CN105707434A - Method and application of fermented biological feed prepared by multi-strain mixed fermentation - Google Patents
Method and application of fermented biological feed prepared by multi-strain mixed fermentation Download PDFInfo
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Abstract
The present invention discloses a method and an application of a fermented biological feed prepared by multi-strain mixed fermentation. The fermented biological feed high in acid production and rich in organic acids, enzymes and probiotics is obtained by screening out 4 kinds of strains, and using two times of aerobic fermentation and 1 time of anaerobic fermentation. The obtained fermented biological feed is also rich in unsaturated fatty acids and aromatic acids, can stimulate the appetite of animals, has relatively good palatability, can effectively reduce trophism and bacterium caused diarrhea, dysentery and other intestinal problems in livestock and poultry, reduces ammonium odour in breeding environmental, purifies feeding environment, improve body immunity of animals, ensures animal health, and can completely or partially replace attractant agents, acidifying agents, enzyme preparations, probioties, anti-mildew agents and antibiotics. Pig feed with the addition of the fermented biological feed prepared by multi-strain mixed fermentation can increase the daily weight gain by 6% or above, can significantly improve the feed intake of the animals and the feed conversion rate, and can decrease the feed conversion ratio by 4% or above.
Description
Technical field
The present invention relates to fermenting organism field of feed, prepare the methods and applications of fermenting organism feedstuff more particularly, to a kind of multiple bacteria compound fermentation.
Background technology
Fermentative feedstuff of microbe broadly refers to use the composition not easily absorbed by animal in microbial decomposition feedstuff, raw material or mineral, makes feedstuff or feed preparations be more easy to by animal digestibility and utilization.In recent years, along with many countries limit and prohibit the use of antibiotic in the world, and feed resource day is becoming tight, and searching is efficient, environmental health type feedstuff becomes the task of top priority, and this provides wide development space for fermentative feedstuff of microbe.
Manioc waste is exactly the leftover bits and pieces after Maninot esculenta crantz. extracts starch, and key component is the crust of Maninot esculenta crantz. and internal parenchyma, and national annual manioc waste yield is up to millions of tons.Both at home and abroad the research of manioc waste is concentrated mainly on for producing single cell protein, ethanol and cultivation mushroom etc. at present, but industrial consumption is only small, substantial amounts of manioc waste is dropped, and causes great waste on the one hand, and manioc waste discarded on the other hand also pollutes welding.Along with being stepped up of cassava production, the recycling of manioc waste more seems significant.
The present invention utilizes multiple bacteria compound fermentation technology, by carbohydrate-modifying generation organic acid such as the starch remained in manioc waste, celluloses, prepares the fermenting organism feedstuff rich in organic acid, digestive enzyme and probiotic bacteria.Not only solve the environmental pollution that manioc waste causes, it is also possible to improve the palatability of manioc waste, improve feed intake and the feed conversion rate of animal；Obtained multiple bacteria compound fermentation biological feedstuff is also rich in multiple unsaturated fatty acid and aromatic acid, can stimulating animal appetite, there is good attractant, effectively reduce the bowel problems such as trophism and bacillary caused livestock and poultry diarrhea, dysentery, the ammonia of minimizing breeding environment is smelly, purify feeding environment, improve animal body immunity, ensure animal health, it can substitute phagostimulant, acidulant, enzyme preparation, microbial ecological agent and antifungus agent, antibiotic wholly or in part, reduces the environmental pollution that antibiotic brings.
Summary of the invention
A kind of multiple bacteria compound fermentation provided by the present invention, converts the preparation method producing manioc waste fermenting organism feedstuff.
Second purpose of the present invention is to provide the manioc waste fermenting organism feedstuff that said method obtains.
3rd purpose of the present invention is to provide the application of above-mentioned fermenting organism feedstuff.
