CN104472883A - Liquid biological feed additive - Google Patents
Liquid biological feed additive Download PDFInfo
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- CN104472883A CN104472883A CN201410832515.8A CN201410832515A CN104472883A CN 104472883 A CN104472883 A CN 104472883A CN 201410832515 A CN201410832515 A CN 201410832515A CN 104472883 A CN104472883 A CN 104472883A
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Abstract
The invention discloses a liquid biological feed additive. The liquid biological feed additive comprises the following materials by weight ratio: 10-20% of beer yeast, 8-15% of lactic acid bacteria, and 72-82% of an edible mushroom bran extraction concentrated solution. The liquid biological feed additive is a novel liquid biological feed additive obtained by mixing the beer yeast, the lactic acid bacteria and a certain proportion of the mushroom bran extraction concentrated solution, is stable in thallus and long in thallus surviving time, extracts and concentrates the mushroom bran rich in nutrient substances of protein, amino acid, polyose, trace elements and the like as a solvent, has relatively high nutritional value, is beneficial to improvement of appetite of animals, promotes nutritive absorption and growth of the animals, enhances disease-resistant and disease prevention capability of bodies, can partially replace antibiotics to be used for prevention and cure of digestive system diseases in practical application, and improves livestock breeding economic benefits.
Description
Technical field
The present invention relates to a kind of feed addictive, specifically a kind of liquid-type biology feed additive.
Background technology
Probiotics preparation is a kind of probiotics, being that one applies influential viable microbial feed addictive by improving gut flora balance to animal, according to the development of animal microecology general principle, can be used for regulating animal body microecological balance.It is the class feed addictive suppressing harmful microbe in enteron aisle by strengthening body or carried out prevent disease by enhancing non-specific immune function, promoted growth of animal or improve food conversion ratio.Probiotic feed additive is the active ingredient be made up of living microorganism bacterium, its effect participates in microbial balance in enteron aisle, directly improve efficiency of feed utilization and the speed of growth of animal, as bacillus category mainly produces enzyme, vitamin, amino acid and growth-stimulating factor etc. by living microorganism in enteron aisle, directly reach the object of growth promotion and raising efficiency of feed utilization.Probiotic feed additive has no side effect to animal products, noresidue, is of value to green"meat product industrial expansion, therefore probiotic feed additive has been widely used in livestock and poultry and aquaculture industry.
Edible fungus bran utilizes wood chip, cotton seed hulls, corncob, wheat bran, rice husk, waste cotton, alditol slag, xylose residue, bagasse, megasse, vinasse, rape cake and multiple kinds of crops powder of straw to carry out edible mushroom substituting stuff cultivation for primary raw material, culture medium residue after results, is commonly called as edible fungus culturing waste material, bacterium slag or clout; It is the compound of the compositions such as the crude fibre containing the residual body of hypha of edible fungus and edible mushroom enzymolysis structure generation qualitative change.China's edible and medical fungi cultivation industry development is rapid, and industry size is huge, is Edible Fungi big country of the world, is also Edible fungi exportation big country, only edible mushroom annual production about 1,100 ten thousand tons in the world.Edible and medical fungi remains cultivation base stock-i.e. bacterium chaff postpartum, and about have 20% to be converted into fructification in every hundred kilograms of siccatives, 20% comparatively becomes thermal energy consumption, and its quantity is edible and medical fungi compost 60%, is about 6,000,000 tons.Bacterium chaff has mycelium to grow in a large number, nutritive value is higher, analyze according to there being scholar, in edible fungus bran, gross protein value is about 2%-12%, crude fat average out to 0.8%-1.2%, crude fibre is on average about 14%-23%, simultaneously also containing abundant mineral matter element and the trace element such as calcium, phosphorus, zinc, copper, iron, magnesium.Medicinal fungus is as Ganoderma Lucidum sugar, and through Ganoderma Lucidum degradation, crude fibre is degraded to 23.0% from 34.5% of Medium for Ganoderma lucidum material, reduces 11.5%, and crude protein is increased to 10.2% from 5.0%, increases by 5.2%; Crude fat is increased to 4.7% from 0.40%; Crude fibre significantly reduces, and nutritive value increases substantially, dry gross energy 3.38 thermie/kilogram.For pig, digestible energy can reach 2.54 thermies/kilogram, close with the digestible energy of wheat skin, be equivalent to corn digestible energy 75%, but crude protein content is higher than corn and Mai Pi.
