add cultured microorganism species simultaneously, and add brown sugar and water, mixture (maize straw, cotton stalk, soybean stalk, pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste): water: microorganism species: the weight ratio of brown sugar is 1000:200:1:2; Throwing is turned in mechanize, with opening material, increase amount of oxygen, cultivate after 1 day, mixture is covered with plastics film, become thoroughly decomposed 3 days time, when finding that temperature surpasses 65 ℃, open film mixture carried out overturning, the timed interval be 1 day once, overturning 3-4 time, and then with the processing of plastics film jack, insert 10 2cm for every square metre
2Sky, suitably strengthen amount of oxygen, temperature is controlled at 45 ℃, when finding that material is loose, does not have the original stink of material, and the ammonia flavor is arranged slightly, fermentation finishes when producing white hypha in the heap, obtains moisture content and be 30% fermented product;
(5) dissolving of additive: be that the ratio of 2:1:3:4:3:4:4:2:1:3 is mixed with calcium superphosphate, boron mud, potassium fulvate, water, kongpo monkshood root, Semen Momordicae, Stellera chamaejasme L., hops, matrine, Golden Larch Bark according to weight ratio, stir;
(6) material in the step (4) is joined in the fermented product of step (5) generation, mix, the weight ratio of fermented product and additive is 80:1;
(7) oven dry, pulverize: above-mentioned material is dried through fluidized-bed, and temperature control is at 80 ℃ below the degree, and water content is controlled in the 10-15% scope, crushing screening, and fineness reaches 60 orders; Send into the cylinder tablets press after crushed with travelling belt then, uperize makes material be heated to 50 ℃ in cylinder, moves by not stall of cylinder and makes the material balling-up; Then drying roller is sent in balling-up, the input hot-air seasoning cools off, screening, canned one-tenth finished product again.
Embodiment 2
A kind of preparation method of spinach Chinese medicine microbial fertilizer may further comprise the steps:
(1) fermentation culture of microorganism:
1. bacillus amyloliquefaciens, bacillus thuringiensis, bacillusmusilaginosiengineering are inoculated on the inclined-plane of PDA substratum, the initial pH control of substratum is 7; Culture temperature control is at 30 ℃; Cultivate and make bacteria suspension after 16 hours, more in proportion 2% inoculum size with the bacterial suspension inoculation for preparing in the PDA liquid nutrient medium, (the shaking table revolution is 160 rev/mins) cultivated in concussion, temperature is controlled at 28 ℃, cultivates 56 hours;
2. frankia, fish spore bacterium, Geodermatophilus are being improved on the Camp-BAP substratum, the initial pH control of substratum is about 7, temperature is 22 ℃, cultivated 14 days, ℃ continuation of rising temperature to 29 was cultivated 12 days, 1% inoculum size is inoculated in the improvement Camp-BAP liquid nutrient medium and ferments in proportion again, cultivates 84 hours under 28 ℃ of temperature;
(2) processing of crop material:
With maize straw, cotton stalk, soybean stalk spread out airing to moisture content below 10%; Then above-mentioned stalk is cut in cutting machine and smash, the length after the cutting is 1.5cm;
(3) add feces of livestock and poultry and slaughterhouse waste:
Adding weight ratio in the crop material after pulverizing is pig manure, chicken manure, pork slaughterhouse waste, the chicken slaughterhouse waste of 2:3:1:1; The weight ratio of adding above-mentioned substance and stalk is 50: 40, after the interpolation both is mixed;
(4) fermentation: above-mentioned each material that will get ready is put into fermenter, evenly add cultured microorganism species simultaneously, and add brown sugar and water, mixture (maize straw, cotton stalk, soybean stalk, pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste): water: microorganism species: the weight ratio of brown sugar is 1100:300:1:2; Throwing is turned in mechanize, with opening material, increase amount of oxygen, cultivate after 2 days, mixture is covered with plastics film, become thoroughly decomposed 2 days time, when finding that temperature surpasses 65 ℃, open film mixture carried out overturning, the timed interval be 1 day once, overturning 4 times, and then with the processing of plastics film jack, insert 15 1.5cm for every square metre
2Sky, suitably strengthen amount of oxygen, temperature is controlled at 48 ℃, when finding that material is loose, does not have the original stink of material, and the ammonia flavor is arranged slightly, fermentation finishes when producing white hypha in the heap, obtains moisture content and be 35% fermented product;
