CN104489271A - Health nutritional liquid for animals - Google Patents
Health nutritional liquid for animals Download PDFInfo
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- CN104489271A CN104489271A CN201410826813.6A CN201410826813A CN104489271A CN 104489271 A CN104489271 A CN 104489271A CN 201410826813 A CN201410826813 A CN 201410826813A CN 104489271 A CN104489271 A CN 104489271A
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Abstract
The invention discloses a health nutritional liquid for animals. The health nutritional liquid for animals is prepared from lactic acid bacteria, bacillus, saccharomycetes, astragalus polysaccharide and a water extraction condensate of residue of mushroom medium in a weight ratio of 1:1:1: (0.25-0.5): (7-15), wherein the water extraction condensate of residue of mushroom medium is prepared from mushroom residues which are superfine-crushed and secondarily extracted by reflux and condensed to proportion of 1.15-1.25, and the content of the crude protein of the condensate is 38-42%, so that the nutrition of the product is extremely enhanced. The content of living probiotics of the health nutritional liquid for animals, disclosed by the invention, is high (the total living bacteria content per g is 4 billion to 7.3 billion). As a health nutritional liquid for animals with high content of living bacteria and proteins, the health nutritional liquid has the beneficial effects of promoting growth of animals, enhancing the anti-disease capacity and reducing diseases for animals, so that the economical effect of livestock and poultry industry is improved.
Description
Technical field
The present invention relates to feed additive field, be specifically related to a kind of animal health nutrient solution.
Background technology
Along with the development of livestock and poultry breeding industry, the theory of animal welfare cultivation leaned on the Mode change of the single health care of animal to be on the basis of the balance of Animal nutrition key element, regulate animal body microecological balance by the past, prevent disease is carried out to harmful microbe suppression in enteron aisle or by strengthening non-specific immune function by strengthening body, promote growth of animal, this just needs one and can effectively absorb when balanced nutrients, improve food conversion ratio, improve immunity of organisms, strengthen the resistance against diseases of animal to reduce the generation of disease, animal is made to reach healthy growth, thus improve the economic benefit of livestock and poultry breeding industry.
Summary of the invention
The object of this invention is to provide a kind of animal health nutrient solution, this health-care nutrient liquor not only effectively can regulate animal body microecological balance, strengthen body to the suppression of harmful intestinal tract microorganism, strengthen non-specific immune function so that reach prevent disease, promotion growth of animal, also have simultaneously and whet the appetite, improve food conversion ratio, main purpose of the present invention is to provide a kind of animal health nutrient solution collecting nutrition, strengthen immunologic function, regulate animal body microecological balance.
To achieve these goals, present invention employs following technical scheme:
A kind of animal health nutrient solution, is characterized in that by lactic acid bacteria (Lactobacillus), bacillus (Bacillus), saccharomycete (Saccharomyces), astragalus polyose and edible fungus bran water extracting liquid are made by the following method for 1:1:1:0.25-0.5:7-15 proportioning in mass ratio: (1) selects edible mushroom agaricus bisporus, asparagus, straw mushroom, flat mushroom, mushroom, Pleurotus nebrodensis, pleurotus eryngii, Agrocybe chaxingu, true Ji mushroom, meadow dried mushroom, Agaricus bitorqui, Stropharia rugoso-annulata, wizened mushroom, parasol mushroom, Albatrellus dispansus (Lloyd) Canf. & Gilb, black mushroom, Hericium erinaceus, phoenix-tail mushroom, coprinus comatus, HUAZIGU, Lepista sordida, grifola frondosus, Lactrarius hatsudake, Huang Jugu, Huang Liugu, pixie stool, collybia albuminosa, T.lobayense Heim, YUHUANGGU, dried mushroom, ferfas, halimasch, cepe, Fistulina hepatica (Schaeff.) Fr, the newborn bacterium of pine, matsutake, dicyclo mushroom, glossy ganoderma, white fungus, black fungus, elegant precious mushroom, hickory chick, any one bacterium chaff in Russula vinosa, or above-mentioned two or more Mixed Microbes chaff arbitrarily, drying, pulverize, cross 200-300 mesh sieve after pulverizing and obtain bacterium chaff powder, (2) bacterium chaff powder is added 40-45 DEG C of water, the weight ratio of bacterium chaff and water is 1:8-10, soaks 3-6 hour, (3) by the heating material refluxing extraction 8-12 hour