CN108967742A - A kind of compound micro-ecological preparation used for aquiculture and feed - Google Patents
A kind of compound micro-ecological preparation used for aquiculture and feed Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/225—Faecalis
Abstract
The invention discloses a kind of compound micro-ecological preparations used for aquiculture, belong to the probiotics field of aquaculture technology.Compound micro-ecological preparation of the invention, including complex microorganism, compound enzyme system and complex immunity enhancement factor, the complex microorganism include enterococcus faecalis, enterococcus faecium, bacillus subtilis, S. cervisiae；The compound enzyme system includes protease, cellulase, lipase；The complex immunity enhancement factor includes manna oligosacchride, beta glucan, chitosan oligosaccharide, peptide glycan.Compound micro-ecological preparation provided by the invention can be effectively improved the stomach health problem occurred in current breeding process, strengthen immunity and disease-resistant anti-stress ability, escort for green and healthy cultivation.
Description
Technical field
The present invention relates to a kind of compound micro-ecological preparation used for aquiculture and feeds, belong to the Tiny ecosystem of aquaculture technology
Formulation art.
Background technique
Intensive in recent years, scale culture fishery rapid development, cultivation density continue to increase, breeding water body environment
Badly, various diseases frequently occur, all kinds of chemicals and abuse of antibiotics, resulting pathogenic bacteria resistance to drugs problem move
Flora imbalance and antibiotic residue problem are increasingly severe in object, so that antibiotic becomes global pollutant, it is huge
Big negative effect brings serious economic loss to culturist, brings fearful hidden danger to the food safety of consumer,
Hinder the sustainable development of world's aquaculture.What microorganism formulation itself had has no toxic side effect, noresidue, has no drug resistance
Feature makes biological control become the fundamental solution solved the problems, such as, and medical treatment disease can only be temporary means.Micro- life
After viable bacteria in object preparation enters in aquatic animal body, the micro-ecological environment inside and outside animal body can be repaired and be regulated and controled, restores machine
Body normal physiological function inhibits the generation of disease, promotes the growth of animal, enhances animal immune function.
Aquatic microorganisms preparation is also in the early stage of development at present, although research-and-development activity is increasingly deepened, application range
It is growing, but there are still some problems, such as the stability and compatibility problems of viable bacteria.
The application foundation of microorganism formulation is microecological balance, it is necessary to have sufficient amount of viable bacteria to can be only achieved dominant microflora
The purpose of foundation.Viable bacteria influence vulnerable to internal and external environment factor, such as temperature, pH value, dissolved oxygen etc..For complex microorganism system
Agent, be with two or more strain together, these strains and non-individual play a role, under certain condition, they
Between can also generate and influence each other, such as antagonism or inhibiting effect, to lose the meaning of cooperation.
Therefore, for aquatic livestock, develop that a kind of spawn activity is strong and the compound micro-ecological preparation of obvious effect is compeled
The eyebrows and eyelashes.
Summary of the invention
To solve the above-mentioned problems, the present invention provides a kind of compound micro-ecological preparation used for aquiculture.It is provided by the invention
Compound micro-ecological preparation can not only be effectively improved aquiculture animal stomach health problem, can also enhance immune, raising and resist
Sick anti-stress ability.
For this purpose, the invention provides the following technical scheme:
A kind of compound micro-ecological preparation that strong intestines food calling enhancing used for aquiculture is immune, contains complex microorganism, complex enzyme
System and complex immunity enhancement factor.The complex microorganism includes enterococcus faecalis, enterococcus faecium, bacillus subtilis, wine
Brewer yeast bacterium；The compound enzyme system includes protease, cellulase, lipase；The complex immunity enhancement factor packet
Include manna oligosacchride, beta glucan, chitosan oligosaccharide, peptide glycan.
Further, by weight, including enterococcus faecalis 50~150, enterococcus faecium 1~9, bacillus subtilis 5~35,
S. cervisiae 5~25, proteinase-10 .1~0.9, cellulase 0.02~0.18, lipase 0.02~0.18, manna oligosacchride
0.05~0.35, beta glucan 0.02~0.18, chitosan oligosaccharide 0.02~0.18, peptide glycan 0.02~0.18.
