CN101928186A - Composite microbial fertilizer for controlling crop diseases and insect pests, and preparation method thereof - Google Patents
Composite microbial fertilizer for controlling crop diseases and insect pests, and preparation method thereof Download PDFInfo
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- CN101928186A CN101928186A CN2010101159286A CN201010115928A CN101928186A CN 101928186 A CN101928186 A CN 101928186A CN 2010101159286 A CN2010101159286 A CN 2010101159286A CN 201010115928 A CN201010115928 A CN 201010115928A CN 101928186 A CN101928186 A CN 101928186A
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 96
- 239000002131 composite material Substances 0.000 title claims abstract description 85
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 50
- 201000010099 disease Diseases 0.000 title claims abstract description 50
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 50
- 241000238631 Hexapoda Species 0.000 title claims abstract description 49
- 238000002360 preparation method Methods 0.000 title claims description 58
- 230000000813 microbial effect Effects 0.000 title abstract description 28
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 46
- 241000193388 Bacillus thuringiensis Species 0.000 claims abstract description 32
- 210000003608 fece Anatomy 0.000 claims abstract description 26
- 239000010871 livestock manure Substances 0.000 claims abstract description 26
- 229940097012 bacillus thuringiensis Drugs 0.000 claims abstract description 25
- 230000000749 insecticidal effect Effects 0.000 claims abstract description 24
- 238000002156 mixing Methods 0.000 claims abstract description 24
- 239000005648 plant growth regulator Substances 0.000 claims abstract description 23
- 239000000575 pesticide Substances 0.000 claims abstract description 17
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 16
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 15
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 14
- 241000287828 Gallus gallus Species 0.000 claims abstract description 14
- 230000000855 fungicidal effect Effects 0.000 claims abstract description 12
- 239000000417 fungicide Substances 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 10
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 9
- 239000002068 microbial inoculum Substances 0.000 claims description 86
- 230000002906 microbiologic effect Effects 0.000 claims description 72
- 244000005700 microbiome Species 0.000 claims description 49
- 238000000855 fermentation Methods 0.000 claims description 46
- 230000004151 fermentation Effects 0.000 claims description 46
- 239000003905 agrochemical Substances 0.000 claims description 36
- 238000000034 method Methods 0.000 claims description 36
- 239000000843 powder Substances 0.000 claims description 36
- 241000894006 Bacteria Species 0.000 claims description 29
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 27
- 229910052698 phosphorus Inorganic materials 0.000 claims description 27
- 239000011574 phosphorus Substances 0.000 claims description 27
- 238000006213 oxygenation reaction Methods 0.000 claims description 18
- 241000195940 Bryophyta Species 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 15
- 229930191978 Gibberellin Natural products 0.000 claims description 14
- 239000003448 gibberellin Substances 0.000 claims description 14
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims description 14
- 239000003375 plant hormone Substances 0.000 claims description 14
- 238000010521 absorption reaction Methods 0.000 claims description 13
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 12
- 239000011707 mineral Substances 0.000 claims description 12
- 235000010755 mineral Nutrition 0.000 claims description 12
- 239000003415 peat Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- UDPGUMQDCGORJQ-UHFFFAOYSA-N (2-chloroethyl)phosphonic acid Chemical compound OP(O)(=O)CCCl UDPGUMQDCGORJQ-UHFFFAOYSA-N 0.000 claims description 10
- FIPWRIJSWJWJAI-UHFFFAOYSA-N Butyl carbitol 6-propylpiperonyl ether Chemical compound C1=C(CCC)C(COCCOCCOCCCC)=CC2=C1OCO2 FIPWRIJSWJWJAI-UHFFFAOYSA-N 0.000 claims description 10
- 208000006558 Dental Calculus Diseases 0.000 claims description 10
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 10
- 229910021536 Zeolite Inorganic materials 0.000 claims description 10
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 claims description 10
- 229910021538 borax Inorganic materials 0.000 claims description 10
- 239000004202 carbamide Substances 0.000 claims description 10
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 10
- 239000011790 ferrous sulphate Substances 0.000 claims description 10
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 10
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 10
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 10
- 235000019837 monoammonium phosphate Nutrition 0.000 claims description 10
- 229960005235 piperonyl butoxide Drugs 0.000 claims description 10
- 239000004328 sodium tetraborate Substances 0.000 claims description 10
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 10
- 239000010457 zeolite Substances 0.000 claims description 10
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 claims description 9
- NOQGZXFMHARMLW-UHFFFAOYSA-N Daminozide Chemical compound CN(C)NC(=O)CCC(O)=O NOQGZXFMHARMLW-UHFFFAOYSA-N 0.000 claims description 9
- 239000005985 Paclobutrazol Substances 0.000 claims description 9
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 9
- 239000007921 spray Substances 0.000 claims description 9
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 9
- 229960001763 zinc sulfate Drugs 0.000 claims description 9
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 9
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 claims description 8
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 8
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 8
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 8
- 239000006012 monoammonium phosphate Substances 0.000 claims description 8
- 239000007864 aqueous solution Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
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- 108010080698 Peptones Proteins 0.000 claims description 5
- 239000004927 clay Substances 0.000 claims description 5
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- 238000012807 shake-flask culturing Methods 0.000 claims description 4
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- 244000068988 Glycine max Species 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 3
- 206010062575 Muscle contracture Diseases 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 3
- 208000006111 contracture Diseases 0.000 claims description 3
- 239000004467 fishmeal Substances 0.000 claims description 3
- 210000000582 semen Anatomy 0.000 claims description 3
- 235000013877 carbamide Nutrition 0.000 claims description 2
- 239000001963 growth medium Substances 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 19
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 7
- 229910052799 carbon Inorganic materials 0.000 abstract description 7
- 244000025254 Cannabis sativa Species 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 2
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- 239000011230 binding agent Substances 0.000 abstract 1
- VTIIJXUACCWYHX-UHFFFAOYSA-L disodium;carboxylatooxy carbonate Chemical compound [Na+].[Na+].[O-]C(=O)OOC([O-])=O VTIIJXUACCWYHX-UHFFFAOYSA-L 0.000 abstract 1
- 229940045872 sodium percarbonate Drugs 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 239000002689 soil Substances 0.000 description 12
- 230000003381 solubilizing effect Effects 0.000 description 12
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 11
- 239000011591 potassium Substances 0.000 description 11
- 229910052700 potassium Inorganic materials 0.000 description 11
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- 238000005516 engineering process Methods 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 8
- 229910019142 PO4 Inorganic materials 0.000 description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 230000012010 growth Effects 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 6
- 239000010452 phosphate Substances 0.000 description 6