According to edible fungus bran contain fiber more, decompose not exclusively, many deficiency factors such as palatability is poor, nutritional utilization is insufficient, with regard to how to obtain, feed nutrient is high, fiber hydrolization is more complete, the nutrient composition content such as protein, fat is high, and edible fungus bran being obtained efficiently, fully utilizes fully is the problem that will solve at present.For this reason, the beneficiating ingredient of edible and medical fungi bacterium chaff is effectively extracted, again extract is carried out concentrated adding saccharomycete, lactic acid bacteria is re-dubbed liquid-type biology feed additive, the problem that bacterium chaff fiber is difficult to absorb can not only be solved, significantly can promote the content of mycoprotein simultaneously, improve nutritive value and palatability.Bacterium chaff water extracting liquid and saccharomycete and lactic acid bacteria are carried out proportioning, effectively can promote the efficiency utilization of bacterium chaff, improve the palatability of animal, make edible fungus bran provide a kind of effective approach as liquid-type feed applications.In existing disclosed patented technology, apply as nutrient feed additive with regard to edible fungus bran extract, disclose livestock and poultry edible fungus bran probiotics-containing nutrient solution and preparation method thereof (CN101731460A), its technical scheme is that edible fungus bran is carried 2-3 hour through poach, isolated by filtration gets filtrate, then bacterium chaff extract is added other nutriment more respectively and prepare fermentation culture I and II respectively, add in bacterium chaff digestion liquid by fermentation culture respectively again, within 5-7 days, fermentation culture I is obtained 32-37 DEG C of cultivation, again fermentation culture II is added in bacterium bacterium digestion liquid, within 2-3 days, fermentation culture II is obtained 24-28 DEG C of cultivation, and then fermentation culture I and II is mixed according to volume ratio 2:1, packing is made, can find out from prior art and utilize the edible fungus bran digestion liquid probiotics-containing nutrient solution made of fermented and cultured respectively, the metabolite that its bacterial classification produces after fermented and cultured can improve the nutritive effect of its nutrient solution, but it is imprecise with regard to its probio content, after fermentation, packing makes the easy aging decline of bacterial classification be its defect, the effect of probio contained by nutrient solution can not be ensured.
Summary of the invention
The object of the present invention is to provide a kind of new liquid-type biology feed additive, the charges trophism that this feed addictive not only overcomes existing common solid type feed addictive conventional is poor, the defect of quality instability is also different from existing employing liquid-type bacterium chaff extract and carries out the method for fermentation for feed addictive, probio and a certain proportion of edible fungus bran are extracted concentrate to mix by the present invention, obtain a kind of new liquid-type biology feed additive, this additive is not only thalline and is stablized, the thalline time-to-live is long, and the bacterium chaff that excipient is high protein extracts concentrate, its protein, amino acid, polysaccharide and micronutrient levels enrich, there is higher nutritive value, this additive, directly can add in animal feed and use, good by its adhesion of spice, make probio can be bonded in feed surface equably, thus improve the utilization rate of probio, can also be used for preparing drinking agent, animal is passed through to drink water the nutriment reaching and provide probio and solvent carrier, to improve the result of use of probio.
The present invention is achieved by the following technical solutions:
A kind of liquid-type biology feed additive, it is characterized in that by percentage by weight be 10-20% brewer's yeast, 8-15% lactic acid bacteria, 72-82% edible fungus bran extract concentrate and form, obtain by the following method:
(1) by the edible fungi asparagus after 2-3 stubble of gathering, pleurotus eryngii, agaricus bisporus, straw mushroom, flat mushroom, mushroom, phoenix-tail mushroom, Pleurotus nebrodensis, Agrocybe chaxingu, true Ji mushroom, meadow dried mushroom, Agaricus bitorqui, Stropharia rugoso-annulata, wizened mushroom, parasol mushroom, Albatrellus dispansus (Lloyd) Canf. & Gilb, black mushroom, HUAZIGU, Lepista sordida, grifola frondosus, Lactrarius hatsudake, Huang Jugu, Huang Liugu, pixie stool, collybia albuminosa, coprinus comatus, T.lobayense Heim, YUHUANGGU, dried mushroom, ferfas, halimasch, cepe, Fistulina hepatica (Schaeff.) Fr, the newborn bacterium of pine, matsutake, dicyclo mushroom, elegant precious mushroom, hickory chick, Russula vinosa, glossy ganoderma, white fungus, black fungus, any one bacterium chaff in Hericium erinaceus or two or more Mixed Microbes chaff are selected in earnest, get mycelia pure white, material agllutination is real, without going mouldy, without the part of rotting, smash, drying, pulverize, edible fungus bran powder after pulverizing crosses 250-300 mesh sieve, obtain bacterium chaff powder,
(2) in the bacterium chaff powder of step (1), add 30-40 DEG C, concentration is the ethanol of 60-90%, bacterium chaff and ethanol weight ratio are 1:10-30, soak after 1-6 hour, then are heated to 50-60 DEG C of refluxing extraction 12-36 hour;
(3) squeezed by the material after step (2) refluxing extraction, obtain squeezing juice and bacterium chaff slag, squeeze the juice and to filter with 400 order industrial filter cloths, filtrate recycling ethanol, obtained extract is stand-by;
(4) water bacterium chaff slag in step (3) being added again bacterium chaff powder weight ratio 5-10 times amount is heated to 95-100 DEG C of refluxing extraction 6-15 hour, then squeezes, and obtains squeezing juice; Bacterium chaff slag is separately used as him;
(5) by the squeezing juice centrifugation that step (4) obtains, centrifugal rotational speed 9000-16000rpm, obtains supernatant;
(6) supernatant that extract step (3) obtained and step (5) obtain merges, 60-90 DEG C, to be concentrated into proportion under-0.05MPa ~-0.1MPa condition be 1.15-1.20, obtain edible fungus bran and extract concentrate;
(7) the concentrate adjustment pH to 6.2-7.0 step (6) obtained, in 0.7kg/cm
2, sterilizing 35min under 115 DEG C of conditions;
(8) concentrate after step (7) sterilizing is naturally cooled to less than 35 DEG C, take the concentrate of formula ratio and the brewer's yeast of formula ratio and lactic acid bacteria mixing and stirring, filling in the vial after sterilization treatment or plastic bottle, sealing, obtains product.
