CN106591182A - Microecological preparation with bacillus subtilis fermenting astragalus membranaceus and application thereof - Google Patents
Microecological preparation with bacillus subtilis fermenting astragalus membranaceus and application thereof Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention provides a microecological preparation with bacillus subtilis fermenting astragalus membranaceus and an application thereof, wherein the microecological preparation is prepared through fermentation by means of a bacillus subtilis strain FM537 on a fermentation substrate containing astragalus membranaceus water extract liquid. The fermentation substrate comprises 15 g/L of tryptone, 15 g/L of glucose, 50 g/L of beef extract, 0.5 g/L of ammonium sulfate, 1 g/L of sodium chloride and 0.5 g/L of sodium dihydrogen phosphate. The fermentation substrate is prepared with distilled water, wherein pH is regulated to 7.2, and then according to volume ratio, 45-50% of the astragalus membranaceus water extract liquid is added, and finally high-pressure sterilization is carried out to produce the fermentation substrate. Through ultrasonic wave for assisting water extraction of the astragalus membranaceus, the extraction time is greatly reduced. Through selection of suitable fermentation temperature and fermentation time, synergistic effect of the astragalus membranaceus and the bacillus subtilis is improved. The microecological preparation is used as a livestock and poultry feed additive, and can improve animal production performance, digestion rate and body immunity.
Description
Technical field
The present invention relates to a kind of microbial ecological agent of fermentation of bacillus subtilis Radix Astragali and its application, belong in biofermentation
Medicine technical field.
Background technology
In recent years, antibiotic and chemical antibacterials were extensive using brought negative effect in livestock and poultry breeding industry
It is increasingly subject to the attention of people.Such as antibiotic causes substantial amounts of Resistant strain to produce, and causes animal body normal flora to lack of proper care；Together
When, the toxic and side effects that drug residue etc. brings, positive serious harm the health of animal and the mankind, and reduce animal products and exist
Competitiveness on international market, therefore, many countries limit or prohibit application of some antibiotic in animal productiong in succession.
European Union in 2006 promulgate that the decree for disabling antibiotic in feedstuff comprehensively, Japan and Korea were also declared at 2008 and 2011 respectively
Cloth forbids the antibiotic used in animal and fowl fodder.In view of a variety of adverse effects that antibiotic brings, scientific research institution both domestic and external
Research worker is devoted to exploitation always, and some reach maintenance animal by improving animal body immune function, adjusting gastrointestinal tract balance
The novel fodder additive of body health and growth promotion purpose.It is nontoxic (or low that Chinese herbal medicine and animal microecological formulation have
Poison), have no side effect, noresidue, growth promotion and increasing receive the extensive concern of domestic and international researcher the features such as exempt from, application
Species is more and more, scope also more and more extensively, and its good effect effect by substantial amounts of animal productiong verification experimental verification
Really.
Bacillus subtilises as microbial ecological agent in a kind of wide variety of nutritional feed additive, with a lot
Excellent characteristic and effect ensure its extensive application, key factor for it is following some：
(1) growth and breeding condition is not strict.A. nutritional requirement is relatively low, containing wheat bran, Semen Maydiss, the livestock and poultry diet of bean cake
Well can grow in culture medium；B. growth temperature is more wide in range, optimum growth temperature at 37 DEG C or so, but at 30～40 DEG C
In the range of can also breed well；C., in the range of p H-number 6～8, living bacteria count to be reached, acid-base value are not to which
Critical influence factor is bred quickly.
(2) strong stress resistance.Bacillus subtilises most outstanding feature is exactly to form one kind in its later stage for growing to have
The spore of multi-layer film structure, is characterized in that water content is low, wall thickness and it is fine and close, 2-6 dipicolinic acid content is high, containing thermostable enzyme, this
A little features cause its resistance to heat, acid, alkali, ultraviolet and chemicalses high, and the hay applied in usual Production of Livestock and Poultry
Effective ingredient in bacillus cereuss product is present with the form of spore.Such as in 80 DEG C of hot water heat treated 10 minutes its
Survival rate is more than 80%；6 hours survival rates are processed under artificial gastric acid environment of the p H-number equal to 2 still above 76%；In quality
Concentration be 0.3% Fel Sus domestica saline solution in process 4 hours after survival rate more than 90%.In this, resistance is which in animal husbandry
Extensively application is had laid a good foundation.
