CN108402320A - A kind of composite probiotics preparations and its application in pig starter feed - Google Patents
A kind of composite probiotics preparations and its application in pig starter feed Download PDFInfo
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- CN108402320A CN108402320A CN201810186361.8A CN201810186361A CN108402320A CN 108402320 A CN108402320 A CN 108402320A CN 201810186361 A CN201810186361 A CN 201810186361A CN 108402320 A CN108402320 A CN 108402320A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 139
- 239000006041 probiotic Substances 0.000 title claims abstract description 94
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 94
- 239000002131 composite material Substances 0.000 title claims abstract description 70
- 239000007858 starting material Substances 0.000 title claims description 15
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 46
- 241001465754 Metazoa Species 0.000 claims abstract description 46
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 45
- 241000194020 Streptococcus thermophilus Species 0.000 claims abstract description 42
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 41
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims abstract description 40
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims abstract description 40
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 39
- CANRESZKMUPMAE-UHFFFAOYSA-L Zinc lactate Chemical compound [Zn+2].CC(O)C([O-])=O.CC(O)C([O-])=O CANRESZKMUPMAE-UHFFFAOYSA-L 0.000 claims abstract description 39
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 38
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 33
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 33
- 229940050168 zinc lactate Drugs 0.000 claims abstract description 33
- 239000011576 zinc lactate Substances 0.000 claims abstract description 33
- 235000000193 zinc lactate Nutrition 0.000 claims abstract description 33
- 229920000926 Galactomannan Polymers 0.000 claims abstract description 23
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 claims abstract description 18
- 239000000843 powder Substances 0.000 claims description 59
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- 229940039696 lactobacillus Drugs 0.000 claims 1
- 125000000430 tryptophan group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C2=C([H])C([H])=C([H])C([H])=C12 0.000 claims 1
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- 206010012735 Diarrhoea Diseases 0.000 abstract description 29
- 102000004889 Interleukin-6 Human genes 0.000 abstract description 9
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- LOGFVTREOLYCPF-KXNHARMFSA-N (2s,3r)-2-[[(2r)-1-[(2s)-2,6-diaminohexanoyl]pyrrolidine-2-carbonyl]amino]-3-hydroxybutanoic acid Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@H]1CCCN1C(=O)[C@@H](N)CCCCN LOGFVTREOLYCPF-KXNHARMFSA-N 0.000 abstract 1
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- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 4
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- 241000194032 Enterococcus faecalis Species 0.000 description 4
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- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
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- 235000008935 nutritious Nutrition 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/515—Animalis
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Birds (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Inorganic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
Abstract
A kind of composite probiotics preparations, which is characterized in that the composite probiotics preparations are made of bacillus licheniformis, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus, streptococcus thermophilus, tryptophan, xylo-oligosaccharide, galactomannans and zinc lactate.The composite probiotics preparations of the present invention can effectively improve the growing state of piglet, it improves Average weight increasing a day, reduce diarrhea rate, interleukin-1 beta (1 β of IL), interleukin-6 (IL 6) and tumor necrosis factor α (TNF α), the intestinal mucosa SIgA that can also be improved simultaneously in piglet body are horizontal, improve the disease resistance of piglet, moreover it is possible to piglet be helped to establish the intestinal flora environment of health.And production cost is low, utilization easy to spread, has wide utilization foreground.
Description
Technical field:
The invention belongs to biotechnologies and field of fodder, and in particular to a kind of composite probiotics preparations and its in piglet
Application in feed.
Background technology:
During modern large scale of pig farm, weaned piglet is an important link in growth course, due to early weaning,
What weanling piglet faced first is exactly the change of environment and feed, inevitably results from a series of psychology, environment, battalion
The stress reaction of support etc., and intestine of young pigs immune system could generally be reached maturity in 4~7 week old substantially, therefore it is early
Piglet itself generates immune antiboidy level when phase weans and cell immunocompetent is relatively low, along with having interrupted maternal immunity antibody
It providing, the immunity function for keeping piglet not perfect is further weakened by that stress be influenced, and piglet body circulation antibody level reduces,
Inhibit cell immunocompetent, show significant immunosupress, so as to cause intestine of young pigs flora imbalance, feed intake declines, disappears
Change bad, disease-resistant force difference, slow-growing, diarrhea is even dead, these problems annoying the development of intensive industrialized piggery always.Base
In the presence of problem above, antibiotic largely uses in weanling pig daily ration, as the drug resistance that abuse of antibiotics is brought is asked
Topic and problem of environmental pollution are paid more and more attention, and feeding antibiotic, which is used for a long time, can make pathogenic bacteria generate drug resistance and pass through food
Chain threatens human health and safety belt, therefore it is already that aquaculture is badly in need of solving to find environmentally protective Substitutes For Antibiotic
One of the problem of.
