CN104472887A - Probiotic feed additive - Google Patents
Probiotic feed additive Download PDFInfo
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- CN104472887A CN104472887A CN201410847670.7A CN201410847670A CN104472887A CN 104472887 A CN104472887 A CN 104472887A CN 201410847670 A CN201410847670 A CN 201410847670A CN 104472887 A CN104472887 A CN 104472887A
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- bacillus
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- feed additive
- lactobacillus
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- 235000018291 probiotics Nutrition 0.000 title claims abstract description 55
- 239000003674 animal food additive Substances 0.000 title claims abstract description 43
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- Fodder In General (AREA)
Abstract
The invention belongs to the field of biological feed additives, and particularly discloses a probiotic feed additive. The probiotic feed additive is characterized by comprising 20-40% of composite probiotics and 60-80% of fungus chaff protein powders in mass ratio. The composite probiotics comprise the following ingredients: bacillus firmus, bacillus lentus, bacillus coagulans, bacillus subtilis, bacillus licheniformis, streptococcus thermophilus, lactobacillus delbrueckii, lactobacillus acidophilus, lactobacillus bulgaricus, thermophilic bifidobacteria and yeast with the weight ratio of (1.4-2.8):(1.4-2.8):(1.4-2.8):(2.2-4.4):(1.6-3.2):(2.0-4.0):(1.6-3.2):(1.4-2.8):(1.4-2.8):(2.2-4.4):(3.4-6.8). By mixing and matching with fungus chaff protein powders rich in the ingredients of protein, amino acid and polysaccharide, an animal health feed additive is formed; by adding 2-6% of the probiotic feed additive into animal diets, immunity of feeding objects can be effectively enhanced, animal growth can be promoted, the problem of antibiotic residue of animal products is solved, and economic benefits in breeding industry are improved.
Description
Technical field
The present invention relates to biology feed additive field, be specifically related to a kind of probiotic feed additive.
Background technology
Probiotics preparation is a kind of probiotics, being that one applies influential viable microbial feed addictive by improving gut flora balance to animal, according to the development of animal microecology general principle, can be used for regulating animal body microecological balance.It is the class feed addictive suppressing harmful microbe in enteron aisle by strengthening body or carried out prevent disease by enhancing non-specific immune function, promoted growth of animal or improve food conversion ratio.Probiotic feed additive is the active ingredient be made up of living microorganism bacterium, its effect participates in microbial balance in enteron aisle, directly improve efficiency of feed utilization and the speed of growth of animal, as bacillus category mainly produces enzyme, vitamin, amino acid and growth-stimulating factor etc. by living microorganism in enteron aisle, directly reach the object of growth promotion and raising efficiency of feed utilization.As lactic acid bacteria also participates in the balance of microorganism in enteron aisle, but effect is the inhibition (i.e. dominant population effect) increasing animal intestinal microflora, or carry out prevent disease by strengthening non-specific immune function, and indirectly play the effect of growth promotion and raising food utilization efficiency, by improving body non-specific immune function, reducing has or without the enteric infection of clinical symptoms, to having grown indirectly positive role.Probiotic feed additive has no side effect to animal products, noresidue, is of value to green"meat product industrial expansion, therefore probiotic feed additive has been widely used in livestock and poultry and aquaculture industry.
Because probiotic feed additive is active bacteria formulation, stability, the effect relation of pot-life to product of product are very large, with regard to the Tiny ecosystem goods containing profitable probliotics of existing market supply, its charges are mostly wheat bran, corn flour, powder of straw, cereal shell powder, zeolite powder, stone flour, rice bran, beer residue etc. as inserts, the application of these charges have ignored the Adhesion property of its viable bacteria to inserts, cause active bacteria formulation in the unstability of lay up period, cause thalline dead or weak, product can not be made to get a desired effect within the operating period.In addition, the amount ranges of probiotic additive is little, and the ratio of generally adding in feed is in 0.5-5% scope, and its inserts can not embody its nutritive value in feed.
Summary of the invention
The object of the present invention is to provide a kind of new probiotic feed additive, the manufactured goods of the program be not only beneficial to viable bacteria thalline stable, the thalline time-to-live is long, and there is certain Adhesion property, in animal feed, add application simultaneously also there is fine nutritive effect.
