CN103211084A - Method for preparing biological enzyme-enriched camellia seed meal fermented feed - Google Patents
Method for preparing biological enzyme-enriched camellia seed meal fermented feed Download PDFInfo
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Abstract
The invention discloses a method for preparing biological enzyme-enriched camellia seed meal fermented feed. The method comprises the following steps: detoxifying camellia seed meal, mixing the camellia seed meal with bean pulp and bran, inoculating trichoderma viride CGMCC NO.33711 and bacillus subtilis CGMCC NO.11630, and performing solid state fermentation; and finally, performing turbine stirring cyclone drying and crushing the obtained product, and obtaining the camellia seed meal fermented feed. Most of antinutritional factors in the camellia seed meal are reduced, the contents of crude protein and soluble protein are improved, lots of biological enzymes for promoting digestive absorption of animals are produced, the digestive absorption capacity of the animals is improved, the feed utilization efficiency is improved, and the production performance of the animals is improved further; and meanwhile, the microecological balance in livestock and poultry bodies is regulated, the growth of pathogenic bacteria is inhibited, the disease resistance of the animals is enhanced, and the animals can be helped to reduce the application of antibiotics.
Description
Technical field
The present invention relates to microorganism and animal nutrition and feed technical field, be specifically related to a kind of preparation method of camellia seed meal fermented feed.
Utilizing the production technology of Trichoderma viride and bacillus subtilis hybrid solid ferment oil leached tea oil slag, is a kind of by adjusting fermentation basigamy ratio and regulating and control the production method that biology enzyme feeding oil leached tea oil slag is rich in the fermentation condition preparation.
Background technology
Oil tea is grown in the high mountain and the hilly country of subtropical zone, China south, is the distinctive a kind of pure natural special oil tannin plant of China, accounts for more than 80% of national woody edible oil materials.China's oil tea area has fifty-five million mu approximately, and about 1,000,000 tons of tea seed annual productions are produced about 200,000 tons of tea oil per year, about 11,000,000,000 yuan of the output value.By oil tea tea seed oil content is 26%～39%, and it is 70% calculating that machine presses oil yield, about 730,000 tons of camellia seed meal annual production, and, greatly develop camellia oleiferaindustry because the production structure adjustment in tea district is produced in the whole nation, the camellia seed meal annual production increases year by year.
ANFs too high levels such as Tea Saponin, polyphenols in the camellia seed meal that tradition milling process system oil is produced, be not suitable for being directly used in animal and produce, major part is used as pool-cleaning agent, fertilizer, fuel, even discarded, be used for the extraction of Tea Saponin on a small quantity, few part is as feed.If remove the ANFs in the camellia seed meal, just can make it become the higher potential feedstuff of a kind of nutritive value.Owing to be subjected to the influence of tea seed kind, producing region distribution and collecting season, oil tea extraction process etc., the nutrient composition content of camellia seed meal changes greatly.The camellia seed meal of carrying behind the oil generally contains 0.5%～7% crude fat, 10%～20% protein, 15%～25% crude fibre, 30%～60% glucide, 20%～50% NFE, the digestible energy of 8～10 MJ/kg.Contain 18 seed amino acids in the camellia seed meal protein, comprise 10 kinds of essential amino acids that growth of animals or poultry is required, wherein threonine, glutamic acid, histidine and arginine equal size are than horn of plenty.Simultaneously, mineral matter and trace elements of Ca, K, Mg, Fe, Mn equal size are also than horn of plenty.
Want camellia seed meal is dropped into feeding, just must carry out detoxification treatment, traditional physics and chemical lixiviate detoxification, ubiquity shortcomings such as production cost height, detoxification are not thorough, nutrient loss, in the very difficult practice that puts into production on a large scale, so must have one effectively at the detoxification process and the biological modification of camellia seed meal composition with the milling process change.