Further, by weight, including enterococcus faecalis 80~120, enterococcus faecium 3~7, bacillus subtilis 10~
30, S. cervisiae 10~20, proteinase-10 .3~0.7, cellulase 0.05~0.15, lipase 0.05~0.15, sweet dew widow
Sugar 0.1~0.3, beta glucan 0.05~0.15, chitosan oligosaccharide 0.05~0.15, peptide glycan 0.05~0.15.
Further, each component parts by weight of the microorganism formulation are as follows: enterococcus faecalis 100, enterococcus faecium 5,
Bacillus subtilis 20, S. cervisiae 15, proteinase-10 .5, cellulase 0.1, lipase 0.1, manna oligosacchride 0.2, β-Portugal
Glycan 0.1, chitosan oligosaccharide 0.1, peptide glycan 0.1.
Wherein, the viable bacteria content of each microorganism is as follows, and enterococcus faecalis viable bacteria content is not less than 1 × 109Cfu/g, dung intestines ball
Bacterium viable bacteria content is not less than 1 × 108Cfu/g, bacillus subtilis viable bacteria content are not less than 1 × 108Cfu/g, S. cervisiae are living
Bacterial content is not less than 1 × 108cfu/g。
In a further embodiment of the present invention, the compound micro-ecological preparation is used for mixed feed and is used, and every kg
Compound micro-ecological preparation described in 2~5g of feed addition；Or compound micro-ecological preparation is added in 40~50 times of water and activates 4~6
After hour, full pool spilling head is used, and usage amount is 100~250g/ mus of rice.
The immune compound micro-ecological preparation of the dedicated strong intestines food calling enhancing of aquatic livestock of the present invention, with enterococcus faecalis,
Enterococcus faecium, bacillus subtilis, S. cervisiae provide abundant nutrition substance for aquatic livestock, mention simultaneously as probiotics
High resistance Gram-negative bacteria ability, improves intestine microbial diversity；Using protease, cellulase, lipase as enzyme
System provides digestive ferment for the enteron aisle of aquatic livestock, promotes the digestion and absorption function to feed；It joined manna oligosacchride, β-
Glucan, chitosan oligosaccharide, peptide glycan enhance aquatic livestock immune function, and body is made to generate the antibody level of Gao Erqiang, enhance machine
Body disease resistance, hence it is evident that the immune function for promoting aquatic livestock significantly improves aquatic livestock growth rate.
In the compound micro-ecological preparation, enterococcus faecalis and enterococcus faecium belong to genus lactubacillus, and lactic acid bacteria is animal intestinal tract
Interior normal flora is colonized in enteron aisle, can produce natural antibiotic, be conducive to body health, can also generate to thin
Bacterium etc. has the substance of inhibiting effect, inhibits the growth of pathogen, improves intestinal microenvironment.Bacillus subtilis is during the growth process
Subtilin, polymyxins isoreactivity substance, which can be generated, has apparent inhibiting effect to pathogen or conditioned pathogen；In addition its
The free oxygen in digestive tract environment can be consumed rapidly, to form enteron aisle low-oxygen environment, promote to grow beneficial to anaerobic bacteria, and produce
The organic acids such as lactogenesis acid reduce intestinal pH, inhibit the growth of pathogenic bacteria indirectly；Bacillus subtilis can in animal intestinal tract generation
It thanks, be proliferated, adjust intestinal flora, improve body digestion power, strengthen immunity and premunition, multivitamin can also be synthesized
Nutriment is improved food conversion ratio.Saccharomyces cerevisiae cells are rich in protein, nucleic acid, vitamin and a variety of enzymes, have enhancing
Animal immunizing power increases feed palatability, improves animal to the abilities of digestive and absorption of feed.
