CN104351472A - Bacillus subtilis fermentation broth of Chinese herbal medicine as well as preparation and application of bacillus subtilis fermentation broth - Google Patents
Bacillus subtilis fermentation broth of Chinese herbal medicine as well as preparation and application of bacillus subtilis fermentation broth Download PDFInfo
- Publication number
- CN104351472A CN104351472A CN201410537587.XA CN201410537587A CN104351472A CN 104351472 A CN104351472 A CN 104351472A CN 201410537587 A CN201410537587 A CN 201410537587A CN 104351472 A CN104351472 A CN 104351472A
- Authority
- CN
- China
- Prior art keywords
- bacillus subtilis
- fermentation
- herbal medicine
- liquid
- chinese herbal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to bacillus subtilis fermentation broth of a Chinese herbal medicine as well as preparation and an application of the bacillus subtilis fermentation broth. The Chinese herbal medicine refers to Chinese gall; according to pasteurella multocida and staphylococcus aureus inhibiting screening tests, the Chinese gall can inhibit growth of the pasteurella multocida and the staphylococcus aureus, bacillus subtilis AP139 is used for fermenting the Chinese gall, spraying is performed in an animal breeding shed, the fermented Chinese herbal medicine remarkably inhibits the growth of the pasteurella multocida and the staphylococcus aureus in the air, the effect is superior to those of a bacillus subtilis AP139 separate fermentation group and a Chinese herbal medicine control group, and the fermentation broth has the broader application prospect.
Description
Technical field
The invention belongs to fermentable bactericide field, be specifically related to gallic fermentation of bacillus subtilis liquid of a kind of Chinese herbal medicine and preparation method thereof, and it is for suppressing the application of pasteurella multocida and staphylococcus aureus.
Background technology
Pasteurella multocida, staphylococcus aureus are poultrys, fowl, wild animal and the mankind the important substance that suffers from venereal disease altogether of one, mainly through respiratory infectious.Pasteurella multocida can cause cholera fowl, swine plague and ox bleeding type septicemia etc., and toxigenic Pasteurella multocida also can cause atrophic rhinitis, and staphylococcus aureus can cause mastadenitis of cow, food pollution, wound suppuration etc.Staphylococcus aureus, pasteurella multocida spread all over air, water, people and animals' excreta, ubiquity pasteurella multocida and staphylococcus aureus in livestock and poultry farm, and not using, the antibiotic poultry house incidence of disease is higher.
Along with livestock and poultry cultivation is intensive and large-scale degree improves constantly, plant's air quality reduces gradually, and the probability that pasteurella multocida and staphylococcus aureus cause animal to fall ill and the extent of damage constantly increase.At present, plant controls to carry out prevention and therapy mainly through antibiotic to the disease that pasteurella multocida and staphylococcus aureus cause.But, antibiotic a large amount of use causes autogenous infection and suprainfection, the generation of bacterial resistance bacterial strain, immunity of livestock decline and residually in livestock products and environment cause a series of serious problems such as secondary pollution, and a lot of countries and regions such as European Union and Korea S all prohibit the use antibiotic.Therefore, how affecting human and environment under condition that is less or that do not affect, effectively preventing infection and the propagation of pasteurella multocida and staphylococcus aureus, is a urgent problem.
The Chinese herbal medicine fermentation of bacillus subtilis liquid of the present invention's development effectively can suppress the growth of pasteurella multocida and staphylococcus aureus in livestock and poultry farm, reduce livestock and poultry farm pasteurella multocida and the staphylococcus aureus incidence of disease, reduce the use of routine disinfection agent, the advantage such as have environmental protection, have no side effect, has a good application prospect in China's aquaculture is produced.
Summary of the invention
Main purpose of the present invention is to provide the gallic fermentation of bacillus subtilis liquid of a kind of Chinese herbal medicine and methods for making and using same thereof, this zymotic fluid can suppress the growth and breeding of pasteurella multocida in breeding house, staphylococcus aureus significantly, reduce the incidence of disease that plant is caused by pasteurella multocida, staphylococcus aureus, reduce livestock and poultry farm disinfectant and antibiotic use.
A kind of preparation method of fermentation of bacillus subtilis liquid of Chinese herbal medicine: be that bacillus subtilis (Bacillus subtilis) AP139 of CCTCC NO:M 2014053 and Chinese herbal medicine Chinese gall carry out liquid fermentation and culture and make by deposit number.
