CN104855674A - Production method for microbial fermentation complete feed by combining strain joint transformations - Google Patents
Production method for microbial fermentation complete feed by combining strain joint transformations Download PDFInfo
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- CN104855674A CN104855674A CN201510250641.7A CN201510250641A CN104855674A CN 104855674 A CN104855674 A CN 104855674A CN 201510250641 A CN201510250641 A CN 201510250641A CN 104855674 A CN104855674 A CN 104855674A
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Abstract
The invention discloses a production method for microbial fermentation complete feed by combining multi-strain joint transformations. The method for producing a microbial feed by utilizing needle mushroom residues, and performing mould and bacteria staged cultivation, and joint transformations and fermentation comprises the following process steps of processing raw materials, compounding a mould fermentation agent, preparing a composite bacterial fermentation agent, performing staged solid fermentation, drying, crushing and the like. The microbial feed prepared by the production method disclosed by the invention has natural fermentation fragrance without any chemical additive component and contains nutrient substances of various amino acids, vitamins and the like and a large number of bioactive enzymes, so that content of glutamic-pyruvic transaminase, glutamic oxalacetic transaminase, cholesterol, triglyceride and urea nitrogen in blood can be greatly reduced, the liver quality and the kidney quality of animals are improved, and the microbial fermentation complete feed is an environment-friendly biological feed with good quality and low price for feeding domestic animals.
Description
Technical field
The invention belongs to biological feedstuff fermentation production technology field, particularly a kind of fermentable complete feed production method combining multi-cultur es and combine conversion.
Background technology
Biological fermentation feed has achieved significant feeding effect in American-European and Japan since the seventies in last century, and the pig farm of current Denmark 60% uses fermented feed.Existing raising experiment shows: fermented feed can improve animal intestinal health, and develop immunitypty is improved food conversion ratio.
Along with the development of biotechnology and the universal of excellent species, and the external predigestion theory that Animal nutrition scholar proposed in recent years, some experts even predict that, biological fermentation feed replaces the main feed mode of present granule feed or mash feed by becoming, imply that huge commercial promise and economic interests.
At present, domestic and international fermented feed is mainly fermented bean dregs and the assorted dregs of rice of fermentation, and fermented bean dregs merchandise cost is higher, and the assorted dregs of rice of fermentation are usually by user's self manufacture, cannot ensure product quality.Needle mushroom slag produces the existing application of organic chemical fertilizer as fermenting raw materials, but there is not been reported to produce complete feed.
Summary of the invention
The present invention relates to one utilizes asparagus to gather in the crops rear mushroom slag, produce the method for Comprehesive biological feed by segmented microbial solid fermentation, comprise Feedstock treating, mold fermentation agent is composite, composite bacterial leavening makes, segmented solid fermentation, the processing step such as dry and pulverizing.
Mold fermentation agent bacterial classification composition and ratio are respectively:
Geotrichum candidum 20%-40%
Aspergillus oryzae 60%-80%
Composite bacterial leavening composition and volume ratio are respectively:
Lactobacillus fermenti 10%-50%
Lactobacillus rhamnosus 20%-40%
Saccharomycete 20%-40%
And each component spore count is respectively in above-mentioned mold fermentation agent:
Geotrichum candidum >=500,000,000/gram
Aspergillus oryzae >=500,000,000/gram
In composite bacterial leavening, each component viable count is respectively:
Lactobacillus fermenti >=3,000,000,000,000/ml
Lactobacillus rhamnosus >=20,000,000,000/ml
Saccharomycete >=2,000,000,000/ml
Further, each component optimum ratio of above-mentioned mold fermentation agent is geotrichum candidum 30%, aspergillus oryzae 70%, and in bacterial fermentation agent, each component optimum ratio is lactobacillus fermenti 45%, Lactobacillus rhamnosus 25%, saccharomycete 20%.
It is to be noted, the fermentation raw material adopted is the needle mushroom dreg containing wheat bran, corncob, cotton seed hulls and asparagus thalline, moisture is 35-45%, mold fermentation agent is seeded to bacterium slag by the 1%-5% of fermentate quality, mix, 30 ~ 35 DEG C of condition bottom fermentation pond aerlbic cultures 3 days, packed sealing, at 25 DEG C-35 DEG C condition bottom fermentation 5-7 days.
Advantage of the present invention is:
(1) edible fungi residue that this invention uses is the discarded object in the production of Edible Fungi enterprise product, secondary operations is carried out to it, a kind of Tiny ecosystem biological feedstuff of antibiotic-free of novel super quality and competitive price was both provided for animal husbandry and pig industry, has solved it and abandon the problem of environmental pollution burning and bring.
