CN107897529A - Low anti-feed of Tiny ecosystem and preparation method thereof - Google Patents
Low anti-feed of Tiny ecosystem and preparation method thereof Download PDFInfo
- Publication number
- CN107897529A CN107897529A CN201711200693.9A CN201711200693A CN107897529A CN 107897529 A CN107897529 A CN 107897529A CN 201711200693 A CN201711200693 A CN 201711200693A CN 107897529 A CN107897529 A CN 107897529A
- Authority
- CN
- China
- Prior art keywords
- green
- feed
- time
- fermentate
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention relates to technique for producing feed field, and more particularly to low anti-feed of Tiny ecosystem and preparation method thereof；This method includes mixing feedstuff, replenishers, feed level microbe and green-tea extract, carries out first time fermentation, obtains first time fermentate；First time fermentate is deblocked, adds water and green-tea extract, second is carried out and ferments, obtain second of fermentate；Briquetting after second of fermentate is filtered, carries out third time fermentation, obtains third time fermentate；Third time fermentate is deblocked, adds green-tea extract, carries out the 4th fermentation；The preparation method can control the antibiotic in feed to maintain relatively low level, to improve the absorbability of livestock, shorten breeding cycle, promote livestock fast-growth, development etc..
Description
Technical field
The present invention relates to technique for producing feed field, and more particularly to low anti-feed of Tiny ecosystem and preparation method thereof.
Background technology
In the feeding process of livestock, situations such as growth to livestock of the use of feed, development, has large effect.But
Growth, development of the antibiotic contained in some existing feeds not only bad for livestock, so reduce the laying eggs of livestock,
Milk production, meat yield etc., antibiotic in feed it is also possible to the meat of livestock, milk, egg product in accumulate, and pass through food chain
Into in body so that human body Long Term Contact antibiotic, when resulting in the need for antibiotic and being treated, curative effect substantially reduces, very
The effect of extremely can not showing to have；Antibiotic in animal feeding-stuff, which is spread unchecked, can also cause environment no small influence, Ke Nengzeng
Add hydrogen sulfide in environment, the discharge capacity of ammonia, make the environmental degradation of farm, influence the health of poultry raiser.
The content of the invention
It is an object of the invention to provide a kind of preparation method of the low anti-feed of Tiny ecosystem, which can control feeding
Antibiotic content in material, makes to contain only a small amount of antibiotic in manufactured feed, and then manufactured feed is promoted domestic animal
The growth and development of poultry, can also avoid accumulation of the antibiotic in livestock body, avoid antibiotic from entering human body by food chain, with
Ensure human body when needing to be treated with antibiotic, antibiotic can give play to curative effect, additionally it is possible to reduce environment pollution and
Harm, reduces the discharge of antibiotic or bad air in environment.
Another object of the present invention is to provide a kind of Tiny ecosystem low anti-feed, which can improve the absorption energy of livestock
Power, shortens breeding cycle, promotes livestock fast-growth, reduces accumulation of the harmful substance in livestock body, improves livestock and lays eggs, produces
Breast amount etc..
The present invention is realized using following technical scheme.
The present invention proposes a kind of preparation method of the low anti-feed of Tiny ecosystem, it is included feedstuff, replenishers, feed grade
Microorganism and green-tea extract mixing, carry out first time fermentation, obtain first time fermentate.
First time fermentate is deblocked, adds water and green-tea extract, second is carried out and ferments, obtain second of fermentation
Thing.
Briquetting after second of fermentate is filtered, carries out third time fermentation, obtains third time fermentate.
Third time fermentate is deblocked, adds green-tea extract, the 4th fermentation is carried out, is added in third time fermentate
The weight ratio of green-tea extract and third time fermentate is 1:20-25, the temperature of the 4th fermentation is 20-25 DEG C.
Wherein, feedstuff is selected from least one of dregs of beans, rapeseed dregs, Cottonseed Meal and peanut meal, and replenishers are selected from micro-
At least one of secondary element, vitamin and amino acid；The weight ratio of feedstuff and replenishers is 50-60:1.