After said method fermented and cultured, bacillus subtilis bacterial content is 10
8cFU/ml-10
9cFU/ml.
In said method, Chinese gall adds mass ratio is the 1-5% of liquid fermentation medium.
The bacillus subtilis AP139 liquid medium that said method specifically will obtain through Shaking culture, according to liquid fermentation medium percent by volume 1-5% inoculum concentration, be inoculated in fermentation tank, liquid fermentation medium composition and mass percent are: bacteriological peptone 1%, sodium chloride 0.5%, glucose 1%, epsom salt 1%, gallnut extract 1-5%, pH value nature; Fermentation condition is: liquid amount percent by volume 40-70%, fermentation temperature 30-40 DEG C, pH value nature, fermentation time 24-36h, rotating speed, 200-400r/min.
First the bacillus subtilis AP139 of preservation is brought back to life before said method fermentation:
Bacillus subtilis AP139, on LB solid medium, carries out line and cultivates, cultivation temperature 30-37 DEG C, incubation time 18-24h in constant temperature biochemical cultivation case; LB solid culture based component and mass percent are: bacteriological peptone 1%, YE 0.5%, sodium chloride 0.5%, agar powder 1.5%, pH value 6.8-7.2.
Then from LB solid medium picking bring back to life the single clone strain of bacillus subtilis AP139 in 500 milliliters of conical flasks that 100 milliliters of LB fluid nutrient mediums are housed, cultivate in constant temperature biochemical rocking machine, obtain bacillus subtilis AP139 liquid medium, cultivation temperature 30-37 DEG C, rotating speed 180-220r/min, incubation time 18-24h, LB Liquid Culture based component and mass percent are: bacteriological peptone 1%, YE 0.5%, sodium chloride 0.5%, pH value 6.8-7.2.
The Chinese herbal medicine Chinese gall that said method uses is dried in an oven, bake out temperature 75-80 DEG C, drying time 1-2 days; 300 orders are crushed to for subsequent use with pulverizer.
A fermentation of bacillus subtilis liquid for Chinese herbal medicine is prepared from by above-mentioned method.
The fermentation of bacillus subtilis liquid of described Chinese herbal medicine for suppressing pasteurella multocida and staphylococcus aureus, or for the preparation of suppressing the preparation of pasteurella multocida and staphylococcus aureus.Specifically for the preparation of the preparation of breeding house sterilization or the additive of animal feed.
Described bacillus subtilis Chinese herbal medicine fermented liquid is prepared into solution, and (bacillus subtilis bacterial content is 5 × 10
8cFU/ml), spray in breeding house after 1000 times of dilutions, after spraying, the concentration of breeding house bacillus subtilis AP139 is 5 × 10
5cFU/m
3, after spraying, the pasteurella multocida in breeding house, the content of staphylococcus aureus significantly reduce.
Beneficial effect of the present invention is:
The gallic fermentation of bacillus subtilis liquid of Chinese herbal medicine of the present invention, by suppressing air pasteurella multocida and staphylococcus aureus growth breeding, reduces pasteurella multocida and the staphylococcus aureus incidence of disease and the extent of injury.
The screening of bacillus subtilis of the present invention (Bacillus subtilis) AP139
Be separated from animal intestinal and obtain bacillus more than 200, respectively Escherichia coli are carried out to this bacillus more than 200, staphylococcus aureus, salmonella paratyphi, Pasteurella 439 (low toxicity Pasteurella), Pasteurella 2010 (poisoning Pasteurella), Pasteurella 2011 (strong malicious Pasteurella) bacteriostatic test, result bacillus 139 bacterial strain is best to the inhibition of Pasteurella, reach 18-25mm, bacillus 139 bacterial strain is bacillus subtilis through biochemical and molecular biology identification, and by its called after bacillus subtilis AP139.
Table 1, part Bacillus strain are to the inhibition of Pasteurella and other pathogenic bacteria
The single bacterium feature that bacillus subtilis of the present invention (Bacillus subtilis) AP139 is formed on LB solid medium is: color in white, circular, have secretion.
The 16S ribosomal rna gene sequence of bacillus subtilis (Bacillus subtilis) AP139, compared by PUBMED database, (database network address: http://blast.ncbi.nlm.nih.gov/Blast.cgi? PROGRAM=blastn & PAGE_TYPE=Bla stSearch & LINK_LOC=blasthome) find with Bacillus subtilis strain SY116S ribosomal RNA gene (JX489618.1) homology the highest.
