CN106551168A - A kind of mixed fermentation fiber feedstuff for laying hen feeding and preparation method thereof - Google Patents
A kind of mixed fermentation fiber feedstuff for laying hen feeding and preparation method thereof Download PDFInfo
- Publication number
- CN106551168A CN106551168A CN201611078965.8A CN201611078965A CN106551168A CN 106551168 A CN106551168 A CN 106551168A CN 201611078965 A CN201611078965 A CN 201611078965A CN 106551168 A CN106551168 A CN 106551168A
- Authority
- CN
- China
- Prior art keywords
- alfalfa
- seed liquor
- mixed
- raw mixture
- citrus pulp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of mixed fermentation fiber feedstuff for laying hen feeding and preparation method thereof, the feedstuff is prepared by the raw material of following parts by weight：Citrus pulp, oat bran, 1 part of the yeast mixed fermentation product A of Alfalfa, citrus pulp, oat bran, 13 parts of the lactic acid bacteria mixed fermentation product B of Alfalfa, citrus pulp, oat bran, 23 parts of the bacillus cereuss mixed fermentation product C of Alfalfa.Fermented by the mixing of citrus pulp, oat bran, Alfalfa, the intestinal villus development of chicken and Crypt depth can be significantly improved in chicken feed as a kind of fermentable fiber feed applications, improve digestive function and intestinal health state.
Description
Technical field
The invention belongs to fodder compound technical field, and in particular to a kind of oat bran and citrus pulp fiber fermentation feedstuff and
Preparation method.
Background technology
Feed fibre is primarily referred to as typically being difficult digested dietary nutrient, mostlys come from the cell wall of plant, bag
Cellulose, cellulose, natural gum, pectin and lignin etc..Fiber is a kind of complicated mixture, can according to water miscible difference
To be divided into water-soluble fibre (SDF) and water-insoluble fiber (IDF)；In recent years, dietary fiber be further divided into can fermented type it is fine
Peacekeeping can not be fermented fiber type.Water insoluble dietary fiber is mainly made up of plant cell wall, and such as cellulose, lignin and half are fine
Dimension element, sesame seed meal, Testa Tritici, rice bran meal etc., are present in the feed in a large number.Water soluble dietary fiber is by Hemicellulose Polysaccharide group
Into the pectin being such as present in Herba bromi japonici, Fructus Hordei Vulgaris, beans and gel etc..Which is generally hydrophilic, non-crystalline type fiber, with carbon aquation
Compound is relevant with the metabolism of lipid, easily by intestinal liquid moistening, therefore is easy to be utilized by the Institute of Micro-biology in intestinal.Can fermented type
Fiber refers to the part fiber that varying level degree can be resolved into by the microorganism in animal and bird intestines, mainly including levan,
Pectin, polydextrose and resistant starch etc..Can fermented type dietary fiber can promote poultry metabolism, such as its can as prebioticses,
Promote the growth of beneficial bacteria of intestinal tract；Short-chain fatty acid (SCFA) is produced, promotes the absorption of water and sodium, promote mucosal cell proliferation；
Energy is provided；Reduce the pH of cell intracavity.Can not fermented type dietary fiber mainly include cellulose, lignin and some fermentation
Slow hemicellulose.Can not fermented type dietary fiber be propagation which can promote microorganism to the Main Function of poultry,
So that microbial cleaning, degrade noxious substance and reach the purpose of removing toxic substances, and some microorganisms can also suppress or slow down
The propagation of cancerous cell；In addition, can not fermented type dietary fiber can also loosening bowel to relieve constipation, promote intestinal peristalsis promoting, reduce food in gastrointestinal
The dead time in road, reduce enteral pressure.The function of fiber：Used as a kind of special nutrient, it contains the biological activity of uniqueness
Mixture of substances, including resistant starch, vitamin, mineral, phytochemicalss and antioxidant content.
After feed fermentation, the content of small peptide substantially increases.The Main Function of small peptide is that absorption is fast, is difficult saturation and amino
Acid has independent mechanism of absorption, contribute to mitigating free amino acid vie each other it is common absorb the antagonism that site produces, also
The transhipment of free amino acid can be promoted, so as to promote Amino Acid Absorption to improve protein utilization rate.Small peptide has protection to digesting stage property
Effect, makes young animal small intestinal precocious, the secretion of the enzyme that stimulates digestion.Small peptide has immunocompetence, can make to have in animal alimentary canal
Bacteria group is bred, and improves body's resistance to disease.
Oat bran crude protein only has 4%, and crude fiber content reaches 29%, moisture 7.2%, ash 2.79, crude fibre
29.68%, it is a kind of extraordinary crude fibre raw material.But acid detergent fiber content is high, aflatoxin and vomitoxin
It is exceeded serious.
Oat bran is also containing abundant fiber and other nutritional labelings such as ferrum, potassium and vitamin B1 etc..Send out through microorganism
After ferment, crude protein content increase 50-60%, ferrum, potassium and vitamin B1 equal size increase 10-15%, mycotoxin of effectively degrading,
Contain multiple-microorganism metabolite simultaneously, growth of animal can be promoted, strengthen disease resistance.
Citrus pulp is to produce the product that the remaining pulp of canned fruit juice, pit, peel crushed after being dried are obtained with citrusfruit
Thing, color are in faint yellow or brown, have light Citrus taste, taste slightly bitterness, and 90% can be by 10 mesh standard sieves, and 100% can lead to
Cross 8 mesh standard sieves.In citrus pulp, general component content is：Dry 90.00%., wherein crude protein 9.8%, crude fat
3.7%, 30.6% crude fibre, nitrogen-free extract 60.3%, coarse ash 6.6%, calcium 1.84%, phosphorus 0.12%.In citrus pulp
Containing and have the material of bitterness, have impact on the effectively utilizes of animal.16 kinds of Bravo and dichlorvos residual etc. in citrus peel residue
Pesticide, the impact of residual content can effectively be degraded by fermentation, and improve the nutrient contents such as crude protein, be significantly reduced thick fibre
Dimension hplc.
Citrus pulp is 13.79MJ/kg to the digestible energy of ruminant, is 13.78MJ/kg to the digestible energy of pig, to chicken
Metabolizable energy is 6.27MJ/kg.Citrus pulp arginine content 0.27%, lysine 0.22%, methionine 0.10%, cystine
0.12%, tryptophan 0.07%, ferrum are 378.0mg/kg, and copper 6.0mg/kg, manganese are 7.0mg/kg, and zinc is 15mg/kg.Citrus pulp
Nutritive value have very big change by fermentation afterwards, compared with unfermentable dry powder, thick protein improve 50%, aminoacid is carried
High by 55%, crude fat improves 24%, and vitamin improves 10%-60%, and crude fibre declines 17%, illustrates that fermentation can overcome the disadvantages that dry powder egg
The deficiency of white matter, aminoacid, and increase vitamin content so as to nutrition reaches higher level, and ferment 16 kinds of aminoacid of citrus pulp
Content all increased total amino acidss content increases to 10.84% from 7.59% respectively；Essential amino acids content increases
49.72%；Non essential amino acid increases by 41.18% wherein lysine and increases by 80%；Methionine increases by 41.67%；Threonine increases
Plus 82.76%.To feeding cow, the Semen Maydis powder of 40%-45%, and milch cow fur light, premunition can be replaced to strengthen.