The present invention is by compound probiotic and a certain proportion of bacterium chaff albumen powder proportioning, obtain a kind of new probiotic feed additive product, the viable bacteria concentration of this additive is high, thalline is stablized, the thalline time-to-live is long, and the Adhesion property of edible fungus protein powder is good, and also there is the proteinaceous nutrient effect needed for animal.
In order to realize the present invention, specifically have employed following technical scheme:
A kind of probiotic feed additive of the present invention, is characterized in that by mass ratio being that the compound probiotic of 20-40% and the bacterium chaff albumen powder of 60-80% form;
Described compound probiotic, it contains following bacterial classification: bacillus firmus (Bacillus firmus), bacillus lentus (Bacillus lentus), bacillus coagulans (Bacillus coagulans), bacillus subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis) streptococcus thermophilus (Streptococcus thermophilus), Lactobacillus delbrueckii (Lactobacillus delbriickii), lactobacillus acidophilus (Bacillus acidophilus), lactobacillus bulgaricus (Lactobacillusbulgaricus), thermophilic Bifidobacterium Bifidum (Thermophilic Bacillus bifidus) and saccharomycete (Saccharomyces).
Fodder yeast (Candida Tropicali) selected by saccharomycete described above.
The probiotic products that the present invention makes with bacillus and other microbial bacteria proportionings makes a marked difference, bacillus forms gemma in the disadvantageous situation of condition, oneself is protected, resurrection rate is high, within this series products, raw spore form exists, there is more advantage: (1) salt tolerant, high temperature resistant (100 DEG C) and anti-extrusion, thus granulate in the processing of feed, by processes such as animal gastric acid environment in there is higher stability; (2) there is very strong protease, lipase, amylase, sting the activity of the multiple enzyme of matter enzyme etc., use in feed adds, to vegetality carbohydrate, there is stronger degradation capability, in energy degrading plant feed, the compound of some complexity, produces the polypeptides matter with opposing pathogenic entero becteria ability.The carbohydrate of these bacillus to many complexity of plant part has degradation capability, and the material that can be degraded has triglycerides, protein, starch, xylan, pectin, carboxymethyl cellulose, polygalacturonase, araban, glucose, p-nitrophenyl-glucopyranoside, p-nitrophenyl-arabinofuranosidase glucosides, lichenin and p-nitrophenyl-furyl xylose etc.
Yeast cell wall main component is containing multiple nutritional components such as rich in protein, ribonucleic acid, B family vitamin, carbohydrate, cromoci, coacetylase, lipid material, mineral matters in glucan, cell.Can grow in polysaccharide, meta-acid environment, saccharomycete and lactic acid bacteria are also used, on the one hand, product is as feeding conditioning agent, therapeutic agent, certain curative effect is produced to illnesss such as enteritis, diarrhea, constipation, poor appetite, dermatitis, urinary system infection contaminations, as nutritional agents, the gaining effect of livestock and poultry can be improved and then strengthen disease-resistant, the diseases prevention ability of body.On the other hand, except there is " saccharomycete and lactic acid bacteria " to except adjustment for the treatment of effect of livestock and poultry, in adjustment digestive system function is disorderly or as the intensifier under stressed condition, effect is all fairly obvious.Saccharomycete and bacillus composite, according to the biological characteristics of bacterial classification used, it is blendedly joined as feed addictive, the results showed, the growth rate 3.8% of livestock and poultry can be improved.Will " saccharomycete and lactic acid bacteria and bacillus " better effects if after compound in proportion, it is still feeding nutritional agents and feed addictive not, is also feeding therapeutic agent, partly can replaces the treatment of antibiotic for disease of digestive system.
According to the present invention, in order to enable each bacterial classification play more excellent synergy, compound probiotic of the present invention consist of by: bacillus firmus, bacillus lentus, bacillus coagulans, bacillus subtilis, bacillus licheniformis, streptococcus thermophilus, Lactobacillus delbrueckii, lactobacillus acidophilus, lactobacillus bulgaricus, thermophilic Bifidobacterium, saccharomycete order part by weight be:
1.4-2.8:1.4-2.8:1.4-2.8:2.2-4.4:1.6-3.2:2.0-4.0:1.6-3.2: 1.4-2.8:1.4-2.8:2.2-4.4:3.4-6.8, preferred proportion is: 2.1:2.1:2.1:3.3:2.4:3.0:2.4:2.1:2.1:3.3:5.1.