(5) dissolving of additive: be that the ratio of 2:1:3:4:3:4:4:2:1:3 is mixed with calcium superphosphate, boron mud, potassium fulvate, water, kongpo monkshood root, Semen Momordicae, Stellera chamaejasme L., hops, matrine, Golden Larch Bark according to weight ratio, stir;
(6) material in the step (4) is joined in the fermented product of step (5) generation, mix, the weight ratio of fermented product and additive is 90:1;
(7) oven dry, pulverize: above-mentioned material is dried through fluidized-bed, and temperature control is at 80 ℃ below the degree, and water content is controlled in the 10-15% scope, crushing screening, and fineness reaches 60 orders; Send into the cylinder tablets press after crushed with travelling belt then, uperize makes material be heated to 55 ℃ in cylinder, moves by not stall of cylinder and makes the material balling-up; Then drying roller is sent in balling-up, the input hot-air seasoning cools off, screening, canned one-tenth finished product again.
Embodiment 3
1, a kind of preparation method of spinach Chinese medicine microbial fertilizer is characterized in that, may further comprise the steps:
(1) fermentation culture of microorganism:
1. bacillus amyloliquefaciens, bacillus thuringiensis, bacillusmusilaginosiengineering are inoculated on the inclined-plane of PDA substratum, the initial pH control of substratum is 8; Culture temperature control is at 40 ℃; Cultivate and make bacteria suspension after 12 hours, more in proportion 2% inoculum size with the bacterial suspension inoculation for preparing in the PDA liquid nutrient medium, (the shaking table revolution is 160 rev/mins) cultivated in concussion, temperature is controlled at 30 ℃, cultivates 48 hours;
2. frankia, fish spore bacterium, Geodermatophilus are being improved on the Camp-BAP substratum, the initial pH control of substratum is about 7, temperature is 25 ℃, cultivated 12 days, ℃ continuation of rising temperature to 28 was cultivated 14 days, 1% inoculum size is inoculated in the improvement Camp-BAP liquid nutrient medium and ferments in proportion again, cultivates 72 hours under 30 ℃ of temperature;
(2) processing of crop material:
With maize straw, cotton stalk, soybean stalk spread out airing to moisture content below 10%; Then above-mentioned stalk is cut in cutting machine and smash, the length after the cutting is 2cm;
(3) add feces of livestock and poultry and slaughterhouse waste:
Adding weight ratio in the crop material after pulverizing is pig manure, chicken manure, pork slaughterhouse waste, the chicken slaughterhouse waste of 2:3:1:1; The weight ratio of adding above-mentioned substance and stalk is 50: 50, after the interpolation both is mixed;
(4) fermentation: above-mentioned each material that will get ready is put into fermenter, evenly add cultured microorganism species simultaneously, and add brown sugar and water, mixture (maize straw, cotton stalk, soybean stalk, pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste): water: microorganism species: the weight ratio of brown sugar is 1200:400:1:2; Throwing is turned in mechanize, with opening material, increase amount of oxygen, cultivate after 2 days, mixture is covered with plastics film, become thoroughly decomposed 3 days time, when finding that temperature surpasses 65 ℃, open film mixture carried out overturning, the timed interval be 2 days once, overturning 3 times, and then with the processing of plastics film jack, insert 20 1cm for every square metre
2Sky, suitably strengthen amount of oxygen, temperature is controlled at 50 ℃, when finding that material is loose, does not have the original stink of material, and the ammonia flavor is arranged slightly, fermentation finishes when producing white hypha in the heap, obtains moisture content and be 40% fermented product;
(5) dissolving of additive: be that the ratio of 2:1:3:4:3:4:4:2:1:3 is mixed with calcium superphosphate, boron mud, potassium fulvate, water, kongpo monkshood root, Semen Momordicae, Stellera chamaejasme L., hops, matrine, Golden Larch Bark according to weight ratio, stir;
(6) material in the step (4) is joined in the fermented product of step (5) generation, mix, the weight ratio of fermented product and additive is 100:1;
(7) oven dry, pulverize: above-mentioned material is dried through fluidized-bed, and temperature control is at 80 ℃ below the degree, and water content is controlled in the 10-15% scope, crushing screening, and fineness reaches 60 orders; Send into the cylinder tablets press after crushed with travelling belt then, uperize makes material be heated to 60 ℃ in cylinder, moves by not stall of cylinder and makes the material balling-up; Then drying roller is sent in balling-up, the input hot-air seasoning cools off, screening, canned one-tenth finished product again.