According to the present invention, do to optimize further and/or select to technique scheme:
Lactic acid bacteria (Lactobacillus) described above is streptococcus thermophilus (Streptococcus thermophilus), streptococcus lactis (Streptococcus acidi lactici), Pediococcus acidilactici (Pediococcus acidilactici), Lactobacillus plantarum (Lactobacillus plantarum), any one or two kinds and above any combination in lactobacillus bulgaricus (Lactobacillus bulgaricus) and Bifidobacterium (Bacillus bifidus).
Brewer's yeast described above and lactic acid bacteria can select viable bacteria concentration every gram commercially available to be no less than the thalline dry powder formulations of 20,000,000,000.Also existing method can be adopted to prepare, brewer's yeast such as described above and the dry powder of lactic acid bacteria can be cultivated respectively by liquid fermentation equipment, then with supercentrifuge, the thalline in fermentation is separated, be placed in dry indoor vacuum freeze drying mode by obtained for the drying of bacterium mud, the viable bacteria concentration every gram of the thalline dry powder of acquisition is no less than 20,000,000,000.
Because liquid-type biology feed additive of the present invention is liquid preparation, if the brewer's yeast adopted and lactic acid bacteria treat the used time for showing with but not preserving, also existing method can be adopted as above-mentioned brewer's yeast and lactic acid bacteria cultivated respectively by liquid fermentation equipment, then with supercentrifuge, the thalline in fermentation is separated, be diluted to SPSS the liquid product that viable bacteria concentration every gram is no less than 20,000,000,000.
Thalline dry powder goods described above or the weight proportion of liquid product in process for preparation are determined by the dry powder of thalline or the thalline content of liquid product final products.
Its matrix of edible fungus bran described above is one or more the mixture in cotton seed hulls, peanut shell, wood chip, rice husk, corncob, agricultural crop straw.
Described in above-mentioned steps (1) oven dry be adopt the low temperature hot-air seasoning 8-16 hour of 150-400 DEG C; Described pulverizing is ultramicro grinding, preferably uses horizontal continuous stirring micronizer.
In above-mentioned steps (2), the weight ratio of bacterium chaff and ethanol is preferably 1:10-15; Preferred concentration of alcohol is 60-80%; Preferred reflux extracting time is 26-36 hour.
Packaging type squeezer is selected in above-mentioned steps (3) and the squeezing described in step (4); Described in step (4), preferably horizontal spiral centrifuge is used to squeezing juice centrifugation; Preferred centrifugal rotational speed 9000-11000rpm.
In above-mentioned steps (6), preferred concentrated condition is 65-75 DEG C ,-0.065MPa ~-0.085MPa.
Concentrated preferably use vacuum rotary evaporator or plate evaporation inspissator described in above-mentioned steps (6).
The above-described equipment of the present invention is this area conventional equipment.
Do not state the operation of its technical process in processing step in the present invention in detail, be the routine operation in the general knowledge of those skilled in the art.
Except as otherwise noted, percentage of the present invention is all weight percentage.
Wherein lactic acid bacteria has good inhibitory action to some spoilage organisms and psychrotropic bacteria.Can be used for preventing and treating diarrhoea, diarrhea, enteritis, constipation and the various diseases caused because function of intestinal canal is disorderly and scytitis (Zhao Hongxia, 2003).Its antibacterial mechanisms is mainly manifested in the following aspects: the organic acids such as the lactic acid 1. produced significantly can reduce environmental pH and Eh (oxidation-reduction potential) value, make to be in sour environment in intestines, for pathogenic bacteria as shigella dysenteriae, typhoid bacillus, paratyphosum Bacterium, campylobacter, staphylococcus etc. have antagonism; 2. the hydrogen peroxide produced can activate " catalase-thiocyanic acid " system in cow's milk, suppresses and kills Gram-negative bacteria, catalase positive bacterium as pseudomonas, coliform bacteria and Salmonella etc.; 3. produce tiny protein or the peptide class (antibacterial peptide) of similar bacteriocin, as various lactobacillin and bifid rhzomorph, to staphylococcus, shuttle shape gemma bar, wealthy and salmonella and Shigella have antagonism.
