CN106721278A - Microbial fermentation child care phase piglet liquid-state feed and preparation method and application - Google Patents
Microbial fermentation child care phase piglet liquid-state feed and preparation method and application Download PDFInfo
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- CN106721278A CN106721278A CN201611114578.5A CN201611114578A CN106721278A CN 106721278 A CN106721278 A CN 106721278A CN 201611114578 A CN201611114578 A CN 201611114578A CN 106721278 A CN106721278 A CN 106721278A
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Abstract
The invention discloses one kind fermentation liquid pig starter feed and preparation method and application.It is by Bacillus, lactobacillus mixed combines；Wherein Bacillus and lactic acid bacteria combined inoculation amount are 4% ~ 6%（v/w）, wherein Bacillus and lactobacillus inoculum ratio are 2:1~3:1（v/v）, Bacillus B1 is 1 with Bacillus B2 inoculative proportions:1（v/v）, lactic acid bacteria R1 and lactic acid bacteria R2 inoculative proportions are 1:2（v/v）, material-water ratio is 1:3~1:3.5（w/w）.Fermentation liquid-state feed prepared by the present invention is improving the utilization rate of feed, improves feed local flavor, and improving the aspects such as palatability, preventing and treating " weanling pig syndrome " has obvious effect.By fermentation, reduced sugar, lactic acid content substantially increase feed, and prebiotic bacterium number substantially increases, with sour fragrance.
Description
Technical field
The invention belongs to fermentative feedstuff of microbe technical field, and in particular to one kind fermentation liquid pig starter feed and its preparation
Method.
Background technology
Weaning Age with piglet constantly shifts to an earlier date, and is faced with the challenge of " early weaning syndrome ", such as cannot be effective
Solution will make early weaning lose expectancy.For a long time, people's custom adds antibiotic to strengthen the immunocompetence of piglet,
The various diseases such as diarrhoea are controlled with this.By extensive and long-term use antibiotic, the negative effect that antibiotic brings is increasingly
It is many.In today that food security receives much attention, the people of other countries disable antibiotic one after another.
Compared with other feeds, fermentation liquid child care phase pig starter feed has following advantage：First, in physical aspect and mother
Breast is similar, and piglet can simultaneously obtain the supply of nutrient and water；Second, the microorganism generation such as substantial amounts of lactic acid is produced in fermentation process
Thank to product, liquid fermentation feed is contained special fragrance, good palatability lures that piglet feed intake increases into, and the speed of growth is accelerated；
3rd, fermentation liquid pig starter feed often contains substantial amounts of probiotics, and it is mainly by consuming oxygen, producing lactic acid and secretion
Some antibacterial materials strengthen piglet immunological function, kill pathogenic microorganism, prevent the generation of the diseases such as grice diarrhoea.
The content of the invention
It is anti-to replace the invention discloses a kind of preparation method and application of microbial fermentation child care phase piglet liquid-state feed
The addition and preventing and treating piglet " early weaning syndrome " of raw element.
Technical scheme disclosed by the invention is as follows：
A kind of liquid bacterium solution for the liquid pig starter feed that ferments, it is characterised in that it be to be combined by Bacillus and lactic acid bacteria and
Into；Bacillus are 2 with the volume parts ratio of lactobacillus inoculum:1~3:1（v/v）, the Bacillus refer to gemma B1 and gemma
Bacterium B2, B1 B2 inoculative proportions are 1:1（v/v）, the lactic acid bacteria refers to：Lactic acid bacteria R1 and lactic acid bacteria R2, R1 and R2 inoculation ratio
Example is 1:2（v/v）；Wherein Bacillus B1 refers to bacillus subtilis（CGMCC 1.504）, Bacillus B2 refer to solve starch
Bacillus（CGMCC3413）, lactic acid bacteria R1 refers to bacillus coagulans（CGMCC1.1322）, lactic acid bacteria R2 refers to excrement
Enterococcus（CGMCC1.130）.
The present invention further discloses a kind of preparation method and application of microbial fermentation child care phase piglet liquid-state feed, its
It is characterised by being carried out by the steps：
1）By lactic acid bacteria in 42 DEG C of quiescent culture 24h of MRS medium liquids, Bacillus are in 35 DEG C, 200 revs/min of LB culture mediums
Clock, concussion and cultivate 18h obtains lactobacillus solution and Bacillus seed liquor；
LB culture mediums（g/L）：Tryptone 10, yeast extract powder 5, NaCl 10, pH7.5.