Herba Medicaginiss are a kind of very excellent vegetable protein feeds, and crude protein content is high, digestibility is high, rich in vitamin
With various mineral, more than 18%, feeding cow is remarkably improved the milk yield of milch cow to Herba Medicaginiss dry gross protein value
And milk quality, can also reduce because excessively eating the long milch cow utilization periods of milk that concentrate causes, increase economic efficiency.Research table
Bright, after undergoing microbial fermentation, Alfalfa crude fiber content is 11.90%, reduces 38.34%, crude protein during than not fermenting
Matter and true protein content are 29.62%, 22.97%, improve 42.41% and 28.97% when respectively than not fermenting, acid-soluble
The content of small peptide rises 12.59%, it is necessary to which aminoacid and total amino acidss content increased, and undergoing microbial fermentation to make lucerne
The gross protein value relative increase of Mu powder, reduces content of cellulose, improves acid-soluble peptide content, so as to improve the feeding of alfalfa meal
Use quality.
Alfalfa meal is the extraordinary cellulose source of nutrition of pig chicken feed, wherein containing crude protein more than 20%, it is thick fine
Dimension more than 21%, the organic matter digestibility of pig only have 47%, and containing the harmful bacterias such as certain escherichia coli and Aspergillus flavus poison
The harmful substances such as element, after fermentation, improve the quantity of probioticss, reduce the quantity of harmful bacteria, are effectively improved pig chicken intestinal
Health status.
Chinese invention application reduces the method that crude fibre produces feedstuff, application number with stalk fermentation：201410657067.2
A kind of utilization stalk fermentation of disclosure of the invention reduces the method that crude fibre produces feedstuff, is related to agricultural technology field；Raw material preparation,
Stirring, pack, material are collected, add carbamide, modulation bacterium solution, pack, ferment in second time, packaging etc.；The present invention can solve current straw
Stalk is not used as the low problem of feed stripped, straw effective rate of utilization.
Compound bacteria-fermented high fiber agricultural byproducts produce the method for animal probiotics feed by utilizing, application number：
201110237021.1 disclose a kind of method that compound bacteria-fermented high fiber agricultural byproducts produce animal probiotics feed by utilizing, be by
High microsteping agricultural byproducts remove impurity, pulverization process, with adjuvant (0～6.0% ammonium sulfate, and/or 0～3.0% potassium dihydrogen phosphate and/
Or 0～1.0% calcium chloride and/or 0～1.0% magnesium sulfate) mix in proportion after moisten water steaming；It is cooled to after the completion of steaming
20～40 DEG C, while the sturdy vein born of the same parents bacterium of inoculation and Lactobacillus plantarum, 2～5d of solid fermentation at 20～40 DEG C；Fermentation is completed
Feedstuff be placed in 40 DEG C～70 DEG C crushed after being dried, obtain final product the probiotic feed product rich in carotenoid.This method has life
Efficiency high is produced, low cost, profitable advantage, while solving problem of environmental pollution, alleviate energy again and albumen feedstuff comes
The pressure of source critical shortage.
The content of the invention
The raw material such as oat bran, citrus pulp and Alfalfa that the present invention is directed to crude fiber content is high, have mycotoxin contamination etc.
Situation, takes fermentation technique, increases the nutrient contents such as small peptide, reduces crude fiber content, increases the beneficial unknown factor, and improves
Probioticss quantity, improves utilizing status.During composite fibre by fermentation can use chicken feed in a large number, reduces cost it is same
When, safe feed guarantee is provided for chicken.
In order to realize above-mentioned target, the scheme that the present invention is adopted is：
A kind of mixed fermentation fiber feedstuff for laying hen feeding, is prepared by the raw material of following parts by weight：
Citrus pulp, oat bran, 1 part of the yeast mixed fermentation product A of Alfalfa, citrus pulp, oat bran, Alfalfa
Lactic acid bacteria mixed fermentation product B 1-3 parts, citrus pulp, oat bran, bacillus cereuss mixed fermentation product C 2-3 parts of Alfalfa.
The A is prepared by following methods：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1-2:1-3 is constituted
Raw mixture be added in heatable mixer, add the water of raw mixture quality 40-60%, the Fructus Vitis viniferae of 3-5%
Material, after mix homogeneously post-heating to 80-95 DEG C of 20-30 minute, is cooled to 25-30 DEG C by sugar, adds raw mixture quality
The mixed yeast seed liquor of 0.2-0.5%, cultivates 48-72 hours at 25-30 DEG C, and speed of agitator is 20-30r/min, culture
The Radix Glycyrrhizae powder particle of L-Cysteine, 0.05-0.1% to 30-40 hours addition raw mixture quality 0.005-0.1%,
Fluid bed drying is used after fermentation ends, and the yeast for moisture being obtained less than the citrus pulp of 14wt%, oat bran, Alfalfa is mixed
Close tunning A.
The B is prepared by following methods：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1-3.5:1-2.5
The raw mixture of composition is added in heatable mixer, adds water, the 5-10% Portugals of raw mixture quality 30-70%
Material was cooled to 25-30 DEG C after 30 minutes by grape sugar, mix homogeneously post-heating to 70-80 DEG C, addition raw mixture quality
The mixing lactic acid bacteria seed liquor of 0.5-2%, cultivates 48-72 hours at 30-37 DEG C, uses fluid bed drying, obtain after fermentation ends
Moisture is less than the citrus pulp of 14wt%, oat bran, the lactic acid bacteria mixed fermentation product B of Alfalfa.
The C is prepared by following methods：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1-2.5:1-4 groups
Into raw mixture be added in heatable mixer, add the water of raw mixture quality 50-60%, 2-3% Fructus Vitis viniferaes
Material was cooled to 25-30 DEG C after 30 minutes by sugar, mix homogeneously post-heating to 60-80 DEG C, added raw mixture quality 0.1-
The 0.3% sub- liquid of mixing spore bacillus specie, cultivates 60-90 hours at 25-30 DEG C, and speed of agitator is 30-50r/min.Fermentation knot
Shu Houyong fluid bed dryings, the bacillus cereuss mixing for obtaining moisture less than the citrus pulp of 14wt%, oat bran, Alfalfa are sent out
Ferment product C.
The mixed yeast seed liquor is prepared by following methods：By Candida utilis and saccharomyces cerevisiae point
It is not inoculated in PDY fluid mediums, at 25-32 DEG C, 150-200rpm culture 30-40 hours respectively obtain Candida utilis
Saccharomycete seed liquor, saccharomyces cerevisiae seed liquor, then by Candida utilis seed liquor, saccharomyces cerevisiae seed liquor according to body
Product compares 1:1～1:To in mixed yeast seed liquid culture medium, in 25-32 DEG C, 150-200rpm cultivates 20-30 for 5 combined inoculations
Hour, obtain mixed yeast seed liquor.The preparation method of mixed yeast seed liquid culture medium is as follows：Peptone 15.0g, Portugal
Grape sugar 18.0g, yeast extract 3.0g, Sodium Chloride 5.0g, distilled water 1000ml, pH value 6.8-7.2,121 DEG C sterilizing 20min.
The saccharomyces cerevisiae is CGMCC No.12789.
The mixing lactic acid bacteria seed liquor is prepared by following methods：Lactobacillus plantarum, pediococcus acidilactici are connect respectively
Plant in MRS fluid mediums, 20-30 hours are cultivated at 32-37 DEG C, Lactobacillus plantarum seed liquor, lactic acid sheet is respectively obtained
Coccus seed liquor, then by Lactobacillus plantarum seed liquor, pediococcus acidilactici seed liquor according to volume ratio 1:2～1:4 combined inoculations are arrived
In mixing lactic acid bacteria seed liquid culture medium, 20-30 hours are cultivated at 30-37 DEG C, mixing lactic acid bacteria seed liquor is obtained.Wherein mix
The compound method of lactobacillus solution culture medium is as follows：Peptone 12g, glucose 25g, Carnis Bovis seu Bubali cream 8g, yeast extract 6g, phosphorus
Sour disodium hydrogen 5g, Fructus Citri Limoniae acid diamine 0.25g, sodium acetate 6g, Tween 80 2ml, magnesium sulfate 0.8g, manganese sulfate 0.5g, ferrous sulfate
0.2g, distilled water 1000ml, pH value 6.0-6.5,120 DEG C of sterilizing 20min.