About the technology of domestic biofermentation camellia seed meal, report to some extent: horsepower adopts bacillus licheniformis, aspergillus niger, saccharomyces uvarum and aspergillus oryzae mixed fungus fermentation in " the ferment oil CHAKUBING is produced the screening of the mixed bacteria of protein feed " and " protein feed oleifera dry cake detoxification and fermentation medium primary dcreening operation "; The Zhou Yue adopts the fermentation of mould list bacterium in " screening of the bacterial classification of degraded oil tea saponin and the optimization of condition of culture thereof "; Zhou Haoyu adopts the chemical method detoxification in " comparison of fermentation method and chemical method improvement camellia seed meal quality effect "; With bacillus subtilis, aspergillus niger and candida utili Mixed Microbes solid state fermentation; Ren Xiane selects for use candida tropicalis, bacillus subtilis and aspergillus niger to mix the bacterium solid state fermentation in " the camellia seed meal solid state fermentation is produced the research of animal feeding-stuff containing somatic protein "; Deng Zeyuan adopts sturdy crassa list bacterium ferment oil leached tea oil slag; Hu Long adopts mixed fungus fermentation method ferment oil leached tea oil slag to produce single cell protein, and fermentation back crude protein content is 18.33%; , fermentation back crude protein content has improved 15% ~ 40%.The mixed bacteria that Deng Guilan " the mixed fermentation oil tea dregs of rice are produced the research of animal feeding-stuff containing somatic protein " forms with mould and saccharomycete is a fermented bacterium, with different culture medium solid state fermentations.
Above-mentioned research still has the following disadvantages in mixed bacterium solid state fermentation camellia seed meal process as prior art:
At first be that microbial metabolic products can cause antagonism each other, influences ferment effect owing to the bacterial classification difference, our screening test in early stage has also proved this point;
Secondly because simple camellia seed meal is not the good culture medium of microorganism, have the carbon-nitrogen ratio of a rational microbial reproduction simultaneously reducing production costs, make and select base-material that big difficulty is arranged with the camellia seed meal mix and match;
Secondly in order to obtain being rich in the camellia seed meal fermented feed of biology enzyme, can not use the camellia seed meal of Tea Saponin or secondary oil expression of having purified, can only use once and not extracting the camellia seed meal of Tea Saponin as fermentation raw material after the oil expression again;
Owing to still there is a spot of Tea Saponin to exist, make that the later camellia seed meal viscosity of fermentation is bigger in addition, when drying, lump easily that effect is relatively poor.Therefore the later drying process of detoxification is selected also very important.
Summary of the invention
Technical problem to be solved by this invention provides a kind of preparation method who is rich in the camellia seed meal fermented feed of biology enzyme, make the ANFs in its camellia seed meal of can effectively degrading, improve protein quality, improve the animal digestion absorbability, effectively improve breeding performonce fo animals.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is: be rich in the preparation method of the camellia seed meal fermented feed of biology enzyme, may further comprise the steps:
(1) will mix the back lixiviate through the camellia seed meal of once oil expression with distilled water, and discard supernatant liquid, drying obtains the detoxification camellia seed meal;
(2) bacillus subtilis that will derive from the Trichoderma viride that is numbered CGMCCCNO.33711 at China Committee for Culture Collection of Microorganisms common micro-organisms center and be numbered CGMCCCNO.11630 activates on the inclined-plane solid medium respectively, insert again and shake bottle expansion cultivation, make seed liquor; Described Trichoderma viride adopts the PDA culture medium, and described bacillus subtilis is adopted beef-protein medium;
(3) detoxification camellia seed meal described in (1) and dregs of beans, wheat bran are mixed, Trichoderma viride in the inoculation (2) and bacillus subtilis seed liquor are carried out solid state fermentation and are obtained tunning, with described tunning drying and crushing, obtain fermented feed then.
As preferably, mix the back lixiviates in 1:3～6 by volume through camellia seed meal and the distilled water that once extracts oil in the step (1), water temperature is 50～80 ℃, extraction time 4～7h.
As preferably, Trichoderma viride seed liquor concentration is 2.00 * 10 in the step (2)
8～3.00 * 10
8Cfu/ml, bacillus subtilis seed liquor concentration is 3.00 * 10
8～4.00 * 10
8Cfu/ml.
As preferably, detoxification camellia seed meal and dregs of beans, wheat bran press mass ratio 4～6:3～4:0.5～1 mixing in the step (3), and fermentation temperature is 26～33 ℃, and pH is 5～8, and fermentation time is 48～96h.
As preferably, in the step (3) seed liquor and mixed detoxification camellia seed meal, dregs of beans and the wheat bran part by weight by 1～5:100 is mixed, wherein Trichoderma viride and bacillus subtilis mix by the volume ratio of 3～7:5 in the seed liquor.
As preferably, detoxification camellia seed meal, dregs of beans and wheat bran all pass through high-temperature sterilization, and sterilising temp is 121 ℃, and the time is 25～35 min.
