CN104664169B - A kind of ruminant feed bio-enhancer and preparation method thereof - Google Patents
A kind of ruminant feed bio-enhancer and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of ruminant feed bio-enhancer and preparation method thereof, it is comprised the following steps：A) fermentation medium is prepared, by weight：Carbon source is 85% 95%, nitrogen source is 1% 2%, growth promoter is 1% 3%, balance of water, mentioned component is crushed, mixing, standby；B) candida utili, solid fermentation culture are inoculated with step a) gained fermentation mediums；C) the inoculated plant lactobacillus in step b) gained fermentation mediums, anaerobic solids fermented and cultured, the sampling detection OD per 4h600；D) OD is treated600Into exponential phase, bacillus subtilis, solid fermentation culture are inoculated with step c) gained fermentation mediums.Biological feedstuff prepared by this method can improve ruminant tumor gastric microecological balance, improve food conversion ratio and immunity of organisms, and product has a kind of special sour-sweet smell, and good palatability, animal is easy to feed.
Description
Technical field
The invention belongs to ruminant tumor gastric technical field of microbe application, and in particular to ruminant feed Bioaugnentation
Agent and preparation method thereof.
Background technology
With developing rapidly for milk cow production and agent for rearing and fattening meat sheep industry, breeding performonce fo animals are continuously increased, antibiotic it is wide
It is general to use, cause animal body autogenous infection, drug resistance to produce；In cud beneficial microbe encroached on, it is dead or with
Digestion food is excreted, and is destroyed the microecological balance of normal flora in animal intestinal tract, and breeding performonce fo animals decline, from
And have influence on the economic benefit and social benefit of aquaculture.
Using ruminant tumor gastric microecological balance theory and technology, multiple beneficial microculture is prepared into complex microorganism
Microbial inoculum, is applied in ruminant animal nutrition, can play supplement micro organism quantity, maintains cud microecological balance；Have in cud
A large amount of vitamins, active material that beneficial microorganism metabolism is produced, can promote animal digestion function of stomach and intestine；The sugar of Microbe synthesis
The materials such as class, amino acids, for animal growth provides high-quality nutriment.Microbial metabolic products digestion in cud
The complexity such as starch, fat, protein, cellulose larger molecular organicses in the degradable feed of enzyme, change into animal and are easy to digestion suction
The small organic molecules such as the monosaccharide and disaccharide and amino acid of receipts.
It is active bacteria formulation and its fermentation culture medium to be studied in ruminant animal nutrition more both at home and abroad at present.Active bacteria formulation
General to be prepared by vitro culture by saccharomyces cerevisiae single bacterial strain, Main Function is for cud provides living cells and fermentating metabolism is produced
Thing, with ruminal digestion is promoted, improves efficiency of feed utilization and conversion ratio function.Fermentation culture medium is by external training by microorganism
Prepared by hair care ferment, it is used to promote animal digestion function with enzymatic activity material.
But also there are various problems in current fermentation process and tunning：
1) current similar product is all that drying temperature is too high to be easily caused bacterial strain inactivation, and dries temperature by dried process
Degree low then drying time is long, expends the energy.
Although 2) compound bacteria-fermented of bacillus, lactic acid bacteria and saccharomycete is used in like product at present more, by
It is different in its fermentation condition, while fermentation can produce influence each other.
3) in order to increase the intake of fermentate, it is necessary to add the bio-enhancer of excessive fermentation in feed, and then
PH value in influence ruminant tumor gastric.
4) product viable bacteria content is unstable and price is high-leveled and difficult with popularization.
The content of the invention
In order to solve the above mentioned problem of prior art presence, the invention provides a kind of biological increasing of ruminant biological feedstuff
The preparation method of strong agent, what this method was produced mainly for the distinctive ruminal digestion function of ruminant both have is supplemented with
Beneficial microorganism quantity adjusts cud microecological balance, and can provide that viable bacteria and metabolite promote body cell breeding again ruminates dynamic
Thing lumen fermentation adjusting function biological feedstuff bio-enhancer.
A scheme of the invention is related to a kind of preparation method of ruminant feed bio-enhancer, and it includes following step
Suddenly：
A) fermentation medium is prepared, by weight：Carbon source is 85%-95%, nitrogen source is 1%-2.0%, growth promoter
It is 1%-3%, mentioned component is crushed, mixed by balance of water, standby；
B) candida utili (ATCC 22023), solid fermentation culture are inoculated with step a) gained fermentation mediums；
C) the inoculated plant lactobacillus (ATCC 8014) in step b) gained fermentation mediums, anaerobic solids fermented and cultured,
Every 4 hours sampling detection OD600；
D) OD is treated600Into exponential phase, bacillus subtilis (ATCC is inoculated with step c) gained fermentation mediums
6633), solid fermentation culture.