The mixing bacillus cereuss seed liquor is prepared by following methods：By bacillus subtilises, Bacillus licheniformis
It is inoculated in TSB fluid mediums respectively, 12-30 hours is cultivated under the conditions of 30-37 DEG C, 150-250rpm, is respectively obtained withered
Careless bacillus cereuss seed liquor, Bacillus licheniformis seed liquor, then by bacillus subtilises seed liquor, Bacillus licheniformis seed liquor
According to volume ratio 1:1～1:3 combined inoculations to mix bacillus cereuss seed liquid culture medium in, 30-37 DEG C, 150-250rpm culture
12-30 hours, obtain mixing bacillus cereuss seed liquor.Wherein mix the sub- liquid culture medium compound method of spore bacillus specie as follows：Greatly
Soybean protein peptone 10g, glucose 15g, tryptone 5g, dibastic sodium phosphate 4g, potassium chloride 2g, distilled water 1000ml, pH value 6.8-
7.0,120 DEG C of sterilizing 20min.
It is a further object of the present invention to provide a kind of preparation side of the above-mentioned mixed fermentation fiber feedstuff for laying hen feeding
Method.
In order to realize above-mentioned target, the scheme that the present invention is adopted is：
A kind of preparation method of the above-mentioned mixed fermentation fiber feedstuff for laying hen feeding, comprises the following steps：By Citrus
Slag, oat bran, the yeast mixed fermentation product A of Alfalfa, lactic acid bacteria mixed fermentation product B, bacillus cereuss mixed fermentation are produced
Thing C is according to mass ratio 1:1-3:2-3 mix homogeneously obtains citrus pulp, oat bran, Alfalfa mixed fermentation end-product.
Beneficial effect
Fermented by the mixing of citrus pulp, oat bran, Alfalfa, can in chicken feed as a kind of fermentable fiber feed applications
Developed and Crypt depth with the intestinal villus for significantly improving chicken, improve digestive function and intestinal health state；Mixing after fermentation is fine
Dimension crude protein content of feed improves more than 40%, and the content of acid-soluble small peptide improves more than 12%, increases unknown trophic factors etc.
Content, reduces crude fibre more than 30%, significantly improves the organic matter digestibility of feedstuff, improves immunologic function and premunition, to carry
Strong guarantee is provided for safe chicken product.Increase paddy in product after adding L-Cysteine during Yeast Cultivation
The content of the sweet peptide of Guang, and then increase the immunity nourishment function of product.
Specific embodiment
Below by the specific embodiment narration present invention.Unless stated otherwise, technological means used in the present invention
It is method known in those skilled in the art.In addition, embodiment be interpreted as it is illustrative, and it is unrestricted the present invention
Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this
On the premise of invention spirit and scope, various changes that the material component and consumption in these embodiments is carried out or change
Belong to protection scope of the present invention.
The following examples can make those skilled in the art that the present invention is more fully understood, but limit never in any form
The present invention.
CGMCC No.12789 are specially saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016, the bacterial strain
In being preserved in the China Committee for Culture Collection of Microorganisms's common micro-organisms center of on July 15th, 2016, deposit number is
CGMCC No.12789, preservation address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica,
Postcode 100101.
The most suitable growth pH of the saccharomyces cerevisiae tlj2016 is 6.0-6.5, and optimum growth temperature is 28-35 DEG C；
The saccharomyces cerevisiae tlj2016 is isolated below saccharomyces cerevisiae starting strain Jing from the orchard of one plant of Ningxia
Step is obtained：
The original strain that sets out → test tube activation → dithyl sulfate (DES) mutation → hypertonic plate screening → nitrosoguanidine
(NTG) secondary screening (producing GSH abilities) → mitotic stability test is screened → fermented to mutagenesis screening → hypertonic flat board primary dcreening operation → shaking flask.
1st, saccharomyces cerevisiae provided by the present invention reaches 300g/L to the tolerance of glucose, beneficial to which in high concentration Portugal
GSH is produced under the conditions of grape sugar；
2nd, saccharomyces cerevisiae provided by the present invention reaches 3308mg/L in 5L fermentation cylinder for fermentation production GSH final concentrations；
3rd, the ability of saccharomyces cerevisiae tolerance L-Cysteine provided by the present invention is far above starting strain, in 5mmol/L
Slow growth is remained under L-Cysteine effect, remains to keep GSH to synthesize in a large number under the effect of 40mmol/LL- cysteine；
4th, saccharomyces cerevisiae salt resistance ability provided by the present invention reaches 18%, is conducive to extending its application.
(1) under the conditions of high sugar, GSH capacity experimentals are produced in tlj2016 fermentations
(1) shake-flask culture
One ring of tlj2016 slant strains is taken, is accessed and 150rpm in the 250mL shaking flasks of 30mL Shake flask mediums is housed, 30 DEG C
Culture 30h obtains seed liquor；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 35, K2HPO4·3H2O 3、KH2PO40.5th, yeast powder 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
(2) 5L fermentor cultivation
Seed liquor is pressed into 10% inoculum concentration, is accessed in the fermentation tank equipped with 3L fermentation medium, 30 DEG C, ventilation 6L/
Min, tank pressure 0.03MPa, 500rpm carry out fermentation culture under the conditions of permanent pH6.0, when fermenting to 30h, disposably add final concentration
For the L-Cysteine of 25mmol/L, total fermentation time is 50h；
Fermentation medium (g/L)：(NH4)2SO410th, glucose 100, K2HPO4·3H2O 8、KH2PO40.5th, yeast powder
11、MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1,pH6.0；
After fermentation ends, the content for determining GSH in fermentation liquid is 3308mg/L.
(2) L-Cysteine tolerance experiment
By starting strain and each ring of tlj2016 slant strains, the 250mL equipped with 30mL Shake flask mediums is respectively connected to
150rpm in shaking flask, 30 DEG C are cultivated, and the half Guang ammonia of L- of different final concentrations when culture is to 12h, is added in shaking flask
Acid, is further cultured for 10h, determines dry cell weight, as a result table 1,2；
Shake flask medium (g/L)：(NH4)2SO46th, glucose 20, K2HPO4·3H2O 3、KH2PO40.5th, yeast powder 11,
MnSO4 0.1、KCL 0.1、FeSO4 0.1、MgSO4·7H2O 0.1, pH6.0；
Table 1：Starting strain L-Cysteine tolerance
| L-Cysteine concentration mmol/L | 0 | 5 | 10 | 15 | 20 | 40 |
| Starting strain dry weight g/L | 22.6 | 15.7 | 10.2 | 4.3 | 2.2 | 0.8 |
| GSH concentration (mg/L) | 35.6 | 46.7 | 43.2 | 40.7 | 37.9 | 25.3 |
Table 2：Tlj2016L- cysteine tolerances
| L-Cysteine concentration mmol/L | 0 | 5 | 10 | 15 | 20 | 40 |
| Tlj2016 dry weights g/L | 25.7 | 28.5 | 23.6 | 21.2 | 20.6 | 18.7 |
| GSH concentration (mg/L) | 73.2 | 98.3 | 113.5 | 121.7 | 127.5 | 135.8 |
From the results shown in Table 2, for starting strain, in culture medium, add L-Cysteine, cell stops growing,
And start self-dissolving, cause GSH rate of increase to reduce with the rising of L-Cysteine concentration；And low concentration L-Cysteine
Under, tlj2016 still is able to slow growth, with the raising of L-Cysteine concentration, under the dry cell weight of tlj2016 bacterial strains is slow
Drop, and GSH concentration sustainable growths, this result pass through to add half Guang ammonia of precusor amino acids-L- in being beneficial to GSH production processes
Acid proposes the production for promoting GSH.
