CN102356816B - Animal health care probiotics leavening capable of improving meat quality and application thereof - Google Patents

Animal health care probiotics leavening capable of improving meat quality and application thereof Download PDF

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Publication number
CN102356816B
CN102356816B CN2011102790858A CN201110279085A CN102356816B CN 102356816 B CN102356816 B CN 102356816B CN 2011102790858 A CN2011102790858 A CN 2011102790858A CN 201110279085 A CN201110279085 A CN 201110279085A CN 102356816 B CN102356816 B CN 102356816B
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component
probiotics
starter
animal
leavening
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CN2011102790858A
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Chinese (zh)
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CN102356816A (en
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杨博
唐家毅
王永华
李东华
曹昱
谢应根
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华南理工大学
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Abstract

The invention discloses an animal health care probiotics leavening capable of improving meat quality and application thereof. The leavening includes a component A, a component B and a component C, and a live bacteria proportion in the component A, component B and component C is 1: (0.2-5) : (0.1-5); the component A is a composition of one or two from plant lactobacillus and manure enterococcus faecalis; the component B is composition of one or two from lactobacillus acidophilus and bifidobacterium; and the component C is saccharomyces cerevisiae. The leavening of the invention can be utilized to prepare probiotics bacteria liquid and fermented feed for animal culture in order to increase meat quality of culture animals. The technical method of the invention is capable of improving meat quality, simple and practical.

