CN1332173A - Prepn of theasaponine - Google Patents
Prepn of theasaponine Download PDFInfo
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- CN1332173A CN1332173A CN 01113794 CN01113794A CN1332173A CN 1332173 A CN1332173 A CN 1332173A CN 01113794 CN01113794 CN 01113794 CN 01113794 A CN01113794 A CN 01113794A CN 1332173 A CN1332173 A CN 1332173A
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- water
- tea saponin
- preparation
- impurity
- tea
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Abstract
The chemical process of extracting theasaponine from camellia seed oil cake includes the steps of: soaking camellia seed oil cake in 10-20 weight times water for 24-48 hr; heating to boil while stirring; filtering insoluble impurity and washing the impurity with boiled water for 1-5 times; vibrating yeast fermentation at 25-32 deg.c for 24-72 hr; centrifugal separation to eliminate impurity to obtain clear liquid; adding 3 times of ethanol to the clear liquid and stirring for 15 min; centrifugal separation after cooling; and concentration, decoloring and drying to obtain product.
Description
One, technical field
The present invention a kind ofly extracts the method for tea saponin with chemical process from the tea-seed dregs of rice, belong to the chemical purification technical field of tea saponin.
Two, background technology
Contain a certain amount of tea saponin in the later grouts of tea-seed oil expression, though because of the tea tree breed difference, higher as Qimen paper mulberry leaf kind, Lingui large leaf and southern river large leaf content, the content of other tea tree is all more than 8%.Tea saponin has the anti-inflammatory analgesic of eliminating the phlegm cough-relieving and many-sided curative effect such as antibiotic aspect pharmacology.Tea saponin belongs to the tensio-active agent of non-ionic type, and it is subjected to the influence of water hardness hardly, has very strong foaming power.It also has wettability tea saponin.Also have decontamination, emulsification and disperse many-sided characteristic.Therefore tea saponin is widely used in and makes shampoo, paraffin emulsifier, concrete suds-stabilizing agent and agricultural chemicals wetting agent.The extraction of tea saponin at present has water seaoning, its shortcoming usually: in leaching process, some water miscible sugar and protein also can be extracted out, thereby have influenced the quality of tea saponin.Another kind is to adopt the organic solvent lixiviation process, and its shortcoming is the cost height, loses more than gain.
Three, summary of the invention
The object of the present invention is to provide the preparation method of a kind of low cost, purity height, the simple tea saponin of extraction process.
The objective of the invention is to realize: utilize tea saponin can be dissolved in the characteristic of hot water with following method, adopt the low water of cost to come lixiviate, adopt yeast to ferment to remove sugar and protein in the tea saponin, ethanol with easy recovery is made with extra care tea saponin, carries out then conventional concentrating, decolouring, drying process obtain product.
Concrete method is:
(1) soaks leached tea oil slag in water bubble 24～48 hours;
(2) heated and boiled stirs simultaneously;
(3) filter undissolved impurity, and wash this undissolved impurity 1-5 time with boiling water;
(4) add yeast in solution, the vibration fermentation is 24～72 hours under 25-32 ℃ of temperature;
(5) remove impurity with the method for centrifugation, obtain clear liquid;
(6) in above-mentioned clear liquid, add 3 times ethanol, stirred 15 minutes;
(7) cooling back centrifugation;
(8) after concentrated, decolouring, drying, obtain product.
When being soaked in water leached tea oil slag, the per kilogram leached tea oil slag adds 10～20 liters in water.Concentrate and adopt the rotatory evaporator precipitation to concentrate.Adopt H
2O
2The processing of decolouring.
The cost that the invention has the advantages that extraction is low, purity is high, and extraction process is simple, does not pollute, and can be adapted to large-scale and small-sized industrial scale.
Four, the specific embodiment
Leached tea oil slag (the 1Kg leached tea oil slag adds 10～20 liters in water) under water was soaked 1～2 day, heat then and boil, and constantly stirred Tea Saponin fully is dissolved in the water, filter while hot elimination end dissolved impurity, wash undissolved impurity 1～5 time with boiling water, merge solution, join by ratio yeast 50 grams, in 25～32 ℃ of constant temperature vibration fermentations 1～3 day, centrifugation, get supernatant liquor and add 3 times of ethanol, carry out stirring in 5～10 minutes, sub-cooled is carried out centrifugation again, liquid is concentrated with the rotary evaporator precipitation, use H2O
2The processing of decolouring, use again vacuum drying product.