Bacillus firmus described above, bacillus lentus, bacillus coagulans, bacillus subtilis, bacillus licheniformis, streptococcus thermophilus, Lactobacillus delbrueckii, lactobacillus acidophilus, lactobacillus bulgaricus, thermophilic Bifidobacterium, saccharomycete can use all thalline dry powder formulations being no less than 20,000,000,000 as feed addictive every gram viable bacteria concentration commercially available.
Every gram of viable bacteria concentration described above is no less than the bacillus firmus of 20,000,000,000, bacillus lentus, bacillus coagulans, bacillus subtilis, bacillus licheniformis, streptococcus thermophilus, Lactobacillus delbrueckii, lactobacillus acidophilus, lactobacillus bulgaricus, thermophilic Bifidobacterium, saccharomycetic thalline dry powder formulations is not unobtainable by understanding the growth properties of bacterial classification in the prior art, Liquid Culture Zymolysis Equipment can also be adopted separately independently in cultivating system, cultivate bacillus firmus respectively by the culture medium optimized, bacillus lentus, bacillus coagulans, bacillus subtilis, bacillus licheniformis, streptococcus thermophilus, Lactobacillus delbrueckii, lactobacillus acidophilus, lactobacillus bulgaricus, thermophilic Bifidobacterium, saccharomycete, then separates the thalline in zymotic fluid with supercentrifuge, and be placed in dry indoor cold air drying or vacuum freeze drying mode bacterium mud is dry and obtain, acquisition viable bacteria concentration is no less than the bacillus firmus of 20,000,000,000 every gram respectively, bacillus lentus, bacillus coagulans, bacillus subtilis, bacillus licheniformis, streptococcus thermophilus, Lactobacillus delbrueckii, lactobacillus acidophilus, lactobacillus bulgaricus, thermophilic Bifidobacterium, saccharomycete dry powder goods.Can find from reference books as condition of culture (as temperature etc.), as handbook or the book of teaching of the cultivation about microorganism.
Bacterium chaff albumen powder described above is by from production edible mushroom wood sawdust, cotton seed hulls, corncob, wheat bran, rice husk, waste cotton, alditol slag, xylose residue, bagasse, megasse, vinasse, rape cake and multiple kinds of crops powder of straw are the bacterium chaff (also claiming edible mushroom waste residue) after primary raw material culturing edible fungus is raw material, obtain by the following method: first broken for bacterium chaff lobe employing 120 DEG C of heated-air dryings are less than 10% to water content, be ground into 60-80 order powder again, after bacterium chaff powder being added hot-water soak 1-2 hour of weight 10 times amount 40 DEG C, heating boils 1 hour again, then heating is stopped, when temperature is down to below 60 DEG C, adjustment pH is 5.0-6.0, adds the protease of bacterium chaff grain weight amount 1.8-2.2%, the papain of 0.2-0.35% is incubated 56-60 DEG C of hydrolysis after 3-4 hour, then by the heating material after hydrolysis to boiling 30 minutes, adopt squeezer to carry out press filtration, pressing filtering liquid filters to get filtrate with 400 order industrial filter cloths again, filtrate is concentrated through conventional method, dry obtained bacterium chaff albumen powder, the water content of bacterium chaff albumen powder is below 6%.Edible mushroom described above comprises asparagus, pleurotus eryngii, agaricus bisporus, straw mushroom, flat mushroom, mushroom, phoenix-tail mushroom, Pleurotus nebrodensis, Agrocybe chaxingu, true Ji mushroom, meadow dried mushroom, Agaricus bitorqui, Stropharia rugoso-annulata, wizened mushroom, parasol mushroom, Albatrellus dispansus (Lloyd) Canf. & Gilb, black mushroom, HUAZIGU, Lepista sordida, grifola frondosus, Lactrarius hatsudake, Huang Jugu, Huang Liugu, pixie stool, collybia albuminosa, coprinus comatus, T.lobayense Heim, YUHUANGGU, dried mushroom, ferfas, halimasch, cepe, Fistulina hepatica (Schaeff.) Fr, the newborn bacterium of pine, matsutake, dicyclo mushroom, elegant precious mushroom, hickory chick, Russula vinosa, glossy ganoderma, white fungus, black fungus, any one edible mushroom in Hericium erinaceus.