after immersion, the temperature of heating and refluxing extraction is 90-95 DEG C, (4) material after heating and refluxing extraction is squeezed, obtain squeezing juice and bacterium chaff slag, (5) bacterium chaff slag is added again the water of bacterium chaff weight ratio 5 times, heating and refluxing extraction 3-5 hour, the temperature of heating and refluxing extraction is 95-100 DEG C, then squeezes, obtains squeezing juice, bacterium chaff slag separately uses it for anything else, (6) squeezing juice (4) and (5) obtained merges centrifugation, and centrifugal rotational speed 9000-16000rpm, obtains supernatant, (7) supernatant obtained is filtered, obtain filtrate, (8) by filtrate 60-90 DEG C, to be concentrated into proportion under 0.055MPa-0.1MPa condition be 1.15-1.25/40 DEG C of survey, obtains edible fungus bran water extracting liquid, (9) get the concentrate of formula ratio, add the astragalus polyose of formula ratio, in 0.7kg/cm
2, 115 DEG C of sterilizings are after 35 minutes, allow it naturally cool to less than 35 DEG C, add the lactic acid bacteria of formula ratio, bacillus and saccharomycete, be uniformly mixed, load sterile-processed after vial or plastic bottle in, sealing, obtains product.
According to the present invention, do to optimize further and/or select to such scheme.
Described lactic acid bacteria (Lactobacillus) is streptococcus thermophilus (Streptococcus thermophilus), one or more any combination in streptococcus lactis (Streptococcus lactis), Lactobacillus delbrueckii (Lactobacillus delbruekii), lactobacillus reuteri (Lactobacillus reuteri) and thermophilic Bifidobacterium Bifidum (Thermophilic Bacillus bifidus).
Described bacillus (Bacillus) is bacillus subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis), bacillus lentus (Bacillus lentus), one or more any combination in bacillus pumilus (Bacillus pumilus) and bacillus coagulans (Bacillus coagulans).
Described saccharomycete (Saccharomyces) is candida utili (Candida utilis), candida tropicalis (Candida tropicalis), brewer's yeast (Saccharomyces cerevisiae), any one in brewer's yeast (Saccharomyces cerevisiae) and fodder yeast (Candida Tropicali).
Lactic acid bacteria described above is as streptococcus thermophilus, streptococcus lactis, Lactobacillus delbrueckii, lactobacillus reuteri, thermophilic Bifidobacterium, and bacillus such as bacillus subtilis, bacillus licheniformis, bacillus lentus, bacillus pumilus and bacillus coagulans commercially available all of choice for use can be used as the feed addictive every gram viable bacteria concentration dry powder goods that are greater than 20,000,000,000 respectively.
Saccharomycete described above such as candida utili, candida tropicalis, brewer's yeast, brewer's yeast and fodder yeast can select every gram of viable bacteria concentration commercially available to be greater than the dry powder goods of 20,000,000,000.
For the streptococcus thermophilus that described above every gram is greater than 20,000,000,000 containing viable bacteria concentration, streptococcus lactis, Lactobacillus delbrueckii, lactobacillus reuteri, thermophilic Bifidobacterium, bacillus subtilis, bacillus licheniformis, bacillus lentus, bacillus pumilus, bacillus coagulans and candida utili, candida tropicalis, brewer's yeast, the thalline dry powder goods of brewer's yeast and fodder yeast, except selecting viable bacteria dry powder goods commercially available, also can cultivate under applicable condition according to existing conventional method individually according to each bacterial classification, can find from relevant handbook as condition of culture (as temperature etc.), as handbook or the textbook of the cultivation about microorganism, of the present invention easy to use in order to be applicable to, Liquid Culture Zymolysis Equipment is adopted to cultivate respectively in respective independently cultivating system by the culture medium optimized, then with supercentrifuge, the thalline in zymotic fluid is separated, be placed in dry indoor cold air drying or the drying of bacterium mud obtained by vacuum freeze drying mode, the viable bacteria concentration of every gram, the thalline dry powder obtained respectively is for being greater than 20,000,000,000.Prior art is utilized to cultivate the viable bacteria dry powder goods obtained, effectively can preserve with easy to use to goods at excellent storage environment, but, in the present invention also according to produce temporary needs but not when need preserve, also the bacterium mud that zymotic fluid adopts high speed centrifugation to separate by said method is adopted, containing the flowing product that viable bacteria is greater than 20,000,000,000 in being diluted to every gram with sterilized water, also obtain same result of use.