MRS culture mediums（g/L）：Peptone 10, beef extract 10, yeast extract powder 5, glucose 5, CH3COONa 5, lemon
Acid diamine 2, Tween 80 1, K2HPO42, MgSO4•7H2O 0.2, MnSO4•7H2O 0.05, pH6.2.
2）Vegetable raw material is crushed, is well mixed with water, be heated to 100 DEG C, hold time 10min；
3）Treat that temperature drops to 30 DEG C or so, be inoculated with liquid microorganism seed liquid, Plastic Drum or stainless steel cask are loaded after being well mixed
Interior fermentation；In some embodiments of the invention, in the step 2）In, vegetable raw material grinding particle size reaches material 80% and leads to
Cross 40 mesh.
In some embodiments of the invention, in the step 3）In, vegetable raw material is 1 with water quality ratio:3~1:4.5
（W/V）.
In some embodiments of the invention, in the step 3）A kind of middle liquid bacteria for the liquid pig starter feed that ferments
Liquid, it is characterised in that it is combined by Bacillus and lactic acid bacteria；Wherein Bacillus and lactic acid bacteria combined inoculation amount be 4% ~
6%（v/w）, Bacillus and lactobacillus inoculum ratio are 2:1~3:1（v/v）, Bacillus B1 is 1 with Bacillus B2 inoculative proportions:1
（v/v）, lactic acid bacteria R1 and lactic acid bacteria R2 inoculative proportions are 1:2（v/v）.
Described Bacillus B1 refers to bacillus subtilis（CGMCC 1.504）, Bacillus B2 refer to solve starch bud
Spore bacillus（CGMCC3413）, lactic acid bacteria R1 refers to bacillus coagulans（CGMCC1.1322）, lactic acid bacteria R2 refers to excrement intestines
Coccus（CGMCC1.130）.
In some embodiments of the invention, in the step 3）In, vegetable raw material proportioning is：Corn flour:Dregs of beans=3:
1.In some embodiments of the invention, in the step 2）In, 30 DEG C~35 DEG C of fermentation temperature, fermentation time is 4 days~10
My god, the liquid child care phase pig starter feed that ferments is obtained, the fermented feed content of reducing sugar reaches more than 1.56%, and lactic acid content reaches
More than 4.0%, lactic acid bacteria number reaches 109More than cfu/g, E. coli detection less than.
The present invention is further disclosed for liquid bacterium solution zymotic fluid state pig starter feed, and the fermentation liquid-state feed can be improved
The utilization rate of pig starter feed, improves feed local flavor, and improve preventing and treating weanling pig syndrome.Experimental result shows:Zymotic fluid
State pig starter feed increased feed digestion absorption efficiency, and daily gain improves 11.68%；And the probiotics in fermented feed improves
The micro-ecological environment of enteron aisle, improves immunity of organisms, grice diarrhoea rate is declined 54.88%.The fermentation liquid pig starter feed leads to
After everfermentation, the palatability and digestibility of feed are improve, slowed down significantly by breast milk directly to solid feed to weanling pig
Stress, so as to reduce diarrhea rate and the death rate.
More detailed description of the present invention is as follows：
（1）The screening of fermented bacterium
The present invention screens aimed strain from 9 plants of bacterium.Strain category can be divided into 4 plants of brood cell bacterium, be respectively bacillus subtilis
（B10-5）, bacillus subtilis（WL-BA-1）, bacillus amyloliquefaciens（BI2）, bacillus subtilis（BI1）；Lactic acid bacteria 5
Strain, is respectively Lactobacillus plantarum, bacillus coagulans, dregs of beans lactobacillus, enterococcus faecalis LJ, lactobacillus fermenti.
1）The screening of gemma bacteria strain
The brood cell bacterium that will have been activated is put be connected on starch culture-medium and casein culture medium flat plate respectively, is then placed in 37 DEG C of constant temperature
Cultivated in biochemical cultivation case, whether there is transparent circle to produce around observation bacterial strain, picking produces the bacterial strain of transparent circle to activate again, dilute
Release and be applied on flat board, be allowed to produce single bacterium colony, observation produces the size of transparent circle, and the big bacterial strain of picking transparent circle is by producing
The bacterial strain that protease experiment filters out high yield amylase and high proteinase yield from 4 plants of brood cell bacteriums is tested and produced to amylase.Respectively
It is bacillus subtilis B1（CGMCC1.504）, bacillus amyloliquefaciens B2（CGMCC3413）.