The present invention proposes a kind of low anti-feed of Tiny ecosystem, it is the preparation method preparation by the above-mentioned low anti-feed of Tiny ecosystem
's.
The beneficial effect of the low anti-feed of Tiny ecosystem of the embodiment of the present invention and preparation method thereof is：The preparation method can be controlled
Antibiotic content in feed processed, makes to contain only a small amount of antibiotic in manufactured feed, and then manufactured feed is promoted
Into the growth and development of livestock, accumulation of the antibiotic in livestock body can be also avoided, avoids antibiotic from entering people by food chain
Body, to ensure human body when needing to be treated with antibiotic, antibiotic can give play to curative effect, additionally it is possible to reduce the dirt of environment
Dye and harm, reduce the discharge of antibiotic or bad air in environment.The feed can improve the absorbability of livestock, shorten
Breeding cycle, promotes livestock fast-growth；Reduce accumulation of the harmful substance in livestock body, improve livestock lay eggs, milk production etc..
Embodiment
, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer, is the conventional production that can be obtained by commercially available purchase
Product.
Low anti-feed of Tiny ecosystem of the embodiment of the present invention and preparation method thereof is specifically described below.
The preparation method of the low anti-feed of Tiny ecosystem of the present invention includes：
Feedstuff, replenishers, feed level microbe and green-tea extract are mixed, carry out first time fermentation, obtains the
One time fermentation thing.
First time fermentate is deblocked, adds water and green-tea extract, second is carried out and ferments, obtain second of fermentation
Thing.
Briquetting after second of fermentate is filtered, carries out third time fermentation, obtains third time fermentate.
Third time fermentate is deblocked, adds green-tea extract, the 4th fermentation is carried out, is added in third time fermentate
The weight ratio of green-tea extract and third time fermentate is 1:20-25, the temperature of the 4th fermentation is 20-25 DEG C.
Feed is prepared according to the method described above, can be mould to avoid substantial amounts of penicillin, soil is produced during feed fermentation
The antibionts such as element, chloramphenicol, you can so as to contain only a small amount of antibiont by the feed that the preparation method is prepared.
Above-mentioned feedstuff is selected from least one of dregs of beans, rapeseed dregs, Cottonseed Meal and peanut meal, and replenishers are selected from micro
Element (such as：Iron, zinc etc.), vitamin (such as：Vitamin A, vitamin B and vitamin C etc.) and amino acid (such as：Rely ammonia
At least one of acid, arginine etc.)；The weight ratio of feedstuff and replenishers is 50-60:1.
Above-mentioned feed level microbe can be selected from least one of bacillus, lactic acid bacteria and saccharomycete.Need to illustrate
, the feed level microbe that uses can be commercially available microbe additive in the present invention, direct use can or
Homemade microbe additive.
The preparation method of foregoing green-tea extract includes：Green tea powder is added in the ethanol of 70-80vol%, be subsequently placed in
At a temperature of 50-60 DEG C, 30-60min is stirred, obtains the first mixture；First mixture is added water to cook, obtains the second mixing
Thing；Second mixture is filtered, obtains filtrate and filter residue, tea slurry is made in filter residue, tea slurry is made an addition in filtrate, then is dosed
20-30vol% ethanol decocts, and finally filters and concentrates in vacuo；Wherein, the weight ratio of the water of the first mixture addition and green tea powder
For 50-80:1, the weight ratio of green tea powder and 70-80vol% ethanol is 1:20-30.Green-tea extract is prepared according to the method described above,
On the one hand it can prevent the active principles such as the tea polyphenols in green tea powder, caffeine from being destroyed during extract is prepared, separately
On the one hand above-mentioned active principle can be extracted totally, improves the utilization rate of green tea materials.
Preferably, green tea powder and the weight ratio of 20-30vol% ethanol are 1:30-50；The green tea obtained after vacuum concentration
Extract environment temperature be 26-30 DEG C when, relative density 1.10.The temperature of above-mentioned vacuum concentration can be 45-55 DEG C,
Vacuum can be 0.01-0.02MPa.