Preferably, carbon source is that 91%, nitrogen source is that 1%, growth promoter is 2% and water is 6% in step a).
Preferably, after fermentation is fully completed, not comprising dry step.
Further, carbon source described in step a) is maize peel, rice bran, wheat bran, corn flour；Preferably described corn
Skin, rice bran, wheat bran, the part by weight of corn flour are 15-25：20-30：30-40：7-15.More preferably described maize peel, rice
Chaff, wheat bran, the part by weight of corn flour are 20：25：35：11.
Further, candida utili described in step b) to be to treat that the inoculum concentration that fermentation medium is counted is 8%-12%,
35-40 DEG C of fermented and cultured 20-28h.Preferably, the candida utili is to treat that the inoculum concentration that fermentation medium is counted is 9%-
11%, 36-38 DEG C of fermented and cultured 22-26h.It is highly preferred that inoculum concentration of the candida utili in terms for the treatment of fermentation medium
It is 10%, 37 DEG C of fermented and cultured 24h.
Further, Lactobacillus plantarum described in step c) is to treat that the inoculum concentration that fermentation medium is counted is 4%-8%, 25-
35 DEG C of fermented and cultured 20-28h.Preferably, the candida utili to be to treat that the inoculum concentration that fermentation medium is counted is 5%-7%,
28-32 DEG C of fermented and cultured 20-24h.It is highly preferred that the candida utili is to treat that the inoculum concentration that fermentation medium is counted is 6%,
30 DEG C of fermented and cultured 20-24h.
Further, bacillus subtilis described in step d) to be to treat that the inoculum concentration that fermentation medium is counted is 2%-6%,
28-37 DEG C of fermented and cultured 20-28h.Preferably, the candida utili is to treat that the inoculum concentration that fermentation medium is counted is 3%-
5%, 28-32 DEG C of fermented and cultured 22-26h.It is highly preferred that candida utili inoculum concentration in terms for the treatment of fermentation medium is
4%, 33 DEG C of fermented and cultured 24h.
Further, directly packed through pellet rotary screen after fermentation ends.
Yet another aspect of the present invention is related to a kind of ruminant feed bio-enhancer, and it is prepared by the above method.
Another aspect of the present invention is related to a kind of ruminant feed, the feed to include foregoing bio-enhancer.
Further, with the gross weight meter of feed, bio-enhancer 1%-50% can be included in the feed.It is preferred that wrapping
Containing 5%-30%, more preferably 10%-30%.
Further, in the feed comprising bio-enhancer 8-13 parts as claimed in claim 7, corn 30-50 parts,
Wheat bran 10-25 parts, soya-bean cake 5-20 parts, cottonseed cake 5-20 parts, salt 1.2-1.8 parts, soda 1.5-2.3 parts, stone flour 0.8-1.5
Part, bone meal 0.5-1.2 parts, calcium carbonate 0.5-1.2 parts.
There is candida utili bacterium viable count to be more than 1 × 10 in product9cfu/g；Lactobacillus plantarum viable count more than 1 ×
108cfu/g；Bacillus subtilis viable count is more than 1 × 1010cfu/g；Crude protein content is more than 20%.It is anti-that this method is produced
, 15% or so, with the special sour-sweet smell of one kind, good palatability, animal is easy for hay animal organism feed bio-enhancer water content
Feeding.By the 5%-10% additions (or substituting the 15% of whole day fine fodder) of ruminant day feeding fine fodder, highest may be added to that
30%.
Beneficial effects of the present invention are：The ruminant biological feedstuff bio-enhancer produced with this method can adjust knurl
Stomach pH value, the optimal acidity under the conditions of making cud be in microecological balance increases the quantity and vigor of protozoon in rumen fluid, strengthens
Absorption and utilization of the animal body to various nutriments in feed.This feed bio-enhancer can improve the physiology machine of body
Can, strengthening the resistance and resistance against diseases of animal body, feeding this product can be such that the milk cow in lactation period output of milk averagely increases after 15 days
Plus 10%, mutton sheep weightening is average to improve 15%, reduces or prevent the use of antibiotic in cultivation.Feature is pure natural, nontoxic nothing
It is side effect, safe and reliable, cost performance high, multi-functional, noresidue, free from environmental pollution.