In actual use, liquid-type biology feed additive and feed are fully mixed, stir, probio is made evenly to be bonded in feed surface, the utilization rate of probio can be improved, also may be used for preparation non-antibiotic class as pharmaceutical feeds such as nutrient drug such as vitamins, the adhesive effect that can increase between medicine and feed improves the utilization rate of medicine.In actual use, make an addition in drinking water for animals, animal can be made directly to reach the object of raising food probio and the nutritional supplementation improving animal by drinking-water.
The present invention has following characteristics:
(1) liquid-type biology feed additive of the present invention has the characteristic of high protein, high viable bacteria, and through sampling observation, by GB/T 6432 standard detection, wherein crude protein content is at 48-64%.In product, total viable bacteria content is at 3,600,000,000-56 hundred million/gram, because main auxiliary base-material of the present invention is that edible fungus bran adopts concentration to be ethanol and the water refluxing extraction respectively of 60-90%, effectively the composition being soluble in organic solvent and water effectively can be extracted respectively, be 1.15-1.20 through being concentrated into proportion again, its vegetal pole horn of plenty, be rich in the protein, amino acid, polysaccharide and the various trace elements that are rich in mycelium, abundant nutrition can be provided to animal, add brewer's yeast, improve the content of B family vitamin, saccharomycete is rich in Cobastab
1, B
2, B
6, B
12and the physiological activator such as wheat sterol, nicotinic acid, folic acid, pantothenic acid, inositol, saccharomyces cerevisiae and lactic acid bacteria compound use, it is good probio, product is as feed conditioning agent, therapeutic agent on the one hand, certain curative effect is produced to enteritis diarrhea, constipation, poor appetite etc., as nutritional agents, the gaining effect of livestock and poultry can be improved, and then strengthen disease-resistant, the diseases prevention ability of body.Not only be of value to raising animal appetite, promote absorption of nutrient ingredients, impel growth of animal, partly can replace the control of antibiotic for disease of digestive system in actual applications.
(2) because the auxiliary base-material of product of the present invention is that to be concentrated into proportion through second alcohol and water refluxing extraction concentrate be respectively 1.15-1.20 to edible fungus bran, the toughness of its body fluid is comparatively large, can significantly improve the survival rate of thalline.
(3) product of the present invention and feed are fully mixed stirring, thalline uniform adhesion can be made at feed surface, the utilization rate of probio can be improved; Can be used for preparation non-antibiotic as the pharmaceutical feed of the nutrition such as vitamins etc. simultaneously, the adhesive effect between medicine and feed can be increased, improve utilization ratio of drug, liquid-type biology feed additive of the present invention, directly can add in animal drinking water and apply, can the practical application of improving product effectively.When preparing burden as drinking water for animals agent, prepare after drinking water leaves standstill 3-5 hour and use, microbial activity can be made to bring back to life, and thalline quantity obviously increases, and more promotes result of use.
(4) product of the present invention is different from prior art and utilizes bacterium chaff Aqueous extracts to ferment to cause bacterial classification easily aging, decline again, cannot quantize and the shortcoming such as storage life is short, product of the present invention was through reserved sample observing 12 months, its thalline survival rate is still greater than 96%, proves that its product has good stability.
(5) utilize edible fungus bran through second alcohol and water refluxing extraction concentrate, mycoprotein, amino acid, polysaccharide, vitamin and various trace elements and macroelement has been rich in its concentrate, the nutritive value of further raising probio, promote the metabolism of animal, improve resistance against diseases, the develop immunitypty of body, promote growth of animal, finally improve economic benefit.
Detailed description of the invention
Following examples further illustrate of the present invention, should not regard limitation of the present invention as.
Embodiment 1
Use corresponding Optimal Medium, individually brewer's yeast is cultivated by liquid fermentation equipment, streptococcus thermophilus, streptococcus lactis, Pediococcus acidilactici, Lactobacillus plantarum, lactobacillus bulgaricus and Bifidobacterium, then with supercentrifuge, the thalline in zymotic fluid is separated, by vacuum freeze drying mode by dry for centrifugal bacterium mud out, to obtain viable bacteria content be 20,000,000,000/gram brewer's yeast dry powder, viable bacteria content is 20,000,000,000/gram streptococcus thermophilus dried powder, viable bacteria content is 20,000,000,000/gram streptococcus lactis dry powder, viable bacteria content is 20,000,000,000/gram Pediococcus acidilactici dry powder, viable bacteria content is 20,000,000,000/gram plant lactobacillus powder, viable bacteria content is 20,000,000,000/gram lactobacillus bulgaricus dry powder and viable bacteria content be 20,000,000,000/gram bifidobacteria dry powder.