(3) salt resistance ability experiment
Take tlj2016 bacterium solutions 1mL inoculation strain in containing different NaCl concentrations (concentration gradients are 0%, 2%, 5%,
10%th, 15%, 10mL YPD fluid mediums (pH=6.5) 18%), is placed at 30 DEG C and cultivates 24h respectively, and each processes 3
Individual repetition.Respectively take 1ml samples bacterium solution to mix in 9ml normal saline, prepare dilution factor solution, take 0.1ml diluents solid in YPD
It is coated with body flat board, culture 36 hours (each dilution factor do 3 parallel) record is inverted in 30 DEG C of biochemical cultivation cases and calculates flat
Bacterium number number on plate.The results are shown in Table 3, it is known that the resistance to salinity of the bacterium is 18%, illustrates that tlj2016 not only can be in conventional ring
Survive in border, still there is under high salt conditions vigor, can be applicable to consumption sugar in the high salt food processing process such as soy sauce, curing food
Produce glutathion.
Table 3：Salt resistance ability detection (× 107cfu/ml)
Embodiment 1：
A kind of mixed fermentation fiber feedstuff for laying hen feeding, is prepared by following methods：
By citrus pulp, oat bran, the yeast mixed fermentation product A of Alfalfa, lactic acid bacteria mixed fermentation product B, spore
Bacillus mixed fermentation product C is according to mass ratio 1:2:3 mix homogeneously obtain mixed fermentation end-product product.Paddy Guang in feed product
The content of sweet peptide is 36mg/kg.
The preparation of A：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1:The raw mixture of 3 compositions is added to can
In the mixer of heating, water, 4% glucose of raw mixture quality 50%, mix homogeneously post-heating to 90 DEG C 30 points are added
Zhong Hou, material is cooled to after 27 DEG C, adds the mixed yeast seed liquor of raw mixture quality 0.3%, is cultivated at 27 DEG C
56 hours, speed of agitator was 25r/min, the L-Cysteine of culture to 36 hours addition raw mixture quality 0.008%,
0.08% Radix Glycyrrhizae powder particle, uses fluid bed drying after fermentation ends, it is the citrus pulp of 14wt%, Herba bromi japonici to obtain moisture
The yeast mixed fermentation product A of bran, Alfalfa.
The preparation of B：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:2:The raw mixture of 2 compositions is added to can
In the mixer of heating, water, 8% glucose of raw mixture quality 60%, mix homogeneously post-heating to 75 DEG C 30 points are added
Material is cooled to 27 DEG C by Zhong Hou, adds the mixing lactic acid bacteria seed liquor of raw mixture quality 1%, little in 33 DEG C of cultures 56
When, fluid bed drying is used after fermentation ends, citrus pulp, oat bran, the lactic acid bacteria of Alfalfa that moisture is 14wt% is obtained
Mixed fermentation product B.
The preparation of C：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:2:The raw mixture of 3 compositions is added to can
In the mixer of heating, water, 2% glucose of raw mixture quality 50%, mix homogeneously post-heating to 70 DEG C 30 are added
After minute, material is cooled to into 27 DEG C, adds the sub- liquid of mixing spore bacillus specie of raw mixture quality 0.2%, trained at 27 DEG C
Support 70 hours, speed of agitator is 40r/min.Fluid bed drying is used after fermentation ends, the Citrus that moisture is 14wt% are obtained
Slag, oat bran, the bacillus cereuss mixed fermentation product C of Alfalfa.
The preparation of mixed yeast seed liquor：Candida utilis, saccharomyces cerevisiae are inoculated in into the training of PDY liquid respectively
In foster base, at 28 DEG C, 150rpm is cultivated 35 hours, respectively obtains protein candidiasis seed liquid, saccharomyces cerevisiae seed liquor,
Again by Candida utilis seed liquor, saccharomyces cerevisiae seed liquor according to volume ratio 1:3 combined inoculations are to compound barm strain
In sub- liquid culture medium, in 26 DEG C, 150rpm is cultivated 22 hours, obtains mixed yeast seed liquor.Mixed yeast seed liquor is trained
The preparation method of foster base is as follows：Peptone 15.0g, glucose 18.0g, yeast extract 3.0g, Sodium Chloride 5.0g, distilled water
1000ml, 6.8,121 DEG C of sterilizing 20min of pH value.The saccharomyces cerevisiae is CGMCC No.12789, and Candida utilis are
CICC1268。
The preparation of mixing lactic acid bacteria seed liquor：Lactobacillus plantarum, pediococcus acidilactici are inoculated in into MRS fluid mediums respectively
In, cultivate 25 hours at 35 DEG C, respectively obtain Lactobacillus plantarum seed liquor, pediococcus acidilactici seed liquor, then by plant breast bar
Bacterium seed liquor, pediococcus acidilactici seed liquor are according to volume ratio 1:3 combined inoculations in mixing lactic acid bacteria seed liquid culture medium, 33 DEG C
Lower culture 27 hours, obtains mixing lactic acid bacteria seed liquor.The compound method of wherein mixing lactic acid bacteria seed liquid culture medium is as follows：Egg
White peptone 12g, glucose 25g, Carnis Bovis seu Bubali cream 8g, yeast extract 6g, disodium hydrogen phosphate 5g, Fructus Citri Limoniae acid diamine 0.25g, sodium acetate
6g, Tween 80 2ml, magnesium sulfate 0.8g, manganese sulfate 0.5g, ferrous sulfate 0.2g, distilled water 1000ml, 6.0,120 DEG C of pH value go out
Bacterium 20min.The Lactobacillus plantarum is CICC20022, and pediococcus acidilactici is CICC10344.
Mix the preparation of bacillus cereuss seed liquor：Bacillus subtilises, Bacillus licheniformis are inoculated in into TSB liquid respectively
In culture medium, cultivate 18 hours under the conditions of 32 DEG C, 200rpm, respectively obtain bacillus subtilises seed liquor, lichens spore bar
Bacterium seed liquor, then by bacillus subtilises seed liquor, Bacillus licheniformis seed liquor according to volume ratio 1:2 combined inoculations are to mixing
In bacillus cereuss seed liquid culture medium, 32 DEG C, 200rpm cultivate 18 hours, obtain mix bacillus cereuss seed liquor.Wherein mix
Bacillus cereuss seed liquid culture medium compound method is as follows：Soy peptone 10g, glucose 15g, tryptone 5g, dibastic sodium phosphate
4g, potassium chloride 2g, distilled water 1000ml, 6.8,120 DEG C of sterilizing 20min of pH value.The bacillus subtilises are CICC10732,
Bacillus licheniformis CGMCC1884.
Embodiment 2：
A kind of mixed fermentation fiber feedstuff for laying hen feeding, is prepared by following methods：
By citrus pulp, oat bran, the yeast mixed fermentation product A of Alfalfa, lactic acid bacteria mixed fermentation product B, spore
Bacillus mixed fermentation product C is according to mass ratio 1:1:2 mix homogeneously obtain citrus pulp, oat bran, Alfalfa mixed fermentation product eventually
Thing；The content of feed product GSH-PX activity is 38mg/kg.
