CN102422996B - Preliminary microbial fermentation antibiotic-free feed for growth fattening pigs - Google Patents
Preliminary microbial fermentation antibiotic-free feed for growth fattening pigs Download PDFInfo
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- CN102422996B CN102422996B CN201110340383.3A CN201110340383A CN102422996B CN 102422996 B CN102422996 B CN 102422996B CN 201110340383 A CN201110340383 A CN 201110340383A CN 102422996 B CN102422996 B CN 102422996B
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Abstract
The invention discloses a preliminary microbial fermentation antibiotic-free feed for growth fattening pigs. The preliminary microbial fermentation antibiotic-free feed is formed by mixing a compound feed and a complex fermented feed, wherein a weight ratio of the compound feed to the complex fermented feed is (92-96):(4-8); the complex fermented feed is prepared by complexing the following fermented feeds in part by weight: 2.5 to 5.5 parts of lactobacillus acidophilus fermented feed, 0.05 to 0.30 part of bacillus subtilis fermented feed, 0.50 to 1.65 parts of saccharomyces cerevisiae fermented feed, 0.05 to 0.10 part of trichoderma longbrachiatum fermented feed, 0.05 to 0.20 part of aspergillus niger fermented feed and 0.05 to 0.25 part of aspergillus oryzae fermented feed. The preliminary microbial fermentation antibiotic-free feed for the growth fattening pigs has multiple varieties of beneficial bacteria and large number, contains various high-activity digestive enzymes such as beta-glucanase, xylanase, cellulose, protease and the like, exerts a good synergistic effect, improves the immunity of animal organisms and the microecological environment of intestinal tracts so as to improve the health condition of animals, improve the digestive utilization rate of the feed and improve the economic benefit of cultivation.
Description
Technical field
The present invention relates in technical field of animal, particularly preliminary microbial fermentation antibiotic-free feed for growth fattening pigs.
Background technology
20th century were added antibiotics in feed, had brought the develop rapidly of global livestock breeding industry.But the antibiotic feed of the feeding interpolation of Long term Animal, can produce antibody-resistant bacterium in vivo, and cause the problems such as the medicament residue of animal product.Along with the progressively raising of living standards of the people, consumer is transitioned into pursuit quality from pursuing quantity to the demand of animal products, more and more be restricted so add antibiotic way in feed, forbidden adding antibiotic in many countries in the areas such as Europe, North America, Asia in feed at present.
In addition, in feed product, add antibiotic in store pig early stage and can cause a series of adverse consequences:
1. add antibiotic meeting heavy damage pig rear intestinal microbiota, reduce the autoimmunity of pig, make pig for a long time in sub-health state, to most epidemic disease irresistances, and antibiotic can destroy intestinal mucosa, reduce the digestive utilization ratio of feed, affect the speed of growth of pig;
2. antibiotic can be residual in a large number in the meat of pig and internal organs, also can, along with fecaluria is excreted in natural environment, finally enter human body, harm humans health by food chain;
3. antibiotic abuse, causes that the antibody-resistant bacterium in environment produces in a large number, has a strong impact on the curative effect that uses antibiotic therapy animals and humans epidemic disease.
Develop antibiotic-free feed, solve the residual problem of medicine from source, become the key element of our times meat, egg, the dairy products market competitiveness.At present, carry out the research of a large amount of Substitutes For Antibiotics both at home and abroad, wherein microorganism fermentation nonreactive feed efficiency the best.
Summary of the invention
Technical problem to be solved by this invention is in feed product, to add the problem that antibiotic brings in store pig early stage and a kind of preliminary microbial fermentation antibiotic-free feed for growth fattening pigs is provided for existing, this preliminary microbial fermentation antibiotic-free feed for growth fattening pigs is rich in useful viable bacteria, polysaccharide, antibacterial peptide, digestive ferment, organic acid and somatomedin etc., thereby it is conducive to feed intake, digestion and absorption, can in antibiotic situation, improve grow-finish pig growth performance and resistance against diseases not adding, improve culture benefit.
