CN107279462A

CN107279462A - The method of chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder

Info

Publication number: CN107279462A
Application number: CN201710623876.5A
Authority: CN
Inventors: 刘志国; 李铁军; 张建华; 习凤安; 战晓燕; 薛德林
Original assignee: LIAONING VETLAND BIOLOGICAL TECHNOLOGY Co Ltd
Current assignee: LIAONING VETLAND BIOLOGICAL TECHNOLOGY Co Ltd
Priority date: 2017-07-27
Filing date: 2017-07-27
Publication date: 2017-10-24

Abstract

The present invention relates to the method for chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder.Using chrysanthemum meal, dregs of beans, the dense bacterium paste dry powder of arginine, Dried Corn Steep Liquor Powder as raw material, using bacillus subtilis, arginine producing strain, ocean rhodotorula, Lactobacillus plantarum as starting strain, liquid seeds culture and fermented and cultured is respectively adopted.Solid is mixed with liquid proportional uniform, is placed into fermentation bed, and aerobic solids fermented and cultured is carried out through being passed through filtrated air, then drying, crushings, sieving obtains the aquatic products livestock and poultry active peptide powder of chrysanthemum meal fermenting and producing.The present invention has fermentation raw material sources specific, the dense bacterium paste dry powder of chrysanthemum meal, arginine and Dried Corn Steep Liquor Powder is cheap, the product that produces is more after fermentation.Added in feed of this product in aquaculture and livestock and poultry cultivation, improve growth performance, improved the quality of product, breeding environment can also be improved, be a kind of green environmentally friendly type, functional aquatic products livestock and poultry feed addictive.

Description

The method of chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder

Technical field

The present invention relates to technical field of microbial fermentation, more particularly to chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder Method.

Background technology

Chrysanthemum meal also known as chrysanthemum powder are the flowers of marigold of the ground such as northeast, Inner Mongol, Xinjiang plantation after extracting lutein Obtained from byproduct, in green, band threadiness, its property is similar with alfalfa meal, is widely used in the feeds such as poultry, fowl, fish In, the alternative chaff in all feeds proportioning, bran class raw material is good green health type feedstuff.

The main component of chrysanthemum meal：Protein content 12%, grey powder content 8%, moisture 14%.Chrysanthemum meal this category pigment Feed can improve the skin color of animal and the color of eggshell, and contain the various vitamins such as abundant VC, VB.

The characteristics of chrysanthemum meal：1) natural green environmental protection, has no toxic side effect, the livestock raised for meat noresidue that is fed with chrysanthemum powder, without dirt Dye, delicious meat, the yolk of birds, beasts and eggs is bright-coloured, tempting appetite, long shelf-life.2) the medium-height grass property of medicine, clearing away summerheat, with significant clear Heat is dispelled fire, improves the Chinese medicine function of immunity, prevents intestines problem.And can in supplement feed vitamin A deficiency.3) it can replace Wheat bran, wheat-middlings, so as to reduce feeding cost.4) protein can be provided for domestic animals and fowls, and egg yellow can be increased.5) For pig feed, the rush that can be increased weight after fertilizer improves meat and yellowish pink, improves absorptivity.6) it is suitable to aquatic products material, floating force is strong, food calling Function well, can improve the color of the flesh of fish, oppress rubescent, fish scale is yellow by black discoloration, it appears glossy attractive in appearance.In recent years, aquatic products It has been widely used in livestock-raising.

Bacillus subtilis (Bacillus subtilis) is can to produce the aerobic microbiological of gemma.On taxology Belong to prokaryotes, individual cells (0.7~0.8 × 2~3 microns), Gram-positive, no pod membrane, peritrichous can be moved, bud Spore is big (0.6~0.9 × 1.0~1.5 microns), and ellipse arrives column, central or slightly inclined positioned at thalline.Thalline is not swollen after sporulation Greatly, bacterium colony rough surface, it is dirty white or slightly yellow, in Liquid Culture, wrinkle mould is commonly formed.It is mainly distributed on ocean, rivers, lake In pool, activated sludge and soil.Bacillus subtilis has biology aerobic, adjustment flora, suppresses the work(such as germ, biological antagonist Can, so improving animal body immunity, enhancing digestive system enzymatic activity, preventing and curing diseases and playing important work in promoting to grow With.Bacillus subtilis thalline reproduction speed is fast, easily culture, can extracellular proteinase, amylase, lipase and a variety of amino Acid, its gemma strong stress resistance, high temperature resistant, acid and alkali-resistance, anti-extrusion, the features such as application range of products is extensive, and it can be used as mould Formula bacterial strain carries out effective DNA cloning and protein, separation for amino acids on biochip.Domestic and international many scholar's research tables It is bright, bacillus subtilis have in terms of medicines and health protection, aquaculture, livestock culture and ecological, environmental protective higher researching value and Application value.

Arginine producing strain is that Corynebacterium glutamicum (Corynebacterium glutamicum) primarily now applies bacterium Strain is that ZGH-064 is starting strain.Protein is the most basic material for constituting life, protein digesting and assimilating in body Also byAmino acidCome what is completed.Amino acid has the physiology, biochemistry, the immune function of running well for the body that sustains life, and Grow,MetabolismEtc. the normal operation function of vital movement.Arginine belongs to conditionally essential amino acid, arginine master It is the nitrogen balance for maintaining amino acid classes to want function, promotes and maintains normal growth and development, improves and regulation immunologic function, promotes Wound healing, promotes the synthesis of collagen tissue, can repair wound etc..

Ocean rhodotorula (Saccharomyces cerevisiae), is the Rhodotorula in fungi, is nature in ocean A kind of unicellular yeast existed, cellular morphology is rounded or oval, and bacterium colony is red, vegetative propagation, multipole budding, nothingWine Essence fermentationAbility.Protein, glycogen particle, unrighted acid, vitamin, the animal young are rich in cellGrowth hormone, with Based on astaxanthinCarotenoid, thereforeOcean rhodotorulaIt is the animal feed of excellent production of astaxanthin bacterium and great potential Albumen and food additives, while being natural pigment source again, exploitation ocean rhodotorula product is significant.

Ocean rhodotorula resistance is stronger, with good salt tolerance, and more and more extensive weight has been obtained in terms of aquatic products Depending on.Ocean rhodotorula because with comprehensive nutrition, palatability it is good, rich in glucan andManna oligosacchrideIsoreactivity composition, astaxanthin-containing Deng characteristics such as natural pigment and safety non-toxics, aquatic products animal survival rate of seedling can be significantly improved, efficiency of feed utilization, enhancing is improved Animal body immunity and meat Egg Quality, purifying water body, and antibiotic dosage is reduced, it is the excellent additive of ecologic breeding. In the nursery production process such as sea cucumber, shrimp, crab, shellfish extraordinary effect can be reached using concentration ocean rhodotorula.

It is used as eucaryon model organism in modern molecular and cell biology.The cellular morphology of ocean rhodotorula to be spherical or Ovoid, 5~8 μm of diameter.The mode of breeding is gemmation, and its carbon source nitrogen source can utilize glucose, malt using extensive Sugar, sucrose, galactolipin, arabinose, mannitol, butanedioic acid, citric acid, xylose, sorbierite, fructose, ethanol and glycerine；Wine brewing Yeast can be metabolized out various enzyme systems, degraded cellulose and protein with substantial amounts of synthetic yeast albumen.Also enteron aisle simultaneously Prebiotic effect.

