CN103274825A - Special polyelement microorganism fertilizer for preventing celery soft rot and preparation method of special polyelement microorganism fertilizer - Google Patents
Special polyelement microorganism fertilizer for preventing celery soft rot and preparation method of special polyelement microorganism fertilizer Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 239000003337 fertilizer Substances 0.000 title abstract description 25
- 244000101724 Apium graveolens Dulce Group Species 0.000 title 1
- 240000007087 Apium graveolens Species 0.000 claims abstract description 41
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 38
- 241000894006 Bacteria Species 0.000 claims abstract description 31
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- 229940097012 bacillus thuringiensis Drugs 0.000 claims abstract description 23
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 19
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a special polyelement microorganism fertilizer for preventing celery soft rot and a preparation method of the special polyelement microorganism fertilizer. The special polyelement microorganism fertilizer for preventing the celery soft rot comprises components of raw materials in parts by weight as follows: 0.8-1 part of bacillus amyloliquefaciens, 0.6-0.8 part of bacillus thuringiensis, 0.5-0.7 part of bacillus subtilis, 0.4-0.8 part of lactic acid bacteria, 1-3 parts of a matrix leavening agent, 30-35 parts of maize straw, 20-30 parts of turf, Chinese herbal medicinal ingredients and the like. The prepared special polyelement microorganism fertilizer for preventing the celery soft rot can reduce the morbidity of the celery soft rot greatly, a plant can grow more vigorously, the yield of celery is increased greatly, the quality is improved, probiotics in the soil are also increased, the fertilizer is pollution-free and low in residue, and the soil condition is improved greatly.
Description
Technical field
The present invention relates to a kind of polynary microbial fertilizer, be specifically related to a kind of special-purpose fertilizer of polynary microorganism of preventing and treating the celery soft rot and preparation method thereof, belong to technical field of fertilizers.
Background technology
Celery is high fiber food, and its digestion in intestines produces the material of a kind of xylogen or intestines lactones, and this class material is a kind of antioxidant, often eat celery, especially eat leaf of Herba Apii graveolentis, all very useful to preventing hypertension, arteriosclerosis etc., and the assisting therapy effect is arranged.Contain compositions such as a large amount of fibers, potassium, Wei ShengsuB2, vitamin PP in the celery, these compositions can help us to relax bowel by human body, regulate sodium-potassium balance, and celery has a very important role to people in a word.
The celery soft rot claims " mashed pimple " again, belongs to bacterial disease, mainly betides on petiole base or the stem.
This disease early growth period after celery is transplanted seedling-slowing stage or slow seedling that mostly occurs.General elder generation begins morbidity from the petiole base of tender succulence.Their early stage, scab is filbert, water stain shape, fusiform or irregular shape, depression slightly, rapid expansion back interior tissue is chocolate and rots, foul smelling, residual epidermis only at last, whole celery lodging when serious of falling ill, therefore have a strong impact on quality and the output of celery, it is very important to prevent and treat the celery soft rot.
Feces of livestock and poultry behind crop material on-site incineration, the large-scale cultivations such as paddy rice, wheat, corn, the rape environment of having thrown aside etc. not only severe contamination everywhere in addition, also greatly having wasted can be as the raw material of fertilizer and biological organic fertilizer product; A large amount of nutrient resource (C, N, P, K, S and trace element) runs off outside soil-plant system, has weakened the ability of China's agricultural sustainable development significantly.Therefore taking full advantage of these resources also has very important meaning.
Control to the celery disease at present mainly utilizes agricultural chemicals, this method has been used a large amount of agricultural bactericides, a part is by the celery bulk absorption, can damage human body, and the vegetable grower is the general chemical fertilizer that adopts routine in the fertilising process, life-time service also has plant that certain influence is arranged to soil, so if solid organic castoffs such as stalk, ight soil are through the synthetic high-grade organic compost of thermophilic fermentation, be used as the carrier of function yeast again, made microorganism organic fertilizer definite functions will have good application prospects.At present the special-purpose fertilizer of earning for control celery soft rot of polynary microorganism be yet there are no report.