The bio-organic fertilizer (contrast) that the microbial fertilizer that embodiment 1, embodiment 2, embodiment 3 are obtained and Guangzhou Nongguan Biotech Co., Ltd. produce is applied to spinach respectively, and spinach is unifiedly to plant outdoor cropping, unified management; The fertilizer amount of embodiment and contrast is identical.Respectively its output, vitamins C, ferro element, nitrate content and contrast are compared, obtain its comparing result.Specifically as table 1:
The microbial fertilizer of table 1 the present invention preparation and contrast fertilizer are relatively
 |
Output increment (%) | Vitamins C increment (%) | Ferro element increment (%) | Nitrate reduction (%) |
| Embodiment 1 | 12 | 10 | 15 | 30 |
| Embodiment 2 | 15 | 12 | 18 | 35 |
| Embodiment 3 | 13 | 9 | 13 | 26 |
The process contrast is found, the microbial fertilizer of the present invention's preparation can make the output of spinach, ascorbic content and ferro element all obtain increasing, and the reduction of the content of nitrate, and the quality of spinach is greatly enhanced.Agricultural chemicals seed dressing back seedling rate on average reaches 85.3%, and contrast treatment reaches conspicuous level, is 77.5% to the inhibiting rate of common disease; Of the present invention through the field simultaneous test, volume increase 20% all obtains positive effect, mainly shows as follows: contain and enrich organic matter and humic acid, can improve Soil structure, vegetable soil promotes soil to build up fertility.Reduce or the reduction crop pest with waiting other fertilizer ratio of nutrient, influence crop quality.Need not or reduce fertilizer amount, be beneficial to the protection environment.
The above only is embodiments of the invention; be not so limit claim of the present invention; every equivalent structure or equivalent flow process conversion that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the present invention.