Lactobacillus plantarum (Lactobacillus acidophilus) belongs to lactobacillus, Gram-positive bacillus, nose circle Straight-bar bacterium, usually 0.9~1.2vtm × 3.0~8.0 μm are single, in pairs or short chain shape.Flagellum is generally deficient of, but can be moved. Gram-positive, spore of not sprouting.Amphimicrobian, surface colony diameter about 3mm, raised, rounded, surface is smooth, fine and closely woven, color In vain, once in a while in pale yellow or buff.Belong to chemoheterotrophic bacteria, growth needs nutritious culture medium, it is necessary to calcium pantothenate and nicotinic acid, But do not need thiamine, pyridoxal or pyridoxamine, folic acid, vitamin B12.In energy ferment pentoses or gluconate, end-product 85% above is lactic acid.Nitrate is not generally reduced, do not liquefy gelatin, and catalase and oxidizing ferment are all negative.The breasts of DL mono- can be produced Acid, there is 1, the activity of 6~fructose-diphosphate aldolase and hexose monophosphate approach, can be grown in grape hydrochlorate, and produce C02. Ferment the ribose of 1 molecule or the lactic acid of others pentose 1 molecule of generation and the acetic acid of 1 molecule.15 DEG C can grow, generally most suitable life Long temperature is 30~35 DEG C.Anaerobism or amphimicrobian, strain be it is straight or curved shaft-like, it is single, sometimes in pairs or into chain, it is most suitable PH 6.5 or so, belongs to homofermentative lactic bacteria.This bacterium is that the viable count of this bacterium is compared high with the difference of other lactic acid bacterias, energy Substantial amounts of production acid, makes pH value in water is stable not raise, and the acidic materials of its output can degrade heavy metal；Because this bacterium isAnaerobismBacterium (facultative aerobic), energy output is distinctive in reproductive processBacillus acidi lacticiElement, lactobacillin is a kind ofBion Preservative.In the cultivation middle and later periods, due to excrement and residual bait the material increase of animal, the bottom in pond can be sunk to, and is rotted, Grow many germs, generate substantial amounts of ammonia nitrogen andNitrite, bottom is stolen dead phenomenon serious.If plant is used for a long timeLactic acid Bacillus, can just suppress rotting for bottom excrement and residual bait material well, also just reduce ammonia nitrogen andNitriteIncrease, make to support Grow cost reduction.With sour-sweet fragranced, can secrete lactic acid, acetic acid can decline pH value, adjustment gut flora balance, produce Peptide antibiotic, suppresses the propagation of enteron aisle undesirable microorganism, has antagonism to pathogenic microorganisms.Palatability can also be improved, Promote growth, improve food conversion ratio, the usage amount of reduction antibiotic and acidulant reduces cost.Particularly to gastrointestinal function Lack of proper care and take antibiotic and cause the symptoms such as gastrointestinal dysfunction to have special efficacy, the flora in enteron aisle can be made to recover normal rapidly Balance, suppresses the propagation of spoilage organisms, with good nutrition health-care functions, there is certain immunoregulation effect；Have to pathogenic bacteria Inhibitory action；Reduce serum cholesterol content and prevention of cardiovascular disease；Maintain intestinal flora balance；Nutriment is promoted to inhale Receive；Alleviate lactose intolerance；Suppress formation of tumour cell etc..

In conventional research, scientific worker is to bacillus subtilis, ocean rhodotorula and Lactobacillus plantarum and arginine Taxonomy, physiological and biochemical property, fermentation condition and its regulation and control of producing strains, to bacillus subtilis, ocean rhodotorula and The application of Lactobacillus plantarum and arginine producing strain, between bacillus subtilis, ocean rhodotorula and Lactobacillus plantarum three Interactive research and application report etc. it is more.But, to bacillus subtilis, ocean rhodotorula and Lactobacillus plantarum And four kinds of arginine producing strain is few as the research of a community with chrysanthemum meal etc..Bacterium, amino acid and the class of chrysanthemum meal three tool The characteristics of having be：Microbial growth speed is fast, easily culture, thalline and metabolite are nutritious etc..Amino acid can be with Sustain life physiology, biochemistry, the immunity function of body, and grow,MetabolismEtc. the normal operation of vital movement, smart ammonia Acid falls within conditionally essential amino acid, and the main function of arginine is the nitrogen balance for maintaining amino acid kind.Chrysanthemum meal is used as nutrition Material, the bioactive substance such as the protein contained, amino acid, organic calcium, trace element, disease-resistant factor, is conducive to nutrients The conversion of matter absorbs and metabolism, it is possible to reduce the generation of intestines problem, based on above-mentioned advantage：1) the dense bacterium slurry of arginine is utilized Compound amino acid in dry powder, utilizes the high protein in Dried Corn Steep Liquor Powder；2) using the environmentally friendly pigment of natural green in chrysanthemum meal, use The livestock raised for meat noresidue of chrysanthemum powder feeding, pollution-free, delicious meat, the yolk of birds, beasts and eggs are bright-coloured, tempting appetite, long shelf-life.Medium-height grass The property of medicine, clearing away summerheat with significant capable of clearing heat and reducing internal heat, improves the Chinese medicine function of immunity, prevents intestines problem.And feeding can be supplemented The deficiency of vitamin A in material；3) wheat bran, wheat-middlings, so as to reduce feeding cost can be replaced.4) egg can be provided for domestic animals and fowls White matter, and egg yellow can be increased.5) it is used for pig feed, the rush that can be increased weight after fertilizer improves meat and yellowish pink, improves absorptivity. 6) it is suitable to aquatic products material, floating force is strong, and attractant effect is good, can improve the color of the flesh of fish, oppresses rubescent, fish scale is by black discoloration It is yellow, it appears it is glossy it is attractive in appearance wait effect, integrate and fermented, this research is carried out with this, it is creative and innovative grind Study carefully.At present, in aquatic products livestock and poultry cultivation, two large problems are also two hang-ups in face of producers and consumers：1) protein profit It is not high with rate；2) antibiotic usage is excessive.And what the present invention was exactly put forward for solution above-mentioned two problems and problem.

Product function of the present invention is：1) efficiency of feed utilization is improved, allows animal to eat fermented product；2) handled and supported with biological agent The stomach of animal is grown, colony balance is adjusted, harmful bacteria is killed, the usage amount of antibiotic is reduced；3) can be obvious using this product Improvement breeding environment.Simple production process of the present invention, application effect of products is good, can be widely applied to aquatic products, aquatic products livestock and poultry etc. In cultivation industry.

Bacillus subtilis, arginine producing strain, ocean rhodotorula, the thalline of Lactobacillus plantarum and its secretion, smart base Contain abundant protein, amino acid, organic acid, vitamin, unrighted acid and peptide in the dense bacterium paste dry powder of acid and chrysanthemum meal The bioactive substances such as class antibiotic.Applied in the cultivation industries such as aquatic products, aquatic products livestock and poultry, the breeding of cultivated animals can be improved And survival ability, growth, strengthen immunity, premunition and integumentary musculature color and luster can be promoted, promote nutrition digestion to absorb.May be used also With purifying aquaculture environment, purify water, the pollutant such as decomposing organic matter, hydrogen sulfide, amide.It is nontoxic, harmless, secondary make without any With benefiting the nation and the people, be a kind of green microbial ecological preparation, with wide market prospects.

At present, Shenyang Inst. of Applied Ecology, Chinese Academy of Sciences has separated from ocean, purified, having identified many plants of withered grass Bacillus, ocean rhodotorula and Lactobacillus plantarum, by domestication, cultivate, improve obtain many plants of product assay height, activity it is good, The strain of the bacillus subtilis of fast growth, ocean rhodotorula and Lactobacillus plantarum, by our production applications, it is applied Effect is very good, Shenyang, Dalian Area in Liaoning, the area extensive use such as Hebei and Henan, achieves good economy Benefit, product is realized merchandized handling by us.

Bacillus subtilis, ocean rhodotorula and Lactobacillus plantarum and (the micro- life in Chinese Academy of Sciences Beijing of arginine producing strain bacterial strain Thing is bought).The dense bacterium slurry of arginine (from the purchase of Shandong Binzhou Min Qiang biotech inc).Chrysanthemum meal is (from the Xinhe River Morning in Northern Opera week photo-biological Science and Technology Ltd. purchase).

The content of the invention

The technical problem to be solved in the present invention is to provide a kind of chrysanthemum meal fermenting and producing aquatic products aquatic products livestock and poultry active peptide powder Method.

