CN103907749A - Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof - Google Patents
Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof Download PDFInfo
- Publication number
- CN103907749A CN103907749A CN201410148294.2A CN201410148294A CN103907749A CN 103907749 A CN103907749 A CN 103907749A CN 201410148294 A CN201410148294 A CN 201410148294A CN 103907749 A CN103907749 A CN 103907749A
- Authority
- CN
- China
- Prior art keywords
- preparation
- biofermentation
- active carrier
- vitamin
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides biological fermentation active carrier type composite premix feed as well as a preparation method and an application thereof. The preparation method comprises the following steps: preparing probiotics biological fermentation active carriers, to be specific, respectively carrying out liquid fermentation cultivation by using lactobacillus acidophilus LA0912, enterococcus faecalis EF1109, bacillus subtilis BS0903 and saccharomyces cerevisiae SC1203, mixing obtained liquid according to a volume ratio being 1: 1: 1: 1, then carrying out solid fermentation cultivation, drying an obtained solid fermentation culture at lower temperature and grinding so as to obtain the biological fermentation active carriers; compounding the biological fermentation active carriers with vitamins, amino acids, minerals, organic trace elements, choline chloride and table salt so as to obtain the biological fermentation active carrier type composite premix feed suitable for pigs at different stages. The biological fermentation active carrier type composite premix feed gives play to the health function of probiotics, makes the best of multiple biological activity effects of probiotics fermentation metabolites and can meet the physiologic nutritional requirements of pigs at different stages.
Description
Technical field
The present invention relates to microorganism fermented animal food technical field, particularly a kind of biofermentation active carrier type compound premixed feed and its preparation method and application.
Background technology
For many years, due to the complexity of livestock and poultry and Modern Animal Husbandry economized produce prophyiaxis and promoting growth needs, in animal and fowl fodder, add the phenomenon ubiquity of antibiotics.But, in feed, the long-term antibiotics that uses can produce serious negative effect, directly or indirectly threaten the healthy of the mankind, main manifestations is that the resistance to the action of a drug of deterioration, the pathogen of cultivation internal and external environment increases, antibacterials lost efficacy, the immunity degradation of humans and animals etc.In recent years, the appearance of the products such as enzyme preparation, plant extracts, acidulant, genetic engineering antibiotic peptides, the use that reduces antibiotics has been played to certain facilitation, but this series products still fails to make complete feed or premixed feed to break away from the dependence to antibiotics.Along with the development of modern bioscience technology, do not add antibiotic probiotics, fermentative feedstuff of microbe because its security, validity have been considered to be hopeful to develop into a class novel green feed addictive and feed applications in livestock breeding industry.But because the series of technical such as screening and zymotechnique of microorganism fungus kind makes the production of probiotics, fermentative feedstuff of microbe and uses that still to have problems to have to be solved.
Summary of the invention
The technical problem solving: in order to promote digesting and absorbing of nutriment in feed, improve the production performance and the ability of resisting disease of pig, reduce the use of antibiotics, the invention provides a kind of biofermentation active carrier type compound premixed feed and its preparation method and application.Biofermentation active carrier type compound premixed feed of the present invention does not add any antibiotics, and biofermentation active carrier and multivitamin, amino acid, mineral matter, organic trace element, Choline Chloride that the viable bacteria being produced by liquid, solid secondary fermentation by four kinds of probios and metabolite thereof are made, salt is composite forms.This biofermentation active carrier type compound premixed feed had both been brought into play the health care of probio, take full advantage of again the multiple biological action of probiotics fermention metabolite, not only can meet the physiological nutritional need of different phase pig, can also improve its immunity, antagonism pathogenic microorganisms, improve inside and outside ecological environment, improve feed and digest and absorb rate, improve the production performance of pig.
Technical scheme: a kind of biofermentation active carrier type compound premixed feed, prepare and obtain by following steps:
(1) preparation of probio biofermentation active carrier: by lactobacillus acidophilus (Lactobacillus acidophilus) LA0912(CCTCC M2013577), enterococcus faecalis (Enterococcus faecalis) EF1109(CCTCC M2013576), bacillus subtilis (Bacillus subtilis) BS0903(CCTCC M2012078), saccharomyces cerevisiae (Saccharomyces cerevisiae) SC1203(CCTCC M2013578) difference liquid fermentation and culture, the fermentation culture obtaining by volume 1:1:1:1 carries out solid fermentation cultivation after mixing, the solid fermentation culture obtaining is through low temperature drying, after pulverizing as the masterbatch of biofermentation active carrier type compound premixed feed, be called for short biofermentation active carrier,
(2) obtain with the composite vitamin of biofermentation active carrier, amino acid, mineral matter, organic trace element, Choline Chloride and salt the biofermentation active carrier type compound premixed feed that is applicable to different phase swinery.
A kind of biofermentation active carrier type compound premixed feed and preparation method thereof, production craft step is:
1, the preparation of probio biofermentation active carrier: by lactobacillus acidophilus (Lactobacillus acidophilus) LA0912(CCTCC M2013577), enterococcus faecalis (Enterococcus faecalis) EF1109(CCTCC M2013576), bacillus subtilis (Bacillus subtilis) BS0903(CCTCC M2012078), saccharomyces cerevisiae (Saccharomyces cerevisiae) SC1203(CCTCC M2013578) difference liquid fermentation and culture, the fermentation culture obtaining by volume 1:1:1:1 carries out solid fermentation cultivation after mixing, the solid fermentation culture obtaining is through low temperature drying, after pulverizing as the masterbatch of biofermentation active carrier type compound premixed feed, be called for short biofermentation active carrier, 2, obtain with the composite vitamin of biofermentation active carrier, amino acid, mineral matter, organic trace element, Choline Chloride and salt the biofermentation active carrier type compound premixed feed that is applicable to different phase swinery.