Enzyme system can supplement the deficiency of endogenous enzymes, improve the activity of endogenous digestive ferment, reduce the increase of harmful substance, change
Become gut wall structure, improves the absorbability to nutrient.Enzyme can digest the nutriment wrapped up originally by cell wall structure, increase
The utilization rate of polysaccharide, grease and protein etc. in feed.A certain proportion of complex enzyme formulation is added in feed, can effectively be changed
Kind digestion and absorption of the animal to the vegetal cells wall material such as cellulose, hemicellulose, pectin in feed, improves to protein
With the utilization of the nutriments such as starch, to improve weight gain and the efficiency of feed utilization of animal.Protease can promote aquatic livestock pair
The digestion of protein generates the saving effect to protein, improves protein efficiency ratio, PER, promotes growth of animal.Lipase function is
It is monoglyceride, diglyceride and free fatty acid by fat splitting, is a kind of intracorporal important digestive enzymes of animal.
Immune-enhancing effect factor manna oligosacchride, beta glucan, chitosan oligosaccharide, peptide glycan can enhance the immune function of aquatic livestock.
Manna oligosacchride can promote the proliferation of hindgut beneficial bacterium, and the general level of the health for improving animal reaches regulator function；In addition, passing through promotion
The excretion of harmful bacteria, immunologic adjuvant and the approach such as activation animal specificity is immune, improve body entirety immune function, prevent disease
Generation.Beta glucan forms spiral molecular structure because of the presence of its special bond structure mode and intramolecular hydrogen bond, this
The special configuration of kind, it is easy to be received by immune system.Beta glucan can improve the growth of aquatic livestock significantly, be immunized
The immune indexes such as protection and phagocytic index, total hemocytes count.The proliferation and cell factor that chitosan oligosaccharide passes through adjusting immunocyte
Release realize enhancing immunity of organism；The quantity of pernicious bacteria in enteron aisle can be reduced, the number of profitable strain in enteron aisle is increased
Amount improves intestinal environment, promotes intestinal flora balance；The synthesis and metabolism of protein can also be influenced, other substances of body are adjusted
Metabolism, the apparent digestibility and utilization rate of feed are improved, to promote growth of animal.Peptide glycan is present in gram-positive bacteria
In gram-negative bacteria cell wall, it is the disaccharides formed by N-Acetyl-D-glucosamine and -acetylmuramic acid, passes through peptide chain phase
Mutually intersect the polymer constituted.Suitable peptide glycan can significantly improve aquatic livestock serum phenol oxidase, acidity and alkaline phosphorus
The salivary lysozymes such as sour enzyme, lysozyme.
Above-mentioned technical proposal provided by the invention has the advantage that
Lactic acid bacteria microbe selected by compound micro-ecological preparation of the invention (enterococcus faecalis and enterococcus faecium) uses coating
Technique production can promote to add the effects of can greatly improving bacterial viability, resist hydrochloric acid, bile acid in alimentary canal
Beneficial bacterium can be colonized for a long time enteron aisle and function.In addition complex microorganism preparations of the invention selection has synergistic effect
Bacillus and lactic acid bacteria carry out it is reasonably combined.Bacillus is height aerobic bacteria, and forming internal anaerobic environment can effectively press down
Pathogenic bacteria growth processed, while being conducive to the lactobacter growth of anaerobism again, lactobacter growth increases the acidity of environment, further increases
By force to the inhibiting effect of pathogenic bacteria.Compound enzyme system and the Immune-enhancing effect factor in compound micro-ecological preparation of the present invention, after use
Aquatic livestock can be improved to the abilities of digestive and absorption of feed, promote growth, improve its immune disease-resistance ability.
Detailed description of the invention
Fig. 1 show test example 5 and tests filefish intestine colibacillosis eucaryotic cell structure in preceding pond.
Fig. 2 show after test example 5 is tested filefish intestine colibacillosis eucaryotic cell structure in pond.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
Embodiment 1
A kind of compound micro-ecological preparation used for aquiculture enhances containing complex microorganism, compound enzyme system and complex immunity
The factor.Complex microorganism includes 50 parts of enterococcus faecalis, 8 parts of enterococcus faecium, 20 parts of bacillus subtilis, 20 parts of S. cervisiae；
Compound enzyme system includes .5 parts of proteinase-10,0.15 part of cellulase, 0.15 part of lipase；Complex immunity enhancement factor includes sweet
0.3 part of oligosaccharides, 0.15 part of beta glucan, 0.15 part of chitosan oligosaccharide, 0.15 part of peptide glycan of dew.
