CN105994941B - A kind of nonreactive feed of microbial fermentation preparation - Google Patents
A kind of nonreactive feed of microbial fermentation preparation Download PDFInfo
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- 210000004907 gland Anatomy 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 235000019626 lipase activity Nutrition 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 230000007886 mutagenicity Effects 0.000 description 1
- 231100000299 mutagenicity Toxicity 0.000 description 1
- 239000004309 nisin Substances 0.000 description 1
- 235000010297 nisin Nutrition 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000000242 pagocytic effect Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 210000001819 pancreatic juice Anatomy 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Substances OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000004460 silage Substances 0.000 description 1
- ZFRKQXVRDFCRJG-UHFFFAOYSA-N skatole Natural products C1=CC=C2C(C)=CNC2=C1 ZFRKQXVRDFCRJG-UHFFFAOYSA-N 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 229940087562 sodium acetate trihydrate Drugs 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- 239000004317 sodium nitrate Substances 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000000528 statistical test Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 229940092665 tea leaf extract Drugs 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 1
- 239000001393 triammonium citrate Substances 0.000 description 1
- 235000011046 triammonium citrate Nutrition 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention relates to composite bacteria liquid is added through nonreactive feed made of solid state fermentation batch, wherein composite bacteria liquid is mixed by lactobacillus paracasei bacterium solution 1-3 parts by weight, aspergillus oryzae bacterium solution 1-3 parts by weight and candida utili bacterium solution 1-3 parts by weight.Compared with common pig's feed, nonreactive feed compatibility of the invention is reasonable, significant in efficacy, can significantly reduce diarrhea of pigs rate, experiments have shown that having special efficacy to diarrhea of pigs is improved；Since, without western medicine antibiotics ingredient, feed of the invention has no toxic side effect, has no drug resistance, pollution-free in feed.
Description
Technical field
The present invention relates to field of fodder, and in particular to a kind of domestic animal nonreactive feed and its application.
Background technique
Since 20th century mid-terms, added in feed antibiotic to prevention Animal diseases, promote growth of animal, improve feed
Remuneration, raising animal products yield etc. have played positive effect.With antibiotic answering extensively as feed addictive
With various drawbacks recognize also with by people, for example animal gastrointestinal tract normal flora is caused to be lacked of proper care, and generate drug resistance etc..
The long-time service of antibiotic leads to its meat in animal productiong, largely remains in egg product, these residuals have poison to human body
Property, teratogenesis, carcinogenicity, mutagenicity.Not only the quality of livestock products reduces, and residual hazard damages environment, to as animal
The health of the mankind of products customers constitutes a threat to, while also constituting the unfavourable trade balance of livestock products outlet, to going out for livestock products
Mouth, which is earned foreign exchange, brings difficulty, largely affects the economic benefit of aquaculture itself.Find environmental type antibiotic
Substitute, mitigating antibiotic becomes various countries' animal husbandry problem in the urgent need to address to the negative effect of Production of Livestock and Poultry bring.
Probiotics preparation have have no toxic side effect, have no drug resistance, noresidue and significant effect, at low cost etc. it is many excellent
Point can effectively supplement beneficial microbe in the digestive tract, kill pathogens, the improvement such as harmful microorganism especially Escherichia coli and disappear
Change road colony balance, improve the absorbability of body disease-resistant, metabolism and feed rapidly, to play prevention and treatment disease of digestive tract and rush
Into the double effects of growth.Probiotics preparation is with its unique mode of action, natural composition, it will be apparent that function and effect,
Become ideal Substitutes For Antibiotic.
Probiotics preparation refers in microecological balance theory, microbial state treatment theory, Tiny ecosystem nutrient theory and Tiny ecosystem
It prevents and treats under theoretical direction, by stringent breeding and domestication, is prepared by bioengineering and Tiny ecosystem engineering, animal body stomach can be passed through
The barriers such as acid, bile acid, pancreatic juice and survive, be finally colonized and breed in alimentary canal, adjust host in microbial state treatment, keep
Microecological balance, the probiotic and its metabolite and growth-promoting substance of raising host health level or the state that improves health
Product.