Brief description of the drawings
Fig. 1 is production technological process of the invention.
Specific embodiment
The preparation method of the ruminant feed bio-enhancer of the present invention of embodiment 1
Experimental group 1 is pressedRuminant feed bio-enhancer of the invention is prepared according to following method：
A) fermentation medium is prepared, by weight：Carbon source is 85%-95%, nitrogen source is 1%-2%, growth promoter is
Mentioned component is crushed, mixed by 1%-3%, balance of water, standby；
B) candida utili, solid fermentation culture, inoculum concentration 10%, 32 are inoculated with step a) gained fermentation mediums
DEG C fermented and cultured 24h；
C) the inoculated plant lactobacillus in step b) gained fermentation mediums, anaerobic solids fermented and cultured samples inspection per 4h
Survey OD600, inoculum concentration 6%, 30 DEG C of anaerobic fermentation culture 20-24h；
D) OD is treated600Into exponential phase, bacillus subtilis, solid are inoculated with step c) gained fermentation mediums
Fermented and cultured, inoculum concentration 4%, 37 DEG C of fermented and cultured 24h.
Control group 1It is prepared as follows ruminant feed bio-enhancer of the invention：
A) fermentation medium is prepared, by weight：Carbon source is 85%-95%, nitrogen source is 1-2%, growth promoter is
Mentioned component is crushed, mixed by 1%-3%, balance of water, standby；
B) bacillus subtilis, solid fermentation culture, inoculum concentration 4%, 37 are inoculated with step a) gained fermentation mediums
DEG C fermented and cultured 24h；
C) the inoculated plant lactobacillus in step b) gained fermentation mediums, anaerobic solids fermented and cultured samples inspection per 4h
Survey OD600, inoculum concentration 6%, 30 DEG C of anaerobic fermentation culture 20-24h；
D) OD is treated600Into exponential phase, candida utili, solid are inoculated with step c) gained fermentation mediums
Fermented and cultured, inoculum concentration 10%, 32 DEG C of fermented and cultured 24h.
Control group 2It is prepared as follows ruminant feed bio-enhancer of the invention：
A) fermentation medium is prepared, by weight：Carbon source is 85%-95%, nitrogen source is 1%-2%, growth promoter is
Mentioned component is crushed, mixed by 1%-3%, balance of water, standby；
B) fermentation medium obtained by step a) is divided into three parts, bacillus subtilis (inoculum concentration 4%) is inoculated with respectively, is planted
After thing lactobacillus (inoculum concentration 6%), candida utili (inoculum concentration 10%), three is well mixed, 33 DEG C of solid fermentation trainings
Support 72h.
The preparation order of the ruminant feed bio-enhancer of the present invention of embodiment 2 is to bacterial content and the shadow of vitamin content
Ring.
The bacterial content and vitamin B complex content of each group ruminant feed bio-enhancer obtained in detection embodiment 1, often
Group is parallel to be carried out 5 times, is averaged, as a result as shown in table 1 below, and is counted.
The viable count and vitamin B complex content of each group ruminant feed bio-enhancer of table 1
By the experimental result, experimental group exists significantly with contrast groups on viable count and vitamin B complex content
Sex differernce, the different inoculation order of bacterial strain can have an immense impact on to the yield of the yield of bacterial strain and vitamin, especially dimension life
Plain B, it is that ruminant is kept fit, and increase is given milk, the key factor of meat productivity, and the present invention particularly sequentially eliminates three kinds
Bacterial strain interfering during the fermentation, increased viable count and vitamin B complex contains volume production.
Influence of the different vaccination amount of embodiment 3 to feed bio-enhancer quality
Method according to experimental example 1 prepares experimental group 2-4, and inoculum concentration is as shown in table 2 below：
The inoculum concentration used in the experimental group 1-4 of table 2
The bacterial content and vitamin B complex content of each group ruminant feed bio-enhancer obtained in detection embodiment 3, often
Group is parallel to be carried out 5 times, is averaged, as a result as shown in table 3 below, and is counted.
The experimental result of the experimental group 1-4 of table 3
By the experimental result, experimental group 1 is deposited with experimental group 2,3 and 4 on viable count and vitamin B complex content
In significant difference.It can thus be appreciated that different inoculum concentrations also can produce influence to the property of product, inoculum concentration reaches doses
Increased dosage amount again, accelerates thalline degree of aging afterwards, causes the viable count in finished product and vitamin B complex content not to rise,
It is possible to decline on the contrary.
