CN102754732A - Microbial fermented feed production method adopting multi-fungus joint transformation - Google Patents
Microbial fermented feed production method adopting multi-fungus joint transformation Download PDFInfo
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- CN102754732A CN102754732A CN2011101095734A CN201110109573A CN102754732A CN 102754732 A CN102754732 A CN 102754732A CN 2011101095734 A CN2011101095734 A CN 2011101095734A CN 201110109573 A CN201110109573 A CN 201110109573A CN 102754732 A CN102754732 A CN 102754732A
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Abstract
The invention relates to a method for producing a microbial feed by utilizing flammulina velutipes residues through segmental cultivation and joint transformation and fermentation of funguses and bacteria. The method comprises the following process steps: raw material processing, fungus leavening agent formulation, compound bacteria leavening agent manufacturing, segmental solid fermentation, drying, crushing and the like. The microbial feed produced by adopting the method is free of any chemical additive ingredient, has a natural fermentation flavor, contains various amino acids, vitamins and other nutrient substances, as well as a great amount of biological active enzymes, and can remarkably reduce the contents of glutamic pyruvic transaminase, glutamic oxalacetic transaminase, cholesterol, triglyceride and urea nitrogen in blood and improve the quality of animal livers and kidneys, thereby being a super-quality and competitive-price green biological feed for feeding livestock.
Description
Invention field
The present invention relates to a kind of many bacterium associating microorganism transformed ferment complete feed production methods, belong to biological feedstuff fermenting and producing technical field.
Background of invention
Biological fermentation feed has been obtained significant feeding effect in American-European and Japan since the seventies in last century, fermented feed is used on the pig farm of Denmark 60% at present.Along with popularizing of development of biology and excellent species; And the external predigestion theory that proposed in recent years of Animal nutrition scholar; There is the expert to foretell; Biological fermentation feed will become the mode of mainly feeding that replaces present granule feed or mash feed, indicate huge commercial promise and economic interests.
At present, fermented feed is mainly the fermented bean dregs and the assorted dregs of rice of fermentation both at home and abroad, and the fermented bean dregs merchandise cost is higher, and the assorted dregs of rice of fermentation can't guarantee product quality usually by user's self manufacture.Asparagus mushroom slag is produced the existing application of organic chemical fertilizer as fermenting raw materials, does not appear in the newspapers as yet but produce complete feed.
Existing raising experiment shows: fermented feed can improve animal intestinal health, and enhance immunity power is improved food conversion ratio etc.But do not see the relevant report that biological fermentation feed improves the animal liver and kidney quality as yet.
Summary of the invention
The present invention relates to a kind of Asparagus results back mushroom slag that utilizes; Through the method that the segmented microbial solid fermentation is produced Comprehesive biological feed, comprise that raw material is handled, the mold fermentation agent is composite, processing steps such as the making of composite bacterial leavening, segmented solid fermentation, drying and pulverizing.
Mold fermentation agent bacterial classification is formed and ratio is respectively:
Trichoderma viride 20%-40%
Aspergillus niger 60%-80%
The composite bacterial leavening is formed and volume ratio is respectively:
Enterococcus faecalis 10%-50%
Bacillus coagulans 20%-40%
Saccharomycete 20%-40%
And each component spore count is respectively in the above-mentioned mold fermentation agent:
Trichoderma viride >=500,000,000/gram
Aspergillus niger >=500,000,000/gram
Each component viable count is respectively in the composite bacterial leavening:
Enterococcus faecalis >=3,000,000,000,000/ml
Bacillus coagulans >=20,000,000,000/ml
Saccharomycete >=2,000,000,000/ml
And each component optimum ratio of above-mentioned mold fermentation agent is a Trichoderma viride 30%, aspergillus niger 70%, and each component optimum ratio is an enterococcus faecalis 45% in the bacterial fermentation agent, bacillus coagulans 25%, saccharomycete 20%.
The present invention realizes through following steps:
(1) the mushroom slag that will gather in the crops after the Asparagus carries out drying, to moisture be 35-45%.
(2) with required Trichoderma viride, aspergillus niger strain respectively after the inclined-plane is cultivated, is shaken bottle and cultivates, plants bent the cultivation, by 20-40: the 60-80 mixed is made into the solid fermentation agent.