Probiotics is also known as raw element of probiotic, active bacteria formulation, profit etc., is proposed by Parker, it is a kind of dynamic by adjusting
Object gastrointestinol microorganism colony balance is to realize the micro- life of activity for preventing disease, improving feed digestibility and promoting growth of animal
The general designation of object, culture and other products.Probiotics is flat by adjusting Tiny ecosystem as a kind of new green environment protection additive
Weighing apparatus and desirable influence is generated to animal, can Direct-fed or with Basic drawing mixed feeding, no drug resistance, noresidue, without pair
It acts on, is safe and reliable, free from environmental pollution, developing and utilize and paid attention to by various countries.Probiotic products are rationally utilized, can be improved
The growth performance and immune level of animal, can effectively prevent and treat grice diarrhoea, reduce stress generation, be conducive to son
The growth of pig.Probiotics effectively can adjust and maintain the colony balance of enteron aisle, improve livestock and poultry cultivation environment, improve feed conversion
Rate improves the barrier function of animal and bird intestines, enhances the immunity of body, is generally acknowledged one of optimum antibiotic substitute.
In recent years about the addition of probiotics in feed to weanling pig growth and development, intestinal flora, immune function and
The research of diarrhea situation etc. is more.The study found that probiotics is applied in pig production, weaning pigs weight can be significantly reduced
Than reducing Stomach duodenum pH value, improving liver index, adjust weanling pig intestinal health.Occupation rate is higher on Vehicles Collected from Market
Probiotic products are mainly bacillus subtilis mushroom, lactic acid bacteria class and budding fungus.It is reported that lactic acid bacteria can improve chitling road
Tiny ecosystem and health status improve the growth performance of suckling pig;Yeast Propagation increases into the mechanism that fiber feedstuff is degraded with it
Rumen Fungi quantity is related with cellulase activity;After bacillus subtilis enters animal intestinal tract, enteron aisle aerogenesis pod can significantly reduce
Film clostridium, escherichia coli and detection of Salmonella quantity, and to the development of intestinal villi have significant facilitation.
CN201310633814.4 provides a kind of piglet composite probiotics preparations product and method, should in terms of mass parts
Composite probiotics preparations contain 40-80 parts of yeast, 80-110 parts of enterococcus faecalis, 400-520 parts of bacillus subtilis, zytase
150-180 parts, 5-12 parts of mannase, 30-70 parts of cellulase, 0-120 parts of 10-40 parts of protease and amylase 8;More
Preferably, said preparation includes 66 parts of yeast, 98 parts of enterococcus faecalis, 492 parts of bacillus subtilis, 165 parts of zytase, sweet dew
96 parts of 9 parts of dextranase, 49 parts of cellulase, 25 parts of protease and amylase.The composite probiotics preparations are added to child care pig
Piglet is fed in daily ration, can enhance piglet immunity of organisms, reduces diarrhea, promotes piglet growth, improves piglet weightening.
CN201210391466.X provides boar composite probiotics preparations and preparation method thereof, component and weight
Part is as follows:Lactobacillus rhamnosus preparation 20-30, enterococcus faecalis preparation 15-25, lactic acid bacillus preparation 8-18, wax-like gemma
Bacillus preparation 7-17, bacillus subtilis formulation 10-20, brewer's yeast preparation 10-20;Each component prepares bacterial strain uses therefor
Lactobacillus rhamnosus LT22, enterococcus faecalis CH13, bacillus SC27, Bacillus cercus SL1, bacillus subtilis and beer
Yeast;The techniques such as the activated culture of each component, expansion culture, freeze-drying and wheat bran absorption are made, and mix in proportion i.e.
Pig composite probiotics preparations are obtained, liquid dosage form and solid dosage forms are divided into.Said preparation contains more probiotics of high activity, thalline
Cell resistance power is strong, metabolite is abundant, nutritious to pig, immune and prebiotic effect, especially to pregnant sow and weanling pig
Using effect is good.
CN201310739737.0 discloses a kind of suckling piglet specific enzyme and preparation method thereof containing profitable probliotics, described to contain
The suckling piglet specific enzyme of profitable probliotics is grouped as by the group of following parts by weight:5-20 parts of Chinese herbal medicine powder, zytase 800-
1200 parts, 80-120 parts of cellulase, 250-300 parts, mannase 15-25 of bacillus licheniformis culture, protease
600-1000,50-80 parts of mesophilicα-diastase.Preparation method is as follows:After bacillus licheniformis is fermented by filtering, concentration,
The dry culture obtained is compounded with other compositions, and gained complex enzyme can improve food conversion ratio, and reduces feed cost, is improved and is protected
Deposit stability.
However, either the mixed fermentation product of single culture tunning or several probiotics, either preparation are straight
It connects and gavages or mixed with its Basic drawing, performance results are also unstable, this mainly (adds with the type of probiotics, viable bacteria concentration
Add ratio), to feed mode related with pig age in days.
Invention content:
The problem of developing for current composite probiotics preparations cultivates in conjunction with pig, is especially deposited in piglet cultivation
Weaning stress caused by intestinal bacilli illness, feed intake decline, indigestion, disease-resistant force difference is slow-growing, and diarrhea is even
The technical problems such as death, the present invention is intended to provide a kind of composite probiotics preparations and its application in pig starter feed.