Oven dry described in above-mentioned steps (1) dries 12-16 hour for adopting 100-300 DEG C.
Oven dry described in above-mentioned steps (1) adopts hot-air seasoning or far infrared drying, and described pulverizing is ultramicro grinding; Preferred use vibration superfine disintegrator.
Packaging type squeezer is selected in above-mentioned steps (4) and the squeezing described in (5); Squeezing juice centrifugation described in step (6), preferably uses horizontal spiral centrifuge, preferred centrifugal rotational speed 9000-10000rpm;
Filtration described in above-mentioned steps (7) uses diatomite filtration.
In above-mentioned steps (8), preferred concentrated condition is 70-80 DEG C ,-0.065MPa ~-0.085MPa.
Concentrated described in above-mentioned steps (8) selects vacuum rotary evaporator or tubular type evaporation concentrator.
The above-described equipment of the present invention is this area conventional equipment.
Do not state the operation of its technical process in processing step in the present invention in detail, be the routine operation in the general knowledge of those skilled in the art.
Except as otherwise noted, percentage of the present invention is all weight percentage.
Edible fungus bran utilizes wood chip, cotton seed hulls, corncob, wheat bran, rice husk, waste cotton, alditol slag, xylose residue, bagasse, megasse, vinasse, rape cake and multiple kinds of crops powder of straw to carry out edible mushroom substituting stuff cultivation for primary raw material, culture medium residue after results, is commonly called as edible fungus culturing waste material, bacterium slag or clout; It is the compound of the compositions such as the crude fibre containing the residual body of hypha of edible fungus and edible mushroom enzymolysis structure generation qualitative change.China's edible and medical fungi cultivation industry development is rapid, and industry size is huge, is Edible Fungi big country of the world, is also Edible fungi exportation big country, only edible mushroom annual production about 1,100 ten thousand tons in the world.Edible and medical fungi remains cultivation base stock-i.e. bacterium chaff postpartum, and about have 20% to be converted into fructification in every hundred kilograms of siccatives, 20% comparatively becomes thermal energy consumption, and its quantity is edible and medical fungi compost 60%, is about 6,000,000 tons.Bacterium chaff has mycelium to grow in a large number, nutritive value is higher, analyze according to there being scholar, in edible fungus bran, gross protein value is about 2%-12%, crude fat average out to 0.8%-1.2%, crude fibre is on average about 14%-23%, simultaneously also containing abundant mineral matter element and the trace element such as calcium, phosphorus, zinc, copper, iron, magnesium.Medicinal fungus is as Ganoderma Lucidum sugar, and through Ganoderma Lucidum degradation, crude fibre is degraded to 23.0% from 34.5% of Medium for Ganoderma lucidum material, reduces 11.5%, and crude protein is increased to 10.2% from 5.0%, increases by 5.2%; Crude fat is increased to 4.7% from 0.40%; Crude fibre significantly reduces, and nutritive value increases substantially, dry gross energy 3.38 thermie/kilogram.For pig, digestible energy can reach 2.54 thermies/kilogram, close with the digestible energy of wheat skin, be equivalent to corn digestible energy 75%, but crude protein content is higher than corn and Mai Pi.