This method using chrysanthemum meal, dregs of beans, the dense bacterium paste dry powder of arginine, Dried Corn Steep Liquor Powder as raw material, with bacillus subtilis, Arginine producing strain, ocean rhodotorula, Lactobacillus plantarum are starting strain, and liquid seeds culture and fermented and cultured is respectively adopted. Solid is mixed with liquid proportional uniform, is placed into fermentation bed, and aerobic solids fermented and cultured is carried out through being passed through filtrated air, then Drying, crushing, sieving obtain the aquatic products livestock and poultry active peptide powder of chrysanthemum meal fermenting and producing.

The present invention has fermentation raw material sources specific, the dense bacterium paste dry powder of chrysanthemum meal, arginine and Dried Corn Steep Liquor Powder price The product that produces is more after cheap, fermentation, nutrition equilibrium, good product quality, using facilitating the features such as effect is good.This product is in aquatic products Add, can prevent and cure diseases in feed in cultivation and livestock and poultry cultivation, improve growth performance, improve the quality of product, can be with Improve breeding environment, be a kind of green environmentally friendly type, functional aquatic products livestock and poultry feed addictive.

The method of chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder, it is dry with chrysanthemum meal, dregs of beans, the dense bacterium slurry of arginine Powder, Dried Corn Steep Liquor Powder are raw material, using bacillus subtilis, arginine producing strain, ocean rhodotorula, Lactobacillus plantarum to go out bacterium germination The weight ratio of various raw materials is in strain, fermentation raw material；50~60 parts of chrysanthemum meal, 10~20 parts of dregs of beans, the dense bacterium paste dry powder of arginine 10~20 parts, 10~20 parts of Dried Corn Steep Liquor Powder；Fermentation raw material and bacillus subtilis, arginine producing strain, ocean rhodotorula, plant The weight ratio of thing lactobacillus seed liquor is：100~120 parts of fermentation raw material:10~20 parts of bacillus subtilis, arginine producing strain 10~20 parts, 10~20 parts of ocean rhodotorula, 10~20 parts of Lactobacillus plantarum；Solid fermentation is carried out in fermentation bed, starting temperature Spend for 20 DEG C~35 DEG C, fermentation temperature is 40 DEG C~60 DEG C, fermentation time is 72 hours~120 hours；Solid fermentation 12 hours Afterwards, ventilation oxygen supply air quantity is 0.1~0.3V/V.min, and ventilation oxygen supply time is, 8：00~17：00, there is yeast during fermentation ends Aroma and lactic taste are produced；Scatter after ventilation, be sent on fluid bed and dry, 60~80 DEG C of EAT, leaving air temp 30 ~40 DEG C, fluidize 8 minutes~12 minutes time, water content 12.0%~15.0% is crushed, crosses the mesh of grit number 80 mesh~100； To chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder.

Comprise the following steps that

(1) preparation of the dense bacterium paste dry powder of arginine：By arginine inoculation to containing glucose, peptone, ammonium sulfate Arginine zymotic fluid is obtained with being fermented in the culture medium of sodium glutamate by 48~64 hours, is added eventually into arginine zymotic fluid Concentration 200~250ppm flocculant Sodium Polyacrylates, ph values are adjusted to 5.5~6.5 with the sulfuric acid of mass fraction 35%~40% Afterwards, it is to react at 40 DEG C~50 DEG C in temperature, the reaction time is 60~80min, then, with 3MPa pressure by reacted essence Propylhomoserin zymotic fluid is pressed into filter press, and time of filter pressing is 40~60 minutes, obtains the arginine that water content is 35%~40% Dense bacterium solution wet-milling, then dried, obtain the dense bacterium paste dry powder of arginine that water content is 10%~15%；

(2) prepared by fermentation raw material：The dense bacterium of 50~60 parts of chrysanthemum meal, arginine in fermentation raw material is starched dry in required ratio 10~20 parts of powder, 10~20 parts of dregs of beans, 10~20 parts of Dried Corn Steep Liquor Powder；

(3) preparation of zymocyte liquid：10~20 parts of bacillus subtilis seed liquor, 10~20 parts of arginine producing strain, sea 10~20 parts of fuchsin saccharomycete seed liquor, 10~20 parts of of Lactobacillus plantarum seed liquor

Fermentation raw material and bacillus subtilis seed liquor, arginine producing strain, ocean rhodotorula bacterium seed liquor, the newborn bar of plant The weight ratio of bacterium seed liquor mixed liquor is 100~120 parts of fermentation raw material, 40~60 parts of mixed liquor.

The method of chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder, it is characterised in that：PH value is during fermentation ends 7.0, total acid is 200 ° of T；

Product crude protein 50% during fermentation ends, wherein, containing short-chain peptide more than 20.8%, (short chain peptide molecular weight is 2000 Below dalton), lysine 2.16%, methionine 1.16%, threonine 1.26%, tryptophan 0.63%, arginine 3.6%；

Every milliliter contains viable count >=100 hundred million in bacillus subtilis and arginine producing strain seed liquor.Ocean rhodotorula Every milliliter contains viable count >=50 hundred million in bacterium and Lactobacillus plantarum seed liquor；

The mass water content of each material in fermentation raw material is chrysanthemum meal 10%, dregs of beans 10%, the dense bacterium paste dry powder of arginine 10%th, Dried Corn Steep Liquor Powder 10%, the gross mass water content of the fermentation raw material are 10%.

The weight ratio of various raw materials is in fermentation raw material；Fermentation raw material and bacillus subtilis, arginine producing strain, ocean Rhodotorula, the weight ratio of Lactobacillus plantarum seed liquor are：100~120 parts of fermentation raw material:10~20 parts of bacillus subtilis, essence 10~20 parts of propylhomoserin producing strains, 10~20 parts of ocean rhodotorula, 10~20 parts of Lactobacillus plantarum；Solid fermentation is enterprising in fermentation bed OK, initial temperature is 20 DEG C~35 DEG C, and fermentation temperature is 40 DEG C~60 DEG C, and fermentation time is 72 hours~120 hours；Solid is sent out After ferment 12 hours, ventilation oxygen supply air quantity is 0.1~0.3V/V.min, and ventilation oxygen supply time is, 8：00~17：00, fermentation ends When there is yeast aroma and lactic taste to produce；Scatter after ventilation, be sent on fluid bed and dry, 60~80 DEG C of EAT goes out 30~40 DEG C of air temperature, 8 minutes~12 minutes time of fluidisation, water content 12.0%~15.0%, crushing, the excessively mesh of grit number 80~ 100 mesh；Obtain chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder.

(4) aerobic and anaerobic solids fermentation stage：

In required ratio by bacillus subtilis seed liquor, bacillus licheniformis seed liquor, fermented glutinous rice yeast bacterium seed liquor, thermophilic Lactobacillus lactis seed liquor is mixed, and is inoculated into prepared fermentation raw material, carries out solid fermentation；

1) aerobic solids fermentation stage：24 hours aerobic fermentations, are not translated into, stuffy, and temperature is from room temperature, slow rise, Temperature control is in 25 DEG C~35 DEG C, mass water content 40%；

2) anaerobic solids fermentation stage：24 hours aerobic fermentations：Do not translate into, it is stuffy, after temperature rise, maintain balance to rise Height, temperature control is at 35 DEG C~45 DEG C, and mass water content 35% starts the product in decomposing and fermenting material；

3) lead to sterile wind and become the gemma stage:When temperature is raised to 45 DEG C~50 DEG C of peak, start to translate into fermentation material, temperature Begin to decline, mass water content 25%, water activity is very low, fermentation reaches terminal, continuation is aerated to fermentation bed top fermentation material, Gemma occurrence rate reaches 80%, stops ventilation, after placing 10 hours, starts drying, by crushing, sieving, obtaining chrysanthemum meal hair The aquatic products livestock and poultry active peptide powder of ferment production.