(3) fully natural green is efficient.Bacillus subtilises have safe no drug residue as the feed additive that China ratifies,
The characteristics of having no toxic side effect, in addition its high efficiency show as spore into after intestinal, can bring back to life rapidly in intestinal epimere, resurrection rate
Nearly 100%.
(4) improve efficiency of feed utilization, improve livestock and poultry production performance.Bacillus subtilises enter digestion with the form of spore
Road, brings back to life rapidly and quickly breeds in intestinal epimere, can produce a large amount of materials for participating in body substance metabolism in the process
Such as digestive enzyme (protease, amylase, lipase, saccharifying enzyme), aminoacid and various vitamin B group (thiamine, riboflavin, cigarette
Acid etc.), absorption of the body to protein, trace element (calcium, ferrum) and vitamin D can be promoted, improved body and digested and assimilated work(
Can, efficiency of feed utilization is improved, the generation of punishment in advance phenomenon is prevented, promotes growth of animal.
(5) improve the premunition of animal body.Bacillus subtilises are in production and the meeting during breeding in animal and bird intestines
The volatile fatty acids such as acetic acid, propanoic acid and butanoic acid are produced, these acidic materials can reduce intestinal p H, so as to suppress pathogen
Such as colibacillary growth, this acts on and nascent young stock is especially apparent.Other metabolites for example subtilin, polymyxin,
Gramicidin etc. can also suppress the breeding of enteropahtogenic microganism.
(6) strengthen animal body immunity function.Compound micro-ecological preparation containing bacillus subtilises can be with stimulating animal intestinal
The development of road immune organ, strengthens the phagocytic function of macrophage and improves vaccine immunity antibody horizontal, enhancing human body immunity machine
Can, it is good immunostimulant.
The Radix Astragali can be with the specific immunity (humoral immunization and cellular immunization) of enhancing body and non-specific immunity.It is yellow
Stilbene is obviously promoted the research such as effect, Xiang Jie and shows to the antibody tormation function of normal body, gives infection listerial mice
Injection astragalus polysaccharidess, in its serum, IgG titres are significantly improved, and illustrate that astragalus polysaccharidess can promote the activation of B cell and propagation, are increased
The humoral immunization and cellular immunization of strong host resists the infection of pathogen protecting host.Radix Astragali water extract can significantly improve liver
Total complement (the CH of scorching patient50) and point complement (C3) content, the T cell breeder reaction that concanavalin A is excited can be made substantially to increase,
There is potentiation to the function of T lymphocytes simultaneously, additionally it is possible to improve the killing activity and pulmonary macrophage of NK cells
Ability.In addition, the polysaccharide component in the Radix Astragali can be with the generation of inducing interferon and participation immunomodulating.
The Radix Astragali can improve the ability of body anti-stress.Radix Astragali extract can make adrenocortical hormone and promote adrenal gland's skin
Matter hormone exhausts that the swimming time of mice is obviously prolonged, and strengthens mice to the tolerance of various anoxia model and makes adrenal gland's weight
Amount is dramatically increased.The low temperature resistant ability of mice can also be strengthened, extend the life span of normal and weak mice.Zhou Xianqing etc. is studied
Show, the Radix Astragali have it is antiacid stress effect, in feedstuff add the Radix Astragali not only to Acid stress caused by endocrine dysfunction
With adjustment effect, and there is protective effect to the immunologic hypofunction caused by Acid stress.
The focus of novel fodder additive research at present mainly has the following aspects：Improve animal body immunity；Increase
The function of strong body digestive system, improves food conversion ratio；Low-residual, green, efficiently.As the Radix Astragali itself is containing abundant battalion
Foster material (polysaccharide, saponin, flavone and abundant trace element etc.), can improve the ability of animal body immunity and anti-stress again, and
Probiotic bacteria can improve the function of animal body digestive system, and the two combination can be played preferably effect.On the one hand it is yellow
, to the selective stimulation of intestinal flora activity, another aspect intestinal is to medicine in body for polysaccharide component in stilbene
Interior metabolism and biotransformation are played an important role.