- 235000021317 phosphate Nutrition 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241000726221 Gemma Species 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000000354 decomposition reaction Methods 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
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- 238000001228 spectrum Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
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- 238000012360 testing method Methods 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 244000105624 Arachis hypogaea Species 0.000 description 2
- 208000035143 Bacterial infection Diseases 0.000 description 2
- 241000255925 Diptera Species 0.000 description 2
- 241000242711 Fasciola hepatica Species 0.000 description 2
- 241000255777 Lepidoptera Species 0.000 description 2
- 241000255969 Pieris brassicae Species 0.000 description 2
- 239000002250 absorbent Substances 0.000 description 2
- 241001148470 aerobic bacillus Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 239000003899 bactericide agent Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
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- 210000000936 intestine Anatomy 0.000 description 2
- 231100000053 low toxicity Toxicity 0.000 description 2
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- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 235000020232 peanut Nutrition 0.000 description 2
- 239000002574 poison Substances 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000002594 sorbent Substances 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 244000144730 Amygdalus persica Species 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 244000241235 Citrullus lanatus Species 0.000 description 1
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 241001269238 Data Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
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- HIVLDXAAFGCOFU-UHFFFAOYSA-N ammonium hydrosulfide Chemical compound [NH4+].[SH-] HIVLDXAAFGCOFU-UHFFFAOYSA-N 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a composite microbial fertilizer for controlling crop diseases and insect pests, which contains composite microbial fungicide and is prepared by the following raw materials based on parts by weight: 10-35 parts of composite microbial fertilizer fungicide, 3-15 parts of composite microbial pesticide fungicide, 1-5 parts of insecticidal synergist, 1-5 parts of plant growth regulator, 20-60 parts of grass carbon, 5-15 parts of expanded chicken manure, 5-50 parts of inorganic fertilizer and 1-3 parts of binding agent, wherein the composite microbial fertilizer fungicide is prepared by bacillus megaterium and jelly-like bacillus; and the composite microbial pesticide fungicide is prepared by mixing bacillus thuringiensis and bacillus subtilis, adding sodium percarbonate type solid state oxygen-increasing agent for mixing and fermenting and finally absorbing by the grass carbon. The composite microbial fertilizer adopts strains with good suitability, and the strains are compounded according to a certain proportion to obtain the composite fungicide which has durable activity and stable performance; and the four beneficial strains are compatible with each other and performs the own functions, so that the multiple aims of resisting germs as well as diseases and insect pests, and increasing the crop yield can be achieved.
Description
Technical field
The invention belongs to the microbial fertilizer technical field, relate to a kind of microbial fertilizer and preparation method thereof with disease-resistant bacterium, disease and pest, increase crop yield three efficacy.
Background technology
The Development and Production green food, reduce the pollution and the harm that the mankind and environment are caused because of a large amount of use chemical fertilizer and chemical pesticide as far as possible, become one of problem of scientist's primary study, so microbial fertilizer and microbial pesticide arise at the historic moment, progressively develop into the comparatively important means of effectively preventing pollution, and be subjected to people's common attention day by day.
Normally a kind of composite fertilizer of microbial fertilizer, compound formulated by useful living microorganism flora, organic matter, inorganic fertilizer.Wherein Chang Yong living microorganism bacterium has vinelandii, phosphate solubilizing bacteria, potassium solubilizing bacteria etc.These useful living microorganism bacterium are to transform the indispensable active substance of soil fertility.Vinelandii can be fixed on the nitrogen in air and the soil plant roots and provide nitrogen nutrition for it, promote crop yield.Phosphate solubilizing bacteria, potassium solubilizing bacteria can resolve into elements such as the phosphorus of invalid attitude in the soil mineral, potassium the available phosphorus and the potassium that can directly be absorbed by crop.The living microorganism bacterium is participated in the conversion of soil material and energy, the formation and the processes such as decomposition and nutrient release of soil ulmin directly, for crop provides needed nutrient continuously.
In biological pesticide, study the most deep, most popular first-elected bacillus thuringiensis (Bacillus th uringiensis), it is a kind of naturally occurring entomiasis indigenous bacteria, is the normal component in the multiple soil, mainly is to make insect pathogenic dead by " stomach and intestine poison " effect.It can prevent and treat disease and pests such as more than 100 kind of lepidoptera pest, multiple Diptera pest and coleopteran pest, plant pathogeny line insect, mite, protozoon, liver fluke, it is only effective to the above-mentioned insect of getting food, and to the biological not influence of non-target, have the person poultry safety, insect is difficult for producing antibody, be easy to advantages such as suitability for industrialized production, provide condition for the mankind create non-harmful living environment.Through for many years, how geographic research on probation, prove its insect all to have good prevention effect to various crop such as harm fruit tree, vegetables, tealeaves, flowers.Sporeine preparation is the biotic pesticide of output maximum in the world today, the at present domestic also production technology and the technology of existing sophisticated thuringiensis cladosporioides bacillus insecticide, and this series products of how tame manufacturer production is arranged.
Subtilis (Bacillus subtilis) is a kind of microbial bactericide, it has extremely strong environmental compatibility, reproduction speed is fast, easily field planting is in the rhizosphere of crop, body surface, body or in the soil, by its competition effect that has, antagonistic action, bacteriolysis some bacterial diseases are produced the effect of control, the various bacterial disease that can be used for preventing and treating beans, wheat class, cotton, peanut, nursery stock, vegetables, fruit.
Vinelandii in the above-mentioned living microorganism microbial inoculum, phosphate solubilizing bacteria, potassium solubilizing bacteria normally use as the microbial fertilizer component; Above-mentioned bacillus thuringiensis, subtilis are used separately as microbial pesticide, sterilant.For vast user, should apply fertilizer satisfy the plant-growth needs, again need the medication deinsectization, kill germ, implementing operation respectively wastes time and energy, and production cost is higher.
Microbial pesticide, sterilant that existing independent employing bacillus thuringiensis, subtilis are prepared into are single culture, and fermentation separately.As with both mixing fermentation culture, also need growth rhythm according to microorganism species, carry out a large amount of suitability culture experiments, to determine conditional parameters such as the suitable temperature of fermentation, pressure.In addition, bacillus thuringiensis and subtilis are aerobic bacteria, and only in the competent environment of oxygen, they could good growth.Existing independent fermentation process though keep ventilation operation in the fermentation culture process, all can't guarantee the fermentation level of flora, and the quantity of flora and vigor are not high.