Influence of the different fermentations time of embodiment 4 to feed bio-enhancer quality
Method according to experimental example 1 prepares experimental group 2-4, and fermentation time is as shown in table 4 below：
The fermentation time used in the experimental group 1,5-7 of table 4
The bacterial content and vitamin B complex content of each group ruminant feed bio-enhancer obtained in detection embodiment 4, often
Group is parallel to be carried out 5 times, is averaged, as a result as shown in table 5 below, and is counted.
The experimental result of the experimental group 1,5-7 of table 5
By the experimental result, experimental group 1 exists with experimental group 5-7 on viable count and vitamin B complex content
Significant difference.It can thus be appreciated that different fermentation times also can produce influence to the property of product, fermentation time is too short, and fermentation is not
Completely, fermentation time is long, and viable count and vitamin B complex content can be affected.
Influence of the different fermentations temperature of embodiment 5 to feed bio-enhancer quality
Method according to experimental example 1 prepares experimental group 8-10, and fermentation temperature is as shown in table 6 below：
The fermentation time used in the experimental group 1,8-10 of table 6
The bacterial content and vitamin B complex content of each group ruminant feed bio-enhancer obtained in detection embodiment 5, often
Group is parallel to be carried out 5 times, is averaged, as a result as shown in table 7 below, and is counted.
The experimental result of the experimental group 1,8-10 of table 7
By the experimental result, experimental group 1 is deposited with experimental group 8-10 in strain content and vitamin B complex content
In significant difference.It can thus be appreciated that different fermentation temperatures also can produce influence to the property of product, fermentation temperature is too low, fermentation
Not exclusively, fermentation temperature is too high, and viable count and vitamin B complex content can be affected.
Influence of the different fermentations condition of embodiment 6 to the measurement result of feeding animals rumen fluid pH value
1st, experimental technique
Every group is selected healthy cow 5, and before to feed, to 1 hour after feed, 2 hours, 3 hours, 4 hours by stomach tube
It is inserted into cud, directly harvests rumen fluid, and the pH value of rumen fluid is detected with Accurate pH detector.
2nd, experimental result
Experimental result, referring to table 8
The DIFFERENT FEED of table 8 feeds the change of posterior tuberosity gastric juice pH
| Group | Before feed | To after feed 1 | To after feed 2 | To after feed 3 | To after feed 4 |
| PH value | Hour pH value | Hour pH value | Hour pH value | Hour pH value | |
| Experimental group 1 | 6.35 | 6.11 | 6.25 | 6.21 | 6.15 |
| Experimental group 2 | 6.42 | 6.50 | 5.92 | 5.63 | 5.65 |
| Experimental group 5 | 6.56 | 6.21 | 6.01 | 5.85 | 5.84 |
| Experimental group 8 | 6.50 | 6.31 | 5.90 | 5.62 | 5.65 |
| Contrast groups 1 | 6.39 | 6.04 | 5.90 | 6.20 | 5.96 |
| Contrast groups 2 | 6.44 | 6.02 | 5.67 | 5.94 | 6.03 |
By the experimental result, bio-enhancer prepared by different fermentation conditions is for animal rumens liquid pH value meeting
Produce influence, and bio-enhancer of the invention can make being maintained under convenient pH value of rumen fluid, keep ruminant
Cud microecological balance.
Influence of the different fermentations condition of embodiment 7 to Plasmodium falciparum activity in feeding animals rumen fluid
1st, experimental technique
The rumen fluid to 2 hours after feed and 4 hours is obtained with embodiment 3 as sample, with infusorian tally to sample
Protozoon in this is counted, and by calculating the protozoon quantity obtained in rumen fluid.
2nd, experimental result
Experimental result, referring to table 9
The change of rumen fluid Plasmodium falciparum activity after the feeding of the DIFFERENT FEED of table 9
| Group | To 2 hours count values 10 after feed5 | To 4 hours count values 10 after feed5 |
| Experimental group 1 | 7.5 | 7.2 |
| Experimental group 2 | 5.6 | 5.4 |
| Experimental group 5 | 5.1 | 5.0 |
| Experimental group 8 | 5.3 | 5.0 |
| Contrast groups 1 | 5.9 | 5.2 |
| Contrast groups 2 | 6.3 | 5.9 |
By the experimental result, bio-enhancer prepared by different fermentation conditions is lived for animal rumens liquid protozoon
Property produce the influence, and bio-enhancer of the invention can to promote the Plasmodium falciparum activity of animal in rumen fluid, keep animal health shape
State.