2）The screening of lactic acid bacteria strains
The lactic acid bacterial liquid that will have been activated is inoculated in feed according to 5% ratio respectively, is then stirred feed and is allowed to fully mixed
It is even, it is fitted into after fermentation bag is placed in 37 DEG C of environment the 3d that ferments and is detected.5g fermented feeds are weighed during detection to be put into triangular flask,
45ml distilled water vibration 20min is added, is then centrifuged for taking supernatant, with its breast of bio-sensing analysis-e/or determining after the corresponding multiple of dilution
Acid content, chooses lactic acid content 2 plants of bacterial strains high.Screened from 6 strains of lactic acid bacteria by lactic acid bacteria lactic acid producing performance test and produced
Acid lactic acid bacteria high.It is respectively bacillus coagulans R1（CGMCC1.1322）, enterococcus faecalis R2（CGMCC1.130）.
（2）The optimization of fermentation condition：
1）The optimization of fermentation liquid-state feed Bacillus and lactic acid bacteria mixed proportion：
The 2 plants of Bacillus that will be filtered out and 2 strains of lactic acid bacteria activated for 2 generations, and in the fermentation material that access is pre-mixed, inoculum concentration is
8%, Bacillus and lactobacillus inoculum ratio are respectively 1:2,1:1,2:1,3:Isosorbide-5-Nitrae:1,2 plant of Bacillus inoculative proportion is 1:1,2
Strains of lactic acid bacteria inoculative proportion is 1:1, material-water ratio is 1:Fermented in 3.5,37 DEG C of constant temperature biochemical cultivation cases after 3d, detect therein going back
The indexs such as raw sugar content, lactic acid content and prebiotic bacterium number.
Content of reducing sugar, lactic acid content and prebiotic bacterium number after the Bacillus of table 1 and the fermentation of lactic acid bacteria mixed proportion
As can be seen from Table 1, when the mixed proportion of Bacillus and lactic acid bacteria is 2:When 1, content of reducing sugar highest, prebiotic bacterium number
It is higher.Therefore, Bacillus and the mixed proportion selection of lactic acid bacteria are 2:1.
2）2 plants of optimizations of Bacillus ratio of fermented feed：
2 plants of Bacillus and 2 strains of lactic acid bacteria were activated into for 2 generations, in the fermentation material that access is pre-mixed, inoculum concentration is 8%, Bacillus
It is 2 with lactobacillus inoculum ratio:1,2 plant of Bacillus B1 and B2 inoculative proportion is respectively 3:1,2:1,1:1,1:2,1:3,2 plants of breasts
Sour bacterium inoculative proportion is 1:1, material-water ratio is 1:Fermented in 3.5,37 DEG C of constant temperature biochemical cultivation cases after 3d, detect reduced sugar therein
The indexs such as content, lactic acid content and prebiotic bacterium number.
Content of reducing sugar, lactic acid content and prebiotic bacterium number after 2 two kinds of Bacillus different mixing proportion fermentations of table
As can be seen from Table 2, the mixed proportion of Bacillus B1 and B2 is 1:1 compared with other mixed proportions, and content of reducing sugar is most
It is high.Therefore, it is final to determine that 2 plants of mixed proportions of Bacillus B1 and B2 are 1:1.
3）The optimization of the strains of lactic acid bacteria ratio of fermented feed 2:
2 plants of Bacillus and 2 strains of lactic acid bacteria were activated into for 2 generations, in the fermentation material that access is pre-mixed, inoculum concentration is 8%, Bacillus
It is 2 with lactobacillus inoculum ratio:1,2 plant of Bacillus B1 and B2 inoculative proportion is 1:1,2 strains of lactic acid bacteria R1 and R2 ratio is respectively
3:1,2:1,1:1,1:2,1:3, material-water ratio is 1:Fermented in 3.5,37 DEG C of constant temperature biochemical cultivation cases after 3d, detect reduction therein
The indexs such as sugared content, lactic acid content and prebiotic bacterium number.