BecauseMicrobial fertilizer can not simply mix with microbial pesticide, sterilant
, thereforeExisting composite microbiological fertilizer, only has the single effect that increases crop yield or disease and insect resistance, for choosing of bacterial classification and other material, fail to consider the growth rhythm and the suitability of bacterial classification, and adopt traditional preparation technology can't make effects such as the increasing income of fertilizer, controlling crop diseases and insect pests reach best.
Summary of the invention
The invention provides composite microbiological fertilizer of a kind of controlling crop diseases and insect pests and preparation method thereof, be intended to using microbe fertilizer technology and microbial pesticide technology, adopt rational flora compounded technology method and effective operational path, prepare a kind of composite microbiological fertilizer with disease-resistant bacterium, disease and pest and increase crop yield three efficacy.Solve existing composite microbiological fertilizer function singleness, the bacterial classification suitability is not strong, the technical problem that effect of fertilizer is not good.
The technical solution adopted for the present invention to solve the technical problems is:
The composite microbiological fertilizer of this controlling crop diseases and insect pests contains complex micro organism fungicide, is cooperated by mass parts by following raw material to form:
10～35 parts of composite microbiological fertilizer microbial inoculums;
3～15 parts of complex microorganism agricultural chemicals microbial inoculums;
1～5 part of insecticidal synergist;
1～5 part of plant-growth regulator;
20～60 parts of the peats composed of rotten mosses;
5～15 parts of expanded chicken manures;
5～50 parts in mineral manure;
1～3 part in tackiness agent;
Described composite microbiological fertilizer microbial inoculum is formulated by following two kinds of microbial inoculums, and the per-cent that these two kinds of microbial inoculums account for composite microbiological fertilizer microbial inoculum gross weight is:
Bacillus megaterium Baeillus megatelium phosphaticum 40%～90%;
Bacillusmusilaginosiengineering Bacillus mucilginosus 10%～60%;
Described complex microorganism agricultural chemicals microbial inoculum is by bacillus thuringiensis Bacillus thuringiensis, two kinds of microbial inoculums of subtilis Bacillus subtilis are pressed arbitrary proportion and are mixed, after adding the solid-state oxygenation agent of SPC-D type mixed fermentation again, ratio absorption in the Fa Jiao Ye ︰ peat composed of rotten mosses=1 ﹕ 6 forms, and the addition of the solid-state oxygenation agent of SPC-D type is 0.025%～0.075 % of complex microorganism agricultural chemicals microbial inoculum gross weight in the mixed fermentation process.
Described insecticidal synergist is one or more the mixture in the agent of synergy phosphorus powder, Piperonyl Butoxide pulvis or the agent of methyl synergy phosphorus powder.
Described plant-growth regulator is that one or more the mixture in the pulvis is received by ethrel pulvis, alar-85 pulvis, paclobutrazol pulvis, Plant hormones regulators,gibberellins pulvis or IDALL.
Described mineral manure is one or more the mixture in urea, ammonium sulfate, monoammonium phosphate, vitriolate of tartar, borax, zinc sulfate, sal epsom, the ferrous sulfate, and it is as follows that various mineral manure account for the per-cent of composite microbiological fertilizer gross weight:
Urea 0～20%;
Ammonium sulfate 0～20%;
Monoammonium phosphate 0～15%;
Vitriolate of tartar 0～15%;
Borax 0～5%;
Zinc sulfate 0～3%;
Sal epsom 0～6%;
Ferrous sulfate 0～3%.
Described tackiness agent is clay, wilkinite or above-mentioned two kinds mixture.
The optimum mix that the raw material that is adopted reaches by mass parts is:
15 parts of composite microbiological fertilizer microbial inoculums;
5 parts of complex microorganism agricultural chemicals microbial inoculums;
35 parts of the peats composed of rotten mosses;
10 parts of expanded chicken manures;
3 parts in Plant hormones regulators,gibberellins pulvis;
2 parts of synergy phosphorus powder agent;
7 parts in urea;
10 parts in ammonium sulfate;
8 parts of monoammonium phosphates;
5 parts of vitriolate of tartar;
0.9 part of borax;
0.5 part in zinc sulfate;
1 part in ferrous sulfate;
0.6 part in sal epsom;
2 parts of clays.
Described composite microbiological fertilizer microbial inoculum is formulated by following two kinds of microbial inoculums, and the optimized percentage proportioning that these two kinds of microbial inoculums account for composite microbiological fertilizer microbial inoculum gross weight is:
Bacillus megaterium Baeillus megatelium phosphaticum 60%;
Bacillusmusilaginosiengineering Bacillus mucilginosus 40%;
Described complex microorganism agricultural chemicals microbial inoculum is by bacillus thuringiensis Bacillus thuringiensis, two kinds of microbial inoculums of subtilis Bacillus subtilis are by 1 ﹕, 1 proportioning, form with peat composed of rotten mosses absorption after adding the solid-state oxygenation agent of SPC-D type mixed fermentation again, the optimum addition of the solid-state oxygenation agent of SPC-D type is 0.04% of a complex microorganism agricultural chemicals microbial inoculum gross weight in the mixed fermentation process.