Described bacillus subtilis (Bacillus subtilis) AP139 has significant antibacterial activity, significantly can suppress the breeding of Pasteurella in breeding house, Escherichia coli, staphylococcus aureus and salmonella.After being sprayed by the zymotic fluid of described bacterial strain, the content of the Pasteurella in breeding house, Escherichia coli, staphylococcus aureus and salmonella significantly reduces.
Bacillus subtilis of the present invention (Bacillus subtilis) AP139 is preserved in China typical culture collection center (China Center for Type Culture Collection on February 28th, 2014, be called for short CCTCC), deposit number is CCTCC NO:M 2014053, address: China. Wuhan. and Wuhan University.
Detailed description of the invention
Following examples for illustration of the present invention, but are not used in and limit the scope of the invention.
Embodiment 1:
The preparation of the gallic fermentation of bacillus subtilis liquid of Chinese herbal medicine
A, resurrection: bacillus subtilis AP139 (preserving number: CCTCC NO:M 2014053), on LB solid medium, carries out line and cultivates, cultivation temperature 30-37 DEG C, incubation time 18-24h in constant temperature biochemical cultivation case; LB solid culture based component and mass percent are: bacteriological peptone 1%, YE 0.5%, sodium chloride 0.5%, agar powder 1.5%, pH value 6.8-7.2.
B, the single clone strain of bacillus subtilis AP139 then brought back to life from picking LB solid medium are inoculated in the 500ml shaking flask that 100ml culture medium is housed and cultivate, and fermentation temperature is 35 DEG C, and rotating speed is 200r/min, and incubation time is 24h.Shaking culture based component and mass percent are: bacteriological peptone 1%, YE 0.5%, sodium chloride 0.5%, pH value 6.8-7.2.
Carry out fermentation tank pilot plant test after C, shake flask fermentation terminate: get 100ml shake flask fermentation seed liquor and be inoculated in the 10L fermentation tank of liquid amount 5L, fermentation temperature is 35 DEG C, pH value nature, mixing speed is 300r/min, fermentation 36h.Fermentation tank pilot scale medium component and mass percent are: bacteriological peptone 1%, sodium chloride 0.5%, glucose 1%, epsom salt 1%, Chinese Gall 3%, pH value nature.
Embodiment 2
Not containing the inhibition of the bacillus subtilis AP139 of Chinese gall composition fermentation
Get LB nutrient agar panel 2, get the staphylococcus aureus bacterium liquid (1 × 10 that 10 μ L activate respectively
8cFU/ml) and pig produce malicious Pasteurella bacterium liquid (1 × 10
8cFU/ml), be uniformly coated on LB solid plate, be placed in insulating box 35-37 DEG C and cultivate after 10 minutes, utilize card punch to punch on culture dish, bore dia is 5 millimeters; Do not add Chinese gall, after all the other centrifugal 3-5min of fermentation of bacillus subtilis liquid 12000r/m prepared according to the method for embodiment 1, get 80-100 μ L fermented liquid supernatant and add in the hole of culture dish, cultivate 24-48h in insulating box 35-37 DEG C.Observe size and the readability of bacillus inhibition zone, judge the antibacterial ability of tested bacterium to indicator bacteria.Result shows, the antibacterial circle diameter of fermentation of bacillus subtilis liquid to pasteurella multocida, staphylococcus aureus is respectively 22mm and 13mm.
Embodiment 3:
Chinese herbal medicine Chinese gall bacteriostatic activity is verified:
A, not containing the preparation of Chinese herbal medicine Chinese gall fermentation liquid of bacillus subtilis AP139
Electronic balance takes Chinese Gall 3g, in 100ml culture medium, carry out Shaking culture, and fermentation temperature is 35 DEG C, and rotating speed is 200r/min, and fermentation time is 24h.Medium of shaking flask fermentation composition and mass percent are: bacteriological peptone 1%, sodium chloride 0.5%, glucose 1%, epsom salt 1%, pH value nature.
