CN103966135B - A kind of bacillus cereus and uses thereof and feed and uses thereof - Google Patents
A kind of bacillus cereus and uses thereof and feed and uses thereof Download PDFInfo
- Publication number
- CN103966135B CN103966135B CN201410203206.4A CN201410203206A CN103966135B CN 103966135 B CN103966135 B CN 103966135B CN 201410203206 A CN201410203206 A CN 201410203206A CN 103966135 B CN103966135 B CN 103966135B
- Authority
- CN
- China
- Prior art keywords
- bacillus cereus
- feed
- prawn
- present
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000193755 Bacillus cereus Species 0.000 title claims abstract description 52
- 241000238557 Decapoda Species 0.000 claims abstract description 43
- 235000015097 nutrients Nutrition 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 9
- 241000894006 Bacteria Species 0.000 claims description 25
- 108090000623 proteins and genes Proteins 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 9
- 229940088594 vitamin Drugs 0.000 claims description 5
- 235000013343 vitamin Nutrition 0.000 claims description 5
- 239000011782 vitamin Substances 0.000 claims description 5
- 229930003231 vitamin Natural products 0.000 claims description 5
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 5
- 150000001720 carbohydrates Chemical class 0.000 claims description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 4
- 150000002632 lipids Chemical class 0.000 claims description 4
- 239000011707 mineral Substances 0.000 claims description 4
- 235000010755 mineral Nutrition 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 229940075612 bacillus cereus Drugs 0.000 claims description 2
- 238000012258 culturing Methods 0.000 abstract description 6
- 230000003053 immunization Effects 0.000 abstract description 6
- 239000004382 Amylase Substances 0.000 abstract description 5
- 102000013142 Amylases Human genes 0.000 abstract description 5
- 108010065511 Amylases Proteins 0.000 abstract description 5
- 235000019418 amylase Nutrition 0.000 abstract description 5
- 230000003248 secreting effect Effects 0.000 abstract description 3
- 238000004904 shortening Methods 0.000 abstract description 3
- 230000009471 action Effects 0.000 abstract description 2
- 230000000529 probiotic effect Effects 0.000 description 15
- 239000006041 probiotic Substances 0.000 description 14
- 235000018291 probiotics Nutrition 0.000 description 14
- 241001465754 Metazoa Species 0.000 description 13
- 238000012360 testing method Methods 0.000 description 12
- 241000238553 Litopenaeus vannamei Species 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000000034 method Methods 0.000 description 9
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 235000012054 meals Nutrition 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 102000019197 Superoxide Dismutase Human genes 0.000 description 5
- 108010012715 Superoxide dismutase Proteins 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 230000037396 body weight Effects 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000012216 screening Methods 0.000 description 5
- 239000013535 sea water Substances 0.000 description 5
- 102000013563 Acid Phosphatase Human genes 0.000 description 4
- 108010051457 Acid Phosphatase Proteins 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 235000019198 oils Nutrition 0.000 description 4
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 3
- 235000017060 Arachis glabrata Nutrition 0.000 description 3
- 244000105624 Arachis hypogaea Species 0.000 description 3
- 235000010777 Arachis hypogaea Nutrition 0.000 description 3
- 235000018262 Arachis monticola Nutrition 0.000 description 3
- 235000019733 Fish meal Nutrition 0.000 description 3
- 238000010162 Tukey test Methods 0.000 description 3
- 241000607618 Vibrio harveyi Species 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- MBLBDJOUHNCFQT-LXGUWJNJSA-N aldehydo-N-acetyl-D-glucosamine Chemical compound CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000004467 fishmeal Substances 0.000 description 3
- 210000000087 hemolymph Anatomy 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000001543 one-way ANOVA Methods 0.000 description 3
- 235000020232 peanut Nutrition 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 235000019786 weight gain Nutrition 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 description 2
- 241000238421 Arthropoda Species 0.000 description 2
- MJVAVZPDRWSRRC-UHFFFAOYSA-N Menadione Chemical compound C1=CC=C2C(=O)C(C)=CC(=O)C2=C1 MJVAVZPDRWSRRC-UHFFFAOYSA-N 0.000 description 2
- 108010014251 Muramidase Proteins 0.000 description 2
- 102000016943 Muramidase Human genes 0.000 description 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 241000238550 Penaeidae Species 0.000 description 2
- 241000927735 Penaeus Species 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 2
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 description 2
- 239000001527 calcium lactate Substances 0.000 description 2