The preparation method of the composite microbiological fertilizer of this controlling crop diseases and insect pests, preparation process is as follows:
A, preparation composite microbiological fertilizer microbial inoculum
With bacillus megaterium pulvis and bacillusmusilaginosiengineering pulvis, press composite microbiological fertilizer microbial inoculum gross weight proportioning, make the composite microbiological fertilizer microbial inoculum after mixing, stirring, stand-by;
B, preparation complex microorganism agricultural chemicals microbial inoculum
After respectively bacillus thuringiensis, subtilis being carried out slant culture and shake-flask culture, cultured bacillus thuringiensis bacterium liquid and subtilis bacterium liquid changed in 1 ﹕, 1 ratio carry out mixing fermentation culture in the fermentor tank, the jar of fermentor tank holds 7t, charging capacity 60%, 30 ℃ ± 1 ℃ of culture temperature, operation tank pressure 0.3at, ventilating ratio 1 ﹕ 1.3, stir revolution 230r/min, fermentation period 24～26 hours; The fermentation beginning adds the solid-state oxygenation agent of SPC-D type after back 4 hours, added once every 2 hours later on, each addition 200g, after fermenting 20 hours, stop to add, the bacillus thuringiensis and the subtilis mixed fermentation liquid of preparation are adsorbed in the ratio of the Fa Jiao Ye ︰ peat composed of rotten mosses=1 ﹕ 6, make powdered complex microorganism pesticide powder, standby;
C, preparation insecticidal synergist pulvis
Insecticidal synergist synergy phosphorus, Piperonyl Butoxide or methyl synergy phosphorus are mixed with the aqueous solution that concentration is 500ppm respectively, part by weight by Shui Rong Ye ︰ zeolite powder=1 ︰ 10 sprays, stirs, fully promptly make the agent of synergy phosphorus powder, Piperonyl Butoxide pulvis, the agent of methyl synergy phosphorus powder respectively after the mixing absorption, standby;
D, preparation plant-growth regulator pulvis
Plant-growth regulator ethrel, alar-85, paclobutrazol, Plant hormones regulators,gibberellins, IDALL's contracture are not mixed with the aqueous solution that concentration is 800ppm, part by weight by Shui Rong Ye ︰ zeolite powder=1 ︰ 10 sprays, stirs, fully promptly make ethrel pulvis, alar-85 pulvis, paclobutrazol pulvis, Plant hormones regulators,gibberellins pulvis, IDALL's receipts pulvis after the mixing absorption respectively, standby;
The composite microbiological fertilizer of E, preparation controlling crop diseases and insect pests function
Will be by the composite microbiological fertilizer microbial inoculum of method A preparation, by the complex microorganism agricultural chemicals microbial inoculum of method B preparation, by method C prepare the insecticidal synergist pulvis, by the plant-growth regulator pulvis of method D preparation, with the peat composed of rotten mosses, expanded chicken manure, mineral manure, tackiness agent, get the raw materials ready by the proportioning metering;
F, will make the composite microbiological fertilizer of controlling crop diseases and insect pests function by the each component of said ratio metering through pulverizing, mixing, granulation, oven dry.
Among the described step B, when bacillus thuringiensis and subtilis carried out mixing fermentation culture, used culture medium prescription was: soybean cake powder 3.5%, Semen Maydis powder 2.5%, fish meal 5%, peptone 0.2%, yeast powder 1.5%, KH
2PO
40.7%, MgSO
40.06%, CaCO
30.3%, anti-crawl agentfoam oil 0.1%, and wherein the pulvis fineness is 140 orders, and substratum Ph is 7.4.
In the described step F; to there be the component of caking to pulverize earlier with pulverizer; and will mix by the each component of said ratio metering; be delivered to garden dish tablets press again; be processed into spherical particle; after being 50～70 ℃ convection type hot blast roller dryer drying through temperature out at last, sieve, making diameter is the composite microbiological fertilizer of the granular controlling crop diseases and insect pests function of 2～5mm.Beneficial effect of the present invention is as follows:
By the suitable proportion preparation, they are the complex micro organism fungicide that the present invention adopts: the powdery microbial inoculum of bacillus megaterium (Bacillus megatherium phosphaticum) powdery microbial inoculum, bacillusmusilaginosiengineering (Bacillus mucilginosus) powdery microbial inoculum, bacillus thuringiensis (Bacillus thuringiensis) and subtilis (Bacillus subtilis) mixing fermentation culture by following several powdery microbial inoculums.It has wide spectrum desinsection, kill germ and increase the three efficacy of output, more help improving the ability of crop disease-resistant bacterium, disease and pest, for saving the activity duration, reduce labour intensity, reduce production costs, prevent the pollution of the environment, development green ecological agricultural is of great practical significance.
Bacillus megaterium and bacillusmusilaginosiengineering are yield increasing funguses the most frequently used in the present microbial fertilizer, they not only have phosphorus decomposing, potassium decomposing with can not be by invalid phosphorus, the potassium of plant absorbing in the soil, decomposition and inversion is can be by the available phosphorus of plant absorbing and potassium, for plant provides abundant nutrition, in vital movements such as its fermentation, decomposition, can produce a large amount of Plant hormones regulators,gibberellins and chtokinin metabolic substd simultaneously, these metabolic substds are with after the root system of plant contacts, can stimulate the growth of crop, adjust and promote the metabolism of crop, produce effect of increasing production.In addition, the huge profitable strain (bacillus megaterium, bacillusmusilaginosiengineering, bacillus thuringiensis, subtilis) of quantity is bred in a large number, is grown at plant root, formed gemma class dominant microflora, suppress and reduced the breeding chance of harmful germ, played the effect that alleviates crop pest.
Bacillus thuringiensis is a kind of biotic pesticide of safety of wide spectrum, also is that use both at home and abroad is the most extensive at present, the microbial pesticide of output maximum.It is a kind of naturally occurring entomiasis indigenous bacteria, it is the normal component in the multiple soil, it can prevent and treat more than 100 kind of lepidoptera pest, multiple Diptera pest and coleopteran pest, plant pathogeny line insect, mite, protozoon, liver fluke etc., and it mainly is to make insect pathogenic dead by " stomach and intestine poison " effect.It is only effective to the above-mentioned insect of getting food, and to the biological not influence of non-target, has the person poultry safety, and insect is difficult for producing advantages such as antibody.
Subtilis (Bacillus subtilis) is a kind of microbial bactericide of low toxicity wide spectrum, and various crop bacteria type disease is had prevention and therapeutic action.But production technique is in the past carried out fermentation culture respectively with bacillus thuringiensis and subtilis, uses separately by the function that it had.The present invention carries out mixing fermentation culture with bacillus thuringiensis and subtilis, and this complex biological agricultural chemicals has desinsection strongly, kills the double effects of germ.Employing is soaked the Preliminary experiment results that the leaf method for breeding carries out the desinsection prevention effect of above-mentioned compound microbial agricultural chemicals and is shown (seeing Table 1), and the drug effect of the compound microbial agricultural chemicals of mixing fermentation culture obviously is better than the drug effect of the complex microorganism agricultural chemicals that independent fermentation culture remix makes.