Content of reducing sugar, lactic acid content and prebiotic bacterium number after 3 two kinds of lactic acid bacteria different mixing proportion fermentations of table
As can be seen from Table 3, two kinds of mixed proportions of lactic acid bacteria R1 and R2 are 1:When 2, content of reducing sugar highest.Therefore, finally
The mixed proportion for determining 2 strains of lactic acid bacteria R1 and R2 is 1:2.
4）The optimization of fermented feed inoculum concentration：
2 plants of Bacillus and 2 strains of lactic acid bacteria were activated into for 2 generations, in the fermentation material that access is pre-mixed, Bacillus and lactobacillus inoculum
Ratio is 2:1,2 plant of Bacillus B1 and B2 inoculative proportion is 1:1,2 strains of lactic acid bacteria R1 and R2 ratio is 1:2, inoculum concentration is respectively
4%, 6%, 8%, 10%, 12%, material-water ratio is 1:Fermented in 3.5,37 DEG C of constant temperature biochemical cultivation cases after 3d, detect reduced sugar therein
The indexs such as content, lactic acid content and prebiotic bacterium number.
Content of reducing sugar, lactic acid content and prebiotic bacterium number after the fermentation of the different vaccination amount of table 4
As can be seen from Table 4, with the increase of inoculum concentration, content of reducing sugar first reduces to increase afterwards and reduces again, is in inoculum concentration
4% with 10% when content of reducing sugar highest and as, lactic acid content is also high, but inoculum concentration high cost also increases, therefore, inoculum concentration is
It is most suitable when 4%.
5）The optimization of fermented feed material-water ratio：
2 plants of Bacillus and 2 strains of lactic acid bacteria were activated into for 2 generations, in the fermentation material that access is pre-mixed, Bacillus and lactobacillus inoculum
Ratio is 2:1,2 plant of Bacillus B1 and B2 inoculative proportion is 1:1,2 strains of lactic acid bacteria R1 and R2 ratio is 1:2, inoculum concentration is 4%, material
Water ratio respectively 1:3,1:3.5,1:4,1:4.5,1:Fermented in 5,37 DEG C of constant temperature biochemical cultivation cases after 3d, detect reduction therein
The indexs such as sugared content, lactic acid content and prebiotic bacterium number.
Content of reducing sugar, lactic acid content and prebiotic bacterium number after the different material-water ratio fermentations of table 5
As can be seen from Table 5, when material-water ratio is 1:When 3.5, content of reducing sugar highest, prebiotic bacterium number is of a relatively high, therefore, selection
Material-water ratio is 1:3.5.
6）The optimization of fermented feed temperature：
2 plants of Bacillus and 2 strains of lactic acid bacteria were activated into for 2 generations, in the fermentation material that access is pre-mixed, Bacillus and lactobacillus inoculum
Ratio is 2:1,2 plant of Bacillus B1 and B2 inoculative proportion is 1:1,2 strains of lactic acid bacteria R1 and R2 ratio is 1:2, inoculum concentration is 4%, material
Water ratio is 1:3.5, be respectively placed in 20 DEG C, 26 DEG C of fermentation 5d, 32 DEG C, 37 DEG C, after 42 DEG C of fermentation 3d, detect that reduced sugar therein contains
The indexs such as amount, lactic acid content and prebiotic bacterium number.
Content of reducing sugar, lactic acid content and prebiotic bacterium number after the different fermentations temperature fermentation of table 6
As seen from Table 6, with the rising of temperature, reduced sugar first increases and reduces afterwards, and 32 DEG C reach highest.During real attenuation
20-30 DEG C of scope may be selected as fermentation temperature.
（3）Optimization of orthogonal test fermentation condition
The 2 plants of Bacillus that will be filtered out and 2 strains of lactic acid bacteria activated for 2 generations, and orthogonal experiment is carried out with the condition that single factor test optimizes, and used
L9(33) to Bacillus and the inoculative proportion of lactic acid bacteria, total inoculum concentration, material-water ratio is divided the experiment of the way crossover of three level of type three
Analysis.After fermentation 3d, its lactic acid content, reduced sugar, gemma bacterium number and lactic acid bacteria number are determined, using content of reducing sugar as index, selected
Content of reducing sugar highest is taken as final feed fermentation condition.