The concrete preparation process of described biofermentation active carrier is:
(1) liquid fermentation
1. the preparation of lactobacillus acidophilus LA0912 bacterium liquid: the lactobacillus acidophilus LA0912 bacterial classification of preservation is seeded in the anaerobism pipe that 20mLMRS culture medium is housed, after 37 DEG C of constant temperature culture 20h, be lactobacillus acidophilus seed liquor, again by the seed liquor preparing by volume the inoculum concentration of 1%-10% carry out secondary cultivation, after every grade of 37 DEG C of constant temperature culture 20h, obtain for the lactobacillus acidophilus bacterium liquid of solid fermentation;
Described MRS culture medium prescription is as follows: peptone, 10.0g/L; Yeast extract, 4.0g/L; Beef extract, 8.0g/L; Glucose, 20.0g/L; Tween 80,1mL/L; Sodium acetate trihydrate, 5.0g/L; Dipotassium hydrogen phosphate, 2.0g/L; Triammonium citrate, 2.0g/L; Epsom salt, 0.2g/L; Manganese sulfate, 0.05g/L; PH6.2 ± 0.2;
2. the preparation of enterococcus faecalis EF1109 bacterium liquid: the enterococcus faecalis EF1109 bacterial classification of preservation is seeded in the conical flask of YPD fluid nutrient medium that 100mL is housed, 37 DEG C of constant temperature leave standstill cultivation 20h and are enterococcus faecalis seed liquor, again by the seed liquor preparing by volume the inoculum concentration of 1%-10% carry out secondary cultivation, after every grade of 37 DEG C of standing constant temperature culture 10-20h, obtain for the enterococcus faecalis bacterium liquid of solid fermentation;
Described YPD Liquid Culture based formulas is as follows: peptone, 20.0g/L; Yeast soaks powder, 10.0g/L; Glucose, 20.0g/L; PH6.5 ± 0.2;
3. the preparation of bacillus subtilis BS0903 bacterium liquid: the bacillus subtilis BS0903 bacterial classification of preservation is seeded in the conical flask of MNR culture medium that 100mL is housed, 30 DEG C, shaking speed are to be Bacillus subtillis seed liquor after 225rpm, constant temperature culture 16h, again by the seed liquor preparing by volume the inoculum concentration of 1%-6% carry out secondary cultivation, after every grade of 37 DEG C of standing constant temperature culture 10-20h, obtain for the bacillus subtilis bacterium liquid of solid fermentation;
Described MNB culture medium prescription is as follows: peptone, 5.0g/L; Beef extract, 3.0g/L; Yeast soaks powder, 1.0g/L; Glucose, 5.0g/L; Epsom salt, 0.5g/L; Manganese sulfate, 0.005g/L; PH7.0 ± 0.2;
4. the preparation of Saccharomyces Cerevisiae in S C1203 bacterium liquid: the Saccharomyces Cerevisiae in S C1203 bacterial classification of preservation is seeded in the conical flask of YPD fluid nutrient medium that 100mL is housed, be after 30 DEG C of constant temperature culture 16h, to be S. cervisiae seed liquor in 250rpm shaking table at rotating speed, again by the seed liquor preparing by volume the inoculum concentration of 1%-3% carry out secondary cultivation, shaking speed is after 250rpm, 30 DEG C of constant temperature culture 16h, to obtain for the S. cervisiae liquid of solid fermentation;
Described YPD Liquid Culture based formulas is as follows: peptone, 10.0g/L; Yeast soaks powder, 5.0g/L; Glucose, 20.0g/L; Sodium propionate, 2.5g/L; PH4.0 ± 0.1;
5. the preparation of compound bacteria bacterium liquid: 1:1:1:1 proportioning measures lactobacillus acidophilus LA0912 bacterium liquid, enterococcus faecalis EF1109 bacterium liquid, bacillus subtilis BS0903 bacterium liquid and Saccharomyces Cerevisiae in S C1203 bacterium liquid by volume, mixes and obtains compound bacteria bacterium liquid;
(2) solid fermentation
1. the preparation of solid fermentation culture medium: dregs of beans 22%, corn protein powder 25%, rice bran meal 23%, wheat bran 25%, brown sugar 5% preparation mix in mass ratio;
2. the condition that solid fermentation is cultivated: the inoculum concentration of compound bacteria bacterium liquid in solid fermentation culture medium by volume mass ratio is 3-6% (mL/g), the addition of protease is 100-120U/g, the ratio of water and solid fermentation culture medium is mass ratio 1:1-0.4:1 (mL/g) by volume, compound bacteria bacterium liquid, protease and solid fermentation culture medium mix after fermentation, fermentation temperature 35-40 DEG C, fermentation time is 72-120h;
3. the oven dry of solid fermentation product is pulverized: above-mentioned obtained solid fermentation culture is the required biofermentation active carrier of preparation biofermentation active carrier type compound premixed feed after 40-60 DEG C of low temperature drying, pulverizing.
The preparation method of described biofermentation active carrier type compound premixed feed is: every kilogram of biological fermentation activity carrier model compound premixed feed contains: copper, 200-360mg; Iron, 600-1200mg; Zinc, 800mg; Manganese, 120mg; Iodine, 1.4mg; Selenium, 1mg; Vitamin A, 40000-60000IU; Neo dohyfral D3,10000-15000IU; Vitamin E, 120-200IU; Prokayvit, 8-16mg; Vitamin B1,10-20mg; Vitamin B2,20-40mg; Pyridoxamine, 12mg; Cobalamin, 0.12-0.32mg; Nicotinic acid, 200mg; Folic acid, 4-6mg; Pantothenic acid 100mg; Biotin, 0-1mg; Choline Chloride, 2000mg; Lysine, 0.4-2.8g; Calcium, 40-80g; Total phosphorus, 15g; Salt, 30-60g.
(1) the preparation method of newborn piglet biofermentation active carrier type compound premixed feed is: every kilogram of biological fermentation activity carrier model compound premixed feed contains: copper, 360mg; Iron, 1200mg; Zinc, 800mg; Manganese, 120mg; Iodine, 1.4mg; Selenium, 1mg; Vitamin A, 60000IU; Neo dohyfral D3,15000IU; Vitamin E, 160IU; Prokayvit, 16mg; Vitamin B1,20mg; Vitamin B2,20mg; Pyridoxamine, 12mg; Cobalamin, 0.12mg; Nicotinic acid, 200mg; Folic acid, 6mg; Pantothenic acid 100mg; Choline Chloride, 2000mg; Lysine, 2.8g; Calcium, 65g; Total phosphorus, 15g; Salt, 30g.
(2) the preparation method of growing-finishing pig biofermentation active carrier type compound premixed feed is: every kilogram of biological fermentation activity carrier model compound premixed feed contains: copper, 240mg; Iron, 600mg; Zinc, 800mg; Manganese, 120mg; Iodine, 1.4mg; Selenium, 1mg; Vitamin A, 40000IU; Neo dohyfral D3,10000IU; Vitamin E, 120IU; Prokayvit, 8mg; Vitamin B1,20mg; Vitamin B2,20mg; Pyridoxamine, 12mg; Cobalamin, 0.08mg; Nicotinic acid, 200mg; Pantothenic acid, 100mg; Folic acid, 4mg; Choline Chloride, 2000mg.Lysine, 2.4g; Calcium, 58g; Total phosphorus, 15g; Salt, 30g.
(3) the preparation method of in-pig biofermentation active carrier type compound premixed feed is: every kilogram of biological fermentation activity carrier model compound premixed feed contains: copper, 200mg; Iron, 1200mg; Zinc, 800mg; Manganese, 120mg; Iodine, 1.4mg; Selenium, 1mg; Vitamin A, 50000IU; Neo dohyfral D3,15000IU; Vitamin E, 200IU; Prokayvit, 8mg; Vitamin B1,10mg; Vitamin B2,40mg; Pyridoxamine, 12mg; Cobalamin, 0.32mg; Nicotinic acid, 200mg; Pantothenic acid, 100mg; Folic acid, 6mg; Biotin, 1mg; Choline Chloride, 2000mg; Lysine, 0.4g; Calcium, 65g; Total phosphorus, 15g; Salt, 30g.
(4) the preparation method of milking sow biofermentation active carrier type compound premixed feed is: every kilogram of biological fermentation activity carrier model compound premixed feed contains: copper, 200mg; Iron, 1200mg; Zinc, 800mg; Manganese, 120mg; Iodine, 1.4mg; Selenium, 1mg; Vitamin A, 50000IU; Neo dohyfral D3,15000IU; Vitamin E, 200IU; Prokayvit, 8mg; Vitamin B1,10mg; Vitamin B2,40mg; Pyridoxamine, 12mg; Cobalamin, 0.32mg; Nicotinic acid, 200mg; Pantothenic acid, 100mg; Folic acid, 60mg; Biotin, 1mg; Choline Chloride, 2000mg.Lysine, 1.6g; Calcium, 68g; Total phosphorus, 15g; Salt, 30g.