The time that first mixture adds water to cook can be 2-3h；The time that addition 20-30vol% ethanol is decocted can
To be 1-2h.
The gross weight of above-mentioned feedstuff and replenishers and the green-tea extract added in the feedstuff and replenishers
Weight ratio be 15-25:1；Feed level microbe (commercially available microbe additive) and the weight ratio of green-tea extract can be 1:
1, and the microorganism concn contained in each parts by weight green-tea extract can be 1 × 105-1×107CFU/g；Ferment for the first time
Temperature can be 25-35 DEG C, the feed level microbe made an addition at this temperature in fermentation raw material can be activated, Jin Erke
To utilize the feed level microbe feed conversion raw material being activated, improve the nutrition-allocated proportion of feedstuff.Green-tea extract adds
Harmful microbe when fermenting for the first time can be reduced by, which adding, generates, and suppresses microorganism generation antibiotic.Fermentation for the first time
Time can be 12-24h.
The weight ratio of the green-tea extract added after the deblocking of first time fermentate and first time fermentate is 1:20-30；The
The temperature of secondary fermentation can be 20-25 DEG C, and fermentation time can be 10-12h.Relatively low after first time fermentate is deblocked
At a temperature of ferment, on the one hand the fermentation of fermentate can be made more sufficiently to carry out, on the other hand can be to avoid fermentation process
The middle substantial amounts of antibiotic of generation.The water added after first time fermentate is deblocked, can be the 1-2 of first time fermentate weight
Times.
The temperature of above-mentioned third time fermentation can be 25-30 DEG C, and the time of fermentation can be 8-10h.When third time is fermented,
Block fermentate is controlled at higher temperature and ferments, contribute to the conversion of fermentate in bulk by fermentate,
And contact of the fermentate with air can be reduced in block fermentate, and harmful microbe generation is on the one hand reduced, it is another
Aspect suppresses the generation of antibiotic.It should be noted that above-mentioned briquetting can be pressed with various existing press-forming machines
Block, such as：The briquetting machine of brick tea；It can also be that Manual pressurizing completes briquetting.
The time of foregoing 4th fermentation can be 10-12h, and the time of the 4th fermentation is unsuitable long, avoid when long
Between fermentation in generate substantial amounts of antibiotic.4th fermentate after fermentation can be used directly as feed, also may be used
By after the moisture evaporation in the 4th fermentate, feed powde or particle etc. is made.
Low anti-feed of Tiny ecosystem of the present invention and preparation method thereof is described in further detail with reference to embodiments.
Embodiment 1
Prepare green-tea extract：It is 1 by weight ratio:20 green tea powder and the ethanol mixing of 70vol%, are placed in 50 DEG C of temperature
Under degree, 60min is stirred, obtains the first mixture；The weight ratio that water and green tea powder are added into the first mixture is 50:1, decoct
2h (100 DEG C), obtains the second mixture；Second mixture is filtered, obtains filtrate and filter residue, tea slurry is made in filter residue, by tea
Slurry is made an addition in filtrate, is 1 according still further to green tea powder and the weight ratio of 20vol% ethanol:30 addition 20vol% ethanol decoct 2h
(100℃)；Finally filter, under the conditions of temperature is 45 DEG C, vacuum is 0.01MPa, be concentrated in vacuo, obtaining relative density is
1.10 (30 DEG C) green-tea extracts.
Ferment for the first time：It is 50 by weight ratio:1 feedstuff and replenishers mixing, feedstuff include dregs of beans, vegetable seed
The dregs of rice, Cottonseed Meal and peanut meal, replenishers include vitamin A, B, are extracted according to the gross weight of feedstuff and replenishers and green tea
The weight ratio of thing is 15:1, green-tea extract is added, and be 1 according to the weight ratio of green-tea extract and lactic acid bacteria:1 makes an addition to feeding
In the mixture for expecting raw material, replenishers and green-tea extract, and the microorganism concn contained in each parts by weight green-tea extract
Can be 1 × 105CFU/g, ferment 12h at 35 DEG C.