The orthogonal experiment influence factor of table 7 and horizontal list
The range analysis of orthogonal experiment of table 8
The orthogonal test variance analysis of table 9
Knowable to Orthogonal experiment results intuitively analyze R values, the factor of result of the test is influenceed to be followed successively by A>C>B, i.e., to lactic acid producing shadow
The factor of sound is followed successively by inoculum concentration, material-water ratio, inoculative proportion, that is, the condition after optimizing is that inoculum concentration is 4%, Bacillus and lactic acid bacteria
Inoculative proportion is 1:1, material-water ratio is 1:3.5, the 4th group is both this combination in orthogonal test.
(4) preparation method of microbial fermentation child care phase piglet liquid-state feed
Studied by above-mentioned many experiments, the preparation method of final microbial fermentation child care phase piglet liquid-state feed is as follows：
1）By lactic acid bacteria in 42 DEG C of quiescent culture 24h of MRS medium liquids, Bacillus are in 35 DEG C, 200 revs/min of LB culture mediums
Clock, concussion and cultivate 18h obtains lactobacillus solution and Bacillus seed liquor；
LB culture mediums（g/L）：Tryptone 10, yeast extract powder 5, NaCl 10, pH7.5.
MRS culture mediums（g/L）：Peptone 10, beef extract 10, yeast extract powder 5, glucose 5, CH3COONa 5, lemon
Acid diamine 2, Tween 80 1, K2HPO42, MgSO4•7H2O 0.2, MnSO4•7H2O 0. 05, pH6.2.
2）Vegetable raw material is crushed, is well mixed with water, be heated to 100 DEG C, hold time 10min；
3）Treat that temperature drops to 30 DEG C or so, be inoculated with liquid microorganism seed liquid, microorganism seed liquid is mixed by Bacillus, lactic acid bacteria
Bacterium combines.It is 4% ~ 6% that Bacillus amount to inoculum concentration with lactic acid bacteria seed mixture liquid（v/w）, wherein Bacillus and lactic acid bacteria
Inoculative proportion is 2:1~3:1（v/v）, Bacillus B1 is 1 with Bacillus B2 inoculative proportions:1（v/v）, lactic acid bacteria R1 and lactic acid bacteria
R2 inoculative proportions are 1:2（v/v）, material-water ratio is 1:3~1:3.5（w/w）.Load in Plastic Drum or stainless steel cask after well mixed
Fermentation；20-30 DEG C ferments 3-5 days.
The product that microbial fermentation child care phase piglet liquid-state feed disclosed by the invention and preparation method and application has
Pole effect is：
（1）The present invention using Bacillus, lactic acid bacteria and saccharomycete mixed fermentation vegetable raw material, the pig starter feed for thus preparing without
Any antibiotic is added, and rich in nutriments such as lactic acid, protein, small peptide, polysaccharide, vitamin, mineral matter and trace elements, is resisted
Trophic factors is few, with good palatability, it is easy to digest the features such as, probiotics showed increased, and being colonized in enteron aisle can make piglet
Weaning period is smoothly spent, the diarrhea rate of piglet is reduced, the survival rate of piglet is improved.
（2）Pig starter feed of the invention have safe and reliable, drug residue free, good palatability, it is easy to digest the features such as, can be big
Amplitude reduces the use of antibiotic, improves the general level of the health of animal, improves immunity of organisms.The fermentation liquid pig starter feed passes through
After fermentation, the palatability and digestibility of feed are improve, slow down significantly weanling pig is answered to solid feed directly by breast milk
Swash, so that diarrhea rate and the death rate are reduced, additionally, the liquid pig starter feed contains profitable probliotics and its metabolite, with nutrition
With health care double effects.
Brief description of the drawings：
Fig. 1 is the preparation technology flow of microbial fermentation child care phase piglet liquid-state feed.
Specific embodiment
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention
It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention
Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this
On the premise of invention spirit and scope, the various changes that are carried out to the material component and consumption in these embodiments or change
Belong to protection scope of the present invention.Raw materials used and reagent of the invention is commercially available.