As preferably, the drying of tunning described in step (1) detoxification camellia seed meal and the step (3) adopts turbine stirring cyclone drying process, and baking temperature is not more than 60 ℃, and dry back moisture is 10～15%.Why adopt turbine stirring cyclone drying, purpose is to solve fermentation later on owing to still having a small amount of Tea Saponin to have the problem that caking takes place, because this technology rate of drying is fast, can reduce the material loss of ferment oil leached tea oil slag simultaneously.
As preferably, the constituent of PDA culture medium is potato 200g in the step (2), glucose 20g, distilled water 1000ml.
As preferably, the constituent of beef-protein medium is beef extract 3g in the step (2), peptone 10g, sodium chloride 5g, distilled water 1000ml.
Compared with prior art, beneficial effect of the present invention is:
1. the present invention is by the detoxification of first physics hot water, turbine stirring cyclone drying process, use the method for Trichoderma viride and bacillus subtilis mixed solid fermentation camellia seed meal then, reduced most of ANFs in the camellia seed meal, crude protein and soluble protein content have been improved, and produced and helped the biological enzyme that animal digestion absorbs in a large number, help to improve the ability of digesting and assimilating of animal, improve the utilization ratio of feed, and then improve breeding performonce fo animals.Regulate microecological balance in the animal body simultaneously, suppressed the pathogen growth, strengthened the animal disease resistant ability, can appropriateness help animal to reduce antibiotic use.
2. the present invention selects Trichoderma viride and bacillus subtilis on the single bacterium fermentation of multiple microorganism, mixed fungus fermentation experimental study basis; Adopt the combined ferment in the mixed fungus fermentation, Trichoderma viride and bacillus subtilis are inoculated simultaneously and cultivate, make Trichoderma viride and the bacillus subtilis can cooperative fermentation, bring into play positive combined effect during the fermentation, having remedied single bacterium fermentation and some mixed fungus fermentation has mutual inhibiting deficiency, helps the accumulation of the solid state fermentation product of camellia seed meal.
3. the present invention is simple to operate, and nontoxic no animal metabolism thing pollutes, and can reduce aquaculture cost significantly, turns waste into wealth.
The specific embodiment
Specify the present invention below in conjunction with 4 embodiment.
Embodiment 1:
1) the 5kg camellia seed meal is added the distilled water of 4 times of volumes, be placed in the water-bath of 60 ℃ of water temperatures lixiviate 6 hours, per half stirs once when slight, and it is dry under 50 ℃ of temperature to discard supernatant liquid then.
2) Trichoderma viride seed liquor concentration is 2.00 * 10
8Cfu/ml, bacillus subtilis seed liquor concentration is 3.00 * 10
8Cfu/ml.
3) the detoxification camellia seed meal that obtains mixes by the mass ratio of 5:4:1 with dregs of beans, wheat bran, and the mixed bacteria liquid that inoculates 1 % is put into fermenter and fermented, and the volume ratio of Trichoderma viride and bacillus subtilis is 5:5 in the mixed bacteria liquid.The fermentation bung is opened wide and is covered sterile gauze, and fermentation temperature is 30 ℃, and pH is 7, fermentation time be the every 24h stirring of 72h(once).After the fermentation ends, tunning is put into drying under 50 ℃ of temperature, pulverize and obtain ferment oil leached tea oil slag feed.
After measured, the ferment oil leached tea oil slag feed that said method produces, after the fermentation ends, crude protein content has improved 34%, and water-soluble protein content has improved 28%, little peptide content has improved 235%, Tea Saponin content has reduced by 82%, and tannin content has reduced by 75%, simultaneously, cellulase activity is 1482U/g in the fermented feed, and neutral proteinase is lived and is 4128U/g.
Embodiment 2:
1) the 5kg camellia seed meal is added the distilled water of 3 times of volumes, be placed in the water-bath of 50 ℃ of water temperatures lixiviate 6 hours, per half stirs once when slight, and it is dry under 50 ℃ of conditions to discard supernatant liquid then.
2) Trichoderma viride seed liquor concentration is 3.00 * 10
8Cfu/ml, bacillus subtilis seed liquor concentration is 3.00 * 10
8Cfu/ml.