Technical problem to be solved by this invention can be achieved through the following technical solutions:
Preliminary microbial fermentation antibiotic-free feed for growth fattening pigs, mixed by batch and composite fermentation material, wherein batch with the ratio of the weight of composite fermentation material is: 92～96: 4～8, and composite fermentation material is by composite the forming of fermentation material of following parts by weight: 2.5～5.5 parts of lactobacillus acidophilus fermentation materials, 0.05～0.30 part, fermentation of bacillus subtilis material, 0.50～1.65 part, fermentation by saccharomyces cerevisiae material, 0.05～0.10 part of the mould fermentation material of long handle wood, 0.05～0.20 part, fermentation of Aspergillus niger material, 0.05～0.25 part of aspergillus oryzae fermentation material.
Described batch is formulated by the raw material of following weight portion: 50～75 parts of corns, 0～8 part, wheat bran, 10～30 parts of dregs of beans, 0～6 part of imported fish meal, 0～5 part of soybean oil, 0.5～1 part of stone flour, 0.4～2 part of calcium monohydrogen phosphate, 0.2～0.6 part of salt, 0.07～0.5 part of lysine, 3～8 parts of composite fermentation material, 1 part of premix, premix is the raw material composition of following weight portion proportioning: 0.05～0.2 part of vitamin A, vitamin D
30.001～0.1 part, 0.7～6 part of vitamin E, 15～30 parts of vitamin Cs, Cobastab
120.001～0.003 part, Cobastab
60.13～0.38 part, 0.25～1 part, riboflavin, 0.7～1.5 part of calcium pantothenate, 2～3 parts, nicotinic acid, 0.005～0.05 part of biotin, 0.03～0.06 part, folic acid, 70～295 parts of Choline Chlorides, 17～249 parts, ferrous sulfate, 1.6～60 parts, copper sulphate, 0.7～47 part of manganese sulfate, 15～43 parts, zinc sulfate, 0.019～1.1 part of KI, 0.04～0.1 part of sodium selenite, 10～40 parts of antioxidants, 60～100 parts of mould inhibitors, 10～40 parts of sweeteners, 50～200 parts of system chaffs, 50～400 parts of zeolite powders.
Preliminary microbial fermentation antibiotic-free feed for growth fattening pigs of the present invention, on the basis of conventional compound feed prescription that meets store pig nutritional need in early stage, with the application number of the applicant application be 201010148491.6, disclosed lactobacillus acidophilus fermentation material preparation method in description in disclosed microbial fermentation antibiotic-free feed of application publication number CN1018286634A and preparation method thereof, fermentation of bacillus subtilis material preparation method, fermentation by saccharomyces cerevisiae material preparation method, the mould fermentation material preparation method of long handle wood, fermentation of Aspergillus niger material preparation method, the lactobacillus acidophilus fermentation material that aspergillus oryzae fermentation material preparation method is made, fermentation of bacillus subtilis material, fermentation by saccharomyces cerevisiae material, the mould fermentation material of long handle wood, fermentation of Aspergillus niger material unitary agent, according to the concrete physilogical characteristics of store pig and the composite fermented product of demand, replace the antibiotic in original formulation, phagostimulant, enzyme preparation, probiotic, mould inhibitors etc. form.
The store pig fermentative feedstuff of microbe beneficial bacterium in early stage that the present invention obtains is wide in variety, quantity is large, also contain the multiple high activity digestive ferments such as 1,4 beta-glucanase, zytase, cellulase, protease simultaneously, play good synergy, can improve animal body immunity, improve intestine microenvironment, thereby improve animal health condition, and improve feed digestive utilization ratio, promoted fanning economics.
Detailed description of the invention
In order to be illustrated more clearly in the present invention, enumerate following examples, but it is without any restrictions to scope of invention.
Embodiment 1
Store pig early stage, fermentative feedstuff of microbe, was mixed by batch and composite fermentation material, and wherein batch with the ratio of the weight of composite fermentation material is: 95: 5.
Batch is formulated by the raw material of following weight portion: 63.62 parts of corns, 21.5 parts of dregs of beans, 5 parts, wheat bran, 1 part of imported fish meal, 1 part of soybean oil, 0.8 part of stone flour, 0.65 part of calcium monohydrogen phosphate, 0.35 part of salt, 0.08 part of lysine, 1 part of premix.Premix is 0.1 part of vitamin A, vitamin D
30.045 part, 1 part of vitamin E, 18 parts of vitamin Cs, Cobastab
120.002 part, Cobastab
60.2 part, 0.5 part, riboflavin, 1 part of calcium pantothenate, 2 parts, nicotinic acid, 0.01 part of biotin, 0.04 part, folic acid, 150 parts of Choline Chlorides, 60 parts, ferrous sulfate, 50 parts, copper sulphate, 20 parts of manganese sulfates, 40 parts, zinc sulfate, 0.053 part of KI, 0.05 part of sodium selenite, 20 parts of antioxidants, 80 parts of mould inhibitors, 10 parts of sweeteners, 200 parts of chaffs of system, 347 parts of zeolite powders.