The preparation method of probiotic feed additive of the present invention adopts conventional method, first rear for probiotic mixing and edible fungus protein powder Homogeneous phase mixing is made probiotic feed additive product according to the above ratio.
Beneficial effect of the present invention:
(1) by being rich in protein, amino acid, polysaccharide, vitamin, mineral matter and trace element in the bacterium chaff albumen powder of the chaff material extraction after harvesting edible mushroom, effectively can improve the nutritive value of probio, promote the nutrition improvement of animal, metabolism, improve immunity and the resistance against diseases of body, promote growth of animal.
(2) cohesive utilizing bacterium chaff albumen powder to have, can imitate attachment feed surface and form adhesion layer, add the adhesive effect between thalline and feed, improve the utilization rate of probio.
(3) edible fungus bran albumen powder, as the filler of product, it itself is exactly a kind of nutriment of high protein, be beneficial to the protein raw material of part in supplementary or alternative feed, not only can improve the nutritive effect of feed, save food and to the utilization of fungus chaff wastage, there are very high economic worth and the economic results in society of wasted resources simultaneously.
Product of the present invention integrates probio and trophism protein, can be used as trophism and the use of functional feed addictive, also can as the preparation raw material of other trophism pharmaceutical feeds of preparation.After testing, its gross protein value is respectively 46.24%, 45.61%, 45.79% mean value 45.88% through the testing result of three batches to product of the present invention.The total viable count of the composite probiotics feed additive that the present invention makes is at 2,000,000,000/g-80, hundred million/g, animal intestinal bacterium phase can be changed, set up enteric microorganism district, exogenous pathogeny bacterium has the effect of competitive row's sun, can to the wide spectrum of harmful intestinal tract bacteria, efficient suppression, bactericidal action or by the field planting of direct interference pathogen in enteron aisle, the mode removing pathogen maintains intestinal health, indirectly play growth promotion, when antibiotic-free adds, the immune resistivity of object of feeding can be strengthened, while raising production performance, solve the medicament residue problem of animal product, thus win higher culture benefit.The composite probiotics feed additive made, addition in animal diets is 2-6%, there is good result of use, the composite probiotics feed additive of 3% is added in weanling pig feed, daily gain in pigs improves 50-70g than control group, rate of body weight gain improves 16.93%-23.56%, and feed conversion rate improves 2.1%-9.5%.When adding the composite probiotics feed additive of 6% in the feed of finished cattle, the weightening finish of ox improves 18.5% than control group, and the utilization rate of feed improves 12.7%.Add the composite probiotics feed additive farrowing of 2% in female rabbit daily ration in oestrus, survival rate improves 19.8%-24.5%; Add the composite probiotics feed additive of 2.5% in the feed of young rabbit, daily gain improves 20.5%-34.8%.In chicken feed, add the composite probiotics feed additive of 2%, average weight gain can reach 13.1%, and its appetite can also be made to strengthen, and survival rate improves; Add composite probiotics feed additive in Broiler chicks feed, can improve weightening finish 8.1%-11.9%, improve food conversion ratio 12.1%, improve disease-resistant, anti-scaring ability, survival rate improves 6.1%; Add the composite probiotics feed additive of 3% in egg feedstuff, all have obvious enhancement effect to food conversion, resistance against diseases etc., laying rate improves 1.15%.In carp feed, add the composite probiotics feed additive of 5%, carp body weight improves 16%, and per mu yield improves 10.6%, and flesh of fish free aminoacid content improves 16%-26%.