3) the detoxification camellia seed meal that obtains mixes by the mass ratio of 5.5:4:0.5 with dregs of beans, wheat bran, and the mixed bacteria liquid that inoculates 3 % is put into fermenter and fermented, and the volume ratio of Trichoderma viride and bacillus subtilis is 4:5 in the mixed bacteria liquid.The fermentation bung is opened wide and is covered sterile gauze, and fermentation temperature is 26 ℃, and pH is 6, fermentation time be the every 24h stirring of 72h(once).After the fermentation ends, tunning is put into drying under 50 ℃ of temperature, pulverize and obtain ferment oil leached tea oil slag feed.
After measured, the ferment oil leached tea oil slag feed that said method produces, after the fermentation ends, crude protein content has improved 35%, and water-soluble protein content has improved 24%, little peptide content has improved 265%, Tea Saponin content has reduced by 88%, and tannin content has reduced by 77%, simultaneously, cellulase activity is 1566U/g in the fermented feed, and neutral proteinase is lived and is 4355U/g.
Embodiment 3:
1) the 5kg camellia seed meal is added the distilled water of 5 times of volumes, be placed in the water-bath of 70 ℃ of water temperatures lixiviate 4 hours, per half stirs once when slight, and it is dry under 50 ℃ of conditions to discard supernatant liquid then.
2) Trichoderma viride seed liquor concentration is 2.00 * 10
8Cfu/ml, bacillus subtilis seed liquor concentration is 4.00 * 10
8Cfu/ml.
3) the detoxification camellia seed meal that obtains mixes by the mass ratio of 4:3:1 with dregs of beans, wheat bran, and the mixed bacteria liquid that inoculates 4 % is put into fermenter and fermented, and the volume ratio of Trichoderma viride and bacillus subtilis is 3:5 in the mixed bacteria liquid.The fermentation bung is opened wide and is covered sterile gauze, and fermentation temperature is 33 ℃, and pH is 8, fermentation time be the every 24h stirring of 72h(once).After the fermentation ends, tunning is put into drying under 50 ℃ of conditions, pulverize and obtain ferment oil leached tea oil slag feed.
After measured, the ferment oil leached tea oil slag feed that said method produces, after the fermentation ends, crude protein content has improved 29%, and water-soluble protein content has improved 30%, little peptide content has improved 223%, Tea Saponin content has reduced by 90%, and tannin content has reduced by 71%, simultaneously, cellulase activity is 1316U/g in the fermented feed, and neutral proteinase is lived and is 4412U/g.
Embodiment 4:
1) the 5kg camellia seed meal is added the distilled water of 6 times of volumes, be placed in the water-bath of 80 ℃ of water temperatures lixiviate 4 hours, per half stirs once when slight, and it is dry under 50 ℃ of temperature to discard supernatant liquid then.
2) Trichoderma viride seed liquor concentration is 3.00 * 10
8Cfu/ml, bacillus subtilis seed liquor concentration is 4.00 * 10
8Cfu/ml.
3) the detoxification camellia seed meal that obtains mixes by the mass ratio of 6:4:0.5 with dregs of beans, wheat bran, inoculates 5% mixed bacteria liquid and puts into fermenter and ferment, and the volume ratio of Trichoderma viride and bacillus subtilis is 7:5 in the mixed bacteria liquid.The fermentation bung is opened wide and is covered sterile gauze, and fermentation temperature is 28 ℃, and pH is 6, fermentation time be the every 24h stirring of 72h(once).After the fermentation ends, tunning is put into drying under 50 ℃ of temperature, pulverize and obtain ferment oil leached tea oil slag feed.
After measured, the ferment oil leached tea oil slag feed that said method produces, after the fermentation ends, crude protein content has improved 34%, and water-soluble protein content has improved 27%, little peptide content has improved 247%, Tea Saponin content has reduced by 94%, and tannin content has reduced by 82%, simultaneously, cellulase activity is 1421U/g in the fermented feed, and neutral proteinase is lived and is 4653U/g.
In the above-described embodiments, relevant data detects according to following country or industry standard:
Crude protein content is carried out according to standard GB/T6432-94 " crude protein assay method in the feed ";
Water-soluble protein content is carried out according to agricultural industry criteria NY/T1205-2006 " mensuration of soybean water-soluble protein content ";
Little peptide content is carried out according to light industry standard QB/T2653-2004 " soy peptide powder ";
Tea Saponin content is carried out according to high performance liquid chromatography (Tan Bo etc., 2009);
Tannin content is carried out according to feedstuff industry standard SN/T0800.9-1999 " importing and exporting grain, the feed tannin content method of inspection ";
Cellulase activity content is carried out according to agricultural industry criteria NY/T912-2004 " the mensuration AAS of feed addictive cellulase activity ";
Neutral proteinase content alive is carried out according to commodity inspection industry standard SB/T10317-1999 " protease activity amylograph ".