The viable bacteria content of each microorganism of compound micro-ecological preparation is as follows, and enterococcus faecalis viable bacteria content is not less than 1 × 109cfu/
G, enterococcus faecium viable bacteria content is not less than 1 × 108Cfu/g, bacillus subtilis viable bacteria content are not less than 1 × 108Cfu/g, wine brewing
Saccharomycete viable bacteria content is not less than 1 × 108cfu/g。
Embodiment 2
A kind of compound micro-ecological preparation used for aquiculture enhances containing complex microorganism, compound enzyme system and complex immunity
The factor.Complex microorganism includes 100 parts of enterococcus faecalis, 3 parts of enterococcus faecium, 30 parts of bacillus subtilis, 20 parts of S. cervisiae；
Compound enzyme system includes .5 parts of proteinase-10,0.15 part of cellulase, 0.15 part of lipase；Complex immunity enhancement factor includes sweet
0.2 part of oligosaccharides, 0.15 part of beta glucan, 0.15 part of chitosan oligosaccharide, 0.15 part of peptide glycan of dew.
The viable bacteria content of each microorganism of compound micro-ecological preparation is as follows, and enterococcus faecalis viable bacteria content is not less than 1 × 109cfu/
G, enterococcus faecium viable bacteria content is not less than 1 × 108Cfu/g, bacillus subtilis viable bacteria content are not less than 1 × 108Cfu/g, wine brewing
Saccharomycete viable bacteria content is not less than 1 × 108cfu/g。
Embodiment 3
A kind of compound micro-ecological preparation used for aquiculture enhances containing complex microorganism, compound enzyme system and complex immunity
The factor.Complex microorganism includes 120 parts of enterococcus faecalis, 5 parts of enterococcus faecium, 30 parts of bacillus subtilis, 10 parts of S. cervisiae；
Compound enzyme system includes .4 parts of proteinase-10,0.05 part of cellulase, 0.05 part of lipase；Complex immunity enhancement factor includes sweet
0.1 part of oligosaccharides, 0.15 part of beta glucan, 0.15 part of chitosan oligosaccharide, 0.15 part of peptide glycan of dew.
The viable bacteria content of each microorganism of compound micro-ecological preparation is as follows, and enterococcus faecalis viable bacteria content is not less than 1 × 109cfu/
G, enterococcus faecium viable bacteria content is not less than 1 × 108Cfu/g, bacillus subtilis viable bacteria content are not less than 1 × 108Cfu/g, wine brewing
Saccharomycete viable bacteria content is not less than 1 × 108cfu/g。
Embodiment 4
A kind of compound micro-ecological preparation used for aquiculture enhances containing complex microorganism, compound enzyme system and complex immunity
The factor.Complex microorganism includes 150 parts of enterococcus faecalis, 5 parts of enterococcus faecium, 10 parts of bacillus subtilis, 15 parts of S. cervisiae；
Compound enzyme system includes .2 parts of proteinase-10,0.18 part of cellulase, 0.18 part of lipase；Complex immunity enhancement factor includes sweet
0.1 part of oligosaccharides, 0.1 part of beta glucan, 0.1 part of chitosan oligosaccharide, 0.1 part of peptide glycan of dew.
The viable bacteria content of each microorganism of compound micro-ecological preparation is as follows, and enterococcus faecalis viable bacteria content is not less than 1 × 109cfu/
G, enterococcus faecium viable bacteria content is not less than 1 × 108Cfu/g, bacillus subtilis viable bacteria content are not less than 1 × 108Cfu/g, wine brewing
Saccharomycete viable bacteria content is not less than 1 × 108cfu/g。
Embodiment 5
A kind of compound micro-ecological preparation used for aquiculture enhances containing complex microorganism, compound enzyme system and complex immunity
The factor.Complex microorganism includes 100 parts of enterococcus faecalis, 5 parts of enterococcus faecium, 10 parts of bacillus subtilis, 15 parts of S. cervisiae；
Compound enzyme system includes .1 parts of proteinase-10,0.1 part of cellulase, 0.1 part of lipase；Complex immunity enhancement factor includes sweet dew
0.1 part of oligosaccharides, 0.1 part of beta glucan, 0.1 part of chitosan oligosaccharide, 0.1 part of peptide glycan.