Embodiment 2
Use corresponding Optimal Medium, individually brewer's yeast is cultivated by liquid fermentation equipment, streptococcus thermophilus, streptococcus lactis, Pediococcus acidilactici, Lactobacillus plantarum, lactobacillus bulgaricus and Bifidobacterium, then with supercentrifuge, the thalline in zymotic fluid is separated, centrifugal bacterium mud out to be adjusted to viable bacteria content with SPSS be 20,000,000,000/gram saccharomyces cerevisiae liquid, viable bacteria content is 20,000,000,000/gram streptococcus thermophilus bacterium liquid, viable bacteria content is 20,000,000,000/gram streptococcus lactis bacterium liquid, viable bacteria content is 20,000,000,000/gram Pediococcus acidilactici bacterium liquid, viable bacteria content is 20,000,000,000/gram Lactobacillus plantarum bacterium liquid, viable bacteria content is 20,000,000,000/gram lactobacillus bulgaricus bacterium liquid and viable bacteria content be 20,000,000,000/gram Bifidobacterium bacterium liquid.
Embodiment 3
(1) by selected for the pleurotus eryngii bacterium chaff after 3 batches of gathering, choose the part that mycelia is pure white, material agllutination is real, nothing is gone mouldy, nothing is rotted, smash, under 150 DEG C of conditions, dry 16 hours with hot air drier, obtain dry bacterium slag, dry for gained bacterium slag is cooled to room temperature, use horizontal continuous stirring micronizer to pulverize, the bacterium chaff powder after pulverizing crosses 300 mesh sieves, obtains bacterium chaff powder;
(2) take 100 kilograms, the bacterium chaff powder of step (1), add 30-40 DEG C, concentration be 60% ethanol 1000 kilograms, soak after 3 hours, then be heated to 50 DEG C of refluxing extraction 36 hours;
(3) the material packaging type squeezer after step (2) refluxing extraction is squeezed, respectively squeezing juice and bacterium chaff slag, squeeze the juice and to filter with 400 order industrial filter cloths, filtrate recycling ethanol, obtain extract, for subsequent use;
(4) the bacterium chaff slag in step (3) is added the water of 1000 kilograms, be heated to 100 DEG C of refluxing extraction 6 hours, then squeeze with packaging type squeezer, obtain squeezing juice; Bacterium chaff slag is not used as him;
(5) the squeezing juice horizontal spiral centrifuge that step (4) obtains is carried out centrifugation, centrifugal rotational speed 9000rpm, obtains supernatant;
(6) supernatant that extract step (3) obtained and step (5) obtain merges, and under temperature 65 DEG C, vacuum-0.085MPa condition, being concentrated into proportion with vacuum rotary evaporator is 1.15, obtains concentrate;
(7) by the concentrate adjusted to ph to 7.0 that step (6) obtains, in 0.7kg/cm
2, sterilizing 35min under 115 DEG C of conditions;
(8) by the concentrate after step (7) sterilizing, naturally cool to less than 35 DEG C and take 82 kilograms, add 10 kilograms, brewer's yeast dry powder, bifidobacteria dry powder 8 kilograms of mixing and stirring that embodiment 1 obtains, filling in the dry glass bottle after sterilization treatment, sealing bottleneck, obtains product of the present invention.After testing, crude protein content is 62.3% to this product; Every gram of total viable bacteria content is 3,600,000,000.
Embodiment 4
(1) respectively by gather the glossy ganoderma after 2 batches and white fungus bacterium chaff selected, choose the part that mycelia is pure white, material agllutination is real, nothing is gone mouldy, nothing is rotted, smash, mixing, dries 12 hours with hot air drier, obtains dry bacterium slag under 300 DEG C of conditions, dry for gained bacterium slag is cooled to room temperature, use horizontal continuous stirring micronizer to pulverize, the bacterium chaff powder after pulverizing crosses 250 mesh sieves, obtains bacterium chaff powder;
(2) take 100 kilograms, the Mixed Microbes chaff powder of step (1), add 30-40 DEG C, concentration be 80% ethanol 1500 kilograms, soak after 6 hours, then be heated to 60 DEG C of refluxing extraction 12 hours;
(3) the material packaging type squeezer after step (2) refluxing extraction is squeezed, respectively squeezing juice and bacterium chaff slag, squeeze the juice and to filter with 400 order industrial filter cloths, filtrate recycling ethanol, obtain extract, for subsequent use;
(4) the bacterium chaff slag in step (3) is added the water of 500 kilograms, be heated to 95 DEG C of refluxing extraction 15 hours, then squeeze with packaging type squeezer, obtain squeezing juice; Bacterium chaff slag is not used as him;
(5) the squeezing juice horizontal spiral centrifuge that step (4) obtains is carried out centrifugation, centrifugal rotational speed 11000rpm, obtains supernatant;
(6) supernatant that extract step (3) obtained and step (5) obtain merges, and under temperature 75 DEG C, vacuum-0.065MPa condition, being concentrated into proportion with plate evaporation inspissator is 1.20, obtains concentrate;
(7) by the concentrate adjusted to ph to 6.2 that step (6) obtains, in 0.7kg/cm
2, sterilizing 35min under 115 DEG C of conditions;
(8) by the concentrate after step (7) sterilizing, naturally cool to after below 35 DEG C and take 72 kilograms, add 20 kilograms, brewer's yeast dry powder, streptococcus thermophilus dried powder 4 kilograms and plant lactobacillus powder 4 kilograms of mixing and stirring that embodiment 1 obtains, filling in the dry glass bottle after sterilization treatment, sealing bottleneck, obtains product of the present invention.After testing, crude protein content is 48.6% to this product; Every gram of total viable bacteria content is 5,600,000,000.