B, get 2 LB nutrient agar panels, get the pasteurella multocida bacterium liquid that 10 μ L activate, be uniformly coated on a LB solid plate, get the staphylococcus aureus bacterium liquid that 10 μ L activate, be uniformly coated on second LB solid plate, be placed in insulating box 37 DEG C and cultivate 10 minutes;
C, utilize card punch to punch on culture dish, bore dia is 5 millimeters;
After D, the centrifugal 3min of Chinese herbal medicine Chinese gall culture medium solution 12000r/m, get 100 μ L supernatants and add in the hole of culture dish, cultivate 24h in insulating box 37 DEG C;
D, result show, the antibacterial circle diameter of Chinese herbal medicine Chinese gall fermentation liquid to pasteurella multocida, staphylococcus aureus is respectively 15.15mm, 15.38mm.
Embodiment 4:
The present invention contains the checking of Chinese herbal medicine gallic fermentation of bacillus subtilis liquid bacteriostatic activity:
A, get 2 LB nutrient agar panels, get the pasteurella multocida bacterium liquid that 10 μ L activate, be uniformly coated on a LB solid plate, get the staphylococcus aureus bacterium liquid that 10 μ L activate, be uniformly coated on second LB solid plate, be placed in insulating box 37 DEG C and cultivate 10 minutes;
B, utilize card punch to punch on culture dish, bore dia is 5 millimeters;
After the centrifugal 3min of C, Chinese herbal medicine gallic fermentation of bacillus subtilis liquid 12000r/m, get 100 μ L supernatants and add in the hole of culture dish, cultivate 24h in insulating box 37 DEG C;
D, result show, the antibacterial circle diameter of the gallic fermentation of bacillus subtilis liquid of Chinese herbal medicine to pasteurella multocida, staphylococcus aureus is respectively 27.40mm, 22.05mm.
Embodiment 5:
The application of fermentation of bacillus subtilis liquid in prevention plant pasteurella multocida of Chinese herbal medicine of the present invention, the steps include:
1, the gallic fermentation of bacillus subtilis liquid of Chinese herbal medicine has been prepared according to embodiment 1;
2, gallic for Chinese herbal medicine fermentation of bacillus subtilis liquid being prepared into bacillus subtilis AP139 content is 5 × 10
8cFU/mL solution, spray in breeding house with after fermentation medium 1000 times dilution, after spraying, the concentration of breeding house bacillus subtilis AP139 is 5 × 10
5cFU/m
3;
3, test is single factor experiment, totally 5 groups, experimental group 1 (the gallic fermentation of bacillus subtilis liquid spraying of Chinese herbal medicine of the present invention), test group 2 (spraying isopyknic not containing the Chinese herbal medicine fermented liquid of bacillus subtilis), experimental group 3 (spraying isopyknic bacillus subtilis AP139 zymotic fluid), experimental group 4 (spraying the benzalkonium chloride that isopyknic 1 ﹕ 2000 dilutes), experimental group 5 (spraying isopyknic distilled water, i.e. blank).
The each processed group cycle of spraying is 1 time/week, and often organize the pig cultivation quantity 20 hurdle fattening pig of totally 200 about 50 kilograms, breeding house area is 400 square metres.Test first day carries out pasteurella multocida spraying and attacks poison, and pasteurella multocida spraying dosage is 1 × 10
5cFU/m
3.
The gallic fermentation of bacillus subtilis liquid of A, Chinese herbal medicine attacks the impact of the rear breeding house Growth of Pigs performance of poison and the pasteurella multocida incidence of disease to pasteurella multocida;
This result of the test shows, the gallic fermentation of bacillus subtilis liquid of Chinese herbal medicine, when carry out spraying with pasteurella multocida attack poison, pig average daily gain (p<0.05) can be significantly improved, reduce the incidence of disease (p<0.05) of pig Pasteurella, improve production performance and the economic benefit (table 2) of pig, be suitable for large application scope.
The gallic fermentation of bacillus subtilis liquid of table 2, Chinese herbal medicine is on the impact of growing-finishing pigs performance and the incidence of disease
Note: colleague's data shoulder mark does not represent significant difference (p<0.05) containing same letter person
The gallic fermentation of bacillus subtilis liquid of B, Chinese herbal medicine is on the impact of pig house pasteurella multocida, staphylococcus aureus, Escherichia coli and salmonella quantity;
In the test end of term, KHW-8 (Anderson) eight grades of air biological sampling devices are utilized to gather breeding house air, then carry out DNA in air to extract, adopt quantitative fluorescent PCR to carry out quantitatively Pasteurella, staphylococcus aureus, Escherichia coli and salmonella.Result shows, the gallic fermentation of bacillus subtilis liquid of Chinese herbal medicine significantly reduces the content of pasteurella multocida in breeding house, staphylococcus aureus, Escherichia coli and salmonella, effectively improves micro-ecological environment in breeding house.