Summary of the invention
The present invention is directed to present situation, a kind of special-purpose fertilizer of polynary microorganism of preventing and treating the celery soft rot and preparation method thereof is provided, can effectively prevent the generation of celery soft rot, and improve its yield and quality, can also improve edaphic condition.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A kind of special-purpose fertilizer of polynary microorganism of preventing and treating the celery soft rot, its raw material comprises the composition of following weight ratio: bacillus amyloliquefaciens 0.8-1 part, Bacillus thuringiensis 0.6-0.8 part, subtilis 0.5-0.7 part, milk-acid bacteria 0.4-0.8 part, substrate fermentation agent 1-3 part, maize straw 30-35 part, peat composed of rotten mosses 20-30 part, flyash 30-36 part, mushroom slag 10-20 part, expanded pig manure 30-40 part, methyl synergy phosphorus powder agent 2-4 part, Piperonyl Butoxide pulvis 1-3 part, ethrel 0.4-0.6 part, urea 12-16 part, diammonium phosphate 10-12 part, vitriolate of tartar 6-8 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo kind skin 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, camphor 5-15 part, Semen Momordicae 5-15 part.
The special-purpose fertile preparation method of the polynary microorganism of above-mentioned control celery soft rot may further comprise the steps:
(1) multiplication culture of microorganism: bacillus amyloliquefaciens, Bacillus thuringiensis, subtilis, lactic bacterium strains are inoculated into respectively on the substratum, control the condition of fermentation separately well, final bacteria containing amount or gemma amount 〉=6 * 10 separately
9Individual/ml, make bacterium liquid;
(2) Bacillus thuringiensis, subtilis, lactic acid bacterial liquid and pig manure are carried out solid fermentation, turning every day is 1 time in the solid fermentation process, make the solid fermentation temperature be no more than 50 ℃, finish through fermentation in 6-8 days, Bacillus thuringiensis, subtilis, milk-acid bacteria continue to expand numerous simultaneously, obtain Bacillus thuringiensis microbial inoculum, bacillus subtilis microbial agent, milk-acid bacteria microbial inoculum, and the content of various microbial inoculums reaches 5 * 10
9Individual/more than the g;
(3) join the substrate fermentation agent in the mixture of maize straw, the peat composed of rotten mosses, flyash, mushroom slag according to described ratio, throwing is turned in mechanize, the timed interval be 1-2 days once, control temperature well, the solid fermentation temperature is no more than 60 ℃, when finding that material is loose, do not have the original stink of material, and the ammonia flavor is arranged slightly, fermentation finishes when producing white hypha in the heap, general process obtained the fermented product that moisture content is 35-40% in 8-10 days, and the microorganism in the substrate fermentation agent obtains expanding numerous simultaneously;
(4) material that step (2) and step (3) are made mixes, and after-ripening 2-3 days, in the after-ripening process turning every day once, turning is 2-4 time altogether, the last water content that is no more than in temperature under 60 ℃ the condition microbial organic fertilizer is evaporated to below 30%;
(5) urea, diammonium phosphate, vitriolate of tartar, the agent of methyl synergy phosphorus powder, Piperonyl Butoxide pulvis, ethrel, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo kind skin 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, camphor 5-15 part, Semen Momordicae 5-15 part is even according to described mixed; And then mix with material that step (4) makes, stir;
(6) dry, pulverize, make finished product: above-mentioned material is dried through fluidized-bed, and temperature control is at 90 ℃ below the degree, and water content is controlled in the 10-15% scope, crushing screening, and fineness reaches 80 orders; Send into the cylinder tablets press after crushed with travelling belt then, uperize makes material be heated to 50-60 ℃ in cylinder, moves by not stall of cylinder and makes the material balling-up; Then drying roller is sent in balling-up, the input hot-air seasoning cools off, screening, canned one-tenth finished product again.
The fermention medium proportioning of described bacillus amyloliquefaciens: 1% potato starch, 0.6%NaCl, 0.6% peptone, 0.3%KH
2PO
4Fermentation condition is: the fermentation flask of 60ml, and charging capacity 60%, temperature are 35-38 ℃, ventilating ratio 1:1.3, shaking speed is at 180r/min, and PH is 7-7.2, fermentation period 24-26 hour.