It is more to the research of the lactic acid bacteria fermentation Radix Astragali (solid and liquid fermentation), astragalus root dregs and Radix Astragali extract at present,
And it is fewer with regard to the relevant report of Radix Astragali fermentation culture bacillus subtilises.Research contents is focused primarily upon and is improved by fermenting
The change of Radix Astragali drug effect after the change of active constituent content after extraction to Radix Astragali effective ingredient, fermentation, fermentation, the Radix Astragali (and its
Effective ingredient) application effect of the probiotics composite preparation in animal productiong etc..
The content of the invention
In order to overcome the defect of prior art, present invention aim at providing a kind of astragalus polysaccharidess and bacillus subtilises increasing
The microbial ecological agent of the high fermentation of bacillus subtilis Radix Astragali of rate and its application.
For achieving the above object, the present invention is adopted the following technical scheme that：
A kind of microbial ecological agent of the fermentation of bacillus subtilis Radix Astragali, the microbial ecological agent are by one plant of bacillus subtilis
Bacterium FM537 is prepared from the fermentation substrate top fermentation containing Radix Astragali aqueous extract, wherein, the fermentation substrate is：Trypsin
Peptone 15g/L, glucose 15g/L, Carnis Bovis seu Bubali cream 50g/L, ammonium sulfate 0.5g/L, Sodium Chloride 1g/L, sodium dihydrogen phosphate 0.5g/L, distillation
Water is prepared, and adjusts pH to be 7.2, then according to volume ratio adds the Radix Astragali aqueous extract of 45-50%, autoclaving is obtained final product.
Further, the fermentation is preferably 37 DEG C of -39 DEG C of fermentation culture 48-72 hours.
Further, the Radix Astragali aqueous extract is prepared by ultrasonic assistant extraction method, specially：Take the Radix Astragali add water it is pan-fried
Boil once, mixture will be decocted and poured in container, then container is moved in ultrasonator, 0.5- is vibrated under 25-30KHz
1h, is filtered, and takes supernatant, obtains final product Radix Astragali aqueous extract.
Further, the concentration of the Radix Astragali aqueous extract is：Extracted by above-mentioned ultrasonic assistant per 30 grams of Radixs Astragali
Method correspondence obtains the Radix Astragali aqueous extract of 200～300mL.
Further, the inoculum concentration of the bacillus subtilises is calculated as with viable count：107-108CFU/mL fermentation substrates.
Bacillus subtilises FM537 of the present invention is stored in Chinese microorganism strain guarantor on the 15th in August in 2016
Hide administration committee's common micro-organisms center (abbreviation CGMCC, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese section
Institute of microbiology of institute, postcode 100101), deposit number：CGMCC No.12848, Classification And Nomenclature：Bacillus subtilises
(Bacillus subtilis)。
Bacillus subtilises FM537 of the present invention is that a plant for obtaining is screened from the soil sample of Turpan, Xinjiang flame hill
Produce thermoase and high-temperature amylase strain.The spore size of the bacterium is about 1.2 μm of 0.7 μ m, rounded, in improvement meat soup
It is that rough surface is opaque that bacterium colony is cultivated on solid medium, and culture on liquid culture occurs buckling phenomena.This plant of spore bar
Bacterium can produce thermostable proteinase, amylase, cellulase, mannase and some peptides etc..By what is produced to the bacterial strain
Protease and amylase carry out the research of enzymatic property and find that the optimum temperature of its produced protease is 58 DEG C, at 80 DEG C
Reason 3min, remains to retain more than 80% enzyme activity, and 90 DEG C of process 3min can equally retain more than 80% enzyme activity；Produced starch
The optimum temperature of enzyme is 60 DEG C, and 80 DEG C of process 3min remain to retain more than 80% enzyme activity, and 90 DEG C process 3min, equally
More than 80% enzyme activity can be retained, cellulase and mannase have higher temperature tolerance, the cellulase of generation
700U/g and 400U/g can be reached with the enzyme activity value of mannase.This plant of bacterium can not only produce various lists of strong stress resistance
Enzyme, and the content of the mannase and cellulose of generation is very high, by this bacillus cereus and Radix Astragali water co-fermentation culture
The polyoses content in Radix Astragali water can be greatly improved.