Above-mentioned four kinds of microbial bacterias all belong to genus bacillus, i.e. phosphate solubilizing bacteria-bacillus megaterium; Potassium solubilizing bacteria--bacillusmusilaginosiengineering; Biotic pesticide--bacillus thuringiensis, biological bactericide-subtilis.And genus bacillus has two characteristics, the one, and widely distributed, adaptability is strong.The plant materials of the studying genus bacillus that all lived, have comparative advantage as doing object endospore bacillus in cotton etc.The 2nd, genus bacillus is big multipotency existence under the condition of aerobic or anaerobic, and opposing adverse circumstance ability is strong, can be rated as the hat in the microorganism.They can form the heavy-walled gemma, and heatproof, moisture-proof, anti-drying, the general bacterium of energy force rate alkaline-resisting, anti-ray want high 2--10 doubly, and present employed yield increasing fungus all is genus bacillus mostly.The optimum living environment temperature of above-mentioned four kinds of microbiobacterial agents is all about 28--32 ℃, optimum pH value all is 7～7.5, these basic datas are analyzed contrast, and carried out the suitability culture experiment, can tentatively draw such conclusion, be about to they composite by a certain percentage after, the number of viable of various microbial inoculums, activity are all very stable, suitability is good.This provides scientific basis and material conditions for preparing the composite microbiological fertilizer with controlling crop diseases and insect pests function.
The compound microbial agricultural chemicals of different process preparation is to the efficiency test of cabbage caterpillar
| The microbial inoculum code name | For examination borer population (only) | Borer population (only) alive behind the 48h | Borer population (only) alive behind the 72h | Average mortality (%) |
| 1#(0.5 hundred million spores/ml) | 20 | 4 | 1 | 95 |
| 2#(0.5 hundred million spores/ml) | 20 | 7 | 3 | 85 |
Wherein:
The 1# microbial inoculum is the compound microbial agricultural chemicals bacterium liquid of mixing fermentation culture, and wherein the bacillus thuringiensis working concentration is 0.5 hundred million spores/ml;
The 2# microbial inoculum is a decomposite microbial pesticide bacterium liquid after the independent fermentation culture, and wherein the bacillus thuringiensis working concentration is 0.5 hundred million spores/ml;
For raising insect: cabbage caterpillar
Feed: the Plantula Brassicae chinensis leaf, soak the back with 1#, 2# bacterium liquid respectively and take out, dry the back naturally and use.
The present invention has also used oxygenation agent novel process during the fermentation.Bacillus thuringiensis and subtilis are aerobic bacteria, and only in the competent environment of oxygen, they could good growth.In order to improve fermentation level, increase flora quantity and vigor, the present invention is except that the traditional ventilation operation of maintenance in whole fermentation culture process, also in zymotechnique, used the oxygenation agent, the oxygenation agent that the present invention adopts is the solid-state oxygenation agent of SPC-D type, and this product is a column, meets water and promptly emits oxygen, the oxygen level in the liquid that increases sharply, but the long period keep high dissolved oxygen state in the liquid.
According to the growth rhythm of microorganism species in the batch fermentation process, the oxygenation agent added in the fermentation beginning in back 4 hours, added once every 2 hours later on, and each addition 200g stops to add after fermenting 20 hours.This technology can guarantee microorganism cells in the interim acquisition sufficient dissolved oxygen of its logarithmic growth, high-speed growth, simultaneously also can keep fermenting enter stationary phase after the quick growth of gemma, obtain higher gemma rate of formation.The total viable count of bacterium liquid can reach 60～7,000,000,000/ml after the fermentation culture, improves yield about 10～15% than traditional technology.
The yield increasing fungus that carries out being adopted among complex microorganism agricultural chemicals that mixing fermentation culture makes and the present invention because of bacillus thuringiensis and subtilis is that phosphate solubilizing bacteria and potassium solubilizing bacteria suitability are good, the composite fungus agent of making after composite by a certain percentage is active lasting, stable performance, four kinds of probioticss are compatible, Each performs its own functions, finally reaches the multipurpose of disease-resistant bacterium, disease and pest and increase crop yield.
The present invention selects for use synergy phosphorus, Piperonyl Butoxide or methyl synergy phosphorus as insecticidal synergist.The no insecticidal action of this type of synergistic agent itself, but it can help the active constituent of sterilant to transmit in the plant body, and mixed-functional oxidase in the polypide that can suppress or weaken slows down medicament at its intravital decomposition and accretion rate, thereby increases drug effect.The insecticidal synergist that the present invention selects for use is made pulvis with zeolite powder as sorbent material, characteristics such as zeolite powder has porous, specific surface area is big, water-absorbent is strong, absorption property is good, it also can be improved the soil simultaneously, reaches slowly-releasing, long lasting purpose, is composite microbiological fertilizer synergy.
The present invention selects for use one or more of medicament of low toxicities such as ethrel, alar-85, paclobutrazol, Plant hormones regulators,gibberellins, IDALL's receipts, wide spectrum as plant-growth regulator, the becoming flower, result, come off and the maturation and the color and luster of fruit of the dormancy that they can regulate crop and sprouting, control crop.Characteristics such as the plant-growth regulator that the present invention selects for use is made pulvis with zeolite powder as sorbent material, and zeolite powder has porous, specific surface area is big, water-absorbent is strong, absorption property is good, it also can be improved the soil simultaneously, is composite microbiological fertilizer synergy.
The peat composed of rotten mosses that the present invention selects for use, expanded chicken manure are as the organic matter of fertilizer.
One or more mixtures of the urea that the present invention selects for use, sulphur ammonium, monoammonium phosphate, vitriolate of tartar, borax, zinc sulfate, sal epsom, ferrous sulfate are as the inanimate matter of fertilizer.
Clay that the present invention selects for use or bentonitic a kind of or mixture are as tackiness agent.
Composite microbiological fertilizer by above-mentioned component preparation with controlling crop diseases and insect pests function, contain yield increasing fungus (phosphate solubilizing bacteria, potassium solubilizing bacteria), biological pesticide (bacillus thuringiensis, subtilis), insecticidal synergist, plant-growth regulator, organic, multiple effective ingredient such as inorganic fertilizer, not only improved the effective nutrient content in the soil, the nutrition of the quick-acting and slowly-releasing of necessity also is provided for crop, also increased the ability of crop disease-resistant insect pest significantly, for vegetables, fruit tree, cotton, tealeaves, various crop such as flowers all have the effect of disease and insect resistance and volume increase, provide a kind of novel and effective fertilizer for producing green food.