(3) mould bacteria solid fermentation agent is seeded to Asparagus mushroom slag in the 1%-5% ratio, mixes, 30～35 ℃ of condition bottom fermentation ponds ventilate and cultivated 3 days.
(4) with required enterococcus faecalis; Bacillus coagulans and saccharomycete bacterial classification; Respectively after bottle cultivation, seed tank culture are cultivated, shaken to test tube; Press the inoculum concentration of culture volume 1～5% again, after the bacterium liquid of being cultivated is inserted in the fermentation tank fluid nutrient medium respectively and cultivates 20～48 hours, become ripe bacterium liquid; With enterococcus faecalis, the ripe bacterium liquid of bacillus coagulans and saccharomycete is according to percent by volume 10～50: 20～40: 20～40 preparation leavenings.
(5) with the leavening of preparation, the 0.1-0.5% access fermentation vat according to the fermentate quality mixes; The pack sealing, 25～35 ℃ of cultivation temperature were cultivated 3～7 days; When the temperature of fermentate rises to 40～45 ℃, and bigger sour-sweet aroma smell is arranged, material contains useful viable bacteria and reaches 10
8～10
12Individual/g, fermentation ends;
(6) biological feedstuff that ferments being dried to moisture is below 12%, pulverizes, and is fermentative feedstuff of microbe.
Advantage of the present invention is:
(1) edible fungi residue of this invention use is reprocessed it for industrial agricultural production discarded object, and the biological feedstuff of novel super quality and competitive price both is provided for animal husbandry, has solved it again and has abandoned the problem of environmental pollution that burning brings.
(2) glutamic-pyruvic transaminase and glutamic-oxalacetic transaminease reflection liver receives damages such as medicine inflammation in the animal blood, urea nitrogen reflection renal function, protein metabolism and amino acid balance, and cholesterol and triglycerides then reflect fat metabolism situation in the animal body.The fermented feed that this invention the obtains market pig of feeding can reduce glutamic-pyruvic transaminase in the blood, glutamic-oxalacetic transaminease significantly; Cholesterol, triglycerides and urea nitrogen content demonstrate the characteristic of pig LFC; Highlight this fermented feed and can accelerate the vivotoxin metabolism; Promote protein metabolism and amino acid balance, improve the liver quality, improve renal function.
The specific embodiment:
The present invention further details through following specific embodiment, but is not limited to present embodiment.
Mold fermentation agent bacterial classification is formed and ratio is respectively:
Trichoderma viride 20%-40%
Aspergillus niger 60%-80%
The composite bacterial leavening is formed and volume ratio is respectively:
Enterococcus faecalis 10%-50%
Bacillus coagulans 20%-40%
Saccharomycete 20%-40%
And each component spore count is respectively in the above-mentioned mold fermentation agent:
Trichoderma viride >=500,000,000/gram
Aspergillus niger >=500,000,000/gram
Each component viable count is respectively in the composite bacterial leavening:
Enterococcus faecalis >=3,000,000,000,000/ml
Bacillus coagulans >=20,000,000,000/ml
Saccharomycete >=2,000,000,000/ml
And each component optimum ratio of above-mentioned mold fermentation agent is a Trichoderma viride 30%, aspergillus niger 70%, and each component optimum ratio is an enterococcus faecalis 45% in the bacterial fermentation agent, bacillus coagulans 25%, saccharomycete 20%.
The fermentation raw material that adopts is the golden mushroom slag that contains wheat bran, corncob, cotton seed hulls and Asparagus thalline; Moisture is 35-45%; The mold fermentation agent is seeded to the bacterium slag by the 1%-5% of fermentate quality, mixes, and 30～35 ℃ of condition bottom fermentation ponds ventilate and cultivated 3 days; Packed sealing was 25 ℃ of-35 ℃ of condition bottom fermentations 5-7 days.
Embodiment 1:
(1) press Trichoderma viride 30%, aspergillus niger 70% makes the mold fermentation agent
(2) the mold fermentation agent is seeded to the bacterium slag in 1% ratio of raw materials quality, mixes, 30～35 ℃ of condition bottom fermentation ponds ventilate and cultivated 3 days
(3) press enterococcus faecalis 45%, bacillus coagulans 25%, saccharomycete 20% makes the composite bacterial leavening
(4) the composite bacterial leavening is seeded to the bacterium slag in raw materials quality 0.1% ratio, packed sealing was 25 ℃ of-35 ℃ of condition bottom fermentations 5-7 days.
