CN104472859A - Biologically fermented fungus bran protein feed additive - Google Patents

Biologically fermented fungus bran protein feed additive Download PDF

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Publication number
CN104472859A
CN104472859A CN201410847667.5A CN201410847667A CN104472859A CN 104472859 A CN104472859 A CN 104472859A CN 201410847667 A CN201410847667 A CN 201410847667A CN 104472859 A CN104472859 A CN 104472859A
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bacterium chaff
additive
protein feed
biofermentation
mushroom
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张奎昌
张志年
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Jiangsu Qianyaotang Traditional Chinese Medicine Research Institute Co Ltd
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Jiangsu Qianyaotang Traditional Chinese Medicine Research Institute Co Ltd
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Abstract

The invention discloses a biologically fermented fungus bran protein feed additive. The invention adopts the technical scheme that the biologically fermented fungus bran protein feed additive is prepared by the following steps: drying and crushing edible and medicinal fungus bran, heating, carrying out reflux extraction, concentrating to prepare a concentrated solution, and then utilizing 88-92% of the fungus bran extraction concentrated solution and 8-12% of a compound bacteria leavening agent, carrying out culture and fermentation for 48-100 hours according to the conditions that the temperature is 25-35 DEG C, the stirring rate is 45-120 r/min, and the ventilator capacity is 1: (0.5-1.5) v/v per minute, and at last carrying out spray drying to obtain the product. The biologically fermented fungus bran protein feed additive obtained by the method disclosed by the invention has the crude protein content of 42-48%, so that the nutrition of the additive can be greatly promoted, the plant protein grain resources can be reduced, the waste fungus bran is turned to be useful, and the economic benefit of the industrial chain from edible and medicinal fungus culture to biologic protein feed processing to livestock healthy breeding can be realized; the method is simple in process, low in production cost, obvious in economic benefit, and good in application prospect.

Description

A kind of biofermentation bacterium chaff additive of protein feed
Technical field
The present invention relates to the processing and utilization of edible and medical fungi bacterium chaff, relate to feed addictive manufacture field simultaneously, be specifically related to a kind of biofermentation bacterium chaff additive of protein feed.
Background technology
Bacterium chaff utilizes wood chip, cotton seed hulls, corncob, wheat bran, rice husk, waste cotton, alditol slag, xylose residue, bagasse, megasse, vinasse, rape cake and multiple kinds of crops powder of straw to carry out edible and medical fungi substituting stuff cultivation for primary raw material, culture medium residue after results, is commonly called as edible and medical fungi cultivation waste material, bacterium slag or clout; It is the compound of the compositions such as the crude fibre containing the residual body of edible and medical fungi mycelia and edible and medical fungi enzymolysis structure generation qualitative change.
China's edible and medical fungi cultivation industry development is rapid, and industry size is huge, is edible and medical fungi big producing country of the world, is also edible and medical fungi big export country, only edible mushroom annual production about 1,100 ten thousand tons in the world.Edible and medical fungi remains cultivation base stock-i.e. bacterium chaff postpartum, and about have 20% to be converted into fructification in every hundred kilograms of siccatives, 20% comparatively becomes thermal energy consumption, and its quantity is edible and medical fungi compost 60%, is about 6,000,000 tons.Bacterium chaff has mycelium to grow in a large number, nutritive value is higher, analyze according to there being scholar, in edible fungus bran, gross protein value is about 2%-12%, crude fat average out to 0.8%-1.2%, crude fibre is on average about 14%-23%, simultaneously also containing abundant mineral matter element and the trace element such as calcium, phosphorus, zinc, copper, iron, magnesium.Medicinal fungus is as Ganoderma Lucidum sugar, and through Ganoderma Lucidum degradation, crude fibre is degraded to 23.0% from 34.5% of Medium for Ganoderma lucidum material, reduces 11.5%, and crude protein is increased to 10.2% from 5.0%, increases by 5.2%; Crude fat is increased to 4.7% from 0.40%; Crude fibre significantly reduces, and nutritive value increases substantially, dry gross energy 3.38 thermie/kilogram.For pig, digestible energy can reach 2.54 thermies/kilogram, close with the digestible energy of wheat skin, be equivalent to corn digestible energy 75%, but crude protein content is higher than corn and Mai Pi.Along with China's sustained and rapid development, national life level and quality constantly improve, and this just requires that aquaculture provides how better animal protein, to meet the living needs improved constantly.The basis of aquaculture is feed, and the primary raw material of feed derives from corn.To the demand that meat increases, need extensive development livestock breeding industry, need a large amount of feeding grain, this has also just increased the weight of the pressure of food supply.