- 229960002401 calcium lactate Drugs 0.000 description 2
- 235000011086 calcium lactate Nutrition 0.000 description 2
- 239000001506 calcium phosphate Substances 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 235000019197 fats Nutrition 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 244000005709 gut microbiome Species 0.000 description 2
- 238000002649 immunization Methods 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 235000010335 lysozyme Nutrition 0.000 description 2
- 229960000274 lysozyme Drugs 0.000 description 2
- 239000004325 lysozyme Substances 0.000 description 2
- 229910000150 monocalcium phosphate Inorganic materials 0.000 description 2
- 235000019691 monocalcium phosphate Nutrition 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 229940083466 soybean lecithin Drugs 0.000 description 2
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 1
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 239000005996 Blood meal Substances 0.000 description 1
- 241000255789 Bombyx mori Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 102000052508 Lipopolysaccharide-binding protein Human genes 0.000 description 1
- 108010053632 Lipopolysaccharide-binding protein Proteins 0.000 description 1
- 108010064696 N,O-diacetylmuramidase Proteins 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 1
- 241000108664 Nitrobacteria Species 0.000 description 1
- 241000237502 Ostreidae Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 239000013614 RNA sample Substances 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 241000238371 Sepiidae Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 229930003471 Vitamin B2 Natural products 0.000 description 1
- 229930003756 Vitamin B7 Natural products 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 229920002494 Zein Polymers 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 description 1
- 229960002079 calcium pantothenate Drugs 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000004163 cytometry Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 235000021323 fish oil Nutrition 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 125000005456 glyceride group Chemical group 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000003677 hemocyte Anatomy 0.000 description 1
- 229940000351 hemocyte Drugs 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 229960001438 immunostimulant agent Drugs 0.000 description 1
- 239000003022 immunostimulating agent Substances 0.000 description 1
- 230000003308 immunostimulating effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 235000021388 linseed oil Nutrition 0.000 description 1
- 239000000944 linseed oil Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
- 235000005152 nicotinamide Nutrition 0.000 description 1
- 239000011570 nicotinamide Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000006180 nutrition needs Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 235000020636 oyster Nutrition 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
- NEGYEDYHPHMHGK-UHFFFAOYSA-N para-methoxyamphetamine Chemical compound COC1=CC=C(CC(C)N)C=C1 NEGYEDYHPHMHGK-UHFFFAOYSA-N 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000003910 polypeptide antibiotic agent Substances 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000019254 respiratory burst Effects 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 108010027322 single cell proteins Proteins 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 235000019158 vitamin B6 Nutrition 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
- 235000011912 vitamin B7 Nutrition 0.000 description 1
- 239000011735 vitamin B7 Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 235000012711 vitamin K3 Nutrition 0.000 description 1
- 239000011652 vitamin K3 Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 239000005019 zein Substances 0.000 description 1
- 229940093612 zein Drugs 0.000 description 1
Abstract
The invention discloses a kind of bacillus cereus, does is the preserving number of this bacillus cereus CGMCC? No.9139.The invention also discloses a kind of feed, this feed contains described bacillus cereus and basal nutrient material.The invention also discloses described bacillus cereus and/or the purposes of described feed in prawn is cultivated.In addition, the invention also discloses described bacillus cereus and prepare the purposes in prawn feed.Bacillus cereus of the present invention can secreting amylase, significantly improves the growth performance of prawn, Shortening culturing period, and safety can not produce undesirable action to prawn.And, bacillus cereus of the present invention is added in prawn feed and feeds the immunizing power that prawn can strengthen prawn significantly.
Description
Technical field
The invention belongs to microorganism field, particularly, relate to a kind of bacillus cereus (Bacilluscereus) and uses thereof and the feed containing this bacillus cereus and uses thereof.