Second of fermentation：After first time fermentate is deblocked, 1 times of water of first time fermentate weight is added in for the first time
In fermentate, then 1/20 green-tea extract of first time fermentate weight is added, ferment 12h under the conditions of 25 DEG C.
Third time is fermented：Briquetting after second of fermentate is filtered, ferment 10h under the conditions of 30 DEG C.
4th fermentation：Third time fermentate is deblocked, adds 1/20 green-tea extract of third time fermentate weight,
Ferment 12h under the conditions of 25 DEG C.
Embodiment 2
Prepare green-tea extract：It is 1 by weight ratio:30 green tea powder and the ethanol mixing of 80vol%, are placed in 60 DEG C of temperature
Under degree, 30min is stirred, obtains the first mixture；The weight ratio that water and green tea powder are added into the first mixture is 80:1, decoct
3h (98 DEG C), obtains the second mixture；Second mixture is filtered, obtains filtrate and filter residue, tea slurry is made in filter residue, tea is starched
Make an addition in filtrate, be 1 according still further to green tea powder and the weight ratio of 30vol% ethanol:50 addition 30vol% ethanol decoct 1h (98
℃)；Finally filter, under the conditions of temperature is 55 DEG C, vacuum is 0.02MPa, be concentrated in vacuo, obtain relative density as 1.10
(26 DEG C) green-tea extract.
Ferment for the first time：It is 60 by weight ratio:1 feedstuff and replenishers mixing, feedstuff include Cottonseed Meal and
Peanut meal, replenishers include vitamin A and lysine, according to the weight of the gross weight and green-tea extract of feedstuff and replenishers
Amount is than being 25:1, green-tea extract is added, and be 1 according to the weight ratio of green-tea extract and saccharomycete:1 makes an addition to feed original
Material, replenishers and green-tea extract mixture in, and the microorganism concn contained in each parts by weight green-tea extract can be with
It is 1 × 107CFU/g, ferment 24h at 25 DEG C.
Second of fermentation：After first time fermentate is deblocked, 2 times of water of first time fermentate weight is added in for the first time
In fermentate, then 1/30 green-tea extract of first time fermentate weight is added, ferment 10h under the conditions of 20 DEG C.
Third time is fermented：Briquetting after second of fermentate is filtered, ferment 8h under the conditions of 25 DEG C.
4th fermentation：Third time fermentate is deblocked, adds 1/25 green-tea extract of third time fermentate weight,
Ferment 10h under the conditions of 20 DEG C.
Embodiment 3
Prepare green-tea extract：It is 1 by weight ratio:25 green tea powder and the ethanol mixing of 75vol%, are placed in 55 DEG C of temperature
Under degree, 40min is stirred, obtains the first mixture；The weight ratio that water and green tea powder are added into the first mixture is 60:1, decoct
2.5h (99 DEG C), obtains the second mixture；Second mixture is filtered, obtains filtrate and filter residue, tea slurry is made in filter residue, by tea
Slurry is made an addition in filtrate, is 1 according still further to green tea powder and the weight ratio of 25vol% ethanol:40 addition 25vol% ethanol decoct 1.5h
(99℃)；Finally filter, under the conditions of temperature is 50 DEG C, vacuum is 0.01MPa, be concentrated in vacuo, obtaining relative density is
1.10 (27 DEG C) green-tea extracts.
Ferment for the first time：It is 55 by weight ratio:1 feedstuff and replenishers mixing, feedstuff is dregs of beans, replenishers
For trace element, the weight ratio according to the gross weight and green-tea extract of feedstuff and replenishers is 20:1, addition green tea carries
Thing is taken, and is 1 according to the weight ratio of green-tea extract and lactic acid bacteria and the mixture of saccharomycete:1 makes an addition to feedstuff, supplement
In the mixture of agent and green-tea extract, and the microorganism concn contained in each parts by weight green-tea extract can be 1 ×
106CFU/g, ferment 18h at 30 DEG C.