The preparation of A：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:2:The raw mixture of 1 composition is added to can
In the mixer of heating, water, 3% glucose of raw mixture quality 40%, mix homogeneously post-heating to 80 DEG C 20 points are added
Material is cooled to 25 DEG C by Zhong Hou, adds the mixed yeast seed liquor of raw mixture quality 0.2%, cultivates 48 at 25 DEG C
Hour, speed of agitator is 20r/min, the L-Cysteine of culture to 30 hours addition raw mixture quality 0.005%,
0.1% Radix Glycyrrhizae powder particle, uses fluid bed drying after fermentation ends, obtain moisture be the citrus pulp of 13wt%, oat bran,
The yeast mixed fermentation product A of Alfalfa.
The preparation of B：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1:The raw mixture of 1 composition is added to can
In the mixer of heating, water, 5% glucose of raw mixture quality 30%, mix homogeneously post-heating to 80 DEG C 30 points are added
Material is cooled to 25 DEG C by Zhong Hou, adds the mixing lactic acid bacteria seed liquor of raw mixture quality 0.5%, cultivates 48 at 30 DEG C
Hour, fluid bed drying is used after fermentation ends, citrus pulp, oat bran, the lactic acid of Alfalfa that moisture is 13wt% is obtained
Bacterium mixed fermentation product B.
The preparation of C：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:2.5:The raw mixture of 1 composition is added to
In heatable mixer, water, 3% glucose of raw mixture quality 60%, mix homogeneously post-heating to 80 DEG C 30 are added
After minute, material is cooled to into 25 DEG C, adds the sub- liquid of mixing spore bacillus specie of raw mixture quality 0.1%, trained at 25 DEG C
Support 60 hours, speed of agitator is 30r/min.Fluid bed drying is used after fermentation ends, the Citrus that moisture is 13wt% are obtained
Slag, oat bran, the bacillus cereuss mixed fermentation product C of Alfalfa.
The preparation of mixed yeast seed liquor：Candida utilis, saccharomyces cerevisiae are inoculated in into the training of PDY liquid respectively
In foster base, at 25 DEG C, 200rpm is cultivated 30 hours, respectively obtains Candida utilis seed liquor, saccharomyces cerevisiae seed
Liquid, then by Candida utilis seed liquor, saccharomyces cerevisiae seed liquor according to volume ratio 1:1 combined inoculation is to mixed yeast
In seed liquid culture medium, in 25 DEG C, 200rpm is cultivated 20 hours, obtains mixed yeast seed liquor.Mixed yeast seed liquor
The preparation method of culture medium is as follows：Peptone 15.0g, glucose 18.0g, yeast extract 3.0g, Sodium Chloride 5.0g, distillation
Water 1000ml, 7.2,121 DEG C of sterilizing 20min of pH value.The saccharomyces cerevisiae is CGMCC No.12789, and Candida utilis are
CICC1314。
The preparation of mixing lactic acid bacteria seed liquor：Lactobacillus plantarum, pediococcus acidilactici are inoculated in into MRS fluid mediums respectively
In, cultivate 20 hours at 32 DEG C, respectively obtain Lactobacillus plantarum seed liquor, pediococcus acidilactici seed liquor, then by plant breast bar
Bacterium seed liquor, pediococcus acidilactici seed liquor are according to volume ratio 1:2 combined inoculations in mixing lactic acid bacteria seed liquid culture medium, 30 DEG C
Lower culture 20 hours, obtains mixing lactic acid bacteria seed liquor.The compound method of wherein mixing lactic acid bacteria seed liquid culture medium is as follows：Egg
White peptone 12g, glucose 25g, Carnis Bovis seu Bubali cream 8g, yeast extract 6g, disodium hydrogen phosphate 5g, Fructus Citri Limoniae acid diamine 0.25g, sodium acetate
6g, Tween 80 2ml, magnesium sulfate 0.8g, manganese sulfate 0.5g, ferrous sulfate 0.2g, distilled water 1000ml, 6.5,120 DEG C of pH value go out
Bacterium 20min.The Lactobacillus plantarum is CICC20038, pediococcus acidilactici CICC10146.
Mix the preparation of bacillus cereuss seed liquor：Bacillus subtilises, Bacillus licheniformis are inoculated in into TSB liquid respectively
In culture medium, cultivate 30 hours under the conditions of 30 DEG C, 150rpm, respectively obtain bacillus subtilises seed liquor, lichens spore bar
Bacterium seed liquor, then by bacillus subtilises seed liquor, Bacillus licheniformis seed liquor according to volume ratio 1:1 combined inoculation is to mixing
In bacillus cereuss seed liquid culture medium, 30 DEG C, 150rpm cultivate 30 hours, obtain mix bacillus cereuss seed liquor.Wherein mix
Bacillus cereuss seed liquid culture medium compound method is as follows：Soy peptone 10g, glucose 15g, tryptone 5g, dibastic sodium phosphate
4g, potassium chloride 2g, distilled water 1000ml, 7.0,120 DEG C of sterilizing 20min of pH value.The bacillus subtilises are CICC10732,
Bacillus licheniformis are CGMCC1884.
Embodiment 3
A kind of mixed fermentation fiber feedstuff for laying hen feeding, has following methods to prepare：
By citrus pulp, oat bran, the yeast mixed fermentation product A of Alfalfa, lactic acid bacteria mixed fermentation product B, spore
Bacillus mixed fermentation product C is according to mass ratio 1:3:2 mix homogeneously obtain citrus pulp, oat bran, Alfalfa mixed fermentation product eventually
Thing.The content of feed product GSH-PX activity is 37mg/kg.
The preparation of A：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1:The raw mixture of 3 compositions is added to can
In the mixer of heating, water, 5% glucose of raw mixture quality 60%, mix homogeneously post-heating to 95 DEG C 30 are added
After minute, material is cooled to into 30 DEG C, adds the mixed yeast seed liquor of raw mixture quality 0.5%, cultivated at 30 DEG C
72 hours, speed of agitator was 30r/min, the L-Cysteine of culture to 40 hours addition raw mixture quality 0.1%,
0.05% Radix Glycyrrhizae powder particle, uses fluid bed drying after fermentation ends, it is the citrus pulp of 12wt%, Herba bromi japonici to obtain moisture
The yeast mixed fermentation product A of bran, Alfalfa.
The preparation of B：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:3.5:The raw mixture addition of 2.5 compositions
To in heatable mixer, water, 10% glucose of raw mixture quality 70%, mix homogeneously post-heating to 70 are added
DEG C after 30 minutes, material is cooled to into 30 DEG C, adds the mixing lactic acid bacteria seed liquor of raw mixture quality 2%, trained at 37 DEG C
Support 72 hours, after fermentation ends, use fluid bed drying, obtain citrus pulp, oat bran, Alfalfa that moisture is 12wt%
Lactic acid bacteria mixed fermentation product B.
The preparation of C：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1:The raw mixture of 4 compositions is added to can
In the mixer of heating, water, 2% glucose of raw mixture quality 50%, mix homogeneously post-heating to 60 DEG C 30 are added
After minute, material is cooled to into 30 DEG C, adds the sub- liquid of mixing spore bacillus specie of raw mixture quality 0.3%, trained at 30 DEG C
Support 90 hours, speed of agitator is 50r/min.Fluid bed drying is used after fermentation ends, the Citrus that moisture is 12wt% are obtained
Slag, oat bran, the bacillus cereuss mixed fermentation product C of Alfalfa.
