CN103320365A - Fish-sourced aeromonas hydrophila disease antagonistic strain and application thereof - Google Patents
Fish-sourced aeromonas hydrophila disease antagonistic strain and application thereof Download PDFInfo
- Publication number
- CN103320365A CN103320365A CN2013102854153A CN201310285415A CN103320365A CN 103320365 A CN103320365 A CN 103320365A CN 2013102854153 A CN2013102854153 A CN 2013102854153A CN 201310285415 A CN201310285415 A CN 201310285415A CN 103320365 A CN103320365 A CN 103320365A
- Authority
- CN
- China
- Prior art keywords
- fish
- aeromonas hydrophila
- ffrc
- strain
- bacillus amyloliquefaciens
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to a fish-sourced aeromonas hydrophila disease antagonistic strain and an application of the antagonistic strain and belongs to the field of aquaculture technology. A fish-sourced aeromonas hydrophila disease antagonistic strain is screened out, cultivated and added into a feed or a medicine as probiotics. The antagonistic strain is a novel antagonistic strain which is high in biological activity stability and high in specificity, is safe for cultured fish and environment, can be used as the probiotics strain for preventing and treating aeromonas hydrophila caused explosive hemorrhagic disease in large freshwater fish farming, and can be used as the main strain to prepare a microbial preparation.
Description
Technical field
The present invention relates to the Antergic Germ against Aeromonas hydrophila strain of a kind of source of fish and application thereof, be specifically related to a kind of activity resistent ingredient stability high, can be used as Antagonistic Fungi and the application thereof of Aeromonas hydrophila, belong to technical field of aquaculture.
Background technology
Aeromonas hydrophila (
Aeromonas hydrophila, A.hy) be subordinate to Aeromonas section (
Aermonadaceae), Aeromonas (
Aeromonas), this bacterium is present in water, soil and the hydrocoles body.Aeromonas hydrophila causes fish acute hemorrhagic septicemia.This disease, also claim Outbreak disease, hemorrhagic disease, hemorrhagic ascites disease, Ascites Disease etc., can infect the main breed variety of fresh water, such as cultured freshwater fishes such as grass carp, silver carp, flathead, carp, crucian carp, mandarin fish, black carp, tilapia, common eel, white crucian carp, yellow tail silver xenocypris, bream, goldfish, swamp eels, it is the popular the most a kind of hydrocoles conditioned pathogen of China.This bacterium is the Grain-negative tyrothricin, and extreme single flagellum without gemma, without pod membrane, is dispersed in arrangement, all can grow at 0 ~ 41 ℃, and the optimum pH scope of growth is 5.5 ~ 9.0.In recent years in the popular fulminant transmissible disease of south China each province cultured freshwater fish, a lot of reports are septicemia due to this bacterium,, Aeromonas hydrophila is one of the main pathogenic fungi of cultured freshwater fish fulminant transmissible disease.