Second of fermentation：After first time fermentate is deblocked, 1.5 times of water of first time fermentate weight is added in first
In secondary fermentate, then 1/25 green-tea extract of first time fermentate weight is added, ferment 11h under the conditions of 22 DEG C.
Third time is fermented：Briquetting after second of fermentate is filtered, ferment 9h under the conditions of 27 DEG C.
4th fermentation：Third time fermentate is deblocked, adds 1/22 green-tea extract of third time fermentate weight,
Ferment 11h under the conditions of 22 DEG C.
Embodiment 4
Prepare green-tea extract：It is 1 by weight ratio:22 green tea powder and the ethanol mixing of 72vol%, are placed in 56 DEG C of temperature
Under degree, 50min is stirred, obtains the first mixture；The weight ratio that water and green tea powder are added into the first mixture is 70:1, decoct
2.2h (99 DEG C), obtains the second mixture；Second mixture is filtered, obtains filtrate and filter residue, tea slurry is made in filter residue, by tea
Slurry is made an addition in filtrate, is 1 according still further to green tea powder and the weight ratio of 22vol% ethanol:40 addition 22vol% ethanol decoct
1.2h；Finally filter, under the conditions of temperature is 52 DEG C, vacuum is 0.02MPa, be concentrated in vacuo, obtain relative density as 1.10
(28 DEG C) green-tea extract.
Ferment for the first time：It is 53 by weight ratio:1 feedstuff and replenishers mixing, feedstuff is peanut meal, supplement
Agent is iron, zinc and arginine, and the weight ratio according to the gross weight and green-tea extract of feedstuff and replenishers is 22:1, addition
Green-tea extract, and be 1 according to the weight ratio of green-tea extract and lactic acid bacteria:1, which makes an addition to feedstuff, replenishers and green tea, carries
Take in the mixture of thing, and the microorganism concn contained in each parts by weight green-tea extract can be 6 × 105CFU/g, 32
Ferment 16h at DEG C.
Second of fermentation：After first time fermentate is deblocked, 1.8 times of water of first time fermentate weight is added in first
In secondary fermentate, then 1/27 green-tea extract of first time fermentate weight is added, ferment 10.5h under the conditions of 23 DEG C.
Third time is fermented：Briquetting after second of fermentate is filtered, ferment 10h under the conditions of 28 DEG C.
4th fermentation：Third time fermentate is deblocked, adds 1/24 green-tea extract of third time fermentate weight,
Ferment 11.5h under the conditions of 24 DEG C.
Embodiment 5
Prepare green-tea extract：It is 1 by weight ratio:30 green tea powder and the mixing of 100 DEG C of hot water, in 100 DEG C of condition
Lower decoction 1h, filtering.
Ferment for the first time：It is 58 by weight ratio:1 feedstuff and replenishers mixing, feedstuff is peanut meal, supplement
Agent is iron, zinc and arginine, and the weight ratio according to the gross weight and green-tea extract of feedstuff and replenishers is 24:1, addition
Green-tea extract, and be 1 according to the weight ratio of green-tea extract and bacillus:1 makes an addition to feedstuff, replenishers and green tea
In the mixture of extract, and the microorganism concn contained in each parts by weight green-tea extract can be 5 × 106CFU/g,
Ferment 20h at 27 DEG C.
Second of fermentation：After first time fermentate is deblocked, 1.6 times of water of first time fermentate weight is added in first
In secondary fermentate, then 1/24 green-tea extract of first time fermentate weight is added, ferment 12h under the conditions of 21 DEG C.
Third time is fermented：Briquetting after second of fermentate is filtered, ferment 10h under the conditions of 26 DEG C.
4th fermentation：Third time fermentate is deblocked, adds 1/21 green-tea extract of third time fermentate weight,
Ferment 10h under the conditions of 25 DEG C.
Comparative example 1
Comparative example 1 prepare feed method and embodiment 1 it is similar, difference is, when comparative example 1 is fermented,
In feedstuff, replenishers and feed level microbe add green-tea extract after, direct fermentation 46h, do not carry out multiple deblocking,
Briquetting fermentation procedure, other parameters, step can be directly with reference to embodiments 1.