The microorganism that can be used as probiotics preparation must satisfy following several standards: (1) cannot generate any poison first
Element, i.e., it is nontoxic, harmless, safe without side-effects；(2) digestion of gastric acid, pepsin, trypsase and bile is shown
Certain resistance, so as to survive in alimentary canal；(3) it can be adhered to gastrointestinal mucosal, be permanently or temporarily colonized to property
In gastrointestinal tract；(4) colony balance or prevention ecological disturbance in host be may advantageously facilitate, have certain promotion to make host health
With；(5) there can be Clinical efficacy when its product is fed by Rational Dosage；(6) product is prepared and is able to maintain during storing higher
Active and longer stability；(7) it can survive in a variety of hosts.This last standard may be most difficult to reach, although
Most of species have microbiologic population in metastable enteron aisle, but each species have oneself unique bacterial community again.Moreover,
Some factors cause the kind for accounting for time dominant advantage if pressure or antibiotic treatment can change microbiota in enteron aisle
Group even pathogen becomes dominant advantage bacterium.Therefore, the adaptation of selection bacterial strain comprehensively considers, and selection as far as possible meets more above items
The bacterial strain of part.
Many probiotics are that discovery comes out from original enteric bacteria, and is proved have to some enterogastric diseases
Clinical efficacy.In addition to other than clinical effect is different with ingredient, their mechanism of effect of probiotics are different.Due to probiotics system
Agent can supplement the microflora lacked in enteron aisle in quantity or type, and Tiny ecosystem is flat in adjustable or maintenance enteron aisle
Weighing apparatus enhances immune function, promotes the digestion and absorption of nutriment, thus reach disease-free diseases prevention, it is ill to cure the disease, improve the conversion of word material
The effect of rate and livestock and poultry production performance.It is very big about the research report result difference of feed microbe additive so far, in theory
On also have various explanations.By the experience of research institute's accumulation to probiotics preparation in animal husbandry, now by probiotics preparation
Mechanism of action is summarized as follows:
1, maintain intestinal microecology balance: in animal gastrointestinal tract, there are a large amount of microbial floras, and keep relative equilibrium
Stable state.Some of them flora is of great significance to the growth and development of animal and resisting the disease.Under normal circumstances, animal intestine
Dominant population is anaerobic bacteria in road, accounts for 99% or more, including Bacillus acidi lactici, Bifidobacterium, digests bacillus and excellent bacillus etc.,
And aerobic bacteria and facultative anaerobic bacteria only account for 1%.These floras once lose balance, then the alimentary canal technical ability of animal occur disorderly
Disorderly, production performance decline, pathogenic microbes meeting mass propagation cause animal to fall ill, at this time can be effective using probiotics preparation
The quantity for supplementing beneficial bacterium, inhibits the growth and breeding of pathogenic flora, the colony balance of adjustment and recovery enteron aisle.Animal body maintains intestines
In terms of the mechanism of road microecological balance has following two: (1) colonization resistance: the ancestral home kind group energy of animal gastrointestinal tract inhibits outside other
Carry out microorganism to be colonized in enteron aisle, here it is colonization resistances, also cry competition exclution.(2) antibacterial substance: such as lactobacillus energy is generated
Generate bacteriocin, class bacteriocin and other metabolins with bacteriostasis.Bacteriocin is that bacterial metabolism synthesizes and divides in the process
Secrete the protide antibacterial substance that one kind in environment has inhibiting effect to the closer strain of affiliation.It is most known thin
Rhzomorph only has bacteriostasis to identical Gram-stained bacterium, but deeply with research, finds many bacteriocin sphere of actions
It is very wide.
2, biology takes oxygen by force: the beneficial aerobic bacteria in probiotics during growth and breeding, consumes in environment in animal intestinal tract
Oxygen, the concentration of local oxygen molecule can be reduced, keep enteron aisle micro- conducive to the field planting of normal flora obligate anaerobe and growth and breeding
The ecological balance restores normal, reaches disease preventing and treating purpose.Result of study shows that diarrhea piglet takes orally bacillus cereus for three days on end
Afterwards, Bifidobacterium, Bacillus acidi lactici dramatically increase in enteral anaerobic bacteria, and Escherichia coli, salmonella substantially reduce.Pig feeding ground
After clothing bacillus, anaerobic bacteria is increased in intestinal flora, and aerobic bacteria and facultative anaerobic bacteria especially Escherichia coli substantially reduce.