The gallic fermentation of bacillus subtilis liquid of table 3, Chinese herbal medicine is on pasteurella multocida, staphylococcus aureus, Escherichia coli and the impact of salmonella content in breeding house
Note: with total bacterium for internal reference, data value is (with 2
-△ △ CTrepresent)=(C
t, Target-C
t, total bacterium)
test group-(C
t, Target-C
t, always carefully bacterium)
control group, 2 of blank group
-△ △ CTequal 1
In sum, the gallic fermentation of bacillus subtilis liquid of Chinese herbal medicine of the present invention effectively can suppress the growth and breeding of pasteurella multocida and staphylococcus aureus, promote growth of animal, strengthen animal immune function, be particularly suitable for the application carrying out spraying disinfection in plant.
Claims (10)
1. a preparation method for the fermentation of bacillus subtilis liquid of Chinese herbal medicine, is characterized in that: be that bacillus subtilis (Bacillus subtilis) AP139 of CCTCC NO:M 2014053 and Chinese herbal medicine Chinese gall carry out liquid fermentation and culture and make by deposit number.
2. the preparation method of the fermentation of bacillus subtilis liquid of Chinese herbal medicine according to claim 1, is characterized in that: after fermented and cultured, bacillus subtilis bacterial content is 10
8-10
9cFU/ml.
3. the preparation method of the fermentation of bacillus subtilis liquid of Chinese herbal medicine according to claim 1, is characterized in that: it is the 1-5% of liquid fermentation medium that Chinese gall adds mass ratio.
4. the preparation method of the fermentation of bacillus subtilis liquid of Chinese herbal medicine according to claim 1, it is characterized in that: by the bacillus subtilis AP139 liquid medium obtained through Shaking culture, according to liquid fermentation medium percent by volume 1-5% inoculum concentration, be inoculated in fermentation tank, liquid fermentation medium composition and mass percent are: bacteriological peptone 1%, sodium chloride 0.5%, glucose 1%, epsom salt 1%, gallnut extract 1-5%, pH value nature; Fermentation condition is: liquid amount percent by volume 40-70%, fermentation temperature 30-40 DEG C, pH value nature, fermentation time 24-36h, rotating speed, 200-400r/min.
5. the preparation method of the fermentation of bacillus subtilis liquid of Chinese herbal medicine according to claim 4, is characterized in that: first brought back to life by the bacillus subtilis AP139 of preservation before fermentation:
Bacillus subtilis AP139, on LB solid medium, carries out line and cultivates, cultivation temperature 30-37 DEG C, incubation time 18-24h in constant temperature biochemical cultivation case; LB solid culture based component and mass percent are: bacteriological peptone 1%, YE 0.5%, sodium chloride 0.5%, agar powder 1.5%, pH value 6.8-7.2.
6. the preparation method of the fermentation of bacillus subtilis liquid of Chinese herbal medicine according to claim 5, it is characterized in that: from LB solid medium picking bring back to life the single clone strain of bacillus subtilis AP139 in 500 milliliters of conical flasks that 100 milliliters of LB fluid nutrient mediums are housed, cultivate in constant temperature biochemical rocking machine, obtain bacillus subtilis AP139 liquid medium, cultivation temperature 30-37 DEG C, rotating speed 180-220r/min, incubation time 18-24h, LB Liquid Culture based component and mass percent are: bacteriological peptone 1%, YE 0.5%, sodium chloride 0.5%, pH value 6.8-7.2.
7. the preparation method of the fermentation of bacillus subtilis liquid of Chinese herbal medicine according to claim 1, is characterized in that: Chinese herbal medicine is dried by Chinese gall in an oven, bake out temperature 75-80 DEG C, drying time 1-2 days; 300 orders are crushed to for subsequent use with pulverizer.
8. a fermentation of bacillus subtilis liquid for Chinese herbal medicine, is characterized in that, is to be prepared from by the method described in any one of claim 1-7.
9. the application process of the fermentation of bacillus subtilis liquid of Chinese herbal medicine according to claim 8, it is characterized in that, the fermentation of bacillus subtilis liquid of described Chinese herbal medicine for suppressing pasteurella multocida and staphylococcus aureus, or for the preparation of suppressing the preparation of pasteurella multocida and staphylococcus aureus.