The method of preventing and treating of this bacterium mainly comprises: microbiotic control, herbal control, vaccine prevention and control and probiotic bacterium prevention and control.1) microbiotic control.Microbiotic is the choice drug for the treatment of bacterial infectious disease, and microbiotic has significant inhibition or killing action to pathogenic bacteria.Studies confirm that quinolones and some aminoglycoside medicaments have stronger fungistatic effect to Aeromonas hydrophila.But be widely used along with antibiotic, different areas have produced a large amount of Resistant strain because types of medicines is different with usage in recent years, and the antibacterials sensitivity differences is very large.2) herbal control.Herbal medicine is without resistance, noresidue, and non-environmental-pollution can not cause drug-induced disease and restraining and sterilizing bacteria successful, more and more causes people's concern.Research shows that generally folk prescription uses the herbal medicine such as Turkey-galls, rheum officinale, garden burnet, peppery few and dark plum, myrobalan, pomegranate rind, cloves, garlic, the bark of ash, borneol, catechu, Root of Sixpetal Clematis to have the very strong sterilizing ability that presses down.In actual production, the herbal medicine that will have bacteriostatic action is combined into compound, suitably utilizes different herbal medicine mutually to act synergistically to prevent and treat the disease that Aeromonas hydrophila causes, can bring into play better like this bacteriostatic action of herbal medicine.There is impact in different Chinese herbal medicine extracting working methods to the bacteriostatic action of pathogenic bacteria.3) vaccine prevention and control.The vaccine that uses in the aquaculture mainly is divided into 2 classes: a class is attenuated vaccine, and another kind of is dead or the vaccine of deactivation.Attenuated vaccine contains the cause of disease alive of attenuation, energy inducing specific antibody, and similar to natural infection.The vaccine of deactivation is comprised of the toxin of some compositions of dead cause of disease or cause of disease such as bacteria lipopolysaccharide or deactivation, immunity system that can stimulating animal.This disease occurs although can use at present Evaluation of Aeromon As Hydrophila Vaccine prevention fish, because of the numerous immune response levels of this bacterium serotype lower etc. former thereby cause immune protective effect poor or unstable.4) probiotic bacterium prevention and control.Along with the appearance of resistance problem and the deterioration of environment make antibiotic use be subject to increasing test.Compare the advantage that probiotic bacterium (probiotic) relies on its non-toxic efficient with medicine control, by improving the water body environment of cultivation system, at host internal secretion nutritive substance and digestive ferment, improve host's disease resistance, improve its immunity system, reduce the sickness rate of disease, and then improve aquaculture output and economic benefit, just receiving increasing concern.At present, use more probiotic bacterium to be milk-acid bacteria, genus bacillus, yeast and the composite reactive probiotic bacterium that formed by multiple beneficial bacterial classifications such as photosynthetic bacterium, milk-acid bacterias in aquatic products disease control.
Summary of the invention
The objective of the invention is to overcome above-mentioned weak point, a kind of novel Antagonistic Fungi is provided, its biological activity stability, specificity is high, to cultured fishes and environmental safety, can be used as the probiotic strain that control Aeromonas hydrophila in large Fish-water Fish Farming causes the fulminant hemorrhagic disease.
According to technical scheme provided by the invention, the source of fish provided by the invention Antergic Germ against Aeromonas hydrophila strain is bacillus amyloliquefaciens (FFRC-S24), separates from fish intestines, and this bacterium antagonistic ability is strong, and is because separating from fish body enteron aisle, safe.The effectively infection of antagonism Aeromonas hydrophila of microbial preparation take bacillus amyloliquefaciens (FFRC-S24) as the effective active composition.
Genus bacillus is generally has a liking for the aerobic and amphimicrobian of temperature, can produce the resistance statospore tolerating various bad envrionment conditionss, and storing relatively in the fermentative production of product and transportation, other bacterium has stronger advantage.Genus bacillus has obtained large-scale promotion application as the probiotic products on the aquatic products in China, its production technique and use flow process are very ripe, and bacillus amyloliquefaciens can improve the transformation efficiency of phosphorus as probiotic additive in animal-feed industry, therefore, the bacillus amyloliquefaciens FFRC-S24 of described antagonism Aeromonas hydrophila will have wide market outlook.
One strain source of fish Antergic Germ against Aeromonas hydrophila strain is preserved in Chinese Typical Representative culture collection center, and deposit number is CCTCC NO:M2013191, and preservation date is on May 9th, 2013, and Classification And Nomenclature is bacillus amyloliquefaciens FFRC-S24
(Bacillus amyloliquefaciens)FFRC-S24.
Get bacillus amyloliquefaciens FFRC-S24 and be inoculated in the LB liquid nutrient medium, inoculum size is 20 μ L/L-10mL/L, and in 25-30 ℃ of cultivation 18-48h, makes the concentration of bacterium reach 1 * 10
9– 3 * 10
10CFU/mL; The component of LB liquid nutrient medium is: yeast extract 4-6g/L, peptone 8-15g/L, sodium-chlor 8-12g/L.