The present invention also provides the microbial ecological agent of the above-mentioned fermentation of bacillus subtilis Radix Astragali as additive for farm animal feed
Purposes, especially as the purposes of livestock and fowl drinking water additive.Microbial ecological agent of the present invention is added in poultry drinking water
Weight/mass percentage composition is 0.5%-1%.
Beneficial effect：
The microbial ecological agent of the fermentation of bacillus subtilis Radix Astragali of the present invention, connects in the fermentation substrate containing Radix Astragali aqueous extract
Plant fermentation of bacillus subtilis to form.By the access bacillus subtilises of suitable dose, bacillus subtilises and Radix Astragali water are made
Synergistic function is produced between extracting solution, traditional direct additive that bacillus subtilises and the Radix Astragali are mixed is compared, is carried
The high content of astragalus polysaccharidess isoreactivity material and bacillus subtilises, reduces the toxic and side effects of the Radix Astragali, low production cost.
Further, in fermentation substrate, the volume fraction of Radix Astragali aqueous extract is 45-50%, plays hay to greatest extent
The bioconversion function of bacillus cereuss, Bacillus subtilis number increase an order of magnitude, and the gaining rate of astragalus polysaccharidess content reaches
19.50%, the gaining rate of Astragaloside content reaches 26.23%, and the content gaining rate of total flavones reaches 11.61%.
The preparation method of the microbial ecological agent of the fermentation of bacillus subtilis Radix Astragali of the present invention, by selecting suitable fermentation temperature
Degree and fermentation time, improve crude polysaccharides, astragaloside, flavone and bacillus subtilis bacterial content in the microbial ecological agent for preparing, carry
The high Radix Astragali and the synergistic action effect of bacillus subtilises, it is easy to operate, it is easy to control, it is suitable to industrial application.
The microbial ecological agent of the fermentation of bacillus subtilis Radix Astragali of the present invention as additive for farm animal feed, using hay spore
Bacillus fermentation Radix Astragali aqueous extract, makes effective ingredient and producing bacillus subtilis life synergistic function in Radix Astragali aqueous extract,
Increase bacillus subtilises viable bacteria amount and astragalus polysaccharidess, astragaloside and flavone equal size, raise the microbial ecological agent addition
In material, breeding performonce fo animals, digestibility and immunity of organisms are improved.
Specific embodiment
Below by the specific embodiment narration present invention.Unless stated otherwise, technological means used in the present invention
It is method known in those skilled in the art.In addition, embodiment be interpreted as it is illustrative, and it is unrestricted the present invention
Scope, the spirit and scope of the invention are limited only by the claims that follow.
The percentage sign " % " being related in embodiment, if not specified, refers to mass percent, and the percentage ratio of solution refers to
Grams containing solute in 100ml, the percentage ratio between liquid refer to the volume ratio of the solution at 25 DEG C.
Embodiment 1：
A kind of microbial ecological agent of the fermentation of bacillus subtilis Radix Astragali, the microbial ecological agent are by one plant of bacillus subtilis
Bacterium FM537 is prepared from the fermentation substrate top fermentation containing Radix Astragali aqueous extract, wherein, the fermentation substrate is：Trypsin
Peptone 15g/L, glucose 15g/L, Carnis Bovis seu Bubali cream 50g/L, ammonium sulfate 0.5g/L, Sodium Chloride 1g/L, sodium dihydrogen phosphate 0.5g/L, distillation
Water is prepared, and adjusts pH to be 7.2, then according to volume ratio adds the Radix Astragali aqueous extract of 45-50%, autoclaving is obtained final product；It is described
The deposit number of bacillus subtilises FM537 is CGMCC No.12848.
The bacillus subtilises are inoculated into fermentation substrate with secondary seed solution form, and its preparation method is：By hay bud
Spore bacillus FM537 lines LB agar plates, and 37 DEG C of incubated overnight, picking single bacterium colony are inoculated in 10mL LB fluid mediums,
Primary seed solution is inoculated in improvement nutrient broth training by 1% inoculum concentration as primary seed solution by 150rpm, 37 DEG C of incubated overnight
Foster base, 150rpm, 37 DEG C of incubated overnight obtain secondary seed solution.