10. the application process of the fermentation of bacillus subtilis liquid of Chinese herbal medicine according to claim 9, is characterized in that, the additive of the preparation that the fermentation of bacillus subtilis liquid of described Chinese herbal medicine is sterilized for the preparation of breeding house or animal feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410537587.XA CN104351472A (en) | 2014-10-13 | 2014-10-13 | Bacillus subtilis fermentation broth of Chinese herbal medicine as well as preparation and application of bacillus subtilis fermentation broth |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410537587.XA CN104351472A (en) | 2014-10-13 | 2014-10-13 | Bacillus subtilis fermentation broth of Chinese herbal medicine as well as preparation and application of bacillus subtilis fermentation broth |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104351472A true CN104351472A (en) | 2015-02-18 |
Family
ID=52518863
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410537587.XA Pending CN104351472A (en) | 2014-10-13 | 2014-10-13 | Bacillus subtilis fermentation broth of Chinese herbal medicine as well as preparation and application of bacillus subtilis fermentation broth |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104351472A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104940496A (en) * | 2015-06-10 | 2015-09-30 | 天农大（天津）生物科技有限公司 | Fermented traditional Chinese medicine composition containing cnidium monnieri, folium artemisiae argyi and radix sophorae flavescentis |
| CN105174493A (en) * | 2015-10-10 | 2015-12-23 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of fermentation type Chinese herbal medicine water quality modifying agent for aquaculture and application thereof |
| CN105519518A (en) * | 2015-04-08 | 2016-04-27 | 上海蓝盎电子科技发展有限公司 | Chinese herbal medicine bacterial fermentation product-containing eco-friendly harmless pathological tissue preservation and treatment solution |
| CN105941835A (en) * | 2016-06-30 | 2016-09-21 | 河南省岳氏精忠科技有限公司 | Method for preparing bacillus subtilis traditional Chinese medicine feed additive |
| CN108703253A (en) * | 2018-04-09 | 2018-10-26 | 华中农业大学 | A kind of feeding natural plants fermentation preparation and its preparation method and application preventing livestock and poultry |
| CN114097925A (en) * | 2021-09-26 | 2022-03-01 | 中国水产科学研究院淡水渔业研究中心 | Chinese gall fermentation method for promoting growth and resisting diseases of aquatic animals and application |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1831107A (en) * | 2006-03-15 | 2006-09-13 | 南京仕必得生物技术有限公司 | Traditional Chinese medicine solid type mixed culture fermentation tech. and its application |
| CN101401921A (en) * | 2008-10-30 | 2009-04-08 | 河南省龙腾高科实业有限公司 | Microbial ecological traditional Chinese medicine preparation for livestock and poultry from fermentation production of multiple bacterials and fermentation method thereof |
| CN102352330A (en) * | 2011-10-20 | 2012-02-15 | 中国科学院亚热带农业生态研究所 | Bacillus subtilis PA105 and preparation method and application thereof |
| CN103274825A (en) * | 2013-06-09 | 2013-09-04 | 苏州仁成生物科技有限公司 | Special polyelement microorganism fertilizer for preventing celery soft rot and preparation method of special polyelement microorganism fertilizer |
| CN103992968A (en) * | 2014-04-22 | 2014-08-20 | 安徽清明环保科技有限公司 | Household garbage deodorizing and sterilizing composite inoculant and preparation method thereof |
-
2014
- 2014-10-13 CN CN201410537587.XA patent/CN104351472A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1831107A (en) * | 2006-03-15 | 2006-09-13 | 南京仕必得生物技术有限公司 | Traditional Chinese medicine solid type mixed culture fermentation tech. and its application |
| CN101401921A (en) * | 2008-10-30 | 2009-04-08 | 河南省龙腾高科实业有限公司 | Microbial ecological traditional Chinese medicine preparation for livestock and poultry from fermentation production of multiple bacterials and fermentation method thereof |
| CN102352330A (en) * | 2011-10-20 | 2012-02-15 | 中国科学院亚热带农业生态研究所 | Bacillus subtilis PA105 and preparation method and application thereof |
| CN103274825A (en) * | 2013-06-09 | 2013-09-04 | 苏州仁成生物科技有限公司 | Special polyelement microorganism fertilizer for preventing celery soft rot and preparation method of special polyelement microorganism fertilizer |