A kind of composite probiotics feed additive of the present invention, has good stability, through 3 batches of reserved sample observing results: the total viable count of thalline respectively: within 3 months, survival rate is 98.2%; Within 6 months, survival rate is 96.6%; Within 12 months, survival rate is 92.4%.As can be seen here, composite probiotics feed additive of the present invention can improve and the survival rate of stable probio preferably, can ensure that product plays the validity of viable bacteria in storage life, thus ensures use effect of product.
Unless otherwise indicated, its percentage is all weight percentage in the present invention.
Detailed description of the invention
Following examples are only further illustrate of the present invention; but not be all of the present invention; below, preferred embodiment to one skilled in the art, under the prerequisite not departing from the present invention program's know-why, the improvements and modifications of carrying out all are considered as protection scope of the present invention.
The preparation of bacterium chaff albumen powder, the present embodiment, according to the source of raw material, can select the bacterium chaff raw material of any one culturing edible fungus, also can select for a post two or more Mixed Microbes chaff raw material of meaning as the raw materials of bacterium chaff albumen powder.
Embodiment 1
Get mycelia pure white, material agllutination is real, without going mouldy, the edible mushroom asparagus wood chip bacterium chaff lobe rotted is broken, adopt dryer, 10% is less than to water content in 120 DEG C of heated-air dryings, after cooling, Universalpulverizer is adopted to pulverize, cross 80 mesh sieves, obtain bacterium chaff powder, get 10 kilograms, bacterium chaff powder, add the hot water of 100 kilograms 40 DEG C, stir evenly, place and soak after 1-2 hour, heating boils 1 hour again, then heating is stopped, feed temperature is made to be down to less than 60 DEG C, adjustment pH is 5.0, add the protease of 200 grams and the papain of 30 grams, stir, be incubated 56 DEG C of enzymolysis after 4 hours, be heated to boil 30 minutes again, filter press is adopted to carry out press filtration enzymolysis material, pressing filtering liquid filters through 400 order industrial filter cloths, by filtrate with vacuum rotary evaporator at temperature 60 C, concentrate under vacuum-0.08MPa condition, being concentrated into concentrate proportion is 1.3, and then spray-dried obtained bacterium chaff albumen powder product.Product water content is 4.5%; Detect by GB/T 18868-2002 method, gross protein value is 46.24%.
Embodiment 2
Get mycelia pure white, material agllutination is real, without going mouldy, the edible mushroom pleurotus eryngii corncob bacterium chaff lobe rotted is broken, adopt dryer, 10% is less than to water content in 120 DEG C of heated-air dryings, after cooling, Universalpulverizer is adopted to pulverize, cross 60 mesh sieves, obtain bacterium chaff powder, get 10 kilograms, bacterium chaff powder, add the hot water of 100 kilograms 40 DEG C, stir evenly, place and soak after 1-2 hour, heating boils 1 hour again, then heating is stopped, feed temperature is made to be down to less than 60 DEG C, adjustment pH is 6.0, add the protease of 210 grams and the papain of 24 grams, stir insulation 60 DEG C of enzymolysis after 3 hours, be heated to boil 30 minutes again, filter press is adopted to carry out press filtration enzymolysis material, pressing filtering liquid filters through 400 order industrial filter cloths, filtrate atmospheric evaporation is concentrated into paste, then under 70 DEG C of conditions, with drying box heated-air drying 40 hours obtained bacterium chaff albumen powder products.Product water content is 5.6%; Detect by GB/T 18868-2002 method, gross protein value is 45.61%.
Embodiment 3
Get mycelia pure white, material agllutination is real, without going mouldy, rot edible mushroom glossy ganoderma wood chip and corncob Mixed Microbes chaff lobe broken, adopt dryer, 10% is less than to water content in 120 DEG C of heated-air dryings, after cooling, Universalpulverizer is adopted to pulverize, cross 70 mesh sieves, obtain bacterium chaff powder, get 10 kilograms, bacterium chaff powder, add the hot water of 100 kilograms 40 DEG C, stir evenly, place and soak after 1-2 hour, heating boils 1 hour again, then heating is stopped, feed temperature is made to be down to less than 60 DEG C, adjustment pH is 5.5, add the protease of 190 grams and the papain of 26 grams, stir insulation 58 DEG C of enzymolysis after 3.5 hours, be heated to boil 30 minutes again, filter press is adopted to carry out press filtration enzymolysis material, pressing filtering liquid filters through 400 order industrial filter cloths, filtrate atmospheric evaporation is concentrated into paste, then under 80 DEG C of conditions, with the dry 34 hours obtained bacterium chaff albumen powder products of hot air drier.Product water content is 5.4%; Detect by GB/T 18868-2002 method, gross protein value is 45.79%.