Description

A kind of animal health probiotics leaven and application thereof that improves meat
Technical field
The present invention relates to a kind of animal health probiotics leaven and utilize this starter to produce the method for probiotics bacterial liquid and fermented feed.
Background technology
Probiotics preparation be a class under the microecology theoretical direction, contain viable bacteria or comprise the active bacteria formulation of bacterium thalline and meta-bolites thereof through what special process was made.Its viable bacteria can field planting in animal intestinal, adjusts and keep normal microecological balance in enteron aisle, and stimulates specificity and the non-specific immunity of body, has to promote nutrient digestion to absorb and the effect of animal growth and development.Probiotic bacterium has broad application prospects in feed and herding production as a kind of green, safe and effective additive.After probiotic bacterium enters animal intestinal, with microbial interaction wherein, produce symbiosis, dwell and the very complicated relation such as give birth to, compete or engulf.Think at present its mechanism of action mainly contain following some: (1) increases intestinal beneficial bacterium quantity, suppresses the harmful bacteria growth, recovers or keep the microecological balance of enteron aisle, thereby suppresses the Growth and reproduction of pathogenic bacteria; (2) beneficial microorganism has Ant agonism to pathogenic micro-organism in vivo, can compete the inhibition pathogenic micro-organism adheres on intestinal epithelial cell, fight for limited nutritive substance and ecological site with pathogenic micro-organism, thereby be unfavorable for the growth and breeding of pathogenic micro-organism; (3) fermentable carbohydrate produces the voltaile fatty acids such as lactic acid and acetic acid, propionic acid, butyric acid, reduces pH value in enteron aisle, thereby suppresses the growth of pathogenic bacterium in enteron aisle; (4) can produce various nutritive substances such as VITAMIN, amino acid, unknown somatomedin etc., participate in body metabolism, improve food conversion ratio, promote growth of animal; (5) stimulate and activate the immunity system of body, the Promote immunity function is ripe, improves the immune defense function of body.The immunity system of animal body directly affects the production performance of animal.Probiotic bacterium can be improved host's immune defence mechanism, except safeguarding this nonimmune regulating effect of intestinal microecology balance, can also be by stimulating and activate specificity and the non-specific immunity of body, the Promote immunity function is ripe, improves the defensive enginery of body.
Substitute antibiotics is one of major function of probiotic bacterium, becomes the focus of domestic and international research for the application of probiotic bacterium raising animal immune.Studies show that the immuno-potentiation of probiotic bacterium to animal is mainly that its thalline or meta-bolites are to the effect of Mucosal Immunity.After probiotic bacterium enters body, adjusting intestinal microflora makes the intestinal microecology system be in best equilibrium state, its cell wall constituent, meta-bolites, somatic cells etc. are the Intestinal Mucosal Immunity system of possibility stimulating animal all, associated lymphoid tissue in the activation intestinal mucosa strengthens the SIgA antibody-secreting, improve immune insight, and induce T, bone-marrow-derived lymphocyte and scavenger cell to produce cytokine, activate systemic immune system by lymphocyte recirculation, thus the non-specific and specific immune function of enhancing body.Probiotic bacterium to the approach of immunity system hormesis may be the meta-bolites of microorganism directly by common epithelial cell or through the tight connection gap between epithelial cell, might be also that microorganism cells itself is sent to the macrophage that is arranged in M cell packing etc. by pinosome by microfold cell (M cell).Antigenic substance is through the M cell, be by antigen presenting cell such as scavenger cell and dendritic cell processing and pass T cell and B cell, produce the antigen-specific b lymphoblast, after germinal center's breeding, move to mucous membrane and gland tissue at a distance by blood flow, and further differentiation and maturation is plasmocyte, secretion IgA, IgA optionally with the polymeric IgA receptors bind of epithelial cell, then strides across epithelial cell and is discharged in mucous membrane or glandular secretion thing after forming binary or many bodies.Takahas etc. confirm in experiment, and the probiotics bacterial somatocyte in enteron aisle, meta-bolites etc. can enter intestines endolymph tissue through the M cell, and body's immunity is wielded influence.Genus bacillus can be activated gut-associated lymphoid tissue, speed the growth of immune organ, and the quantity of T, bone-marrow-derived lymphocyte increases, and the body fluid of animal and cellular immune level are improved, and the enhancing body resistance against diseases prevents the generation of disease.The yeast cell wall contains zymosan, and its main active ingredient is grape Ruan, mannosans, chitin, can improve the immunizing power of animal.
There is the situation of microbiotic, additive abuse in the suitable vast scale of tradition cultivation, uses microbiotic, although can prevent the generation of some diseases, also with animal as for sub-health state, the growth of animal state also has influence on mouthfeel and the quality of meat.In the application of probiotic bacterium, people also find, by the use of probiotic bacterium, stopped antibiotic use, made animal be in a kind of more natural growth conditions, animal body is healthy, virus disease significantly reduces, and the quality of meat also increases, but lacks systematic research.
People are used for the probiotic bacterium means and carry out the health cultivation the growing interest of food-safety problem and the raising day by day of food quality requirement, improve meat food safety and lifting meat quality, meet the needs of social development.At present, people solve antibiotic abuse problem to have carried out more research to using probiotic bacterium, and the application scheme of comparative maturity has been arranged, but for how improving meat, correlative study also is close to and is in blank.
Summary of the invention
For solving the deficiencies in the prior art, the present invention aims to provide a kind of animal health probiotics leaven that improves meat, utilizes this starter production probiotic products, and resulting probiotic bacterium is applied to cultivation, when realizing animal health, realize the obvious lifting of meat.
Milk-acid bacteria is important functional microbial inoculum, and in the subordinate classification, the lactic acid bacteria class that is usually used in animal cultivation mainly contains 3 classes: 1) lactobacillus: the Bulgarian kind of Lactobacillus acidophilus, short lactobacillus, lactobacterium casei, plant lactobacillus and lactobacillus delbruckii etc.; 2) genus bifidobacterium: bifidus longum bb, bifidobacterium adolescentis, bifidumbacterium bifidum and bifidobacterium breve; 3) Coccus: enterococcus faecalis, urine enterococcus, Lactococcus lactis breast subspecies, lactococcus lactis subsp, saliva chain coccus thermophilous subspecies and intermediate chain coccus etc.But, the present invention's discovery, from the effect at animal cultivation, cannot be simply according to this sorting technique.
Purpose of the present invention is achieved through the following technical solutions:
A kind of animal health probiotics leaven that improves meat comprises A, B and C three components, and the viable count ratio in component A, B, C is 1: (0.2~5): (0.1~5); Described component A is one or both the composition in plant lactobacillus and enterococcus faecalis; Described B component is one or both the composition in Lactobacterium acidophilum and bifidus bacillus; Described component C is S. cervisiae.
Preferably, in described component A, B and C, the viable count ratio is 1: (0.5~3): (1~2).
Preferably, the blending ratio of described component A plant lactobacillus and enterococcus faecalis viable count is 1: (1~2); The blending ratio of described B component Lactobacterium acidophilum and the number of active bifid bacteria is 1: (1~2).