It is described to improve consisting of for nutrient broth medium：Tryptone 15g/L, glucose 15g/L, Carnis Bovis seu Bubali cream 50g/L,
Ammonium sulfate 0.5g/L, Sodium Chloride 1g/L, sodium dihydrogen phosphate 0.5g/L, distilled water are prepared, and adjust pH to obtain final product for 7.2.
The inoculum concentration of the bacillus subtilises is calculated as with viable count：107-108CFU/mL fermentation substrates.
The fermentation is preferably 37 DEG C of -39 DEG C of fermentation culture 48-72 hours.
The Radix Astragali aqueous extract is prepared by ultrasonic assistant extraction method, specially：Take the Radix Astragali to add water to cook once, will
Decoct mixture to pour in container, then container is moved in ultrasonator, 0.5-1h is vibrated under 25-30KHz, carried out
Filter, takes supernatant, obtains final product Radix Astragali aqueous extract.Wherein, the concentration of the Radix Astragali aqueous extract is：Per 30 grams of Radixs Astragali by above-mentioned
Ultrasonic assistant extraction method correspondence obtains the Radix Astragali aqueous extract of 200～300mL.
The microbial ecological agent of the fermentation of bacillus subtilis Radix Astragali described in the present embodiment can be used as the use of additive for farm animal feed
On the way, the especially purposes of livestock and fowl drinking water additive；The microbial ecological agent of the fermentation of bacillus subtilis Radix Astragali is added to poultry
Weight/mass percentage composition in drinking water is 0.5%～1%.
By several contrast project study Radix Astragali water extract with the present embodiment technical scheme and bacillus subtilises
FM537 after fermentation be mutually improved effect, as a result as shown in Table 1 and Table 2.
Table 1：The changes of contents of astragalus polysaccharidess, astragaloside and flavone before and after fermentation
Table 2：Impact of the Radix Astragali aqueous extract to bacillus subtilises number of viable
As can be seen from the above results, in fermentation substrate Jing bacillus subtilises of the present embodiment containing Radix Astragali aqueous extract
After FM527 fermentations, the content of astragalus polysaccharidess, astragaloside and total flavones in fermentation substrate has different degrees of raising.And
And, the fermentation substrate containing Radix Astragali aqueous extract compares the fermentation substrate Jing bacillus subtilises FM537 without Radix Astragali aqueous extract
After fermentation, the quantity of bacillus subtilises has increasing substantially for an order of magnitude.By Radix Astragali aqueous extract and hay spore
Microbial ecological agent prepared by the synergism of bacillus, not only shortens the time of extraction, and improves astragalus polysaccharidess, Radix Astragali first
The quantity of glycosides, the content of flavone and bacillus subtilises, realizes the positive role being mutually improved.
Embodiment 2：
Application effect of the microbial ecological agent of the fermentation of bacillus subtilis Radix Astragali of the present invention in laying hen feeding.
The extra large blue brown laying hen for selecting 1440 300 ages in days, body weight and laying rate close, is randomly divided into 3 process, at each
10 repetitions of reason, each repeats 38 chickens.Treatment group 1 is matched group, without the microbial ecological agent of the present invention；Treatment group 2 and 3
For test group, respectively in its drinking water the embodiment of the present invention 1 of addition 0.5% and 1% microbial ecological agent.Feeding identical
Daily ration, experimental period are 70 days, after off-test, calculate the indexs such as average feed intake, laying rate, feedstuff-egg ratio, egg production, respectively from
Each treatment group picks up 30 pieces of egg, detection eggshell strength, shell thickness, Hough unit, yolk color and egg shape index at random.Basic day
Grain formula is as shown in table 3：
Table 3：Diet Formula and trophic level
Table 4：Impact of the microbial ecological agent of the present invention to performance in layers
* difference of going together letter representation significant differences
Table 5：Impact of the microbial ecological agent of the present invention to laying hen Egg Quality
* same column difference letter represents significant difference.
As a result as shown in table 4 and table 5, compared with matched group, respectively in drinking water addition 0.5% and 1% the present invention
Microbial ecological agent is conducive to the raising of Layer Production Performance and Egg Quality, significantly improves laying rate, egg production, reduces material
Egg ratio, significantly improves eggshell strength, thickness and yolk color.