The output of China's corn is in recent years about 1.3 hundred million tons, wherein 40% be used as feed, and the year growth rate of demand of fodder maize is 4%, and therefore, China every year also will from a large amount of corn of foreign procurement to solve the problem of feedstuff deficiency.If the bacterium chaff being about 6,000,000 tons every year can be developed as feed, be just equivalent to the corn producing more than 400 ten thousand tons every year more, to the not enough problem of solution China feedstuff, there is certain realistic meaning.
Bacterium chaff fermented feed is the feed new product that environment-friendly type turns waste into wealth, be rich in protein, amino acid, calcium, the mineral matters such as phosphorus, can be used as basal feed raw material, add appropriate amount of auxiliary materials and carry out nutrient balance, for configuring the functional feed of different purposes, also alternative Partial Fish Meal, the fine fodders such as dregs of beans, to reduce costs, owing to containing useful thalline and metabolite thereof in bacterium chaff fermented feed, not only good palatability, and the breeding of animal intestinal multiple beneficial bacterium can be promoted, suppress pathogen, improve immunologic function, and various digestive ferment can be secreted by stimulating animal, strengthen the appetite of animal, improve digestibility, thus promote growing of animal.
According to edible and medical fungi bacterium chaff contain fiber more, decompose not exclusively, many deficiency factors such as palatability is poor, nutritional utilization is insufficient, with regard to how to obtain, feed nutrient is high, fiber hydrolization is more complete, the nutrient composition content such as protein, fat is high, and edible and medical fungi bacterium chaff being obtained efficiently, fully utilizes fully is the important topic that will solve at present.
Existing Chinese patent (CN1810144A) specifically discloses the processing method preparing additive of protein feed with waste mushroom leftover, its technical scheme utilizes the method for steam flash that the bacterium block of crushing is transformed, remove portion unwanted metabolic products and toxin, and kill all contaminating microorganisms that raw material adheres to, allow steam outside explosion type in raw material spray simultaneously, the undecomposed cellulose of raw material etc. is made to expand with cracked, to increase cellulosic surface area, 21.58-58.25 part auxiliary material is added and 30-50 part water is mixed thoroughly in 100 parts of bacterium blocks after flash distillation, and inoculate 5-10 part saccharomycete and 5-10 part wood is mould, 2-3 days is cultivated at 28-32 DEG C.Inorganic nitrogen in bacterium block is converted into high-quality protein by object, produce the nutrients such as abundant vitamin, and can greatly improve palatability and taste, in the product that this invention obtains, crude protein content is 20%, also be only create materials such as bacterium chaff celluloses to expand and cracked etc. with regard to utilizing steam flash in this scheme of the invention, be only be beneficial to saccharomycete and the mould growth of wood, but not cellulose can be made to remove, a large amount of fibers being difficult to digest and assimilate directly affects the quality problems of additive of protein feed.
Summary of the invention
The present invention be directed to the deficiencies in the prior art, a kind of biofermentation bacterium chaff additive of protein feed is provided, the solution of the present invention is by bacterium chaff drying, pulverize, add water refluxing extraction, concentrated obtained concentrate, then the concentrate of weight 88%-92% and the compound agglomeration of weight 8%-12% are passed through cultivation and fermentation, spray-dried obtained product of the present invention again, a large amount of fibers in bacterium chaff are removed by the program, extract a large amount of water-soluble finished product contained in protein in its mycelium and bacterium chaff, object improves to extract protein component in bacterium chaff and other useful nutrients, remove the cellulose components being difficult in bacterium chaff digest and assimilate, the organic principle extracted can be made further to obtain further biological conversion by the fermentation of compound agglomeration, promote mycoprotein content, increase palatability, improve absorption rate, promote the efficiency utilization of bacterium chaff Protein processing, promote the effect of bacterium chaff processing additive of protein feed.
In order to realize the present invention, specifically have employed following technical scheme:
A kind of biofermentation bacterium chaff additive of protein feed, it is characterized in that, obtained by the following method by the bacterium chaff lixiviate concentrate of percentage by weight 88%-92% and the compound agglomeration of 8%-12%: (1) gets bacterium chaff drying, pulverizing, cross 200-300 mesh sieve after pulverizing, obtain bacterium chaff powder; (2) bacterium chaff powder is added 40-45 DEG C of water, the weight ratio of bacterium chaff and water is 1:8-10, soaks 3-6 hour; (3) by the heating material refluxing extraction 8-12 hour after immersion, the temperature of heating and refluxing extraction is 90-95 DEG C; (4) material after heating and refluxing extraction is squeezed, obtain squeezing juice and bacterium chaff slag; (5) bacterium chaff slag is added again the water of bacterium chaff weight ratio 5 times, heating and refluxing extraction 3-5 hour, the temperature of heating and refluxing extraction is 95-100 DEG C, then squeezes, obtains squeezing juice; Bacterium chaff slag separately uses it for anything else; (6) squeezing juice (4) and (5) obtained merges centrifugation, and centrifugal rotational speed 9000-16000rpm, obtains supernatant; (7) supernatant obtained is filtered, obtain filtrate; (8) by filtrate 60-90 DEG C, to be concentrated into proportion under 0.055MPa-0.1MPa condition be 1.15-1.25/40 DEG C of survey, obtains concentrate; (9) concentrate is placed in fermentation tank, when being cooled to below 35 DEG C, add the compound agglomeration of formula ratio, 25-35 DEG C, mixing speed 45-120r/min, throughput 1:0.5-1.5V/V/min condition under cultivation and fermentation 48-100 hour, obtain zymotic fluid; (10) by fermentation liquor spraying dry, make moisture below 6%, obtain finished product.