Arbitrary described method preparation and the application of biofermentation active carrier type compound premixed feed on pig-breeding.
Biofermentation active carrier type compound premixed feed accounts for 10% of preparation pig complete feed weight.
The lactobacillus acidophilus LA0912(Lactobacillus acidophilus LA0912 that the present invention is used) screen in numerous bacterial strains of separating from chitling road for applicant and obtain, be preserved in Chinese Typical Representative culture collection center on November 19th, 2013, address is Wuhan, China Wuhan University, and deposit number is CCTCC NO:M2013577.This lactobacillus acidophilus can secrete antibiotics material (comprising acidolin, bacillus acidophilus's element, lactein etc.), propagation, the regulating intestinal canal colony balance of energy antagonism pathogenic entero becteria, inhibition enteron aisle undesirable microorganism.Its biological property is as follows: bacillus, two terminal circle.0.6-0.9 × 1.5-6 μ m, with single, become two or short chain to occur; Bacterium colony is coarse, calot.Examine under a microscope, show and be wound around or microvillus filiform, from stretching out like the radiation of shadow Jun Dui center.Maltose, galactolipin, lactose, sucrose, cellobiose, mannobiose, trehalose, melibiose, amarogentin can be assimilated, arabinose, gluconate, mannose can not be assimilated.Do not grow below for 15 DEG C, optimum growth temperature 35-38 DEG C, the most suitable growth pH is 5.5-6.0.
The enterococcus faecalis EF1109(Enterococcus faecalis EF1109 that the present invention is used) screen in numerous bacterial strains of separating from animal intestinal for applicant and obtain, be preserved in Chinese Typical Representative culture collection center on November 19th, 2013, address is Wuhan, China Wuhan University, and deposit number is CCTCC NO:M2013576.This bacterium is gram-positive bacteria, well-grown on MRS agar plate, and bacterium colony rounding, smooth, protuberance, canescence are revealed and are dripped a sample, diameter 0.5-1mm; Micro-Microscopic observation, this bacterium is spherical in shape, does not form gemma; 10 DEG C-50 DEG C of growth temperature ranges, pH scope 4-10; Salmonella, enteropathogenic E．Coli and staphylococcus aureus are all had to stronger inhibitory action; Can tolerate the simulated gastric fluid 60min of pH2.5, the highest tolerance gallbladder salinity is 2.0%.
The bacillus subtilis BS0903(Bacillus subtilis BS0903 that the present invention is used) screen in numerous bacterial strains of separating from healthy animal enteron aisle for applicant and obtain, be preserved in Chinese Typical Representative culture collection center on March 14th, 2012, address is Wuhan, China Wuhan University, and deposit number is CCTCC NO:M2012078.Its biological property is as follows: bacterium colony circle, edge indentation, rough surface, opaque, dirty white, gram-positive bacteria, gemma be shaped as oval to column, be positioned at thalline central authorities or slightly inclined to one side, the thalline after formation gemma does not expand.Bacillus subtilis of the present invention can produce the bacteriocin (being mainly fat peptide matters) that salmonella, enteropathogenic E．Coli K88 and staphylococcus aureus is had to strong killing action, also there is the ability of stronger cellulase-producing, can degraded cellulose.Meanwhile, there is feature high temperature resistant, acid and bile tolerance: can tolerate the pelleting temperature of 80 DEG C, the pepsic simulated gastric fluid of 1%pH2.0 and 0.3% artificial cholate.
Saccharomyces Cerevisiae in S C1203(Saccharomyces cerevisiae SC1203 of the present invention) obtain for applicant's separation screening from healthy animal enteron stool, be preserved in Chinese Typical Representative culture collection center on November 19th, 2013, address is Wuhan, China Wuhan University, and deposit number is CCTCC NO:M2013578.Its biological property is as follows: unicellular fungi, be oval, circle or cylindrical, and bacterium colony is thicker, be creamy white, surface wettability, thickness, easily provoked.Saccharomyces cerevisiae of the present invention has good resistance, can be high temperature resistant, acidproof, bile tolerance and tolerance common antibiotics class medicine etc., and can produce the effects such as acid, product enzyme and inhibition pathogen.
Beneficial effect: the ingenious advantage that combines anaerobic bacteria and aerobic bacteria fermentation of the present invention, the specific probio bacterial classification combination of selecting can make lactic acid bacteria breed rapidly, occupy predominance, and the lactobacillus acidophilus that the present invention selects can secrete antibiotics material (comprising acidolin, bacillus acidophilus's element, lactein etc.); Bacillus subtilis can produce the bacteriocin (being mainly fat peptide matters) that salmonella, Escherichia coli and staphylococcus aureus is had to strong killing action, can effectively suppress miscellaneous bacteria, harmful bacteria in solid-state fermentation culture medium, but the growth metabolism impact on the selected saccharomycete of the present invention, lactobacillus acidophilus and enterococcus faecalis is less, can realize solid culture based raw material does not need sterilization just can be directly used in inoculated and cultured; The growth of saccharomyces cerevisiae can be decomposed and utilized carbohydrate synthetic animalcule albumen, improves the content of mycoprotein.Pass through liquid, Gu the biofermentation active component that secondary fermentation obtains is except containing through the probio with excellent characteristics of repeated screening, also containing the not available multiple probiotics fermention metabolite of conventional compound premixed feed, comprise abundant enzyme, organic acid, vitamin, antibacterial peptide class, UGF etc., not only can improve animal immunizing power, antagonism pathogenic microorganisms, improve inside and outside ecological environment, improve feed and digest and absorb rate, can substitute simultaneously or partly substitute the antibiotic in conventional premixed feed formula, phagostimulant, acidulant, enzyme preparation, mould inhibitor etc.The biofermentation active carrier type compound premixed feed product that accounts for preparation pig complete feed 10% of the present invention's design had both retained the function of conventional probiotics probio, avoid again having in conventional probiotics the few shortcoming of probiotics fermention metabolite of critical function, make product except conventional trophism function, also greatly improved immunological regulation and the health care of product; Simultaneously, the designed product of the present invention is easy to use, only needs to be equipped with corn, dregs of beans and wheat bran etc. and can be made into complete feed, has both retained the excellent properties of full price fermented feed, the energy loss that the complete diet pellet of avoiding again fermenting brings, is easy to all kinds of raisers, production Feed Enterprise is used; The biofermentation active carrier type compound premixed feed product preparation of the present invention's design and the interpolation of final products antibiotic-free series products, green safety, is applicable to the use of different phase swinery.
Detailed description of the invention
To illustrate the present invention by the following example, but described embodiment is only for the present invention is described, instead of limits the scope of the invention by any way.
