CN105994941A - Non-antibiotic feed prepared through microbial fermentation - Google Patents
Non-antibiotic feed prepared through microbial fermentation Download PDFInfo
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- CN105994941A CN105994941A CN201610349400.2A CN201610349400A CN105994941A CN 105994941 A CN105994941 A CN 105994941A CN 201610349400 A CN201610349400 A CN 201610349400A CN 105994941 A CN105994941 A CN 105994941A
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- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 230000031891 intestinal absorption Effects 0.000 description 1
- XBDUTCVQJHJTQZ-UHFFFAOYSA-L iron(2+) sulfate monohydrate Chemical compound O.[Fe+2].[O-]S([O-])(=O)=O XBDUTCVQJHJTQZ-UHFFFAOYSA-L 0.000 description 1
- 229940017800 lactobacillus casei Drugs 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 230000007886 mutagenicity Effects 0.000 description 1
- 231100000299 mutagenicity Toxicity 0.000 description 1
- 239000004309 nisin Substances 0.000 description 1
- 235000010297 nisin Nutrition 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 210000001819 pancreatic juice Anatomy 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000004460 silage Substances 0.000 description 1
- ZFRKQXVRDFCRJG-UHFFFAOYSA-N skatole Natural products C1=CC=C2C(C)=CNC2=C1 ZFRKQXVRDFCRJG-UHFFFAOYSA-N 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 229940087562 sodium acetate trihydrate Drugs 0.000 description 1
- 239000004317 sodium nitrate Substances 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000000528 statistical test Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 1
- 239000001393 triammonium citrate Substances 0.000 description 1
- 235000011046 triammonium citrate Nutrition 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention relates to a non-antibiotic feed prepared by adding a mixture to a compound bacterium liquid and carrying out solid fermentation. The compound bacterium liquid is prepared by mixing 1-3 parts by weight of lactobacillus paracasei liquid, 1-3 parts by weight of aspergillus oryzae liquid and 1-3 parts by weight of candida utilis liquid. Compared with common pig feeds, the non-antibiotic feed has the advantages that the non-antibiotic feed is reasonable in compatibility, has obvious curative effects and can achieve the effect of obviously reducing the diarrhea rates of pigs; experiments prove that the non-antibiotic feed has special effects on improvement of diarrhea of pigs; without western medicines-antibiotics, the feed does not have toxic or side effect or drug resistance and is pollution-free.
Description
Technical field
The present invention relates to field of fodder, be specifically related to a kind of domestic animal nonreactive feedstuff and application thereof.
Background technology
Since mid-term in 20th century, feedstuff adds antibiotic to prevention Animal diseases, promotion growth of animal, raising feedstuff
The aspects such as remuneration, raising animal products yield have played positive effect.Along with extensive as feed additive of antibiotic
Application, its all drawback recognizes also with by people, such as causes animal gastrointestinal tract normal flora to be lacked of proper care, and produces drug resistance
Property etc..The life-time service of antibiotic causes remaining in a large number in its meat in animal productiong, egg product, and these residuals are to people
Body has toxicity, teratogenecity, carcinogenecity, mutagenicity.Not only the quality of livestock products reduces, and environment is caused brokenly by residual hazard
Bad, the health of the mankind as animal product consumer is constituted a threat to, the trade simultaneously also constituting livestock products outlet is inverse
Difference, brings difficulty to the foreign exchange earning of livestock products, affects the economic benefit of aquaculture self to a great extent.Seek
Looking for environmental type antibiotic substitute, alleviating the negative effect that Production of Livestock and Poultry brought by antibiotic becomes various countries' animal husbandry and compels
Be essential problem to be solved.
Probiotics preparation have have no side effect, have no drug resistance, the plurality of advantages such as noresidue and notable, the low cost of effect,
Can effectively supplement the beneficial microbe in digestive tract, kill the pathogen such as harmful microorganism especially escherichia coli, improvement disappears
Change road colony balance, improve rapidly the absorbability of body disease-resistant, metabolism and feedstuff, thus play preventing and treating digestive tract disease
With the double effects promoting growth.Probiotics preparation is with the model of action of its uniqueness, natural composition, it will be apparent that make
By effect, become preferable Substitutes For Antibiotic.