According to the present invention, do to optimize further and/or select to such scheme.
Bacterium chaff described above is utilize the single raw material of boll hull, peanut shell, rice husk, wood chip, corncob, stalk or mixed material to carry out edible and medical fungi substituting stuff cultivation, the culture medium bacterium slag after results edible and medical fungi, edible and medical fungi pleurotus eryngii can be adopted, asparagus, white fungus, black fungus, glossy ganoderma, agaricus bisporus, straw mushroom, flat mushroom, mushroom, Pleurotus nebrodensis, Agrocybe chaxingu, true Ji mushroom, meadow dried mushroom, Agaricus bitorqui, Stropharia rugoso-annulata, wizened mushroom, phoenix-tail mushroom, parasol mushroom, Albatrellus dispansus (Lloyd) Canf. & Gilb, black mushroom, Hericium erinaceus, HUAZIGU, Lepista sordida, grifola frondosus, Lactrarius hatsudake, Huang Jugu, Huang Liugu, pixie stool, collybia albuminosa, coprinus comatus, T.lobayense Heim, YUHUANGGU, Pleurotus citrinopileatus, dried mushroom, ferfas, halimasch, cepe, Fistulina hepatica (Schaeff.) Fr, the newborn bacterium of pine, matsutake, dicyclo mushroom, elegant precious mushroom, hickory chick, a kind of bacterium chaff in Russula vinosa or the mixture of multiple bacterium chaff.
Compound agglomeration described above, can select to adopt streptococcus thermophilus (Streptococcus thermophilus) to be 1:1.5:1.5 proportions by weight with bacillus subtilis (Bacillus subtilis) and saccharomycete (Saccharomyces).
Compound agglomeration described above, can select to adopt addicted to yogurt coccus (Lactobacillus lactis medicine) and bacillus licheniformis (Bacillus licheniform is) and saccharomycete (Saccharomyces) is 1:1.5:1.5 proportions by weight.
Compound agglomeration described above, can also select to adopt lactobacillus acidophilus (Leatobacillus acidophilus) to be 1:1.5:1.5 proportions by weight with Bacillus circulans (Bacillus circulans) and saccharomycete (Saccharomyces).
Above-mentioned compound agglomeration, can also select to adopt Bifidobacterium (Bacillus bifidus) to be 1:1.5:1.5 proportions by weight with bacillus firmus (Bacillus firmus) and saccharomycete (Saccharomyces).
Saccharomycete (Saccharomyces) described above can any one in choice for use candida utili (Candida utilis), brewer's yeast (Saccharomyces cerevisiae), brewer's yeast (Saccharomyces cerevisiae) and fodder yeast (Candida Tropicali); Preferred use fodder yeast (Candida Tropicali).
Streptococcus thermophilus described in compound agglomeration described above, choice for use is commercially available respectively all feed addictive every gram viable bacteria concentration that are used as can be no less than the dry powder goods of 20,000,000,000 addicted to yogurt coccus, lactobacillus acidophilus, Bifidobacterium, bacillus subtilis, bacillus licheniformis, Bacillus circulans and bacillus firmus.
Described candida utili, brewer's yeast, brewer's yeast and fodder yeast in compound agglomeration described above can select every gram of viable bacteria concentration commercially available to be no less than the dry powder goods of 20,000,000,000.