The present invention has controlling crop diseases and insect pests and increases the composite microbiological fertilizer of output function, has carried out preliminary effect test in different areas such as Daxing, Beijing, Shouguang, Shandong, Laiwus respectively.Test-results shows, this composite microbiological fertilizer with controlling crop diseases and insect pests function can adopt seed dressing, make base manure, multiple fertilization mode such as topdress is used various crop such as cucumber, tomato, ginger, watermelon, peach, peanuts, all has to reduce disease and pest significantly, improve crop quality and increase the effect of output.Its concrete test-results is as shown in the table:
Composite microbiological fertilizer effect test with controlling crop diseases and insect pests function is table as a result
Embodiment
Embodiment 1
A, preparation composite microbiological fertilizer microbial inoculum
With bacillus megaterium pulvis and bacillusmusilaginosiengineering pulvis, press composite microbiological fertilizer microbial inoculum gross weight proportioning, make the composite microbiological fertilizer microbial inoculum after mixing, stirring, stand-by;
Described composite microbiological fertilizer microbial inoculum is formulated by following two kinds of microbial inoculums, and the per-cent that these two kinds of microbial inoculums account for composite microbiological fertilizer microbial inoculum gross weight is:
Bacillus megaterium Baeillus megatelium phosphaticum 60 %;
Bacillusmusilaginosiengineering Bacillus mucilginosus 40 %;
B, preparation complex microorganism agricultural chemicals microbial inoculum
Described complex microorganism agricultural chemicals microbial inoculum is by bacillus thuringiensis Bacillus thuringiensis, two kinds of microbial inoculums of subtilis Bacillus subtilis are pressed arbitrary proportion and are mixed, form with careless carbon absorption after adding the solid-state oxygenation agent of SPC-D type mixed fermentation, the optimized percentage that complex microorganism agricultural chemicals microbial inoculum accounts for the composite microbiological fertilizer gross weight of controlling crop diseases and insect pests is: 5%; The per-cent proportioning of the optimum addition of the solid-state oxygenation agent of SPC-D type is in the mixed fermentation process: be 0.04%.
Respectively bacillus thuringiensis, subtilis are carried out slant culture and shake-flask culture:
⑴, slant culture
Substratum: peptone 0.5%, extractum carnis 0.3%, NaCl 0.5%, agar 1.5%, MnSO
4H
2O 0.05%, and PH7.2 inserts the inclined-plane, 30 ℃ of constant temperature culture 72 hours.
⑵, shake-flask culture
Substratum: peptone 3%, yeast extract paste 0.1%, NaCl 0.1%, agar 1. 0%, MgSO
40.03%, KH
2PO
40.1%, PH7.5,200r/min, 30 ℃ of shaking tables were cultivated 10 hours.
⑶, mixing fermentation culture
Substratum: soybean cake powder 3.5%, Semen Maydis powder 2.5%, fish meal 5%, peptone 0.2%, yeast powder 1.5% KH
2PO
40.7%, MgSO
40.06%, CaCO
30.3%, anti-crawl agentfoam oil 0.1%, pulvis fineness 140 orders wherein, PH7.4.
Cultured bacillus thuringiensis bacterium liquid and subtilis bacterium liquid changed in 1 ﹕, 1 ratio carry out mixed fermentation in the fermentor tank, the jar of fermentor tank holds 7t, charging capacity 60%, 30 ℃ ± 1 ℃ of culture temperature, operation tank pressure 0.3at, ventilating ratio 1 ﹕ 1.3 stirs revolution 230r/min, fermentation period 24～26 hours; The fermentation beginning adds the solid-state oxygenation agent of SPC-D type after back 4 hours, added once every 2 hours later on, each addition 200g, after fermenting 20 hours, stop to add, the bacillus thuringiensis and the subtilis mixed fermentation liquid of preparation are adsorbed in the ratio of Fa Jiao Ye ︰ grass carbon=1 ﹕ 6, make powdered complex microorganism pesticide powder, standby;
C, preparation insecticidal synergist pulvis
Insecticidal synergist synergy phosphorus, Piperonyl Butoxide or methyl synergy phosphorus are mixed with the aqueous solution that concentration is 500ppm respectively, part by weight by Shui Rong Ye ︰ zeolite powder=1 ︰ 10 sprays, stirs, fully promptly make the agent of synergy phosphorus powder, Piperonyl Butoxide pulvis, the agent of methyl synergy phosphorus powder respectively after the mixing absorption, standby;
D, preparation plant-growth regulator pulvis
Plant-growth regulator ethrel, alar-85, paclobutrazol, Plant hormones regulators,gibberellins, IDALL's contracture are not mixed with the aqueous solution that concentration is 800ppm, part by weight by Shui Rong Ye ︰ zeolite powder=1 ︰ 10 sprays, stirs, fully promptly make ethrel pulvis, alar-85 pulvis, paclobutrazol pulvis, Plant hormones regulators,gibberellins pulvis, IDALL's receipts pulvis after the mixing absorption respectively, standby.
The composite microbiological fertilizer of E, preparation controlling crop diseases and insect pests function
Will be by the composite microbiological fertilizer microbial inoculum of method A preparation, by the complex microorganism agricultural chemicals microbial inoculum of method B preparation, by method C prepare the insecticidal synergist pulvis, by the plant-growth regulator pulvis of method D preparation, with the peat composed of rotten mosses, expanded chicken manure, mineral manure, tackiness agent, get the raw materials ready by following proportioning metering;
Composite microbiological fertilizer microbial inoculum 13.0%
Complex microorganism agricultural chemicals microbial inoculum 5.0%
Grass carbon 32.0%
Plant hormones regulators,gibberellins pulvis 3.0%
Synergy phosphorus powder agent 2.0%
Expanded chicken manure 10.0%
Urea 7.0%
Ammonium sulfate 10.0%
Monoammonium phosphate 8.0%
Vitriolate of tartar 5.0%
Borax 1.5%
Zinc sulfate 0.5%
Ferrous sulfate 0.7%
Sal epsom 0.3%
Clay 2.0%
After F, the component that caking will be arranged are pulverized with pulverizer; to mix by the each component of said ratio metering again; and then tablets press is coiled in the garden that is delivered to; evenly spray an amount of water during granulation; be processed into spherical particle; after being 50-70 ℃ convection type hot blast roller dryer drying through temperature out at last, sieve, promptly making diameter is the granular composite microbiological fertilizer with controlling crop diseases and insect pests function of 3-5mm.
Embodiment 2
A, preparation composite microbiological fertilizer microbial inoculum
Prepare complex micro organism fungicide according to method A among the embodiment 1, standby.
B, preparation complex microorganism agricultural chemicals microbial inoculum
Prepare complex microorganism agricultural chemicals microbial inoculum according to method B among the embodiment 1, standby.
C, preparation insecticidal synergist pulvis
Prepare the insecticidal synergist pulvis according to method C among the embodiment 1, standby.
D, preparation plant-growth regulator pulvis
Prepare the plant-growth regulator pulvis according to method D among the embodiment 1, standby.