The preparation of mixed yeast seed liquor：Candida utilis, saccharomyces cerevisiae are inoculated in into the training of PDY liquid respectively
In foster base, at 32 DEG C, 200rpm is cultivated 40 hours, respectively obtains Candida utilis seed liquor, saccharomyces cerevisiae seed
Liquid, then by Candida utilis seed liquor, saccharomyces cerevisiae seed liquor according to volume ratio 1:5 combined inoculations are to mixed yeast
In seed liquid culture medium, in 32 DEG C, 150rpm is cultivated 30 hours, obtains mixed yeast seed liquor.Mixed yeast seed liquor
The preparation method of culture medium is as follows：Peptone 15.0g, glucose 18.0g, yeast extract 3.0g, Sodium Chloride 5.0g, distillation
Water 1000ml, 7,121 DEG C of sterilizing 20min of pH value.The saccharomyces cerevisiae is CGMCC No.12789, and Candida utilis are
CICC1314。
The preparation of mixing lactic acid bacteria seed liquor：Lactobacillus plantarum, pediococcus acidilactici are inoculated in into MRS fluid mediums respectively
In, cultivate 30 hours at 37 DEG C, respectively obtain Lactobacillus plantarum seed liquor, pediococcus acidilactici seed liquor, then by plant breast bar
Bacterium seed liquor, pediococcus acidilactici seed liquor are according to volume ratio 1:4 combined inoculations in mixing lactic acid bacteria seed liquid culture medium, 37 DEG C
Lower culture 30 hours, obtains mixing lactic acid bacteria seed liquor.The compound method of wherein mixing lactic acid bacteria seed liquid culture medium is as follows：Egg
White peptone 12g, glucose 25g, Carnis Bovis seu Bubali cream 8g, yeast extract 6g, disodium hydrogen phosphate 5g, Fructus Citri Limoniae acid diamine 0.25g, sodium acetate
6g, Tween 80 2ml, magnesium sulfate 0.8g, manganese sulfate 0.5g, ferrous sulfate 0.2g, distilled water 1000ml, 6.2,120 DEG C of pH value go out
Bacterium 20min.The Lactobacillus plantarum is CICC20038, pediococcus acidilactici CICC10146.
Mix the preparation of bacillus cereuss seed liquor：Bacillus subtilises, Bacillus licheniformis are inoculated in into TSB liquid respectively
In culture medium, cultivate 12 hours under the conditions of 37 DEG C, 250rpm, respectively obtain bacillus subtilises seed liquor, lichens spore bar
Bacterium seed liquor, then by bacillus subtilises seed liquor, Bacillus licheniformis seed liquor according to volume ratio 1:3 combined inoculations are to mixing
In bacillus cereuss seed liquid culture medium, 37 DEG C, 250rpm cultivate 12 hours, obtain mix bacillus cereuss seed liquor.Wherein mix
Bacillus cereuss seed liquid culture medium compound method is as follows：Soy peptone 10g, glucose 15g, tryptone 5g, dibastic sodium phosphate
4g, potassium chloride 2g, distilled water 1000ml, 7.0,120 DEG C of sterilizing 20min of pH value.The bacillus subtilises are CICC10732,
Bacillus licheniformis are CGMCC1884.
Embodiment 4
Using effect test example
By feedstuff, common feedstuff obtained in the present embodiment 1, the test of 6 months of comprehensive laying hen is done.
1 test material
1.1 test period
Test in -2015 years on the 9th October 29 of August in 2014, test period 60 days.
1.2 test site
Test is implemented in Huan Renxian Xinhua of Liaoning Province field experiment.
Matched group is the peak material of laying eggs that equal proportion is fermentation raw material, and test group is obtained in the feeding embodiment of the present invention 1
Mixed fermentation fiber feedstuff.Learn through substantial amounts of gradient test result, mixed fermentation fiber feedstuff applies 15% on laying hen
Effect is best, therefore the result of the test of group profile of the present invention is addition 15%.
Test chicken is grouped：Test is designed using single factor experiment, and experiment material selects 1152 extra large blue brown 221 age in days eggs
Chicken, is divided into 2 groups, and 6 repetitions are set per group, and each repeats 96 chickens；
Difference fed control group and feedstuff group of the present invention.
Feeding method：Free choice feeding；
Test temperature：Using longitudinal ventilation, hen house temperature is controlled at 20-23 DEG C.Strictly control according to the blue brown feeding and management in sea
System；
Testing index includes：Beginning and end body weight, feed intake, survival rate.Laying rate, egg size, feedstuff-egg ratio, egg yolk face
Color, not unit and quality of egg are breathed out, the results are shown in Table 4.
Table 4：Impact of the present invention to laying cycle of laying hens egg laying performance
Note：Female different expressions significant difference (P ＜ 0.05) of colleague's shoulder marking-up.
As can be seen from the above results, the present invention affects notable to laying rate, improves 1.97%, has one to be fixed to egg size
Ring and improve 0.63%, but difference is not notable；Commodity egg rate improves 1.78%, and feedstuff-egg ratio reduces 0.34%；Death rate is reduced
2.41%；As can be seen here, mixed fermentation fiber feedstuff is significantly improved to Layer Production Performance, intestinal health, and death rate substantially drops
Low, economic benefit is significantly improved.
Present invention group fermentable fiber Feed Energy significantly reduces coliform count and Salmonella quantity in intestinal, makes intestinal
Microorganism re-forms the microecological balance and maintenance normal physiological function for being conducive to animal body；Probioticss lactic acid bacteria etc. can be with competing
Striving property repels harmful bacteria, prevents pathogen positioning transfer, and facilitating digestion road is repaired；Probioticss lactic acid bacteria that fermented feed is produced etc.
Lactic acid, volatile fatty acid and protein and peptide antagonistic substance can be produced, so as to suppress the harmful microbes such as escherichia coli to give birth to
It is long to breed and antagonism is produced to invasive organism；Various digestive enzyme are secreted, the utilization rate of feedstuff is improved；Can promote
Gut-associated lymphoid tissue develops, and contributes to the reaction " SBR " for maintaining these lymphoid tissues in height, improves antibody
Level, improves splenic T, the ratio of bone-marrow-derived lymphocyte, and T, the increasing number of bone-marrow-derived lymphocyte reduce SAC, improve dynamic
The humoral and cellular immune response level of object.
Claims (7)
1. it is a kind of for laying hen feeding mixed fermentation fiber feedstuff, it is characterised in that by following parts by weight raw material prepare：
Citrus pulp, oat bran, 1 part of the yeast mixed fermentation product A of Alfalfa, citrus pulp, oat bran, the yeast of Alfalfa
Bacterium mixed fermentation product lactic acid bacteria mixed fermentation product B 1-3 parts, citrus pulp, oat bran, the bacillus cereuss mixing of Alfalfa are sent out
Ferment product C 2-3 parts.
2. it is according to claim 1 it is a kind of for laying hen feeding mixed fermentation fiber feedstuff, it is characterised in that the A
Prepared by following methods：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1-2:The raw mixture of 1-3 compositions
It is added in heatable mixer, adds water, the glucose of 3-5% of raw mixture quality 40-60%, after mix homogeneously
After being heated to 80-95 DEG C of 20-30 minute, material is cooled to into 25-30 DEG C, adds the mixed of raw mixture quality 0.2-0.5%
Saccharomycete seed liquor being closed, 48-72 hours being cultivated at 25-30 DEG C, speed of agitator is 20-30r/min, culture adds to 30-40 hours
Plus L-Cysteine, the Radix Glycyrrhizae powder particle of 0.05-0.1% of raw mixture quality 0.005-0.1%, with stream after fermentation ends
Change bed to be dried, moisture is obtained less than the citrus pulp of 14wt%, oat bran, the yeast mixed fermentation product A of Alfalfa.