Probiotics preparation refers to, microbial state treatment theory, Tiny ecosystem nutrient theory theoretical at microecological balance and Tiny ecosystem preventing and treating
Under theoretical direction, through strict selection-breeding and domestication, biological engineering and Tiny ecosystem engineering prepare, animal body stomach can be passed through
The barriers such as acid, bile acid, pancreatic juice and survive, finally in digestive tract field planting and breed, adjust microbial state treatment in host,
Keep microecological balance, improve host health level or the probiotics of the state that improves health and metabolite thereof and growth stimulation
The goods of material.
Following several standards can must be fulfilled for: (1) first can not produce any toxin as the microorganism of probiotics preparation,
The most nontoxic, harmless, safety has no side effect；(2) the Digestion table to gastric acid, pepsin, trypsin and bile
Reveal certain resistance, thus can survive in digestive tract；(3) gastrointestinal mucosal can be adhered to, permanently or temporarily
It is colonizated in gastrointestinal tract to property；(4) may advantageously facilitate colony balance or prevention ecological disturbance in host, host health is had
Certain facilitation；(5) can have Clinical efficacy by Rational Dosage when its product is fed；(6) product preparation and storage
Higher active and longer stability can be kept during depositing；(7) can survive in multiple host.This mark last
Will definitely be most difficult to reach, although most of species have microbiologic population in metastable intestinal, but each species have again certainly
The bacterial community that oneself is unique.And, some factors, as pressure or antibiotic therapy can change microorganism in intestinal
Fauna, causes the population even pathogen accounting for time dominant advantage to become dominant advantage bacterium.Therefore, bacterial strain is selected to adapt to comprehensive
Consider, select to meet the bacterial strain of more conditions above as far as possible.
A lot of probiotic bacterias find out from original intestinal bacteria, and are proved some gastroenteropathys are had clinic
Effect.Except, in addition to clinical effect is different with composition, their mechanism of effect of probiotic bacteria is different.Due to probiotic bacteria system
Agent can supplement the microflora lacked in intestinal in quantity or kind, can adjust or maintain Tiny ecosystem in intestinal
Balance, strengthens immunologic function, promotes the digesting and assimilating of nutrient substance, thus reaches anosis diseases prevention, ill cures the disease, and improves
Word material conversion ratio and the effect of livestock and poultry production performance.So far about feed microbe additive research report result difference very
Greatly, the most also there are all explanations.By the experience to probiotics preparation institute accumulation in animal husbandry, existing will
The mechanism of action of probiotics preparation is summarized as follows:
1, intestinal microecology balance is maintained: there is substantial amounts of microorganism species at animal gastrointestinal tract, and keep relative equilibrium steady
Determine state.Some of which flora is significant to growth promoter and the resist the disease of animal.Under normal circumstances, animal
In intestinal, dominant population is anaerobe, accounts for more than 99%, including lactobacillus, bacillus bifidus, digestion bacillus and excellent
Bacillus etc., and aerobe and facultative anaerobe only account for 1%.These floras once lose balance, then make the digestive tract of animal
Technical ability gets muddled, and production performance declines, and pathogenic microbes can cause animal to fall ill by amount reproduction, now uses prebiotic
Bacteria preparation can effectively supplement the quantity of probiotics, the growth and breeding of suppression pathogenic flora, and the flora of adjustment and recovery intestinal is put down
Weighing apparatus.The mechanism that animal body maintains intestinal microecology balance has a following two aspect: (1) colonization resistance: animal gastrointestinal tract
Ancestral home kind group energy suppress other external microorganism field planting in intestinal, here it is colonization resistance, also cry competition exclusion.(2)
Produce antibacterial substance: such as lactobacillus can produce bacteriocin, bacterioid element and other there is the metabolite of bacteriostasis.Carefully
Rhzomorph is that the class synthesizing during bacterial metabolism and being secreted in environment has inhibitory action to the strain that sibship is nearer
Protide antibacterial substance.Most known bacteriocin only has a bacteriostasis to identical Gram-stained antibacterial, but along with
Research deeply, finds that many bacteriocin sphere of actions are the widest.
2, biology takes oxygen by force: the useful aerobe in probiotic bacteria during growth and breeding, consumes in environment in animal intestinal
Oxygen, it is possible to decrease field planting and the growth and breeding of the locally concentration of oxygen molecule, beneficially normal flora obligate anaerobe make intestinal micro-
Ecological balance recovers normal, reaches disease preventing and treating purpose.Result of study display dysentery piglet is administered orally waxy spore for three days on end
After bacillus, in enteral anaerobe, bacillus bifidus, lactobacillus dramatically increase, and escherichia coli, Salmonella substantially reduce.