According to edible fungus bran contain fiber more, decompose not exclusively, many deficiency factors such as palatability is poor, nutritional utilization is insufficient, with regard to how to obtain, feed nutrient is high, fiber hydrolization is more complete, the nutrient composition content such as protein, fat is high, and edible fungus bran being obtained efficiently, fully utilizes fully is the problem that will solve at present.For this reason, the beneficiating ingredient of edible and medical fungi bacterium chaff is effectively extracted, again extract is carried out concentrated adding saccharomycete, bacillus is re-dubbed liquid-type biology feed additive, the problem that bacterium chaff fiber is difficult to absorb can not only be solved, significantly can promote the content of mycoprotein simultaneously, improve nutritive value and palatability.Bacterium chaff water extracting liquid and saccharomycete and bacillus are carried out proportioning, effectively can promote the efficiency utilization of bacterium chaff, improve the palatability of animal, make edible fungus bran provide a kind of effective approach as liquid-type feed applications.In existing disclosed patented technology, apply as nutrient feed additive with regard to edible fungus bran extract, disclose livestock and poultry edible fungus bran probiotics-containing nutrient solution and preparation method thereof (CN101731460A), its technical scheme is that edible fungus bran is carried 2-3 hour through poach, isolated by filtration gets filtrate, then bacterium chaff extract is added other nutriment more respectively and prepare fermentation culture I and II respectively, add in bacterium chaff digestion liquid by fermentation culture respectively again, within 5-7 days, fermentation culture I is obtained 32-37 DEG C of cultivation, again fermentation culture II is added in bacterium bacterium digestion liquid, within 2-3 days, fermentation culture II is obtained 24-28 DEG C of cultivation, and then fermentation culture I and II is mixed than 2:1 according to body journey, packing is made, can find out from prior art and utilize the edible fungus bran digestion liquid probiotics-containing nutrient solution made of fermented and cultured respectively, the metabolite that its bacterial classification produces after fermented and cultured can improve the nutritive effect of its nutrient solution, but it is imprecise with regard to its probio content, easy aging decline is its defect, the effect of probio contained by nutrient solution can not be ensured.
Astragalus polyose is by uronic acid, glucose, fructose, rhamnose, arabinose, galacturonic acid and glucuronic acid etc. form, these active components all have enhancing antibody to generate and immunoreactive effect, can be used as immunopotentiating agent and conditioning agent, can significantly strengthen non-specific immune function and humoral immune function.
Beneficial effect of the present invention:
(1) because the present invention takes to carry out ultramicro grinding by after the oven dry of bacterium chaff, make the hyphal cell wall of bacterium chaff damaged, soaking a period of time is beneficial to nutritional labeling stripping, a large amount of mycoprotein, amino acid, carbohydrate is made to obtain fully or completely extracting in refluxing extraction process, simultaneously by cellulose components after ultramicro grinding, also effectively nutriment contained in cellulose effectively can be extracted, be promoted the trophic function of extract.
(2) the present invention makes the nutrition in bacterium chaff be extracted to greatest extent owing to bacterium chaff Ultramicro-powder to be carried out secondary back extraction, and the cellulose composition of indissoluble is given filtering, its extract is through concentrated, the nutritions such as the protein in product, amino acid are significantly improved, and the animal health nutrient solution crude protein content that the inventive method obtains is 38-42%.
(3) owing to present invention employs secondary back extraction and concentration technique, mycoprotein content is high, not only significant increase trophism, also improve palatability and attractant greatly simultaneously, because the present invention adopts Effect of Alcohol Extracting Procedureto Total, make manufactured goods good water solubility, be beneficial to absorption and the utilization of animal, enhance the result of use of product.
(4) probio content of the present invention is high, the total viable bacteria content of compound bacteria 4,000,000,000-73 hundred million/gram, and product is stablized, define the compound viable bacteria goods of lactic acid bacteria, bacillus, saccharomycete and bacterium chaff protein concentration, on the one hand as high-protein nutrient agent, the short long-acting fruit of animal can be improved and then strengthen the defending and fighting against diseases ability of body, the control of antibiotic for disease of digestive system be can partly replace or reduce simultaneously, and lactic acid bacteria, bacillus and saccharomycete shared enhancing livestock and poultry Abwehrkraft des Koepers, the pathogenetic beneficial effect of minimizing disease played.
(5) the aqueous health-caring feedstuff addictive of the present invention is used for mix feed, contained probio can be made to be attached on feed surface, being beneficial to probio and playing effect and utilization rate, add in livestock and fowl drinking water simultaneously, livestock and poultry can being made when drinking water to obtain the needs of probio and extra-nutrition.