3. it is according to claim 1 it is a kind of for laying hen feeding mixed fermentation fiber feedstuff, it is characterised in that the B
Prepared by following methods：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1-3.5:The raw material of 1-2.5 compositions is mixed
Compound is added in heatable mixer, adds water, the glucose of 5-10% of raw mixture quality 30-70%, mixing
Material was cooled to 25-30 DEG C, adds mixing for raw mixture quality 0.5-2% after 30 minutes by uniform post-heating to 70-80 DEG C
Lactobacillus solution is closed, 48-72 hours is cultivated at 30-37 DEG C, use fluid bed drying, obtain moisture and be less than after fermentation ends
The citrus pulp of 14wt%, oat bran, the lactic acid bacteria mixed fermentation product B of Alfalfa.
4. it is according to claim 1 it is a kind of for laying hen feeding mixed fermentation fiber feedstuff, it is characterised in that the C
Prepared by following methods：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1-2.5:The raw material mixing of 1-4 compositions
Thing is added in heatable mixer, adds water, the 2-3% glucoses of raw mixture quality 50-60%, after mix homogeneously
60-80 DEG C is heated to after 30 minutes, material is cooled to into 25-30 DEG C, add the mixed bud of raw mixture quality 0.1-0.3%
Spore bacillus seed liquor, cultivates 60-90 hours at 25-30 DEG C, and speed of agitator is 30-50r/min；It is dry with fluid bed after fermentation ends
It is dry, moisture is obtained less than the citrus pulp of 14wt%, oat bran, the bacillus cereuss mixed fermentation product C of Alfalfa.
5. a kind of mixed fermentation fiber feedstuff for laying hen feeding according to claim 2, it is characterised in that described mixed
Close saccharomycete seed liquor to be prepared by following methods：Candida utilis, saccharomyces cerevisiae are inoculated in into PDY liquid respectively
In culture medium, at 25-32 DEG C, 150-200rpm culture 30-40 hours respectively obtain Candida utilis seed liquor, make
Brewer yeast bacterium seed liquor, then by Candida utilis seed liquor, saccharomyces cerevisiae seed liquor according to volume ratio 1:1～1:5 mix
Conjunction is inoculated in mixed yeast seed liquid culture medium, and in 25-32 DEG C, 150-200rpm culture 20-30 hours obtain mixing ferment
Female bacterium seed liquor；
The mixed yeast seed liquid culture medium is prepared by following methods：Peptone 15.0g, glucose 18.0g, yeast
Extract 3.0g, Sodium Chloride 5.0g, distilled water 1000ml, pH value 6.8-7.2,121 DEG C sterilizing 20min；
The saccharomyces cerevisiae is CGMCC No.12789.
6. the preparation method according to a kind of arbitrary described mixed fermentation fiber feedstuff for laying hen feeding of claim 1-5,
Comprise the steps：By citrus pulp, oat bran, the yeast mixed fermentation product A of Alfalfa, lactic acid bacteria mixed fermentation product B,
Bacillus cereuss mixed fermentation product C is according to 1:1-3:The ratio mix homogeneously of 2-3 obtains the mixed fermentation for laying hen feeding
Fiber feedstuff；
The preparation of the A：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1-2:The raw mixture addition of 1-3 compositions
To in heatable mixer, water, the glucose of 3-5% of raw mixture quality 40-60%, mix homogeneously post-heating are added
To after 80-95 DEG C of 20-30 minute, material is cooled to into 25-30 DEG C, adds the mixing ferment of raw mixture quality 0.2-0.5%
Female bacterium seed liquor, cultivates 48-72 hours at 25-30 DEG C, and speed of agitator is 20-30r/min, and culture is former to the addition of 30-40 hours
The L-Cysteine of material mixture quality 0.005-0.1%, the Radix Glycyrrhizae powder particle of 0.05-0.1%, use fluid bed after fermentation ends
Be dried, moisture is obtained less than the citrus pulp of 14wt%, oat bran, the yeast mixed fermentation product A of Alfalfa；
The preparation of the B：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1-3.5:1-2.5 the raw mixture of composition
It is added in heatable mixer, adds water, the 5-10% glucoses of raw mixture quality 30-70%, after mix homogeneously
70-80 DEG C is heated to after 30 minutes, material is cooled to into 25-30 DEG C, add the mixed lactic of raw mixture quality 0.5-2%
Bacterium seed liquor, cultivates 48-72 hours at 30-37 DEG C, and fluid bed drying is used after fermentation ends, obtains moisture less than 14wt%
Citrus pulp, oat bran, the lactic acid bacteria mixed fermentation product B of Alfalfa；
The C is prepared by following methods：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1-2.5:1-4 compositions
Raw mixture is added in heatable mixer, adds water, the 2-3% glucoses of raw mixture quality 50-60%, mixes
Uniform post-heating to 60-80 DEG C is closed after 30 minutes, material is cooled to into 25-30 DEG C, add raw mixture quality 0.1-0.3%
The sub- liquid of mixing spore bacillus specie, 25-30 DEG C cultivate 60-90 hours, speed of agitator is 30-50r/min；Use after fermentation ends
Fluid bed drying, obtains moisture less than the citrus pulp of 14wt%, oat bran, the bacillus cereuss mixed fermentation product of Alfalfa
C；
The preparation of the mixed yeast seed liquor：Candida utilis and saccharomyces cerevisiae are inoculated in into PDY liquid respectively
In culture medium, at 25-32 DEG C, 150-200rpm culture 30-40 hours respectively obtain Candida utilis seed liquor, make
Brewer yeast bacterium seed liquor, then by Candida utilis seed liquor, saccharomyces cerevisiae seed liquor according to volume ratio 1:1～1:5 mix
Conjunction is inoculated in mixed yeast seed liquid culture medium, and in 25-32 DEG C, 150-200rpm culture 20-30 hours obtain mixing ferment
Female bacterium seed liquor；
The preparation of the mixed yeast seed liquid culture medium：Peptone 15.0g, glucose 18.0g, yeast extract 3.0g,
Sodium Chloride 5.0g, distilled water 1000ml, pH value 6.8-7.2,121 DEG C sterilizing 20min.
The saccharomyces cerevisiae is CGMCC No.12789.