Embodiment 1, liquid fermentation are prepared compound probiotic bacterium liquid
1. the preparation of lactobacillus acidophilus LA0912 bacterium liquid: the lactobacillus acidophilus LA0912 bacterial classification of preservation is seeded in the anaerobism pipe that 20mLMRS culture medium is housed, after 37 DEG C of constant temperature culture 20h, be lactobacillus acidophilus seed liquor, again by the seed liquor preparing by volume the inoculum concentration of 1-10% carry out secondary cultivation, after every grade of 37 DEG C of standing constant temperature culture 20h, obtain for the lactobacillus acidophilus bacterium liquid of solid fermentation;
Described MRS culture medium prescription is as follows: peptone, 10.0g/L; Beef extract, 8.0g/L; Yeast extract, 4.0g/L; Glucose, 20.0g/L; Tween 80,1mL/L; Dipotassium hydrogen phosphate, 2.0g/L; Sodium acetate trihydrate, 5.0g/L; Triammonium citrate, 2.0g/L; Epsom salt, 0.2g/L; Manganese sulfate, 0.05g/L; PH6.2 ± 0.2;
2. the preparation of enterococcus faecalis EF1109 bacterium liquid: the enterococcus faecalis EF1109 bacterial classification of preservation is seeded in the conical flask of YPD fluid nutrient medium that 100mL is housed, 37 DEG C of constant temperature are enterococcus faecalis seed liquor after leaving standstill and cultivating 10-20h, again by the seed liquor preparing by volume the inoculum concentration of 1-10% carry out secondary cultivation, after every grade of 37 DEG C of standing constant temperature culture 10-20h, obtain for the enterococcus faecalis bacterium liquid of solid fermentation;
Described YPD Liquid Culture based formulas is as follows: peptone, 20.0g/L; Yeast soaks powder, 10.0g/L; Glucose, 20.0g/L; PH6.5 ± 0.2;
3. the preparation of bacillus subtilis BS0903 bacterium liquid: the bacillus subtilis BS0903 bacterial classification of preservation is seeded in the conical flask of MNR culture medium that 100mL is housed, 30 DEG C, shaking speed are to be Bacillus subtillis seed liquor after 225rpm, constant temperature culture 16h, again by the seed liquor preparing by volume the inoculum concentration of 1-6% carry out secondary cultivation, every grade of 30 DEG C of constant temperature culture 20h, shaking speed is 225rpm; Cultivation finishes the bacillus subtilis bacterium liquid of rear acquisition for solid fermentation;
Described MNB culture medium prescription is as follows: peptone, 5.0g/L; Beef extract, 3.0g/L; Yeast soaks powder, 1.0g/L; Glucose, 5.0g/L; Epsom salt, 0.5g/L; Manganese sulfate, 0.005g/L; PH7.0 ± 0.2;
4. the preparation of Saccharomyces Cerevisiae in S C1203 bacterium liquid: the Saccharomyces Cerevisiae in S C1203 bacterial classification of preservation is seeded in the conical flask of YPD fluid nutrient medium that 100mL is housed, be after 30 DEG C of constant temperature culture 16h, to be S. cervisiae seed liquor in 250rpm shaking table at rotating speed, again the seed liquor preparing is carried out to secondary cultivation by the inoculum concentration of 1-3%, shaking speed is after 250rpm, 30 DEG C of constant temperature culture 16h, to obtain the S. cervisiae liquid for solid fermentation;
Described YPD Liquid Culture based formulas is as follows: peptone, 10.0g/L; Yeast soaks powder, 5.0g/L; Glucose, 20.0g/L; Sodium propionate, 2.5g/L; PH4.0 ± 0.1;
5. the preparation of compound bacteria bacterium liquid: in four kinds of bacterium, lactobacillus acidophilus concentration is 4.12logCFU/mL, enterococcus faecalis concentration is 4.12logCFU/mL, bacillus subtilis bacteria concentration is 5.45logCFU/mL, is lactobacillus acidophilus according to volume ratio: enterococcus faecalis: bacillus subtilis: saccharomyces cerevisiae=1:1:1:1 mixes and makes compound bacteria bacterium liquid.
Embodiment 2, solid fermentation are prepared biofermentation active carrier
1. the preparation of solid fermentation culture medium: press dregs of beans 22%, corn protein powder 25%, rice bran meal 23%, wheat bran 25%, brown sugar 5% preparation mixing.Described white point of content is weight percentage.
2. the condition that solid fermentation is cultivated: the inoculum concentration of the compound bacteria bacterium liquid that embodiment 1 is made in solid fermentation culture medium is 3-6% (mL/g), the addition of protease is 100-120U/g, the ratio of water and solid fermentation culture medium is by 1 ︰ 1-0.4 ︰ 1 (mL/g), compound bacteria bacterium liquid, protease and solid fermentation culture medium mix after fermentation, fermentation temperature 35-40 DEG C, fermentation time is 72-120h.Obtaining optimum fermentation scheme by multi-factor orthogonal test is: the inoculum concentration of compound bacteria bacterium liquid in solid medium is 4%(mL/g), the addition of protease is 110U/g, the ratio of water and solid medium is by 1 ︰ 0.6(mL/g), compound bacteria bacterium liquid, protease and culture medium mix after fermentation, 37 DEG C of fermentation temperatures, fermentation time is 84h.
3. the oven dry of solid fermentation product is pulverized: above-mentioned obtained solid fermentation culture is the biofermentation active carrier in preparation biofermentation active carrier type compound premixed feed after low temperature drying, pulverizing.
The preparation of embodiment 3, newborn piglet biofermentation active carrier type compound premixed feed
The probiotics fermention bioactive carrier being obtained with embodiment 2 and multivitamin, amino acid, organic trace element, mineral matter, Choline Chloride, salt is composite, evenly mix, and obtains newborn piglet biofermentation active carrier type compound premixed feed.Every kilogram of newborn piglet biofermentation active carrier type compound premixed feed of the present invention contains: copper, 360mg; Iron, 1200mg; Zinc, 800mg; Manganese, 120mg; Iodine, 1.4mg; Selenium, 1mg; Vitamin A, 60000IU; Neo dohyfral D3,15000IU; Vitamin E, 160IU; Prokayvit, 16mg; Vitamin B1,20mg; Vitamin B2,20mg; Pyridoxamine, 12mg; Cobalamin, 0.12mg; Nicotinic acid, 200mg; Folic acid, 6mg; Pantothenic acid 100mg; Choline Chloride, 2000mg; Lysine, 2.8g; Calcium, 65g; Total phosphorus, 15g; Salt, 30g.
The preparation of embodiment 4, growing-finishing pig biofermentation active carrier type compound premixed feed
The probiotics fermention bioactive carrier being obtained with embodiment 2 and multivitamin, amino acid, organic trace element, mineral matter, Choline Chloride, salt is composite, evenly mix, and obtains growing-finishing pig biofermentation active carrier type compound premixed feed.Every kilogram of growing-finishing pig biofermentation active carrier type compound premixed feed of the present invention contains: copper, 240mg; Iron, 600mg; Zinc, 800mg; Manganese, 120mg; Iodine, 1.4mg; Selenium, 1mg; Vitamin A, 40000IU; Neo dohyfral D3,10000IU; Vitamin E, 120IU; Prokayvit, 8mg; Vitamin B1,20mg; Vitamin B2,20mg; Pyridoxamine, 12mg; Cobalamin, 0.08mg; Nicotinic acid, 200mg; Pantothenic acid, 100mg; Folic acid, 4mg; Choline Chloride, 2000mg.Lysine, 2.4g; Calcium, 58g; Total phosphorus, 15g; Salt, 30g.
The preparation of embodiment 5, in-pig biofermentation active carrier type compound premixed feed
The probiotics fermention bioactive carrier being obtained with embodiment 2 and multivitamin, amino acid, organic trace element, mineral matter, Choline Chloride, salt is composite, evenly mix, and obtains in-pig biofermentation active carrier type compound premixed feed.Every kilogram of in-pig biofermentation active carrier type compound premixed feed of the present invention contains: copper, 200mg; Iron, 1200mg; Zinc, 800mg; Manganese, 120mg; Iodine, 1.4mg; Selenium, 1mg; Vitamin A, 50000IU; Neo dohyfral D3,15000IU; Vitamin E, 200IU; Prokayvit, 8mg; Vitamin B1,10mg; Vitamin B2,40mg; Pyridoxamine, 12mg; Cobalamin, 0.32mg; Nicotinic acid, 200mg; Pantothenic acid, 100mg; Folic acid, 6mg; Biotin, 1mg; Choline Chloride, 2000mg; Lysine, 0.4g; Calcium, 65g; Total phosphorus, 15g; Salt, 30g.