Preferably, described bifidus bacillus is one or more the composition in baby's bifid, youth bifid, long bifid, short bifid, not tally bifid and angle bifidus bacillus.
Described starter is used for enlarged culturing and produces probiotics bacterial liquid, then this probiotics bacterial liquid is used for feeding animals.The basic skills principle of utilizing starter to produce probiotics bacterial liquid is that microorganism spreads cultivation, normally starter is joined in suitable substratum, this substratum contains organic carbon source and organic nitrogen source, cultivate certain hour at suitable temperature, as 35 ℃ of sealed fermentings 36 hours, can obtain probiotics bacterial liquid.Preferably, described probiotics bacterial liquid adds in feed in the ratio of massfraction 0.8%~1.2%.
The preparation method of above-mentioned starter can adopt the freezing formulation of deep liquid, also can adopt the bacterium powder of freeze-dried vaccine powder or employing mode drying to mix.The production of probiotic bacterium belongs to routine operation, can produce according to relevant references explanation.
Application of the present invention further comprises and utilizes probiotics bacterial liquid and feed mixed fermentation to produce the use of fermented feed feeding animals.The preparation method of described fermented feed is: probiotics bacterial liquid and normal diet mix according to 1: 10 ratio, add water and are adjusted to fermented feed water content 30%~50%, sealing and fermenting 2 days; Described fermented feed can use separately, also can be with normal diet by the mass ratio of 1: 10 use that is mixed.
Adopt starter of the present invention to prepare probiotics bacterial liquid, be used for comprising the animal cultivation of pig, ox, rabbit, chicken, duck, goose, fish, shrimp, crab etc., found that all meat in various degree improves, illustrate that this starter has suitability widely.
The present invention compared with prior art has following advantage:
1) has the function of improving the animal meat;
2) utilize starter production probiotics bacterial liquid and fermented feed simple and easy to do;
3) has good animal health function.
Embodiment
Introduce in more detail by the following examples enforcement of the present invention.But the present invention is not limited only to this.
Embodiment 1
1. the preparation of probiotics leaven
Adopt the bacterium powder of freeze-drying, survey viable count, according to viable count ratio preparation probiotics leaven, (plant lactobacillus+enterococcus faecalis)=2,000,000,000/g wherein, both ratio is 1: 1; (Lactobacterium acidophilum+bifidobacteria infantis)=3,000,000,000/g, both ratio is 1: 1; Yeast saccharomyces cerevisiae 3,000,000,000/g.
2. the preparation of probiotics bacterial liquid:
Preparation MRS substratum 20L adds the 10g starter, and 35 ℃ of sealed fermentings 36 hours are standby.
3. culture experiment
70 52 of age in days Large Whites, mean body weight 31.2kg is divided into two groups at random, one group of normal diet of feeding, another group is added with 1% (massfraction) probiotics bacterial liquid in normal diet, feed to 160 ages in days, gets at random 3 for every group and entrusts the slaughterhouse to slaughter.
4. evaluation of meat quality method
Get the longissimus dorsi muscle of experimental group and control group pig, 1% salt poach 10 minutes, section is carried out sensory evaluation from tender degree, succulence, local flavor 3 aspects of meat, and evaluation group is by forming more than 8 people, according to double-blind evaluation.Every index full marks are 100 minutes, and the higher representation quality of mark is higher, the contrast of averaging.
5. results and discussions
Table 1
Assessment item Experimental group Control group
Tender degree ??82.6 ??82.3
Succulence ??85.8 ??82.5
Local flavor ??86.6 ??81.2
By table 1 result as seen, the tender degree index of experimental group and control group difference are little, and obviously are better than control group in succulence and local flavor, illustrate, adopt interpolation probiotic bacterium of the present invention to cultivate and can obviously improve meat quality.
Embodiment 2
1. the preparation of probiotics leaven:
Adopt the bacterium powder of freeze-drying, survey viable count, according to viable count ratio preparation probiotics leaven, the starter total count is 10,000,000,000/g, (plant lactobacillus+enterococcus faecalis)=2,000,000,000/g wherein, and both ratio is 1: 2; (Lactobacterium acidophilum+bifidobacteria infantis)=5,000,000,000/g, both ratio is 1: 2; Yeast saccharomyces cerevisiae 3,000,000,000/g.
2. the preparation of probiotics bacterial liquid
Preparation MRS substratum 20L adds the 10g starter, and 35 ℃ of sealed fermentings 36 hours are standby.
3. the production of fermented feed
Probiotics bacterial liquid and complete feed mix according to 1: 10 ratio, add water and are adjusted to fermented feed water content 40% left and right, and be standby with sealing and fermenting in plastics bag 2 days.
4. culture experiment
78 78 of age in days Large Whites are divided into two groups at random, one group of normal diet of feeding, and another group is added with 10% fermented feed in normal diet, feed to 156 ages in days, gets at random 3 for every group and entrusts the slaughterhouse to slaughter.
5. evaluation of meat quality method
Get the longissimus dorsi muscle of experimental group and control group pig, 1% salt poach 10 minutes, section is carried out sensory evaluation from tender degree, succulence, local flavor 3 aspects of meat, and evaluation group is by forming more than 8 people, according to double-blind evaluation.Every index full marks are 100 minutes, and the higher representation quality of mark is higher, the contrast of averaging.
6. results and discussions
Table 2
Assessment item Experimental group Control group
Tender degree ??85.9 ??85.5
Succulence ??84.8 ??81.6
Local flavor ??87.6 ??80.3
By table 2 result as seen, the tender degree index of experimental group and control group difference are little, and obviously are better than control group in succulence and local flavor, illustrate, adopt interpolation probiotic bacterium of the present invention to cultivate and can obviously improve meat quality.
Embodiment 3
1. the preparation of probiotics leaven:
Adopt the bacterium powder of freeze-drying, survey viable count, prepare probiotics leaven according to the viable count ratio according to table 3.
Table 3
Starter 1 Starter 2 Starter 3 Starter 4 Starter 5 Starter 6
Plant lactobacillus (hundred million/g) ??20 ??2 ??10 ??5 ??5 ??10
Enterococcus faecalis (hundred million/g) ??20 ??2 ??10 ??5 ??5 ??10
Lactobacterium acidophilum (hundred million/g) ??5 ??10 ??10 ??25 ??1 ??20
Bifidobacterium adolescentis (hundred million/g) ??5 ??10 ??10 ??25 ??1 ??20
Yeast (hundred million/g) ??5 ??20 ??20 ??1 ??50 ??20
2. the preparation of probiotics bacterial liquid
Preparation MRS substratum 20L adds the 10g starter, and 35 ℃ of sealed fermentings 36 hours are standby.
3. culture experiment
30 2100 of age in days Qingyuan City chickens are divided into 7 groups, 300 every group at random, 6 groups of normal diets and account for forage volume 1% (massfraction) probiotics bacterial liquid of feeding wherein, feed normal diet in contrast for 1 group, and cultivation is got at random 3 for every group and is slaughtered rear standby to 115 ages in days.
4. evaluation of meat quality method
According to the cold chicken way, the broiler chicken of experimental group and control group is processed respectively, carried out sensory evaluation from tender degree, succulence, local flavor 3 aspects of meat, evaluation group is by forming more than 8 people, according to double-blind evaluation.Every index full marks are 100 minutes, and the higher representation quality of mark is higher, the contrast of averaging.
5. results and discussions
Table 4
Starter 1 Starter 2 Starter 3 Starter 4 Starter 5 Starter 6 Control group
Tender degree ??93.9 ??93.3 ??94.2 ??94.1 ??93.8 ??93.6 ??93.3
Succulence ??94.5 ??93.5 ??95.3 ??94.5 ??93.6 ??95.2 ??91.3
Local flavor ??90.8 ??90.3 ??91.9 ??91.1 ??90.8 ??90.3 ??89.6
By table 4 result as seen, the experimental group indices all makes moderate progress, and illustrates, adopts interpolation probiotic bacterium of the present invention to carry out broiler breeding and can obviously improve meat quality.