| CN103992968A (en) * | 2014-04-22 | 2014-08-20 | 安徽清明环保科技有限公司 | Household garbage deodorizing and sterilizing composite inoculant and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 胡雪萍等: "芽孢杆菌抗菌作用研究", 《江西农业大学学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519518A (en) * | 2015-04-08 | 2016-04-27 | 上海蓝盎电子科技发展有限公司 | Chinese herbal medicine bacterial fermentation product-containing eco-friendly harmless pathological tissue preservation and treatment solution |
| CN104940496A (en) * | 2015-06-10 | 2015-09-30 | 天农大（天津）生物科技有限公司 | Fermented traditional Chinese medicine composition containing cnidium monnieri, folium artemisiae argyi and radix sophorae flavescentis |
| CN105174493A (en) * | 2015-10-10 | 2015-12-23 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of fermentation type Chinese herbal medicine water quality modifying agent for aquaculture and application thereof |
| CN105941835A (en) * | 2016-06-30 | 2016-09-21 | 河南省岳氏精忠科技有限公司 | Method for preparing bacillus subtilis traditional Chinese medicine feed additive |
| CN108703253A (en) * | 2018-04-09 | 2018-10-26 | 华中农业大学 | A kind of feeding natural plants fermentation preparation and its preparation method and application preventing livestock and poultry |
| CN114097925A (en) * | 2021-09-26 | 2022-03-01 | 中国水产科学研究院淡水渔业研究中心 | Chinese gall fermentation method for promoting growth and resisting diseases of aquatic animals and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104351472A (en) | Bacillus subtilis fermentation broth of Chinese herbal medicine as well as preparation and application of bacillus subtilis fermentation broth | |
| CN104099276B (en) | One bacillus subtilis AP139 and fermenting agent thereof and their application process | |
| CN109439577B (en) | Broad-spectrum antibacterial bacillus amyloliquefaciens and application thereof | |
| CN103875893B (en) | Many bacterial classifications composite microbial feed additive and preparation method thereof | |
| CN102304483A (en) | Enterococcus faecium for feeding and applications thereof | |
| CN109548962B (en) | Compound microbial agent containing lactobacillus plantarum and application thereof | |
| CN103525718B (en) | Bacillus cereus and probiotics powder thereof as well as preparation and application of probiotics powder | |
| CN105925513A (en) | Composite microbial inoculant for degrading aflatoxin B1 and preparation method thereof | |
| CN104031960A (en) | Extracting method and application of extracellular antimicrobial lipopeptides of bacillus amyloliquefaciens | |
| CN103320365A (en) | Fish-sourced aeromonas hydrophila disease antagonistic strain and application thereof | |
| CN107988117A (en) | A kind of compound probiotic decomposing agent and preparation method and application | |
| CN105420172B (en) | Microbial inoculum and the application of a kind of metribuzin pesticide residual degrading bacteria and its production | |
| CN103289914A (en) | Preparation method and application of Bacillus subtilis A16 | |
| CN108641983B (en) | Multi-effect powdery compound EM microbial inoculum and activation culture and application thereof | |
| CN100415872C (en) | DDT pesticide residue degradation bacterium and produced bacterium agent | |
| CN100368528C (en) | Bacterium for degrading pyrethroid pesticide residue and produced bacterium formulation | |
| CN105018395A (en) | Bacillus pumilus strain and application thereof in apple alternaria leaf spot prevention and control | |
| CN105166397B (en) | Brevibacillus brevis microecological preparation | |
| CN103621798A (en) | Feed prepared by using domestic garbage and preparation method of feed | |
| CN103966121B (en) | One Pseudomonas aeruginosa strain and the application in preparation antibacterials thereof | |
| CN102939966A (en) | Compound disinfectant, preparation method and application thereof | |
| CN102499097B (en) | Antibiotic-free breeding method using separately-united padding bacteria, sterilizing bacteria and organisms | |
| CN104740622A (en) | Pseudomonas aeruginosa, klebsiella and pasteurella triple-inactivated vaccine for mink | |
| CN102924142A (en) | Method for killing quarantine pests in wool processing leftovers through biological fermentation | |
| CN109294938A (en) | A kind of sick dead pig static fermentation high temperature degradation microbial inoculum and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150218 |