7. it is according to claim 6 it is a kind of for laying hen feeding mixed fermentation fiber feedstuff preparation method, including such as
Lower step：By citrus pulp, oat bran, the yeast mixed fermentation product A of Alfalfa, lactic acid bacteria mixed fermentation product B, spore bar
Bacterium mixed fermentation product C is according to 1:2:3 ratio mix homogeneously obtains mixed fermentation end-product product；
The preparation of the A：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:1:The raw mixture of 3 compositions is added to can
In the mixer of heating, water, 4% glucose of raw mixture quality 50%, mix homogeneously post-heating to 90 DEG C 30 points are added
Zhong Hou, material is cooled to after 27 DEG C, adds the mixed yeast seed liquor of raw mixture quality 0.3%, is cultivated at 27 DEG C
56 hours, speed of agitator was 25r/min, the L-Cysteine of culture to 36 hours addition raw mixture quality 0.008%,
0.08% Radix Glycyrrhizae powder particle, uses fluid bed drying after fermentation ends, it is the citrus pulp of 14wt%, Herba bromi japonici to obtain moisture
The yeast mixed fermentation product A of bran, Alfalfa；
The preparation of the B：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:2:The raw mixture of 2 compositions is added to can
In the mixer of heating, water, 8% glucose of raw mixture quality 60%, mix homogeneously post-heating to 75 DEG C 30 points are added
Material is cooled to 27 DEG C by Zhong Hou, adds the mixing lactic acid bacteria seed liquor of raw mixture quality 1%, little in 33 DEG C of cultures 56
When, fluid bed drying is used after fermentation ends, citrus pulp, oat bran, the lactic acid bacteria of Alfalfa that moisture is 14wt% is obtained
Mixed fermentation product B；
The preparation of the C：By citrus pulp, oat bran, Alfalfa according to mass ratio 1:2:The raw mixture of 3 compositions is added to can
In the mixer of heating, water, 2% glucose of raw mixture quality 50%, mix homogeneously post-heating to 70 DEG C 30 are added
After minute, material is cooled to into 27 DEG C, adds the sub- liquid of mixing spore bacillus specie of raw mixture quality 0.2%, trained at 27 DEG C
Support 70 hours, speed of agitator is 40r/min；Fluid bed drying is used after fermentation ends, the Citrus that moisture is 14wt% are obtained
Slag, oat bran, the bacillus cereuss mixed fermentation product C of Alfalfa；
The preparation method of the mixed yeast seed liquor：Candida utilis, saccharomyces cerevisiae are inoculated in into PDY liquid respectively
In body culture medium, at 28 DEG C, 150rpm is cultivated 35 hours, respectively obtains protein candidiasis seed liquid, saccharomyces cerevisiae strain
Sub- liquid, then by Candida utilis seed liquor, saccharomyces cerevisiae seed liquor according to volume ratio 1:3 combined inoculations are to compound barm
In the sub- liquid culture medium of strain, in 26 DEG C, 150rpm is cultivated 22 hours, obtains mixed yeast seed liquor；
The preparation of the mixed yeast seed liquid culture medium：Peptone 15.0g, glucose 18.0g, yeast extract 3.0g,
Sodium Chloride 5.0g, distilled water 1000ml, 6.8,121 DEG C of pH value sterilizing 20min；
The saccharomyces cerevisiae is CGMCC No.12789, and Candida utilis are CICC1268.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611078965.8A CN106551168A (en) | 2016-11-30 | 2016-11-30 | A kind of mixed fermentation fiber feedstuff for laying hen feeding and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611078965.8A CN106551168A (en) | 2016-11-30 | 2016-11-30 | A kind of mixed fermentation fiber feedstuff for laying hen feeding and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106551168A true CN106551168A (en) | 2017-04-05 |
Family
ID=58445906
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611078965.8A Pending CN106551168A (en) | 2016-11-30 | 2016-11-30 | A kind of mixed fermentation fiber feedstuff for laying hen feeding and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106551168A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109793097A (en) * | 2018-12-26 | 2019-05-24 | 河南巨龙生物工程股份有限公司 | A kind of fermentation process using sugaring tailing and tryptophan mother liquor production biological feedstuff |
| CN109874946A (en) * | 2019-04-28 | 2019-06-14 | 湖南普菲克生物科技有限公司 | The preparation method and yeast culture and its application of the yeast culture of yolk color can be improved |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1450168A (en) * | 2003-05-09 | 2003-10-22 | 江南大学 | Process for raising glutathion yield by fermentation of tornla yeast |
| CN102232466A (en) * | 2010-05-04 | 2011-11-09 | 洋浦慷民高科生物有限公司 | Bio-protein feed prepared by adopting cellulose raw material and preparation method thereof |
| CN102860408A (en) * | 2012-09-24 | 2013-01-09 | 江西昌丰由由生物科技有限公司 | Preparation method of multifunctional live microorganism preparation for forage |
| CN104855678A (en) * | 2015-06-05 | 2015-08-26 | 沈玉平 | Method for producing tricin by utilizing viable bacteria glycolysis |
| CN105410337A (en) * | 2015-12-11 | 2016-03-23 | 上海邦成生物科技有限公司 | Biological feed additive rich in bioactive peptides and probiotics as well as preparation method of additive |
-
2016
- 2016-11-30 CN CN201611078965.8A patent/CN106551168A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1450168A (en) * | 2003-05-09 | 2003-10-22 | 江南大学 | Process for raising glutathion yield by fermentation of tornla yeast |
| CN102232466A (en) * | 2010-05-04 | 2011-11-09 | 洋浦慷民高科生物有限公司 | Bio-protein feed prepared by adopting cellulose raw material and preparation method thereof |
| CN102860408A (en) * | 2012-09-24 | 2013-01-09 | 江西昌丰由由生物科技有限公司 | Preparation method of multifunctional live microorganism preparation for forage |
| CN104855678A (en) * | 2015-06-05 | 2015-08-26 | 沈玉平 | Method for producing tricin by utilizing viable bacteria glycolysis |
| CN105410337A (en) * | 2015-12-11 | 2016-03-23 | 上海邦成生物科技有限公司 | Biological feed additive rich in bioactive peptides and probiotics as well as preparation method of additive |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109793097A (en) * | 2018-12-26 | 2019-05-24 | 河南巨龙生物工程股份有限公司 | A kind of fermentation process using sugaring tailing and tryptophan mother liquor production biological feedstuff |
| CN109874946A (en) * | 2019-04-28 | 2019-06-14 | 湖南普菲克生物科技有限公司 | The preparation method and yeast culture and its application of the yeast culture of yolk color can be improved |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102168045B (en) | Bacillus subtilis preparation and preparation method thereof | |
| CN101558823B (en) | Fermented liquid feed for Pig and poultry as well as preparation method and application thereof | |
| CN106721261A (en) | One kind is used for swine rearing mixed fermentation fiber feedstuff and preparation method thereof | |
| CN102726596B (en) | Feather protein peptide feed additive and application thereof | |
| CN102907563B (en) | Method for preparing high-activity probiotic preparation for livestock breeding | |
| CN1813562A (en) | Compound microbial fermented fodder nutrient additive and its preparing method | |
| CN101019609A (en) | Solid fermentation process for producing natto bacillus feed additive with bean dregs | |
| CN105995071A (en) | Pig feed for early stage of fattening and preparation method of pig feed | |
| CN106721021A (en) | A kind of piglet antibiotic-free feed and preparation method thereof | |
| CN106591182A (en) | Microecological preparation with bacillus subtilis fermenting astragalus membranaceus and application thereof | |
| CN103734549B (en) | Large-grain milk replacer and preparing method thereof | |
| CN106615933A (en) | Premix mate for laying hens in egg producing period and preparation and application of premix mate | |
| CN101120719B (en) | Microorganism ferment purple sweet potato and preparation method thereof | |
| CN103907749A (en) | Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof | |
| CN101209088A (en) | Microorganism polyzyme additive agent for improving pigling growth and development | |
| CN106721026A (en) | A kind of nonreactive laying cycle of laying hens compound premixed feed and preparation method and application | |
| CN106260504A (en) | A kind of method utilizing beer yeast slurry to produce fermentable moist forage | |
| CN112175846B (en) | Candida utilis strain UCY-11 and application thereof in preparation of fermented hybrid broussonetia papyrifera feed | |
| CN109329567A (en) | It is a kind of using cassava and cassava cauline leaf as the fermented feed of raw material and preparation method | |
| CN103027191A (en) | Phagostimulant fermented feed using bean pulp and bran as protein raw materials and preparation method thereof | |
| CN111685235B (en) | Preparation method of lactobacillus plantarum fermented liquid complete feed for piglets | |
| CN103027184A (en) | Phagostimulant fermented feed using bean pulp, corn protein powder and bran as protein raw materials and a preparation method thereof | |
| CN109315605A (en) | A kind of fermentation process of peanut meal, the fermented peanut meal of this method preparation and its application | |
| CN108835444A (en) | Kind goose feed, preparation method, application and fowl food | |
| CN108902533A (en) | A kind of laying breed feed and its processing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170405 |