The preparation of embodiment 6, milking sow biofermentation active carrier type compound premixed feed
Composite with multivitamin, amino acid, organic trace element, mineral matter, Choline Chloride etc. with the probiotics fermention bioactive carrier that embodiment 2 was obtained, evenly mix, obtain milking sow biofermentation active carrier type compound premixed feed.Every kilogram of lactation in-pig biofermentation active carrier type compound premixed feed of the present invention contains: copper, 200mg; Iron, 1200mg; Zinc, 800mg; Manganese, 120mg; Iodine, 1.4mg; Selenium, 1mg; Vitamin A, 50000IU; Neo dohyfral D3,15000IU; Vitamin E, 200IU; Prokayvit, 8mg; Vitamin B1,10mg; Vitamin B2,40mg; Pyridoxamine, 12mg; Cobalamin, 0.32mg; Nicotinic acid, 200mg; Pantothenic acid, 100mg; Folic acid, 60mg; Biotin, 1mg; Choline Chloride, 2000mg.Lysine, 1.6g; Calcium, 68g; Total phosphorus, 15g; Salt, 30g.
Embodiment 7, verify the feeding effect of newborn piglet biofermentation active carrier type compound premixed feed of the present invention to newborn piglet
(1) test method: select 35 about ages in days, health is anosis, kind is consistent, body weight is close, 150 of individual uniform piglets, is divided at random 3 groups, is respectively test group, control group 1 and control group 2.5 weeks experimental periods.Test each group of column home term harmonization, duration of test is respectively organized the equal free choice feeding of pig, automatic water-drinking, and test pig immunity, sterilization etc. are undertaken by pig farm routine.Test group feed is the newborn piglet biofermentation active carrier type compound premixed feed that adds the embodiment of the present invention 3 in Corn-soybean daily ration by 10% content.Control group 1 feed is the 10% piglet compound premixed feed (carrier is defatted rice bran) that adds conventional formulation autogamy in Corn-soybean daily ration.Control group 2 feeds are in Corn-soybean daily ration, to add 10% conventional commercially available piglet compound premixed feed in early stage.
(2) testing index:
Initial weight, heavy, the average daily feed intake in end, Average weight increasing a day, material anharmonic ratio, diarrhea rate.
(3) result of the test:
(4) interpretation of result: as seen from the above table, test group, with respect to control group 1 and the average daily feed intake significant difference of control group 2 (P < 0.05), shows that newborn piglet biofermentation active carrier type compound premixed feed of the present invention can significantly improve the palatability of feed.Test group weightening finish has improved respectively 23.95% and 20.30% than control group 1 and control group 2, significant difference (P < 0.05).Test group is with respect to control group 1 and control group 2 material anharmonic ratioes respectively low 12.20% and 10.37%, significant difference (P < 0.05).Show that newborn piglet biofermentation active carrier type compound premixed feed of the present invention can significantly improve efficiency of feed utilization.Meanwhile, due to the enhancing of pigling immunity, the diarrhea rate of piglet also obviously declines, and the physical condition of piglet improves greatly, from and significantly improved the production performance of piglet.
Embodiment 8, verify the feeding effect of growing-finishing pig biofermentation active carrier type compound premixed feed of the present invention to growing-finishing pig
(1) test method: 120 of the white growing and fattening pigs of length that selection health is anosis, body weight is close (about 65Kg), be divided at random 3 groups, be respectively test group, control group group 1 and control group group 2.8 weeks experimental periods.Test group column home term harmonization, duration of test is respectively organized the equal free choice feeding of pig, automatic water-drinking, and test pig immunity, sterilization etc. are undertaken by pig farm routine.Test group feed is the growing-finishing pig biofermentation active carrier type compound premixed feed that adds the embodiment of the present invention 4 in Corn-soybean daily ration by 10% content.Control group 1 feed is the 10% growing and fattening compound premixed feed (carrier is defatted rice bran) that adds conventional formulation autogamy in Corn-soybean daily ration.Control group 2 feeds are in Corn-soybean daily ration, to add conventional commercially available 10% to fatten compound premixed feed in early stage.
(2) testing index:
Average daily feed intake, Average weight increasing a day, material anharmonic ratio.
(3) result of the test:
| Group | A test number | Average daily feed intake (g) | Average weight increasing a day (g) | Material anharmonic ratio | Survival rate (%) |
| Test group | 40 | 3505 | 1120 | 3.13 | 100 |
| Control group 1 | 40 | 3431 | 1066 | 3.22 | 100 |
| Control group 2 | 40 | 3457 | 1077 | 3.21 | 100 |
(4) interpretation of result: as seen from the above table, test group has improved respectively 2.16% and 1.39%, significant difference (P < 0.05) with respect to control group 1 and the average daily feed intake of control group 2.Test group is with respect to control group 1 and control group 2 material anharmonic ratioes respectively low 2.88% and 2.56%, significant difference (P < 0.05).Show that growing-finishing pig biofermentation active carrier type compound premixed feed of the present invention has the effect that significantly improves growth of growing-finishing pigs performance.
Embodiment 9, verify the feeding effect of in-pig biofermentation active carrier type compound premixed feed of the present invention to farrowing sow
(1) test method: select 60 sows that health is anosis, parity is close, be divided at random 2 groups, be respectively test group, control group.Sow two groups of column home term harmonizations period of pregnancy, 3, every circle.Test is bred to first 20 days of the expected date of childbirth hello farrowing sow feed from last tire farrowing wean, within one day, feeds twice, and each 1Kg, freely drinks water.First 20 days of the expected date of childbirth started three groups and changes identical lactation feed into until childbirth.Test pig immunity, sterilization etc. are undertaken by pig farm routine.Test group feed is the in-pig biofermentation active carrier type compound premixed feed that adds the embodiment of the present invention 5 in Corn-soybean-wheat bran daily ration by 10% content.Control group feed is the 10% in-pig compound premixed feed (carrier is defatted rice bran) that adds conventional formulation autogamy in Corn-soybean-wheat bran daily ration.
(2) testing index:
Parturition rate, the equal litter size of nest, the number of live births, average birth weight, prevention of sow constipation head number.
(3) result of the test:
(4) interpretation of result: as seen from the above table, test group has improved 11.53% than contrast group parturition rate, significant difference (P < 0.05), the number of live births has improved 12.61%, significant difference (P < 0.05), average birth weight has improved 7.25%, significant difference (P < 0.05).Test group prevention of sow constipation number is zero.Show that in-pig biofermentation active carrier type compound premixed feed of the present invention can obviously improve production performance and the general level of the health of farrowing sow.