In order to solve the above technical problems, present invention employs following technological means:
A kind of composite probiotics preparations, which is characterized in that the composite probiotics preparations are by bacillus licheniformis, wine brewing ferment
Mother, animal bifidobacteria, lactobacillus acidophilus, streptococcus thermophilus, tryptophan, xylo-oligosaccharide, galactomannans and zinc lactate group
At.
Preferably, the composite probiotics preparations by following weight point at being grouped as:10-15 parts of bacillus licheniformis,
2-4 parts of saccharomyces cerevisiaes, 20-30 parts of animal bifidobacterias, 20-30 parts of lactobacillus acidophilus, 3-5 parts of streptococcus thermophilus, 0.0004-
0.0006 part of tryptophan, 20-30 parts of xylo-oligosaccharides, 2-3 portions of galactomannans and 0.00003-0.00008 parts of zinc lactate groups
At.
Preferably, the composite probiotics preparations by following weight point at being grouped as:12 parts of bacillus licheniformis, 3 parts
Saccharomyces cerevisiae, 25 parts of animal bifidobacterias, 25 parts of lactobacillus acidophilus, 4 parts of streptococcus thermophilus, 0.0005 part of tryptophan, 25 parts it is low
Xylan, 2.5 portions of galactomannans and 0.00005 part of zinc lactate composition.
Preferably, the composite probiotics preparations by following weight point at being grouped as:10 parts of bacillus licheniformis, 2 parts
Saccharomyces cerevisiae, 30 parts of animal bifidobacterias, 20 parts of lactobacillus acidophilus, 3 parts of streptococcus thermophilus, 0.0004 part of tryptophan, 20 parts it is low
Xylan, 2 portions of galactomannans and 0.00008 part of zinc lactate composition.
Preferably, the composite probiotics preparations by following weight point at being grouped as:15 parts of bacillus licheniformis, 3 parts
Saccharomyces cerevisiae, 20 parts of animal bifidobacterias, 30 parts of lactobacillus acidophilus, 5 parts of streptococcus thermophilus, 0.0006 part of tryptophan, 30 parts it is low
Xylan, 3 portions of galactomannans and 0.00003 part of zinc lactate composition.
Preferably, wherein bacillus licheniformis, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus, streptococcus thermophilus are equal
For bacterium powder dosage form, effectively viable bacteria content is 1.0 × 10 in Bacillus licheniformis powder preparation8CFU/g-2.0×108CFU/
G, effectively viable bacteria content is 1.5 × 10 in S. cervisiae powder preparation8CFU/g-2.0×108CFU/g, animal bifidobacteria bacterium powder
Effective viable bacteria content is 1.0 × 10 in preparation9CFU/g-2.0×109CFU/g, effectively viable bacteria contains in lactobacillus acidophilus bacterium powder preparation
Amount is 5.0 × 108CFU/g-8.0×108CFU/g, effectively viable bacteria content is 3.0 × 10 in streptococcus thermophilus bacterium powder preparation7CFU/
g-5.0×107CFU/g。
Preferably, wherein bacillus licheniformis, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus, streptococcus thermophilus are equal
For bacterium powder dosage form, effectively viable bacteria content is 1.5 × 10 in Bacillus licheniformis powder preparation8CFU/g, saccharomyces cerevisiae bacterium powder
Effective viable bacteria content is 1.75 × 10 in preparation8CFU/g, in animal bifidobacteria bacterium powder preparation effectively viable bacteria content be 1.5 ×
109CFU/g, effectively viable bacteria content is 6.5 × 10 in lactobacillus acidophilus bacterium powder preparation8CFU/g, in streptococcus thermophilus bacterium powder preparation
Effective viable bacteria content is 4.0 × 107CFU/g。
The preparation method of the composite probiotics preparations is also claimed in the present invention, which is characterized in that the preparation method
Include the following steps:Step 1 weighs bacillus licheniformis, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus by weight
With streptococcus thermophilus bacterium powder, tryptophan, xylo-oligosaccharide, galactomannans and zinc lactate;Step 2, the lichens bud that will be weighed
Spore bacillus, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus and streptococcus thermophilus bacterium powder, tryptophan, xylo-oligosaccharide, gala
Mannosan and zinc lactate are uniformly mixed, and are then dispensed, are placed at 4 DEG C and preserve.
Application of the composite probiotics preparations in pig starter feed is also claimed in the present invention, which is characterized in that described
Additive amount of the composite probiotics preparations in pig starter feed is 0.2-0.4% (mass percent).
A kind of pig starter feed is also claimed in the present invention, which is characterized in that 0.2-0.4% is added in the pig starter feed
The composite probiotics preparations of mass percentage.
A kind of method for breeding of piglet is also claimed in the present invention, which is characterized in that using added with 0.2-0.4% mass
The pig starter feed of the composite probiotics preparations of percentage composition is fed.