3, Ant agonism: the beneficial bacterium in probiotics preparation has antagonism to pathogenic microorganism in vivo, i.e., fixed
Plant drag.This colonization resistance is generated because of the adsorption site of internal microorganism and pathogenic bacteria competition gut epithelium.Such as
Occupied by more beneficial microbe, pathogenic microorganism will be ostracised these adsorption sites of fruit, and excrete with excrement.
Research report is pointed out extremely significant short of money with finding it after bacillus feeding chickling and having to chick enteral Escherichia coli and salmonella
Anti- effect.In vitro in test tube culture assays, lactobacillus probiotics have pathogenic S. pullonum very strong
Biological antagonist.
4, enhance the immune function of body: certain probiotics preparations are good immunoactivators, because itself is exactly
Nospecific immunity regulatory factor can stimulate body immune system to generate interferon, improve the concentration and macrophage of immunoglobulin
The activity of cell improves the humoral immunity of animal and cellular immune level, raising master of the probiotics preparation to animal immunizing power
Show the following aspects: (1) activity for enhancing macrophage has result of study to show that lactic acid bacteria can significantly increase macrophage
Cell activity.Cheese lactobacillus can improve macrophage to the phagocytic activity of Listeria；Separately some researches show that yeast extracts
It can reinforce the reaction of a variety of macrophages；(2) influence that horizontal, cell factor generates is generated to immune organ, antibody；(3) right
The activation and humidification of specific immune response.
5, digestive ferment is synthesized, promote the digestion and absorption of nutriment: the beneficial microbe in probiotics preparation can produce water
Enzyme, digestive ferment etc. are solved, is conducive to the complicated carbohydrate such as protein, the fat in degradation feed, improves food conversion ratio.
The Aspergillus such as aspergillus oryzae, aspergillus niger can generate cellulase, be conducive to digestion, absorption and utilization of the animal to cellulose.Black song
It is mould to generate amylase, protease, pectase.Certain saccharomycete play the role of Rich in Trace Element, are allowed to by inorganic states shape
Formula becomes the organic form of animal absorption easy to digest.Acid type probiotics feeding animals are produced with bacillus and Bacillus acidi lactici etc.
Afterwards, animal mucous membrane of small intestine gauffer increases, and continues Mao Jiachang, and mucous glands nest is deepened, and small intestine absorption area increases, to improve rate of body weight gain
With the utilization rate of feed.Bacillus subtilis and bacillus licheniformis, which remove, has stronger protease, amylase and lipase activity
Property outside, while the enzyme also with certain complex carbohydrates in degrading plant feed, such as pectase, dextranase, cellulose
Enzyme etc., wherein many enzymes are the enzymes that mammal and birds itself cannot generate.
6, improve intestinal environment, reduce the generation of the harmful substances such as ammonia, amine: the substances pair such as ammonia, amine, indoles, hydrogen sulfide
Animal intestinal tract mucomembranous cell has apparent toxic action.Lactic acid bacteria etc. can generate organic acid, bacteriocin etc. in enteron aisle growth and breeding
Inhibiting substances can inhibit the growth of the putrefactivebacterias such as enteral Escherichia coli, reduce the activity of urase, so reduce protein to
The conversion of the harmful substances such as ammonia, amine reduces the concentration of enteral ammonia, and reduces and outwardly drain, and improves the life cycle of livestock and poultry
Border.
Probiotics preparation is formally divided into probiotic, prebiotics and synbiotic in the world.Probiotic, that is, narrow sense probiotics
Preparation, refer to it is harmless to host, can play the role of improve the host intestine flora ecological balance active bacteria formulation and its metabolite.
There are lactic acid bacteria, Bifidobacterium, enterococcus, Escherichia coli, bacillus subtilis, lichens gemma applied to the probiotics of human body at present
Bacillus, bacillus cereus, clostridium butyricum and yeast etc..Prebiotics refer to cannot by Host Digestion absorb but can be selective
Ground promotes the substance of original one or more of beneficial bacterium growth and breedings in host intestine, by improving the quantity of beneficial bacterium, suppression
Harmful bacterial growth processed reaches intestinal microecology balance, promotes the purpose of body health to reach adjustment intestinal flora.Than
Such as bifidus factor, oligosaccharides substance or oligosaccharide etc..There are also certain Chinese herbal medicines in China, such as ginseng, Radix Codonopsis, Radix Astragali or tea
Leaf extract can also play the role of prebiotics.Synbiotic refers in preparation to include probiotic and the two-part system of prebiotics
Agent.This based article is theoretically the mixture for having both both above ingredient and characteristic, but this definition is seldom adopted.