Embodiment 10, verify the feeding effect of milking sow biofermentation active carrier type compound premixed feed of the present invention to milking sow
(1) test method: select identical, healthy, the parity of kind and the expected date of childbirth 60 close farrowing sows, be divided at random 3 groups, be respectively test group, control group 1 and control group 2.Test from the expected date of childbirth 20 days to 21 days postpartum.Test pig raising, immunity, sterilization etc. are undertaken by pig farm routine.Test group feed is the milking sow biofermentation active carrier type compound premixed feed that adds the embodiment of the present invention 6 in Corn-soybean-wheat bran daily ration by 10% content.Control group 1 feed is the 10% milking sow compound premixed feed (carrier is defatted rice bran) that adds conventional formulation autogamy in Corn-soybean-wheat bran daily ration.Control group 2 feeds are in Corn-soybean daily ration, to add 10% conventional commercially available milking sow compound premixed feed.
(2) testing index:
21 age in days sucking pig nests are number of individuals alive, sucking pig individual weight, sucking pig survival rate, the scorching morbidity of Gilt Uterus number all.
(3) result of the test:
(4) interpretation of result: as seen from the above table, test group significantly improves than the individual counterpoise of the sucking pig of 21 ages in days of control group 1 and control group 2,21 age in days sucking pig survival rates and sucking pig daily gain, significant difference (P < 0.05).The scorching morbidity of a test group Gilt Uterus number is zero.Show that in-pig biofermentation active carrier type compound premixed feed of the present invention can meet better the nutritional need of milking sow, improve the sow general level of the health, and promote suckling pig growth and development, raising sucking pig survival rate.
For the technical scheme that the embodiment of the present invention is provided is described in detail, by the above-mentioned explanation to the embodiment of the present invention, be only applicable to help professional and technical personnel in the field can realize or use the present invention above.To those skilled in the art, according to the embodiment of the present invention,, these embodiment are all had to multiple amendment, and realize in other embodiments consistent in the situation that in principle disclosed herein and features of novelty.In sum, the invention process content should not be construed as limitation of the present invention.
Claims (10)
1. a biofermentation active carrier type compound premixed feed, is characterized in that being prepared and being obtained by following steps:
(1) preparation of probio biofermentation active carrier: by lactobacillus acidophilus (Lactobacillus acidophilus) LA0912, enterococcus faecalis (Enterococcus faecalis) EF1109, bacillus subtilis (Bacillus subtilis) BS0903, saccharomyces cerevisiae (Saccharomyces cerevisiae) SC1203 is liquid fermentation and culture respectively, the fermentation culture obtaining by volume 1:1:1:1 carries out solid fermentation cultivation after mixing, the solid fermentation culture obtaining is through low temperature drying, after pulverizing as the masterbatch of biofermentation active carrier type compound premixed feed, be called for short biofermentation active carrier,
(2) obtain with the composite vitamin of biofermentation active carrier, amino acid, mineral matter, organic trace element, Choline Chloride and salt the biofermentation active carrier type compound premixed feed that is applicable to different phase swinery.
2. a preparation method for biofermentation active carrier type compound premixed feed, is characterized in that its production craft step is:
(1) preparation of probio biofermentation active carrier: by lactobacillus acidophilus (Lactobacillus acidophilus) LA0912, enterococcus faecalis (Enterococcus faecalis) EF1109, bacillus subtilis (Bacillus subtilis) BS0903, saccharomyces cerevisiae (Saccharomyces cerevisiae) SC1203 is liquid fermentation and culture respectively, the fermentation culture obtaining by volume 1:1:1:1 carries out solid fermentation cultivation after mixing, the solid fermentation culture obtaining is through low temperature drying, after pulverizing as the masterbatch of biofermentation active carrier type compound premixed feed, be called for short biofermentation active carrier,
(2) obtain with the composite vitamin of biofermentation active carrier, amino acid, mineral matter, organic trace element, Choline Chloride and salt the biofermentation active carrier type compound premixed feed that is applicable to different phase swinery.
3. the preparation method of biofermentation active carrier type compound premixed feed according to claim 2, is characterized in that the concrete preparation process of described biofermentation active carrier is:
(1) liquid fermentation
1. the preparation of lactobacillus acidophilus LA0912 bacterium liquid: the lactobacillus acidophilus LA0912 bacterial classification of preservation is seeded in the anaerobism pipe that 20mLMRS culture medium is housed, after 37 DEG C of constant temperature culture 20h, be lactobacillus acidophilus seed liquor, again by the seed liquor preparing by volume the inoculum concentration of 1%-10% carry out secondary cultivation, after every grade of 37 DEG C of constant temperature culture 20h, obtain for the lactobacillus acidophilus bacterium liquid of solid fermentation;
Described MRS culture medium prescription is as follows: peptone, 10.0g/L; Yeast extract, 4.0g/L; Beef extract, 8.0g/L; Glucose, 20.0g/L; Tween 80,1mL/L; Sodium acetate trihydrate, 5.0g/L; Dipotassium hydrogen phosphate, 2.0g/L; Triammonium citrate, 2.0g/L; Epsom salt, 0.2g/L; Manganese sulfate, 0.05g/L; PH6.2 ± 0.2;
2. the preparation of enterococcus faecalis EF1109 bacterium liquid: the enterococcus faecalis EF1109 bacterial classification of preservation is seeded in the conical flask of YPD fluid nutrient medium that 100mL is housed, 37 DEG C of constant temperature leave standstill cultivation 20h and are enterococcus faecalis seed liquor, again by the seed liquor preparing by volume the inoculum concentration of 1%-10% carry out secondary cultivation, after every grade of 37 DEG C of standing constant temperature culture 10-20h, obtain for the enterococcus faecalis bacterium liquid of solid fermentation;
Described YPD Liquid Culture based formulas is as follows: peptone, 20.0g/L; Yeast soaks powder, 10.0g/L; Glucose, 20.0g/L; PH6.5 ± 0.2;
3. the preparation of bacillus subtilis BS0903 bacterium liquid: the bacillus subtilis BS0903 bacterial classification of preservation is seeded in the conical flask of MNR culture medium that 100mL is housed, 30 DEG C, shaking speed are to be Bacillus subtillis seed liquor after 225rpm, constant temperature culture 16h, again by the seed liquor preparing by volume the inoculum concentration of 1%-6% carry out secondary cultivation, after every grade of 37 DEG C of standing constant temperature culture 10-20h, obtain for the bacillus subtilis bacterium liquid of solid fermentation;
Described MNB culture medium prescription is as follows: peptone, 5.0g/L; Beef extract, 3.0g/L; Yeast soaks powder, 1.0g/L; Glucose, 5.0g/L; Epsom salt, 0.5g/L; Manganese sulfate, 0.005g/L; PH7.0 ± 0.2;
4. the preparation of Saccharomyces Cerevisiae in S C1203 bacterium liquid: the Saccharomyces Cerevisiae in S C1203 bacterial classification of preservation is seeded in the conical flask of YPD fluid nutrient medium that 100mL is housed, be after 30 DEG C of constant temperature culture 16h, to be S. cervisiae seed liquor in 250rpm shaking table at rotating speed, again by the seed liquor preparing by volume the inoculum concentration of 1%-3% carry out secondary cultivation, shaking speed is after 250rpm, 30 DEG C of constant temperature culture 16h, to obtain for the S. cervisiae liquid of solid fermentation;
Described YPD Liquid Culture based formulas is as follows: peptone, 10.0g/L; Yeast soaks powder, 5.0g/L; Glucose, 20.0g/L; Sodium propionate, 2.5g/L; PH4.0 ± 0.1;
5. the preparation of compound bacteria bacterium liquid: 1:1:1:1 proportioning measures lactobacillus acidophilus LA0912 bacterium liquid, enterococcus faecalis EF1109 bacterium liquid, bacillus subtilis BS0903 bacterium liquid and Saccharomyces Cerevisiae in S C1203 bacterium liquid by volume, mixes and obtains compound bacteria bacterium liquid;
(2) solid fermentation
1. the preparation of solid fermentation culture medium: dregs of beans 22%, corn protein powder 25%, rice bran meal 23%, wheat bran 25%, brown sugar 5% preparation mix in mass ratio;
2. the condition that solid fermentation is cultivated: the inoculum concentration of compound bacteria bacterium liquid in solid fermentation culture medium by volume mass ratio is 3-6% (mL/g), the addition of protease is 100-120U/g, the ratio of water and solid fermentation culture medium is mass ratio 1:1-0.4:1 (mL/g) by volume, compound bacteria bacterium liquid, protease and solid fermentation culture medium mix after fermentation, fermentation temperature 35-40 DEG C, fermentation time is 72-120h;
3. the oven dry of solid fermentation product is pulverized: above-mentioned obtained solid fermentation culture is the required biofermentation active carrier of preparation biofermentation active carrier type compound premixed feed after 40-60 DEG C of low temperature drying, pulverizing.