The fermention medium of described solution Bacillus thuringiensis is the LB liquid nutrient medium, and its proportioning is peptone 0.6g, yeast extract 0.27g, and sodium-chlor 0.6g adds water 50ml.Fermentation condition is: the fermentation flask of 60ml, temperature are 30-32 ℃, ventilating ratio 1:1.4, and shaking speed is at 220r/min, and PH is 7.5-8.5, fermentation period 28-30 hour.
The fermention medium improvement extractum carnis nutrient solution of described subtilis, its proportioning is: Semen Maydis powder 4g, extractum carnis 2g, sodium-chlor 4g, wheat bran 5g, potato starch 4g is settled to 800 ml, and the pH value transfers to 7.2-7.4,121 ℃ of sterilization 20 min.Fermentation condition is: the fermentation flask of 1000ml, temperature are 30-32 ℃, ventilating ratio 1:1.3, and shaking speed is at 240r/min, and PH is 7.2-7.4, fermentation period 22-24 hour.
The fermention medium of described milk-acid bacteria is the MRS substratum, and its proportioning is: 1% peptone, 2% beef extract powder, 0.5% yeast soak powder, 2% glucose, 0.2% diammonium hydrogen citrate, 0.1% tween 80,0.4% sodium acetate, 0.2% dipotassium hydrogen phosphate, 0.06% sal epsom, 0.02% manganous sulfate, 2% agar.Culture condition is: the fermentation flask of 500ml, temperature are 30-32 ℃, ventilating ratio 1:1.2, and shaking speed is at 240r/min, and PH is 6.2-6.4, fermentation period 26-28 hour.
Described bacillus amyloliquefaciens, Bacillus thuringiensis, subtilis, lactic bacterium strains are produced by the logical growth Science and Technology Ltd. in Shanghai; The substrate fermentation agent is produced by the positive agricultural agricultural science and technology in Beijing company limited;
The invention has the beneficial effects as follows:
(1) bacillus amyloliquefaciens has certain antagonistic action to the soft rot of celery, can effectively suppress the generation of soft rot.Energy formation advantage group after microorganism species such as the Bacillus thuringiensis that contains in the fertilizer, subtilis, milk-acid bacteria are manured into soil, and in soil, continue to expand numerous under the survival.
(2) selected substrate fermentation agent includes ten multiple beneficial microorganisms such as fungi, yeast, actinomycetes and bacterium, adopt modern advanced biotechnology make each flora of its microorganism can harmonious coexistence, mutually promote, reach the effect of mutual supplement with each other's advantages.And contained probiotics is bred fast in the substrate fermentation process and is produced macrometabolic element, can play and prevent and treat soil-borne disease, promotes the effect of plant growth.
(3) test-results shows, after the soil of plantation celery is used this product, can significantly fall the sickness rate of soft rot, and the biological control rate reaches more than 75%.
(4) of the present inventionly can be used for broadcasting soil treatment before the seedling of back, prevent and kill off the sterilization idiocratic of farmland weed and wide spectrum.Compared with prior art, it is good, environmentally safe that distinctive prescription synergy formulation of the present invention has a herbicidal effect, and weeds characteristics such as be difficult for developing immunity to drugs have a extensive future.Bactericidal and herbicidal agent of the present invention relatively safely to eyes, skin nonirritant, is low toxicity through bright its acute toxicity of day skin test checking to people and animals, easily decomposes in atmosphere and soil, and non-poisonous material is residual; The present invention's seed dressing is little to the influence of farmland natural enemy, helps to keep ecotope, and is more friendly to environment, satisfies the production requirement of crop.
Embodiment
Below preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that protection scope of the present invention is made more explicit defining.
Embodiment 1
A kind of special-purpose fertilizer of polynary microorganism of preventing and treating the celery soft rot, its raw material comprises the composition of following weight ratio: 0.8 part of bacillus amyloliquefaciens, 0.6 part of Bacillus thuringiensis, 0.5 part of subtilis, 0.4 part of milk-acid bacteria, 1 part of substrate fermentation agent, 30 parts of maize straws, 20 parts of the peats composed of rotten mosses, 30 parts in flyash, 10 parts of mushroom slags, 30 parts of expanded pig manures, 2 parts of methyl synergy phosphorus powder agent, 1 part in Piperonyl Butoxide pulvis, 0.4 part of ethrel, 12 parts in urea, 10 parts of diammonium phosphate, 6 parts of vitriolate of tartar, 5 parts of matrines, 10 parts of Golden Larch Barks, 20 parts in Chinese prickly ash, 20 parts of ginkgo kind skins, 20 parts of Fructus Cnidiis, 10 parts of Cortex Meliaees, 15 parts of Rangooncreeper Fruit, 1 part in Turkey-galls, 5 parts of Root of Pubescent Holly, 55 parts of Seed of Chinese Soapberry exospers, 10 parts of fructus ulmis, 5 parts of camphors, 5 parts of Semen Momordicaes.