Comparative example 2
Comparative example 2 prepare feed method and embodiment 1 it is similar, difference is, first has only been carried out in comparative example 2
Secondary fermentation and second of fermentation, the time of second of fermentation is 34h, does not carry out follow-up briquetting, deblocking fermentation, other processes
Carried out with parameter with reference to embodiment 1.
Comparative example 3
The method that comparative example 3 prepares feed is similar to Example 1, and difference is, being not added with green tea in comparative example 3 carries
Thing is taken, remaining process and parameter etc. are carried out with reference to embodiment 1.
Test example 1
Detect the content of antibiotic in the low anti-feed of Tiny ecosystem that embodiment 1-5 and comparative example 1-3 is provided.Testing index
For chloramphenicol, the method for detection is with reference to GB/T 8381.9-2005《The measure of chloramphenicol in feed》Carry out.Testing result is shown in Table
1。
The content (‰) of chloramphenicol in 1 each group feed of table
From the results shown in Table 1, the content of the chloramphenicol in the feed that prepared by preparation method of the invention is substantially low
In comparative example 1-3, low anti-feed can be prepared by illustrating the method for the present invention.
From the comparison of embodiment 1-5 and comparative example 1,2 can be seen that the present invention carry out repeatedly deblocking, briquetting fermentation can be with
Reduce the generation of antibiotic in feed.From embodiment 1-5 and comparative example 3 as can be seen that the present invention is when carrying out the fermentation of feed
Add green-tea extract, it is possibility to have beneficial to the generation for reducing antibiotic in feed.
Test example 2
The feed prepared with embodiment 1-5 and comparative example 1-3, carries out the feeding experiment of livestock.It is similar to choose growth conditions
Milk cow 80, be randomly divided into 8 groups, every group 10, feed feed prepared by embodiment 1-5 and comparative example 1-3 respectively, raise daily
Feed 2 times, each every above-mentioned all feeds of Cow-feeding 3kg, after feeding 20d, observation below 5d (while the statistics output of milk after
It is continuous to feed each milk cow according to the method described above), the average daily average milk production of every milk cow.It the results are shown in Table 2.
The average daily output of milk (kg) of the every milk cow of table 2
As shown in Table 2, feed of the invention can significantly improve the milk producing ability of milk cow, illustrate the preparation method of the present invention
The feed of preparation can promote growth and development and galactopoiesis ability of livestock etc..From embodiment 1-5 and the comparing result of comparative example 1-2
Understand, the present invention can improve absorbability of the livestock to feed using the method for gradation, deblocking, briquetting fermentation, promote livestock
Growth and development etc.；Comparing embodiment 1-5 and comparative example 3 are understood, are added green-tea extract when preparing feed, can be improved domestic animal
The absorption to nutriment is raiseeed, promotes growth, the development, yield etc. of livestock.
In conclusion the beneficial effect of low anti-feed of the Tiny ecosystem of the embodiment of the present invention and preparation method thereof is：The preparation
Method can control the antibiotic content in feed, make to contain only a small amount of antibiotic in manufactured feed, and then make manufactured feed
It can not only promote the growth and development of livestock, can also avoid accumulation of the antibiotic in livestock body, avoid antibiotic from passing through food
Thing chain enters human body, and to ensure human body when needing to be treated with antibiotic, antibiotic can give play to curative effect, additionally it is possible to subtract
The pollution and harm of few environment, reduce the discharge of antibiotic or bad air in environment.The feed can improve the suction of livestock
Receipts ability, shortens breeding cycle, promotes livestock fast-growth；Accumulation of the harmful substance in livestock body is reduced, improves livestock production
Egg, milk production.