The bacillus amyloliquefaciens FFRC-S24 that is inoculated in the LB liquid nutrient medium is stirred in fish feed, so that the probiotic bacterium concentration that contains in the feed is 1 * 10
10– 5 * 10
12CFU/KG.
To be inoculated in the bacillus amyloliquefaciens FFRC-S24 of LB liquid nutrient medium, bacterial concentration is 1 * 10
9-1 * 10
11CFU/mL directly splashes to large freshwater fish culturing pool mouth, and the usage quantity of splashing is 0. 05-0.5L/m
3
Beneficial effect of the present invention: the present invention's screening is stronger to the antagonistic ability of Aeromonas hydrophila from the Antergic Germ against Aeromonas hydrophila strain of the source of fish of bacillus amyloliquefaciens FFRC-S24, to aquatic animal safety, microbial preparation as preventing and treating the large freshwater fish hemorrhagic disease that is caused by Aeromonas hydrophila has very wide application prospect.
Biological material specimens preservation: a strain source of fish Antergic Germ against Aeromonas hydrophila strain, be preserved in Chinese Typical Representative culture collection center, the address Wuhan, China, Wuhan University, deposit number is CCTCC NO:M2013191, preservation date is on May 9th, 2013, and Classification And Nomenclature is bacillus amyloliquefaciens FFRC-S24
(Bacillus amyloliquefaciens FFRC-S24)
Description of drawings
Add (experimental group) in Fig. 1 embodiment 3 feeds and do not add bacillus amyloliquefaciens (FFRC-S24) in the quantity of different time sections enteron aisle A.hy43.
Embodiment
The in-vitro screening of embodiment 1 source of fish Antergic Germ against Aeromonas hydrophila strain
Utilize LB solid medium separation of bacterial in large fresh-water fishes enteron aisle, water surrounding and bed mud, the bacterium that separation is obtained is prepared into bacteria suspension, and cut-off directly is that the little scraps of paper of 6mm soak, and is used for bacteria screening after drying.
Getting indicator (Aeromonas hydrophila) 400uL evenly coats on the LB agar plate, be that the little scraps of paper of 6mm are immersed in bacillus amyloliquefaciens (FFRC-S24) the bacterium liquid with diameter, after the flat board that scribbles indicator dries naturally, get above the scraps of paper are attached to, then put into 25-30 ℃ of constant incubator face up cultivate 1h after, be inverted and cultivate 48h.The result shows that bacillus amyloliquefaciens (FFRC-S24) reaches 7-13mm to the antibacterial circle diameter of Aeromonas hydrophila.
According to identical working method, the result shows that bacillus amyloliquefaciens (FFRC-S24) all has antagonistic ability to the Aeromonas hydrophila strain (morbidity fish body, healthy fish body, water body, bed mud) that comes from different sources.Bacillus amyloliquefaciens (FFRC-S24) is to other common in aquaculture pathogenic bacterias: Aeromonas veronii, Vibrio parahaemolyticus, Channel-catfish fish tarda, Streptococcus iniae, streptococcus agalactiae are without obvious antagonism.
Embodiment 2
Get bacillus amyloliquefaciens FFRC-S24 and be inoculated in the LB liquid nutrient medium, inoculum size is 20 μ L/L, and in 25 ℃ of cultivation 48h, makes the concentration of bacterium reach 1 * 10
9CFU/mL;
The component of LB liquid nutrient medium is: yeast extract 4g/L, peptone 8g/L, sodium-chlor 8g/L.
The bacillus amyloliquefaciens FFRC-S24 that is inoculated in the LB liquid nutrient medium is stirred in fish feed, so that the probiotic bacterium concentration that contains in the feed is 1 * 10
10CFU/KG.