The viable bacteria content of each microorganism of compound micro-ecological preparation is as follows, and enterococcus faecalis viable bacteria content is not less than 1 × 109cfu/
G, enterococcus faecium viable bacteria content is not less than 1 × 108Cfu/g, bacillus subtilis viable bacteria content are not less than 1 × 108Cfu/g, wine brewing
Saccharomycete viable bacteria content is not less than 1 × 108cfu/g。
Embodiment 6
A kind of compound micro-ecological preparation used for aquiculture enhances containing complex microorganism, compound enzyme system and complex immunity
The factor.Complex microorganism includes 100 parts of enterococcus faecalis, 5 parts of enterococcus faecium, 20 parts of bacillus subtilis, 15 parts of S. cervisiae；
Compound enzyme system includes .5 parts of proteinase-10,0.1 part of cellulase, 0.1 part of lipase；Complex immunity enhancement factor includes sweet dew
0.2 part of oligosaccharides, 0.1 part of beta glucan, 0.1 part of chitosan oligosaccharide, 0.1 part of peptide glycan.
The viable bacteria content of each microorganism of compound micro-ecological preparation is as follows, and enterococcus faecalis viable bacteria content is not less than 1 × 109cfu/
G, enterococcus faecium viable bacteria content is not less than 1 × 108Cfu/g, bacillus subtilis viable bacteria content are not less than 1 × 108Cfu/g, wine brewing
Saccharomycete viable bacteria content is not less than 1 × 108cfu/g。
Test example 1
Carp culture test selection Haikang base carry out, choose the same cement pit temporarily support a week carp into
Row formal test.To select physically fit, 825 tail of carp (10g or so) of the same size is randomly divided into 11 groups, every group of 3 repetitions,
25 tail carps are put in each repetition in a suitable place to breed, and test is 300L with circulation barrel water capacity.Three time points of selection be satiated with food feeding daily:
8:00,12:00,19:00.Claim a feed during cultivation weekly, calculates feed total amount of ingesting weekly, feed end every morning
After change water, quantity of exchanged water 1/2 or so, the culture-cycle is 8 weeks.Control group fed routine business feed.Test group I is feeding conventional quotient
It is mixed when industry feed and feeds enterococcus faecalis and enterococcus faecium.Test group II is mixed when feeding routine business feed feeds bacillus subtilis.
Test group III is mixed when feeding routine business feed feeds S. cervisiae.Test group IV mixed when feeding routine business feed feed it is thermophilic
Lactobacillus lactis.Test group V is mixed when feeding routine business feed feeds bacillus licheniformis.Test group VI is in feeding routine business
It is mixed when feed and feeds Candida.Test group VII is mixed when feeding routine business feed feeds enterococcus faecalis, enterococcus faecium and withered grass bud
Spore bacillus.Test group VIII is mixed when feeding routine business feed feeds enterococcus faecalis, enterococcus faecium and S. cervisiae.Test group Ⅸ
It is mixed when feeding routine business feed and feeds bacillus subtilis and S. cervisiae.Test group Ⅹ is when feeding routine business feed
It mixes and feeds enterococcus faecalis, enterococcus faecium, bacillus subtilis and S. cervisiae.The viable count of various bacterium used above guarantees not low
In 1 × 108Cfu/g is wherein right in enterococcus faecalis, enterococcus faecium, bacillus subtilis and saccharomyces cerevisiae bacterial content and embodiment 6
Each bacterial content answered is consistent.The content of VI each group bacterium of test group IV, test group V and test group respectively with test group I, examination
It is consistent with the content of the corresponding each bacterium of test group III to test group II.