Claims (4)

1. an animal health probiotics leaven that improves meat, is characterized in that, comprises A, B and C three components, and the viable count ratio in component A, B, C is 1:(0.2~5): (0.1~5); Described component A is the composition of plant lactobacillus and enterococcus faecalis; Described B component is the composition of Lactobacterium acidophilum and bifidus bacillus; Described component C is S. cervisiae.
2. starter according to claim 1, is characterized in that, in described component A, B and C, the viable count ratio is 1:(0.5~3): (1~2).
3. starter according to claim 1 and 2, is characterized in that, the blending ratio of described component A plant lactobacillus and enterococcus faecalis viable count is 1:(1~2); The blending ratio of described B component Lactobacterium acidophilum and the number of active bifid bacteria is 1:(1~2).
4. starter according to claim 3, is characterized in that, described bifidus bacillus is one or more the composition in baby's bifid, youth bifid, long bifid, short bifid, not tally bifid and angle bifidus bacillus.
CN2011102790858A 2011-09-20 2011-09-20 Animal health care probiotics leavening capable of improving meat quality and application thereof CN102356816B (en)

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CN103146603B (en) * 2013-02-26 2014-12-03 武汉施瑞福生物技术有限公司 Lactic acid bacteria solid co-fermenting method, obtained bacterial agent and use of bacterial agent
CN103416584B (en) * 2013-08-20 2015-04-15 沈阳科丰牧业科技有限公司 Method for improving broiler chicken quality by microecologics
CN103416585B (en) * 2013-08-20 2015-09-30 沈阳科丰牧业科技有限公司 Broiler chicken probiotics is utilized to improve the method for meat chicken production performance and chicken meat quality
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