Background technology
Penaeus vannamei (PenaeusvannameiBoone), formal name used at school Litoenaeus vannamei, belonging to Arthropoda (Arthropoda), Crustachia (Crustacea), Decapoda (Decapoda), swimming suborder (Natantia), Penaeidae (Penaeidae), Penaeus (Penaeus), is wide temperature eurysalinity torrid zone shrimps.Along with the development of shrimp culture industry and the raising of intensive degree, various disease frequently occurs, the development of serious restriction shrimp culture industry.The abuse of a large amount of microbiotic and chemicals not only makes the immunity degradation of animal itself, and normal intestinal flora is destroyed, and causes the pollution of ecotope.In recent years, the ecologic breeding received much concern became the key ways breaking through sea farming undoubtedly, and wherein probiotic bacterium is developed to as the important means in ecologic breeding.At present, about the fundamental research of probiotic bacterium is weaker, systematic deep structure research is especially lacked.Therefore, to aquatic animal intestine microbial diversity with can the research of Macroflora colonization be the most important condition of probiotic bacterium development and application.
Traditional disease control means mainly use microbiotic and chemicals, not only can destroy the normal colony balance of animal body, cause animal body lower immune function, and the increase of Resistant strain in animal body can be caused, strengthen the difficulty of disease control further.Owing to lacking clear and definite usage criteria, for a long time, the abuse of microbiotic and chemicals, constitutes very large threat to ecotope and human health, and the food-safety problem that drug residue brings also emerges in an endless stream.Ensuring food safety, pursue green ecological cultivation cry more and more higher today, probiotic bacterium receives increasing concern as a kind of disease control means of highly effective and safe.Probiotic bacterium suppression pathogenic bacteria, regulating intestinal canal colony balance, help digest with enhancing body immunizing power, improvement water quality etc. in play remarkable effect.Compare with chemicals with microbiotic, probiotic bacterium has drawn from animal body or its breeding environment, has highly effective and safe, the natural advantage such as pollution-free.
The probiotic bacterium of applying in current aquaculture at home mainly contains photosynthetic bacterium, Antagonistic Fungi, and nutrition and product digestive ferment micropopulation (milk-acid bacteria, yeast etc.), improve water quality flora (nitrobacteria, denitrifying bacteria etc.).These bacteriums are mostly derived from terrestrial animal or use for reference the development of terrestrial animal thinking, and be applied in aquatic products the deficiency existing and be difficult to survive and be difficult in animal body field planting in breeding environment, its effect is difficult to play.The screening principle of probiotic bacterium is widely accepted in the world: must be harmless to host; Can field planting breeding in host; The position needing it to play function can be arrived; Actual effect in vitro and in vivo wants consistent; Not containing Disease-causing gene and drug resistant gene.Endogenous and the external source of prebiotic bacterial classification is the important factor affecting probiotic effect, screen probiotic bacterium in organism or the natural surroundings of its existence is also be preferably the most effective approach simultaneously, and Gate thinks that in healthy animal, dominant bacteria or secondary dominant bacteria can as the sources of probiotic bacterium normally.Dominant microflora has can field planting to host and environmentally friendly advantage, has that be developed as efficiently can the potentiality of field planting probiotic bacterium.And for a long time, the screening study of aquatic animal specialty probiotic bacterium focuses mostly in intestinal microflora qualification and the screening of some known common probiotic bacteriums (milk-acid bacteria, genus bacillus etc.), and the probiotic bacterium that screening has an excellent properties to be added in prawn feed thus promotes that the healthy growth of prawn is significant.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of and can promote bacillus cereus of prawn healthy growth and uses thereof and the feed containing this bacillus cereus and uses thereof.
To achieve these goals, the present inventor has carried out a large amount of screening experiments, and result screens a strain performance preferably bacillus cereus, therefore, first aspect, the invention provides a kind of bacillus cereus, and the preserving number of this bacillus cereus is CGMCCNo.9139.
Second aspect, the invention provides a kind of feed, and this feed contains bacillus cereus described in first aspect and basal nutrient material.
The third aspect, the invention provides the bacillus cereus described in first aspect and/or the purposes of the feed described in second aspect in prawn is cultivated.
Fourth aspect, the bacillus cereus that the invention provides described in first aspect is preparing the purposes in prawn feed.
Bacillus cereus of the present invention can secreting amylase, can improve the abilities of digestive and absorption of prawn, significantly improve the growth performance of prawn, Shortening culturing period, and safety can not produce undesirable action to prawn.And, bacillus cereus of the present invention is added in prawn feed and feeds the immunizing power that prawn can strengthen prawn significantly.