It is an object of the invention to be achieved by the following technical programs:
A kind of multiple bacteria compound fermentation prepares the method for fermenting organism feedstuff, it is characterised in that comprise the following steps:
S1. first time aerobic fermentation: obtain compound after manioc waste, wheat bran, bean cake, aspergillus niger and yeast being mixed, 28～30 DEG C of fermentation 4～5d；
S2. second time aerobic fermentation: add lactic acid bacteria and continue fermentation 1d；
S3. anaerobic fermentation: add wheat bran, TONGKANG, and add bacillus subtilis and lactic acid bacteria, be distributed in bag by the material unstrained spirits of mix homogeneously, after unlimited bag mouth places a period of time under room temperature, is evacuated and hermetically sealed bag mouth, continues heap fermentation 2～3 weeks under room temperature；
The source of described aspergillus niger is ACCC30162, and the source of yeast is BNCC336054, and the source of bacillus subtilis is ACCC10746, and the source of lactic acid bacteria is CICC20680.
The present invention is mainly through filtering out this 4 kinds of bacterial strains, and by the interaction of these 4 kinds of bacterial strains, twice aerobic fermentation and an anaerobic fermentation is utilized to obtain and produce the fermentation material that acid amount is higher, under ensureing the premise producing acid amount, substantial amounts of probiotic bacteria can also be obtained, and producing enough enzymes, the content of the bacterium of fermentation material biological feedstuff, enzyme and the acid that finally obtain is all significantly high, can significantly improve feed intake and the feed conversion rate of animal.
Preferably, in described compound, the mass fraction of manioc waste is 40～60%, and wheat bran is 1～2%, and bean cake is 1～4.5%, aspergillus niger 0.04～0.08%, and inoculum of dry yeast is 105～106Individual/g compound, surplus is water.
Inoculum concentration is notable for the final influential effect that ferments, it is preferable that the inoculum concentration of lactic acid bacteria described in S2 is 106～107Individual/g compound；The inoculum concentration of bacillus subtilis described in S3 and lactic acid bacteria is 10 respectively6～107Individual/g compound and 106Individual/g compound.
Anaerobic fermentation is the Main Stage producing acid, and after optimization, the moisture of the material unstrained spirits of mix homogeneously described in S3 is 45%；S3 at room temperature opens wide bag mouth to place 7～8h, makes material unstrained spirits water content in bag be down to 25%.
The present invention also provides for the multiple bacteria compound fermentation biological feedstuff that said method obtains.
Utilizing multiple bacteria compound fermentation biological feedstuff of the present invention, mix with live pig solar eclipse feedstuff, to piglets, the sow of middle pig, big pig and suckling has significant beneficial effect.
To piglets, middle pig and big pig, containing a large amount of probioticss in fermenting organism feedstuff, enter in piglets body with pathogen contention nutrient attachment area；Organic acid in fermenting organism feedstuff can effectively suppress harmful microbe growth in piglets body, and in feces, the content of harmful microorganism, ammonia, hydrogen sulfide and scatol substantially reduces；All these are all conducive to building the good gastrolintestinal microecology environment of piglets and pig house environment, reduce the generation of gastrointestinal and respiratory tract disease.
For nursing sow, this fermenting organism feedstuff can improve nursing sow feed intake, increases lactation amount, improves milk water quality.Hot summer, when nursing sow feed intake is generally reduced, this effect is significant；Also can improve nursing sow health level, reduce mastitis, metritic generation；Postweaning estrus, breeding are normally.
Therefore, the present invention also provides for the application in promoting pig growth and development of the described multiple bacteria compound fermentation biological feedstuff.
The present invention also provides for the application in promoting milking sow lactation amount, improving milk water quality of the described multiple bacteria compound fermentation biological feedstuff.
Above-mentioned application is described multiple bacteria compound fermentation biological feedstuff and live pig solar eclipse feedstuff are mixed immediately feeding；If plant's autogamy, it is possible to add described multiple bacteria compound fermentation biological feedstuff 20～40kg in powder per ton；If feed factory is granulated, multiple bacteria compound fermentation biological feedstuff 40kg can be added in particulate material per ton.