Influence of the complex microorganism of the present invention of table 1 to carp growth performance
Influence of the complex microorganism of the present invention of table 2 to Serum of Common Carp salivary lysozyme
As can be seen from Table 1, using complex microorganism of the invention can significantly improve the rate of body weight gain of carp, grazing rate,
Feed efficiency and survival rate.As can be seen from Table 2, the bacteriolyze of carp can be significantly improved using complex microorganism of the invention
The vigor of enzyme, superoxide dismutase and Complement C_3.This explanation can not only be obviously promoted taking the photograph for carp using complex microorganism
Food and growth can also significantly improve the immunity of carp.
Test example 2
The selection of perch culture experiment carries out in Haikang base, chooses the fry that the same cement pit has temporarily been supported one month and carries out
Formal test.Select physically fit, 675 tail of perch (11g or so) of the same size is randomly divided into 9 groups, every group of 3 repetitions, often
25 tail carps are put in a repetition in a suitable place to breed, and test is 300L with circulation barrel water capacity.Two time points of selection be satiated with food feeding daily: 8:
00,17:30.Claim a feed during cultivation weekly, calculate feed total amount of ingesting weekly, change water after feeding every morning,
Quantity of exchanged water 2/3 or so, culture-cycle are 8 weeks.Control group fed routine business feed.Test group I is in feeding routine business feed
When mix feed chitosan oligosaccharide and manna oligosacchride.Test group II is mixed when feeding routine business feed feeds beta glucan and peptide glycan.Test
Group III is mixed when feeding routine business feed feeds chitosan oligosaccharide and beta glucan.Test group IV is mixed when feeding routine business feed feeds
Manna oligosacchride and peptide glycan.Test group V is mixed when feeding routine business feed feeds galactomannan-oligosaccharide and chitosan.Test group
VI mixes hello chitosan oligosaccharide, manna oligosacchride and beta glucan when feeding routine business feed.Test group VII is raised in feeding routine business
It is mixed when material and feeds chitosan oligosaccharide, beta glucan and peptide glycan.Test group VIII is mixed when feeding routine business feed feeds chitosan oligosaccharide, sweet dew widow
Sugar, beta glucan and peptide glycan.The content and implementation of each immune polysaccharide of the above manna oligosacchride, beta glucan, chitosan oligosaccharide, peptide glycan
Corresponding each immune polysaccharide content is consistent in example 6.V galactomannan-oligosaccharide of test group and chitosan mix the amount of feeding and test
Group IV is consistent.
Influence of the complex immunity enhancement factor of the present invention of table 3 to perch growth performance
Influence of the complex immunity enhancement factor of the present invention of table 4 to perch serum non-specific immunity index
As can be seen from Table 3, the rate of body weight gain of perch can be significantly improved using complex immunity enhancement factor of the invention, take the photograph
Food rate, feed efficiency and survival rate.As can be seen from Table 4, it can be significantly improved using complex immunity enhancement factor of the invention
The vigor of the lysozyme of perch, superoxide dismutase and alkaline phosphatase.This explanation uses complex immunity enhancement factor not only
Be conducive to improve ingesting and growing for perch, be more advantageous to the immunity for improving perch.
Test example 3
The selection of litopenaeus vannamei culture experiment carries out in Haikang base, chooses the shrimp that the same cement pit has temporarily been supported one month
Seedling carries out formal test.Select that size is uniform, body colour and good 1200 tail of healthy Juvenile Litopenaeus vannamei (1.5g or so) of vigor
8 groups are randomly divided into, 50 tail seedling are put in every group of 3 repetitions, each repetition in a suitable place to breed, and test is 300L with circulation barrel water capacity.Choosing daily
Selecting three time points is fed: 8:00,14:30,19:30, the health status of each breeding barrel Prawn of observation before feeding every time
And shell situation, the 10% of cultivation daily ration, feeding quantity early period percentage of liveweight, the later period, daily ration, feeding quantity was down to 4% with the increase of prawn weight, directly
To off-test.Claim a feed during cultivation weekly, calculates feed total amount of ingesting weekly, changed after feeding every morning
Water, quantity of exchanged water 1/3 or so, culture-cycle are 8 weeks.The wherein refreshing prawn mixed feed 9102 of control group fed mind, does not mix and feeds this hair
Bright compound microecological microbial inoculum product.Test group I is to mix to feed product of the present invention in the refreshing prawn mixed feed 9102 of feeding mind
Fall compound enzyme system and complex immunity enhancement factor.Test group II is to mix to feed this hair in the refreshing prawn mixed feed 9102 of feeding mind
Bright product removes complex microorganism and complex immunity enhancement factor.Test group III is in the refreshing prawn mixed feed 9102 of feeding mind
It mixes hello product of the present invention and removes complex microorganism and compound enzyme system.Test group IV is in the refreshing prawn mixed feed 9102 of feeding mind
When mix feed product of the present invention remove compound enzyme system.Test group V is to mix to feed this hair in the refreshing prawn mixed feed 9102 of feeding mind
Bright product removes complex immunity enhancement factor.Test group VI is to mix hello the present invention in the refreshing prawn mixed feed 9102 of feeding mind to produce
Product remove complex microorganism.Test group VII mixes in the refreshing prawn mixed feed 9102 of feeding mind and feeds product of the present invention.The present invention produces
Product are the product prepared according to embodiment 6.