Embodiment 5
(1) by the golden mushroom chaff after 3 batches of gathering, through smashing, dry 14 hours under 250 DEG C of conditions with belt-type hot air drier, bacterium slag must be done, dry for gained bacterium slag is cooled to room temperature, use horizontal continuous stirring micronizer to pulverize, bacterium chaff powder after pulverizing crosses 300 mesh sieves, obtains bacterium chaff powder;
(2) take 100 kilograms, the golden mushroom chaff powder of step (1), add 30-40 DEG C, concentration be 75% ethanol 1200 kilograms, soak after 5 hours, then be heated to 55 DEG C of refluxing extraction 24 hours;
(3) the material packaging type squeezer after step (2) refluxing extraction is squeezed, respectively squeezing juice and bacterium chaff slag, squeeze the juice and to filter with 400 order industrial filter cloths, filtrate recycling ethanol, obtain extract, for subsequent use;
(4) the bacterium chaff slag in step (3) is added the water of 800 kilograms, be heated to 98 DEG C of refluxing extraction 10 hours, then squeeze with packaging type squeezer, obtain squeezing juice; Bacterium chaff slag is not used as him;
(5) the squeezing juice horizontal spiral centrifuge that step (4) obtains is carried out centrifugation, centrifugal rotational speed 10000rpm, obtains supernatant;
(6) supernatant that extract step (3) obtained and step (5) obtain merges, and under temperature 70 C, vacuum-0.075MPa condition, being concentrated into proportion with plate evaporation inspissator is 1.20, obtains concentrate;
(7) by the concentrate adjusted to ph to 6.8 that step (6) obtains, in 0.7kg/cm
2, sterilizing 35min under 115 DEG C of conditions;
(8) by the concentrate after step (7) sterilizing, naturally cool to after below 35 DEG C and take 75 kilograms, add 10 kilograms, brewer's yeast dry powder, 3 kilograms, streptococcus lactis dry powder, 3 kilograms, Pediococcus acidilactici dry powder, plant lactobacillus powder 3 kilograms, 3 kilograms, lactobacillus bulgaricus dry powder and bifidobacteria dry powder 3 kilograms of mixing and stirring that embodiment 1 obtains, filling in the dry glass bottle after sterilization treatment, sealing bottleneck, obtains product of the present invention.After testing, crude protein content is 59.4% to this product; Every gram of total viable count is 5,000,000,000.
Embodiment 6
(1) respectively by gather the flat mushroom after 2 batches and coprinus comatus bacterium chaff selected, choose the part that mycelia is pure white, material agllutination is real, nothing is gone mouldy, nothing is rotted, smash, mixing, dries 12 hours with hot air drier, obtains dry bacterium slag under 250 DEG C of conditions, dry for gained bacterium slag is cooled to room temperature, use horizontal continuous stirring micronizer to pulverize, the bacterium chaff powder after pulverizing crosses 250 mesh sieves, obtains bacterium chaff powder;
(2) take the flat mushroom of step (1), 100 kilograms, coprinus comatus Mixed Microbes chaff powder, add 30-40 DEG C, concentration be 80% ethanol 1300 kilograms, soak after 4 hours, then be heated to 50 DEG C of refluxing extraction 36 hours;
(3) the material packaging type squeezer after step (2) refluxing extraction is squeezed, respectively squeezing juice and bacterium chaff slag, squeeze the juice and to filter with 400 order industrial filter cloths, filtrate recycling ethanol, obtain extract, for subsequent use;
(4) the bacterium chaff slag in step (3) is added the water of 1000 kilograms, be heated to 95 DEG C of refluxing extraction 15 hours, then squeeze with packaging type squeezer, obtain squeezing juice; Bacterium chaff slag is not used as him;
(5) the squeezing juice horizontal spiral centrifuge that step (4) obtains is carried out centrifugation, centrifugal rotational speed 9000rpm, obtains supernatant;
(6) supernatant that extract step (3) obtained and step (5) obtain merges, and under temperature 70 C, vacuum-0.075MPa condition, being concentrated into proportion with plate evaporation inspissator is 1.20, obtains concentrate;
(7) by the concentrate adjusted to ph to 6.9 that step (6) obtains, in 0.7kg/cm
2, sterilizing 35min under 115 DEG C of conditions;
(8) by the concentrate after step (7) sterilizing, naturally cool to after below 35 DEG C and take 78 kilograms, add 10 kilograms, brewer's yeast dry powder, streptococcus thermophilus dried powder 6 kilograms and bifidobacteria dry powder 6 kilograms of mixing and stirring that embodiment 1 obtains, filling in the dry glass bottle after sterilization treatment, sealing bottleneck, obtains product of the present invention.After testing, crude protein content is 64.7% to this product; Every gram of total viable bacteria content is 4,400,000,000.