Embodiment 2:
(1) press Trichoderma viride 25%, aspergillus niger 75% makes the mold fermentation agent
(2) the mold fermentation agent is seeded to the bacterium slag in 1% ratio of raw materials quality, mixes, 30～35 ℃ of condition bottom fermentation ponds ventilate and cultivated 3 days
(3) press enterococcus faecalis 40%, bacillus coagulans 40%, saccharomycete 20% makes the composite bacterial leavening
(4) the composite bacterial leavening is seeded to the bacterium slag in raw materials quality 0.1% ratio, packed sealing was 25 ℃ of-35 ℃ of condition bottom fermentations 5-7 days.
Embodiment 3:
(1) press Trichoderma viride 35%, aspergillus niger 65% makes the mold fermentation agent
(2) the mold fermentation agent is seeded to the bacterium slag in 1% ratio of raw materials quality, mixes, 30～35 ℃ of condition bottom fermentation ponds ventilate and cultivated 3 days
(3) press enterococcus faecalis 35%, bacillus coagulans 40%, saccharomycete 25% makes the composite bacterial leavening
(4) the composite bacterial leavening is seeded to the bacterium slag in raw materials quality 0.1% ratio, packed sealing was 25 ℃ of-35 ℃ of condition bottom fermentations 5-7 days.
Embodiment 4:
(1) press Trichoderma viride 40%, aspergillus niger 60% makes the mold fermentation agent
(2) the mold fermentation agent is seeded to the bacterium slag in 1% ratio of raw materials quality, mixes, 30～35 ℃ of condition bottom fermentation ponds ventilate and cultivated 3 days
(3) press enterococcus faecalis 40%, bacillus coagulans 30%, saccharomycete 30% makes the composite bacterial leavening
(4) the composite bacterial leavening is seeded to the bacterium slag in raw materials quality 0.1% ratio, packed sealing was 25 ℃ of-35 ℃ of condition bottom fermentations 5-7 days.
Contrast experiment's situation:
400 60 ages in days, growing and fattening pigs that body weight is close are divided into two groups of A, B.The A group is experimental group, and the B group is control group, 200 every group, after feeding 60 days, gets 10 parts of pork samples at random for every group and carries out blood hepatic and renal function mensuration.Admix the present invention in 10% ratio in the A group daily ration and obtain fermentative feedstuff of microbe.Experimental result is to compare with control group, and the glutamic-pyruvic transaminase of A group reduces by 87.7%, and glutamic-oxalacetic transaminease reduces by 38.9%, and cholesterol reduces by 25%, and triglycerides reduces by 24.1%, and urea nitrogen reduces by 17.3%.
Claims (5)
1. the associating of bacterium more than kind microorganism transformed fermented feed production method comprises that raw material is handled, processing steps such as the mold fermentation agent is composite, the making of composite bacterial leavening, two-part solid fermentation, drying.
The mould bacterial classification is formed and volume ratio is respectively:
Trichoderma viride 20%-40%
Aspergillus niger 60%-80%
The composite bacterial leavening is formed and volume ratio is respectively:
Enterococcus faecalis 10%-50%
Bacillus coagulans 20%-40%
Saccharomycete 20%-40%.
2. method according to claim 1 is characterized in that each component spore count is respectively in the said mold fermentation agent:
Trichoderma viride >=500,000,000/gram
Aspergillus niger >=500,000,000/gram
Each component viable count is respectively in the composite bacterial leavening:
Enterococcus faecalis >=3,000,000,000,000/ml
Bacillus coagulans >=20,000,000,000/ml
Saccharomycete >=2,000,000,000/ml.
3. a kind of many bacterium associating microorganism transformed fermented feed production methods according to claim 1; It is characterized in that each component optimum ratio of said mold fermentation agent is a Trichoderma viride 30%; Aspergillus niger 70%; Each component optimum ratio is an enterococcus faecalis 45% in the bacterial fermentation agent, bacillus coagulans 25%, saccharomycete 20%.