The special-purpose fertile preparation method of the polynary microorganism of above-mentioned control celery soft rot may further comprise the steps:
(1) multiplication culture of microorganism: bacillus amyloliquefaciens, Bacillus thuringiensis, subtilis, lactic bacterium strains are inoculated into respectively on the substratum, control the condition of fermentation separately well, final bacteria containing amount or gemma amount 〉=6 * 10 separately
9Individual/ml, make bacterium liquid;
(2) Bacillus thuringiensis, subtilis, lactic acid bacterial liquid and pig manure are carried out solid fermentation, turning every day is 1 time in the solid fermentation process, make the solid fermentation temperature be no more than 50 ℃, finish through fermentation in 6-8 days, Bacillus thuringiensis, subtilis, milk-acid bacteria continue to expand numerous simultaneously, obtain Bacillus thuringiensis microbial inoculum, bacillus subtilis microbial agent, milk-acid bacteria microbial inoculum, and the content of various microbial inoculums reaches 5 * 10
9Individual/more than the g;
(3) join the substrate fermentation agent in the mixture of maize straw, the peat composed of rotten mosses, flyash, mushroom slag according to described ratio, throwing is turned in mechanize, the timed interval be 1-2 days once, control temperature well, the solid fermentation temperature is no more than 60 ℃, when finding that material is loose, do not have the original stink of material, and the ammonia flavor is arranged slightly, fermentation finishes when producing white hypha in the heap, general process obtained the fermented product that moisture content is 35-40% in 8-10 days, and the microorganism in the substrate fermentation agent obtains expanding numerous simultaneously;
(4) material that step (2) and step (3) are made mixes, and after-ripening 2-3 days, in the after-ripening process turning every day once, turning is 2-4 time altogether, the last water content that is no more than in temperature under 60 ℃ the condition microbial organic fertilizer is evaporated to below 30%;
(5) urea, diammonium phosphate, vitriolate of tartar, the agent of methyl synergy phosphorus powder, Piperonyl Butoxide pulvis, ethrel, 5 parts of matrines, 10 parts of Golden Larch Barks, 20 parts in Chinese prickly ash, 20 parts of ginkgo kind skins, 20 parts of Fructus Cnidiis, 10 parts of Cortex Meliaees, 15 parts of Rangooncreeper Fruit, 1 part in Turkey-galls, 5 parts of Root of Pubescent Holly, 55 parts of Seed of Chinese Soapberry exospers, 10 parts of fructus ulmis, 5 parts of camphors, 5 parts of Semen Momordicaes is even according to described mixed, add water; And then mix with material that step (4) makes, stir;
(6) dry, pulverize, make finished product: above-mentioned material is dried through fluidized-bed, and temperature control is at 90 ℃ below the degree, and water content is controlled in the 10-15% scope, crushing screening, and fineness reaches 80 orders; Send into the cylinder tablets press after crushed with travelling belt then, uperize makes material be heated to 50-60 ℃ in cylinder, moves by not stall of cylinder and makes the material balling-up; Then drying roller is sent in balling-up, the input hot-air seasoning cools off, screening, canned one-tenth finished product again.
The fermention medium proportioning of described bacillus amyloliquefaciens: 1% potato starch, 0.6%NaCl, 0.6% peptone, 0.3%KH
2PO
4Fermentation condition is: the fermentation flask of 60ml, and charging capacity 60%, temperature are 35-38 ℃, ventilating ratio 1:1.3, shaking speed is at 180r/min, and PH is 7-7.2, fermentation period 24-26 hour.