After pig feeds Bacillus licheniformis, in intestinal microbial population, anaerobe increases, and aerobe and facultative anaerobe particularly large intestine bar
Bacterium substantially reduces.
3, Ant agonism: the probiotics in probiotics preparation has antagonism to pathogenic microorganism in vivo, i.e. field planting
Drag.This colonization resistance is because the adsorption site of internal microorganism and pathogenic bacterium competition gut epithelium and produces.As
Really these adsorption sites are by occupied by more beneficial microbe, and pathogenic microorganism will be ostracised, and discharge body with feces
Outward.Research report is pointed out with finding after bacillus cereus feeding chickling that chickling enteral escherichia coli and Salmonella are had the most aobvious by it
Write antagonism.In vitro in test tube culture assays, pathogenic S. pullonum is had by lactobacillus microbial ecological agent
The strongest biological antagonist.
4, the immunologic function of strong body: some probiotics preparation is good immunoactivator, because itself is with regard to right and wrong
Specific immunity regulatory factor, can stimulate body immune system to produce interferon, improve the concentration of immunoglobulin and huge bite
The activity of cell, makes the humoral immunization of animal and cellular immune level improve, the probiotics preparation raising to animal immunizing power
It is mainly manifested in the following aspects: (1) strengthens the activity of macrophage has result of study display lactic acid bacteria to be obviously enhanced
Macrophage activity.Cheese lactobacillus can improve macrophage to listerial phagocytic activity；Separately there are some researches show, ferment
Female extract can strengthen the reaction of multiple macrophage；(2) to immune organ, antibody produces level, cytokine produces
Impact；(3) activation and the potentiation to specific immune response.
5, synthesis digestive enzyme, promotes digesting and assimilating of nutrient substance: the beneficial microbe in probiotics preparation can produce hydrolysis
Enzyme, digestive enzyme etc., be conducive to the complicated carbohydrates such as the protein in degraded feedstuff, fat, improve food conversion
Rate.The aspergillosis such as aspergillus oryzae, aspergillus niger can produce cellulase, beneficially animal digestion, absorption and the profit to cellulose
With.Aspergillus niger can also produce amylase, protease, pectase.Some yeast has the effect of Rich in Trace Element, makes
The organic form being become animal absorption easy to digest by inorganic states form.Acid type benefit is produced with bacillus cereus and lactobacillus etc.
After raw bacterium feeding animals, animal mucous membrane of small intestine gauffer increases, and continuous hair lengthens, and mucous glands nest is deepened, and intestinal absorption area increases
Greatly, thus improve the utilization rate of rate of body weight gain and feedstuff.Bacillus subtilis and Bacillus licheniformis are except having stronger albumen
Outside enzyme, amylase and lipase active, the most also there is the enzyme of some complex carbohydrates in degrading plant feedstuff, as
Pectase, glucanase, cellulase etc., the enzyme that wherein a lot of enzymes are mammal and birds itself can not produce.
6, intestinal environment is improved, the generation of the minimizing harmful substance such as ammonia, amine: the materials such as ammonia, amine, indole, hydrogen sulfide
Animal intestinal mucomembranous cell there is obvious toxic action.Lactic acid bacterias etc. can produce organic acid, antibacterial at intestinal growth and breeding
The inhibiting substances such as element, can suppress the growth of the Putrefying bacterias such as enteral escherichia coli, reduce the activity of urase, and then reduce egg
White matter, to the conversion of the harmful substance such as ammonia, amine, makes the concentration of enteral ammonia reduce, and minimizing is outwardly drained, and improves poultry
The living environment of fowl.