4. a kind of many bacterium associating microorganism transformed fermented feed production methods according to claim 1; It is characterized in that said raw material is an Asparagus mushroom slag, promptly gather in the crops residual wheat bran, corncob, cotton seed hulls and the Asparagus mushroom root portion of including but not limited to after the Asparagus.
5. a kind of many bacterium associating microorganism transformed fermented feed production methods according to claim 1 is characterized in that solid fermentation is is substrate with Asparagus mushroom slag, and moisture is 35%-45%; The first step is pressed 1%-5% inoculation with mold fermentation agent leavening, aerobic cultivation, 30 ℃ of-35 ℃ of condition bottom fermentations 3 days; Second step was pressed the 0.1%-0.5% inoculation with the bacterial fermentation agent; The pack sealing, under 25 ℃ of-35 ℃ of conditions, the anaerobic fermentation through 5-7 days.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102987140A (en) * | 2012-11-28 | 2013-03-27 | 张有聪 | Method for preparing novel ruminant feed by using beneficial bacteria and plant fiber |
| CN103053858A (en) * | 2013-01-31 | 2013-04-24 | 广东省农业科学院蚕业与农产品加工研究所 | Flammulina velutipes fungus chaff feed and production method and application thereof |
| CN103120274A (en) * | 2013-03-09 | 2013-05-29 | 湖北富士峰生物科技有限公司 | Silage prepared by utilizing needle mushroom dreg and processing method thereof |
| CN103461673A (en) * | 2013-09-18 | 2013-12-25 | 肇庆长龙生物科技有限公司 | Method for preparing mycoprotein by taking glycerin residue as raw material |
| CN104855674A (en) * | 2015-05-18 | 2015-08-26 | 上海佳俊生物科技有限公司 | Production method for microbial fermentation complete feed by combining strain joint transformations |
| CN104996722A (en) * | 2015-08-13 | 2015-10-28 | 江南大学 | Method of two-step united multi-strain fermented feed |
| CN105010731A (en) * | 2015-05-12 | 2015-11-04 | 河南科技大学 | Plant protein mixture preparation method |
| CN107156444A (en) * | 2017-05-03 | 2017-09-15 | 北京农业职业学院 | Pleurotus eryngii mushroom bran and uncommercialized mushroom body feedstuff and its preparation method and application |
-
2011
- 2011-04-29 CN CN2011101095734A patent/CN102754732A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102987140A (en) * | 2012-11-28 | 2013-03-27 | 张有聪 | Method for preparing novel ruminant feed by using beneficial bacteria and plant fiber |
| CN102987140B (en) * | 2012-11-28 | 2014-07-09 | 张有聪 | Method for preparing novel ruminant feed by using beneficial bacteria and plant fiber |
| CN103053858A (en) * | 2013-01-31 | 2013-04-24 | 广东省农业科学院蚕业与农产品加工研究所 | Flammulina velutipes fungus chaff feed and production method and application thereof |
| CN103053858B (en) * | 2013-01-31 | 2014-08-06 | 广东省农业科学院蚕业与农产品加工研究所 | Flammulina velutipes fungus chaff feed and production method and application thereof |
| CN103120274A (en) * | 2013-03-09 | 2013-05-29 | 湖北富士峰生物科技有限公司 | Silage prepared by utilizing needle mushroom dreg and processing method thereof |
| CN103461673A (en) * | 2013-09-18 | 2013-12-25 | 肇庆长龙生物科技有限公司 | Method for preparing mycoprotein by taking glycerin residue as raw material |
| CN105010731A (en) * | 2015-05-12 | 2015-11-04 | 河南科技大学 | Plant protein mixture preparation method |
| CN104855674A (en) * | 2015-05-18 | 2015-08-26 | 上海佳俊生物科技有限公司 | Production method for microbial fermentation complete feed by combining strain joint transformations |
| CN104996722A (en) * | 2015-08-13 | 2015-10-28 | 江南大学 | Method of two-step united multi-strain fermented feed |
| CN104996722B (en) * | 2015-08-13 | 2018-04-06 | 江南大学 | A kind of method of the step combined ferment feed of multi-cultur es two |
| CN107156444A (en) * | 2017-05-03 | 2017-09-15 | 北京农业职业学院 | Pleurotus eryngii mushroom bran and uncommercialized mushroom body feedstuff and its preparation method and application |
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Application publication date: 20121031 |