Described above every gram is no less than to the streptococcus thermophilus of 20,000,000,000 containing viable bacteria concentration, addicted to yogurt coccus, lactobacillus acidophilus, Bifidobacterium, bacillus subtilis, bacillus licheniformis, Bacillus circulans, bacillus firmus and candida utili, brewer's yeast, the thalline dry powder goods of brewer's yeast and fodder yeast, except selecting viable bacteria dry powder goods commercially available, also can cultivate under applicable condition according to existing conventional method individually according to each bacterial classification, can find from relevant handbook as condition of culture (as temperature etc.), as handbook or the textbook of the cultivation about microorganism, in order to be applicable to the fermentation process to material of the present invention, Liquid Culture Zymolysis Equipment is adopted to cultivate respectively in respective independently cultivating system by the culture medium optimized, then with high speed centrifugation, the thalline in zymotic fluid is separated, be placed in dry indoor cold air drying or by vacuum freeze drying mode, the drying of bacterium mud obtained, the viable bacteria concentration of the thalline dry powder obtained respectively is 20,000,000,000 every gram.Prior art is utilized to cultivate the viable bacteria dry powder goods obtained, effectively can preserve with easy to use to goods at excellent storage environment, but, in the present invention also according to produce temporary needs but not when need preserve, also the bacterium mud that zymotic fluid adopts high speed centrifugation to separate by said method is adopted, be diluted in every ml with sterilized water and contain the flowing product that viable bacteria is 20,000,000,000, also obtain same result of use.
Oven dry described in above-mentioned steps (1) is the low temperature drying 12-16 hour of employing 100-300 DEG C.
Oven dry described in above-mentioned steps (1) adopts hot-air seasoning or far infrared drying, and described pulverizing is ultramicro grinding; Preferred use vibration superfine disintegrator.
Packaging type squeezer is selected in above-mentioned steps (4) and the squeezing described in (5); Squeezing juice centrifugation described in step (6), preferably uses horizontal spiral centrifuge, preferred centrifugal rotational speed 9000-10000rpm; Filtration described in step (7) uses diatomite filtration.
In above-mentioned steps (8), preferred concentrated condition is 70-80 DEG C ,-0.065MPa ~-0.085MPa.
Concentrated described in above-mentioned steps (8) selects vacuum rotary evaporator or tubular type evaporation concentrator.
In above-mentioned steps (9), preferred cultivation and fermentation condition is at 29-33 DEG C, mixing speed 45-70r/min, throughput 1:0.5-0.7V/V/min cultivation and fermentation 48-72 hour.
The above-described equipment of the present invention is this area conventional equipment.
Do not state the operation of its technical process in processing step in the present invention in detail, be the routine operation in the general knowledge of those skilled in the art.
Except as otherwise noted, percentage of the present invention is all weight percentage.
Beneficial effect of the present invention:
(1) because the present invention takes to carry out ultramicro grinding by after the oven dry of bacterium chaff, make the hyphal cell wall of bacterium chaff damaged, soaking a period of time is beneficial to nutritional labeling stripping, a large amount of mycoprotein, amino acid, carbohydrate is made to obtain fully or completely extracting in refluxing extraction process, simultaneously by cellulose components after ultramicro grinding, also effectively nutriment contained in cellulose effectively can be extracted, be promoted the trophic function of extract.
(2) the present invention makes the nutrition in bacterium chaff be extracted to greatest extent owing to bacterium chaff Ultramicro-powder to be carried out secondary back extraction, and the cellulose composition of indissoluble is given filtering, its extract is through concentrated, compound bacteria-fermented, make the protein in product, the nutritions such as amino acid more directly adopt the protein that bacterium chaff ferments to significantly improve, the biofermentation bacterium chaff protein feed protein content that the inventive method obtains significantly improves, the biofermentation bacterium chaff additive of protein feed crude protein content that the inventive method obtains is 42-48%, 22%-28% is improve containing crude protein 20% than in prior art.
(3) owing to present invention employs compound bacteria-fermented technique, product is made to produce multiple mycoprotein, cell protein, digestive ferment during the fermentation, not only significant increase trophism, also improve palatability and attractant greatly simultaneously, because the present invention adopts Effect of Alcohol Extracting Procedureto Total, make manufactured goods good water solubility, be beneficial to absorption and the utilization of animal, enhance the result of use of product.
(4) utilize biofermentation bacterium chaff additive of protein feed 25% of the present invention to substitute 20% dregs of beans to add 80 days in the pig feed of more than 25kg, average daily gain is up to 837.5g, and comparatively control group improves 26.4%, and feed conversion rate improves 16.6%; As 90kg delivers for sale, on average 12.6 days can be shifted to an earlier date.
Utilize biofermentation bacterium chaff additive of protein feed of the present invention 8% substitute 5% dregs of beans add within 60th, deliver for sale fatten in rabbit daily ration, rate of body weight gain and the price of deed can be significantly improved, reduce feedstuff-meat ratio, obtain good economic benefit, daily gain, the price of deed, overall economic efficiency improve 17.4%, 13.9% and 22.5% respectively.
Biofermentation bacterium chaff additive of protein feed 10% of the present invention is utilized to add 1 month in daily ration of broiler, test group weightening finish 545g, and control group weightening finish 468g; Test group efficiency of feed utilization improves 4%-5%.