Embodiment 4
Use corresponding Optimal Medium, individually bacillus firmus is cultivated by liquid fermentation equipment, bacillus lentus, bacillus coagulans, bacillus subtilis, bacillus licheniformis, streptococcus thermophilus, Lactobacillus delbrueckii, lactobacillus acidophilus, lactobacillus bulgaricus, thermophilic Bifidobacterium and fodder yeast, then with supercentrifuge, the thalline in zymotic fluid is separated, by vacuum freeze drying mode by dry for centrifugal bacterium mud out, to obtain viable bacteria content be 20,000,000,000/gram bacillus firmus dry powder, viable bacteria content is 20,000,000,000/gram bacillus lentus dry powder, viable bacteria content is 20,000,000,000/gram bacillus coagulans dry powder, viable bacteria content is 20,000,000,000/gram bacillus subtilis dry powder, viable bacteria content is 20,000,000,000/gram bacillus licheniformis dry powder, viable bacteria content is 20,000,000,000/gram streptococcus thermophilus dried powder, viable bacteria content is 20,000,000,000/gram Lactobacillus delbrueckii dry powder, viable bacteria content is 20,000,000,000/gram lactobacillus acidophilus dry powder, viable bacteria content is 20,000,000,000/gram lactobacillus bulgaricus dry powder, viable bacteria content is 20,000,000,000/gram thermophilic bifidobacteria dry powder and viable bacteria content be 20,000,000,000/gram feed yeast powder.
Embodiment 5
Take 2.1 kilograms, the bacillus firmus dry powder that embodiment 4 obtains, 2.1 kilograms, bacillus lentus dry powder, 2.1 kilograms, bacillus coagulans dry powder, 3.3 kilograms, bacillus subtilis dry powder, 2.4 kilograms, bacillus licheniformis dry powder, streptococcus thermophilus dried powder 3.0 kilograms, 2.4 kilograms, Lactobacillus delbrueckii dry powder, 2.1 kilograms, lactobacillus acidophilus dry powder, 2.1 kilograms, lactobacillus bulgaricus dry powder, thermophilic bifidobacteria dry powder 3.3 kilograms and feed yeast powder 5.1 kilograms are placed in stirrer for mixing and become 30 kilograms, composite thallus dry powder, take bacterium chaff albumen powder 70 kilograms that embodiment 1 obtains again to add in above-mentioned composite thallus dry powder and continue to stir, after fully mixing, pack, obtain probiotic feed additive product of the present invention.
Embodiment 6
Take 1.4 kilograms, the bacillus firmus dry powder that embodiment 4 obtains, 1.4 kilograms, bacillus lentus dry powder, 1.4 kilograms, bacillus coagulans dry powder, 2.2 kilograms, bacillus subtilis dry powder, 1.6 kilograms, bacillus licheniformis dry powder, streptococcus thermophilus dried powder 2.0 kilograms, 1.6 kilograms, Lactobacillus delbrueckii dry powder, 1.4 kilograms, lactobacillus acidophilus dry powder, 1.4 kilograms, lactobacillus bulgaricus dry powder, thermophilic bifidobacteria dry powder 2.2 kilograms and feed yeast powder 3.4 kilograms are placed in stirrer for mixing and become 20 kilograms, composite thallus dry powder, take bacterium chaff albumen powder 80 kilograms that embodiment 2 obtains again to add in above-mentioned composite thallus dry powder and continue to stir, after fully mixing, pack, obtain probiotic feed additive product of the present invention.