(5) preparation of bacillus subtilis seed liquor：

1) single bacterium colony is separated：Activated bacillus subtilis slant strains are being filled into the glucose beef extract that newly configures It is coated with the culture dish of peptone bacillus subtilis bacterium culture medium, 30 DEG C of cultures, obtain bacillus subtilis in constant incubator Bacterium single bacterium colony seed；

2) solid seed culture：Bacillus subtilis single bacterium colony seed is filling glucose beef extract-peptone withered grass gemma It is inoculated with the flat bottle of 200ml eggplant shapes of baccilus medium, 30 DEG C of cultures, obtain bacillus subtilis solid kind in constant incubator Son；

3) liquid seeds culture：Bacillus subtilis solid seed is connect by mass fraction for 10%~20% inoculum concentration Plant into seed tank culture base, through culture, obtain bacillus subtilis seed liquor；The seed tank culture base is with percentage by weight It is calculated as：Glucose 0.5~1.5%, Dried Corn Steep Liquor Powder 2.0~4.0%, ammonium sulfate 0~0.2%, dipotassium hydrogen phosphate 0~0.2%, Surplus is water；Seeding tank sterilising conditions, 0.08~0.10MPa, 115~121 DEG C, 18~20min；Secondary liquid seed culture bar Part, tank presses 0.01~0.10MPa, air quantity 100:80~100:120vvm, 100~200rpm of rotating speed, temperature DEG C 28 DEG C~32 DEG C, when Between 48~64h；The cfu/ml of viable count containing bacillus subtilis in bacillus subtilis seed liquor >=10,000,000,000；

(6) preparation of arginine producing strain seed liquor：

1) single bacterium colony is separated；2) solid seed culture：3) liquid seeds culture；Preparation process is with above-mentioned (5).

Difference is to cultivate and constitutes different.

Arginine producing strain seed tank culture base：It is by weight percentage：Glucose 1.5%~2.0%, glutamic acid 1.5%~2.0%, Dried Corn Steep Liquor Powder 3.0%~3.5%, ammonium sulfate 0.1%~0.2%, magnesium sulfate 0.1%~0.2%, phosphorus Sour hydrogen dipotassium 0.1%~0.2%, surplus is water, pH6.8~7.0；Sterilising conditions：115~121 DEG C of temperature, pressure 0.08~ 0.10MPa, 18~20min of time；Condition of culture：Inoculum concentration 10~20%；Tank presses 0.01~0.10MPa, air quantity 100:100~ 100:200vvm, 100~200rpm of rotating speed, 30 DEG C~32 DEG C of temperature；48~64h of time.

(7) preparation of ocean rhodotorula seed liquor：

1) single bacterium colony is separated：Activated ocean rhodotorula bacterium slant strains are being filled into the glucose proteins peptone that newly configures It is coated with the culture dish of yeast extract medium, 30 DEG C of cultures, obtain ocean rhodotorula single bacterium colony seed in constant incubator；

2) solid seed culture：Ocean rhodotorula single bacterium colony seed is filling glucose proteins peptone yeast extract medium It is inoculated with the flat bottle of 200ml eggplant shapes, 30 DEG C of cultures, obtain ocean rhodotorula bacterium solid seed in constant incubator；

3) liquid seeds culture：Ocean rhodotorula solid seed is inoculated with by mass fraction for 10%~20% inoculum concentration Into seed tank culture base, through culture, saccharomycete seed liquor is obtained；The seed tank culture base is by weight percentage：Portugal Grape sugar 2.0~4.0%, Dried Corn Steep Liquor Powder 2.0~4.0%, ammonium sulfate 0~0.2%, dipotassium hydrogen phosphate 0~0.2%, surplus is Water；Secondary seed tank sterilising conditions：Tank presses 0.08~0.10MPa, 115~121 DEG C of temperature, 18~20min of time；Secondary liquid Seed culture condition：Tank presses 0.01~0.10MPa, air quantity 100:100~100:150vvm, 180~220rpm of rotating speed, temperature 28 DEG C~36 DEG C, 48~64h of time；The cfu/ml of viable count containing ocean rhodotorula in ocean rhodotorula seed liquor >=5,000,000,000.

(8) preparation of Lactobacillus plantarum seed liquor：

1) single bacterium colony is separated：Activated Lactobacillus plantarum slant strains are being filled into the glucose proteins peptone ox that newly configures It is coated with the culture dish of meat extract yeast extract medium, 30 DEG C of cultures, obtain Lactobacillus plantarum single bacterium planting in constant incubator Son；

2) solid seed culture：Lactobacillus plantarum single bacterium colony seed is filling glucose proteins peptone beef extract yeast extract culture It is inoculated with the flat bottle of 200ml eggplant shapes of base, 30 DEG C of cultures, obtain Lactobacillus plantarum solid seed in constant incubator；

3) liquid seeds culture：By Lactobacillus plantarum one-level solid seed by the inoculum concentration that mass fraction is 10%~20% It is inoculated into seed tank culture base, through culture, obtains Lactobacillus plantarum seed liquor；The seed tank culture base is with percentage by weight It is calculated as：Glucose 2.0~4.0%, Dried Corn Steep Liquor Powder 2.0~4.0%, ammonium sulfate 0~0.2%, dipotassium hydrogen phosphate 0~0.2%, Surplus is water；Secondary seed tank sterilising conditions：Tank presses 0.08~0.10MPa, 115~121 DEG C of temperature, 18~20min of time；Two Level liquid seeds condition of culture：Tank presses 0.01~0.10MPa, air quantity 100:100~100:150vvm(100:200vvm), rotating speed 180~220rpm, temperature 28 DEG C~36 DEG C (32 DEG C), 48~64h of time；Lived in Lactobacillus plantarum seed liquor containing Lactobacillus plantarum The cfu/ml of bacterium number >=5,000,000,000.

It is preferred that step (1) in, by arginine producing strain strain be inoculated into containing glucose, peptone, ammonium sulfate and paddy ammonia Arginine zymotic fluid is obtained by fermentation in 64 hours in the culture medium of sour sodium, final concentration 250ppm is added into arginine zymotic fluid Flocculant Sodium Polyacrylate, ph values are adjusted to after 6.5 with the sulfuric acid of mass fraction 40%, are to react at 50 DEG C in temperature, are reacted Time is 80min, then, reacted arginine zymotic fluid is pressed into filter press with 3MPa pressure, time of filter pressing is 60 minutes, obtain the dense bacterium solution wet-milling of arginine that water content is 35%, the dense bacterium paste dry powder of 10% arginine after drying.

It is preferred that step (2) in, the dense bacterium paste dry powder of chrysanthemum meal, dregs of beans, arginine in fermentation raw material, Dried Corn Steep Liquor Powder are filled Divide mixing；The composition of fermentation raw material is 50 parts of chrysanthemum meal, 10 parts of dregs of beans, 20 parts of the dense bacterium paste dry powder of arginine, Dried Corn Steep Liquor Powder 20 Part；

It is preferred that step (3) in, bacillus subtilis seed liquor, arginine producing strain seed liquor, ocean rhodotorula seed Liquid, the percentage by weight of Lactobacillus plantarum seed liquor are 10 parts of bacillus subtilis seed liquor, arginine producing strain seed liquor 10 Part, 10 parts of ocean rhodotorula seed liquor, 10 parts of Lactobacillus plantarum seed liquor,.

Fermentation raw material and seed liquor mixed liquor weight ratio：100 parts of fermentation raw material；40 parts of seed mixed liquor.

It is preferred that step (4) in, the fermentation initial mass water content 40% of fermentation material, initial ph value is 6, fermentation starting temperature Spend for 25 DEG C, process control temp is 35 DEG C~45 DEG C, fermentation time is 100 hours, ph values are 6, and fermentation reaches terminal；Continue Fermentation bed top fermentation material is aerated, reduction mass water content is then communicated on fluid bed, under the conditions of 60 DEG C to 20% Drying, drying is arrived after mass water content≤10%, is crushed under aseptic conditions, crosses 100 mesh sieves, obtains chrysanthemum meal hair Ferment produces aquatic products livestock and poultry active peptide powder.