4. the preparation method of biofermentation active carrier type compound premixed feed according to claim 2, is characterized in that preparation method is: every kilogram of biological fermentation activity carrier model compound premixed feed contains: copper, 200-360mg; Iron, 600-1200mg; Zinc, 800mg; Manganese, 120mg; Iodine, 1.4mg; Selenium, 1mg; Vitamin A, 40000-60000IU; Neo dohyfral D3,10000-15000IU; Vitamin E, 120-200IU; Prokayvit, 8-16mg; Vitamin B1,10-20mg; Vitamin B2,20-40mg; Pyridoxamine, 12mg; Cobalamin, 0.12-0.32mg; Nicotinic acid, 200mg; Folic acid, 4-6mg; Pantothenic acid 100mg; Biotin, 0-1mg; Choline Chloride, 2000mg; Lysine, 0.4-2.8g; Calcium, 40-80g; Total phosphorus, 15g; Salt, 30-60g.
5. the preparation method of biofermentation active carrier type compound premixed feed according to claim 2, is characterized in that preparation method is: every kilogram of biological fermentation activity carrier model compound premixed feed contains: copper, 360mg; Iron, 1200mg; Zinc, 800mg; Manganese, 120mg; Iodine, 1.4mg; Selenium, 1mg; Vitamin A, 60000IU; Neo dohyfral D3,15000IU; Vitamin E, 160IU; Prokayvit, 16mg; Vitamin B1,20mg; Vitamin B2,20mg; Pyridoxamine, 12mg; Cobalamin, 0.12mg; Nicotinic acid, 200mg; Folic acid, 6mg; Pantothenic acid 100mg; Choline Chloride, 2000mg; Lysine, 2.8g; Calcium, 65g; Total phosphorus, 15g; Salt, 30g.
6. the preparation method of biofermentation active carrier type compound premixed feed according to claim 2, is characterized in that preparation method is: every kilogram of biological fermentation activity carrier model compound premixed feed contains: copper, 240mg; Iron, 600mg; Zinc, 800mg; Manganese, 120mg; Iodine, 1.4mg; Selenium, 1mg; Vitamin A, 40000IU; Neo dohyfral D3,10000IU; Vitamin E, 120IU; Prokayvit, 8mg; Vitamin B1,20mg; Vitamin B2,20mg; Pyridoxamine, 12mg; Cobalamin, 0.08mg; Nicotinic acid, 200mg; Pantothenic acid, 100mg; Folic acid, 4mg; Choline Chloride, 2000mg.Lysine, 2.4g; Calcium, 58g; Total phosphorus, 15g; Salt, 30g.
7. the preparation method of biofermentation active carrier type compound premixed feed according to claim 2, is characterized in that preparation method is: every kilogram of biological fermentation activity carrier model compound premixed feed contains: copper, 200mg; Iron, 1200mg; Zinc, 800mg; Manganese, 120mg; Iodine, 1.4mg; Selenium, 1mg; Vitamin A, 50000IU; Neo dohyfral D3,15000IU; Vitamin E, 200IU; Prokayvit, 8mg; Vitamin B1,10mg; Vitamin B2,40mg; Pyridoxamine, 12mg; Cobalamin, 0.32mg; Nicotinic acid, 200mg; Pantothenic acid, 100mg; Folic acid, 6mg; Biotin, 1mg; Choline Chloride, 2000mg; Lysine, 0.4g; Calcium, 65g; Total phosphorus, 15g; Salt, 30g.
8. the preparation method of biofermentation active carrier type compound premixed feed according to claim 2, is characterized in that preparation method is: every kilogram of biological fermentation activity carrier model compound premixed feed contains: copper, 200mg; Iron, 1200mg; Zinc, 800mg; Manganese, 120mg; Iodine, 1.4mg; Selenium, 1mg; Vitamin A, 50000IU; Neo dohyfral D3,15000IU; Vitamin E, 200IU; Prokayvit, 8mg; Vitamin B1,10mg; Vitamin B2,40mg; Pyridoxamine, 12mg; Cobalamin, 0.32mg; Nicotinic acid, 200mg; Pantothenic acid, 100mg; Folic acid, 60mg; Biotin, 1mg; Choline Chloride, 2000mg.Lysine, 1.6g; Calcium, 68g; Total phosphorus, 15g; Salt, 30g.
The arbitrary described method preparation of claim 2～9 and the application of biofermentation active carrier type compound premixed feed on pig-breeding.