(5) the inventive method can make full use of bacterium chaff extract mycoprotein prepare feed addictive, a large amount of bacterium chaffs not only can be made to be recycled utilization, and vegetable protein grain resource can be saved, this waste resource of bacterium chaff is turned waste into wealth, the bacterium chaff slag simultaneously extracted after bacterium chaff is again the good resource preparing organic fertilizer, make bacterium chaff can obtain effectively utilizing completely, can effectively promote edible bacterium to grow, the overall economic efficiency of feed addictive processing and fertilizer production, and the inventive method technique is simple, production cost is low, remarkable in economical benefits, there is good implementation prospect.
Detailed description of the invention
Following examples are only further illustrate of the invention process, and should not be considered as limitation of the present invention.
Embodiment 1
(1) get edible mushroom flat mushroom boll hull bacterium chaff, smash, under the condition of 300 DEG C, dry 12 hours with hot air drier, obtain the dry slag of bacterium chaff, dry slag is cooled to room temperature, use vibration superfine disintegrator to pulverize, the bacterium chaff powder of pulverizing crosses 300 mesh sieves, obtains bacterium chaff powder; (2) take 100 kilograms, bacterium chaff powder, add the hot water 1000 kilograms of 40-45 DEG C, soak 3 hours; (3) by heating material to the 95 DEG C refluxing extraction 8 hours after soaking; (4) the material packaging type squeezer after heating and refluxing extraction is squeezed, obtain squeezing juice and bacterium chaff slag; (5) bacterium chaff slag is added the water of 500 kilograms again, be heated to 100 DEG C of refluxing extraction 3 hours, then adopt packaging type squeezer to squeeze, obtain squeezing juice; (6) squeezing juice (4) and (5) obtained respectively merges, and use horizontal centrifuge centrifugation, centrifugal rotational speed 9000rpm, obtains supernatant; (7) supernatant obtained is added 10 kilograms of diatomite, filter with diatomite filter, obtain filtrate; (8) by filtrate vacuum rotary evaporator temperature be 70 DEG C, vacuum for concentrated under-0.085Mpa condition, being concentrated into proportion is 1.15/40 DEG C of survey, obtains concentrate; (9) getting concentrate 88 kilograms is placed in fermentation tank, to be cooled to less than 35 DEG C time, add by streptococcus thermophilus 3 kilograms, bacillus subtilis 4.5 kilograms and fodder yeast bacterium 4.5 kilograms of compound agglomeration formed, under temperature 33 DEG C, mixing speed 70r/min, throughput 1:0.7V/V/min condition, cultivation and fermentation 48 hours, obtains zymotic fluid; (10) by fermentation liquor spraying dry, make moisture below 6%, get product.Product coarse protein content is 48.3%.
Embodiment 2
(1) get edible mushroom pleurotus eryngii wood chip bacterium chaff, smash, dry 14 hours, obtain the dry slag of bacterium chaff, dry slag is cooled to room temperature under the condition of 200 DEG C with hot air conveying type drying box, use vibration superfine disintegrator to pulverize, the bacterium chaff powder of pulverizing crosses 200 mesh sieves, obtains bacterium chaff powder; (2) take 100 kilograms, bacterium chaff powder, add the hot water 800 kilograms of 40-45 DEG C, soak 6 hours; (3) by heating material to the 90 DEG C refluxing extraction 12 hours after soaking; (4) the material packaging type squeezer after heating and refluxing extraction is squeezed, obtain squeezing juice and bacterium chaff slag; (5) bacterium chaff slag is added the water of 500 kilograms again, be heated to 95 DEG C of refluxing extraction 5 hours, then adopt packaging type squeezer to squeeze, obtain squeezing juice; (6) squeezing juice (4) and (5) obtained respectively merges, and use horizontal centrifuge centrifugation, centrifugal rotational speed 10000rpm, obtains supernatant; (7) supernatant obtained is added 8 kilograms of diatomite, filter with diatomite filter, obtain filtrate; (8) by filtrate vacuum rotary evaporator temperature be 80 DEG C, vacuum for concentrated under-0.065Mpa condition, being concentrated into proportion is 1.25/40 DEG C of survey, obtains concentrate; (9) getting concentrate 92 kilograms is placed in fermentation tank, to be cooled to less than 35 DEG C time, add by streptococcus thermophilus 2 kilograms, bacillus licheniformis 3 kilograms and candida utili 3 kilograms of compound agglomeration formed, under temperature 30 DEG C, mixing speed 50r/min, throughput 1:0.5V/V/min condition, cultivation and fermentation 50 hours, obtains zymotic fluid; (10) by fermentation liquor spraying dry, make moisture below 6%, get product.Product coarse protein content is 46.2%.