Refreshing 9102 dietary nutritional standard of prawn mixed feed of 5 mind of table
| Crude protein | Crude fat | Crude fibre | Coarse ash | Moisture | Total phosphorus | Lysine |
| 43.0 | 6.0 | 4.0 | 15.0 | 12.0 | 2.0 | 2.3 |
Influence of the compound microecological microbial inoculum product of the present invention of table 6 to Growth of Litopenaeus vannamei performance
As can be seen from Table 6, using the litopenaeus vannamei rate of body weight gain of compound micro-ecological preparation product of the invention, specific life
Long rate and survival rate all highests, feed coefficient are minimum.Illustrating the compound micro-ecological preparation product of the invention not only can be improved pair
Utilization of the shrimp to feed, promotes the growth of prawn, can also increase the resistance of prawn, improves its survival rate.
Test example 4 is cultivated applied to litopenaeus vannamei
Compound micro-ecological preparation made from Example 1~6, and be used in litopenaeus vannamei cultivating pool: design
Control group and experimental group, total 7 groups, every group of 3 repetitions.Amount addition cooperation in experimental group in prawn feed according to 2g/kg makes
With control group is blank control, that is, feeds identical feed but do not add compound micro-ecological preparation；After test 5 months, cultivation is calculated
The feed coefficient and survival rate of each group prawn in.
As can be seen from Table 7, being all apparently higher than using the survival rate of the test group of compound micro-ecological preparation of the present invention is not made
With the control group of compound micro-ecological preparation of the present invention, and feed coefficient is substantially less than control group.Illustrate compound micro- using the present invention
The survival rate of litopenaeus vannamei can be improved in ecological agent, reduces its feed coefficient.
Influence of the compound microecological microbial inoculum product of the present invention of table 7 to Growth of Litopenaeus vannamei performance
Test example 5
River Puffer feeding habits are violent, very gluttonous, and 15 minutes after generally feeding, feed is just eaten habit with nothing left, gluttonous by river Puffer
Property to many pressure of river Puffer enteron aisle band, so to growth peak period, discovery river Puffer have slight enteritis, intestinal mucosa epithelium
It falls off, intestinal villus film is sparse, and yellow mucus is relatively more, and enteron aisle poor toughness (uses promesenteron intestine colibacillosis structure such as Fig. 1).
It is applied to pond according to the formula of embodiment 6, mixed feed feeds river Puffer after mixing evenly, and dosage is 5g/kg feed,
Continuous use 7 days.
Using intestines in one section after a week, are cut off, it is found that intestines toughness becomes strong in the Puffer of river, is difficult to spread out on slide, inner surface
Clean and tidy no faint yellow mucus of discovery, inner surface chorion densification are thick and solid (middle intestines intestine colibacillosis structure such as Fig. 2 after use).It uses
Compound micro-ecological preparation of the invention not only solves river Puffer enteritis problem, reduces the use of enteritis drug, and enteron aisle is more strong
Kang Liao, we, which can trust, courageously feeds, and river Puffer, which has been digested and assimilated, not only reduces feed coefficient, reduces feed cost, and
And may also speed up the long speed of river Puffer, shorten the culture-cycle, lists as early as possible.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the technical principles of the invention, several improvements and modifications can also be made, these improvements and modifications
Also it should be regarded as protection scope of the present invention.