Claims (1)
1. the preparation method of a spinach Chinese medicine microbial fertilizer is characterized in that, may further comprise the steps:
(1) fermentation culture of microorganism:
1. bacillus amyloliquefaciens, bacillus thuringiensis, bacillusmusilaginosiengineering are inoculated on the inclined-plane of PDA substratum, the initial pH control of substratum is at 6-8; Culture temperature control is at 20-40 ℃; Cultivate and make bacteria suspension after 12-20 hour, more in proportion 2% inoculum size with the bacterial suspension inoculation for preparing in the PDA liquid nutrient medium, concussion is cultivated, temperature is controlled at 25-30 ℃, cultivates 48-72 hour;
2. frankia, fish spore bacterium, Geodermatophilus are being improved on the Camp-BAP substratum, the initial pH control of substratum is about 7, temperature is 20-25 ℃, cultivated 12-16 days, the rising temperature continues to cultivate 10-14 days to 28-30 ℃, 1% inoculum size is inoculated in the improvement Camp-BAP liquid nutrient medium and ferments in proportion again, cultivates 72-96 hour under 25-30 ℃ of temperature;
(2) processing of crop material:
With maize straw, cotton stalk, soybean stalk spread out airing to moisture content below 10%; Then above-mentioned stalk is cut in cutting machine and smash, the length after the cutting is 1.5-2cm;
(3) add feces of livestock and poultry and slaughterhouse waste:
Adding weight ratio in the crop material after pulverizing is pig manure, chicken manure, pork slaughterhouse waste, the chicken slaughterhouse waste of 2:3:1:1; The weight ratio of adding above-mentioned substance and stalk is 50: 30-50 after the interpolation mixes both;
(4) fermentation: above-mentioned each material that will get ready is put into fermenter, evenly add cultured microorganism species simultaneously, and add brown sugar and water, mixture (maize straw, cotton stalk, soybean stalk, pig manure, chicken manure, pork slaughterhouse waste, chicken slaughterhouse waste): water: microorganism species: the weight ratio of brown sugar is 1000-1200:200-400:1:2; Throwing is turned in mechanize, with opening material, increase amount of oxygen, cultivate after 1-2 days, mixture is covered with plastics film, became thoroughly decomposed time 2-3 days, when finding that temperature surpasses 65 ℃, open film mixture carried out overturning, the timed interval be 1-2 days once, overturning 3-4 time, and then with the processing of plastics film jack, every square metre of slotting 10-20 1-2cm
2Sky, suitably strengthen amount of oxygen, temperature is controlled at 45-50 ℃, when finding that material is loose, does not have the original stink of material, and the ammonia flavor is arranged slightly, fermentation finishes when producing white hypha in the heap, obtains the fermented product that moisture content is 30-40%;
(5) dissolving of additive: be that the ratio of 2:1:3:4:3:4:4:2:1:3 is mixed with calcium superphosphate, boron mud, potassium fulvate, water, kongpo monkshood root, Semen Momordicae, Stellera chamaejasme L., hops, matrine, Golden Larch Bark according to weight ratio, stir;
(6) material in the step (4) is joined in the fermented product of step (5) generation, mix, the weight ratio of fermented product and additive is 80-100:1;
(7) oven dry, pulverize: above-mentioned material is dried through fluidized-bed, and temperature control is at 80 ℃ below the degree, and water content is controlled in the 10-15% scope, crushing screening, and fineness reaches 60 orders; Send into the cylinder tablets press after crushed with travelling belt then, uperize makes material be heated to 50-60 ℃ in cylinder, moves by not stall of cylinder and makes the material balling-up; Then drying roller is sent in balling-up, the input hot-air seasoning cools off, screening, canned one-tenth finished product again.
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| CN105439734A (en) * | 2015-12-23 | 2016-03-30 | 李春燕 | Fertilizer special for spinach and preparation method thereof |
| CN105646094A (en) * | 2016-03-08 | 2016-06-08 | 来安县亚海生态农业有限公司 | Soaking liquid for spinach planting |
| CN106495902A (en) * | 2016-10-25 | 2017-03-15 | 天津农学院 | A kind of bio-feritlizer of the discarded compost of utilization edible fungi and its preparation |
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| CN104909846A (en) * | 2015-05-18 | 2015-09-16 | 青岛根源生物技术集团有限公司 | Bacillus amyloliquefaciens HFJ-7-containing composite chicken manure decomposed starter and application thereof |
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| CN105439734A (en) * | 2015-12-23 | 2016-03-30 | 李春燕 | Fertilizer special for spinach and preparation method thereof |
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| CN109287977A (en) * | 2018-09-13 | 2019-02-01 | 西华大学 | A kind of process equipment of chicken snack food |
| CN111205144A (en) * | 2020-03-25 | 2020-05-29 | 杭州迹童精密机械有限公司 | Microbial fertilizer and preparation method thereof |
| CN114956893A (en) * | 2022-05-27 | 2022-08-30 | 四川帆田欣生物科技有限公司 | Preparation method of special endophytic mycorrhiza microbial fertilizer |
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