Formally probiotics preparation is divided into probiotics, prebiotics and symphysis unit in the world.The probiotics i.e. probiotics preparation of narrow sense,
Refer to host harmless, active bacteria formulation and the metabolite thereof improving host intestine flora ecological balance effect can be played.Mesh
Before be applied to the probiotic bacteria of human body and have lactic acid bacteria, bacillus bifidus, enterococcus, escherichia coli, bacillus subtilis, lichens
Bacillus cereus, bacillus cereus, Clostridium butyricum and yeast etc..But prebiotics refers to be absorbed energy by Host Digestion
The material of one or more probiotics growth and breedings original in enough selectively promoting host intestine, by improving probiotics
Quantity, suppress harmful bacterial growth, thus reach adjust intestinal microbial population, reach intestinal microecology balance, promote body
Healthy purpose.Such as bifidus factor, oligosaccharide kind material or title oligosaccharide etc..Also has some Chinese herbal medicine of China, such as people
Ginseng, Radix Codonopsis, the Radix Astragali etc. or Folium Camelliae sinensis extract also can play the effect of prebiotics.Symphysis unit refers to include in preparation prebiotic
Element and the two-part preparation of prebiotics.This based article theoretically have concurrently above the two composition and the mixture of characteristic, but this
Definition is seldom adopted.
Both at home and abroad probiotic bacteria being generally segmented into three classes, the first kind is the general anaerobism i.e. Lactobacillus of oxytolerant, as addicted to acid
Lactobacillus, lactobacillus casei etc.；Equations of The Second Kind is the Bifidobacterium of strictly anaerobic, such as bifidobacterium longum, bifidobacterium breve
Deng；3rd class is amphimicrobian coccus, such as enterococcus faecalis, breast enterococcus etc..Additionally, also have some yeast with enzyme also
The category of probiotic bacteria can be included into.The probiotic bacteria being usually used in feedstuff includes lactic acid bacteria, bacillus cereus and yeast etc..
Lactic acid bacteria is the common name of the gram-positive bacterium that a group energy utilizes the fermentable carbohydrate a large amount of lactic acid of generation.Breast
Bacterium is defined as in acid: cell is shaft-like or spherical, and Gram-positive does not produce peroxidating oxygenase, consumption of glucose
And more than 50% produce lactic acid, be formed without Endospore, without mobility, or only minority has mobility.It leads in animal body
Cross biological antagonist, reduce pH value, stop and suppress the intrusion of pathogenic bacterium and field planting, degraded ammonia, indole, scatol etc. to have
Pest matter, maintains normal ecological balance in intestinal, strengthens humoral immunization and cellular immunization.Lactic acid bacteria can be used for suckling and
In the feedstuff of weaning stage animal.In nature, lactic acid bacteria is widely distributed, does not only exist the intestinal with humans and animals and other devices
In official, there is also and in food, feedstuff, soil, plant rhizosphere, river lake simultaneously.Lactic acid bacteria industry, agricultural,
Food and health care etc. have the highest using value in field closely-related with the mankind, can be used for probiotics preparation,
Lactic acid, silage inoculant bacteria, latic acid-fermented food (such as fermented dairy product, fermented vegetable, fermentation meat product etc.), bacteriocin,
The production of cosmetic humectant etc..At present the lactic acid bacteria of application arise primarily at Lactobacillus, nisin Pseudomonas and
The microorganism of nearly 30 kinds of Bifidobacterium.The Ministry of Agriculture of China allows the lactic acid bacteria being used as feed additive to have: cheese milk bar
Bacterium, Lactobacillus plantarum, streptococcus faecalis, streptococcus faecalis, pediococcus acidilactici, bacillus acidophilus, Streptococcus lactis (Lister) Lohnis 1909.554. etc. 7 kinds.
Bacillus cereus is the bacterium that passes by one's way of the intestinal for probiotics, it is impossible to be colonizated in intestinal.It is aerobic, harmless, can produce
Spore acid and alkali-resistance, high temperature resistant and extruding, intestinal acidity environment has the stability of height, promotes the growth of probiotics,
Harmful bacteria in antagonism intestinal, enhancing human body immunity power, improves resistance against diseases；More strongly active protease and starch can be secreted
Enzyme, can significantly improve animal growth rate and promote the digestion of feed nutrition material.Report that more strain has hay bud at present
The probiotics kinds such as spore bacillus, Bacillus licheniformis, bacillus cereus and Japan's bacillus cereus.
Yeast one for probiotics preparation is active yeast preparation, and one is yeast culture.Yeast cells is rich in egg
White matter, nucleic acid, vitamin and multiple enzyme, have enhancing animal immunizing power, improves feedstuff palatability, promotes that animal is to raising
The functions such as the abilities of digestive and absorption of material, and the animal utilization rate to phosphorus can be improved；Its culture is nutritious, rich in B race
Vitamin, mineral, digestive enzyme, somatomedin and more complete aminoacid.The application age of yeast and culture thereof
For a long time, being widely used in the cultivation of various animal, the current Ministry of Agriculture is allowed for the yeast of probiotics wine brewing
Yeast, beer yeast etc..