Composite fermentation material is by composite the forming of fermentation material of following parts by weight: 3 parts of lactobacillus acidophilus fermentation materials, 0.2 part, fermentation of bacillus subtilis material, 1.4 parts, fermentation by saccharomyces cerevisiae material, 0.1 part, the mould material of long handle wood, 0.1 part, fermentation of Aspergillus niger material, 0.2 part of aspergillus oryzae fermentation material.
The disclosed method of embodiment 2 in the description detailed description of the invention of lactobacillus acidophilus fermentation material, fermentation of bacillus subtilis material, fermentation by saccharomyces cerevisiae material, the mould fermentation material of long handle wood, fermentation of Aspergillus niger material, the disclosed microbial fermentation antibiotic-free feed of aspergillus oryzae fermentation material employing CN1018286634A is prepared from.
Embodiment 2
Preliminary microbial fermentation antibiotic-free feed for growth fattening pigs, is mixed by batch and composite fermentation material, and wherein batch with the ratio of the weight of composite fermentation material is: 94: 6.
Batch is formulated by the raw material of following weight portion: 66.16 parts of corns, 3 parts, wheat bran, 21 parts of dregs of beans, 1 part of imported fish meal, 0.75 part of stone flour, 0.6 part of calcium monohydrogen phosphate, 0.4 part of salt, 0.09 part of lysine, 1 part of premix; Premix is 0.15 part of vitamin A, vitamin D
30.055 part, 2 parts of vitamin Es, 25 parts of vitamin Cs, Cobastab
120.003 part, Cobastab
60.38 part, 0.6 part, riboflavin, 0.8 part of calcium pantothenate, 3 parts, nicotinic acid, 0.02 part of biotin, 0.05 part, folic acid, 180 parts of Choline Chlorides, 70 parts, ferrous sulfate, 40 parts, copper sulphate, 25 parts of manganese sulfates, 30 parts, zinc sulfate, 0.062 part of KI, 0.06 part of sodium selenite, 25 parts of antioxidants, 70 parts of mould inhibitors, 15 parts of sweeteners, 150 parts of chaffs of system, 362.82 parts of zeolite powders.
Composite fermentation material is by composite the forming of fermentation material of following parts by weight: 4 parts of lactobacillus acidophilus fermentation materials, 0.3 part, fermentation of bacillus subtilis material, 1.2 parts, fermentation by saccharomyces cerevisiae material, 0.2 part, the mould material of long handle wood, 0.15 part, fermentation of Aspergillus niger material, 0.15 part of aspergillus oryzae fermentation material.
The disclosed method of embodiment 2 in the description detailed description of the invention of lactobacillus acidophilus fermentation material, fermentation of bacillus subtilis material, fermentation by saccharomyces cerevisiae material, the mould fermentation material of long handle wood, fermentation of Aspergillus niger material, the disclosed microbial fermentation antibiotic-free feed of aspergillus oryzae fermentation material employing CN1018286634A is prepared from.
Embodiment 3
Store pig early stage, fermentative feedstuff of microbe, was mixed by batch and composite fermentation material, and wherein batch with the ratio of the weight of composite fermentation material is: 96: 4.
Batch is formulated by the raw material of following weight portion: 64.62 parts of corns, 21.5 parts of dregs of beans, 5 parts, wheat bran, 1 part of imported fish meal, 1 part of soybean oil, 0.8 part of stone flour, 0.65 part of calcium monohydrogen phosphate, 0.35 part of salt, 0.08 part of lysine, 1 part of premix.Premix is 0.12 part of vitamin A, vitamin D
30.05 part, 1.5 parts of vitamin Es, 20 parts of vitamin Cs, Cobastab
120.002 part, Cobastab
60.25 part, 0.55 part, riboflavin, 1 part of calcium pantothenate, 2.5 parts, nicotinic acid, 0.01 part of biotin, 0.05 part, folic acid, 200 parts of Choline Chlorides, 65 parts, ferrous sulfate, 45 parts, copper sulphate, 20 parts of manganese sulfates, 35 parts, zinc sulfate, 0.058 part of KI, 0.05 part of sodium selenite, 30 parts of antioxidants, 60 parts of mould inhibitors, 12 parts of sweeteners, 180 parts of chaffs of system, 326.86 parts of zeolite powders.