Embodiment 7
(1) the Lenlinus edodes chaff after 3 batches of gathering is got selected, choose the part that mycelia is pure white, material agllutination is real, nothing is gone mouldy, nothing is rotted, smash, dry 12 hours under 270 DEG C of conditions with hot air drier, bacterium slag must be done, dry for gained bacterium slag is cooled to room temperature, use horizontal continuous stirring micronizer to pulverize, bacterium chaff powder after pulverizing crosses 280 mesh sieves, obtains bacterium chaff powder;
(2) take 100 kilograms, the Lenlinus edodes chaff powder of step (1), add 30-40 DEG C, concentration be 75% ethanol 1100 kilograms, soak after 5 hours, then be heated to 52 DEG C of refluxing extraction 33 hours;
(3) the material packaging type squeezer after step (2) refluxing extraction is squeezed, respectively squeezing juice and bacterium chaff slag, squeeze the juice and to filter with 400 order industrial filter cloths, filtrate recycling ethanol, obtain extract, for subsequent use;
(4) the bacterium chaff slag in step (3) is added the water of 1000 kilograms, be heated to 98 DEG C of refluxing extraction 10 hours, then squeeze with packaging type squeezer, obtain squeezing juice; Bacterium chaff slag is not used as him;
(5) the squeezing juice horizontal spiral centrifuge that step (4) obtains is carried out centrifugation, centrifugal rotational speed 10000rpm, obtains supernatant;
(6) supernatant that extract step (3) obtained and step (5) obtain merges, and under temperature 72 DEG C, vacuum-0.07MPa condition, being concentrated into proportion with plate evaporation inspissator is 1.20, obtains concentrate;
(7) by the concentrate adjusted to ph to 6.7 that step (6) obtains, in 0.7kg/cm
2, sterilizing 35min under 115 DEG C of conditions;
(8) by the concentrate after step (7) sterilizing, naturally cool to after below 35 DEG C and take 73 kilograms, add saccharomyces cerevisiae liquid 15 kilograms, streptococcus lactis bacterium liquid 4 kilograms, lactobacillus bulgaricus bacterium liquid 4 kilograms and Bifidobacterium bacterium liquid 4 kilograms of mixing and stirring that embodiment 2 obtains, filling in the dry glass bottle after sterilization treatment, sealing bottleneck, obtains product of the present invention.Crude protein content is 52.2% after testing; Every gram of total viable bacteria content is 5,400,000,000.
Claims (8)
1. a liquid-type biology feed additive, it is characterized in that by percentage by weight be 10-20% brewer's yeast, 8-15% lactic acid bacteria, 72-82% edible fungus bran extract concentrate form, obtain by the following method:
(1) by the edible mushroom asparagus after 2-3 stubble of gathering, pleurotus eryngii, agaricus bisporus, straw mushroom, flat mushroom, mushroom, phoenix-tail mushroom, Pleurotus nebrodensis, Agrocybe chaxingu, true Ji mushroom, meadow dried mushroom, Agaricus bitorqui, Stropharia rugoso-annulata, wizened mushroom, parasol mushroom, Albatrellus dispansus (Lloyd) Canf. & Gilb, black mushroom, HUAZIGU, Lepista sordida, grifola frondosus, Lactrarius hatsudake, Huang Jugu, Huang Liugu, pixie stool, collybia albuminosa, coprinus comatus, T.lobayense Heim, YUHUANGGU, dried mushroom, ferfas, halimasch, cepe, Fistulina hepatica (Schaeff.) Fr, the newborn bacterium of pine, matsutake, dicyclo mushroom, elegant precious mushroom, hickory chick, Russula vinosa, glossy ganoderma, white fungus, black fungus, any one bacterium chaff in Hericium erinaceus or two or more Mixed Microbes chaff are selected, get mycelia pure white, material agllutination is real, without going mouldy, without the part of rotting, smash, drying, pulverize, edible fungus bran powder after pulverizing crosses 250-300 mesh sieve, obtain bacterium chaff powder,
(2) in the bacterium chaff powder of step (1), add 30-40 DEG C, concentration is the ethanol of 60-90%, bacterium chaff and ethanol weight ratio are 1:10-30, soak after 1-6 hour, then are heated to 50-60 DEG C of refluxing extraction 12-36 hour;
(3) squeezed by the material after step (2) refluxing extraction, obtain squeezing juice and bacterium chaff slag, squeeze the juice and to filter with 400 order industrial filter cloths, filtrate recycling ethanol, obtained extract is stand-by;
(4) water bacterium chaff slag in step (3) being added again bacterium chaff powder weight ratio 5-10 times amount is heated to 95-100 DEG C of refluxing extraction 6-15 hour, then squeezes, and obtains squeezing juice;
(5) by the squeezing juice centrifugation that step (4) obtains, centrifugal rotational speed 9000-16000rpm, obtains supernatant;
(6) supernatant that extract step (3) obtained and step (5) obtain merges, 60-90 DEG C, to be concentrated into proportion under-0.05MPa ~-0.1MPa condition be 1.15-1.20, obtain edible fungus bran and extract concentrate;
(7) the concentrate adjustment pH to 6.2-7.0 step (6) obtained, in 0.7kg/cm
2, sterilizing 35min under 115 DEG C of conditions;
(8) concentrate after step (7) sterilizing is naturally cooled to less than 35 DEG C, take the concentrate of formula ratio and the brewer's yeast of formula ratio and lactic acid bacteria mixing and stirring, filling in the vial after sterilization treatment or plastic bottle, sealing, obtains product.