Other features and advantages of the present invention are described in detail in embodiment part subsequently.
Biological deposits
Bacillus cereus of the present invention is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on May 9th, 2014 and (is abbreviated as CGMCC, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101), deposit number is CGMCCNo.9139.
Embodiment
Below the specific embodiment of the present invention is described in detail.Should be understood that, embodiment described herein, only for instruction and explanation of the present invention, is not limited to the present invention.
The preserving number of bacillus cereus provided by the invention is CGMCCNo.9139.Cultivated on 2216E substratum by bacillus cereus of the present invention, its cultural characteristic is as shown in table 1 below.
Table 1
| Color | Shape | Highly | Edge | Transparency | Slickness | Diameter (mm) | Gramstaining |
| Oyster white | Circle | Convex | Quan Yuan | Opaque | Smooth moistening | 6 | + |
Feed provided by the invention contains above-mentioned bacillus cereus and basal nutrient material.
According to the present invention, to the not special requirement of the content in feed of bacillus cereus, under preferable case, in the viable bacteria body of bacillus cereus, the content of described bacillus cereus in feed is 10
6-10
8cfu/g." cfu " is colony-forming unit, can be recorded by the method for plate culture count.
According to the present invention, described basal nutrient material can be the conventional nutritive substance for aquaculture (particularly prawn culturing), comprises protein, lipid, carbohydrate, VITAMIN and mineral substance etc.The content of basal nutrient material can carry out appropriate selection according to the nutritional needs of prawn, under preferable case, with the gross weight of described feed for benchmark, the content of protein is 20-55 % by weight, the content of lipid is 15-20 % by weight, the content of carbohydrate is 20-35 % by weight, the content of VITAMIN is 1-1.5 % by weight and the content of mineral substance is 0.2-3 % by weight.Basal nutrient material can providing for the raw material preparing aquatic animal feed by routine, such as, described basal nutrient material can by animal feedstuff raw material (as fish meal, liver powder, shrimp med, shrimp shell meal, crab meat, crab shell powder, chicken powder, blood meal, silkworm chrysalis etc.), vegetality feedstuff is (as dregs of beans, peanut meal, flour, cotton dregs, the dish dregs of rice, single cell protein (yeast), the vinasse dregs of rice, Zein powder etc.), glyceride stock is (as fish oil, phosphatide oil, soya-bean oil, cuttlefish cream, Semen Maydis oil, plam oil, linseed oil wet goods), VITAMIN is (as V
cand V
edeng) etc. coordinate provide, such as, thered is provided by the mixture of " fish meal, soyflour, peanut meal, yeast, soybean lecithin, shrimp shell meal, wheat-flour, seed fat, calcium lactate and monocalcium phosphate ", adopting which kind of proportioning to carry out being mixed to get described mixture is that those skilled in the art can be easy to determine, does not repeat them here.
Usually, described feed contains a certain amount of water, and such as, in feed, moisture content is generally 8-12% weight.In addition, in order to improve the quality of prawn further and promote to cultivate, additive can also be contained in described feed, as phagostimulant, immunostimulant, growth stimulant, high temperature resistant zymin, antioxidant etc.The content of additive is generally 5-10 % by weight.
Present invention also offers above-mentioned bacillus cereus and/or the purposes of feed in prawn is cultivated.
In addition, present invention also offers above-mentioned bacillus cereus and prepare the purposes in prawn feed.
Below will be described the present invention by embodiment.
Embodiment 1
The present embodiment is used for illustrating the enzymatic productivity of bacillus cereus of the present invention.
By bacillus cereus dibbling of the present invention on amylase Selective agar medium, cultivate 48h for 28 DEG C.If periphery of bacterial colonies produces transparent ring, illustrate that bacterial strain has corresponding product enzyme activity, measure and record the transparent ring of periphery of bacterial colonies and colony diameter ratio.Relevant culture medium prescription is as follows:
Amylase Selective agar medium: Zulkovsky starch 10g, peptone 5g, yeast extract paste 1g, agar 16g, artificial seawater 1000mL; Adjust pH to 7.4,121.5 DEG C of sterilizing 20min are for subsequent use.