Embodiment 3：
Impact of the microbial ecological agent of the fermentation of bacillus subtilis Radix Astragali of the present invention to meat chicken production performance.
216 1 age in days healthy AA broiler chicken are chosen, 3 process are randomly divided into, each processes 6 repetitions, each repeats 12
Chicken.Treatment group 1 is matched group, is not added with the microbial ecological agent of the present invention；Treatment group 2 and 3 is test group, respectively in drinking water
1 microbial ecological agent of the embodiment of the present invention of middle addition 0.5% and 1%.Feeding identical daily ration, 20 days experimental periods, experiment terminates,
Calculate average daily gain, average daily gain, feedstuff-meat ratio and death rate.Off-test morning to broiler venous blood collection,
Be stored in after preparing serum in -20 DEG C of refrigerators, IgG and IgM in each test group serum is detected respectively using test kit and microplate reader,
Daily ration is constituted and nutritional labeling is shown in Table 6.
Table 6：Diet Formula and trophic level
Table 7：Impact of the microbial ecological agent of the present invention to meat chicken production performance
* difference of going together letter representation significant differences
As a result as shown in table 7, add microbial ecological agent of the present invention (processing 2 and 3) in drinking water, it is aobvious compared with matched group
What is write improves broiler daily gain, reduces feedstuff-meat ratio and mortality rate, while from from immune indexes, the concentration of IgG and IgM is bright
It is aobvious to increase, while enhancing immunologic function.But no notable difference between processing 2 and processing 3 groups.
Embodiment 4：
Impact of the microbial ecological agent of the fermentation of bacillus subtilis Radix Astragali of the present invention to pig production performance.
From the nursery pig 81 of 42 ages in days or so, point 3 process, each processes 3 repetitions, and each repeats 9 pigs.Place
Reason group 1 be matched group, the microbial ecological agent being not added with the present invention；Treatment group 2 and 3 is test group, is added respectively in drinking water
Plus 0.5% and 1% 1 microbial ecological agent of the embodiment of the present invention.Feeding identical daily ration, 25 days experimental periods, experiment terminates, and calculates
Average daily gain, average daily gain, feed-weight ratio and statistics diarrhoea situation.Daily ration is constituted and nutritional labeling is shown in Table 8.
Table 8：Daily ration is constituted and nutritional labeling is shown in Table
Table 9：Impact of the microbial ecological agent of the present invention to weaned piglets
* difference of going together letter representation significant differences.
As a result as shown in table 9, add microbial ecological agent (treatment group 2 and 3) of the present invention, it is significant compared with matched group to improve
Piglet daily gain, daily ingestion amount, reduce feed-weight ratio, and diarrhoea head is also significantly reduced.But do not have between treatment group 2 and 3
Notable difference.
Embodiment 5：
Using the detection case of bacterium in feces after fermentation of bacillus subtilis microbial ecological agent of the present invention.
120 1 age in days healthy AA broiler chicken are chosen, 2 process are randomly divided into, each processes 6 repetitions, each repeats 10
Chicken.Treatment group 1 is matched group, is not added with the microbial ecological agent of the present invention；Treatment group 2 adds this by 0.5% in its drinking-water
The microbial ecological agent of inventive embodiments 1, feeds identical daily ration, after 15 days, aseptic to take feces, to the escherichia coli in feces,
Lactic acid bacteria and bacillus cereuss are detected.
Table 10：After addition microbial ecological agent of the present invention in feces bacterium testing result
As a result as shown in table 10, add the treatment group 2 of microbial ecological agent of the present invention, substantially ratio is not added with escherichia coli quantity
Matched group reduce, lactic acid bacteria and Bacillus subtilis number substantially increase.There is suppression by adding microbial ecological agent of the present invention
The effect of harmful bacteria and increase probioticss quantity.