Embodiment 3
(1) take food and smash mixing respectively with Ganoderma wood chip bacterium chaff and white fungus corncob bacterium chaff, under the condition of 150 DEG C, dry 15 hours with hot air conveying type drying box, obtain the dry slag of bacterium chaff, dry slag is cooled to room temperature, use vibration superfine disintegrator is pulverized, and the bacterium chaff powder of pulverizing crosses 250 mesh sieves, obtains bacterium chaff powder; (2) take Mixed Microbes chaff 100 kilograms, add the hot water 900 kilograms of 40-45 DEG C, soak 5 hours; (3) by heating material to the 95 DEG C refluxing extraction 8 hours after soaking; (4) the material packaging type squeezer after heating and refluxing extraction is squeezed, obtain squeezing juice and bacterium chaff slag; (5) bacterium chaff slag is added the water of 500 kilograms again, be heated to 95 DEG C of refluxing extraction 5 hours, then adopt packaging type squeezer to squeeze, obtain squeezing juice; (6) squeezing juice (4) and (5) obtained respectively merges, and use horizontal centrifuge centrifugation, centrifugal rotational speed 9500rpm, obtains supernatant; (7) supernatant obtained is added 9 kilograms of diatomite, filter with diatomite filter, obtain filtrate; (8) by filtrate vacuum rotary evaporator temperature be 75 DEG C, vacuum for concentrated under-0.075Mpa condition, being concentrated into proportion is 1.20/40 DEG C of survey, obtains concentrate; (9) getting concentrate 90 kilograms is placed in fermentation tank, to be cooled to less than 35 DEG C time, add by lactobacillus acidophilus 2.5 kilograms, Bacillus circulans 3.75 kilograms and fodder yeast 3.75 kilograms of compound agglomeration formed, under temperature 32 DEG C, mixing speed 60r/min, throughput 1:0.6V/V/min condition, cultivation and fermentation 49 hours, obtains zymotic fluid; (10) by fermentation liquor spraying dry, make moisture below 6%, get product.Product coarse protein content is 42.8%.
According to the present invention, every gram when being no less than the bacterial classification dry powder goods of 20,000,000,000 containing viable bacteria concentration required for the present invention can not be met in market, existing method also can be adopted to obtain.
Embodiment 4
Use corresponding Optimal Medium, Liquid Culture Zymolysis Equipment is adopted individually to cultivate Bifidobacterium, bacillus firmus and fodder yeast bacterium, then with supercentrifuge, the thalline in zymotic fluid is separated, by vacuum freeze drying mode, centrifugation bacterium mud out is dry, obtain respectively viable bacteria content be 20,000,000,000/gram bifidobacteria dry powder, viable bacteria content to be the bacillus firmus dry powder of 20,000,000,000/gram and viable bacteria content the be feed yeast powder of 20,000,000,000/gram.
Embodiment 5
(1) get edible mushroom mushroom wood chip bacterium chaff, smash and dry 16 hours with hot air conveying type drying box under the condition of 100 DEG C, obtain the dry slag of bacterium chaff, dry slag is cooled to room temperature, use vibration superfine disintegrator to pulverize, the bacterium chaff powder of pulverizing crosses 200 mesh sieves, obtains bacterium chaff powder; (2) take 100 kilograms, bacterium chaff powder, add the hot water 900 kilograms of 40-45 DEG C, soak 5 hours; (3) by heating material to the 90 DEG C refluxing extraction 10 hours after soaking; (4) the material packaging type squeezer after heating and refluxing extraction is squeezed, obtain squeezing juice and bacterium chaff slag; (5) bacterium chaff slag is added the water of 500 kilograms again, be heated to 100 DEG C of refluxing extraction 3 hours, then adopt packaging type squeezer to squeeze, obtain squeezing juice; (6) squeezing juice (4) and (5) obtained respectively merges, and use horizontal centrifuge centrifugation, centrifugal rotational speed 10000rpm, obtains supernatant; (7) supernatant obtained is added 7 kilograms of diatomite, filter with diatomite filter, obtain filtrate; (8) by filtrate vacuum rotary evaporator temperature be 78 DEG C, vacuum for concentrated under-0.07Mpa condition, being concentrated into proportion is 1.20/40 DEG C of survey, obtains concentrate; (9) getting concentrate 91 kilograms is placed in fermentation tank, to be cooled to less than 35 DEG C time, add bifidobacteria dry powder 2.25 kilograms that embodiment 4 obtains, the compound agglomeration of bacillus firmus 3.375 kilograms that embodiment 4 obtains and the fodder yeast 3.375 kilograms composition that embodiment 4 obtains, under temperature 29 DEG C, mixing speed 45r/min, throughput 1:0.5V/V/min condition, cultivation and fermentation 72 hours, obtains zymotic fluid; (10) by fermentation liquor spraying dry, make moisture below 6%, get product.Product coarse protein content is 47.8%.