It is preferred that step (5) in, the preparation of bacillus subtilis seed liquor：

3) liquid seeds culture：Bacillus subtilis solid seed is inoculated into kind by mass fraction for 10% inoculum concentration In sub- tank culture medium, through culture, bacillus subtilis seed liquor is obtained；The seed tank culture base is by weight percentage： Glucose 0.5%, Dried Corn Steep Liquor Powder 4.0%, sulfuric acid 0.2%, dipotassium hydrogen phosphate 0.2%, surplus is water；Seeding tank sterilizing bar Part, 0.08MPa, 115 DEG C, 20min；Condition of culture, tank pressure 0.05MPa, air quantity 100:120vvm, rotating speed 100rpm, temperature 32 DEG C, time 48h；The cfu/ml of viable count containing bacillus subtilis in bacillus subtilis seed liquor >=10,000,000,000；

It is preferred that step (6) in, the preparation of arginine producing strain seed liquor seed liquor：

Difference is to cultivate and constitutes different.

Arginine producing strain seed tank culture base：It is by weight percentage：Glucose 2.0%, glutamic acid 2.0%, jade Rice ＆ peanut milk dry powder 3.0%, ammonium sulfate 0.1%, magnesium sulfate 0.1%, dipotassium hydrogen phosphate 0.1%, surplus are water, pH6.8；Sterilize bar Part：115 DEG C of temperature, pressure 0.08MPa, time 18min；Condition of culture：Inoculum concentration 10%；Tank pressure 0.05MPa, air quantity 100: 200vvm, rotating speed 200rpm, 32 DEG C of temperature；Time 48h.

It is preferred that step (7) in, the preparation of ocean rhodotorula seed liquor：

2) solid seed culture：Ocean rhodotorula single bacterium colony seed is filling glucose proteins peptone yeast extract medium It is inoculated with the flat bottle of 200ml eggplant shapes, 30 DEG C of cultures, obtain ocean rhodotorula solid seed in constant incubator；

3) liquid seeds culture：Ocean rhodotorula solid seed is inoculated into seed by mass fraction for 10% inoculum concentration In tank culture medium, through culture, ocean rhodotorula seed liquor is obtained；The seed tank culture base is by weight percentage：Grape Sugar 2.0%, Dried Corn Steep Liquor Powder 2.0%, ammonium sulfate 0.2%, dipotassium hydrogen phosphate 0.2%, surplus is water；Seeding tank sterilising conditions： Tank pressure 0.08MPa, 115 DEG C of temperature, time 20min；Seed culture condition：Tank pressure 0.05MPa, air quantity 100:150vvm, rotating speed 180rpm, 28 DEG C of temperature, time 48h；The cfu/ml of viable count containing ocean rhodotorula in ocean rhodotorula seed liquor >=5,000,000,000.

It is preferred that step (8) in, the preparation of Lactobacillus plantarum seed liquor：

1) single bacterium colony is separated：Activated Lactobacillus plantarum lactobacillus slant strains are being filled into the glucose egg that newly configures It is coated with the culture dish of white peptone beef extract yeast extract medium, 30 DEG C of cultures, obtain Lactobacillus plantarum list in constant incubator Bacterium colony seed；

3) liquid seeds culture：Bacterium Lactobacillus plantarum one-level solid seed is inoculated with by mass fraction for 10% inoculum concentration Into seed tank culture base, through culture, Lactobacillus plantarum seed liquor is obtained；The seed tank culture base is by weight percentage For：Glucose 2.0%, Dried Corn Steep Liquor Powder 2.0%, ammonium sulfate 0.2%, dipotassium hydrogen phosphate 0.2%, surplus is water；Secondary seed Tank sterilising conditions：Tank pressure 0.08MPa, 115 DEG C of temperature, time 20min；Condition of culture：Tank pressure 0.05MPa, air quantity 100: 100vvm, rotating speed 180rpm, 28 DEG C of temperature, time 48h；The cfu/ of number containing live lactobacillus acidophilus in saccharomycete seed liquor >=5,000,000,000 ml。

It is using the beneficial effect produced by above-mentioned technical proposal：

(1) fermentation raw material chrysanthemum meal of the invention and starting strain ocean rhodotorula are all blue or green containing substantial amounts of vitamin and shrimp Element, and some organic pigments, the vitamin for supplementing animal body, anti allergic reaction, to the yellowish pink and egg of aquatic products and pork Riboflavin etc. have good improvement.

(2) present invention utilizes smart ammonia using the dense bacterium paste dry powder of chrysanthemum meal arginine and Dried Corn Steep Liquor Powder as main fermentation raw material The available nitrogen and rapid available phosphorus of the dense bacterium paste dry powder of acid and Dried Corn Steep Liquor Powder, it is red as bacillus subtilis, Amino Acid-producing Bacteria, ocean Yeast and the nitrogen source of Lactobacillus plantarum fermentation, rapid breeding amplification are transformed into microorganism animal protein, and by the dense bacterium of arginine Nonprotein nitrogen in paste dry powder and Dried Corn Steep Liquor Powder, fermentation process is absorbed, and is degraded to the absorbable amino acid utilized And short-chain peptide, so that its protein utilization rate is greatly improved.Using humic acid as main fermentation raw material, it is possible to use it contains abundant The bioactive substance, disease-resistant solution such as protein, amino acid, organic acid, vitamin, unrighted acid and peptide antibiotics Poison, improves breeding and the survival ability of cultivated animals, can promote growth, strengthen immunity, premunition and integumentary musculature color and luster, Nutrition digestion is promoted to absorb, purifying aquaculture environment purifies water.

(3) there is the present invention fermentation raw material source to be specific, and the dense bacterium paste dry powder of arginine and Dried Corn Steep Liquor Powder are cheap It is easy to get, simple production process, with short production cycle, the features such as tunning is more, good product quality, application effect are good.This product exists Add, can prevent and cure diseases in feed in precious marine product, aquatic products livestock and poultry cultivation, improve survival rate, improve immunity, improve growth Speed, improves quality；Because feed of the present invention is activated feed, breeding environment can be improved, purified water, be a kind of green Efficiently, the aquatic products livestock and poultry active peptide powder of the chrysanthemum meal fermenting and producing of nontoxic, harmless high-quality, with wide market prospects.

(4) present invention can be metabolized various bioactivators such as, several amino acids are small in fermentation process Peptide, is combined the materials such as enzyme, and the high molecular weight protein in raw material is progressively decomposed into simple material, then by complicated physical chemistry With biochemical reaction, formed with unique fermentation material local flavor.Protein feed utilization rate can be greatly improved, and has work Bacterium produces various metabolites in breeding, so, the stomach of cultivated animals can be handled, colony balance is adjusted, harmful bacteria is killed, Reduce the usage amount of antibiotic.

Embodiment

(bacillus subtilis, saccharomyces cerevisiae, lactobacillus acidophilus and amino acid preparation strain are from Chinese Academy of Sciences Beijing microorganism Bought.The dense bacterium slurry of arginine is bought from Shandong Binzhou Min Qiang biotech inc.Chrysanthemum meal is all from Xinhe River Northern Opera Morning photo-biological Science and Technology Ltd. purchase.)

Embodiment 1

The preparation of the dense bacterium solution dry powder of arginine：

Arginine bacterium producing bacterial strain is inoculated into the culture medium containing glucose, peptone, ammonium sulfate and sodium glutamate Arginine zymotic fluid is obtained by fermentation in 64 hours.16 tons of arginine zymotic fluids are driven into 20 tons of containers, stood.To smart ammonia Final concentration 250ppm flocculant Sodium Polyacrylates are added in acid fermentation liquid, ph values are adjusted to 6.5 with the sulfuric acid of mass fraction 40% Afterwards, it is to react at 50 DEG C in temperature, the reaction time is 80min, then, with 3MPa pressure by reacted arginine zymotic fluid It is pressed into filter press, time of filter pressing is 60 minutes, obtains the dense bacterium solution wet-milling of arginine that water content is 35%, be dried into 10% The dense bacterium paste dry powder of arginine.