Recording and producing enzymic hydrolysis circle is 3.00 with colony diameter ratio (H/D), bacillus cereus energy secreting amylase of the present invention is described, thus there is the ability of certain promoting digestion absorption, the utilization ratio of feed in prawn culturing process can be improved, promote the healthy growth of prawn.
Embodiment 2
The present embodiment is used for illustrating the security of bacillus cereus of the present invention.
(1) hemolytic test
By dull and stereotyped for aseptic Sanguis Naemorhedi rewarming to 25 DEG C, then the bacillus cereus of activation is inoculated on Sanguis Naemorhedi flat board, after cultivating 48h in 25 DEG C of constant incubators, observes and find that periphery of bacterial colonies does not occur zone of hemolysis, show that this bacterium is without haematolysis ability.
(2) feed is fed
The nutritive ingredient of feed is: the fish meal of 38 % by weight, the soyflour of 19 % by weight, 6 % by weight peanut meal, 4 % by weight yeast, 5 % by weight soybean lecithin, 10 % by weight shrimp shell meal, 10.5 % by weight wheat-flour, 1.5 % by weight seed fat, 0.4 % by weight calcium lactate, 2.6 % by weight monocalcium phosphate, 3 % by weight pre-mixing component (concrete composition is: every kilogram of feed contains inositol: 3000.0mg; Niacinamide: 2000.0mg; Vitamin H: 20.0mg; Choline: 4%; Calcium pantothenate: 1200.0mg; Folic acid: 150.0mg; Vitamin A: 300000IU; Vitamins D: 150000IU; Vitamin-E: 5000.0mg; Vitamin K3: 150.0mg; Vitamins B: 200.0mg; Lin Suanna Vitamin B2 Sodium Phosphate: 200.0mg; Vitamin B6: 200.0mg; Vitamins C: 6000.0mg; Cu:220mg; Fe:8000mg; Zn:400; Mn:1200mg; Mg:12000mg; Se:20mg; I:25mg; Co:5000mg).
Select the young shrimp of healthy Penaeus vannamei (purchased from South Sinkiang, Hainan slab bridge cultivation factory) to carry out bacterium safety experiment, test and support three days temporarily with shrimp.Select in the same size, volume is about the bubble chamber 6 of 50L, and antiseptic sea water 32L contained by each bucket, and accurate counting Penaeus vannamei 100 tail puts into bubble chamber, supports temporarily and within one day, is divided into 2 groups at random afterwards, 1 treatment group and 1 control group, often organize three repetitions.Treatment group adds containing bacillus cereus of the present invention that (concentration is 10
7cfu/g) feed.Manually throw something and feed to fed state, throw something and feed every day 4 times, Feeding time: morning 6:00, noon 10:00, at dusk 17:00, evening 22:00.The feed that control group uses is not containing bacillus cereus of the present invention, and other step is consistent, Therapy lasted seven days, experimental session continuous charge.The prawn of test group and control group does not all occur mortality and without significant difference, Dissection test group does not have pathological phenomenon yet.
The result of the present embodiment illustrates that bacillus cereus of the present invention does not have toxicity to prawn, can use safely.
Embodiment 3
The present embodiment is used for the impact of bacillus cereus of the present invention on prawn immunity function is described.