Influence of the feed of the present invention of embodiment 8 to the feeding animals output of milk
Every group is selected healthy cow 5, feeds feed of the present invention after 15 days, with control group feed ratio compared with detection lactation period
Milk production of cow, can make the milk cow in lactation period output of milk averagely increase by 10%.
Influence of the feed of the present invention of embodiment 9 to feeding animals meat yield
Every group is selected healthy cow 5, feeds feed of the present invention after 15 days, with control group feed ratio compared with detection mutton sheep body
Weight, mutton sheep weightening is average to improve 15%.
The present invention is not limited to above-mentioned preferred forms, and anyone can show that other are various under enlightenment of the invention
The product of form, however, make any change in its shape or structure, it is every with skill identical or similar to the present application
Art scheme, is within the scope of the present invention.
Claims (7)
1. a kind of preparation method of ruminant feed bio-enhancer, it is comprised the following steps：
A) fermentation medium is prepared, by weight：Carbon source is 85%-95%, nitrogen source is 1%-2%, growth promoter is 1%-
3%, mentioned component is crushed, mixed by balance of water, standby；
B) candida utili, solid fermentation culture are inoculated with step a) gained fermentation mediums；The candida utili
To treat that the inoculum concentration that fermentation medium is counted is 8%-12%, 35-44 DEG C of fermented and cultured 24-28h；
C) the inoculated plant lactobacillus in step b) gained fermentation mediums, anaerobic solids fermented and cultured samples detection per 4h
OD600；The Lactobacillus plantarum is to treat that the inoculum concentration that fermentation medium is counted is 4%-8%, 25-35 DEG C of fermented and cultured 24-28h；
D) OD is treated600Into exponential phase, bacillus subtilis, solid fermentation are inoculated with step c) gained fermentation mediums
Culture；The bacillus subtilis is to treat that the inoculum concentration that fermentation medium is counted is 2%-6%, 28-33 DEG C of fermented and cultured 24-28h.
2. the preparation method of ruminant feed bio-enhancer according to claim 1, it is characterised in that the carbon source
It is maize peel, rice bran, wheat bran, corn flour；The maize peel, rice bran, wheat bran, the part by weight of corn flour are 15-25：
24-34：34-44：3-15.
3. the preparation method of ruminant feed bio-enhancer according to claim 1 and 2, it is characterised in that in hair
Ferment is directly packed after terminating through pellet rotary screen.
4. a kind of ruminant feed bio-enhancer, it is characterised in that it is by such as any one of claim 1-3 methods described
It is prepared.
5. a kind of ruminant feed, it is characterised in that the feed includes bio-enhancer as claimed in claim 4.
6. ruminant feed according to claim 5, it is characterised in that with the gross weight meter of feed, in the feed
Bio-enhancer 1%-54% can be included.
7. the ruminant feed according to claim any one of 5-6, it is characterised in that comprising such as right in the feed
It is required that bio-enhancer 8-13 parts described in 4, corn 34-54 parts, wheat bran 14-25 parts, soya-bean cake 5-24 parts, cottonseed cake 5-24 parts,
Salt 1.2-1.8 parts, soda 1.5-2.3 parts, stone flour 4.8-1.5 parts, bone meal 4.5-1.2 parts, calcium carbonate 4.5-1.2 parts.
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| CN105287651B (en) * | 2015-10-20 | 2019-03-08 | 安徽农业大学 | The application of the preparation method of feeding type probiotics and the feeding type probiotics |
| CN105192250B (en) * | 2015-10-20 | 2019-07-12 | 中国农业科学院农业资源与农业区划研究所 | A kind of feeding type probiotics and its application |
| FR3081090B1 (en) * | 2018-05-15 | 2021-09-10 | Lesaffre & Cie | PROBIOTIC FOR POULTRY |
| CN108740313A (en) * | 2018-06-07 | 2018-11-06 | 沧州新大地生物科技有限公司 | A kind of additive for microbe feedstuff |
| CN111000028A (en) * | 2019-12-30 | 2020-04-14 | 南通励成生物工程有限公司 | Animal feed and preparation method thereof |
| CN113862329B (en) * | 2021-09-09 | 2024-04-09 | 辽宁康普利德生物科技有限公司 | Evaluation method for yeast fermentation performance under simulated digestion condition |
| CN114158650B (en) * | 2021-11-30 | 2024-02-23 | 河南诺尔饲料有限公司 | Fermented full-mixed soft granule feed for young ruminants and preparation method thereof |
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