CN104855678A - Method for producing tricin by utilizing viable bacteria glycolysis - Google Patents
Method for producing tricin by utilizing viable bacteria glycolysis Download PDFInfo
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- CN104855678A CN104855678A CN201510301781.2A CN201510301781A CN104855678A CN 104855678 A CN104855678 A CN 104855678A CN 201510301781 A CN201510301781 A CN 201510301781A CN 104855678 A CN104855678 A CN 104855678A
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- tricin
- powder
- alfalfa hay
- alfalfa
- hay powder
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Abstract
The invention belongs to the technical field of separation of natural plant extracts and relates to a method for producing tricin by utilizing viable bacteria glycolysis. The method comprises the following specific steps: 1, performing impurity-removing and drying on alfalfa and then crushing to obtain alfalfa hay powder; 2, adding 4-5 percent of corn flour and 4-5 percent of protein powder of the total weight of the alfalfa hay powder as auxiliary materials; 3, sugaring compound probiotics which take brevibacterium as a main component to prepare special fermented liquid; 4, uniformly mixing and stirring the alfalfa hay powder, the auxiliary materials and the fermented liquid according to the proportion of 50-55 percent of moisture content of a final mixture, then putting into a mobile fermentation bag and fermenting for 15-20 days to obtain wet powder containing the tricin or drying to obtain the alfalfa hay powder. About 30 percent of tricin is contained in the fermented alfalfa hay powder, and through a lot of feeding trying tests, the effect of direct wet powder feeding is better than that of dry powder feeding. The method is simple and quick in tricin production, less in link, low in cost, full in raw material utilization, free from energy consumption, three wastes and residues, environment-friendly and green and free from pollution and public nuisance.
Description
Technical field
The invention belongs to natural plant extracts separation technology field.Relate to a kind of extracting method of Tricin, particularly relate to the method taking viable bacteria glycolysis biotechnology to produce Tricin.
Background technology
Alfalfa is perennial leguminous herbaceous plant, extensively plants all over the world, and also there is planting base all parts of the country of China, mainly uses as albumen herbage.In recent years, along with the application of yellow celery polysaccharide in livestock breeding industry, its result of use is good, remains, have no side effect, be day by day subject to the welcome of aquaculture without antibody.But its use cost is high, makes again it be difficult to large-scale popularization in the middle of cultivation and use.And the appearance of Tricin just in time addresses this problem, in alfalfa, Tricin content is more, the clover of extracting from alfalfa
elementmain component is Alfalfa Polysaccharide, and Alfalfa Polysaccharide is through the feeding experiment of national correlation department, and effect is very good, and its price is also lower than astragalus polyose many.Along with people's improving constantly meat, eggs and milk quality requirements, widely using of Tricin is arrived increasingly.
The method of traditional extraction Tricin is poach leaching method in the market, and its method is: rear pulverizing is dried in Alfalfa impurity elimination, adds clean clear water, boil 2 hours by 12 times of alfalfa hay powder.Carry out Separation of Solid and Liquid after being placed into room temperature, parting liquid is inserted in container and is clarified, get supernatant through multistage be concentrated into the liquid of about 20% after to carry out whitewashing net belt type dry, dry thing is pulverized and obtains Tricin powder.This method is widely used traditional Chinese medicine extraction method in the market, though belong to comparatively advanced extractive technique, but the investment of this method is large, water consumption is large, big energy-consuming, length consuming time, extract link too complicated, cost is high, and this method governs Tricin low price widely using in aquaculture at present.Therefore, finding economical and practical Tricin extracting method is the task of top priority.
Summary of the invention
For current Tricin extracting method present situation, the object of the invention is to provide a kind of fast easy, the Tricin new process for producing that cost is low.
For realizing the object of the invention, the present invention takes viable bacteria glycolysis biotechnology to produce Tricin.Realize especially by following method.
1 Alfalfa impurity elimination is dried after, pulverize, obtain alfalfa hay powder.
2 add the corn flour of each 4-5% of alfalfa hay powder gross weight and large protein powder as auxiliary material.