Compared with prior art, the method have the advantages that
The preparation method that the invention provides a kind of multiple bacteria compound fermentation biological feedstuff, mainly through filtering out 4 kinds of bacterial strains, and by the interaction of these 4 kinds of bacterial strains, twice aerobic fermentation and an anaerobic fermentation is utilized to obtain and produce the fermentation material that acid amount is higher, under ensureing the premise producing acid amount, moreover it is possible to obtain substantial amounts of bacterium, and produce enough enzymes, the content of the bacterium of the fermented feed finally obtained, enzyme and acid is all significantly high, can significantly improve feed intake and the feed conversion rate of animal；Obtained multiple bacteria compound fermentation feedstuff is rich in multiple unsaturated fatty acid and aromatic acid, can stimulating animal appetite, there is good attractant, effectively reduce trophism and bacillary caused livestock and poultry diarrhea, the bowel problems such as dysentery, reduce ammonia smelly, purify feeding environment, improve animal body immunity, ensure animal health, it can substitute phagostimulant completely, acidulant, enzyme preparation, microbial ecological agent and antifungus agent, part antibiotic, after pig feed adds described multiple bacteria compound fermentation biological feedstuff, daily gain can improve more than 6%, feedstuff-meat ratio can reduce by more than 4%.
Accompanying drawing explanation
Fig. 1 is the process chart of method described in embodiment 1；Described leaven I is aspergillus niger and yeast, and described leaven II is lactic acid bacteria.
Detailed description of the invention
Further illustrate present disclosure below in conjunction with Figure of description and specific embodiment, but should not be construed as limitation of the present invention.Without departing from the spirit and substance of the case in the present invention, the simple modification that the inventive method, step or condition are made or replacement, belong to the scope of the present invention；If not specializing, the conventional means that technological means used in embodiment is well known to those skilled in the art.
In detailed description of the invention of the present invention, the source of described aspergillus niger is ACCC30162, and the source of yeast is BNCC336054, and the source of bacillus subtilis is ACCC10746, and the source of lactic acid bacteria is CICC20680.
The preparation of embodiment 1 multiple bacteria compound fermentation biological feedstuff
The preparation method of multiple bacteria compound fermentation biological feedstuff, comprises the following steps:
(1) first time aerobic fermentation:
(1) aspergillus niger and yeast activation: by raw material per ton add 4/10000ths aspergillus niger and yeast 50～100g(by yeast content 20,000,000,000/g), quantitatively weigh aspergillus niger and yeast mycopowder, join 5L 1% molasses water in (institute's water consumption will count among total amount of water), under 25 DEG C～28 DEG C conditions, activate 1～2 hour.
(2) raw material mixing: according to expection inventory, after manioc waste, wheat bran, bean cake quantitatively being weighed, premixing in stirring mixer (consumption is table 1 such as), is subsequently adding aspergillus niger and yeast activating solution is sufficiently mixed；Stir, mixed fermentation material is transferred to the interior cultivation fermentation of fermentation bed (pond).
Mixed fermentation material enters fermentation bed or fermentation vat, and material unstrained spirits ulking thickness is advisable with 25cm～30cm, and the microorganism in material unstrained spirits starts growth and breeding.In the 2nd day～the 4th day course of cultivating the microorganism, in material unstrained spirits, growth of microorganism is rapid, and quantity of heat production is big, should observe material unstrained spirits variations in temperature at any time, notes timely stirring, aeration-cooling.When temperature is more than 32 DEG C, but during lower than 35 DEG C, start the cooling of upender stirring；When temperature is more than 35 DEG C, should stirring stronger ventilation amount, promotion material unstrained spirits cooling immediately.When substantially mycelia (hold in the palm in bulk) visible in material unstrained spirits, but temperature less than 32 DEG C time, it is necessary to carry out stirring and interrupt mycelia, promote mycelial growth；First time aerobic fermentation continues 4 days～5 days altogether.
(2) second time aerobic fermentation
After first time aerobic fermentation, by lactic acid bacteria amount 106～107The addition of individual/g adds lactic acid bacteria in material unstrained spirits.Operational approach: quantitatively weigh lactic acid bacteria mycopowder (addition per ton is 500g～1kg(content of lactic acid bacteria 100,000,000,000/g)), under molasses water (molasses water consumption presses 0.5kg/ ton) room temperature with 1% after (25 DEG C～30 DEG C) activation 1h, in conjunction with in stirring even spraying to material unstrained spirits, continue cultivation and fermentation 1 day.