At present, using compounding to Lactobacillus paracasei, aspergillus oryzae and the Candida utilis application as pig nonreactive feed additive still
Have no report.
Summary of the invention
The technical problem to be solved is to provide one can be effectively improved diarrhea of pigs, and has no side effect, pollution-free,
The pig having no drug resistance nonreactive feedstuff.
The present invention solves the problems referred to above and be employed technical scheme comprise that, it is provided that nonreactive feedstuff prepared by a kind of fermentable, by
Batch adds composite bacteria liquid solid fermentation and makes, and wherein composite bacteria liquid is by Lactobacillus paracasei bacterium solution 1-3 weight portion, rice
Aspergillosis bacterium solution 1-3 weight portion and Candida utilis liquid 1-3 weight portion are mixed.
Preferably, described composite bacteria liquid by Lactobacillus paracasei bacterium solution 2 weight portion, aspergillus oryzae bacterium solution 1 weight portion and produces protein vacation
Silk yeast liquid 2 weight portion is compounding to be made.
It is furthermore preferred that viable count is identical in described three kinds of bacterium solution of Unit Weight part.
Preferably, described batch includes bean cake, Zein powder, rapeseed meal, Semen arachidis hypogaeae dregs, calcium hydrogen phosphate and Sal etc..
It is furthermore preferred that described batch also includes lysine, methionine and threonine etc..
It is furthermore preferred that described batch also includes vitamin A, vitamin C, vitamin E and vitamin B12Deng.
Most preferably, described batch by bean cake 50 weight portion, Zein powder 20 weight portion, rapeseed meal 10 weight portion,
Semen arachidis hypogaeae dregs 5 weight portion, calcium hydrogen phosphate 5 weight portion, Sal 5 weight portion, lysine 1 weight portion, methionine 1 weight
Part, threonine 1 weight portion, vitamin A 0.5 weight portion, vitamin C 0.5 weight portion, vitamin E 0.5 weight portion
And vitamin B120.5 weight portion mix homogeneously is made.
Preferably, described batch is (90-95) with the weight ratio of composite bacteria liquid: (10-5).
It is furthermore preferred that the weight ratio of described batch and composite bacteria liquid is 95:5.
The invention have the advantages that:
It is surprising that through repeated tests, present inventors have unexpectedly found that Lactobacillus paracasei bacterium solution, aspergillus oryzae
The nonreactive feedstuff obtained through solid fermentation after liquid and Candida utilis liquid are compounding has the effect of potentiation for preventing and treating diarrhea of pigs
Really, other active component using effects are coordinated more preferably.
Compared with common pig's feed, the nonreactive feedstuff compatibility of the present invention is reasonable, evident in efficacy, it is possible to significantly reduce diarrhea of pigs
Rate, experiment proves there is specially good effect to improving diarrhea of pigs；Due in feedstuff without western medicine antibiotics composition, the therefore feedstuff of the present invention
Have no side effect, have no drug resistance, pollution-free.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further described, but embodiments of the present invention are not limited to this.Following
Experimental technique used in embodiment if no special instructions, is conventional method.
Embodiment 1, the preparation of Lactobacillus paracasei bacterium solution
Lactobacillus paracasei (bacterial strain GMNL-33) is seeded to equipped with in the anaerobism pipe of MRS culture medium, 37 DEG C of perseverances
Temperature Anaerobic culturel 48h, obtains Lactobacillus paracasei bacterium solution.
Described MRS culture medium is: peptone 10.0g/L, Carnis Bovis seu Bubali cream 8.0g/L, yeast extract 4.0g/L, glucose 20.0g/L,
Tween 80 1ml/L, dipotassium hydrogen phosphate 2.0g/L, sodium acetate trihydrate 5.0g/L, Triammonium citrate 2.0g/L, seven water sulphuric acid
Magnesium 0.2g/L, manganese sulfate 0.05g/L, pH is 6.2-6.6.
Embodiment 2, the preparation of aspergillus oryzae bacterium solution
Aspergillus oryzae (bacterial strain CICC40188) is seeded on Czapek ' s agar culture medium, cultivates 72h for 28 DEG C,
Inoculate in Czapek ' s fluid medium, 28 DEG C of constant temperature culture 72h, obtain aspergillus oryzae bacterium solution.