3, by based on the compound probiotic of withered grass gemma, add molasses and are mixed with fermenting liquid; Compound probiotic and molasses weight ratio are 1:20.
4, by alfalfa hay powder, auxiliary material and the zymotic fluid ratio mixing and stirring in final mixture water content 50-55%, then load in mobile fermentation bag, fermentation 15-20 days, and wet-milling or the drying that must contain Tricin obtain alfalfa hay powder.
Described compound probiotic is formed so that percentage by weight is composite by following component:
Candida utili 30%
Bacillus subtilis 30%
Lactic acid bacteria 30%
Photosynthetic bacteria 5%
Bifidobacterium Bifidum 5%.
The principle of the invention is: during the fermentation, and the cell membrane that compound probiotic constantly destroys alfalfa hay powder makes it discharge a large amount of Alfalfa Polysaccharides and organic substance, and its method is far longer than Alfalfa Polysaccharide and the organic substance of the release of poach leaching method.
The invention has the advantages that: containing the Tricin (calculating with dry) of about 30% in the alfalfa meal fermented, the direct wet-milling of Tricin that can be used as low concentration uses or makes dry powder after low temperature drying and uses.Feed test through a large amount of examination, the effect that direct wet-milling is fed is better than dry powder effect, the feeding effect after the Tricin being more better than high concentration dilutes again.This method produces that Tricin is simple, fast, link is few, cost is low and raw material utilizes fully, without power consumption, without the three wastes, nothing remnants, environment-friendly and green, pollution-free, nuisanceless.
Detailed description of the invention
Embodiment 1
1, after Alfalfa impurity elimination being dried, pulverize, obtain alfalfa hay powder; Take 100kg alfalfa hay powder and add the corn flour of 5kg and the large protein powder of 5kg.
2, by based on the compound probiotic of withered grass gemma, add molasses and be mixed with fermenting liquid; Compound probiotic and molasses weight ratio are 1:20.
Described compound probiotic is formed so that percentage by weight is composite by following component
Candida utili 30%
Bacillus subtilis 30%
Lactic acid bacteria 30%
Photosynthetic bacteria 5%
Bifidobacterium Bifidum 5%.
3, add the above-mentioned zymotic fluid of 100kg, in the ratio mixing and stirring of final mixture water content 50-55%, load mobile fermentation bag, ferment 15 days.The fermentation of producing 210kg is wet alfalfa hay powder, or low temperature drying obtains the alfalfa hay powder of 110kg, includes the Tricin of about 30 kilograms after testing.
Through adjusting: extract expense only 30 yuan, and existing extracting method: by the extraction expense of 2000 yuan/raw material per ton, extract take=200 yuan.
This method economic benefit is very remarkable.Fast easy, low cost, is convenient to promote, Tricin can be made to be used widely in livestock breeding industry.
Application examples
The wet-milling of the Tricin prepared by above-described embodiment is fed pig, tests as follows.
The pig of 100 about 25 kilograms is divided into two groups at random, control group adds the obtained dry Tricin of percentage by weight 2% traditional Chinese medicine extraction method at conventional feed, another group is added percentage by weight 2%(dry and is calculated in conventional feed) the wet Tricin that obtains of the present invention, all feed by method at regular time and quantity for two groups, feed every day twice.Feeding time 100 days, daily feeding observes the state of mind of pig, adopts the situation of raising and premunition, weighs after 100 days, adjusts feeding effect.
Feeding effect contrasts: result experimental group has following advantage than control group:
1. adopt at every turn raise time shorten 10-15 minutes;
2. pig is full of vitality, and fur is shinny, and gloss is ruddy;
3. the injection of 3 monthly ages is once after blue ear vaccine, and antibody titer is high by 53%;
4. the omnidistance incidence of disease is lower than 71.5%;
5. last many 513 kilograms of gross weight.