It is 3m × 3m that the Penaeus vannamei (purchased from South Sinkiang, Hainan slab bridge cultivation factory) of unified specification is grown on the end, water level height is in the culturing pool of 0.7m, seawater directly extracts from ocean, through sand filter after adjust water temperature to 15-18 DEG C after for cultivate seawater used, continue aeration.Test Penaeus vannamei is supported temporarily and is divided into groups after 3 days, and test is divided into 2 groups, treatment group and control group.Treatment group is thrown something and fed containing the feed (other compositions are in the same manner as in Example 2) of bacillus cereus of the present invention, and control group fed does not add the feed of bacillus cereus of the present invention.In breeding process, water temperature remains on 16.8-18 DEG C, salinity 20-23, pH7.5-7.9, and dissolved oxygen is not less than 7.5mg/L; Manually throw something and feed to fed state, throw something and feed every day 4 times, Feeding time: morning 6:00, noon 10:00, at dusk 17:00, evening 22:00.Within every two days, change water once to maintain water quality, feeding period is 28 days.Respectively at early 7 points at per weekend, namely the 7th, 14,21,28 day.A random selecting 5-10 Penaeus vannamei from each repetition that immune indexes determination experiment is respectively organized, gets blood with disposable sterilized injector, gets blood 1.5mL at every turn from Penaeus vannamei abdomen blood sinus.Hemolymph is placed in after ice bath mixes, from hemolymph mixed solution, draws the Eppendorf pipe that 1mL is placed in 1.5mL detect for cytometry, phagocytic activity and respiratory burst immediately.At remaining hemolymph low temperature ultracentrifuge 4 DEG C, the centrifugal 10min of 3000g obtains serum.Be stored in-20 DEG C for detecting other immune indexes; Get the Penaeus vannamei after blood and aseptically dissect acquisition enteron aisle for analysis of intestinal microflora.All indexs have been surveyed in 1 day after getting blood.Measuring method and the result of each index are as follows:
(1) serum lysozyme activities reached: the mensuration of serum lysozyme activities reached.The N,O-Diacetylmuramidase detection kit that this index measurement adopts Nanjing to build up Bioengineering Research Institute's production is carried out, and operating process is carried out according to the operation instruction of test kit.
(2) acid phosphatase: the mensuration that acid phosphatase (ACP) is active.The acid phosphatase enzyme detection kit that this index measurement adopts Nanjing to build up Bioengineering Research Institute's production carries out, and operating process is carried out according to the operation instruction of test kit.
(3) serum superoxide dismutases (SOD): the mensuration that serum superoxide dismutases (SOD) is active.The SOD detection kit that this index measurement adopts Nanjing to build up Bioengineering Research Institute's production is carried out, and operating process is carried out according to the operation instruction of test kit.
(4) gene involved in immunity (lipopolysaccharide binding protein and antibacterial peptide gene) measures: the extraction by TRIZOL method, the hemocyte be stored in-80 DEG C being carried out to total serum IgE, RNA sample qualified for purity detecting is carried out reverse transcription synthesis cDNA, cDNA reverse transcription obtained carries out real-time fluorescence quantitative PCR detection, and real-time quantitative PCR data results adopts 2-Δ Δ Ct method to calculate the relative expression quantity of goal gene.
Testing data adopts the one-way analysis of variance (ONE-WAYANVOA) in SPSS18.0 to carry out statistical study, and average adopts Tukey method to carry out multiple comparisons, and test-results is in table 2.
Table 2
Note: * represents significant difference (p<0.05)
As can be seen from Table 2, bacillus cereus of the present invention can improve prawn cellular immunization, humoral immunization and related immune gene indices level, strengthens the immunizing power of prawn.
Embodiment 4
The present embodiment is used for the impact of bacillus cereus of the present invention on prawn opposing pathogenic bacterium ability is described.
By Vibrio harveyi, (Vibrioharveyi, purchased from ATCC, is numbered
mP-6
tM) be inoculated in 2216E liquid nutrient medium, cultivate 24h for 28 DEG C, then by 28 DEG C of cultivation 48h on liquid inoculation 2216E solid medium, scrape lawn, be diluted to 10 with sterile distilled water
7cfu/mL.After culture experiment terminates, from the treatment group and control group of embodiment 3, difference random selecting 60 tail Penaeus vannamei, puts into the Box of foamed plastics that 6 are equipped with 32L seawater, often organizes 3 repetitions.Each group of intramuscular injection 0.1mL Vibrio harveyi suspension (10
7cfu/mL).Arrange blank group, blank group injection stroke-physiological saline solution 0.1mL.The mortality ratio of continuous statistics 7d Penaeus vannamei.
Testing data adopts the one-way analysis of variance (ONE-WAYANVOA) in SPSS18.0 to carry out statistical study, and average adopts Tukey method to carry out multiple comparisons, and test-results is in table 3.