Probiotics is generally segmented into three classes both at home and abroad, the first kind is the lactobacillus of general anaerobism, that is, oxytolerant, such as
Lactobacillus acidophilus, Lactobacillus casei etc.；Second class is the Bifidobacterium of strictly anaerobic, such as bifidobacterium longum, bifidobacterium breve
Deng；Third class is amphimicrobian coccus, such as enterococcus faecalis, newborn enterococcus.In addition, can be also included into there are also some saccharomycete with enzyme
The scope of probiotics.The probiotics being usually used in feed includes lactic acid bacteria, bacillus and saccharomycete etc..
Lactic acid bacteria be to a group energy using fermentable carbohydrate generate a large amount of lactic acid gram-positive bacterium it is logical
Claim.Lactic acid bacteria is defined as: cell be it is rod-shaped or spherical, Gram-positive does not generate peroxidating oxygenase, consumption of glucose
And 50% or more generates lactic acid, does not form endospore, without motion, or only minority has motility.It passes through life in animal body
Object antagonism reduces pH value, prevents and inhibit the harmful substances such as intrusion and field planting, degradation ammonia, indoles, the scatol of pathogenic bacteria, dimension
The normal ecological balance in enteron aisle is held, humoral immunity and cellular immunity are enhanced.Lactic acid bacteria can be used for lactation and weaning stage animal
In feed.Lactic acid bacteria is widely distributed in nature, not there is only in the enteron aisle of humans and animals and other organs, while there is also
In food, feed, soil, plant rhizosphere, river water lake.Lactic acid bacteria is in industry, agricultural, food and health care etc. and the mankind
There is very high application value in closely related field, can be used for probiotics preparation, lactic acid, silage inoculant bacteria, lactic fermentation
The production of food (such as fermented dairy product, fermented vegetable, fermentation meat product), bacteriocin, cosmetic humectant etc..Application at present
Lactic acid bacteria arise primarily at nearly 30 kinds of microorganism of Lactobacillus, nisin Pseudomonas and Bifidobacterium.China
The Ministry of Agriculture allows the lactic acid bacteria as feed addictive to have: Lactobacillus casei, lactobacillus plantarum, streptococcus fecalis, streptococcus faecalis, cream
7 kinds of sour piece coccus, lactobacillus acidophilus, streptococcus lactis etc..
Bacillus is the bacterium that passes by one's way of the enteron aisle for probiotic, cannot be colonized in enteron aisle.It is aerobic, harmless, can produce
It sprouts spore acid and alkali-resistance, high temperature resistant and extruding, with the stability of height in intestinal acidity environment, promotes the growth of beneficial bacterium,
Harmful bacteria in antagonism enteron aisle enhances immunity of organisms, improves disease resistance；More strongly active protease and amylase can be secreted,
The digestion that animal growth rate promotes feed nutrition substance can be significantly improved.Report that more strain has bacillus subtilis at present
The beneficial bacteriums types such as bacterium, bacillus licheniformis, bacillus cereus and Japan bacillus.
Saccharomycete for probiotics preparation is one is active dry yeasr preparation, and one is yeast cultures.Yeast cells is rich
Containing protein, nucleic acid, vitamin and a variety of enzymes, there is enhancing animal immunizing power, improve feed palatability, promote animal to feed
The functions such as abilities of digestive and absorption, and animal can be improved to the utilization rate of phosphorus；Its culture is full of nutrition, rich in B family vitamin,
Minerals, digestive ferment, somatomedin and more complete amino acid.The application age of yeast and its culture for a long time, extensively
In the general cultivation applied to various animals, the Ministry of Agriculture is allowed for the saccharomycete of probiotic to have saccharomyces cerevisiae, brewer's yeast at present
Deng.
Currently, lactobacillus paracasei, aspergillus oryzae and candida utili are compounded answering as pig nonreactive feed addictive
With there is not been reported.
Summary of the invention
Technical problem to be solved by the invention is to provide one kind can effectively improve diarrhea of pigs, and has no toxic side effect, no dirt
Dye, the pig having no drug resistance nonreactive feed.