Claims (18)

1. a biofermentation bacterium chaff additive of protein feed, it is characterized in that, obtained by the following method by the bacterium chaff lixiviate concentrate of percentage by weight 88%-92% and the compound agglomeration of 8%-12%: (1) gets bacterium chaff drying, pulverizing, cross 200-300 mesh sieve after pulverizing, obtain bacterium chaff powder; (2) bacterium chaff powder is added 40-45 DEG C of water, the weight ratio of bacterium chaff and water is 1:8-10, soaks 3-6 hour; (3) by the heating material refluxing extraction 8-12 hour after immersion, the temperature of heating and refluxing extraction is 90-95 DEG C; (4) material after heating and refluxing extraction is squeezed, obtain squeezing juice and bacterium chaff slag; (5) bacterium chaff slag is added again the water of bacterium chaff weight ratio 5 times, heating and refluxing extraction 3-5 hour, the temperature of heating and refluxing extraction is 95-100 DEG C, then squeezes, obtains squeezing juice; (6) squeezing juice (4) and (5) obtained merges centrifugation, and centrifugal rotational speed 9000-16000rpm, obtains supernatant; (7) supernatant obtained is filtered, obtain filtrate; (8) by filtrate 60-90 DEG C, to be concentrated into proportion under 0.055MPa-0.1MPa condition be 1.15-1.25/40 DEG C of survey, obtains concentrate; (9) concentrate is placed in fermentation tank, when being cooled to below 35 DEG C, add the compound agglomeration of formula ratio, 25-35 DEG C, mixing speed 45-120r/min, throughput 1:0.5-1.5V/V/min condition under cultivation and fermentation 48-100 hour, obtain zymotic fluid; (10) by fermentation liquor spraying dry, make moisture below 6%, obtain finished product.
2. a kind of biofermentation bacterium chaff additive of protein feed according to claim 1, it is characterized in that, described bacterium chaff adopts edible and medical fungi pleurotus eryngii, asparagus, white fungus, black fungus, glossy ganoderma, agaricus bisporus, straw mushroom, flat mushroom, mushroom, Pleurotus nebrodensis, Agrocybe chaxingu, true Ji mushroom, meadow dried mushroom, Agaricus bitorqui, Stropharia rugoso-annulata, wizened mushroom, phoenix-tail mushroom, parasol mushroom, Albatrellus dispansus (Lloyd) Canf. & Gilb, black mushroom, Hericium erinaceus, HUAZIGU, Lepista sordida, grifola frondosus, Lactrarius hatsudake, Huang Jugu, Huang Liugu, pixie stool, collybia albuminosa, coprinus comatus, T.lobayense Heim, YUHUANGGU, Pleurotus citrinopileatus, dried mushroom, ferfas, halimasch, cepe, Fistulina hepatica (Schaeff.) Fr, the newborn bacterium of pine, matsutake, dicyclo mushroom, elegant precious mushroom, hickory chick, a kind of bacterium chaff in Russula vinosa or the mixture of multiple bacterium chaff.
3. a kind of biofermentation bacterium chaff additive of protein feed according to claim 1, it is characterized in that, described compound agglomeration, streptococcus thermophilus (Streptococcus thermophilus) and bacillus subtilis (Bacillus subtilis) and saccharomycete (Saccharomyces) are 1:1.5:1.5 proportions by weight.
4. a kind of biofermentation bacterium chaff additive of protein feed according to claim 1, it is characterized in that, described compound bacteria is by being 1:1.5:1.5 proportions by weight addicted to yogurt coccus (Lactobacillus lactis medicine) and bacillus licheniformis (Bacillus licheniform is) and saccharomycete (Saccharomyces).
5. a kind of biofermentation bacterium chaff additive of protein feed according to claim 1, it is characterized in that, described compound bacteria is 1:1.5:1.5 proportions by lactobacillus acidophilus (Leatobacillus acidophilus) and Bacillus circulans (Bacillus circulans) and saccharomycete (Saccharomyces) by weight.
6. a kind of biofermentation bacterium chaff additive of protein feed according to claim 1, it is characterized in that, described compound bacteria is 1:1.5:1.5 proportions by Bifidobacterium (Bacillus bifidus) and bacillus firmus (Bacillus firmus) and saccharomycete (Saccharomyces) by weight.
7. a kind of biofermentation bacterium chaff additive of protein feed according to any one of claim 3-6, it is characterized in that, described saccharomycete adopt in candida utili (Candida utilis), brewer's yeast (Saccharomyces cerevisiae), brewer's yeast (Saccharomyces cerevisiae) and fodder yeast (Candida Tropicali) any one.
8. a kind of biofermentation bacterium chaff additive of protein feed as claimed in claim 7, is characterized in that, described saccharomycete adopts fodder yeast.