Embodiment 1
Use corresponding Optimal Medium, individually streptococcus thermophilus is cultivated by liquid fermentation equipment, streptococcus lactis, Lactobacillus delbrueckii, lactobacillus reuteri and thermophilic Bifidobacterium, then with supercentrifuge, the thalline in zymotic fluid is separated, by vacuum freeze drying mode by dry for the bacterium mud separated, to obtain viable bacteria content be respectively 25,000,000,000/gram streptococcus thermophilus dried powder, viable bacteria content is 25,000,000,000/gram streptococcus lactis dry powder, viable bacteria content is 25,000,000,000/gram Lactobacillus delbrueckii dry powder, viable bacteria content is 25,000,000,000/gram lactobacillus reuteri dry powder and viable bacteria content be 25,000,000,000/gram thermophilic bifidobacteria dry powder.
Embodiment 2
Use corresponding Optimal Medium, individually bacillus subtilis is cultivated by liquid fermentation equipment, bacillus licheniformis, De Shi bacillus, lactobacillus reuteri and thermophilic Bifidobacterium, then with supercentrifuge, the thalline in zymotic fluid is separated, by vacuum freeze drying mode by dry for the bacterium mud separated, to obtain viable bacteria content be respectively 25,000,000,000/gram bacillus subtilis dry powder, viable bacteria content is 25,000,000,000/gram bacillus licheniformis dry powder, viable bacteria content is 25,000,000,000/gram Lactobacillus delbrueckii dry powder, viable bacteria content is 25,000,000,000/gram lactobacillus reuteri dry powder, viable bacteria content is 25,000,000,000/gram thermophilic bifidobacteria dry powder.
Embodiment 3
Use corresponding Optimal Medium, candida utili, candida tropicalis, brewer's yeast, brewer's yeast and fodder yeast is individually cultivated by liquid fermentation equipment, then with supercentrifuge, the thalline in zymotic fluid is separated, by vacuum freeze drying mode by dry for the bacterium mud separated, to obtain viable bacteria content be respectively 25,000,000,000/gram candida utili dry powder, viable bacteria content be 25,000,000,000/gram candida tropicalis dry powder, viable bacteria content be 25,000,000,000/gram brewer's yeast dry powder and viable bacteria content be 25,000,000,000/gram feed yeast powder.
Embodiment 4
(1) get edible mushroom pleurotus eryngii bacterium chaff, smash, under the condition of 300 DEG C, dry 12 hours with hot air drier, obtain the dry slag of bacterium chaff, dry slag is cooled to room temperature, use vibration superfine disintegrator to pulverize, the bacterium chaff powder of pulverizing crosses 300 mesh sieves, obtains bacterium chaff powder; (2) take 100 kilograms, bacterium chaff powder, add the hot water 800 kilograms of 40-45 DEG C, soak 3 hours; (3) by heating material to the 95 DEG C refluxing extraction 8 hours after soaking; (4) the material packaging type squeezer after heating and refluxing extraction is squeezed, obtain squeezing juice and bacterium chaff slag; (5) bacterium chaff slag is added the water of 500 kilograms again, be heated to 100 DEG C of refluxing extraction 3 hours, then adopt packaging type squeezer to squeeze, obtain squeezing juice; (6) squeezing juice (4) and (5) obtained respectively merges, and use horizontal centrifuge centrifugation, centrifugal rotational speed 9000rpm, obtains supernatant; (7) supernatant obtained is added 6.5 kilograms of diatomite, filter with diatomite filter, obtain filtrate; (8) by filtrate vacuum rotary evaporator temperature be 70 DEG C, vacuum for concentrated under-0.085Mpa condition, being concentrated into proportion is 1.15, obtains concentrate; (9) get concentrate 7 kilograms and add astragalus polyose 0.25 kilogram, stir, in 0.7kg/cm
2115 DEG C of sterilizings are after 35 minutes, to be cooled to less than 35 DEG C time, add 1 kilogram, the bacillus subtilis dry powder obtained by streptococcus thermophilus dried powder 1 kilogram, embodiment 2 that embodiment 1 obtains and the false yeast dry powder of product protein 1 kilogram of mixing and stirring that embodiment 3 obtains, be sub-packed in the dry glass bottle after disinfecting, sealing bottleneck, obtains product.After testing, crude protein content is 42.62% to this product; Total probiotics viable bacteria content is 73.17 hundred million/gram.