(2) product produced of this invention and microecological fermented forage not only can soften food fiber hardness, increase palatability, thus improve the utilization rate of feed significantly, Loss reducing, and containing a large amount of beneficial microbes (lactic acid bacteria can reach 50 ~ 10,000,000,000 CFU/g) in feed, and miscellaneous bacteria content is few.Be rich in the factor (lactic acid etc. as 2% ~ 3%) useful to animal growth.
(3) detect through experiment: by this invention mode of production domesticated animal, glutamic-pyruvic transaminase and glutamic-oxalacetic transaminease reflection liver in animal blood can be reduced and be subject to the undermined situations such as medicine inflammation; Urea nitrogen can reflect renal function, can reach protein metabolism and amino acid is in a basic balance; Cholesterol and triglycerides then reflect fat metabolism situation in animal body.The fermented feed that this invention obtains is fed market pig, glutamic-pyruvic transaminase in blood can be reduced significantly, glutamic-oxalacetic transaminease, cholesterol, triglycerides and urea nitrogen content, demonstrate the feature of market pig LFC, highlight this fermented feed and can accelerate vivotoxin metabolism, promote protein metabolism and amino acid balance, improve liver quality, improve renal function.
detailed description of the invention:
The present invention is described in further detail by following specific embodiment, but is not limited to the present embodiment.
Mold fermentation agent bacterial classification composition and ratio are respectively:
Geotrichum candidum 20%-40%
Aspergillus oryzae 60%-80%
Composite bacterial leavening composition and volume ratio are respectively:
Lactobacillus fermenti 10%-50%
Lactobacillus rhamnosus 20%-40%
Saccharomycete 20%-40%
And each component spore count is respectively in above-mentioned mold fermentation agent:
Geotrichum candidum >=500,000,000/gram
Aspergillus oryzae >=500,000,000/gram
In composite bacterial leavening, each component viable count is respectively:
Lactobacillus fermenti >=3,000,000,000,000/ml
Lactobacillus rhamnosus >=20,000,000,000/ml
Saccharomycete >=2,000,000,000/ml
Further, each component optimum ratio of above-mentioned mold fermentation agent is geotrichum candidum 30%, aspergillus oryzae 70%, and in bacterial fermentation agent, each component optimum ratio is lactobacillus fermenti 45%, Lactobacillus rhamnosus 25%, saccharomycete 20%.
Concrete implementation step is as follows:
(1) the mushroom slag after results asparagus being carried out drying, is 35-45% to moisture.
(2) by required geotrichum candidum, aspergillus oryzae bacterial classification respectively through inclined-plane cultivation, Shaking culture, kind song cultivate after, become solid fermentation agent in 20-40:60-80 ratio mixing manufacture.
(3) mould bacteria solid fermentation agent is seeded to needle mushroom slag in 1%-5% ratio, mixes, 30 ~ 35 DEG C of condition bottom fermentation pond aerlbic cultures 3 days.
(4) by required lactobacillus fermenti, bacillus coagulans and yeast seeds, respectively after Tube propagation, Shaking culture, seed tank culture, press the inoculum concentration of culture volume 1 ~ 5% again, cultivated bacterium liquid is accessed respectively fluid nutrient medium in fermentation tank to cultivate after 20 ~ 48 hours, become ripe bacterium liquid; By lactobacillus fermenti, bacillus coagulans and the ripe bacterium liquid of saccharomycete prepare leavening according to percent by volume 10 ~ 50: 20 ~ 40: 20 ~ 40.
(5) by the leavening of preparation, access fermentation vat according to the 0.1-0.5% of fermentate quality, mix, pack sealing, cultivation temperature 25 ~ 35 DEG C, cultivates 3 ~ 7 days, when the temperature of fermentate rises to 40 ~ 45 DEG C, and have larger sour-sweet aroma smell, material reaches 10 containing useful viable bacteria
8~ 10
12individual/g, fermentation ends;
(6) biological feedstuff fermented being dried to moisture is less than 12%, pulverizes, is fermentative feedstuff of microbe.
Below introduce several specific embodiment.
Embodiment 1:
(1) by geotrichum candidum 30%, aspergillus oryzae 70% obtains mold fermentation agent;
(2) mold fermentation agent is seeded to bacterium slag in 1% ratio of material quality, mixes, 30 ~ 35 DEG C of condition bottom fermentation pond aerlbic cultures 3 days;
(3) by lactobacillus fermenti 45%, Lactobacillus rhamnosus 25%, saccharomycete 20% obtains composite bacterial leavening;
(4) composite bacterial leavening is seeded to bacterium slag in material quality 0.1% ratio, packed sealing, at 25 DEG C-35 DEG C condition bottom fermentation 5-7 days.