Based on above technical scheme, the invention has the advantages that and advantageous effect:
First, immunity of organisms is low, intestines according to the physiology and growth characteristic of piglet, especially after weaning stress by the present invention
The characteristics of road normal flora is not yet established uses bacillus licheniformis, saccharomyces cerevisiae, moves in conjunction with the nutritional need of probiotics
It is prepared by object Bifidobacterium, lactobacillus acidophilus, streptococcus thermophilus, tryptophan, xylo-oligosaccharide, galactomannans and zinc lactate combination
The composite probiotics preparations of the present invention are obtained, which can effectively improve the growing state of piglet, carry
High Average weight increasing a day reduces diarrhea rate, while can also improve the interleukin-1 ' beta ' in piglet body (IL-1 β), interleukin-6
(IL-6) and tumor necrosis factor-alpha (TNF-α), intestinal mucosa SIgA are horizontal, improve the disease resistance of piglet, moreover it is possible to help young
Pig establishes the intestinal flora environment of health.
Second, by experiments have shown that, the present invention it is Promethean use tryptophan, xylo-oligosaccharide, galactomannans and
The mode of zinc lactate combination can effectively improve the using effect of probiotics preparation, and experiment comparison result is it is found that simple addition
Tryptophan or zinc lactate cannot obtain good technique effect, only when the two is added simultaneously in use, could obtain good
Technique effect, diarrhea rate falls to the 1/5 of control group 1-3, and Average weight increasing a day improves 10g/d or more, illustrate tryptophan and
Zinc lactate has the function of synergy in composite probiotics preparations.The ratio and tryptophan of oligosaccharide, zinc lactate add
Add and has great importance for the present invention, the restriction of exactly this oligosaccharide adding proportion and tryptophan, zinc lactate
Addition achieves unexpected technique effect to the raising of piglet immunological ability.The ratio and tryptophan of oligosaccharide, lactic acid
The addition of zinc has great importance for the present invention, the restriction of exactly this oligosaccharide adding proportion and tryptophan, breast
The addition of sour zinc achieves unexpected technique effect to the raising of piglet immunological ability.
In conclusion the composite probiotics preparations of the present invention can effectively improve the growing state of piglet, improve average daily
Weightening reduces diarrhea rate, while can also improve the interleukin-1 ' beta ' in piglet body (IL-1 β), interleukin-6 (IL-6)
It is horizontal with tumor necrosis factor-alpha (TNF-α), intestinal mucosa SIgA, improve the disease resistance of piglet, moreover it is possible to piglet be helped to establish
The intestinal flora environment of health.And production cost is low, utilization easy to spread, has wide utilization foreground.
Specific implementation mode:
Embodiment 1:A kind of composite probiotics preparations, the composite probiotics preparations are by following weight point at being grouped as:
12 parts of bacillus licheniformis, 3 parts of saccharomyces cerevisiaes, 25 parts of animal bifidobacterias, 25 parts of lactobacillus acidophilus, 4 parts of streptococcus thermophilus,
0.0005 part of tryptophan, 25 parts of xylo-oligosaccharides, 2.5 portions of galactomannans and 0.00005 part of zinc lactate composition.
Wherein bacillus licheniformis, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus, streptococcus thermophilus are bacterium powder
Dosage form, effectively viable bacteria content is 1.5 × 10 in Bacillus licheniformis powder preparation8CFU/g, in S. cervisiae powder preparation
Effective viable bacteria content is 1.75 × 108CFU/g, effectively viable bacteria content is 1.5 × 10 in animal bifidobacteria bacterium powder preparation9CFU/
G, effectively viable bacteria content is 6.5 × 10 in lactobacillus acidophilus bacterium powder preparation8CFU/g is effectively living in streptococcus thermophilus bacterium powder preparation
Bacterial content is 4.0 × 107CFU/g。
The preparation method of the composite probiotics preparations includes the following steps:Step 1 weighs lichens gemma by weight
Bacillus, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus and streptococcus thermophilus bacterium powder, tryptophan, xylo-oligosaccharide, gala are sweet
Reveal glycan and zinc lactate;Step 2, by the bacillus licheniformis weighed, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus
With streptococcus thermophilus bacterium powder, tryptophan, xylo-oligosaccharide, galactomannans and zinc lactate be uniformly mixed, then packing,
It is placed at 4 DEG C and preserves.
Embodiment 2:A kind of composite probiotics preparations, the composite probiotics preparations are by following weight point at being grouped as:
10 parts of bacillus licheniformis, 2 parts of saccharomyces cerevisiaes, 30 parts of animal bifidobacterias, 20 parts of lactobacillus acidophilus, 3 parts of streptococcus thermophilus,
0.0004 part of tryptophan, 20 parts of xylo-oligosaccharides, 2 portions of galactomannans and 0.00008 part of zinc lactate composition.