Described Czapek ' s agar culture medium is: sucrose 3%, sodium nitrate 0.3%, bitter salt 0.05%, chlorination
Potassium 0.05%, four ferrous sulfate hydrate 0.001%, dipotassium hydrogen phosphate 0.1%, agar 1.5%, pH value 6.0-6.5.It is not added with
Agar is Czapek ' s fluid medium.
Embodiment 3, the preparation of Candida utilis liquid
Candida utilis (bacterial strain ATCC22023) is inoculated in wort agar culture medium, cultivates 72h for 28 DEG C,
Inoculate in malt juice liquid medium, 28 DEG C of constant temperature culture 72h, obtain Candida utilis liquid.
Described wort agar culture medium is: beerwort is 5-6 Baume degrees, and pH value is 6.5, adds 2g/100ml agar
Obtain.It is not added with agar and is malt juice liquid medium.
Embodiment 4, the preparation of batch
Take bean cake 50 weight portion, Zein powder 20 weight portion, rapeseed meal 10 weight portion, Semen arachidis hypogaeae dregs 5 weight portion, phosphorus
Acid hydrogen calcium 5 weight portion, Sal 5 weight portion, lysine 1 weight portion, methionine 1 weight portion, threonine 1 weight portion,
Vitamin A 0.5 weight portion, vitamin C 0.5 weight portion, vitamin E 0.5 weight portion and vitamin B120.5 weight portion
Mix homogeneously i.e. obtains batch.
Embodiment 5, the preparation (A1-A6) of nonreactive feedstuff
Lactobacillus paracasei bacterium solution cryoconcentration embodiment 1 prepared is about 10 to viable count in bacterium solution8Individual/g, presses
Inoculum concentration according to 5% joins in the batch that embodiment 4 prepares, and under the conditions of 35 DEG C, solid fermentation 7d, to obtain final product
Comparison nonreactive feedstuff A1.
Aspergillus oryzae bacterium solution cryoconcentration embodiment 2 prepared is about 10 to viable count in bacterium solution8Individual/g, according to 5%
Inoculum concentration join in the batch that embodiment 4 prepares, solid fermentation 7d under the conditions of 35 DEG C, obtain comparison nothing
Anti-feedstuff A2.
Candida utilis liquid cryoconcentration embodiment 3 prepared is about 10 to viable count in bacterium solution8Individual/g, presses
Inoculum concentration according to 5% joins in the batch that embodiment 4 prepares, and under the conditions of 35 DEG C, solid fermentation 7d, to obtain final product
Comparison nonreactive feedstuff A3.
Lactobacillus paracasei bacterium solution, aspergillus oryzae bacterium solution and the Candida utilis liquid low temperature that embodiment 1-3 is prepared
It is concentrated into viable count in three kinds of bacterium solution and is each about 108Individual/g, then it is mixed and made into composite bacteria liquid with the weight ratio of 2:2:1, according to
The inoculum concentration of 5% joins in the batch that embodiment 4 prepares, solid fermentation 7d under the conditions of 35 DEG C, obtains this
Invention nonreactive feedstuff A4.
Lactobacillus paracasei bacterium solution, aspergillus oryzae bacterium solution and the Candida utilis liquid low temperature that embodiment 1-3 is prepared
It is concentrated into viable count in three kinds of bacterium solution and is each about 108Individual/g, then it is mixed and made into composite bacteria liquid with the weight ratio of 1:2:2, according to
The inoculum concentration of 5% joins in the batch that embodiment 4 prepares, solid fermentation 7d under the conditions of 35 DEG C, obtains this
Invention nonreactive feedstuff A5.
Lactobacillus paracasei bacterium solution, aspergillus oryzae bacterium solution and the Candida utilis liquid low temperature that embodiment 1-3 is prepared
It is concentrated into viable count in three kinds of bacterium solution and is each about 108Individual/g, then it is mixed and made into composite bacteria liquid with the weight ratio of 2:1:2, according to
The inoculum concentration of 5% joins in the batch that embodiment 4 prepares, solid fermentation 7d under the conditions of 35 DEG C, obtains this
Invention nonreactive feedstuff A6.