The present invention solves the above problems the technical scheme adopted is that the nonreactive for providing a kind of microbial fermentation preparation is raised
Material, by batch be added composite bacteria liquid solid state fermentation be made, wherein composite bacteria liquid by lactobacillus paracasei bacterium solution 1-3 parts by weight,
Aspergillus oryzae bacterium solution 1-3 parts by weight and candida utili bacterium solution 1-3 parts by weight are mixed.
Preferably, the composite bacteria liquid by 2 parts by weight of lactobacillus paracasei bacterium solution, 1 parts by weight of aspergillus oryzae bacterium solution and produces protein
2 parts by weight of Candida bacterium solution compounding is made.
It is furthermore preferred that viable count is identical in three kinds of bacterium solutions of Unit Weight part.
Preferably, the batch includes dregs of beans, corn protein powder, rapeseed dregs, peanut meal, calcium monohydrogen phosphate and salt etc..
It is furthermore preferred that the batch further includes lysine, methionine and threonine etc..
It is furthermore preferred that the batch further includes vitamin A, vitamin C, vitamin E and vitamin B12Deng.
Most preferably, the batch by 50 parts by weight of dregs of beans, 20 parts by weight of corn protein powder, 10 parts by weight of rapeseed dregs,
5 parts by weight of peanut meal, 5 parts by weight of calcium monohydrogen phosphate, 5 parts by weight of salt, 1 parts by weight of lysine, 1 parts by weight of methionine, threonine 1
Parts by weight, 0.5 parts by weight of vitamin A, 0.5 parts by weight of vitamin C, 0.5 parts by weight of vitamin E and vitamin B120.5 weight
Amount part, which is uniformly mixed, to be made.
Preferably, the weight ratio of the batch and composite bacteria liquid is (90-95): (10-5).
It is furthermore preferred that the weight ratio of the batch and composite bacteria liquid is 95:5.
The invention has the advantages that:
It is surprising that by repeated tests, present inventors have unexpectedly found that by lactobacillus paracasei bacterium solution, aspergillus oryzae
The nonreactive feed obtained after bacterium solution and candida utili bacterium solution compounding through solid state fermentation has synergy for prevention and treatment diarrhea of pigs
Effect, cooperation other active components using effect is more preferably.
Compared with common pig's feed, nonreactive feed compatibility of the invention is reasonable, significant in efficacy, can significantly reduce pig abdomen
Rate is rushed down, experiments have shown that having special efficacy to diarrhea of pigs is improved；Due in feed without western medicine antibiotics ingredient, feed of the invention without
Toxic side effect has no drug resistance, pollution-free.
Specific embodiment
Below with reference to embodiment, the present invention will be further explained, and embodiments of the present invention are not limited thereto.Under
It states experimental method used in embodiment unless otherwise specified, is conventional method.
The preparation of embodiment 1, lactobacillus paracasei bacterium solution
Lactobacillus paracasei (bacterial strain GMNL-33) is seeded in the anaerobism pipe equipped with MRS culture medium, 37 DEG C of constant temperature are detested
Oxygen culture 48h, obtains lactobacillus paracasei bacterium solution.
The MRS culture medium are as follows: peptone 10.0g/L, beef extract 8.0g/L, yeast extract 4.0g/L, glucose 20.0g/
L, Tween 80 1ml/L, dipotassium hydrogen phosphate 2.0g/L, sodium acetate trihydrate 5.0g/L, Triammonium citrate 2.0g/L, epsom salt
0.2g/L, manganese sulfate 0.05g/L, pH 6.2-6.6.
The preparation of embodiment 2, aspergillus oryzae bacterium solution
Aspergillus oryzae (bacterial strain CICC40188) is seeded on Czapek ' s agar medium, 28 DEG C of culture 72h, then
It is inoculated into Czapek ' s fluid nutrient medium, 28 DEG C of constant temperature incubation 72h obtain aspergillus oryzae bacterium solution.
Czapek ' the s agar medium are as follows: sucrose 3%, sodium nitrate 0.3%, bitter salt 0.05%, chlorination
Potassium 0.05%, four ferrous sulfate hydrates 0.001%, dipotassium hydrogen phosphate 0.1%, agar 1.5%, pH value 6.0-6.5.Agar is not added
As Czapek ' s fluid nutrient medium.