The fermention medium of described solution Bacillus thuringiensis is the LB liquid nutrient medium, and its proportioning is peptone 0.6g, yeast extract 0.27g, and sodium-chlor 0.6g adds water 50ml.Fermentation condition is: the fermentation flask of 60ml, temperature are 30-32 ℃, ventilating ratio 1:1.4, and shaking speed is at 220r/min, and PH is 7.5-8.5, fermentation period 28-30 hour.
The fermention medium improvement extractum carnis nutrient solution of described subtilis, its proportioning is: Semen Maydis powder 4g, extractum carnis 2g, sodium-chlor 4g, wheat bran 5g, potato starch 4g is settled to 800 ml, and the pH value transfers to 7.2-7.4,121 ℃ of sterilization 20 min.Fermentation condition is: the fermentation flask of 1000ml, temperature are 30-32 ℃, ventilating ratio 1:1.3, and shaking speed is at 240r/min, and PH is 7.2-7.4, fermentation period 22-24 hour.
The fermention medium of described milk-acid bacteria is the MRS substratum, and its proportioning is: 1% peptone, 2% beef extract powder, 0.5% yeast soak powder, 2% glucose, 0.2% diammonium hydrogen citrate, 0.1% tween 80,0.4% sodium acetate, 0.2% dipotassium hydrogen phosphate, 0.06% sal epsom, 0.02% manganous sulfate, 2% agar.Culture condition is: the fermentation flask of 500ml, temperature are 30-32 ℃, ventilating ratio 1:1.2, and shaking speed is at 240r/min, and PH is 6.2-6.4, fermentation period 26-28 hour.
Described substrate fermentation agent is produced by the professional research and development of the positive agricultural agricultural science and technology company limited in Beijing, living bacteria count 〉=200,000,000/g is specifically designed to processing by composite organic substrates that forms of various waste materials such as feces of livestock and poultry, agricultural crop straw, slag, river sand, flyash, mushroom slags.
Embodiment 2
A kind of polynary microorganism that prevents and treats the celery soft rot is special-purpose fertile, comprises the composition of following weight ratio: 0.9 part of bacillus amyloliquefaciens, 0.7 part of Bacillus thuringiensis, 0.6 part of subtilis, 0.6 part of milk-acid bacteria, 2 parts of substrate fermentation agent, 32 parts of maize straws, 25 parts of the peats composed of rotten mosses, 33 parts in flyash, 15 parts of mushroom slags, 35 parts of expanded pig manures, 3 parts of methyl synergy phosphorus powder agent, 2 parts in Piperonyl Butoxide pulvis, 0.5 part of ethrel, 14 parts in urea, 11 parts of diammonium phosphate, 7 parts of vitriolate of tartar, 5 parts of matrines, 10 parts of Golden Larch Barks, 20 parts in Chinese prickly ash, 20 parts of ginkgo kind skins, 20 parts of Fructus Cnidiis, 10 parts of Cortex Meliaees, 15 parts of Rangooncreeper Fruit, 1 part in Turkey-galls, 5 parts of Root of Pubescent Holly, 55 parts of Seed of Chinese Soapberry exospers, 10 parts of fructus ulmis, 5 parts of camphors, 5 parts of Semen Momordicaes.
The fermentation proliferated culture medium of preparation method and various bacterium and culture condition thereof are with embodiment 1.
Embodiment 3
A kind of special-purpose fertilizer of polynary microorganism of preventing and treating the celery soft rot, its raw material comprises the composition of following weight ratio: 1 part of bacillus amyloliquefaciens, 0.8 part of Bacillus thuringiensis, 0.7 part of subtilis, 0.8 part of milk-acid bacteria, 3 parts of substrate fermentation agent, 35 parts of maize straws, 30 parts of the peats composed of rotten mosses, 36 parts in flyash, 20 parts of mushroom slags, 40 parts of expanded pig manures, 4 parts of methyl synergy phosphorus powder agent, 3 parts in Piperonyl Butoxide pulvis, 0.6 part of ethrel, 16 parts in urea, 12 parts of diammonium phosphate, 8 parts of vitriolate of tartar, 5 parts of matrines, 10 parts of Golden Larch Barks, 20 parts in Chinese prickly ash, 20 parts of ginkgo kind skins, 20 parts of Fructus Cnidiis, 10 parts of Cortex Meliaees, 15 parts of Rangooncreeper Fruit, 1 part in Turkey-galls, 5 parts of Root of Pubescent Holly, 55 parts of Seed of Chinese Soapberry exospers, 10 parts of fructus ulmis, 5 parts of camphors, 5 parts of Semen Momordicaes.