Embodiment 1
The composition of liquid bacterium solution：
A kind of liquid bacterium solution for the liquid pig starter feed that ferments, it is combined by Bacillus and lactic acid bacteria；Bacillus with
The volume parts ratio of lactobacillus inoculum is 2:1（v/v）, the Bacillus refer to gemma B1 and Bacillus B2, B1 B2 inoculations to be compared
Example is 1:1（v/v）, the lactic acid bacteria refers to：Lactic acid bacteria R1 and lactic acid bacteria R2, R1 and R2 inoculative proportion are 1:2（v/v）；Its
Middle Bacillus B1 refers to bacillus subtilis（CGMCC 1.504）, Bacillus B2 refers to bacillus amyloliquefaciens
（CGMCC3413）, lactic acid bacteria R1 refers to bacillus coagulans（CGMCC1.1322）, lactic acid bacteria R2 refers to enterococcus faecalis
（CGMCC1.130）.
Embodiment 2
The preparation method of microbial fermentation child care phase piglet liquid-state feed
Studied by above-mentioned many experiments, the preparation method of final microbial fermentation child care phase piglet liquid-state feed is as follows：
1）By lactic acid bacteria in 42 DEG C of quiescent culture 24h of MRS medium liquids, Bacillus are in 35 DEG C, 200 revs/min of LB culture mediums
Clock, concussion and cultivate 18h obtains lactobacillus solution and Bacillus seed liquor；
LB culture mediums（g/L）：Tryptone 10, yeast extract powder 5, NaCl 10, pH7.5.
MRS culture mediums（g/L）：Peptone 10, beef extract 10, yeast extract powder 5, glucose 5, CH3COONa 5, lemon
Acid diamine 2, Tween 80 1, K2HPO42, MgSO4•7H2O 0.2, MnSO4•7H2O 0. 05, pH6.2.
2）Vegetable raw material is crushed, is well mixed with water, be heated to 100 DEG C, hold time 10min；
3）Treat that temperature drops to 30 DEG C or so, be inoculated with liquid microorganism seed liquid, microorganism seed liquid is mixed by Bacillus, lactic acid bacteria
Bacterium combines.It is 4% that Bacillus amount to inoculum concentration with lactic acid bacteria seed mixture liquid（v/w）, wherein Bacillus and lactobacillus inoculum
Ratio is 2:1（v/v）, Bacillus B1 is 1 with Bacillus B2 inoculative proportions:1（v/v）, lactic acid bacteria R1 and lactic acid bacteria R2 inoculations ratio
Example is 1:2（v/v）, material-water ratio is 1:3（w/w）.Load Plastic Drum or stainless steel cask fermentation after well mixed；30 DEG C of fermentations 3
My god.The fermentation child care phase piglet liquid-state feed prepared under this process conditions, content of reducing sugar improves 1.56% before relatively fermenting,
Lactic acid content reaches 4.0%, eliminates pollution of the Escherichia coli to feed.
Embodiment 3
Studied by above-mentioned many experiments, the preparation method of final microbial fermentation child care phase piglet liquid-state feed is as follows：
1）By lactic acid bacteria in 42 DEG C of quiescent culture 24h of MRS medium liquids, Bacillus are in 35 DEG C, 200 revs/min of LB culture mediums
Clock, concussion and cultivate 18h obtains lactobacillus solution and Bacillus seed liquor；
LB culture mediums（g/L）：Tryptone 10, yeast extract powder 5, NaCl 10, pH7.5.
MRS culture mediums（g/L）：Peptone 10, beef extract 10, yeast extract powder 5, glucose 5, CH3COONa 5, lemon
Acid diamine 2, Tween 80 1, K2HPO42, MgSO4•7H2O 0.2, MnSO4•7H2O 0. 05, pH6.2.
2）Vegetable raw material is crushed, is well mixed with water, be heated to 100 DEG C, hold time 10min；
3）Treat that temperature drops to 30 DEG C or so, be inoculated with liquid microorganism seed liquid, microorganism seed liquid is mixed by Bacillus, lactic acid bacteria
Bacterium combines.It is 6% that Bacillus amount to inoculum concentration with lactic acid bacteria seed mixture liquid（v/w）, wherein Bacillus and lactobacillus inoculum
Ratio is 3:1（v/v）, Bacillus B1 is 1 with Bacillus B2 inoculative proportions:1（v/v）, lactic acid bacteria R1 and lactic acid bacteria R2 inoculations ratio
Example is 1:2（v/v）, material-water ratio is 1:3.5（w/w）.Load Plastic Drum or stainless steel cask fermentation after well mixed；20 DEG C of hairs
Ferment 5 days.The fermentation child care phase piglet liquid-state feed prepared under this process conditions, content of reducing sugar improves before relatively fermenting
1.56%, lactic acid content reaches 4.0%, eliminates pollution of the Escherichia coli to feed.