Embodiment 5
(1) get edible mushroom asparagus wood chip bacterium chaff, smash, dry 14 hours, obtain the dry slag of bacterium chaff, dry slag is cooled to room temperature under the condition of 200 DEG C with hot air conveying type drying box, use vibration superfine disintegrator to pulverize, the bacterium chaff powder of pulverizing crosses 200 mesh sieves, obtains bacterium chaff powder; (2) take 100 kilograms, bacterium chaff powder, add the hot water 900 kilograms of 40-45 DEG C, soak 6 hours; (3) by heating material to the 90 DEG C refluxing extraction 12 hours after soaking; (4) the material packaging type squeezer after heating and refluxing extraction is squeezed, obtain squeezing juice and bacterium chaff slag; (5) bacterium chaff slag is added the water of 500 kilograms again, be heated to 95 DEG C of refluxing extraction 5 hours, then adopt packaging type squeezer to squeeze, obtain squeezing juice; (6) squeezing juice (4) and (5) obtained respectively merges, and use horizontal centrifuge centrifugation, centrifugal rotational speed 10000rpm, obtains supernatant; (7) supernatant obtained is added 7 kilograms of diatomite, filter with diatomite filter, obtain filtrate; (8) by filtrate vacuum rotary evaporator temperature be 80 DEG C, vacuum for concentrated under-0.065Mpa condition, being concentrated into proportion is 1.20, obtains concentrate; (9) get concentrate 15 kilograms and add astragalus polyose 0.5 kilogram, stir, in 0.7kg/cm
2115 DEG C of sterilizings are after 35 minutes, be cooled to less than 35 DEG C, add 0.5 kilogram, streptococcus lactis dry powder that embodiment 1 obtains, 1 kilogram, candida tropicalis dry powder that 0.5 kilogram, bacillus licheniformis dry powder, 0.5 kilogram, bacillus lentus dry powder and embodiment 3 that 0.5 kilogram, lactobacillus reuteri dry powder, embodiment 2 obtain obtain, be uniformly mixed, be sub-packed in the dry plastics bottle after disinfecting, sealing bottleneck, obtains product.After testing, crude protein content is 40.02% to this product; Total probiotics viable bacteria content is 40.54 hundred million/gram.
Embodiment 6
(1) take food and smash mixing respectively with Ganoderma wood chip bacterium chaff and white fungus corncob bacterium chaff, under the condition of 150 DEG C, dry 15 hours with hot air conveying type drying box, obtain the dry slag of bacterium chaff, dry slag is cooled to room temperature, use vibration superfine disintegrator is pulverized, and the bacterium chaff powder of pulverizing crosses 250 mesh sieves, obtains bacterium chaff powder, (2) take Mixed Microbes chaff 100 kilograms, add the hot water 900 kilograms of 40-45 DEG C, soak 5 hours, (3) by heating material to the 95 DEG C refluxing extraction 8 hours after soaking, (4) the material packaging type squeezer after heating and refluxing extraction is squeezed, obtain squeezing juice and bacterium chaff slag, (5) bacterium chaff slag is added the water of 500 kilograms again, be heated to 95 DEG C of refluxing extraction 5 hours, then adopt packaging type squeezer to squeeze, obtain squeezing juice, (6) squeezing juice (4) and (5) obtained respectively merges, and use horizontal centrifuge centrifugation, centrifugal rotational speed 9500rpm, obtains supernatant, (7) supernatant obtained is added 7 kilograms of diatomite, filter with diatomite filter, obtain filtrate, (8) by filtrate vacuum rotary evaporator temperature be 75 DEG C, vacuum for concentrated under-0.075Mpa condition, being concentrated into proportion is 1.20/40 DEG C of survey, obtains concentrate, (9) get concentrate 10 kilograms and add astragalus polyose 0.3 kilogram, stir, in 0.75kg/cm
2115 DEG C of sterilizings are after 35 minutes, be cooled to less than 35 DEG C, add the streptococcus thermophilus dried powder 0.2 kilogram that embodiment 1 obtains, 0.2 kilogram, streptococcus lactis dry powder, 0.2 kilogram, Lactobacillus delbrueckii dry powder, 0.2 kilogram, lactobacillus reuteri dry powder, thermophilic bifidobacteria dry powder 0.2 kilogram, 0.2 kilogram, the bacillus subtilis dry powder that embodiment 2 obtains, 0.2 kilogram, bacillus licheniformis dry powder, 0.2 kilogram, bacillus lentus dry powder, 0.2 kilogram, bacillus pumilus dry powder, the feed yeast powder 1 kilogram that 0.2 kilogram, bacillus coagulans dry powder and embodiment 3 obtain, mixing and stirring, be sub-packed in the dry plastics bottle after disinfecting, sealing bottleneck, obtain product.After testing, crude protein content is 38.52% to this product; Total probiotics viable bacteria content is 56.39 hundred million/gram.