9. a kind of biofermentation bacterium chaff additive of protein feed according to claim 7 or 8, it is characterized in that, described candida utili, brewer's yeast, brewer's yeast and fodder yeast adopt every gram of viable bacteria concentration commercially available to be no less than the dry powder goods of 20,000,000,000.
10. a kind of biofermentation bacterium chaff additive of protein feed according to any one of claim 3-6, it is characterized in that, described streptococcus thermophilus, the dry powder goods adopting every gram of viable bacteria content commercially available to be 20,000,000,000 addicted to yogurt coccus, lactobacillus acidophilus, Bifidobacterium, bacillus subtilis, bacillus licheniformis, Bacillus circulans, bacillus firmus respectively.
11. a kind of biofermentation bacterium chaff additive of protein feed according to claim 1, is characterized in that, the oven dry described in described step (1), adopt the low temperature drying 12-16 hour of 100-300 DEG C; Described oven dry adopts hot-air seasoning or far infrared drying; Described pulverizing is ultramicro grinding.
12. a kind of biofermentation bacterium chaff additive of protein feed as claimed in claim 11, is characterized in that, described ultramicro grinding uses vibration superfine disintegrator.
13. a kind of biofermentation bacterium chaff additive of protein feed according to claim 1, it is characterized in that, packaging type squeezer is selected in described step (4) and the squeezing described in step (5).
14. a kind of biofermentation bacterium chaff additive of protein feed according to claim 1, it is characterized in that, the squeezing juice centrifugation described in described step (6), preferably uses horizontal spiral centrifuge; Preferred centrifugal rotational speed 9000-10000rpm; Filtration described in described step (7) uses diatomite filtration.
15. a kind of biofermentation bacterium chaff additive of protein feed according to claim 1, is characterized in that, in described step (8), preferred concentrated condition is 70-80 DEG C ,-0.065MPa ~-0.085Mpa; Preferred use vacuum rotary evaporator or tubular type evaporation concentrator.
16. a kind of biofermentation bacterium chaff additive of protein feed according to claim 1, it is characterized in that, in described step (9) preferred cultivation and fermentation condition be 29-33 DEG C, mixing speed 45-70r/min, throughput 1:0.5-0.7V/V/min, cultivation and fermentation 48-72 hour.
17. a kind of biofermentation bacterium chaff additive of protein feed according to claim 1, it is characterized in that, the crude protein content of described biofermentation bacterium chaff additive of protein feed is 42-48W/W%.
The preparation method of 18. 1 kinds of biofermentation bacterium chaff additive of protein feed as described in any one of claim 1-16.
CN201410847667.5A 2014-12-29 2014-12-29 Biologically fermented fungus bran protein feed additive Pending CN104472859A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104938784A (en) * 2015-06-26 2015-09-30 绥化学院 Method for preparing animal probiotics fodder by fermenting edible mushroom waste, straw and pasture
CN106359901A (en) * 2016-08-29 2017-02-01 青岛润达生物科技有限公司 Compound product of glossy ganoderma polysaccharide and lactic acid bacteria
CN107494928A (en) * 2017-09-19 2017-12-22 北京化工大学 A kind of method of composite bacteria solid state fermentation mushroom bran production protein feed
CN108522878A (en) * 2018-01-15 2018-09-14 魏浩峰 A method of preparing poultry fodder additive coproduction organic fungi-manure by raw material of mushroom bran
CN108913627A (en) * 2018-07-27 2018-11-30 青岛农业大学 A kind of leavening of aflatoxin degradation, preparation method and application

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104938784A (en) * 2015-06-26 2015-09-30 绥化学院 Method for preparing animal probiotics fodder by fermenting edible mushroom waste, straw and pasture
CN104938784B (en) * 2015-06-26 2018-05-11 绥化学院 The method for preparing animal probiotics feed by utilizing with waste material of edible mushroom stalk and herbage fermentation
CN106359901A (en) * 2016-08-29 2017-02-01 青岛润达生物科技有限公司 Compound product of glossy ganoderma polysaccharide and lactic acid bacteria
CN107494928A (en) * 2017-09-19 2017-12-22 北京化工大学 A kind of method of composite bacteria solid state fermentation mushroom bran production protein feed
CN108522878A (en) * 2018-01-15 2018-09-14 魏浩峰 A method of preparing poultry fodder additive coproduction organic fungi-manure by raw material of mushroom bran
CN108913627A (en) * 2018-07-27 2018-11-30 青岛农业大学 A kind of leavening of aflatoxin degradation, preparation method and application
CN108913627B (en) * 2018-07-27 2019-08-30 青岛农业大学 A kind of leavening of aflatoxin degradation, preparation method and application

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Application publication date: 20150401