The fermentation proliferated culture medium of preparation method and various bacterium and culture condition thereof are with embodiment 1.
In Ma Jiagou celery planting base, Qingdao, two processing are set, each handles three repetitions, each residential quarter 80m
2, celery is unified plantation, outdoor cropping, unified management; The polynary fertilizer of using polynary microorganism special use fertilizer of the present invention and the production of the fertile industry of Qufu City Jin Shili science and technology company limited with 50kg/ mu usage quantity respectively (contrasts 1, its total nutrient 〉=45%, N-P-K is 28-6-6), in vegetative period it is carried out field investigation, situations such as the growth of investigation celery and soft rot generation, and its output is surveyed in the results back, and comparative result sees Table 1:
The polynary microbial fertilizer of table 1 the present invention preparation and contrast 1 are relatively
 |
Soft rot reduction (%) | Output increment (%) | Soil probiotics increment (%) |
| Embodiment 1 | 66 | 18 | 33 |
| Embodiment 2 | 75 | 22 | 40 |
| Embodiment 3 | 70 | 20 | 36 |
The result shows, the special-purpose fertilizer of the polynary microorganism of the control celery soft rot of the present invention's preparation can make the soft rot sickness rate of celery reduce greatly, plant strain growth is more vigorous, the output of celery is greatly enhanced, quality also improves, probiotic bacterium in the soil also increases, and the situation of soil is greatly improved.Of the present invention through the field simultaneous test, all obtain positive effect, mainly show as follows: contain and enrich organic matter and humic acid, can improve Soil structure, vegetable soil promotes soil to build up fertility.With other fertilizer ratio such as nutrient such as grade, crop yield.Formulation and the nutrition of coordination crop absorption stimulate and the regulation and control plant growth, reduce or the reduction crop pest, influence crop quality.Need not or reduce fertilizer amount, be beneficial to the protection environment.
The above only is embodiments of the invention; be not so limit claim of the present invention; every equivalent structure or equivalent flow process conversion that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the present invention.
Claims (10)
1. a polynary microorganism that prevents and treats the celery soft rot is special-purpose fertile, it is characterized in that its raw material comprises the composition of following weight ratio: bacillus amyloliquefaciens 0.8-1 part, Bacillus thuringiensis 0.6-0.8 part, subtilis 0.5-0.7 part, milk-acid bacteria 0.4-0.8 part, substrate fermentation agent 1-3 part, maize straw 30-35 part, peat composed of rotten mosses 20-30 part, flyash 30-36 part, mushroom slag 10-20 part, expanded pig manure 30-40 part, methyl synergy phosphorus powder agent 2-4 part, Piperonyl Butoxide pulvis 1-3 part, ethrel 0.4-0.6 part, urea 12-16 part, diammonium phosphate 10-12 part, vitriolate of tartar 6-8 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo kind skin 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, camphor 5-15 part, Semen Momordicae 5-15 part.