The preparation of embodiment 3, candida utili bacterium solution
Candida utili (bacterial strain ATCC22023) is inoculated into wort agar medium, 28 DEG C of culture 72h,
It inoculates in malt juice liquid medium, 28 DEG C of constant temperature incubation 72h obtain candida utili bacterium solution.
The wort agar medium are as follows: brewer's wort is 5-6 Baume degrees, and pH value 6.5,2g/100ml agar, which is added, is
?.It is malt juice liquid medium that agar, which is not added,.
The preparation of embodiment 4, batch
Take 50 parts by weight of dregs of beans, 20 parts by weight of corn protein powder, 10 parts by weight of rapeseed dregs, 5 parts by weight of peanut meal, phosphoric acid hydrogen
5 parts by weight of calcium, 5 parts by weight of salt, 1 parts by weight of lysine, 1 parts by weight of methionine, 1 parts by weight of threonine, 0.5 weight of vitamin A
Measure part, 0.5 parts by weight of vitamin C, 0.5 parts by weight of vitamin E and vitamin B120.5 parts by weight are uniformly mixed to cooperate
Material.
The preparation (A1-A6) of embodiment 5, nonreactive feed
It is about 10 that the lactobacillus paracasei bacterium solution low temperature that embodiment 1 is prepared, which is concentrated into viable count in bacterium solution,8A/g,
It is added to according to 5% inoculum concentration in the batch that embodiment 4 is prepared, solid state fermentation 7d is under the conditions of 35 DEG C to get right
According to nonreactive feed A1.
It is about 10 that the aspergillus oryzae bacterium solution low temperature that embodiment 2 is prepared, which is concentrated into viable count in bacterium solution,8A/g, according to
5% inoculum concentration is added in the batch that embodiment 4 is prepared, and solid state fermentation 7d is under the conditions of 35 DEG C to get control nothing
Anti- feed A2.
It is about 10 that the candida utili bacterium solution low temperature that embodiment 3 is prepared, which is concentrated into viable count in bacterium solution,8A/g,
It is added to according to 5% inoculum concentration in the batch that embodiment 4 is prepared, solid state fermentation 7d is under the conditions of 35 DEG C to get right
According to nonreactive feed A3.
Lactobacillus paracasei bacterium solution, aspergillus oryzae bacterium solution and the candida utili bacterium solution that embodiment 1-3 is prepared are low
Temperature is concentrated into viable count in three kinds of bacterium solutions and is each about 108A/g, then composite bacteria liquid is mixed and made into the weight ratio of 2:2:1, according to
5% inoculum concentration is added in the batch that embodiment 4 is prepared, and solid state fermentation 7d is under the conditions of 35 DEG C to get the present invention
Nonreactive feed A4.
Lactobacillus paracasei bacterium solution, aspergillus oryzae bacterium solution and the candida utili bacterium solution that embodiment 1-3 is prepared are low
Temperature is concentrated into viable count in three kinds of bacterium solutions and is each about 108A/g, then composite bacteria liquid is mixed and made into the weight ratio of 1:2:2, according to
5% inoculum concentration is added in the batch that embodiment 4 is prepared, and solid state fermentation 7d is under the conditions of 35 DEG C to get the present invention
Nonreactive feed A5.
Lactobacillus paracasei bacterium solution, aspergillus oryzae bacterium solution and the candida utili bacterium solution that embodiment 1-3 is prepared are low
Temperature is concentrated into viable count in three kinds of bacterium solutions and is each about 108A/g, then composite bacteria liquid is mixed and made into the weight ratio of 2:1:2, according to
5% inoculum concentration is added in the batch that embodiment 4 is prepared, and solid state fermentation 7d is under the conditions of 35 DEG C to get the present invention
Nonreactive feed A6.
The application of test example 1, nonreactive feed of the present invention in prevention and treatment diarrhea of pigs
To verify application effect of the invention, feed addictive of the invention carries out in the porkling similar in health, weight
Test.Pig is divided into experimental group (A1-A6) and control group, every group is 50.Wherein, the daily ration of experimental group is the system of embodiment 5
Standby obtained nonreactive feed A1-A6, the daily ration of control group are the batch of identical weight prepared by embodiment 4, and each group pig is daily
It feeds 3 times, is freely eaten in experimental period, while sufficient drinking-water being provided, other every daily management measures are according to farm
Routine Management carries out, and periodically carries out immune and expelling parasite, and the Escherichia coli quantity in excrement, while statistical test phase are detected after 30 days
The incidence of pig in interior experimental group and control group calculates the diarrhea of pigs rate (being shown in Table 1) of each group.