Embodiment 2:
(1) by geotrichum candidum 25%, aspergillus oryzae 75% obtains mold fermentation agent;
(2) mold fermentation agent is seeded to bacterium slag in 1% ratio of material quality, mixes, 30 ~ 35 DEG C of condition bottom fermentation pond aerlbic cultures 3 days;
(3) by lactobacillus fermenti 40%, Lactobacillus rhamnosus 40%, saccharomycete 20% obtains composite bacterial leavening;
(4) composite bacterial leavening is seeded to bacterium slag in material quality 0.1% ratio, packed sealing, at 25 DEG C-35 DEG C condition bottom fermentation 5-7 days.
Embodiment 3:
(1) by geotrichum candidum 35%, aspergillus oryzae 65% obtains mold fermentation agent;
(2) mold fermentation agent is seeded to bacterium slag in 1% ratio of material quality, mixes, 30 ~ 35 DEG C of condition bottom fermentation pond aerlbic cultures 3 days;
(3) by lactobacillus fermenti 35%, Lactobacillus rhamnosus 40%, saccharomycete 25% obtains composite bacterial leavening;
(4) composite bacterial leavening is seeded to bacterium slag in material quality 0.1% ratio, packed sealing, at 25 DEG C-35 DEG C condition bottom fermentation 5-7 days.
Embodiment 4:
(1) by geotrichum candidum 40%, aspergillus oryzae 60% obtains mold fermentation agent;
(2) mold fermentation agent is seeded to bacterium slag in 1% ratio of material quality, mixes, 30 ~ 35 DEG C of condition bottom fermentation pond aerlbic cultures 3 days;
(3) by lactobacillus fermenti 40%, Lactobacillus rhamnosus 30%, saccharomycete 30% obtains composite bacterial leavening;
(4) composite bacterial leavening is seeded to bacterium slag in material quality 0.1% ratio, packed sealing, at 25 DEG C-35 DEG C condition bottom fermentation 5-7 days.
Contrast experiment's situation:
600 60 ages in days, growing and fattening pigs that body weight is close are divided into A, B two groups at random, often organize 300.A group is experimental group, and B group is control group, and two groups adopt the method for breeding of this invention to carry out unifying to raise by special messenger.A group adopts conventional base feed to add 10% fermented feed, and control group adopts conventional feed.After feeding 60 days, often organize and get 30 parts of pork samples at random and carry out blood hepatic and renal function mensuration.Admix the present invention in 10% ratio in A group daily ration and obtain microecological fermented forage.Experimental result is compared with control group, and the glutamic-pyruvic transaminase of A group reduces by 87.7%, and glutamic-oxalacetic transaminease reduces by 38.9%, and cholesterol reduces by 25%, and triglycerides reduces by 24.1%, and urea nitrogen reduces by 17.3%.A group Average weight increasing a day is 326 grams, and B group Average weight increasing a day is 267 grams, and experimental group is than control group many weightening finishes every day 59g, and daily gain increase rate reaches 22%, and difference is very remarkable.
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and description just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (5)
1. combine bacterial classification and combine the fermentable complete feed production method of conversion, comprise the processing steps such as Feedstock treating, mold fermentation agent are composite, the making of composite bacterial leavening, two-part solid fermentation, drying;
Mold species composition and volume ratio are respectively:
Geotrichum candidum 20%-40%
Aspergillus oryzae 60%-80%
Composite bacterial leavening composition and volume ratio are respectively:
Lactobacillus fermenti 10%-50%
Lactobacillus rhamnosus 20%-40%
Saccharomycete 20%-40%.
2. combination bacterial classification according to claim 1 combines the fermentable complete feed production method of conversion, it is characterized in that, in described mold fermentation agent, each component spore count is respectively:
Geotrichum candidum >=500,000,000/gram
Aspergillus oryzae >=500,000,000/gram
In composite bacterial leavening, each component viable count is respectively:
Lactobacillus fermenti >=3,000,000,000,000/ml
Lactobacillus rhamnosus >=20,000,000,000/ml
Saccharomycete >=2,000,000,000/ml.
3. combination bacterial classification according to claim 1 combines the fermentable complete feed production method of conversion, it is characterized in that, the each component optimum ratio of described mold fermentation agent is geotrichum candidum 30%, aspergillus oryzae 70%, in bacterial fermentation agent, each component optimum ratio is lactobacillus fermenti 45%, Lactobacillus rhamnosus 25%, saccharomycete 20%.