Embodiment 4
The microbial fermentation child care phase piglet liquid-state feed for making in this way, feeds the age in days piglet of 7 age in days -35, totally 24, divides
Two treatment groups, three repetitions of each treatment group, control group fed full price powder, test group microbial fermentation child care phase piglet liquid
State feed substitutes 50% full price powder.
Nursery house is transferred to after experiment weaned piglet immediately, colony house is high bed and puts down foster, is divulged information in holding house smooth.Test group
Use with crib without remaining material as principle, free choice feeding and drinking-water, daily in the morning 08:00th, noon 12:00th, afternoon 17:
00 and evening 21:The 00 time point timing feeding of grade 4, nipple-shaped drinker drinking-water, pig house temperature control at 25-30 DEG C, often
It sweeps 2 pig houses.Other daily managements are routinely carried out.
The full price powder of table 10 and microbial fermentation child care phase piglet liquid-state feed feeding effect
(1) the inherently good feed protein of microbial cells, its protein content is high, and amino acid composition and animal
Required amino acid composition is increasingly similar, it is easier to be digested absorption, therefore feedstuff-meat ratio reduction by 8.65%；
(2) after microbial fermentation, macro-molecular protein is degraded to small-molecule substance, and vegetalitas egg to vegetable protein
The antigenic characteristic reduction of white matter, there is provided bioactivator, such as functional polypeptide, enzyme, improves feed taste, improves
The palatability of feed, daily ingestion amount improves 1.96%.
(3) some ANFs are often contained in plant, such as trypsin ihhibitor and phytate phosphorus, easily
Nutriment is absorbed in influence young animal small intestine, but the big raw material such as corn and dregs of beans by fermentation after, can eliminate
Or above-mentioned ANFs is reduced, and vegetable protein is degraded to small peptide, oligopeptides and free amine group that small intestine is easier to absorb
Acid, microbial fermentation will also produce many enzymes, such as protease, lipase and amylase, can greatly improve the digestion of feed
Absorptivity, daily gain improves 11.68%.
(4) ANFs in feed is reduced due to microbial fermentation, feed digestion absorption efficiency is increased, and
Probiotics in fermented feed improves the micro-ecological environment of enteron aisle, grice diarrhoea rate is declined 54.88%.
Claims (8)
1. a kind of liquid bacterium solution for the liquid pig starter feed that ferments, it is characterised in that it be to be combined by Bacillus and lactic acid bacteria and
Into；Bacillus are 2 with the volume parts ratio of lactobacillus inoculum:1~3:1（v/v）, the Bacillus refer to gemma B1 and gemma
Bacterium B2, B1 B2 inoculative proportions are 1:1（v/v）, the lactic acid bacteria refers to：Lactic acid bacteria R1 and lactic acid bacteria R2, R1 and R2 inoculation ratio
Example is 1:2（v/v）；Wherein Bacillus B1 refers to bacillus subtilis（CGMCC 1.504）, Bacillus B2 refer to solve starch
Bacillus（CGMCC3413）, lactic acid bacteria R1 refers to bacillus coagulans（CGMCC1.1322）, lactic acid bacteria R2 refers to excrement
Enterococcus（CGMCC1.130）.
2. liquid bacterium solution described in a kind of use claim 1 carries out the preparation method of liquid pig starter feed fermentation, it is characterised in that
Comprise the following steps；
1）Piglet full price vegetable raw material is crushed, is well mixed with water, be heated to 100 DEG C, hold time 10min；Its Central Plains
Material is 1 with water ratio:3~1:4.5（w/w）；
2）Treat that temperature drops to 30-35 DEG C, be inoculated with liquid bacterium solution, inoculum concentration is 4% ~ 6%（v/w）, Plastic Drum is loaded after being well mixed
Or stainless steel cask fermentation.
3. the preparation method described in claim 2, it is characterised in that in the step 1）In, complete feed for piglets feed powder particle
Degree 40-60 mesh.