Composite fermentation material is by composite the forming of fermentation material of following parts by weight: 3 parts of lactobacillus acidophilus fermentation materials, 0.25 part, fermentation of bacillus subtilis material, 0.58 part, fermentation by saccharomyces cerevisiae material, 0.12 part, the mould material of long handle wood, 0.12 part, fermentation of Aspergillus niger material, 0.18 part of aspergillus oryzae fermentation material.
The disclosed method of embodiment 2 in the description detailed description of the invention of lactobacillus acidophilus fermentation material, fermentation of bacillus subtilis material, fermentation by saccharomyces cerevisiae material, the mould fermentation material of long handle wood, fermentation of Aspergillus niger material, the disclosed microbial fermentation antibiotic-free feed of aspergillus oryzae fermentation material employing CN1018286634A is prepared from.
Test detects data
Choose 240 of body weight 40kg left and right, the good DLY store pigs of the uniformity, male and female half and half, be divided at random 4 groups, establish 5 repetitions for every group, wherein 3 groups is test group, feeds according to the preliminary microbial fermentation antibiotic-free feed for growth fattening pigs of the embodiment of the present invention 1,2,3 preparations, and another group is control group, the routine that the calorie-protein of feeding is suitable has anti-feed, and experimental period is 35 days; When off-test, obtain data as shown in the table:
Above test data shows: test group preliminary microbial fermentation antibiotic-free feed for growth fattening pigs can effectively improve piglet feed intake and daily gain, reduces feedstuff-meat ratio and illness rate; In process of the test, also can be observed the advantage such as test group piglet is good compared with control group spiritedness state, and fur is smooth, and color and luster is good.
Claims (1)
1. preliminary microbial fermentation antibiotic-free feed for growth fattening pigs, is characterized in that, is mixed by batch and composite fermentation material, and wherein batch with the ratio of the weight of composite fermentation material is: 94: 6;
Batch is formulated by the raw material of following weight portion: 66.16 parts of corns, 3 parts, wheat bran, 21 parts of dregs of beans, 1 part of imported fish meal, 0.75 part of stone flour, 0.6 part of calcium monohydrogen phosphate, 0.4 part of salt, 0.09 part of lysine, 1 part of premix; Premix is 0.15 part of vitamin A, vitamin D
30.055 part, vitamin E2 part, 5 parts of Catergens, Cobastab
120.003 part, Cobastab
60.38 part, 0.6 part, riboflavin, 0.8 part of calcium pantothenate, 3 parts, nicotinic acid, 0.02 part of biotin, 0.05 part, folic acid, 180 parts of Choline Chlorides, 70 parts, ferrous sulfate, 40 parts, copper sulphate, 25 parts of manganese sulfates, 30 parts, zinc sulfate, 0.062 part of KI, 0.06 part of sodium selenite, 25 parts of antioxidants, 70 parts of mould inhibitors, 15 parts of sweeteners, 150 parts of chaffs of system, 362.82 parts of zeolite powders;
Composite fermentation material is by composite the forming of fermentation material of following parts by weight: 4 parts of lactobacillus acidophilus fermentation materials, 0.3 part, fermentation of bacillus subtilis material, 1.2 parts, fermentation by saccharomyces cerevisiae material, 0.2 part of the mould fermentation material of long handle wood, 0.15 part, fermentation of Aspergillus niger material, 0.15 part of aspergillus oryzae fermentation material;
The preparation method of described lactobacillus acidophilus fermentation material, fermentation of bacillus subtilis material, fermentation by saccharomyces cerevisiae material, the mould fermentation material of long handle wood, fermentation of Aspergillus niger material, aspergillus oryzae fermentation material, comprises the steps:
(1), prepare bacterial classification: get that lactobacillus acidophilus, bacillus subtilis, saccharomyces cerevisiae, long handle wood are mould, aspergillus niger, aspergillus oryzae be the bacterium that sets out, heat step by step respectively domestication, choosing carry, rejuvenation, obtain reaching the object bacterial classification of resistance to pelleting temperature, then spreading cultivation respectively and making bacteria containing amount is 1 × 10