(2) preparation of bacillus subtilis seed liquor：

3) liquid seeds culture：Bacillus subtilis solid seed is inoculated into kind by mass fraction for 10% inoculum concentration In sub- tank culture medium, through culture, bacillus subtilis seed liquor is obtained；The seed tank culture base is by weight percentage： Glucose 0.5%, Dried Corn Steep Liquor Powder 4.0%, sulfuric acid 0.2%, dipotassium hydrogen phosphate 0.2%, surplus is water；Seeding tank sterilizing bar Part, 0.08MPa, 115 DEG C, 20min；Condition of culture, tank pressure 0.05MPa, air quantity 100:120vvm, rotating speed 100rpm, temperature 32 DEG C, time 48h；

4) detection of bacterium number and its evaluation of fermentation ends：Determined using colony counting method in bacillus subtilis seed liquor The cfu/ml of viable count containing bacillus subtilis >=10,000,000,000.Test under microscope bacillus subtilis seed liquor, thalline motion is lived Sprinkle, healthy and strong, neatly, no miscellaneous bacteria, free from extraneous odour, the anomaly such as pollution-free, fermentation ends can proportionally be mixed with other seed liquors Conjunction is used.

(3) preparation of arginine producing strain seed liquor seed liquor：

1) single bacterium colony is separated；2) solid seed culture：3) liquid seeds culture；4) detection of bacterium number and its fermentation ends Evaluate：Preparation process is with above-mentioned (5).

Difference is to cultivate and constitutes different.

(4) preparation of fermented glutinous rice yeast bacterium seed liquor：

1) single bacterium colony is separated：Activated fermented glutinous rice yeast bacterium slant strains are being filled into the glucose proteins peptone ferment that newly configures It is coated with the culture dish of female cream culture medium, 30 DEG C of cultures, obtain fermented glutinous rice yeast single bacterium colony seed in constant incubator；

2) solid seed culture：Fermented glutinous rice yeast single bacterium colony seed is filling glucose proteins peptone yeast extract medium It is inoculated with the flat bottle of 200ml eggplant shapes, 30 DEG C of cultures, obtain saccharomycete solid seed in constant incubator；

3) liquid seeds culture：Fermented glutinous rice yeast solid seed is inoculated into seeding tank by mass fraction for 10% inoculum concentration In culture medium, through culture, saccharomycete seed liquor is obtained；The seed tank culture base is by weight percentage：Glucose 2.0%th, Dried Corn Steep Liquor Powder 2.0%, ammonium sulfate 0.2%, dipotassium hydrogen phosphate 0.2%, surplus is water；Seeding tank sterilising conditions：Tank Press 0.08MPa, 115 DEG C of temperature, time 20min；Seed culture condition：Tank pressure 0.05MPa, air quantity 100:150vvm, rotating speed 180rpm, 28 DEG C of temperature, time 48h；The cfu/ml of viable count containing fermented glutinous rice yeast in saccharomycete seed liquor >=5,000,000,000.

4) detection of bacterium number and its evaluation of fermentation ends：Determined and contained in ocean rhodotorula seed liquor using colony counting method The cfu/ml of ocean rhodotorula viable count >=5,000,000,000.Thalline motion is active, healthy and strong, neatly, no miscellaneous bacteria, free from extraneous odour, pollution-free etc. different Chang Xianxiang, fermentation ends can be proportionally used in mixed way with other seed liquors.

(5) preparation of Lactobacillus plantarum seed liquor：

2) solid seed culture：Lactobacillus acidophilus single bacterium colony seed is filling glucose proteins peptone beef extract yeast extract culture It is inoculated with the flat bottle of 200ml eggplant shapes of base, 30 DEG C of cultures, obtain Lactobacillus plantarum solid seed in constant incubator；

3) liquid seeds culture：Thermophilic Lactobacillus plantarum one-level solid seed is inoculated with by mass fraction for 10% inoculum concentration Into seed tank culture base, through culture, lactobacillus acidophilus seed liquor is obtained；The seed tank culture base is by weight percentage For：Glucose 2.0%, Dried Corn Steep Liquor Powder 2.0%, ammonium sulfate 0.2%, dipotassium hydrogen phosphate 0.2%, surplus is water；Secondary seed Tank sterilising conditions：Tank pressure 0.08MPa, 115 DEG C of temperature, time 20min；Condition of culture：Tank pressure 0.05MPa, air quantity 100: 100vvm, rotating speed 180rpm, 28 DEG C of temperature, time 48h；

4) detection of bacterium number and its evaluation of fermentation ends：Cellular morphology is that under the conditions of pH value is below 5.0, thalline is presented Rod-short, thalline is sturdy, two ends blunt circle, and under the conditions of pH value 6.2, thalline attenuates, shaft-like longer.Culture dish is checked：Flat board is trained It is in water white transparency to support the Lactobacillus plantarum bacterium colony on base, is determined using colony counting method in Lactobacillus plantarum seed liquor containing acidophilus breast The cfu/ml of bacillus viable count >=5,000,000,000.When culture is ripe, below PH=5, with sour-sweet fragranced, thalline motion is active, healthy and strong, Neatly, no miscellaneous bacteria, free from extraneous odour, the anomaly such as pollution-free, fermentation ends can be proportionally used in mixed way with other seed liquors.

Chrysanthemum meal water content is that 10%, dregs of beans water content is the dense bacterium paste dry powder water content of 10%, arginine in fermentation raw material It is 10% for 10%, Dried Corn Steep Liquor Powder water content.Total moisture content 10.0% in fermentation raw material.

(6) prepared by fermentation raw material：The chrysanthemum meal in fermentation raw material, dregs of beans, arginine underflow bacterium solution are done in required ratio Powder, Dried Corn Steep Liquor Powder are sufficiently mixed；10 parts of 50 parts of chrysanthemum meal, dregs of beans, 20 parts of arginine underflow bacterium dry powder, jade in fermentation raw material 20 parts of Rice ＆ peanut milk dry powder；

Bacillus subtilis seed liquor, arginine producing strain seed liquor, ocean rhodotorula bacterium seed liquor, Lactobacillus plantarum kind The percentage by weight of sub- liquid is 10 parts of bacillus subtilis seed liquor, 10 parts of arginine producing strain seed liquor, ocean rhodotorula bacterium 10 parts of seed liquor, 10 parts of Lactobacillus plantarum seed liquor；

Fermentation raw material and bacillus subtilis seed liquor, arginine producing strain seed liquor, ocean rhodotorula bacterium seed liquor, plant The mixed liquor weight ratio of thing lactobacillus seed liquor is 100 parts of fermentation raw material, bacillus subtilis seed liquor, arginine producing strain kind Sub- liquid, ocean rhodotorula bacterium seed liquor, the mixed liquor of Lactobacillus plantarum seed liquor are 40 parts.

(7) aerobic and anaerobic solids fermentation stage：

The fermentation initial mass water content 40% of fermentation material, initial ph value is 6, and fermentation initial temperature is 25 DEG C, excessively program control Temperature processed is 40 DEG C~45 DEG C, and fermentation time is 100 hours, and ph values are 6, and fermentation reaches terminal；Continue to fermentation bed top fermentation Material is aerated, and reduction mass water content is then communicated on fluid bed to 20%, is dried under the conditions of 60 DEG C, and matter is arrived in drying Measure after water content≤15%, crushed under aseptic conditions, cross 100 mesh sieves, obtain the aquatic products of chrysanthemum meal fermenting and producing Livestock and poultry active peptide powder

Embodiment 2

The chrysanthemum meal fermenting and producing aquatic products livestock and poultry that embodiment 1 is obtained are being answered with active peptide powder in culture of Penaeus vannamei With experiment, plan design is shown in Table 1：

Table 1, chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder plan design

Project	Experiment 1	Experiment 2	Experiment 3	Control
					Consumption	B+3%	B+4%	B+5%	Bait (B)

With active peptide powder, the application test in culture of Penaeus vannamei the results are shown in Table 2 to chrysanthemum meal fermenting and producing aquatic products livestock and poultry：

The chrysanthemum meal fermenting and producing aquatic products livestock and poultry of table 2. are with active peptide powder to Penaeus Vannmei gain in weight, survival rate and feed report Reward rate influences (30 days)

* 26 DEG C of water temperature；* culturing pool bulk area：Length × width × height=5m × 4m × 1.5m=30m³；Pool side is fillet.