4. the preparation method described in claim 2, it is characterised in that in the step 1）Middle vegetable raw material is matched：Corn
Powder:Dregs of beans=3:1（w/w）.
5. the preparation method described in claim 2, it is characterised in that the step 2）In, 30 DEG C~37 DEG C of fermentation temperature, fermentation
Time is 4 days~10 days, obtains the liquid pig starter feed that ferments；Liquid-state feed content of reducing sugar reaches more than 1.56%, lactic acid content
More than 4.0% is reached, lactic acid bacteria number reaches 109More than cfu/g, E. coli detection less than.
6. the utilization rate of feed is being improved for liquid pig starter feed liquid bacterium solution of fermenting described in claim 1, improving feed
Application in terms of local flavor.
7. described in claim 1 for ferment liquid pig starter feed liquid bacterium solution improve preventing and treating weanling pig syndrome in terms of
Application.
8. the application described in claim 7, wherein the diarrhea rate that preventing and treating weanling pig syndrome refers to reducing piglet reaches 50%
More than.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611114578.5A CN106721278A (en) | 2016-12-07 | 2016-12-07 | Microbial fermentation child care phase piglet liquid-state feed and preparation method and application |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109043122A (en) * | 2018-09-29 | 2018-12-21 | 天津科技大学 | For the liquid bacterium solution of fermentation cassava dreg fodder and its preparation method of Starch Tapioca residue fermented feed |
| CN111466481A (en) * | 2020-04-13 | 2020-07-31 | 博益德（北京）生物科技有限公司 | Liquid bacterium solution for fermenting liquid piglet feed, preparation method and application of liquid fermented piglet feed |
| CN112375701A (en) * | 2020-11-11 | 2021-02-19 | 重庆美邦农生物技术有限公司 | Microbial preparation for improving intestinal environment of ruminant such as cattle and sheep and preparation method thereof |
| CN115322925A (en) * | 2022-07-18 | 2022-11-11 | 山西农业大学 | Liquid state fermentation complete feed for pigs and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101341927A (en) * | 2008-08-29 | 2009-01-14 | 罗志诣 | Microbial feed additive liquid agent for pig and preparation technique |
| CN103070285A (en) * | 2013-01-10 | 2013-05-01 | 北京农学院 | Microbe feed additive and preparation method thereof |
| CN103907749A (en) * | 2014-04-10 | 2014-07-09 | 李德元 | Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof |
| CN105410410A (en) * | 2015-12-29 | 2016-03-23 | 雏鹰农牧集团股份有限公司 | Pig complete fermented feed and preparation method thereof |
-
2016
- 2016-12-07 CN CN201611114578.5A patent/CN106721278A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101341927A (en) * | 2008-08-29 | 2009-01-14 | 罗志诣 | Microbial feed additive liquid agent for pig and preparation technique |
| CN103070285A (en) * | 2013-01-10 | 2013-05-01 | 北京农学院 | Microbe feed additive and preparation method thereof |
| CN103907749A (en) * | 2014-04-10 | 2014-07-09 | 李德元 | Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof |
| CN105410410A (en) * | 2015-12-29 | 2016-03-23 | 雏鹰农牧集团股份有限公司 | Pig complete fermented feed and preparation method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109043122A (en) * | 2018-09-29 | 2018-12-21 | 天津科技大学 | For the liquid bacterium solution of fermentation cassava dreg fodder and its preparation method of Starch Tapioca residue fermented feed |
| CN111466481A (en) * | 2020-04-13 | 2020-07-31 | 博益德（北京）生物科技有限公司 | Liquid bacterium solution for fermenting liquid piglet feed, preparation method and application of liquid fermented piglet feed |
| CN112375701A (en) * | 2020-11-11 | 2021-02-19 | 重庆美邦农生物技术有限公司 | Microbial preparation for improving intestinal environment of ruminant such as cattle and sheep and preparation method thereof |
| CN115322925A (en) * | 2022-07-18 | 2022-11-11 | 山西农业大学 | Liquid state fermentation complete feed for pigs and preparation method and application thereof |
| CN115322925B (en) * | 2022-07-18 | 2023-08-15 | 山西农业大学 | Liquid fermented complete feed for pigs and preparation method and application thereof |
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