The composite microbiological fertilizer that E, preparation have the controlling crop diseases and insect pests function
Will be by the composite microbiological fertilizer microbial inoculum of method A preparation, by the complex microorganism agricultural chemicals microbial inoculum of method B preparation, by method C prepare the insecticidal synergist pulvis, by the plant-growth regulator pulvis of method D preparation, with the peat composed of rotten mosses, expanded chicken manure, mineral manure, tackiness agent, get the raw materials ready by following proportioning metering;
Composite microbiological fertilizer microbial inoculum 15%
Complex microorganism agricultural chemicals microbial inoculum 4.5%
Grass carbon 30.0%
Plant hormones regulators,gibberellins pulvis 2.5%
Piperonyl Butoxide pulvis 1.0%
Expanded chicken manure 7.0%
Urea 10.0%
Ammonium sulfate 8.0%
Monoammonium phosphate 10.0%
Vitriolate of tartar 7.0%
Borax 1.0%
Ferrous sulfate 0.5%
Sal epsom 0.5%
Wilkinite 3.0%
After F, the component that earlier caking will be arranged are pulverized with pulverizer; to mix by the each component of said ratio metering again; and then tablets press is coiled in the garden that is delivered to; evenly spray an amount of water during granulation; be processed into spherical particle; after being 50-70 ℃ convection type hot blast roller dryer drying through temperature out at last, sieve, promptly making diameter is the granular composite microbiological fertilizer with controlling crop diseases and insect pests function of 3-5mm.
Embodiment 3
A, preparation composite microbiological fertilizer microbial inoculum
Prepare complex micro organism fungicide according to method A among the embodiment 1, standby.
B, preparation complex microorganism agricultural chemicals microbial inoculum
Prepare complex microorganism agricultural chemicals microbial inoculum according to method B among the embodiment 1, standby.
C, preparation insecticidal synergist pulvis
Prepare the insecticidal synergist pulvis according to method C among the embodiment 1, standby.
D, preparation plant-growth regulator pulvis
Prepare the plant-growth regulator pulvis according to method D among the embodiment 1, standby.
The composite microbiological fertilizer that E, preparation have the controlling crop diseases and insect pests function
Will be by the composite microbiological fertilizer microbial inoculum of method A preparation, by the complex microorganism agricultural chemicals microbial inoculum of method B preparation, by method C prepare the insecticidal synergist pulvis, by the plant-growth regulator pulvis of method D preparation, with the peat composed of rotten mosses, expanded chicken manure, mineral manure, tackiness agent, get the raw materials ready by following proportioning metering;
Composite microbiological fertilizer microbial inoculum 16%
Complex microorganism agricultural chemicals microbial inoculum 3.0%
Grass carbon 35.0%
Ethrel pulvis 3.0%
Synergy phosphorus powder agent 1.0%
Expanded chicken manure 6.0%
Urea 12.0%
Monoammonium phosphate 13.0%
Vitriolate of tartar 6.0%
Borax 0.8%
Zinc sulfate 0.6%
Ferrous sulfate 1.0%
Sal epsom 0.6%
Clay 2.0%
After F, the component that caking will be arranged are pulverized with pulverizer; to mix by the each component of said ratio metering again; and then tablets press is coiled in the garden that is delivered to; evenly spray an amount of water during granulation; be processed into spherical particle; after being 50-70 ℃ convection type hot blast roller dryer drying through temperature out at last, sieve, promptly making diameter is the granular composite microbiological fertilizer with controlling crop diseases and insect pests function of 2-5mm.
In complex micro organism fungicide, " strong garden board " microbiobacterial agent product that bacillus megaterium pulvis, bacillusmusilaginosiengineering pulvis all select for use the two Long Amusi company limiteds in Beijing to produce.
Claims (10)
1. the composite microbiological fertilizer of a controlling crop diseases and insect pests contains complex micro organism fungicide, it is characterized in that: cooperated by mass parts by following raw material to form:
10～35 parts of composite microbiological fertilizer microbial inoculums;
3～15 parts of complex microorganism agricultural chemicals microbial inoculums;
1～5 part of insecticidal synergist;
1～5 part of plant-growth regulator;
20～60 parts of the peats composed of rotten mosses;
5～15 parts of expanded chicken manures;
5～50 parts in mineral manure;
1～3 part in tackiness agent;
Described composite microbiological fertilizer microbial inoculum is formulated by following two kinds of microbial inoculums, and the per-cent that these two kinds of microbial inoculums account for composite microbiological fertilizer microbial inoculum gross weight is:
Bacillus megaterium Baeillus megatelium phosphaticum 40%～90%;
Bacillusmusilaginosiengineering Bacillus mucilginosus 10%～60%;
Described complex microorganism agricultural chemicals microbial inoculum is by bacillus thuringiensis Bacillus thuringiensis, two kinds of microbial inoculums of subtilis Bacillus subtilis are pressed arbitrary proportion and are mixed, after adding the solid-state oxygenation agent of SPC-D type mixed fermentation again, ratio absorption in the Fa Jiao Ye ︰ peat composed of rotten mosses=1 ﹕ 6 forms, and the addition of the solid-state oxygenation agent of SPC-D type is 0.025%～0.075 % of complex microorganism agricultural chemicals microbial inoculum gross weight in the mixed fermentation process.
2. the composite microbiological fertilizer of controlling crop diseases and insect pests according to claim 1 is characterized in that: described insecticidal synergist is one or more the mixture in the agent of synergy phosphorus powder, Piperonyl Butoxide pulvis or the agent of methyl synergy phosphorus powder.
3. the composite microbiological fertilizer of controlling crop diseases and insect pests according to claim 1 is characterized in that: described plant-growth regulator is that one or more the mixture in the pulvis is received by ethrel pulvis, alar-85 pulvis, paclobutrazol pulvis, Plant hormones regulators,gibberellins pulvis or IDALL.
4. the composite microbiological fertilizer of controlling crop diseases and insect pests according to claim 1, it is characterized in that: described mineral manure is one or more the mixture in urea, ammonium sulfate, monoammonium phosphate, vitriolate of tartar, borax, zinc sulfate, sal epsom, the ferrous sulfate, and it is as follows that various mineral manure account for the per-cent of composite microbiological fertilizer gross weight:
Urea 0～20%;
Ammonium sulfate 0～20%;
Monoammonium phosphate 0～15%;
Vitriolate of tartar 0～15%;
Borax 0～5%;
Zinc sulfate 0～3%;
Sal epsom 0～6%;
Ferrous sulfate 0～3%.