Table 3
| Group | Control group (%) | Treatment group (%) | Blank group |
| 1d | 11.67 | 8.33 | 0 |
| 2d | 25.00 | 26.67 | 0 |
| 3d | 26.67 | 35.00 | 0 |
| 4d | 31.67 | 35.00 | 0 |
| 5d | 35.00 | 41.67 | 0 |
| 6d | 38.33 | 41.67 | 0 |
| 7d | 43.33 | 41.67 | 0 |
As can be seen from the data of table 3, the mortality ratio for the treatment of group toxicity test is significantly lower than control group (particularly 7 days after mortality ratio).
Embodiment 5
Prawn is raised with reference to embodiment 2.After raising terminates, from treatment group and control group, random selecting 300 tail shrimp is weighed respectively, calculates rate of body weight gain (rate of body weight gain=[(body weight-original body mass in latter stage)/original body mass] × 100) and specific growth rate (specific growth rate=[(ln body weight in latter stage-ln original body mass)/number of days] × 100).
Testing data adopts the one-way analysis of variance (ONE-WAYANVOA) in SPSS18.0 to carry out statistical study, and average adopts Tukey method to carry out multiple comparisons, and test-results is in table 4.
Table 4
| Group | Original body mass | Latter stage body weight | Weightening finish | Rate of body weight gain | Specific growth rate |
| Control group | 4.55±0.15 | 6.81±0.021 | 2.26±0.02 | 49.63±0.51 | 1.44±0.012 |
| Treatment group | 4.55±0.15 | 7.92±0.021* | 3.37±0.02 | 73.93±0.42* | 1.98±0.008* |
Note: * represents significant difference (p<0.05)
As can be seen from Table 4, bacillus cereus of the present invention can significantly improve the speed of growth of prawn, thus Shortening culturing period.
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
It should be noted that in addition, each concrete technical characteristic described in above-mentioned embodiment, in reconcilable situation, can be combined by any suitable mode, in order to avoid unnecessary repetition, the present invention illustrates no longer separately to various possible array mode.
In addition, also can carry out arbitrary combination between various different embodiment of the present invention, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (5)
1. a bacillus cereus (Bacilluscereus), the preserving number of this bacillus cereus is CGMCCNo.9139.
2. a feed, is characterized in that, this feed contains bacillus cereus according to claim 1 and basal nutrient material.
3. feed according to claim 2, wherein, in the viable bacteria body of bacillus cereus, the content of described bacillus cereus in feed is 10
6-10
8cfu/g.
4. the feed according to Claims 2 or 3, wherein, described basal nutrient material comprises protein, lipid, carbohydrate, VITAMIN and mineral substance; With the gross weight of described feed for benchmark, the content of protein is 20-55 % by weight, the content of lipid is 15-20 % by weight, the content of carbohydrate is 20-35 % by weight, the content of VITAMIN is 1-1.5 % by weight and the content of mineral substance is 0.2-3 % by weight.
5. bacillus cereus according to claim 1 is preparing the purposes in prawn feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410203206.4A CN103966135B (en) | 2014-05-14 | 2014-05-14 | A kind of bacillus cereus and uses thereof and feed and uses thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410203206.4A CN103966135B (en) | 2014-05-14 | 2014-05-14 | A kind of bacillus cereus and uses thereof and feed and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103966135A CN103966135A (en) | 2014-08-06 |
| CN103966135B true CN103966135B (en) | 2016-03-09 |
Family
ID=51236143
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410203206.4A Active CN103966135B (en) | 2014-05-14 | 2014-05-14 | A kind of bacillus cereus and uses thereof and feed and uses thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103966135B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861389B (en) * | 2016-05-30 | 2019-06-28 | 天津师范大学 | For improving Bacillus strain and its screening technique and the application of aquatic livestock growth performance |
| CN111057661A (en) * | 2019-11-19 | 2020-04-24 | 浙江圣达生物药业股份有限公司 | 25-hydroxy vitamin D3Production strain and screening method and application thereof |
| CN111454867B (en) * | 2020-05-27 | 2022-04-01 | 吉林农业大学 | Phoxinus lagowskii waxy bacillus strain and application thereof |