2. a kind of special-purpose fertile preparation method of polynary microorganism who prevents and treats the celery soft rot according to claim 1 is characterized in that, may further comprise the steps:
(1) multiplication culture of microorganism: bacillus amyloliquefaciens, Bacillus thuringiensis, subtilis, lactic bacterium strains are inoculated into respectively on the substratum, control the condition of fermentation separately well, final bacteria containing amount or gemma amount 〉=6 * 10 separately
9Individual/ml, make bacterium liquid;
(2) Bacillus thuringiensis, subtilis, lactic acid bacterial liquid and pig manure are carried out solid fermentation, turning every day is 1 time in the solid fermentation process, make the solid fermentation temperature be no more than 50 ℃, finish through fermentation in 6-8 days, Bacillus thuringiensis, subtilis, milk-acid bacteria continue to expand numerous simultaneously, obtain Bacillus thuringiensis microbial inoculum, bacillus subtilis microbial agent, milk-acid bacteria microbial inoculum, and the content of various microbial inoculums reaches 5 * 10
9Individual/more than the g;
(3) join the substrate fermentation agent in the mixture of maize straw, the peat composed of rotten mosses, flyash, mushroom slag according to described ratio, throwing is turned in mechanize, the timed interval be 1-2 days once, control temperature well, the solid fermentation temperature is no more than 60 ℃, when finding that material is loose, do not have the original stink of material, and the ammonia flavor is arranged slightly, fermentation finishes when producing white hypha in the heap, general process obtained the fermented product that moisture content is 35-40% in 8-10 days, and the microorganism in the substrate fermentation agent obtains expanding numerous simultaneously;
(4) material that step (2) and step (3) are made mixes, and after-ripening 2-3 days, in the after-ripening process turning every day once, turning is 2-4 time altogether, the last water content that is no more than in temperature under 60 ℃ the condition microbial organic fertilizer is evaporated to below 30%;
(5) urea, diammonium phosphate, vitriolate of tartar, the agent of methyl synergy phosphorus powder, Piperonyl Butoxide pulvis, ethrel, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo kind skin 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, camphor 5-15 part, Semen Momordicae 5-15 part is even according to described mixed; And then mix with material that step (4) makes, stir;
(6) dry, pulverize, make finished product: above-mentioned material is dried through fluidized-bed, and temperature control is at 90 ℃ below the degree, and water content is controlled in the 10-15% scope, crushing screening, and fineness reaches 80 orders; Send into the cylinder tablets press after crushed with travelling belt then, uperize makes material be heated to 50-60 ℃ in cylinder, moves by not stall of cylinder and makes the material balling-up; Then drying roller is sent in balling-up, the input hot-air seasoning cools off, screening, canned one-tenth finished product again.
3. a kind of special-purpose fertile preparation method of polynary microorganism who prevents and treats the celery soft rot according to claim 2, in the step (1), the fermention medium proportioning of described bacillus amyloliquefaciens: 1% potato starch, 0.6%NaCl, 0.6% peptone, 0.3%KH
2PO
4
4. fermentation condition is: the fermentation flask of 60ml, and charging capacity 60%, temperature are 35-38 ℃, ventilating ratio 1:1.3, shaking speed is at 180r/min, and PH is 7-7.2, fermentation period 24-26 hour.
5. a kind of special-purpose fertile preparation method of polynary microorganism who prevents and treats the celery soft rot according to claim 2, in the step (1), the fermention medium of described solution Bacillus thuringiensis is the LB liquid nutrient medium, its proportioning is peptone 0.6g, yeast extract 0.27g, sodium-chlor 0.6g adds water 50ml.
6. fermentation condition is: the fermentation flask of 60ml, temperature are 30-32 ℃, ventilating ratio 1:1.4, and shaking speed is at 220r/min, and PH is 7.5-8.5, fermentation period 28-30 hour.
7. a kind of special-purpose fertile preparation method of polynary microorganism who prevents and treats the celery soft rot according to claim 2, in the step (1), the fermention medium improvement extractum carnis nutrient solution of described subtilis, its proportioning is: Semen Maydis powder 4g, extractum carnis 2g, sodium-chlor 4g, wheat bran 5g, potato starch 4g is settled to 800 ml, the pH value transfers to 7.2-7.4,121 ℃ of sterilization 20 min.
8. fermentation condition is: the fermentation flask of 1000ml, temperature are 30-32 ℃, ventilating ratio 1:1.3, and shaking speed is at 240r/min, and PH is 7.2-7.4, fermentation period 22-24 hour.
9. a kind of special-purpose fertile preparation method of polynary microorganism who prevents and treats the celery soft rot according to claim 2, in the step (1), the fermention medium of described milk-acid bacteria is the MRS substratum, and its proportioning is: 1% peptone, 2% beef extract powder, 0.5% yeast soak powder, 2% glucose, 0.2% diammonium hydrogen citrate, 0.1% tween 80,0.4% sodium acetate, 0.2% dipotassium hydrogen phosphate, 0.06% sal epsom, 0.02% manganous sulfate, 2% agar.
10. culture condition is: the fermentation flask of 500ml, temperature are 30-32 ℃, ventilating ratio 1:1.2, and shaking speed is at 240r/min, and PH is 6.2-6.4, fermentation period 26-28 hour.
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