Wherein bacillus licheniformis, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus, streptococcus thermophilus are bacterium powder
Dosage form, effectively viable bacteria content is 2.0 × 10 in Bacillus licheniformis powder preparation8CFU/g, in S. cervisiae powder preparation
Effective viable bacteria content is 2.0 × 108CFU/g, effectively viable bacteria content is 2.0 × 10 in animal bifidobacteria bacterium powder preparation9CFU/g,
Effective viable bacteria content is 8.0 × 10 in lactobacillus acidophilus bacterium powder preparation8CFU/g, effective viable bacteria in streptococcus thermophilus bacterium powder preparation
Content is 5.0 × 107CFU/g。
The preparation method of the composite probiotics preparations includes the following steps:Step 1 weighs lichens gemma by weight
Bacillus, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus and streptococcus thermophilus bacterium powder, tryptophan, xylo-oligosaccharide, gala are sweet
Reveal glycan and zinc lactate;Step 2, by the bacillus licheniformis weighed, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus
With streptococcus thermophilus bacterium powder, tryptophan, xylo-oligosaccharide, galactomannans and zinc lactate be uniformly mixed, then packing,
It is placed at 4 DEG C and preserves.
Embodiment 3:A kind of composite probiotics preparations, the composite probiotics preparations are by following weight point at being grouped as:
15 parts of bacillus licheniformis, 3 parts of saccharomyces cerevisiaes, 20 parts of animal bifidobacterias, 30 parts of lactobacillus acidophilus, 5 parts of streptococcus thermophilus,
0.0006 part of tryptophan, 30 parts of xylo-oligosaccharides, 3 portions of galactomannans and 0.00003 part of zinc lactate composition.
Wherein bacillus licheniformis, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus, streptococcus thermophilus are bacterium powder
Dosage form, effectively viable bacteria content is 1.0 × 10 in Bacillus licheniformis powder preparation8CFU/g, in S. cervisiae powder preparation
Effective viable bacteria content is 1.5 × 108CFU/g, effectively viable bacteria content is 1.0 × 10 in animal bifidobacteria bacterium powder preparation9CFU/g,
Effective viable bacteria content is 5.0 × 10 in lactobacillus acidophilus bacterium powder preparation8CFU/g, effective viable bacteria in streptococcus thermophilus bacterium powder preparation
Content is 3.0 × 107CFU/g。
The preparation method of the composite probiotics preparations includes the following steps:Step 1 weighs lichens gemma by weight
Bacillus, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus and streptococcus thermophilus bacterium powder, tryptophan, xylo-oligosaccharide, gala are sweet
Reveal glycan and zinc lactate;Step 2, by the bacillus licheniformis weighed, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus
With streptococcus thermophilus bacterium powder, tryptophan, xylo-oligosaccharide, galactomannans and zinc lactate be uniformly mixed, then packing,
It is placed at 4 DEG C and preserves.
Embodiment 4:Safety testing
(1) acute toxicity test in mice:Mix is carried out using the composite probiotics preparations and feed of the embodiment of the present invention 1
(mass ratio 0.08:1, i.e., in the ratio mix of 0.08kg composite probiotics preparations and 1kg feeds) mouse is fed afterwards, it is continuous to see
It examines 30 days, dead and undue toxicity reaction, off-test, anatomic observation liver,kidney,spleen, stomach, the heart, lung and intestines is had no during experiment
Equal tissues are showed no abnormal change.According to acute toxicity criteria, which belongs to nontoxic grade.
(2) chronic toxicity test:Mix (quality is carried out using the composite probiotics preparations and feed of the embodiment of the present invention 1
Than being 0.02:1, i.e., in the ratio mix of 0.02kg composite probiotics preparations and 1kg feeds) mouse is fed afterwards, continuously feed 80
It, as a result shows:The mouse fur of feeding composite probiotics preparations of the present invention is smooth, and drinking-water of ingesting is normal, and behavioral activity is normal,
Blood parameters and hematology fluctuate in normal range (NR), organize internal organs no abnormality seen, generally speaking have no that toxicity reacts.
(3) Piglet feed test:Use composite probiotics preparations and the feed of the present invention 1 carry out mix (mass ratio for
0.3:100, i.e., in the ratio mix of 0.3kg composite probiotics preparations and 100kg feeds) piglet is fed afterwards, continuous feeding 40 days,
As a result it shows:Piglet well-grown, the hair color of feeding composite probiotics preparations of the present invention are smooth, Gain weight is substantially better than does not make
With the piglet of composite probiotics preparations of the present invention, the symptoms such as rare diarrhea of piglet, it is dead to there is not piglet during entire feeding
Generally speaking the phenomenon that dying has no any toxicity reaction.