7～1 × 10
8each strain liquid of individual/g, obtains lactobacillus acidophilus kind liquid, bacillus subtilis bacterial classification liquid, saccharomyces cerevisiae kind liquid, mould kind of liquid of long handle wood, aspergillus niger kind liquid, aspergillus oryzae kind liquid;
Above-mentioned bacterial classification can buy from Chinese Research for Industrial Microbial Germ preservation administrative center:
Lactobacillus acidophilus Lactobacil lus acidophi lus CICC6090;
Bacillus subtilis Bacillus subtilis CICC20037;
Saccharomyces Cerevisiae in S accharomyces cerevisiae CICC31015;
Aspergillus niger Aspergillus niger CICC40613;
Aspergillus oryzae Aspergi llus oryzae CICC40118;
The mould Trichoderma longibrachitun TB9702 of long handle wood;
(2) make fermentation material:
(2.1) make lactobacillus acidophilus fermentation material
A, making lactobacillus acidophilus fermentation medium
Get the raw material of following weight portion proportioning: 1～2 part of 15～20 parts of corn flour, 40～50 parts, wheat bran, 8～20 parts of bean cake powders, 3～8 parts, Cottonseed Meal powder, 8～20 parts of corn germ cakes, yeast extract powder l～3 part, 5～15 parts of corn steep liquors that contain 70～90% moisture content, 5～15 parts, molasses that contain 40～60% moisture content, stone flour l～2 part, calcium monohydrogen phosphate; The raw material of obtaining is put to mixer, added water, making raw material and the ratio of the weight of the water adding is 70～60: 30～40, stirs, and obtains compound material; Compound material is moved in high-pressure steam sterilizing pan to sterilizing 30～50 minutes under 0.1～0.12Mpa air pressure; Then compound material is taken the dish out of the pot, under gnotobasis, material temperature degree to be composite is down to 25～40 DEG C, obtains lactobacillus acidophilus culture medium;
B, cultivation lactobacillus acidophilus
Above-mentioned lactobacillus acidophilus kind liquid is joined in lactobacillus acidophilus culture medium and inoculated, the lactobacillus acidophilus kind liquid wherein adding is 1.5～2.5: 98.5～97.5 with the ratio of the weight of lactobacillus acidophilus culture medium, be to cultivate 6～8 days under the anaerobic environment of 35～42 DEG C in temperature, treat that viable count is greater than 1 × 10
8individual/when g, then be dried to water content≤12% under 45～50 DEG C of low temperature environments, then pack, keep in Dark Place, obtain lactobacillus acidophilus fermentation material;
(2.2) make fermentation of bacillus subtilis material
A, making fermentation of bacillus subtilis culture medium
Get the raw material of following weight portion proportioning: 15～25 parts of corn flour, 30～50 parts, wheat bran, 5～15 parts of bean cake powders, 5～15 parts, Cottonseed Meal powder, 10～20 parts of corn germ cakes, 2～5 parts of corn steep liquors that contain 70～90% moisture content, 2～8 parts, molasses that contain 40～60% moisture content; The raw material of obtaining is put to mixer, added water, making raw material and the ratio of the weight of the water adding is 70～60: 30～40, stirs, and obtains compound material; Compound material is moved in high-pressure steam sterilizing pan to sterilizing 30～50 minutes under 0.1～0.12Mpa air pressure; Then compound material is taken the dish out of the pot, under gnotobasis, material temperature degree to be composite is down to 25～40 DEG C, obtains bacillus subtilis bacterium culture medium;
B, cultivation bacillus subtilis
Above-mentioned bacillus subtilis bacterial classification liquid is joined in bacillus subtilis bacterium culture medium and inoculated, the bacillus subtilis bacterial classification liquid wherein adding is 1.5～2.5: 98.5～97.5 with the ratio of the weight of bacillus subtilis bacterium culture medium, be to cultivate 2～3 days under the oxygen environment of 35～42 DEG C in temperature, treat that viable count is greater than 1 × 10
9individual/gram, xylanase activity is greater than 1.2 × 10