Table 1
| Coliform count (107A/g) | Diarrhea rate | |
| Control group | 5.87 | 24% |
| Experimental group A1 | 3.16 | 16% |
| Experimental group A2 | 3.08 | 16% |
| Experimental group A3 | 3.59 | 18% |
| Experimental group A4 | 2.44 | 12% |
| Experimental group A5 | 2.13 | 10% |
| Experimental group A6 | 1.07 | 4% |
Nonreactive feed of the invention can significantly reduce large intestine bar in piglet body it can be seen from the comparative experiments of test example 1
Bacterium number amount reduces the chance of Infection in Piglets disease, reduces piglet disease incidence and diarrhea rate.During the test it has also been found that feeding this
The piglet state of mind of invention nonreactive feed is significantly better than control group, and honey stomach, the smooth color of fur is good.In addition, in bacterium solution
In the case that total amount remains unchanged, it is made of lactobacillus paracasei bacterium solution, aspergillus oryzae bacterium solution and candida utili bacterium solution compounding
Composite bacteria liquid made from the anti-diarrhea of pigs effect of nonreactive feed be substantially better than single bacterium solution, wherein lactobacillus paracasei bacterium solution,
Aspergillus oryzae bacterium solution and candida utili bacterium solution manufactured nonreactive feed prevention and treatment grice diarrhoea after being mixed with the weight ratio of 2:1:2
Effect is the most prominent, and grice diarrhoea rate is reduced to 4%, produces and is difficult to expected excellent effect.
Claims (3)
1. a kind of nonreactive feed of microbial fermentation preparation, is added composite bacteria liquid by batch and is made through solid state fermentation, feature
It is, wherein composite bacteria liquid is by 2 parts by weight of lactobacillus paracasei bacterium solution, 1 parts by weight of aspergillus oryzae bacterium solution and candida utili bacterium
2 parts by weight of liquid compounding be made, the batch by 50 parts by weight of dregs of beans, 20 parts by weight of corn protein powder, 10 parts by weight of rapeseed dregs,
5 parts by weight of peanut meal, 5 parts by weight of calcium monohydrogen phosphate, 5 parts by weight of salt, 1 parts by weight of lysine, 1 parts by weight of methionine, threonine 1
Parts by weight, 0.5 parts by weight of vitamin A, 0.5 weight of 0.5 parts by weight of vitamin C, 0.5 parts by weight of vitamin E and vitamin B12
Amount part, which is uniformly mixed, to be made.
2. nonreactive feed according to claim 1, which is characterized in that the weight ratio of the batch and composite bacteria liquid is
(90-95):(10-5)。
3. nonreactive feed according to claim 2, which is characterized in that the weight ratio of the batch and composite bacteria liquid is
95:5。
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| CN107312730B (en) * | 2017-07-11 | 2023-02-28 | 福建贝迪药业有限公司 | Compound Chinese herbal medicine microecological preparation and rapid production method thereof based on solid state fermentation |
| CN107254424B (en) * | 2017-07-11 | 2023-02-28 | 福建贝迪药业有限公司 | Novel liquid composite microecological preparation for livestock and poultry and preparation method thereof |
| CN107691849B (en) * | 2017-11-13 | 2021-06-29 | 播恩集团股份有限公司 | Feed prepared by microbial fermentation |
| CN107865232B (en) * | 2017-12-04 | 2021-06-29 | 河南正本清源科技发展股份有限公司 | Zymophyte liquid, product containing zymophyte liquid and used for improving intestinal health of pregnant sows, and preparation method and application of product |
| CN107897505B (en) * | 2017-12-04 | 2021-07-09 | 河南正本清源科技发展股份有限公司 | Zymophyte liquid, product containing zymophyte liquid and used for nursing pigs, and preparation method and application of product |
| CN111903848A (en) * | 2020-08-11 | 2020-11-10 | 张良 | Amino acid compound feed additive for animals and preparation method thereof |
| CN113430131B (en) * | 2021-06-18 | 2022-04-15 | 北京爱蓝生物科技有限公司 | Koji block for fermented feed and preparation method thereof |
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