The above; only be the preferable specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to replacement or change according to technical scheme of the present invention and inventive concept thereof, all should be encompassed within protection scope of the present invention.
Claims (9)
1. be rich in the preparation method of the camellia seed meal fermented feed of biology enzyme, it is characterized in that, may further comprise the steps:
(1) will mix the back lixiviate through the camellia seed meal of once oil expression with distilled water, and discard supernatant liquid, drying obtains the detoxification camellia seed meal;
(2) bacillus subtilis that will derive from the Trichoderma viride that is numbered CGMCCCNO.33711 at China Committee for Culture Collection of Microorganisms common micro-organisms center and be numbered CGMCCCNO.11630 activates on the inclined-plane solid medium respectively, insert again and shake bottle expansion cultivation, make seed liquor; Described Trichoderma viride adopts the PDA culture medium, and described bacillus subtilis is adopted beef-protein medium;
(3) detoxification camellia seed meal described in (1) and dregs of beans, wheat bran are mixed, Trichoderma viride in the inoculation (2) and bacillus subtilis seed liquor are carried out solid state fermentation and are obtained tunning, with described tunning drying and crushing, obtain fermented feed then.
2. camellia seed meal fermented feed preparation method as claimed in claim 1 is characterized in that: camellia seed meal and distilled water through once oil expression described in the step (1) mix the back lixiviate in 1:3～6 by volume, and water temperature is 50～80 ℃, extraction time 4～7h.
3. camellia seed meal fermented feed preparation method as claimed in claim 1 is characterized in that: Trichoderma viride seed liquor concentration is 2.00 * 10 in the step (2)
8～3.00 * 10
8Cfu/ml, bacillus subtilis seed liquor concentration is 3.00 * 10
8～4.00 * 10
8Cfu/ml.
4. camellia seed meal fermented feed preparation method as claimed in claim 1, it is characterized in that detoxification camellia seed meal and dregs of beans, wheat bran described in the step (3) are pressed mass ratio 4～6:3～4:0.5～1 and mixed, fermentation temperature is 26～33 ℃, pH is 5～8, and fermentation time is 48～96h.
5. camellia seed meal fermented feed preparation method as claimed in claim 1, it is characterized in that, in the step (3) seed liquor and mixed detoxification camellia seed meal, dregs of beans and the wheat bran part by weight by 1～5:100 is mixed, wherein Trichoderma viride and bacillus subtilis mix by the volume ratio of 3～7:5 in the seed liquor.
6. camellia seed meal fermented feed preparation method as claimed in claim 1 is characterized in that described detoxification camellia seed meal, dregs of beans and wheat bran all pass through high-temperature sterilization, and sterilising temp is 121 ℃, and the time is 25～35 min.
7. camellia seed meal fermented feed preparation method as claimed in claim 1, it is characterized in that, the drying of tunning described in step (1) detoxification camellia seed meal and the step (3) adopts turbine stirring cyclone drying process, and baking temperature is not more than 60 ℃, and dry back moisture is 10～15%.
8. camellia seed meal fermented feed preparation method as claimed in claim 1 is characterized in that the constituent of PDA culture medium is potato 200g described in the step (2), glucose 20g, distilled water 1000ml.
9. camellia seed meal fermented feed preparation method as claimed in claim 1 is characterized in that the constituent of beef-protein medium is beef extract 3g described in the step (2), peptone 10g, sodium chloride 5g, distilled water 1000ml.