4when u/g, then be dried to water content≤12% under 45～50 DEG C of low temperature environments, then pack, keep in Dark Place, obtain fermentation of bacillus subtilis material;
(2.3) make fermentation by saccharomyces cerevisiae material
A, making fermentation by saccharomyces cerevisiae culture medium
Get the raw material of following weight portion proportioning: 10～20 parts of corn flour, 20～40 parts, wheat bran, 3～8 parts of bean cake powders, 8～16 parts, Cottonseed Meal powder, 15～25 parts of corn germ cakes, 5～15 parts, inferior powder, 2～5 parts of corn steep liquors that contain 70～90% moisture content, 2～8 parts, molasses that contain 40～60% moisture content; The raw material of obtaining is put to mixer, added water, making raw material and the ratio of the weight of the water adding is 70～60: 30～40, stirs, and obtains compound material; Compound material is moved in high-pressure steam sterilizing pan to sterilizing 30～50 minutes under 0.1～0.12Mpa air pressure; Then compound material is taken the dish out of the pot, under gnotobasis, material temperature degree to be composite is down to 25～40 DEG C, obtains saccharomyces cerevisiae culture medium;
B, cultivation saccharomyces cerevisiae
Above-mentioned saccharomyces cerevisiae kind liquid is joined in saccharomyces cerevisiae culture medium and inoculated, the saccharomyces cerevisiae kind liquid wherein adding is 1.5～2.5: 98.5～97.5 with the ratio of the weight of saccharomyces cerevisiae culture medium, be to cultivate 2～3 days under the oxygen environment of 35～42 DEG C in temperature, treat that viable count is greater than 1 × 10
9individual/when g, then be dried to water content≤12% under 45～50 DEG C of low temperature environments, then pack, keep in Dark Place, obtain fermentation by saccharomyces cerevisiae material;
(2.4) make the mould fermentation material of long handle wood
A, the mould fermentation medium of making long handle wood
Get the raw material of following weight portion proportioning: 5～15 parts of corn flour, 30～50 parts, wheat bran, 3～8 parts of bean cake powders, 10～20 parts, Cottonseed Meal powder, 17～27 parts of corn germ cakes, 2～5 parts of corn steep liquors that contain 70～90% moisture content, 2～8 parts, molasses that contain 40～60% moisture content; The raw material of obtaining is put to mixer, added water, making raw material and the ratio of the weight of the water adding is 65～55: 35～45, stirs, and obtains compound material; Compound material is moved in high-pressure steam sterilizing pan to sterilizing 30～50 minutes under 0.1～0.12Mpa air pressure; Then compound material is taken the dish out of the pot, under gnotobasis, material temperature degree to be composite is down to 25～40 DEG C, obtains the mould culture medium of long handle wood;
B, to cultivate long handle wood mould
Mould kind of liquid of above-mentioned long handle wood is joined in the mould culture medium of long handle wood and inoculated, the mould kind of liquid of long handle wood wherein adding is 1.5～2.5: 98.5～97.5 with the ratio of the weight of the mould culture medium of long handle wood, be to cultivate 2～3 days under the oxygen environment of 35～42 DEG C in temperature, in the time that cellulase activity is greater than 1200u/g, under 45～50 DEG C of low temperature environments, be dried to water content≤12% again, then pack, keep in Dark Place, obtain the mould fermentation material of long handle wood;
(2.5) make fermentation of Aspergillus niger material
A, making fermentation of Aspergillus niger culture medium
Get the raw material of following weight portion proportioning: 10～20 parts of corn flour, 30～50 parts, wheat bran, 5～15 parts of bean cake powders, 5～15 parts, Cottonseed Meal powder, 12～22 parts of corn germ cakes, 2～5 parts of corn steep liquors that contain 70～90% moisture content, 2～8 parts, molasses that contain 40～60% moisture content; The raw material of obtaining is put to mixer, added water, making raw material and the ratio of the weight of the water adding is 65～55: 35～45, stirs, and obtains compound material; Compound material is moved in high-pressure steam sterilizing pan to sterilizing 30～50 minutes under 0.1～0.12Mpa air pressure; Then compound material is taken the dish out of the pot, under gnotobasis, material temperature degree to be composite is down to 25～40 DEG C, obtains aspergillus niger culture medium;