Embodiment 7
Take 2.8 kilograms, the bacillus firmus dry powder that embodiment 4 obtains, 2.8 kilograms, bacillus lentus dry powder, 2.8 kilograms, bacillus coagulans dry powder, 4.4 kilograms, bacillus subtilis dry powder, 3.2 kilograms, bacillus licheniformis dry powder, streptococcus thermophilus dried powder 4.0 kilograms, 3.2 kilograms, Lactobacillus delbrueckii dry powder, 2.8 kilograms, lactobacillus acidophilus dry powder, 2.8 kilograms, lactobacillus bulgaricus dry powder, thermophilic bifidobacteria dry powder 4.4 kilograms and feed yeast powder 6.8 kilograms are placed in stirrer for mixing and become 40 kilograms, composite thallus dry powder, take bacterium chaff albumen powder 60 kilograms that embodiment 3 obtains again to add in above-mentioned composite thallus dry powder and continue to stir, after fully mixing, pack, obtain probiotic feed additive product of the present invention.
Embodiment 8
Take 2.8 kilograms, the bacillus firmus dry powder that embodiment 4 obtains, 2.3 kilograms, bacillus lentus dry powder, 1.8 kilograms, bacillus coagulans dry powder, 3.5 kilograms, bacillus subtilis dry powder, 2.8 kilograms, bacillus licheniformis dry powder, streptococcus thermophilus dried powder 3.8 kilograms, 3 kilograms, Lactobacillus delbrueckii dry powder, 2.5 kilograms, lactobacillus acidophilus dry powder, 2.5 kilograms, lactobacillus bulgaricus dry powder, thermophilic bifidobacteria dry powder 4.0 kilograms and feed yeast powder 6.0 kilograms are placed in stirrer for mixing and become 35 kilograms, composite thallus dry powder, take bacterium chaff albumen powder 70 kilograms that embodiment 1 obtains again to add in above-mentioned composite thallus dry powder and continue to stir, after fully mixing, pack, obtain probiotic feed additive product of the present invention.
Embodiment 9
Take 1.75 kilograms, the bacillus firmus dry powder that embodiment 4 obtains, 1.75 kilograms, bacillus lentus dry powder, 1.75 kilograms, bacillus coagulans dry powder, 2.75 kilograms, bacillus subtilis dry powder, 2.0 kilograms, bacillus licheniformis dry powder, streptococcus thermophilus dried powder 2.5 kilograms, 2.0 kilograms, Lactobacillus delbrueckii dry powder, 1.75 kilograms, lactobacillus acidophilus dry powder, 1.75 kilograms, lactobacillus bulgaricus dry powder, thermophilic bifidobacteria dry powder 2.75 kilograms and feed yeast powder 4.25 kilograms are placed in stirrer for mixing and become 25 kilograms, composite thallus dry powder, take bacterium chaff albumen powder 75 kilograms that embodiment 2 obtains again to add in above-mentioned composite thallus dry powder and continue to stir, after fully mixing, pack, obtain probiotic feed additive product of the present invention.
Claims (8)
1. a probiotic feed additive, is characterized in that by mass ratio being that the compound probiotic of 20-40% and the bacterium chaff albumen powder of 60-80% form.
2. probiotic feed additive as claimed in claim 1, it is characterized in that, described compound probiotic, Mixed Microbes containing following bacterial classification: bacillus firmus (Bacillus firmus), bacillus lentus (Bacillus lentus), bacillus coagulans (Bacillus coagulans), bacillus subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis) streptococcus thermophilus (Streptococcus thermophilus), Lactobacillus delbrueckii (Lactobacillus delbriickii), lactobacillus acidophilus (Bacillus acidophilus), lactobacillus bulgaricus (Lactobacillusbulgaricus), thermophilic Bifidobacterium Bifidum (Thermophilic Bacillus bifidus) and saccharomycete (Saccharomyces).
3. probiotic feed additive as claimed in claim 2, it is characterized in that, described saccharomycete is fodder yeast (Candida Tropicali).
4. the probiotic feed additive as described in any one of claim 2-3, it is characterized in that, the composition of described compound probiotic is pressed: the part by weight of bacillus firmus, bacillus lentus, bacillus coagulans, bacillus subtilis, bacillus licheniformis, streptococcus thermophilus, Lactobacillus delbrueckii, lactobacillus acidophilus, lactobacillus bulgaricus, thermophilic Bifidobacterium, saccharomycete order is respectively: 1.4-2.8:1.4-2.8:1.4-2.8:2.2-4.4:1.6-3.2:2.0-4.0:1.6-3.2: 1.4-2.8:1.4-2.8:2.2-4.4:3.4-6.8.