Embodiment 5:The influence of oligosaccharide, tryptophan and zinc lactate for composite probiotics preparations using effect
In R&D process of the present invention, inventor is special according to the growth characteristic of microorganism and the nutrition of piglet and growth
Point has finally determined technical scheme of the present invention through repetition test, and in comparison, which there is best technology to imitate
Fruit, can greatly promote and piglet in beneficial bacteria of intestinal tract flora growth and colony balance, improve the immunocompetence of piglet, reduce
The generation of diarrhea, in order to embody relevant technique effect, the following selected part test recipe of inventor is proved and is illustrated, tool
Body formula is referring to the following table 1:
1 embodiment 1 of table and comparative examples
Embodiment 1 | Control 1 | Control 2 | Control 3 | Control 4 | Control 5 | |
Bacillus licheniformis | 12 | 12 | 12 | 12 | 12 | 12 |
Saccharomyces cerevisiae | 3 | 3 | 3 | 3 | 3 | 3 |
Animal bifidobacteria | 25 | 25 | 25 | 25 | 25 | 25 |
Lactobacillus acidophilus | 25 | 25 | 25 | 25 | 25 | 25 |
Streptococcus thermophilus | 4 | 4 | 4 | 4 | 4 | 4 |
Tryptophan | 0.0005 | 0 | 0.0005 | 0 | 0.0005 | 0.0005 |
Xylo-oligosaccharide | 25 | 25 | 25 | 25 | 27.5 | 13 |
Galactomannans | 2.5 | 2.5 | 2.5 | 2.5 | 0 | 13 |
Zinc lactate | 0.00005 | 0.00005 | 0 | 0 | 0.00005 | 0.00005 |
Wherein bacillus licheniformis, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus, streptococcus thermophilus are bacterium powder
Dosage form, effectively viable bacteria content is 1.5 × 10 in Bacillus licheniformis powder preparation8CFU/g, in S. cervisiae powder preparation
Effective viable bacteria content is 1.75 × 108CFU/g, effectively viable bacteria content is 1.5 × 10 in animal bifidobacteria bacterium powder preparation9CFU/
G, effectively viable bacteria content is 6.5 × 10 in lactobacillus acidophilus bacterium powder preparation8CFU/g is effectively living in streptococcus thermophilus bacterium powder preparation
Bacterial content is 4.0 × 107CFU/g。
Screened 140 suckling pigs are equally divided into blank control group, 1 test group of embodiment, comparative examples examination
Group is tested, every group 20, every group of 4 repetitions each repeat 5, record of weighing per the disconnected navel of column piglet childbirth end and wean, experiment
Number of days is 30d.The corresponding probiotics preparation of 0.3% mass percent is added in pig starter feed used by test group, it is right
It is fed using conventional pig starter feed according to group, the equal Free nursing of piglet and feeding.
(1) influence of the probiotics preparation to piglet average daily gain and diarrhea rate
Weigh in the morning on an empty stomach when piglet just birth and wean, calculates average daily gain and the diarrhea rate of every group of piglet.Wherein
Diarrhea rate calculates:The health status of every group of swinery is observed daily, records diarrhea situation, grice diarrhoea is finally counted as unit of group
Head number (1 grice diarrhoea, 1 day note, 1 diarrhea.
Every group of diarrhea total degree/(the total head number × raising number of days of every group of pig in experimental period) of diarrhea rate (%)=in experimental period.
Influence of 2 probiotics preparation of table to piglet average daily gain and diarrhea rate
By with the test result of upper table 2 it is found that the embodiment of the present invention 1 composite probiotics preparations feed piglet, average daily
It is better than control group and blank control group, for Average weight increasing a day rate compares best control group 5, daily body weight gain in terms of weightening
Nearly 8g/d is also improved, and diarrhea rate also just corresponds to the 1/4 of control group 5, the two is compared, two kinds only in composite probiotics preparations
The adding proportion of oligosaccharide is different, this has also confirmed the conclusion (of pressure testing) of inventor, when the ratio of xylo-oligosaccharide and galactomannans
When example is 10 or so, and when xylo-oligosaccharide occupies leading position, best test effect could be obtained, and only with xylo-oligosaccharide
Or both ratio it is close when, effect is worse than the embodiment of the present invention 1.Pass through the comparison sheet of embodiment 1 and control group 1-3
It is bright, whether distinguishing the addition for essentially consisting in tryptophan and zinc lactate therebetween, based on the above experiment comparison result it is found that simple add
Tryptophan or zinc lactate cannot obtain good technique effect, only when the two is added simultaneously in use, could obtain good
Technique effect, diarrhea rate falls to the 1/5 of control group 1-3, and Average weight increasing a day improves 10g/d or more, illustrate tryptophan and
Zinc lactate has the function of synergy in composite probiotics preparations.