Embodiment 3
Get bacillus amyloliquefaciens FFRC-S24 and be inoculated in the LB liquid nutrient medium, inoculum size is 10mL/L, and in 30 ℃ of cultivation 18h, makes the concentration of bacterium reach 2 * 10
10CFU/mL;
The component of LB liquid nutrient medium is: yeast extract 6g/L, peptone 10g/L, sodium-chlor 12g/L.
The bacillus amyloliquefaciens FFRC-S24 that is inoculated in the LB liquid nutrient medium is stirred in fish feed, so that the probiotic bacterium concentration that contains in the feed is 3 * 10
12CFU/KG.
Embodiment 4
Get bacillus amyloliquefaciens FFRC-S24 and be inoculated in the LB liquid nutrient medium, inoculum size is 5mL/L, and in 28 ℃ of cultivation 30h, makes the concentration of bacterium reach 3 * 10
10CFU/mL;
The component of LB liquid nutrient medium is: yeast extract 5g/L, peptone 10g/L, sodium-chlor 10g/L.
The bacillus amyloliquefaciens FFRC-S24 that is inoculated in the LB liquid nutrient medium is stirred in fish feed, so that the probiotic bacterium concentration that contains in the feed is 5 * 10
12CFU/KG.
Embodiment 5
Get bacillus amyloliquefaciens FFRC-S24 and be inoculated in the LB liquid nutrient medium, inoculum size is 1mL/L, and in 26 ℃ of cultivation 48h, makes the concentration of bacterium reach 2.5 * 10
10CFU/mL; The component of LB liquid nutrient medium is: yeast extract 4g/L, peptone 8g/L, sodium-chlor 12g/L.
To be inoculated in the bacillus amyloliquefaciens FFRC-S24 of LB liquid nutrient medium, bacterial concentration is 1 * 10
10CFU/mL directly splashes to large freshwater fish culturing pool mouth, and the usage quantity of splashing is 0. 05L/m
3
Get bacillus amyloliquefaciens FFRC-S24 and be inoculated in the LB liquid nutrient medium, inoculum size is 3mL/L, and in 27 ℃ of cultivation 25h, makes the concentration of bacterium reach 1 * 10
10CFU/mL; The component of LB liquid nutrient medium is: yeast extract 6g/L, peptone 15g/L, sodium-chlor 8g/L.
To be inoculated in the bacillus amyloliquefaciens FFRC-S24 of LB liquid nutrient medium, bacterial concentration is 9 * 10
9CFU/mL directly splashes to large freshwater fish culturing pool mouth, and the usage quantity of splashing is 0.2L/m
3
Get bacillus amyloliquefaciens FFRC-S24 and be inoculated in the LB liquid nutrient medium, inoculum size is 8mL/L, and in 29 ℃ of cultivation 31h, makes the concentration of bacterium reach 3 * 10
10CFU/mL; The component of LB liquid nutrient medium is: yeast extract 6g/L, peptone 15g/L, sodium-chlor 12g/L.
The bacillus amyloliquefaciens FFRC-S24 of LB liquid nutrient medium, bacterial concentration 1 * 10 will be inoculated in
11CFU/mL directly splashes to large freshwater fish culturing pool mouth, and the usage quantity of splashing is 0.4L/m
3
Embodiment 3 bacillus amyloliquefaciens (FFRC-S24) are to enteron aisle cause of disease restraining effect
With A.hy43 shaking culture 18h in 30 ℃ of LB liquid nutrient mediums, it is put in the aquarium, so that the concentration of Aeromonas hydrophila reaches 10 in the aquarium
7CFU/mL soaks and gets three tail Megalobrama amblycephalas after one day and carry out enteron aisle sampling, is designated as 0h.Then with A.hy43
GFPThe Megalobrama amblycephala that soaked is divided into two groups, puts into new aquarium, and one group is experimental group, and one group is control group.