* P values are examined to be less than 0.01 difference with control group t by mathematical statistics test group extremely notable.

* control group is with normal bait (B)

It can be seen from Table 2 that, under normal condition and equal managerial skills, by the nursing of 30 days, test group Price of deed rate (FCR) 0.88, control group price of deed rate (FCR) 1.03, test group drops compared with control group price of deed rate (FCR) It is low by 0.15；Test group survival rate 96.67%, control group survival rate 94.67%, test group is improved compared with control group survival rate 1.02%；Test group total augment weight 115.15g/30 days, control group total augment weight 104.89g/30 days, test group is compared with control group rate of body weight gain Improve 9.78%.Result of the test shows：In bait addition 5% flower dregs of rice fermenting and producing aquatic products livestock and poultry with active peptide powder to South America The impact effect of white shrimp weightening dose rate, survival rate and price of deed rate is obvious.

Embodiment 3

The chrysanthemum meal fermenting and producing aquatic products livestock and poultry that embodiment 1 is obtained apply examination with active peptide powder in Grouper cultivating Test, plan design is shown in Table 3：

Table 3, chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder plan design

With active peptide powder, the application test in Grouper cultivating the results are shown in Table 4 to chrysanthemum meal fermenting and producing aquatic products livestock and poultry：

The chrysanthemum meal fermenting and producing aquatic products livestock and poultry of table 4. are with active peptide powder to grouper gain in weight, survival rate and price of deed rate Influence (30 days)

* control group is with normal bait (B)

It can be seen from Table 4 that, under normal condition and equal managerial skills, by the nursing of 30 days, test group Price of deed rate (FCR) 0.798, control group price of deed rate (FCR) 1.062, test group is compared with control group price of deed rate (FCR) Reduction by 0.264；Test group survival rate 96.0%, control group survival rate 93.0%, test group is improved compared with control group survival rate 1.03%；Test group total augment weight 2175.4g/30 days, control group total augment weight 2168.3g/30 days, test group is compared with control group rate of body weight gain Improve 9.28%.Result of the test shows：In bait addition 5% chrysanthemum meal fermenting and producing aquatic products livestock and poultry with active peptide powder to stone The impact effect of spot fish weightening dose rate, survival rate and price of deed rate is obvious.

Embodiment 4

The chrysanthemum meal fermenting and producing aquatic products livestock and poultry that embodiment 1 is obtained are with active peptide powder product in aquatic products livestock and poultry cultivation The big porker stage carry out application test, by chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder, in big porker stage daily ration according to 2%~5% is added, and by the feeding trial of 35 days, chrysanthemum meal fermenting and producing aquatic products livestock and poultry were given birth to active peptide powder to big porker The influence of long performance the results are shown in Table 5.

Influence of the chrysanthemum meal fermenting and producing aquatic products livestock and poultry of table 5. with active peptide powder to growing and fattening pigs growth period performance

It can be seen from Table 5 that：Chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder, in big porker stage daily ration according to 0.2%~0.5% is added, and by the feeding trial of 35 days, as a result shows：1.03 kilograms of 1 average daily gain of test group, examination Test and organize 1.05 kilograms of 2 average daily gain, 0.95 kilogram of control group average daily gain, test group 1 is carried with control group average daily gain High by 8.13%, test group 2 improves 10.54% with control group average daily gain.The feed-weight ratio of test group 1 is 3.13：1, test group 2 Feed-weight ratio is 3.1：1, control group feed-weight ratio is 3.27：1, test group 1 reduces by 4.24%, test group with control group feed-weight ratio 2 reduce by 5.16% with control group feed-weight ratio.There is no diarrhea of pigs in process of the test, and excrement is soft, and color is light yellow, smelly Taste mitigates, and breeding environment is obviously improved.

Embodiment 5

The chrysanthemum meal fermenting and producing aquatic products livestock and poultry that embodiment 1 is obtained are with active peptide powder to hy-line brown hen laying rate and material egg The influence experiment of ratio, is added, addition is 2%, can improve its laying rate in the blue brown laying hen drinking water in sea of 28 week old, Its feedstuff-egg ratio is reduced, compared with control group, laying rate improves 1.1, and feedstuff-egg ratio declines 0.9.Result of the test is shown in Table 6：

The influence of table 6, chrysanthemum meal fermenting and producing aquatic products livestock and poultry with active peptide powder to hy-line brown hen laying rate and feedstuff-egg ratio

Note：Experimental animal number every group 500；28 week old begin to use；Experimental period is 30 days；

The present invention has fermentation raw material sources specific, and chrysanthemum meal and arginine underflow bacterium solution dry powder are cheap, ferment The product produced afterwards is more, nutrition equilibrium, good product quality, using facilitating the features such as effect is good.This product is in aquaculture and poultry Add, can prevent and cure diseases in feed in fowl cultivation, improve growth performance, improve the quality of product, cultivation can also be improved Environment, is a kind of green environmentally friendly, functional aquatic products livestock and poultry feed addictive.

Claims

1. the method for chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder, it is characterised in that：With chrysanthemum meal, dregs of beans, arginine Dense bacterium paste dry powder, Dried Corn Steep Liquor Powder are raw material, with bacillus subtilis, arginine producing strain, ocean rhodotorula, Lactobacillus plantarum For starting strain, the weight ratio of various raw materials is in fermentation raw material；50~60 parts of chrysanthemum meal, 10~20 parts of dregs of beans, arginine are dense 10~20 parts of bacterium paste dry powder, 10~20 parts of Dried Corn Steep Liquor Powder；Fermentation raw material and bacillus subtilis, arginine producing strain, ocean Rhodotorula, the weight ratio of Lactobacillus plantarum seed liquor are：100~120 parts of fermentation raw material:10~20 parts of bacillus subtilis, essence 10~20 parts of propylhomoserin producing strains, 10~20 parts of ocean rhodotorula, 10~20 parts of Lactobacillus plantarum；Solid fermentation is enterprising in fermentation bed OK, initial temperature is 20 DEG C~35 DEG C, and fermentation temperature is 40 DEG C~60 DEG C, and fermentation time is 72 hours~120 hours；Solid is sent out After ferment 12 hours, ventilation oxygen supply air quantity is 0.1~0.3V/V.min, and ventilation oxygen supply time is, 8：00~17：00, fermentation ends When there is yeast aroma and lactic taste to produce；Scatter after ventilation, be sent on fluid bed and dry, 60~80 DEG C of EAT goes out 30~40 DEG C of air temperature, 8 minutes~12 minutes time of fluidisation, water content 12.0%~15.0%, crushing, the excessively mesh of grit number 80~ 100 mesh；Obtain chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder.

2. according to the method for the chrysanthemum meal fermenting and producing aquatic products aquatic products livestock and poultry active peptide powder described in claim 1, its feature exists In：

Chrysanthemum meal water content is that 10%, dregs of beans water content is that the dense bacterium paste dry powder water content of 10%, arginine is in fermentation raw material 10%th, Dried Corn Steep Liquor Powder water content is 10%；Total moisture content 10.0% in fermentation raw material；

PH value is 6.5~7.0 during fermentation ends, and total acid is 200~300 ° of T；

Product crude protein 50~60% during fermentation ends, wherein (short chain peptide molecular weight is containing short-chain peptide more than 20%~30% Below 2000 dalton), lysine 2.0%~3.0%, methionine 1.0%~2.0%, threonine 1.0%~2.0%, color Propylhomoserin 0.5%~0.9%, arginine 3.5%~4.0%.

3. according to the method for the chrysanthemum meal fermenting and producing aquatic products aquatic products livestock and poultry active peptide powder described in claim 2, its feature exists In：PH value is 7.0 during fermentation ends, and total acid is 200 ° of T；

Product crude protein 50% during fermentation ends, wherein, containing short-chain peptide more than 20.8%, (short chain peptide molecular weight is 2000 dongles It is following), lysine 2.16%, methionine 1.16%, threonine 1.26%, tryptophan 0.63%, arginine 3.6%.