(2) influence of the probiotics preparation to piglet serum cytokines, intestinal mucosa SIgA
In experiment 21 days, each group detected interleukin-1 ' beta ' in 20% ratio, 4 parts of blood sampling at random using ELISA method
(IL-1 β), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α).In experiment the 30th day, 1 experiment of every group of Tu
With pig, takes about 10 centimetres of duodenums to cut, fully rinse tissue mucosa mucus with physiological saline, detected using ELISA method
Intestinal mucosa SIgA is horizontal.Specific test result is shown in Table 3:
Influence of 3 probiotics preparation of table to piglet serum cytokines, intestinal mucosa SIgA
By the above test result it is found that the use of probiotics preparation can effectively improve the immune level of piglet, and lead to
The comparison crossed between embodiment 1 and control group 1-5 shows that the composite probiotics preparations of embodiment 1 compare control group more can be effective
Raising piglet disease resistance, achieve unexpected technique effect.Embodiment 1 is white thin compared to the control group for 1-5
- 1 β of born of the same parents' interleukin (IL-1 β), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) and intestinal mucosa SIgA water
Averagely it is higher than control group, the addition of the ratio and tryptophan, zinc lactate that illustrate oligosaccharide is for the present invention with important
Meaning, the raising of the restriction of exactly this oligosaccharide adding proportion and the addition of tryptophan, zinc lactate to piglet immunological ability
Achieve unexpected technique effect.
Because of piglet digestive system, immunity function hypoplasia, it is easy to induce the symptoms such as piglet indigestion, diarrhea, currently
The morbidity and mortality of grice diarrhoea are all very high, and the composite probiotics preparations of the present invention can significantly improve the immune of piglet
Ability, improves its immune level and disease resistance, effectively avoids the generation of diarrhea, from the result of table 1 it can also be seen that this hair
Bright composite probiotics preparations significantly reduce the Incidence of Diarrhea of piglet by the immune disease-resistance ability of improvement piglet.
(3) influence of the probiotics preparation to piglet fecal microorganism
When testing the 21st day, every group each repeats to choose 1 piglet close to average weight, acquires fresh excreta sample
Product are placed in -18 DEG C of refrigerators and preserve, detection Bacillus acidi lactici, Bifidobacterium and Escherichia coli quantity.Microflora is with every gram of excrement
Logarithm (the log of contained bacterial community sum10CFU/g it) indicates.1g excrement is weighed in aseptic operating platform carries glass in 99ml
It is shaken up in the sterile saline of pearl, takes 1mL in test tube, sterile 0.9% physiological saline of 9mL is added, vibrated mixing, press successively
10 times of proportional diluteds are to 10-7.Escherichia coli culture medium is LB culture mediums, and Bacillus acidi lactici culture medium is MS culture mediums, Bifidobacterium
Culture medium is LB agar mediums, carries out coated plate and culture.
Influence of 4 probiotics preparation of table to piglet fecal microorganism
Blank control | Embodiment 1 | Control 1 | Control 2 | Control 3 | Control 4 | Control 5 | |
Bifidobacterium | 7.78 | 8.79 | 8.45 | 8.12 | 7.89 | 8.02 | 8.37 |
Bacillus acidi lactici | 8.55 | 9.24 | 9.01 | 8.87 | 8.66 | 8.89 | 9.05 |
Escherichia coli | 8.98 | 8.06 | 8.35 | 8.56 | 8.68 | 8.43 | 8.35 |
By the above test result it is found that the use of probiotics preparation can effectively improve intestine of young pigs flora, Neng Gouzeng
Add the content of the probiotics such as anaerobic bacteria Bifidobacterium and Bacillus acidi lactici, while the increasing of Escherichia coli can also be reduced to a certain extent
It grows, to play the role of adjusting intestinal flora.Embodiment 1 for 1-5, promotes Bifidobacterium and lactic acid bar compared to the control group
The effect of bacterium and inhibition Escherichia coli are higher than control group, illustrate the ratio of oligosaccharide and the addition pair of tryptophan, zinc lactate
Have great importance for the present invention, the addition of the restriction and tryptophan, zinc lactate of exactly this oligosaccharide adding proportion
Unexpected technique effect is achieved to the raising of piglet immunological ability.
Embodiment 6:Feeding examination is carried out for Zhejiang Province breeding pig farm, Fujian Province breeding pig farm and Hunan breeding pig farm
It tests, the composite probiotics preparations of 0.2% embodiment of the present invention 1 is added in pig starter feed, observe growth and the diarrhea of piglet
Situation, feedback result show that composite probiotics preparations using the present invention can improve the upgrowth situation of piglet, reduce diarrhea rate
And the death rate, piglet Gain weight are improved, and there is superior technique effect compared to commercially available all kinds of probiotics preparations.
Claims (10)
1. a kind of composite probiotics preparations, which is characterized in that the composite probiotics preparations are by bacillus licheniformis, wine brewing ferment
Mother, animal bifidobacteria, lactobacillus acidophilus, streptococcus thermophilus, tryptophan, xylo-oligosaccharide, galactomannans and zinc lactate group
At.
2. composite probiotics preparations according to claim 1, which is characterized in that the composite probiotics preparations are by following heavy
Amount point at being grouped as:10-15 parts of bacillus licheniformis, 2-4 parts of saccharomyces cerevisiaes, 20-30 parts of animal bifidobacterias, 20-30 parts
Lactobacillus acidophilus, 3-5 part streptococcus thermophilus, 0.0004-0.0006 parts of tryptophans, 20-30 parts of xylo-oligosaccharides, 2-3 portions of galas are sweet
Reveal glycan and 0.00003-0.00008 parts of zinc lactate compositions.