5. probiotic feed additive as claimed in claim 4, it is characterized in that, the composition of described compound probiotic is pressed: the part by weight of bacillus firmus, bacillus lentus, bacillus coagulans, bacillus subtilis, bacillus licheniformis, streptococcus thermophilus, Lactobacillus delbrueckii, lactobacillus acidophilus, lactobacillus bulgaricus, thermophilic Bifidobacterium, saccharomycete order is respectively: 2.1:2.1:2.1:3.3:2.4:3.0:2.4:2.1:2.1:3.3:5.1.
6. the probiotic feed additive as described in claim 4 or 5, it is characterized in that, described bacillus firmus, bacillus lentus, bacillus coagulans, bacillus subtilis, bacillus licheniformis, streptococcus thermophilus, Lactobacillus delbrueckii, lactobacillus acidophilus, lactobacillus bulgaricus, thermophilic Bifidobacterium, saccharomycete, adopt every gram of viable bacteria concentration to be the thalline dry powder goods of 20,000,000,000.
7. probiotic feed additive as claimed in claim 1, is characterized in that, described bacterium chaff albumen powder is by from production edible mushroom wood sawdust, cotton seed hulls, corncob, wheat bran, rice husk, waste cotton, alditol slag, xylose residue, bagasse, megasse, vinasse, rape cake and multiple kinds of crops powder of straw are the bacterium chaff after primary raw material culturing edible fungus is raw material, obtain by the following method: first broken for bacterium chaff lobe employing 120 DEG C of heated-air dryings are less than 10% to water content, be ground into 60-80 order powder again, after bacterium chaff powder being added hot-water soak 1-2 hour of weight 10 times amount 40 DEG C, heating boils 1 hour again, then stops heating, when temperature is down to below 60 DEG C, adjustment pH is 5.0-6.0, adds the protease of bacterium chaff grain weight amount 1.8-2.2%, the papain of 0.2-0.35% is incubated 56-60 DEG C of hydrolysis after 3-4 hour, then by the heating material after hydrolysis to boiling 30 minutes, adopt squeezer to carry out press filtration, pressing filtering liquid filters to get filtrate with 400 order industrial filter cloths again, filtrate is concentrated through conventional method, dry obtained bacterium chaff albumen powder, the water content of bacterium chaff albumen powder is below 6%, described edible mushroom comprises asparagus, pleurotus eryngii, agaricus bisporus, straw mushroom, flat mushroom, mushroom, phoenix-tail mushroom, Pleurotus nebrodensis, Agrocybe chaxingu, true Ji mushroom, meadow dried mushroom, Agaricus bitorqui, Stropharia rugoso-annulata, wizened mushroom, parasol mushroom, Albatrellus dispansus (Lloyd) Canf. & Gilb, black mushroom, HUAZIGU, Lepista sordida, grifola frondosus, Lactrarius hatsudake, Huang Jugu, Huang Liugu, pixie stool, collybia albuminosa, coprinus comatus, T.lobayense Heim, YUHUANGGU, dried mushroom, ferfas, halimasch, cepe, Fistulina hepatica (Schaeff.) Fr, the newborn bacterium of pine, matsutake, dicyclo mushroom, elegant precious mushroom, hickory chick, Russula vinosa, glossy ganoderma, white fungus, black fungus, any one in Hericium erinaceus.
8. probiotic feed additive according to claim 1, is characterized in that: the addition of additive is 2-6% in animal diets.
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| CN111758834A (en) * | 2019-10-16 | 2020-10-13 | 山东九一生物科技股份有限公司 | Special strain formula for livestock and poultry breeding industry |
| CN112219937A (en) * | 2020-10-25 | 2021-01-15 | 江苏小禾生物技术研究院有限公司 | Feed additive |
| CN115287200A (en) * | 2022-08-19 | 2022-11-04 | 景洪宏臻农业科技有限公司 | Rapid screening method of boletus nigricans mutant strains |
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