4. according to the chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder production method described in claim 1, it is characterised in that： Various raw material weight ratios are in solid fermentation raw materials：50 parts of chrysanthemum meal, 10 parts of dregs of beans, 20 parts of arginine underflow bacterium solution dry powder, jade 20 parts of Rice ＆ peanut milk dry powder；In the solid fermentation process, fermentation raw material and the optimum weight ratio of microbial fermentation flora seed liquor be, 100 parts of fermentation raw material:10 parts of bacillus subtilis, 10 parts of arginine producing strain, 10 parts of ocean rhodotorula bacterium, Lactobacillus plantarum 10 Part.

5. according to the chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder method described in claim 1, it is characterised in that：It is described Liquid seeds culture and fermentation training is respectively adopted in bacillus subtilis, arginine producing strain, ocean rhodotorula, Lactobacillus plantarum Support；

Every milliliter is >=100 hundred million containing viable count in bacillus subtilis and the arginine producing strain seed liquor；Ocean rhodotorula Every milliliter is >=50 hundred million containing viable count in bacterium and Lactobacillus plantarum seed liquor.

6. according to the method described in claim 1 chrysanthemum meal fermenting and producing aquatic products aquatic products livestock and poultry active peptide powder, it is characterised in that：

The bacillus subtilis seed tank culture base：It is by weight percentage：Glucose 0.5%~1.0%, Dried Corn Steep Liquor Powder 3.0%~3.5%, ammonium sulfate 0.1%~0.2%, magnesium sulfate 0.1%~0.2%, dipotassium hydrogen phosphate 0.1%~0.2%, remaining Measure as water, pH6.8~7.0；Sterilising conditions：115~121 DEG C of temperature, 0.08~0.10MPa of pressure, 18~20min of time；Training The condition of supporting：Inoculum concentration 10~20%；Tank presses 0.01~0.10MPa, air quantity 100:100~100:200vvm, rotating speed 100~ 30 DEG C~32 DEG C of 200rpm, temperature；48~64h of time；

The arginine producing strain seed tank culture base：It is by weight percentage：Glucose 1.5%~2.0%, glutamic acid 1.5%~2.0%, Dried Corn Steep Liquor Powder 3.0%~3.5%, ammonium sulfate 0.1%~0.2%, magnesium sulfate 0.1%~0.2%, phosphorus Sour hydrogen dipotassium 0.1%~0.2%, surplus is water, pH6.8~7.0；Sterilising conditions：115~121 DEG C of temperature, pressure 0.08~ 0.10MPa, 18~20min of time；Condition of culture：Inoculum concentration 10~20%；Tank presses 0.01~0.10MPa, air quantity 100:100~ 100:200vvm, 100~200rpm of rotating speed, 30 DEG C~32 DEG C of temperature；48~64h of time；

The culture medium of the ocean rhodotorula bacterium seeding tank：It is by weight percentage：Glucose 3.0%~3.5%, dusty yeast 2.0%~2.5%, Dried Corn Steep Liquor Powder 2.0%~2.5%, ammonium sulfate 0.1%~0.2%, magnesium sulfate 0.1%~0.2%, phosphorus Sour hydrogen dipotassium 0.1%~0.2%, surplus is water, pH6.6~6.8；Sterilising conditions：115~121 DEG C of temperature, pressure 0.08~ 0.10MPa, 18~20min of time；Condition of culture：Inoculum concentration 10~20%；Tank presses 0.01~0.10MPa, air quantity 100:100~ 100:200vvm, 100~200rpm of rotating speed, 28 DEG C~30 DEG C of temperature；48~64h of time；

The culture medium of the Lactobacillus plantarum seeding tank：It is by weight percentage：Glucose 1.5~2.0%, dusty yeast 2.0%~2.5%, Dried Corn Steep Liquor Powder 2.0%~2.5%, ammonium sulfate 0.1%~0.2%, magnesium sulfate 0.1%~0.2%, phosphorus Sour hydrogen dipotassium 0.1%~0.2%, surplus is water, pH6.4~6.6；Sterilising conditions：115~121 DEG C of temperature, pressure 0.08~ 0.10MPa, 18~20min of time；Condition of culture：Inoculum concentration 10~20%；Tank presses 0.01~0.10MPa, air quantity 100:100~ 100:200vvm, 100~200rpm of rotating speed, 33 DEG C~35 DEG C of temperature；48~64h of time (being quiescent culture after 24 hours).

7. according to the chrysanthemum meal fermenting and producing aquatic products aquatic products livestock and poultry active peptide powder method described in claim 6, it is characterised in that：

The bacillus subtilis seed tank culture base：It is by weight percentage：Glucose 0.5%, Dried Corn Steep Liquor Powder 3.0%, Ammonium sulfate 0.1%, magnesium sulfate 0.1%, dipotassium hydrogen phosphate 0.1%, surplus are water, pH7.0；Sterilising conditions：121 DEG C of temperature, pressure Power 0.10MPa, time 20min；Condition of culture：Inoculum concentration 10%；Tank pressure 0.05MPa, air quantity 100:200vvm, rotating speed 32 DEG C of 200rpm, temperature；Time 48h；

The arginine producing strain seed tank culture base：It is by weight percentage：Glucose 2.0%, glutamic acid 2.0%th, Dried Corn Steep Liquor Powder 3.0%, ammonium sulfate 0.1%, magnesium sulfate 0.1%, dipotassium hydrogen phosphate 0.1%, surplus is water, pH6.8； Sterilising conditions：115 DEG C of temperature, pressure 0.08MPa, time 18min；Condition of culture：Inoculum concentration 10%；Tank pressure 0.05MPa, air quantity 100:200vvm, rotating speed 200rpm, 32 DEG C of temperature；Time 48h；

The ocean rhodotorula bacterium seeding tank culture medium：It is by weight percentage：Glucose 3.0%, dusty yeast 2.0%%, Dried Corn Steep Liquor Powder 2.0%, ammonium sulfate 0.1%, magnesium sulfate 0.%, dipotassium hydrogen phosphate 0.1%, surplus are water, pH6.8；Sterilize part： 115 DEG C of temperature, pressure 0.08MPa, time 18min；Condition of culture：Inoculum concentration 10%；Tank pressure 0.05MPa, air quantity 100: 200vvm, rotating speed 200rpm, 28 DEG C of temperature；Time 48h；

The culture medium of the Lactobacillus plantarum seeding tank：It is by weight percentage：Glucose 2.0%, dusty yeast 2.0%, jade Rice ＆ peanut milk dry powder 2.0%, ammonium sulfate 0.1%, magnesium sulfate 0.1%, dipotassium hydrogen phosphate 0.1%, surplus are water, pH6.6；Sterilize bar Part：115 DEG C of temperature, pressure 0.08MPa, time 18min；Condition of culture：Inoculum concentration 10；Tank pressure 0.05MPa, air quantity 100: 100vvm, rotating speed 100rpm, 35 DEG C of temperature；Time 48h (being quiescent culture after 24 hours).

8. in accordance with the method for claim 1, chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder method, its feature exists In：Solid fermentation material is passed through filtrated air in fermentation bed and carries out aerobic fermentation, and initial temperature is 35 DEG C, and fermentation temperature is 60 DEG C, fermentation time is 72 small；After solid fermentation 12 hours, ventilation oxygen supply air quantity is 0.1~0.3V/V.min, oxygen supply time of divulging information For 8：00~17：00, there are yeast aroma and lactic taste to produce during fermentation ends；Scatter after ventilation, be sent on fluid bed and dry Dry, 80 DEG C of EAT, 40 DEG C of leaving air temp fluidizes 12 minutes time, and water content 12.0% is crushed, crosses the mesh of grit number 100； Obtain chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder.

9. according to the chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder production method described in claim 1, it is characterised in that： In liquid fermentation, seeding tank is 0.1 ton tank 4, and fermentation tank is 1 ton tank 4, and arginine producing strain, withered grass gemma are produced respectively Bacillus, marine yeast, Lactobacillus plantarum.