2. liquid-type biology feed additive as claimed in claim 1, it is characterized in that, described lactic acid bacteria (Lactobacillus) is streptococcus thermophilus (Streptococcus thermophilus), streptococcus lactis (Streptococcus acidi lactici), Pediococcus acidilactici (Pediococcus acidilactici), Lactobacillus plantarum (Lactobacillus plantarum), any one or two kinds and above any combination in lactobacillus bulgaricus (Lactobacillus bulgaricus) and Bifidobacterium (Bacillus bifidus).
3. liquid-type biology feed additive as claimed in claim 1, is characterized in that, the oven dry described in described step (1) is the low temperature hot-air seasoning 8-16 hour of employing 150-400 DEG C; Described pulverizing is ultramicro grinding.
4. liquid-type biology feed additive as claimed in claim 3, it is characterized in that, described ultramicro grinding preferably uses horizontal continuous stirring micronizer.
5. liquid-type biology feed additive as claimed in claim 1, it is characterized in that, in described step (2), the weight ratio of preferred bacterium chaff and ethanol is 1:10-15; Preferred concentration of alcohol is 60-80%; Preferred reflux extracting time is 26-36 hour.
6. liquid-type biology feed additive as claimed in claim 1, it is characterized in that, packaging type squeezer is selected in described step (3) and the squeezing described in (4); Described in step (4), preferably horizontal spiral centrifuge is used to squeezing juice centrifugation; Preferred centrifugal rotational speed 9000-11000rpm.
7. liquid-type biology feed additive as claimed in claim 1, is characterized in that, described step (6) preferably concentrated condition is 65-75 DEG C ,-0.065MPa ~-0.085MPa; Preferred use vacuum rotary evaporator or plate evaporation inspissator.
8. the preparation method of the liquid-type biology feed additive as described in claim 1-7.
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| CN201410832515.8A CN104472883A (en) | 2014-12-29 | 2014-12-29 | Liquid biological feed additive |
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| CN201410832515.8A CN104472883A (en) | 2014-12-29 | 2014-12-29 | Liquid biological feed additive |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105267254A (en) * | 2015-11-03 | 2016-01-27 | 广西南宁胜祺安科技开发有限公司 | Method of extracting Collybia albuminosa polysaccharide from Collybia albuminosa |
| CN107494920A (en) * | 2017-10-12 | 2017-12-22 | 中国林业科学研究院林产化学工业研究所 | It is a kind of using feed with paper-mulberry leaf and edible fungus bran as biological fermentation feed of primary raw material and preparation method thereof |
| CN108094733A (en) * | 2018-01-15 | 2018-06-01 | 魏浩峰 | It is a kind of to prepare the method for livestock feed additive and livestock feed additive by raw material of mushroom bran |
| CN110004191A (en) * | 2019-02-21 | 2019-07-12 | 广州博采生物科技有限公司 | A kind of preparation method of amino acid concentrate and a kind of contain amino acid feed |
| CN110326805A (en) * | 2019-08-15 | 2019-10-15 | 青岛英赛特生物科技有限公司 | A kind of feed fermentation device and feed production method |
| CN112825967A (en) * | 2021-01-18 | 2021-05-25 | 海南金港生物技术股份有限公司 | Formula and preparation method of feed for monkey breeding |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105267254A (en) * | 2015-11-03 | 2016-01-27 | 广西南宁胜祺安科技开发有限公司 | Method of extracting Collybia albuminosa polysaccharide from Collybia albuminosa |
| CN107494920A (en) * | 2017-10-12 | 2017-12-22 | 中国林业科学研究院林产化学工业研究所 | It is a kind of using feed with paper-mulberry leaf and edible fungus bran as biological fermentation feed of primary raw material and preparation method thereof |
| CN108094733A (en) * | 2018-01-15 | 2018-06-01 | 魏浩峰 | It is a kind of to prepare the method for livestock feed additive and livestock feed additive by raw material of mushroom bran |
| CN110004191A (en) * | 2019-02-21 | 2019-07-12 | 广州博采生物科技有限公司 | A kind of preparation method of amino acid concentrate and a kind of contain amino acid feed |
| CN110326805A (en) * | 2019-08-15 | 2019-10-15 | 青岛英赛特生物科技有限公司 | A kind of feed fermentation device and feed production method |
| CN112825967A (en) * | 2021-01-18 | 2021-05-25 | 海南金港生物技术股份有限公司 | Formula and preparation method of feed for monkey breeding |
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