4. combination bacterial classification according to claim 1 combines the fermentable complete feed production method of conversion, it is characterized in that, described raw material is needle mushroom slag, and what remain after namely gathering in the crops asparagus includes but not limited to that wheat bran, corncob, cotton seed hulls and needle mushroom root divide.
5. combination bacterial classification according to claim 1 combines the fermentable complete feed production method of conversion, it is characterized in that, solid fermentation is with needle mushroom slag for substrate, and moisture is 35%-45%, and 1%-5% inoculation pressed by mold fermentation agent leavening by the first step, aerobic cultivation, 30 DEG C-35 DEG C condition bottom fermentations 3 days, 0.1%-0.5% inoculation is pressed in bacterial fermentation agent by second step, pack sealing, under 25 DEG C of-35 DEG C of conditions, through the anaerobic fermentation of 5-7 days.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105670967A (en) * | 2016-03-04 | 2016-06-15 | 朝阳市德凤生态养殖有限责任公司 | Microecological ferment for fattening pig feeds and preparation method thereof, and active fermentation feed |
| CN105994941A (en) * | 2016-05-24 | 2016-10-12 | 浙江伊杰斯生物科技有限公司 | Non-antibiotic feed prepared through microbial fermentation |
| CN107494986A (en) * | 2017-09-28 | 2017-12-22 | 合肥福泉现代农业科技有限公司 | A kind of preparation method for the compound microorganism bacterium powder that chicken feed is done for edible mushroom bran of fermenting |
| CN108486020A (en) * | 2018-05-16 | 2018-09-04 | 江南大学 | One plant of Lactobacillus rhamnosus and its application |
| JP2019013240A (en) * | 2015-08-31 | 2019-01-31 | セーホーエル．ハンセン アクティーゼルスカブ | Lactobacillus fermentum bacteria with antifungal activity |
| CN112042817A (en) * | 2020-09-27 | 2020-12-08 | 江苏省农业科学院 | Edible fungus fermentation fungus chaff with low mycotoxin content, and preparation method and application thereof |
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| CN102754744A (en) * | 2011-04-29 | 2012-10-31 | 弗曼燊生物科技(上海)有限公司 | Multi-bacteria fermented feed capable of improving pork tenderness |
| CN102754732A (en) * | 2011-04-29 | 2012-10-31 | 弗曼燊生物科技(上海)有限公司 | Microbial fermented feed production method adopting multi-fungus joint transformation |
| CN104293714A (en) * | 2014-10-08 | 2015-01-21 | 金年吉 | Bacterium generating agent and method for fermenting mushroom waste into feed by using bacterium generating agent |
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2015
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102754744A (en) * | 2011-04-29 | 2012-10-31 | 弗曼燊生物科技(上海)有限公司 | Multi-bacteria fermented feed capable of improving pork tenderness |
| CN102754732A (en) * | 2011-04-29 | 2012-10-31 | 弗曼燊生物科技(上海)有限公司 | Microbial fermented feed production method adopting multi-fungus joint transformation |
| CN104293714A (en) * | 2014-10-08 | 2015-01-21 | 金年吉 | Bacterium generating agent and method for fermenting mushroom waste into feed by using bacterium generating agent |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019013240A (en) * | 2015-08-31 | 2019-01-31 | セーホーエル．ハンセン アクティーゼルスカブ | Lactobacillus fermentum bacteria with antifungal activity |
| CN105670967A (en) * | 2016-03-04 | 2016-06-15 | 朝阳市德凤生态养殖有限责任公司 | Microecological ferment for fattening pig feeds and preparation method thereof, and active fermentation feed |
| CN105994941A (en) * | 2016-05-24 | 2016-10-12 | 浙江伊杰斯生物科技有限公司 | Non-antibiotic feed prepared through microbial fermentation |
| CN105994941B (en) * | 2016-05-24 | 2019-08-23 | 浙江伊杰斯生物科技有限公司 | A kind of nonreactive feed of microbial fermentation preparation |
| CN107494986A (en) * | 2017-09-28 | 2017-12-22 | 合肥福泉现代农业科技有限公司 | A kind of preparation method for the compound microorganism bacterium powder that chicken feed is done for edible mushroom bran of fermenting |
| CN108486020A (en) * | 2018-05-16 | 2018-09-04 | 江南大学 | One plant of Lactobacillus rhamnosus and its application |
| CN112042817A (en) * | 2020-09-27 | 2020-12-08 | 江苏省农业科学院 | Edible fungus fermentation fungus chaff with low mycotoxin content, and preparation method and application thereof |
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