Embodiments described above is part of the embodiment of the present invention, instead of all the embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
1. the preparation method of the low anti-feed of a kind of Tiny ecosystem, it is characterised in that including feedstuff, replenishers, feed grade is micro-
Biology and green-tea extract mixing, carry out first time fermentation, obtain first time fermentate；
The first time fermentate is deblocked, adds water and the green-tea extract, second is carried out and ferments, obtain second of hair
Ferment thing；
Briquetting after second of fermentate is filtered, carries out third time fermentation, obtains third time fermentate；
The third time fermentate is deblocked, adds the green-tea extract, the 4th fermentation is carried out, adds in third time fermentate
The weight ratio of the green-tea extract added and the third time fermentate is 1:20-25, the temperature of the 4th fermentation are
20-25℃；
Wherein, the feedstuff is selected from least one of dregs of beans, rapeseed dregs, Cottonseed Meal and peanut meal, the replenishers choosing
From at least one of trace element, vitamin and amino acid；The weight ratio of the feedstuff and the replenishers is 50-
60:1。
2. the preparation method of the low anti-feed of Tiny ecosystem according to claim 1, it is characterised in that the green-tea extract
Preparation method includes：Green tea powder is added in the ethanol of 70-80vol%, at a temperature of being subsequently placed in 50-60 DEG C, stir 30-
60min, obtains the first mixture；First mixture is added water to cook, obtains the second mixture；By second mixture
Filtering, obtains filtrate and filter residue, and the filter residue is made tea slurry, tea slurry is made an addition in the filtrate, then dose 20-
30vol% ethanol decocts, and finally filters and concentrates in vacuo；Wherein,
The water and the weight ratio of the green tea powder of the first mixture addition are 50-80:1, the green tea powder and the 70-
The weight ratio of 80vol% ethanol is 1:20-30.
3. the preparation method of the low anti-feed of Tiny ecosystem according to claim 2, it is characterised in that the green tea powder with it is described
The weight ratio of 20-30vol% ethanol is 1:30-50.
4. the preparation method of the low anti-feed of Tiny ecosystem according to claim 2, it is characterised in that after the vacuum concentration
The green-tea extract arrived environment temperature be 26-30 DEG C when, relative density 1.10.
5. the preparation method of the low anti-feed of Tiny ecosystem according to claim 1, it is characterised in that the feedstuff and institute
The gross weight for stating replenishers is with the weight ratio for making an addition to the green-tea extract in the feedstuff and the replenishers
15-25:1。
6. the preparation method of the low anti-feed of Tiny ecosystem according to claim 1, it is characterised in that the first time fermentation
Temperature is 25-35 DEG C.
7. the preparation method of the low anti-feed of Tiny ecosystem according to claim 1, it is characterised in that the first time fermentate
The weight ratio of the green-tea extract added after deblocking and the first time fermentate is 1:20-30.
8. the preparation method of the low anti-feed of Tiny ecosystem according to claim 1, it is characterised in that second of fermentation
Temperature is 20-25 DEG C, fermentation time 10-12h.
9. the preparation method of the low anti-feed of Tiny ecosystem according to claim 1, it is characterised in that the third time fermentation
Temperature is 25-30 DEG C, fermentation time 8-10h.