B, cultivation aspergillus niger
Above-mentioned aspergillus niger kind liquid is joined in aspergillus niger culture medium and inoculated, the aspergillus niger kind liquid wherein adding is 1.5～2.5: 98.5～97.5 with the ratio of the weight of aspergillus niger culture medium, be to cultivate 2～3 days under the oxygen environment of 35～42 DEG C in temperature, in the time that 1,4 beta-glucanase activity is greater than 10000u/g, under 45～50 DEG C of low temperature environments, be dried to water content≤12% again, then pack, keep in Dark Place, obtain fermentation of Aspergillus niger material;
(2.6) make aspergillus oryzae fermentation material
A, making aspergillus oryzae fermentation medium
Get the raw material of following weight portion proportioning: 10～20 parts of corn flour, 30～50 parts, wheat bran, 5～15 parts of bean cake powders, 5～15 parts, Cottonseed Meal powder, 12～22 parts of corn germ cakes, 2～5 parts of corn steep liquors that contain 70～90% moisture content, 2～8 parts, molasses that contain 40～60% moisture content; The raw material of obtaining is put to mixer, added water, making raw material and the ratio of the weight of the water adding is 65～55: 35～45, stirs, and obtains compound material; Compound material is moved in high-pressure steam sterilizing pan to sterilizing 30～50 minutes under 0.1～0.12Mpa air pressure; Then compound material is taken the dish out of the pot, under gnotobasis, material temperature degree to be composite is down to 25～40 DEG C, obtains aspergillus oryzae culture medium;
B, cultivation aspergillus oryzae
Above-mentioned aspergillus oryzae kind liquid is joined in aspergillus oryzae culture medium and inoculated, the aspergillus oryzae kind liquid wherein adding is 1.5～2.5: 98.5～97.5 with the ratio of the weight of aspergillus oryzae culture medium, be to cultivate 2～3 days under the oxygen environment of 35～42 DEG C in temperature, in the time that proteinase activity is greater than 1500u/g, under 45～50 DEG C of low temperature environments, be dried to water content≤12% again, then pack, keep in Dark Place, obtain aspergillus oryzae fermentation material.
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| US20150216203A1 (en) * | 2012-08-03 | 2015-08-06 | DuPont Nutritional Biosciences APS | Feed additive composition |
| CN103598461A (en) * | 2013-10-28 | 2014-02-26 | 郭敦福 | Big pig forage |
| CN103798541B (en) * | 2013-12-21 | 2016-08-03 | 河南旭瑞食品有限公司 | The fattening period pig feed of antibiotic-free |
| CN104012777A (en) * | 2014-05-13 | 2014-09-03 | 湖南山河美生物环保科技股份有限公司 | Microbial additive for removing peculiar smell of pets, and preparation method thereof |
| CN104222521A (en) * | 2014-09-23 | 2014-12-24 | 山东滨州智晟科技创新促进中心 | Growing-finishing pig feed containing D-ribose feed additive |
| CN104381695A (en) * | 2014-12-01 | 2015-03-04 | 郑州市大北农饲料科技有限公司 | Concentrated feed for early stage of growing-finishing pigs |
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| CN107156484A (en) * | 2017-05-26 | 2017-09-15 | 山东金码头生物科技有限公司 | A kind of interim pig feed |
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| CN101120731A (en) * | 2007-09-07 | 2008-02-13 | 济南和美华饲料有限公司 | Feed for improving sow oestrum and increasing farrowing amount |
| CN101828634A (en) * | 2010-04-16 | 2010-09-15 | 湖南创新生物科技有限公司 | Microbial fermentation antibiotic-free feed and preparing method thereof |
| CN101904442A (en) * | 2010-08-02 | 2010-12-08 | 河南普爱饲料股份有限公司 | Compound feed for first-born nursing sow |
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2011
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101120731A (en) * | 2007-09-07 | 2008-02-13 | 济南和美华饲料有限公司 | Feed for improving sow oestrum and increasing farrowing amount |
| CN101828634A (en) * | 2010-04-16 | 2010-09-15 | 湖南创新生物科技有限公司 | Microbial fermentation antibiotic-free feed and preparing method thereof |
| CN101904442A (en) * | 2010-08-02 | 2010-12-08 | 河南普爱饲料股份有限公司 | Compound feed for first-born nursing sow |
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