(3) anaerobic fermentation
(1) activation of leaven: by the addition (second time aerobic fermentation material unstrained spirits total amount) of 10～50g/ ton, (bacillus subtilis bacterial content is more than 10 to weigh bacillus subtilis mycopowder11Individual/g), weigh lactic acid bacteria mycopowder (content of lactic acid bacteria 100,000,000,000/g) by the addition of 10～50g/ ton, bacillus subtilis mycopowder and lactic acid bacteria mycopowder are placed in 1% molasses water of 5L and activate 1h(activate with reference to above-mentioned yeast).
(2) new raw material is added: the material unstrained spirits after second time aerobic fermentation being completed holds together heap and loads mixer.It is separately added into wheat bran and TONGKANG, premixing again by 15% and the 20% of material unstrained spirits total amount.By the bacillus subtilis after activation and in the water that is dissolved in of lactic acid bacteria mixed liquor (water yield be two-stage material unstrained spirits total amount 1%), after stirring, be sufficiently mixed with the fermentation material unstrained spirits after premix；Mixed material unstrained spirits water content control is 45%.
(3) by mixing after and water content 45% material unstrained spirits be dispensed in plastic bag, be placed in room temperature (25 DEG C～30 DEG C) lower open mouth cultivation 7～8 hours, treat that moisture is down to about 25%, cultivation terminates.Then with vacuum pump, the air in packaging bag is extracted out, and close charging inner bag bag mouth immediately, under room temperature, continue heap fermentation 2～3 weeks.
After anaerobic fermentation is terminated, obtain fermenting organism feedstuff, carry out visual examination and sampling Detection (table 2).
Comparative example 1
Experimental technique is with embodiment 1, and uniquely the difference is that the aspergillus niger CICC2108 selected, its bacteria containing amount 0.04%, originating in lactic acid bacterium CICC23609, bacteria containing amount is 108Individual/g, bacillus subtilis source is CICC10732, and bacteria containing amount is 108, yeast sources is CICC1063, and bacteria containing amount is 107Individual/g.To the final fermenting organism feedstuff obtained detection, its acid content is 120g/kg.
Comparative example 2
Experimental technique with embodiment 1, uniquely the difference is that in anaerobic fermentation control (2) mixed material unstrained spirits water content be 70%.To the final fermenting organism feedstuff obtained detection, its acid content is 150g/kg.
Embodiment 2 piglets stage result of use
By adding the fermenting organism feedstuff that a certain amount of embodiment 1 prepares in feedstuff, observe the impact on piglets growth and production effect.
DLY lean meat species health meat piglets 90, parity, age in days, sex, body weight are close, are randomly divided into 6 hurdles, 15, every hurdle, and two hurdles are one group, totally three groups: matched group, test one group and experiment two groups.
Matched group: this autogamy piglets powder
Test one group: matched group formula+2% fermenting organism feedstuff, powder
Test two groups: matched group formula+4% fermenting organism feedstuff, powder
Feed mode: free choice feeding, is advisable with slightly surplus material in morning next day, it is ensured that sufficient clean drinking-water.
Observed content: the mental status, fecaluria situation, coat condition etc..
Data record: test head number, the every daily material consumption of pig, average daily gain, feedstuff-meat ratio etc..
By the mental status, fecaluria situation, coat condition etc. are contrasted: experimental group each side is superior to matched group, the fermenting organism feedstuff (testing two groups) wherein adding 4% shows the fermenting organism feedstuff being better than adding 2%, mental act is more vivaciously active, feces does not digest niblet fewer, basic odorless.Testing two groups and have Pi Hong, the effect that hair is bright, table 3 is for searching for food, and increase weight correction data.
Rich in multiple organic acid in the fermenting organism feedstuff that embodiment 1 prepares, make feedstuff have a kind of special fermentation fragrance tart flavour, can the appetite of obvious stimulation animal, improve little pig feed intake.Test one group and improve 1.24% than matched group, test two groups preferably, higher by 3.73% than testing one group；
It addition, containing a large amount of unknown somatomedins in fermenting organism feedstuff, little growth speed of pigs can be improved.Test one group than matched group weightening finish raising 5.74%；Test two groups better, higher by 8.61% than matched group；
Containing abundant digestive enzyme in fermenting organism feedstuff, it is remarkably improved piglets to the digestibility of feedstuff, utilization rate, reduces feedstuff-meat ratio；Test one group and reduce by 4.33% than matched group, test two groups and reduce by 4.76% than matched group；
Big pig stage result of use in embodiment 3
By adding the fermenting organism feedstuff that a certain amount of embodiment 1 prepares in feedstuff, observe centering, big pig growth impact and production effect.