Experimental group is thrown something and fed and is mixed with the feed of probiotic bacterium S24, and the control group normal diet of throwing something and feeding is got experimental group and each 3 tail fish of control group at 12h, 24h, 36h, 48h respectively, by spirit lamp, aseptic technique gathers before, during and after the Megalobrama amblycephala each about 1cm of intestines, places 1.5 mL centrifuge tubes after the sterilization, weighs.
To gather enteron aisle and wash with sterile saline, and add respectively afterwards the 2mL sterile saline and carry out homogenate.Homogenate is diluted respectively 10 times, 100 times, 1000 times with sterile saline, get the diluted liquid of 3 gradients and organize each 100 μ L of stoste evenly to coat the LB nutrient agar plate (containing kantlex 60 μ g/mL) that contains kantlex, place 30 ° of C biochemical cultivation cases to cultivate 18 h.At fluorescence microscopy Microscopic observation bacterium colony fluorescence, and counting.
The result shows that the quantity of A.hy43 in the experimental group enteron aisle all is less than control group.Between 0-12h, the quantity of Aeromonas hydrophila is in the decline stage, and the amount of bacteria of each group all begins to increase behind the 12h.But it is slow than control group that the experimental group of the S24 that throws something and feeds gathers way.
Concrete data are as shown in table 1.
Add (experimental group) in table 1 feed and do not add bacillus amyloliquefaciens (FFRC-S24) in the quantity of different time sections enteron aisle A.hy43
The experiment of embodiment 4 protection ratios
Select 30 urosomes to weigh the healthy Megalobrama amblycephala of 75 ± 0.5g, in aquarium, support temporarily 7d, begin test.Test is divided into two groups, every group of each 15 tail.The control group normal diet of throwing something and feeding, experimental group are thrown something and fed and 2.1 are contained the bacillus amyloliquefaciens feed.Throw something and feed behind the 2w, soak with A.hy43 respectively and attack poison, the A.hy43 concentration in each aquarium is 10
7CFU/ml, breeding observing 7d, the mortality ratio of each group of statistics is calculated immune protective rate according to following formula.Immune protective rate (RPS)=[1-(immune group mortality ratio/control group mortality ratio)] * 100%.
The mortality of each group sees Table 2, and the protection ratio that we calculate S24 from table is 60%.
Table 2 protection ratio experimental result
Claims (4)
1. a strain source of fish Antergic Germ against Aeromonas hydrophila strain is preserved in Chinese Typical Representative culture collection center, and deposit number is CCTCC NO:M2013191, and preservation date is on May 9th, 2013, and Classification And Nomenclature is bacillus amyloliquefaciens FFRC-S24
(Bacillus amyloliquefaciens)FFRC-S24.
2. the application of the described source of fish of claim 1 Antergic Germ against Aeromonas hydrophila strain, it is characterized in that: get bacillus amyloliquefaciens FFRC-S24 and be inoculated in the LB liquid nutrient medium, inoculum size is 20 μ L/L-10mL/L, and in 25-30 ℃ of cultivation 18-48h, makes the concentration of bacterium reach 1 * 10
9– 3 * 10
10CFU/mL;
The component of LB liquid nutrient medium is: yeast extract 4-6g/L, peptone 8-15g/L, sodium-chlor 8-12g/L.
3. the as claimed in claim 2 application of source of fish Antergic Germ against Aeromonas hydrophila strain is characterized in that: the bacillus amyloliquefaciens FFRC-S24 that will be inoculated in the LB liquid nutrient medium stirs in fish feed, so that the probiotic bacterium concentration that contains in the feed is 1 * 10
10– 5 * 10
12CFU/KG.