Claims (6)
1. a kind of compound micro-ecological preparation that strong intestines food calling enhancing used for aquiculture is immune, which is characterized in that including composite microbial
Object, compound enzyme system and complex immunity enhancement factor, the complex microorganism include enterococcus faecalis, enterococcus faecium, withered grass bud
Spore bacillus, S. cervisiae；The compound enzyme system includes protease, cellulase, lipase；The complex immunity increases
The strong factor includes manna oligosacchride, beta glucan, chitosan oligosaccharide, peptide glycan.
2. compound micro-ecological preparation according to claim 1, which is characterized in that by weight, including enterococcus faecalis 50~
150, enterococcus faecium 1~9, bacillus subtilis 5~35, S. cervisiae 5~25, proteinase-10 .1~0.9, cellulase
0.02~0.18, lipase 0.02~0.18, manna oligosacchride 0.05~0.35, beta glucan 0.02~0.18, chitosan oligosaccharide 0.02
~0.18, peptide glycan 0.02~0.18.
3. compound micro-ecological preparation according to claim 2, which is characterized in that by weight, including enterococcus faecalis 80
~120, enterococcus faecium 3~7, bacillus subtilis 10~30, S. cervisiae 10~20, proteinase-10 .3~0.7, cellulose
Enzyme 0.05~0.15, lipase 0.05~0.15, manna oligosacchride 0.1~0.3, beta glucan 0.05~0.15, chitosan oligosaccharide 0.05
~0.15, peptide glycan 0.05~0.15.
4. compound micro-ecological preparation according to claim 3, which is characterized in that each component weight of the microorganism formulation
It is as follows to measure number: enterococcus faecalis 100, enterococcus faecium 5, bacillus subtilis 20, S. cervisiae 15, proteinase-10 .5, cellulose
Enzyme 0.1, lipase 0.1, manna oligosacchride 0.2, beta glucan 0.1, chitosan oligosaccharide 0.1, peptide glycan 0.1.
5. compound micro-ecological preparation according to claim 1, which is characterized in that the viable bacteria content of each microorganism is as follows, excrement intestines
Coccus viable bacteria content is not less than 1 × 109Cfu/g, enterococcus faecium viable bacteria content are not less than 1 × 108Cfu/g, bacillus subtilis are living
Bacterial content is not less than 1 × 108Cfu/g, S. cervisiae viable bacteria content are not less than 1 × 108cfu/g。
6. the feed containing the described in any item compound micro-ecological preparations of claim 1-5, which is characterized in that add in every kg feed
Add the 2~5g of compound micro-ecological preparation.
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| CN111903836A (en) * | 2020-05-26 | 2020-11-10 | 广东海洋大学 | Feed additive for improving high-temperature stress resistance of marine cultured animals and preparation method and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110338275A (en) * | 2019-08-05 | 2019-10-18 | 通威股份有限公司 | A kind of extrusion type biological fermentation feed and its preparation and application method |
| CN110607258A (en) * | 2019-09-09 | 2019-12-24 | 武汉新华扬生物股份有限公司 | Biological agent for enzymolysis of coconut meal and application method thereof |
| CN110607258B (en) * | 2019-09-09 | 2021-07-02 | 武汉新华扬生物股份有限公司 | Biological agent for enzymolysis of coconut meal and application method thereof |
| CN111903836A (en) * | 2020-05-26 | 2020-11-10 | 广东海洋大学 | Feed additive for improving high-temperature stress resistance of marine cultured animals and preparation method and application thereof |
| CN115836708A (en) * | 2021-12-08 | 2023-03-24 | 中国水产科学研究院东海水产研究所 | River crab intestinal microbial diversity regulation feed and preparation and application thereof |
| CN114631602A (en) * | 2022-04-02 | 2022-06-17 | 江西省科学院生物资源研究所 | Application and method of chitosan oligosaccharide in improving application effect of chitosan microorganism composite preparation |
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Application publication date: 20181211 |