Middle pig is tested: selecting pig 80 in the DLY lean meat species health meat that parity, age in days, sex, body weight are close, be randomly divided into 4 hurdles, 20, every hurdle, two hurdles are one group, point matched group and test group, matched group: pig particulate material in purchasing with this；Test group: the particulate material that matched group formula+4% fermenting organism feedstuff is made..
Big pig is tested: test the big pig of quadrat method percent test and a packet with middle pig, matched group: purchase big pig particulate material with this；Test group: the particulate material that matched group formula+4% fermenting organism feedstuff is made.
Feed mode: free choice feeding, is advisable with slightly surplus material in morning next day, it is ensured that sufficient clean drinking-water, daily management, each hurdle of anti-epidemic measure are respectively organized all consistent.
Observed content: mental status；Fecaluria situation；Coat condition；
Data record: test head number, every daily material consumption, Average weight increasing a day, feedstuff-meat ratio etc..
By spirit, fecaluria, fur are contrasted, it can be seen that no matter be middle pig test group, or big pig test group, the feeding effect of test group is relatively better, the pig prothymia of test group；Not digesting feedstuff and niblet completely in feces less, stool odor is lighter；Fur is more suitable, more glow, searches for food, and weightening finish data are in Table 4.
Embodiment 4 nursing sow result of use
By adding the fermenting organism feedstuff that the embodiment 1 of 4% prepares in feedstuff, observe it to the growth of nursing sow and the impact producing performance.
Selection greatly enhances binary health weight tire sow 80, and parity, product age (antenatal 3 days) are close.
Matched group: this autogamy nursing sow powder
Test group: this autogamy nursing sow powder+4% fermenting organism feedstuff
Feed mode: according to this existing method, it is ensured that sufficient clean drinking-water.
Observed content: mental status, fecaluria state, coat condition, milk yield of sow, the piglets uniformity etc..
Data record: nursing sow feed intake, the ill head number of sow, sow postweaning estrus breeding rate and natural law, piglets Average weight increasing a day, piglets diarrhoea number of times, piglets survival rate.
Nursing sow spirit, fecaluria situation, coat condition, milk yield of sow, piglets uniformity contrast situation, the situation of test group is superior to matched group；Especially feces, fur improve comparatively obvious, and in test group feces, indigested niblet significantly reduces, and fur is also more suitable；The size of piglet and growth are more uniform, and nursing sow feed intake, piglets weightening finish data are in Table 5.
From the data of table 5 it can be seen that the fermenting organism feedstuff for preparing of embodiment 1 can the appetite of obvious stimulation animal, improve nursing sow feed intake, increase lactation amount, growth of suckling pigs is vigorous, and sow mastitis reduces；It is remarkably improved the nursing sow digestibility to feedstuff, utilization rate, improves sow milk water quality (especially in hot summer, when nursing sow feed intake is generally reduced, this effect is significant), be conducive to piglets healthy growth, reduce diarrhoea, improve survival rate.Test group piglets reduces by 52% than matched group diarrhoea number of times, and number of weaned improves 2.33%.
Meanwhile, containing a large amount of useful viable bacterias in fermenting organism feedstuff, swine alimentary canal microecological balance can effectively be adjusted；Organic acid in fermenting organism feedstuff can effectively suppress harmful microbe growth in sow body；In excrement, the content of harmful microorganism, ammonia, hydrogen sulfide and scatol substantially reduces；All these are all conducive to building the good the intestines and stomach microecological environment of sow and pig house environment, reduce the generation of the intestines and stomach, respiratory tract and hysteropathy, improve pig health level.