Claims (1)
1. utilize viable bacteria glycolysis to produce the method for Tricin, it is characterized in that, realize by the following method:
(1), after Alfalfa impurity elimination being dried, pulverize, obtain alfalfa hay powder;
(2) corn flour of each 4-5% of alfalfa hay powder gross weight and large protein powder is added as auxiliary material;
(3) by based on the compound probiotic of withered grass gemma, add molasses and be mixed with fermenting liquid; Compound probiotic and molasses weight ratio are 1:20;
(4) by alfalfa hay powder, auxiliary material and the zymotic fluid ratio mixing and stirring in final mixture water content 50-55%, then load in mobile fermentation bag, fermentation 15-20 days, wet-milling or the drying that must contain Tricin obtain alfalfa hay powder; Described compound probiotic is formed so that percentage by weight is composite by following component:
Candida utili 30%
Bacillus subtilis 30%
Lactic acid bacteria 30%
Photosynthetic bacteria 5%
Bifidobacterium Bifidum 5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510301781.2A CN104855678A (en) | 2015-06-05 | 2015-06-05 | Method for producing tricin by utilizing viable bacteria glycolysis |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510301781.2A CN104855678A (en) | 2015-06-05 | 2015-06-05 | Method for producing tricin by utilizing viable bacteria glycolysis |
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| CN104855678A true CN104855678A (en) | 2015-08-26 |
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| CN201510301781.2A Pending CN104855678A (en) | 2015-06-05 | 2015-06-05 | Method for producing tricin by utilizing viable bacteria glycolysis |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106551168A (en) * | 2016-11-30 | 2017-04-05 | 辽宁禾丰牧业股份有限公司 | A kind of mixed fermentation fiber feedstuff for laying hen feeding and preparation method thereof |
| CN106721261A (en) * | 2016-11-30 | 2017-05-31 | 辽宁禾丰牧业股份有限公司 | One kind is used for swine rearing mixed fermentation fiber feedstuff and preparation method thereof |
| CN107551167A (en) * | 2017-10-12 | 2018-01-09 | 南京壹唯壹生物科技有限公司 | Purposes of Spartina alterniflora's extract in blood uric acid functional product is prepared |
| CN110651893A (en) * | 2019-11-22 | 2020-01-07 | 山西省农业科学院高寒区作物研究所 | Novel alfalfa pig feed |
Citations (2)
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| CN1139520A (en) * | 1996-03-05 | 1997-01-08 | 高银相 | Production method for biological multi-bacterial fermented fodder made from stalks |
| CN1530437A (en) * | 2003-03-14 | 2004-09-22 | 新疆威仕达生物工程股份有限公司 | Microbial ensiling strain and composite fungus, method for producing silage |
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2015
- 2015-06-05 CN CN201510301781.2A patent/CN104855678A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1139520A (en) * | 1996-03-05 | 1997-01-08 | 高银相 | Production method for biological multi-bacterial fermented fodder made from stalks |
| CN1530437A (en) * | 2003-03-14 | 2004-09-22 | 新疆威仕达生物工程股份有限公司 | Microbial ensiling strain and composite fungus, method for producing silage |
Non-Patent Citations (7)
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| B.A.JONES ETAL: "Influence of bacterial inoculant and substrate addition", 《GRASS AND FORAGE》 * |
| 杨俊峰: "乳酸菌在青贮中的应用及研究进展", 《营养与日粮》 * |
| 梁忠纪: "《百日出栏养猪法》", 31 May 2015, 科学技术文献出版社 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106551168A (en) * | 2016-11-30 | 2017-04-05 | 辽宁禾丰牧业股份有限公司 | A kind of mixed fermentation fiber feedstuff for laying hen feeding and preparation method thereof |
| CN106721261A (en) * | 2016-11-30 | 2017-05-31 | 辽宁禾丰牧业股份有限公司 | One kind is used for swine rearing mixed fermentation fiber feedstuff and preparation method thereof |
| CN107551167A (en) * | 2017-10-12 | 2018-01-09 | 南京壹唯壹生物科技有限公司 | Purposes of Spartina alterniflora's extract in blood uric acid functional product is prepared |
| CN110651893A (en) * | 2019-11-22 | 2020-01-07 | 山西省农业科学院高寒区作物研究所 | Novel alfalfa pig feed |
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Application publication date: 20150826 |