4. the as claimed in claim 2 application of source of fish Antergic Germ against Aeromonas hydrophila strain is characterized in that: will be inoculated in the bacillus amyloliquefaciens FFRC-S24 of LB liquid nutrient medium, bacterial concentration is 1 * 10
9-1 * 10
11CFU/mL directly splashes to large freshwater fish culturing pool mouth, and the usage quantity of splashing is 0. 05-0.5L/m
3
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310285415.3A CN103320365B (en) | 2013-07-09 | 2013-07-09 | Fish-sourced aeromonas hydrophila disease antagonistic strain and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310285415.3A CN103320365B (en) | 2013-07-09 | 2013-07-09 | Fish-sourced aeromonas hydrophila disease antagonistic strain and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103320365A true CN103320365A (en) | 2013-09-25 |
| CN103320365B CN103320365B (en) | 2015-07-08 |
Family
ID=49189411
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310285415.3A Expired - Fee Related CN103320365B (en) | 2013-07-09 | 2013-07-09 | Fish-sourced aeromonas hydrophila disease antagonistic strain and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103320365B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103966136A (en) * | 2014-05-14 | 2014-08-06 | 北京鑫洋水产高新技术有限公司 | Double-shell aeromonas and application thereof as well as feed and application thereof |
| CN105349463A (en) * | 2015-11-27 | 2016-02-24 | 暨南大学 | Pseudomonas strain and application thereof |
| CN106318880A (en) * | 2015-06-15 | 2017-01-11 | 中国科学院微生物研究所 | Bacillus amyloliquefaciens and its bacterial depressant and use |
| CN109439565A (en) * | 2018-09-14 | 2019-03-08 | 湖南师范大学 | One plant of Pediococcus pentosaceus bacterial strain, its probiotics and preparation method thereof |
| CN110438043A (en) * | 2019-08-20 | 2019-11-12 | 中国水产科学研究院长江水产研究所 | A kind of Methylotrophic bacillus YFI-1 and preparing the application in aquatic products bacterium bacteriostatic agent |
| CN110468071A (en) * | 2019-08-20 | 2019-11-19 | 中国水产科学研究院长江水产研究所 | Composite bacteria agent containing Methylotrophic bacillus and the application in the drug for preparing aquatic animal disease |
| CN113663001A (en) * | 2021-08-24 | 2021-11-19 | 四川成康动物药业有限公司 | Essence composition containing rhubarb and gallnut and preparation method and device thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108464413A (en) * | 2018-06-28 | 2018-08-31 | 中国水产科学研究院淡水渔业研究中心 | A kind of pond industrialization circulating water culture system megalobrama amblycephala functional feed and preparation method thereof |
-
2013
- 2013-07-09 CN CN201310285415.3A patent/CN103320365B/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
|---|
| 安健等: "解淀粉芽孢杆菌的安全性分析", 《动物医学进展》 * |
| 曹海鹏等: "鲟源病原性嗜水气单胞菌拮抗芽孢杆菌的鉴定及其生物学特性", 《微生物学通报》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103966136A (en) * | 2014-05-14 | 2014-08-06 | 北京鑫洋水产高新技术有限公司 | Double-shell aeromonas and application thereof as well as feed and application thereof |
| CN103966136B (en) * | 2014-05-14 | 2016-03-16 | 北京鑫洋水产高新技术有限公司 | A kind of bivalve Aeromonas and uses thereof and feed and uses thereof |
| CN106318880A (en) * | 2015-06-15 | 2017-01-11 | 中国科学院微生物研究所 | Bacillus amyloliquefaciens and its bacterial depressant and use |
| CN106318880B (en) * | 2015-06-15 | 2019-09-20 | 中国科学院微生物研究所 | One bacillus amyloliquefaciens, bacteriostatic agent prepared therefrom and purposes |
| CN105349463A (en) * | 2015-11-27 | 2016-02-24 | 暨南大学 | Pseudomonas strain and application thereof |
| CN105349463B (en) * | 2015-11-27 | 2018-10-09 | 暨南大学 | A kind of pseudomonad strain and its application |
| CN109439565A (en) * | 2018-09-14 | 2019-03-08 | 湖南师范大学 | One plant of Pediococcus pentosaceus bacterial strain, its probiotics and preparation method thereof |