The application in preventing and treating diarrhea of pigs of test example 1, nonreactive feedstuff of the present invention
For verifying the application effect of the present invention, the feed additive of the present invention tries in porkling healthy, that body weight is close
Test.Pig is divided into experimental group (A1-A6) and matched group, and often organizing is 50.Wherein, the daily ration of experimental group is for implementing
The nonreactive feedstuff A1-A6 that example 5 prepares, the daily ration of matched group is the batch of the identical weight of embodiment 4 preparation,
Each group pig is thrown something and fed 3 times every day, and free choice feeding in experimental period provides sufficient drinking-water simultaneously, and other every daily managements are arranged
Execute and carry out according to the Routine Management of plant, periodically carry out immunity and anthelmintic, after 30 days, detect the coliform count in feces
Amount, in the statistical test phase simultaneously, the incidence of pig in experimental group and matched group, calculates the diarrhea of pigs rate (being shown in Table 1) of each group.
Table 1
| Coliform count (107Individual/g) | Diarrhea rate | |
| Matched group | 5.87 | 24% |
| Experimental group A1 | 3.16 | 16% |
| Experimental group A2 | 3.08 | 16% |
| Experimental group A3 | 3.59 | 18% |
| Experimental group A4 | 2.44 | 12% |
| Experimental group A5 | 2.13 | 10% |
| Experimental group A6 | 1.07 | 4% |
By the contrast experiment of test example 1 it can be seen that the nonreactive Feed Energy of the present invention significantly reduces escherichia coli in piglet body
Quantity, reduces the chance of Infection in Piglets disease, reduces piglet sickness rate and diarrhea rate.Process of the test also finds feed
The piglet mental status of nonreactive feedstuff of the present invention is significantly better than matched group, and honey stomach, the smooth color and luster of fur is good.Additionally,
In the case of the holding of bacterium solution total amount is constant, by Lactobacillus paracasei bacterium solution, aspergillus oryzae bacterium solution and Candida utilis liquid
The anti-diarrhea of pigs effect of the nonreactive feedstuff that the compounding composite bacteria liquid made prepares is substantially better than single bacterium solution, wherein secondary cheese milk
The nonreactive feedstuff preventing and treating that bacillus bacterium solution, aspergillus oryzae bacterium solution and Candida utilis liquid are made after mixing with the weight ratio of 2:1:2
The effect of piglet diarrhea is the most prominent, and piglet diarrhea rate is reduced to 4%, creates and is difficult to intended excellent effect.
Claims (8)
1. the nonreactive feedstuff that prepared by fermentable, is added composite bacteria liquid by batch and makes through solid fermentation, and it is special
Levying and be, wherein composite bacteria liquid by Lactobacillus paracasei bacterium solution 1-3 weight portion, aspergillus oryzae bacterium solution 1-3 weight portion and produces protein vacation
Silk yeast liquid 1-3 weight portion is mixed.
Nonreactive feedstuff the most according to claim 1, it is characterised in that described composite bacteria liquid is by Lactobacillus paracasei bacterium
Liquid 2 weight portion, aspergillus oryzae bacterium solution 1 weight portion and Candida utilis liquid 2 weight portion are compounding to be made.
Nonreactive feedstuff the most according to claim 1, it is characterised in that described batch includes bean cake, zein
Powder, rapeseed meal, Semen arachidis hypogaeae dregs, calcium hydrogen phosphate and Sal etc..
Nonreactive feedstuff the most according to claim 3, it is characterised in that described batch also includes lysine, egg ammonia
Acid and threonine etc..
Nonreactive feedstuff the most according to claim 3, it is characterised in that described batch also includes vitamin A, dimension
Raw element C, vitamin E and vitamin B12Deng.
Nonreactive feedstuff the most according to claim 1, it is characterised in that described batch is by bean cake 50 weight portion, jade
Rice gluten powder 20 weight portion, rapeseed meal 10 weight portion, Semen arachidis hypogaeae dregs 5 weight portion, calcium hydrogen phosphate 5 weight portion, Sal 5
Weight portion, lysine 1 weight portion, methionine 1 weight portion, threonine 1 weight portion, vitamin A 0.5 weight portion, dimension
Raw element C 0.5 weight portion, vitamin E 0.5 weight portion and vitamin B120.5 weight portion mix homogeneously is made.
Nonreactive feedstuff the most according to claim 1, it is characterised in that described batch and the weight ratio of composite bacteria liquid
For (90-95): (10-5).
Nonreactive feedstuff the most according to claim 7, it is characterised in that described batch and the weight ratio of composite bacteria liquid
For 95:5.
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