Claims (4)
1. the preparation method of a tea saponin is characterized in that the method for extracting is:
(1) soak leached tea oil slag in water bubble 24～48 hours:
(2) heated and boiled stirs simultaneously;
(3) filter undissolved impurity, and wash this undissolved impurity 1-5 time with boiling water;
(4) add yeast in solution, the vibration fermentation is 24～72 hours under 25-32 ℃ of temperature;
(5) remove impurity with the method for centrifugation, obtain clear liquid;
(6) in above-mentioned clear liquid, add 3 times ethanol, stirred 15 minutes;
(7) cooling back centrifugation;
(8) after concentrated, decolouring, drying, obtain product.
2. the preparation method of tea saponin according to claim 1, when it is characterized in that being soaked in water leached tea oil slag, the per kilogram leached tea oil slag adds 10～20 liters in water.
3. the preparation method of tea saponin according to claim 1 and 2 is characterized in that concentrating and adopts the rotatory evaporator precipitation to concentrate.
4. the preparation method of tea saponin according to claim 1 and 2 is characterized in that adopting H
2O
2The processing of decolouring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01113794 CN1332173A (en) | 2001-07-18 | 2001-07-18 | Prepn of theasaponine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01113794 CN1332173A (en) | 2001-07-18 | 2001-07-18 | Prepn of theasaponine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1332173A true CN1332173A (en) | 2002-01-23 |
Family
ID=4660499
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01113794 Pending CN1332173A (en) | 2001-07-18 | 2001-07-18 | Prepn of theasaponine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1332173A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101817865A (en) * | 2010-04-20 | 2010-09-01 | 浙江大学 | Method for extracting and separating tea saponin and feeding proteoglycan by using residue-removed oil-tea camellia cake |
| CN101880307A (en) * | 2010-06-17 | 2010-11-10 | 安徽省农业科学院茶叶研究所 | Method for extracting tea saponin by utilizing microbes |
| CN103211084A (en) * | 2013-04-17 | 2013-07-24 | 安徽农业大学 | Method for preparing biological enzyme-enriched camellia seed meal fermented feed |
| CN103276040A (en) * | 2013-05-23 | 2013-09-04 | 安徽省华银茶油有限公司 | Method for extracting tea saponin through fermentation |
| CN103739638A (en) * | 2013-12-25 | 2014-04-23 | 中华全国供销合作总社杭州茶叶研究所 | Method for purifying tea saponin through microbial fermentation |
| CN104031109A (en) * | 2014-05-29 | 2014-09-10 | 中华全国供销合作总社杭州茶叶研究所 | Method for purifying tea saponin by microbial fermentation |
| CN112194700A (en) * | 2020-10-09 | 2021-01-08 | 张利民 | Preparation method of natural high-purity tea saponin with high decontamination efficiency |
| CN112205424A (en) * | 2020-10-10 | 2021-01-12 | 江西应用技术职业学院 | Camellia oleifera cake cypress microbial metabolite and preparation method thereof |
-
2001
- 2001-07-18 CN CN 01113794 patent/CN1332173A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101817865A (en) * | 2010-04-20 | 2010-09-01 | 浙江大学 | Method for extracting and separating tea saponin and feeding proteoglycan by using residue-removed oil-tea camellia cake |
| CN101817865B (en) * | 2010-04-20 | 2013-08-14 | 浙江大学 | Method for extracting and separating tea saponin and feeding proteoglycan by using residue-removed oil-tea camellia cake |
| CN101880307A (en) * | 2010-06-17 | 2010-11-10 | 安徽省农业科学院茶叶研究所 | Method for extracting tea saponin by utilizing microbes |
| CN101880307B (en) * | 2010-06-17 | 2013-04-03 | 安徽省农业科学院茶叶研究所 | Method for extracting tea saponin by utilizing microbes |
| CN103211084A (en) * | 2013-04-17 | 2013-07-24 | 安徽农业大学 | Method for preparing biological enzyme-enriched camellia seed meal fermented feed |
| CN103276040A (en) * | 2013-05-23 | 2013-09-04 | 安徽省华银茶油有限公司 | Method for extracting tea saponin through fermentation |
| CN103739638A (en) * | 2013-12-25 | 2014-04-23 | 中华全国供销合作总社杭州茶叶研究所 | Method for purifying tea saponin through microbial fermentation |
| CN103739638B (en) * | 2013-12-25 | 2015-08-12 | 中华全国供销合作总社杭州茶叶研究所 | A kind of method of fermentable purifying tea saponin |
| CN104031109A (en) * | 2014-05-29 | 2014-09-10 | 中华全国供销合作总社杭州茶叶研究所 | Method for purifying tea saponin by microbial fermentation |
| CN112194700A (en) * | 2020-10-09 | 2021-01-08 | 张利民 | Preparation method of natural high-purity tea saponin with high decontamination efficiency |
| CN112205424A (en) * | 2020-10-10 | 2021-01-12 | 江西应用技术职业学院 | Camellia oleifera cake cypress microbial metabolite and preparation method thereof |
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