| CN114145389B (en) * | 2021-12-24 | 2023-02-07 | 中国海洋大学 | Feed additive and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102028103A (en) * | 2010-12-12 | 2011-04-27 | 夏学德 | Microbial bait additive and preparation method thereof |
| CN103966134A (en) * | 2014-05-14 | 2014-08-06 | 北京鑫洋水产高新技术有限公司 | Mixed bacteria agent and use thereof, and feed and use thereof |
-
2014
- 2014-05-14 CN CN201410203206.4A patent/CN103966135B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102028103A (en) * | 2010-12-12 | 2011-04-27 | 夏学德 | Microbial bait additive and preparation method thereof |
| CN103966134A (en) * | 2014-05-14 | 2014-08-06 | 北京鑫洋水产高新技术有限公司 | Mixed bacteria agent and use thereof, and feed and use thereof |
Non-Patent Citations (1)
| Title |
|---|
| 蜡样芽孢杆菌对凡纳滨对虾幼体变态的影响;温崇庆等;《水产科学》;20070831;第26卷(第8期);第440-444页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103966135A (en) | 2014-08-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhou et al. | Role and functions of beneficial microorganisms in sustainable aquaculture | |
| Anand et al. | Effect of dietary supplementation of biofloc on growth performance and digestive enzyme activities in Penaeus monodon | |
| CN102399733B (en) | Lactobacillus johnsonii, microbial inoculum, application and premix thereof | |
| CN101485402B (en) | Composite biological feed additive agent for fattening early weaning mutton sheep | |
| Kumar et al. | Effect of dietary synbiotic on growth performance, body composition, digestive enzyme activity and gut microbiota in Cirrhinus mrigala (Ham.) fingerlings | |
| CN101638627B (en) | Bacillus subtilis and application thereof in biological feed additives | |
| CN102805209B (en) | Synbiotics feed additive for beasts and birds and application thereof | |
| Akter et al. | Effect of Lactobacillus acidophilussupplementation on growth performances, digestive enzyme activities and gut histomorphology of striped catfish (Pangasianodon hypophthalmusSauvage, 1878) juveniles | |
| CN103005159A (en) | Preparation method of ginkgo leaf biological feed additive | |
| CN101983582A (en) | Traditional Chinese medicine microecological preparation for preventing and treating enteritis of grass carp and preparation method thereof | |
| Olmos Soto | Feed intake improvement, gut microbiota modulation and pathogens control by using Bacillus species in shrimp aquaculture | |
| CN104480052A (en) | Composite bacillus preparation containing three strains, preparation method of composite bacillus preparation and application of composite bacillus preparation to ecological breeding | |
| CN101878858A (en) | Microecological composite growth-promoting feed additive for fish | |
| CN103053831A (en) | Compound premix feed of 1% green laying hens in laying period | |
| Saad et al. | Growth response of the freshwater prawn, Macrobrachium rosenbergii (De Man), to diets having different levels of Biogen® | |
| CN102283328A (en) | Multifunctional compound micro-ecological preparation for culturing holothurian | |
| CN106566791A (en) | Porcine compound synbiotics micro-ecological preparation and preparation method thereof | |
| CN107032504A (en) | Fisheries water quality improving agent and preparation method thereof | |
| Hadi et al. | Use of probiotic bacteria to improve growth and survivability of farmed New Zealand abalone (Haliotis iris) | |
| CN103966135B (en) | A kind of bacillus cereus and uses thereof and feed and uses thereof | |
| Wang et al. | Improving the quality of Laminaria japonica-based diet for Apostichopus japonicus through degradation of its algin content with Bacillus amyloliquefaciens WB1 | |
| Amir et al. | Evaluation of yeast and bacterial based probiotics for early rearing of Labeo rohita (Hamilton, 1822) | |
| CN108967742A (en) | A kind of compound micro-ecological preparation used for aquiculture and feed | |
| CN102517227B (en) | Enterococcus faecalis and applications and feed additive and leavening agent thereof | |
| CN105858909A (en) | Microbial herbal composite preparation for aquiculture and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231203 Address after: No. 118 Yimin West Road, Economic Development Zone, Dafeng District, Yancheng City, Jiangsu Province, 224000 Patentee after: Zhongke Xinyang (Jiangsu) Biotechnology Co.,Ltd. Address before: 102488, No.10 Jinguang Road, Liangxiang Industrial Development Zone, Fangshan District, Beijing Patentee before: BEIJING SEASUN AQUACULTURE BIOTECH CO.,LTD. |
|
| TR01 | Transfer of patent right |