| CN110438043A (en) * | 2019-08-20 | 2019-11-12 | 中国水产科学研究院长江水产研究所 | A kind of Methylotrophic bacillus YFI-1 and preparing the application in aquatic products bacterium bacteriostatic agent |
| CN110468071A (en) * | 2019-08-20 | 2019-11-19 | 中国水产科学研究院长江水产研究所 | Composite bacteria agent containing Methylotrophic bacillus and the application in the drug for preparing aquatic animal disease |
| CN110438043B (en) * | 2019-08-20 | 2020-12-22 | 中国水产科学研究院长江水产研究所 | Bacillus methylotrophicus YFI-1 and application thereof in preparation of aquatic bacteria bacteriostatic agent |
| CN113663001A (en) * | 2021-08-24 | 2021-11-19 | 四川成康动物药业有限公司 | Essence composition containing rhubarb and gallnut and preparation method and device thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103320365B (en) | 2015-07-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103320365B (en) | Fish-sourced aeromonas hydrophila disease antagonistic strain and application thereof | |
| Zhou et al. | Role and functions of beneficial microorganisms in sustainable aquaculture | |
| Gupta et al. | Growth performance, feed utilization, digestive enzyme activity, innate immunity and protection against Vibrio harveyi of freshwater prawn, Macrobrachium rosenbergii fed diets supplemented with Bacillus coagulans | |
| CN106754551A (en) | A kind of bacterium amount lactobacillus preparation high and preparation method and application | |
| CN105368749B (en) | One bacillus subtilis and feed addictive comprising the bacterial strain | |
| CN109749966A (en) | One plant of Pseudoalteromonas and its application as probiotics on aquatic products | |
| CN103525718B (en) | Bacillus cereus and probiotics powder thereof as well as preparation and application of probiotics powder | |
| CN104195067A (en) | Bacillus amyloliquefaciens and application thereof in aquaculture | |
| Sivagnanavelmurugan et al. | Effect of Sargassum wightii fucoidan on growth and disease resistance to Vibrio parahaemolyticus in Penaeus monodon post‐larvae | |
| CN102283328A (en) | Multifunctional compound micro-ecological preparation for culturing holothurian | |
| CN102154176A (en) | Turbot pathogenic strain and inactivated vaccine for ascites disease | |
| CN102559534B (en) | Bacillus cereus, and preparation and application of bacillus cereus | |
| CN104774781B (en) | One bacillus subtilis DCU and application thereof | |
| CN103966122B (en) | One strain Providence and the application in preparation antibacterials thereof | |
| CN102994417A (en) | Bacillus pumilus and application thereof in prevention and control of comma bacillus in aquatic product | |
| CN103966135B (en) | A kind of bacillus cereus and uses thereof and feed and uses thereof | |
| Dashtiannasb et al. | The effect of ethanol extract of a macroalgae Laurencia snyderia on growth parameters and vibriosis resistance in shrimp Litopenaeus vannamei | |
| Duarte et al. | Saccharomyces cerevisiae used as probiotic: Strains characterization and cell viability | |
| Olusola et al. | The potential of different extraction methods of soursop (Annona muricata Linn) leaves as antimicrobial agents for aquatic animals | |
| CN104726359A (en) | Bacillus pumilus BP and application thereof | |
| CN102559533A (en) | Bacillus atrophaeus, and preparation and application of bacillus atrophaeus | |
| CN103966121B (en) | One Pseudomonas aeruginosa strain and the application in preparation antibacterials thereof | |
| CN104195068A (en) | Composite probiotics used for preventing and treating prawn early mortality syndrome and application thereof | |
| CN103966123B (en) | One strain Huo Shi enterobacteria and the application in preparation antibacterials thereof | |
| CN106987543B (en) | A kind of serratia marcescens BSFL-6 in stratiomyiid enteron aisle source and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150708 Termination date: 20170709 |