Claims (7)
1. a kind of microbial ecological agent of the fermentation of bacillus subtilis Radix Astragali, it is characterised in that the microbial ecological agent is by a plant
Bacillus subtilises FM537 is prepared from the fermentation substrate top fermentation containing Radix Astragali aqueous extract, wherein, the fermentation substrate
For：Tryptone 15g/L, glucose 15g/L, Carnis Bovis seu Bubali cream 50g/L, ammonium sulfate 0.5g/L, Sodium Chloride 1g/L, sodium dihydrogen phosphate
0.5g/L, distilled water are prepared, and adjust pH to be 7.2, then according to volume ratio adds the Radix Astragali aqueous extract of 45-50%, high pressure goes out
Bacterium obtains final product；The deposit number of the bacillus subtilises FM537 is CGMCC No.12848.
2. a kind of microbial ecological agent of the fermentation of bacillus subtilis Radix Astragali as claimed in claim 1, it is characterised in that described
Ferment is 37 DEG C of -39 DEG C of fermentation culture 48-72 hours.
3. a kind of microbial ecological agent of the fermentation of bacillus subtilis Radix Astragali as claimed in claim 1, it is characterised in that described withered
The inoculum concentration of careless bacillus cereuss is calculated as with viable count：107-108CFU/mL fermentation substrates.
4. a kind of microbial ecological agent of the fermentation of bacillus subtilis Radix Astragali as claimed in claim 1, it is characterised in that the Huang
Stilbene aqueous extract is prepared by ultrasonic assistant extraction method, specially：Take the Radix Astragali to add water to cook once, mixture will be decocted and poured into
In container, then container is moved in ultrasonator, 0.5-1h is vibrated under 25-30KHz, filtered, take supernatant, obtained final product
Radix Astragali aqueous extract.
5. a kind of microbial ecological agent of the fermentation of bacillus subtilis Radix Astragali as claimed in claim 4, it is characterised in that the Huang
The concentration of stilbene aqueous extract is：The Radix Astragali of 200～300mL is obtained per 30 grams of Radixs Astragali by above-mentioned ultrasonic assistant extraction method correspondence
Aqueous extract.
6. a kind of microbial ecological agent of the arbitrary described fermentation of bacillus subtilis Radix Astragali of claim 1-5 adds as animal and fowl fodder
Plus the purposes of agent.
7. purposes as claimed in claim 6, it is characterised in that the microbial ecological agent is added to the quality in poultry drinking water
Percentage composition is 0.5%-1%.
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| CN111000892A (en) * | 2020-01-10 | 2020-04-14 | 河南天昊鸿发生物技术有限公司 | Compound additive for improving laying rate of poultry and preparation method and application thereof |
| CN111228460A (en) * | 2020-01-10 | 2020-06-05 | 河南天昊鸿发生物技术有限公司 | Microecological preparation for improving immunity of livestock and poultry and preparation method and application thereof |
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| CN111543549A (en) * | 2020-06-02 | 2020-08-18 | 烟台绿叶动物保健品有限公司 | Preparation method and application of traditional Chinese medicine microecological preparation |
| CN112471351A (en) * | 2020-11-14 | 2021-03-12 | 南京雷德蒙德农业科技有限公司 | Composition for preventing livestock and poultry diarrhea and preparation method thereof |
| CN112704150A (en) * | 2020-12-29 | 2021-04-27 | 河南农业大学 | Fermented traditional Chinese medicine immunopotentiator and preparation method and application thereof |
| CN112831445A (en) * | 2021-03-09 | 2021-05-25 | 湖北大学 | Bacillus subtilis S4 derived from intestinal tracts of insect soldier fly larvae and application thereof in aspect of efficiently degrading proteins at low temperature |
| CN112831445B (en) * | 2021-03-09 | 2022-07-05 | 湖北大学 | Bacillus subtilis S4 derived from intestinal tracts of insect soldier fly larvae and application thereof in aspect of efficiently degrading proteins at low temperature |
| CN113773985A (en) * | 2021-08-20 | 2021-12-10 | 江南大学 | Bacillus subtilis for improving traditional Chinese medicine polysaccharide yield and regulating skin barrier and immunity |
| CN113773985B (en) * | 2021-08-20 | 2023-08-25 | 江南大学 | Bacillus subtilis for improving yield of traditional Chinese medicine polysaccharide and regulating skin barrier and immunity |
| CN115119899A (en) * | 2022-08-30 | 2022-09-30 | 山东泰山生力源集团股份有限公司 | Mixed-strain fermentation microbial preparation and preparation method thereof |
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