Claims (10)
1. the method that a multiple bacteria compound fermentation prepares fermenting organism feedstuff, it is characterised in that comprise the following steps:
S1. first time aerobic fermentation: obtain compound after manioc waste, wheat bran, bean cake, aspergillus niger and yeast being mixed, 28～30 DEG C of fermentation 4～5d；
S2. second time aerobic fermentation: add lactic acid bacteria and continue fermentation 1d；
S3. anaerobic fermentation: add wheat bran, TONGKANG, and add bacillus subtilis and lactic acid bacteria, be distributed in bag by the material unstrained spirits of mix homogeneously, after unlimited bag mouth places a period of time under room temperature, is evacuated and hermetically sealed bag mouth, continues heap fermentation 2～3 weeks under room temperature；
The source of described aspergillus niger is ACCC30162, and the source of yeast is BNCC336054, and the source of bacillus subtilis is ACCC10746, and the source of lactic acid bacteria is CICC20680.
2. preparation method according to claim 1, it is characterised in that in described compound, the mass fraction of manioc waste is 40～60%, and wheat bran is 0～2%, and bean cake is 0～4.5%, aspergillus niger 0.04～0.08%, and inoculum of dry yeast is 105～106Individual/g compound, surplus is water.
3. preparation method according to claim 1, it is characterised in that the inoculum concentration of lactic acid bacteria described in S2 is 106～107Individual/g compound.
4. preparation method according to claim 1, it is characterised in that the inoculum concentration of bacillus subtilis described in S3 and lactic acid bacteria is 10 respectively6～107Individual/g compound and 106Individual/g compound.
5. preparation method according to claim 1, it is characterised in that the moisture of the material unstrained spirits of mix homogeneously described in S3 is 45%.
6. preparation method according to claim 1, it is characterised in that S3 at room temperature opens wide bag mouth to place 7～8h, makes material unstrained spirits water content in bag be down to 25%.
7. the multiple bacteria compound fermentation biological feedstuff that method described in any one of claim 1 to 6 obtains.
8. the application in promoting pig growth and development of the multiple bacteria compound fermentation biological feedstuff described in claim 7.
9. multiple bacteria compound fermentation biological feedstuff application in promoting milking sow lactation amount, improving milk water quality according to claim 8.
10. application according to claim 8 or claim 9, it is characterised in that described multiple bacteria compound fermentation biological feedstuff and live pig solar eclipse feedstuff are mixed immediately feeding.
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| CN106819491A (en) * | 2017-04-07 | 2017-06-13 | 山东省农业科学院畜牧兽医研究所 | A kind of complete stool viridescent corn particle feed production method |
| CN106858038A (en) * | 2017-02-21 | 2017-06-20 | 内蒙古汉恩生物科技有限公司 | A kind of composite probiotic fermented feed and its manufacturing process |
| CN106912699A (en) * | 2017-01-21 | 2017-07-04 | 广西神龙王农牧食品集团有限公司 | A kind of pig feed for reducing pig manure stink and preparation method thereof |
| CN107897503A (en) * | 2017-11-24 | 2018-04-13 | 贵州大兴农业科技发展有限公司 | High-grade feed and preparation method thereof |
| CN107897529A (en) * | 2017-11-24 | 2018-04-13 | 贵州大兴农业科技发展有限公司 | Low anti-feed of Tiny ecosystem and preparation method thereof |
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| CN109452459A (en) * | 2018-11-07 | 2019-03-12 | 广西南农康顺生物开发有限公司 | A kind of potato fermenting organism mixed feed |
| CN112075530A (en) * | 2020-09-25 | 2020-12-15 | 广西科胜生物科技有限公司 | Preparation method and application of feed additive |
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| CN106561972A (en) * | 2016-10-09 | 2017-04-19 | 青岛琅琊台集团股份有限公司 | Method for producing biological feed rich in nucleic acid, DHA and proteins |
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| CN106819491A (en) * | 2017-04-07 | 2017-06-13 | 山东省农业科学院畜牧兽医研究所 | A kind of complete stool viridescent corn particle feed production method |
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| CN109452459A (en) * | 2018-11-07 | 2019-03-12 | 广西南农康顺生物开发有限公司 | A kind of potato fermenting organism mixed feed |
| CN112075530A (en) * | 2020-09-25 | 2020-12-15 | 广西科胜生物科技有限公司 | Preparation method and application of feed additive |
| CN112410201A (en) * | 2020-10-21 | 2021-02-26 | 青岛贝宝海洋科技有限公司 | Feed mixing equipment and control method thereof |
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Application publication date: 20160629 |