10. the low anti-feed of a kind of Tiny ecosystem, it is characterised in that it is by the low anti-feeding of any one of the claim 1-9 Tiny ecosystems
Prepared by the preparation method of material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711200693.9A CN107897529A (en) | 2017-11-24 | 2017-11-24 | Low anti-feed of Tiny ecosystem and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711200693.9A CN107897529A (en) | 2017-11-24 | 2017-11-24 | Low anti-feed of Tiny ecosystem and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107897529A true CN107897529A (en) | 2018-04-13 |
Family
ID=61848742
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711200693.9A Pending CN107897529A (en) | 2017-11-24 | 2017-11-24 | Low anti-feed of Tiny ecosystem and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107897529A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1161785A (en) * | 1996-04-10 | 1997-10-15 | 四川农业大学饮料新技术研究开发中心 | Technology for processing tea drink without muddy liquid |
| CN105707434A (en) * | 2016-02-01 | 2016-06-29 | 广州市科胜生物科技有限公司 | Method and application of fermented biological feed prepared by multi-strain mixed fermentation |
| CN105994946A (en) * | 2016-06-06 | 2016-10-12 | 广东大广生物科技有限公司 | Preparation method of high calcium lactate fermented feed |
| CN106666112A (en) * | 2016-12-27 | 2017-05-17 | 薄文海 | Fattening feed for donkeys |
| CN107183366A (en) * | 2017-04-25 | 2017-09-22 | 蚌埠市和平乳业有限责任公司 | A kind of Dairy Cows in Late Lactation special feed |
-
2017
- 2017-11-24 CN CN201711200693.9A patent/CN107897529A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1161785A (en) * | 1996-04-10 | 1997-10-15 | 四川农业大学饮料新技术研究开发中心 | Technology for processing tea drink without muddy liquid |
| CN105707434A (en) * | 2016-02-01 | 2016-06-29 | 广州市科胜生物科技有限公司 | Method and application of fermented biological feed prepared by multi-strain mixed fermentation |
| CN105994946A (en) * | 2016-06-06 | 2016-10-12 | 广东大广生物科技有限公司 | Preparation method of high calcium lactate fermented feed |
| CN106666112A (en) * | 2016-12-27 | 2017-05-17 | 薄文海 | Fattening feed for donkeys |
| CN107183366A (en) * | 2017-04-25 | 2017-09-22 | 蚌埠市和平乳业有限责任公司 | A kind of Dairy Cows in Late Lactation special feed |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103250874B (en) | Preparation method of apple pomace fermented feed | |
| CN104892086B (en) | A kind of compound seaweed fertilizer and preparation method thereof | |
| CN107319148A (en) | A kind of growth promotion ecological immunity type sika deer special feed and preparation method thereof | |
| CN109076882A (en) | A kind of mycelial cultural method of selenium-enriched edible mushroom and its application | |
| CN109287865A (en) | A kind of composite fermentation protein feed and preparation method thereof | |
| CN103564199A (en) | Method for producing cow feed by multi-strain mixing solid-state fermentation | |
| CN109105678A (en) | A kind of feeding additive aquatic animal and preparation method thereof | |
| CN106387336A (en) | Corn slurry fermented feed and production method thereof | |
| CN104232728B (en) | Rapeseed peptide (rsp) albumen and preparation method thereof | |
| CN100417335C (en) | Composite life beneficial bacteria culture for improving milk cow production performance and its making method | |
| CN108522790A (en) | A kind of method of solid state fermentation tealeaf residue production fiber feedstuff | |
| CN110959748A (en) | Liquid fermented feed prepared from waste vegetables and preparation method thereof | |
| CN102499331B (en) | Dairy cow rumen regulating agent and preparation method thereof | |
| CN109007304A (en) | Promote prawn shelling mixed feed | |
| CN109497262A (en) | Utilize the method for wet basis fermentation raw material preparation sheep feed and the sheep feed of preparation | |
| CN105124285A (en) | Ecological duck feed | |
| CN104855678A (en) | Method for producing tricin by utilizing viable bacteria glycolysis | |
| CN103483058A (en) | Formula and manufacturing method of coprinus comatus culture material | |
| CN107897529A (en) | Low anti-feed of Tiny ecosystem and preparation method thereof | |
| CN108719030A (en) | A kind of preparation method of ferro-rich bean sprouts, ferro-rich bean sprouts feed and its preparation method and application | |
| CN108094708A (en) | A kind of feed addictive of the slag containing Cortex Eucommiae and preparation method thereof | |
| CN107897503A (en) | High-grade feed and preparation method thereof | |
| CN106721091A (en) | A kind of soft-shelled turtle feed of utilization cassava grain stillage production novel microbial fermentation and preparation method thereof | |
| CN106615965A (en) | Myriophyllum elatinoides feed for ducks and preparation method thereof | |
| CN104855689A (en) | Method for preparing fatty acid-rich protein feed raw material from amino acid fermentation residual liquor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180413 |
|
| RJ01 | Rejection of invention patent application after publication |