10. the application of biofermentation active carrier type compound premixed feed on pig-breeding according to claim 9, is characterized in that: biofermentation active carrier type compound premixed feed accounts for 10% of preparation pig complete feed weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410148294.2A CN103907749A (en) | 2014-04-10 | 2014-04-10 | Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410148294.2A CN103907749A (en) | 2014-04-10 | 2014-04-10 | Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103907749A true CN103907749A (en) | 2014-07-09 |
Family
ID=51034019
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410148294.2A Pending CN103907749A (en) | 2014-04-10 | 2014-04-10 | Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103907749A (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104351544A (en) * | 2014-11-11 | 2015-02-18 | 河南旭瑞饲料有限公司 | Compound premix based on coating technology and production method thereof |
| CN105918666A (en) * | 2016-04-25 | 2016-09-07 | 安徽和盛伟业饲料有限公司 | Compound premix for large pigs |
| CN106720940A (en) * | 2016-12-07 | 2017-05-31 | 天津牧丰饲料有限公司 | Multiple-microorganism fermentation meat-chicken complete feedstuff and its preparation method and application |
| CN106721278A (en) * | 2016-12-07 | 2017-05-31 | 天津牧丰饲料有限公司 | Microbial fermentation child care phase piglet liquid-state feed and preparation method and application |
| CN106929441A (en) * | 2015-12-30 | 2017-07-07 | 湖北华扬科技发展有限公司 | A kind of cultural method of enterococcus faecalis |
| CN107410363A (en) * | 2017-03-23 | 2017-12-01 | 北京百丰天下生物科技有限公司 | New bio control worm agent and preparation method thereof |
| CN108782970A (en) * | 2018-08-02 | 2018-11-13 | 北京绿摇饲料科技有限公司 | One broad sow perfect compound feed |
| CN108947164A (en) * | 2016-01-22 | 2018-12-07 | 浙江佳净洁环境科技有限公司 | A kind of application method of complex microorganism preparations |
| CN109170161A (en) * | 2018-09-03 | 2019-01-11 | 佛山市正典生物技术有限公司 | For improving the premix and preparation method thereof of pig feed intake and production performance |
| CN111903838A (en) * | 2020-08-13 | 2020-11-10 | 厦门惠盈动物科技有限公司 | Yeast culture and compound lactobacillus preparation and preparation method thereof |
| CN112515044A (en) * | 2020-12-01 | 2021-03-19 | 北京挑战农业科技有限公司 | Preparation method and application of fermentation premix capable of enhancing vitamin stability |
| CN116531436A (en) * | 2023-05-19 | 2023-08-04 | 山东健源生物科技有限公司 | Biological agent for ruminant rumen regulation and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR19990079858A (en) * | 1998-04-03 | 1999-11-05 | 이태근 | Disposal of food waste by circulating use of chicken and earthworm |
| CN101580799A (en) * | 2008-05-14 | 2009-11-18 | 北京大北农科技集团股份有限公司 | Micro-ecological preparation and application thereof |
| CN102277325A (en) * | 2011-08-12 | 2011-12-14 | 北京金泰得生物科技股份有限公司 | Enterococcus faecalis strain for feed purpose and use thereof |
| CN102409015A (en) * | 2011-12-06 | 2012-04-11 | 北京大北农科技集团股份有限公司 | Composite micro-ecological preparation as well as premixed material and application of preparation in feed additive |
| CN102499321A (en) * | 2011-10-26 | 2012-06-20 | 北京大北农科技集团股份有限公司 | Nonreactive fermented feed of mixed bacteria and preparation method thereof |
-
2014
- 2014-04-10 CN CN201410148294.2A patent/CN103907749A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR19990079858A (en) * | 1998-04-03 | 1999-11-05 | 이태근 | Disposal of food waste by circulating use of chicken and earthworm |
| CN101580799A (en) * | 2008-05-14 | 2009-11-18 | 北京大北农科技集团股份有限公司 | Micro-ecological preparation and application thereof |
| CN102277325A (en) * | 2011-08-12 | 2011-12-14 | 北京金泰得生物科技股份有限公司 | Enterococcus faecalis strain for feed purpose and use thereof |
| CN102499321A (en) * | 2011-10-26 | 2012-06-20 | 北京大北农科技集团股份有限公司 | Nonreactive fermented feed of mixed bacteria and preparation method thereof |
| CN102409015A (en) * | 2011-12-06 | 2012-04-11 | 北京大北农科技集团股份有限公司 | Composite micro-ecological preparation as well as premixed material and application of preparation in feed additive |
Non-Patent Citations (1)
| Title |
|---|
| 宋良敏: "复合微生态制剂在养猪生产上的应用研究", 《中国优秀硕士学位论文全文数据库》, 15 July 2013 (2013-07-15) * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104351544A (en) * | 2014-11-11 | 2015-02-18 | 河南旭瑞饲料有限公司 | Compound premix based on coating technology and production method thereof |
| CN106929441A (en) * | 2015-12-30 | 2017-07-07 | 湖北华扬科技发展有限公司 | A kind of cultural method of enterococcus faecalis |
| CN108947164A (en) * | 2016-01-22 | 2018-12-07 | 浙江佳净洁环境科技有限公司 | A kind of application method of complex microorganism preparations |
| CN105918666A (en) * | 2016-04-25 | 2016-09-07 | 安徽和盛伟业饲料有限公司 | Compound premix for large pigs |
| CN106720940A (en) * | 2016-12-07 | 2017-05-31 | 天津牧丰饲料有限公司 | Multiple-microorganism fermentation meat-chicken complete feedstuff and its preparation method and application |
| CN106721278A (en) * | 2016-12-07 | 2017-05-31 | 天津牧丰饲料有限公司 | Microbial fermentation child care phase piglet liquid-state feed and preparation method and application |
| CN107410363A (en) * | 2017-03-23 | 2017-12-01 | 北京百丰天下生物科技有限公司 | New bio control worm agent and preparation method thereof |
| CN108782970A (en) * | 2018-08-02 | 2018-11-13 | 北京绿摇饲料科技有限公司 | One broad sow perfect compound feed |
| CN109170161A (en) * | 2018-09-03 | 2019-01-11 | 佛山市正典生物技术有限公司 | For improving the premix and preparation method thereof of pig feed intake and production performance |
| CN111903838A (en) * | 2020-08-13 | 2020-11-10 | 厦门惠盈动物科技有限公司 | Yeast culture and compound lactobacillus preparation and preparation method thereof |
| CN112515044A (en) * | 2020-12-01 | 2021-03-19 | 北京挑战农业科技有限公司 | Preparation method and application of fermentation premix capable of enhancing vitamin stability |
| CN116531436A (en) * | 2023-05-19 | 2023-08-04 | 山东健源生物科技有限公司 | Biological agent for ruminant rumen regulation and preparation method thereof |
| CN116531436B (en) * | 2023-05-19 | 2024-02-06 | 山东健源生物科技有限公司 | Biological agent for ruminant rumen regulation and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103907749A (en) | Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof | |
| CN103598447B (en) | Non-antibiotic sucking pig milk replacer and preparation method | |
| CN102168045B (en) | Bacillus subtilis preparation and preparation method thereof | |
| CN102742728B (en) | Livestock and poultry composite probiotics feed additive and premix, concentrate feed and batch | |
| CN102226162B (en) | Preparation method and application of composite microbial feed additive | |
| CN102326677B (en) | Composite micro-ecological feed additive and preparation method and premix thereof | |
| KR102255611B1 (en) | Method for preparing fermented total mixed ration using microbial strain complex | |
| CN103783315B (en) | A kind of micro-ecological additive and its feed | |
| CN102907563B (en) | Method for preparing high-activity probiotic preparation for livestock breeding | |
| CN104293697A (en) | Chicken feed probiotic agent containing enterococcus faecalis and preparation method of chicken feed probiotic agent | |
| CN102265984A (en) | Feed for early weaned piglets | |
| CN106962609A (en) | A kind of direct putting type biological fermentation feed additive and its production and use | |
| CN101019609A (en) | Solid fermentation process for producing natto bacillus feed additive with bean dregs | |
| CN103966124A (en) | Compound microecological preparation and additive, as well as premix thereof | |
| CN104206789B (en) | It is a kind of to be used to prevent mastitis for milk cows and the feed addictive of brucellosis and preparation method thereof | |
| CN100563671C (en) | Fox immunity enhancer | |
| CN102885202A (en) | Novel poultry micro-ecological preparation and preparation method thereof | |
| CN106721261A (en) | One kind is used for swine rearing mixed fermentation fiber feedstuff and preparation method thereof | |
| CN102965316B (en) | Bacteriocin containing class and the feed addictive of Pediococcus acidilactici, premix and batch | |
| CN105010770A (en) | Lamb creep feed and lamb creep method | |
| CN105994941A (en) | Non-antibiotic feed prepared through microbial fermentation | |
| CN108402320A (en) | A kind of composite probiotics preparations and its application in pig starter feed | |
| CN101971920A (en) | Porcine lactobacillus reuteri lyophilized preparation and preparation method thereof | |
| CN108835444A (en) | Kind goose feed, preparation method, application and fowl food | |
| CN102860411A (en) | Lactobacillus rhamnosus probiotic and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140709 |