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Cited By (12)
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| CN103598416A (en) * | 2013-11-26 | 2014-02-26 | 广东轻工职业技术学院 | Fermented soybean meal taking crusted tea as raw material as well as preparation method and application thereof |
| CN104365996A (en) * | 2014-12-12 | 2015-02-25 | 遵义市金鼎农业科技有限公司 | Method for preparing feedstuff by removing toxin of tea seed cakes or tea seed dregs in probiotics fermentation manner |
| CN105028920A (en) * | 2015-08-05 | 2015-11-11 | 安徽大别山科技开发有限公司 | High-quality tea dreg used for feed adding and preparing method thereof |
| CN106360689A (en) * | 2016-08-30 | 2017-02-01 | 柳州职业技术学院 | Tea bran ferment using brown sugar and preparation method of tea bran ferment |
| CN107259101A (en) * | 2017-02-13 | 2017-10-20 | 中南林业科技大学 | A kind of method of camellia seed meal ferment making feed |
| CN109709334A (en) * | 2018-11-26 | 2019-05-03 | 中国农业科学院油料作物研究所 | A method of analysis oil plant dregs of rice mineral acts in fermentation of bacillus production iraq subtilis actinomycin A |
| CN110384183A (en) * | 2019-09-02 | 2019-10-29 | 佛山播恩生物科技有限公司 | A kind of fermentation leached tea oil slag biological feedstuff and preparation method thereof |
| CN111019996A (en) * | 2020-01-18 | 2020-04-17 | 林丽芳 | Method for preparing active polypeptide by liquid fermentation of camellia seed meal |
| CN112806478A (en) * | 2021-01-18 | 2021-05-18 | 衡阳师范学院 | Production and application effect evaluation of microbial protein feed mainly containing tea seed meal |
| CN113729120A (en) * | 2021-08-26 | 2021-12-03 | 湖南金浩粮油工业有限公司 | Preparation method of tea oil cake meal probiotic pig feed |
| CN117448212A (en) * | 2023-10-20 | 2024-01-26 | 青蛙王子（福建）婴童护理用品有限公司 | Camellia seed fermented oil with moisturizing effect and preparation method and application thereof |
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| CN103598416A (en) * | 2013-11-26 | 2014-02-26 | 广东轻工职业技术学院 | Fermented soybean meal taking crusted tea as raw material as well as preparation method and application thereof |
| CN103598416B (en) * | 2013-11-26 | 2015-09-02 | 广东轻工职业技术学院 | A kind of take Chen Xiangcha as fermented bean dregs of raw material and preparation method thereof and application |
| CN104365996A (en) * | 2014-12-12 | 2015-02-25 | 遵义市金鼎农业科技有限公司 | Method for preparing feedstuff by removing toxin of tea seed cakes or tea seed dregs in probiotics fermentation manner |
| CN105028920A (en) * | 2015-08-05 | 2015-11-11 | 安徽大别山科技开发有限公司 | High-quality tea dreg used for feed adding and preparing method thereof |
| CN106360689A (en) * | 2016-08-30 | 2017-02-01 | 柳州职业技术学院 | Tea bran ferment using brown sugar and preparation method of tea bran ferment |
| CN107259101A (en) * | 2017-02-13 | 2017-10-20 | 中南林业科技大学 | A kind of method of camellia seed meal ferment making feed |
| CN109709334A (en) * | 2018-11-26 | 2019-05-03 | 中国农业科学院油料作物研究所 | A method of analysis oil plant dregs of rice mineral acts in fermentation of bacillus production iraq subtilis actinomycin A |
| CN109709334B (en) * | 2018-11-26 | 2022-04-01 | 中国农业科学院油料作物研究所 | Method for analyzing effect of mineral substances in oil meal in fermentation production of iturin A by bacillus |
| CN110384183A (en) * | 2019-09-02 | 2019-10-29 | 佛山播恩生物科技有限公司 | A kind of fermentation leached tea oil slag biological feedstuff and preparation method thereof |
| CN111019996A (en) * | 2020-01-18 | 2020-04-17 | 林丽芳 | Method for preparing active polypeptide by liquid fermentation of camellia seed meal |
| CN112806478A (en) * | 2021-01-18 | 2021-05-18 | 衡阳师范学院 | Production and application effect evaluation of microbial protein feed mainly containing tea seed meal |
| CN112806478B (en) * | 2021-01-18 | 2022-08-02 | 衡阳师范学院 | Production and application effect evaluation of microbial protein feed mainly containing tea seed meal |
| CN113729120A (en) * | 2021-08-26 | 2021-12-03 | 湖南金浩粮油工业有限公司 | Preparation method of tea oil cake meal probiotic pig feed |
| CN117448212A (en) * | 2023-10-20 | 2024-01-26 | 青蛙王子（福建）婴童护理用品有限公司 | Camellia seed fermented oil with moisturizing effect and preparation method and application thereof |
| CN118020867A (en) * | 2024-03-28 | 2024-05-14 | 广东省农业科学院动物科学研究所 | Solid state fermentation method of oil tea meal, feed additive and application |
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