3. composite probiotics preparations according to claim 1, which is characterized in that the composite probiotics preparations are by following heavy
Amount point at being grouped as:12 parts of bacillus licheniformis, 3 parts of saccharomyces cerevisiaes, 25 parts of animal bifidobacterias, 25 parts of lactobacillus acidophilus, 4
Part streptococcus thermophilus, 0.0005 part of tryptophan, 25 parts of xylo-oligosaccharides, 2.5 portions of galactomannans and 0.00005 part of zinc lactate group
At.
4. composite probiotics preparations according to claim 1, which is characterized in that the composite probiotics preparations are by following heavy
Amount point at being grouped as:10 parts of bacillus licheniformis, 2 parts of saccharomyces cerevisiaes, 30 parts of animal bifidobacterias, 20 parts of lactobacillus acidophilus, 3
Part streptococcus thermophilus, 0.0004 part of tryptophan, 20 parts of xylo-oligosaccharides, 2 portions of galactomannans and 0.00008 part of zinc lactate group
At.
5. composite probiotics preparations according to claim 1, which is characterized in that the composite probiotics preparations are by following heavy
Amount point at being grouped as:15 parts of bacillus licheniformis, 3 parts of saccharomyces cerevisiaes, 20 parts of animal bifidobacterias, 30 parts of lactobacillus acidophilus, 5
Part streptococcus thermophilus, 0.0006 part of tryptophan, 30 parts of xylo-oligosaccharides, 3 portions of galactomannans and 0.00003 part of zinc lactate group
At.
6. composite probiotics preparations according to any one of claims 1-5, which is characterized in that wherein lichens gemma bar
Bacterium, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus, streptococcus thermophilus are bacterium powder dosage form, Bacillus licheniformis
Effective viable bacteria content is 1.0 × 10 in powder preparation8CFU/g-2.0×108CFU/g, effectively viable bacteria contains in S. cervisiae powder preparation
Amount is 1.5 × 108CFU/g-2.0×108CFU/g, in animal bifidobacteria bacterium powder preparation effectively viable bacteria content be 1.0 ×
109CFU/g-2.0×109CFU/g, effectively viable bacteria content is 5.0 × 10 in lactobacillus acidophilus bacterium powder preparation8CFU/g-8.0×
108CFU/g, effectively viable bacteria content is 3.0 × 10 in streptococcus thermophilus bacterium powder preparation7CFU/g-5.0×107CFU/g。
7. composite probiotics preparations according to claim 6, which is characterized in that wherein bacillus licheniformis, saccharomyces cerevisiae,
Animal bifidobacteria, lactobacillus acidophilus, streptococcus thermophilus are bacterium powder dosage form, in Bacillus licheniformis powder preparation effectively
Viable bacteria content is 1.5 × 108CFU/g, effectively viable bacteria content is 1.75 × 10 in S. cervisiae powder preparation8CFU/g, animal are double
Effective viable bacteria content is 1.5 × 10 in discrimination bacillus bacterium powder preparation9CFU/g, effective viable bacteria content in lactobacillus acidophilus bacterium powder preparation
It is 6.5 × 108CFU/g, effectively viable bacteria content is 4.0 × 10 in streptococcus thermophilus bacterium powder preparation7CFU/g。
8. the preparation method of the composite probiotics preparations according to any one of claim 1-7, which is characterized in that the system
Preparation Method includes the following steps:Step 1 weighs bacillus licheniformis, saccharomyces cerevisiae, animal bifidobacteria, acidophilus by weight
Lactobacillus and streptococcus thermophilus bacterium powder, tryptophan, xylo-oligosaccharide, galactomannans and zinc lactate;Step 2, by what is weighed
Bacillus licheniformis, saccharomyces cerevisiae, animal bifidobacteria, lactobacillus acidophilus and streptococcus thermophilus bacterium powder, tryptophan, oligomeric wood
Sugar, galactomannans and zinc lactate are uniformly mixed, and are then dispensed, are placed at 4 DEG C and preserve.
9. application of the composite probiotics preparations in pig starter feed described in claim 1-7 any one, which is characterized in that described
Additive amount of the composite probiotics preparations in pig starter feed is 0.2-0.4% (mass percent).
10. a kind of pig starter feed, which is characterized in that be added with the power of 0.2-0.4% mass percentages in the pig starter feed
Profit requires any one of 1-7 composite probiotics preparations.
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CN112042832A (en) * | 2020-09-28 | 2020-12-08 | 井冈山大学 | Poultry breeding microbial feed additive and preparation method and application thereof |
CN112586617A (en) * | 2020-12-08 | 2021-04-02 | 中国科学院亚热带农业生态研究所 | Composite microecological preparation and application thereof in preparation of feed for improving meat quality of Bama miniature pigs |
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