5. the composite microbiological fertilizer of controlling crop diseases and insect pests according to claim 1, it is characterized in that: described tackiness agent is clay, wilkinite or above-mentioned two kinds mixture.
6. the composite microbiological fertilizer of controlling crop diseases and insect pests according to claim 1 is characterized in that: the raw material that is adopted and be by the optimum mix of mass parts:
15 parts of composite microbiological fertilizer microbial inoculums;
5 parts of complex microorganism agricultural chemicals microbial inoculums;
35 parts of the peats composed of rotten mosses;
10 parts of expanded chicken manures;
3 parts in Plant hormones regulators,gibberellins pulvis;
2 parts of synergy phosphorus powder agent;
7 parts in urea;
10 parts in ammonium sulfate;
8 parts of monoammonium phosphates;
5 parts of vitriolate of tartar;
0.9 part of borax;
0.5 part in zinc sulfate;
1 part in ferrous sulfate;
0.6 part in sal epsom;
2 parts of clays.
7. the composite microbiological fertilizer of controlling crop diseases and insect pests according to claim 1, it is characterized in that: described composite microbiological fertilizer microbial inoculum is formulated by following two kinds of microbial inoculums, and the optimized percentage proportioning that these two kinds of microbial inoculums account for composite microbiological fertilizer microbial inoculum gross weight is:
Bacillus megaterium Baeillus megatelium phosphaticum 60%;
Bacillusmusilaginosiengineering Bacillus mucilginosus 40%;
Described complex microorganism agricultural chemicals microbial inoculum is by bacillus thuringiensis Bacillus thuringiensis, two kinds of microbial inoculums of subtilis Bacillus subtilis are by 1 ﹕, 1 proportioning, form with peat composed of rotten mosses absorption after adding the solid-state oxygenation agent of SPC-D type mixed fermentation again, the optimum addition of the solid-state oxygenation agent of SPC-D type is 0.04% of a complex microorganism agricultural chemicals microbial inoculum gross weight in the mixed fermentation process.
8. the preparation method of the composite microbiological fertilizer of described any one controlling crop diseases and insect pests of a claim 1～7 is characterized in that preparation process is as follows:
A, preparation composite microbiological fertilizer microbial inoculum
With bacillus megaterium pulvis and bacillusmusilaginosiengineering pulvis, press composite microbiological fertilizer microbial inoculum gross weight proportioning, make the composite microbiological fertilizer microbial inoculum after mixing, stirring, stand-by;
B, preparation complex microorganism agricultural chemicals microbial inoculum
After respectively bacillus thuringiensis, subtilis being carried out slant culture and shake-flask culture, cultured bacillus thuringiensis bacterium liquid and subtilis bacterium liquid changed in 1 ﹕, 1 ratio carry out mixing fermentation culture in the fermentor tank, the jar of fermentor tank holds 7t, charging capacity 60%, 30 ℃ ± 1 ℃ of culture temperature, operation tank pressure 0.3at, ventilating ratio 1 ﹕ 1.3, stir revolution 230r/min, fermentation period 24～26 hours; The fermentation beginning adds the solid-state oxygenation agent of SPC-D type after back 4 hours, added once every 2 hours later on, each addition 200g, after fermenting 20 hours, stop to add, the bacillus thuringiensis and the subtilis mixed fermentation liquid of preparation are adsorbed in the ratio of the Fa Jiao Ye ︰ peat composed of rotten mosses=1 ﹕ 6, make powdered complex microorganism pesticide powder, standby;
C, preparation insecticidal synergist pulvis
Insecticidal synergist synergy phosphorus, Piperonyl Butoxide or methyl synergy phosphorus are mixed with the aqueous solution that concentration is 500ppm respectively, part by weight by Shui Rong Ye ︰ zeolite powder=1 ︰ 10 sprays, stirs, fully promptly make the agent of synergy phosphorus powder, Piperonyl Butoxide pulvis, the agent of methyl synergy phosphorus powder respectively after the mixing absorption, standby;
D, preparation plant-growth regulator pulvis
Plant-growth regulator ethrel, alar-85, paclobutrazol, Plant hormones regulators,gibberellins, IDALL's contracture are not mixed with the aqueous solution that concentration is 800ppm, part by weight by Shui Rong Ye ︰ zeolite powder=1 ︰ 10 sprays, stirs, fully promptly make ethrel pulvis, alar-85 pulvis, paclobutrazol pulvis, Plant hormones regulators,gibberellins pulvis, IDALL's receipts pulvis after the mixing absorption respectively, standby.
The composite microbiological fertilizer of E, preparation controlling crop diseases and insect pests function
Will be by the composite microbiological fertilizer microbial inoculum of method A preparation, by the complex microorganism agricultural chemicals microbial inoculum of method B preparation, by method C prepare the insecticidal synergist pulvis, by the plant-growth regulator pulvis of method D preparation, with the peat composed of rotten mosses, expanded chicken manure, mineral manure, tackiness agent, get the raw materials ready by the proportioning metering;
F, will make the composite microbiological fertilizer of controlling crop diseases and insect pests function by the each component of said ratio metering through pulverizing, mixing, granulation, oven dry.
9. the preparation method of the composite microbiological fertilizer of controlling crop diseases and insect pests according to claim 8, it is characterized in that: among the described step B, when bacillus thuringiensis and subtilis carried out mixing fermentation culture, used culture medium prescription was: soybean cake powder 3.5%, Semen Maydis powder 2.5%, fish meal 5%, peptone 0.2%, yeast powder 1.5%, KH
2PO
40.7%, MgSO
40.06%, CaCO
30.3%, anti-crawl agentfoam oil 0.1%, and wherein the pulvis fineness is 140 orders, and substratum Ph is 7.4.
10. the preparation method of the composite microbiological fertilizer of controlling crop diseases and insect pests according to claim 8; it is characterized in that: in the described step F; to there be the component of caking to pulverize earlier with pulverizer; and will mix by the each component of said ratio metering; be delivered to garden dish tablets press again; be processed into spherical particle; after being 50～70 ℃ convection type hot blast roller dryer drying through temperature out at last; sieve, making diameter is the composite microbiological fertilizer of the granular controlling crop diseases and insect pests function of 2～5mm.
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| CN201010115928A CN101928186B (en) | 2010-03-02 | 2010-03-02 | Composite microbial fertilizer for controlling crop diseases and insect pests, and preparation method thereof |
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| CN101928186B CN101928186B (en) | 2012-10-24 |
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