Embodiment 7
(1) get edible mushroom mushroom wood chip bacterium chaff, smash and dry 16 hours with hot air conveying type drying box under the condition of 100 DEG C, obtain the dry slag of bacterium chaff, dry slag is cooled to room temperature, use vibration superfine disintegrator to pulverize, the bacterium chaff powder of pulverizing crosses 200 mesh sieves, obtains bacterium chaff powder; (2) take 100 kilograms, bacterium chaff powder, add the hot water 900 kilograms of 40-45 DEG C, soak 5 hours; (3) by heating material to the 90 DEG C refluxing extraction 10 hours after soaking; (4) the material packaging type squeezer after heating and refluxing extraction is squeezed, obtain squeezing juice and bacterium chaff slag; (5) bacterium chaff slag is added the water of 500 kilograms again, be heated to 100 DEG C of refluxing extraction 3 hours, then adopt packaging type squeezer to squeeze, obtain squeezing juice; (6) squeezing juice (4) and (5) obtained respectively merges, and use horizontal centrifuge centrifugation, centrifugal rotational speed 10000rpm, obtains supernatant; (7) supernatant obtained is added 7 kilograms of diatomite, filter with diatomite filter, obtain filtrate; (8) by filtrate vacuum rotary evaporator temperature be 78 DEG C, vacuum for concentrated under-0.07Mpa condition, being concentrated into proportion is 1.20/40 DEG C of survey, obtains concentrate; (9) get concentrate 12 kilograms and add astragalus polyose 0.4 kilogram, stir, in 0.7kg/cm
2115 DEG C of sterilizings are after 35 minutes, to be cooled to less than 35 DEG C time, add 0.25 kilogram, streptococcus lactis dry powder that embodiment 1 obtains, brewer's yeast dry powder 1 kilogram of mixing and stirring that 0.5 kilogram, bacillus subtilis dry powder, 0.5 kilogram, bacillus licheniformis dry powder and embodiment 3 that 0.25 kilogram, lactobacillus reuteri dry powder, thermophilic bifidobacteria dry powder 0.25 kilogram, streptococcus thermophilus dried powder 0.25 kilogram, embodiment 2 obtain obtain, be sub-packed in sterile-processed after dry glass bottle in, sealing bottleneck, obtains product.After testing, crude protein content is 41.74% to this product; Total probio content is 48.70 hundred million/gram.
Embodiment 8
(1) get edible mushroom flat mushroom cotton seed hulls bacterium chaff, smash and dry 13 hours with hot air conveying type drying box under the condition of 250 DEG C, obtain the dry slag of bacterium chaff, dry slag is cooled to room temperature, use vibration superfine disintegrator to pulverize, the bacterium chaff powder of pulverizing crosses 250 mesh sieves, obtains bacterium chaff powder; (2) take 100 kilograms, bacterium chaff powder, add the hot water 900 kilograms of 40-45 DEG C, soak 6 hours; (3) by heating material to the 95 DEG C refluxing extraction 8 hours after soaking; (4) the material packaging type squeezer after heating and refluxing extraction is squeezed, obtain squeezing juice and bacterium chaff slag; (5) bacterium chaff slag is added the water of 500 kilograms again, be heated to 100 DEG C of refluxing extraction 5 hours, then adopt packaging type squeezer to squeeze, obtain squeezing juice; (6) squeezing juice (4) and (5) obtained respectively merges, and use horizontal centrifuge centrifugation, centrifugal rotational speed 10000rpm, obtains supernatant; (7) supernatant obtained is added 7 kilograms of diatomite, filter with diatomite filter, obtain filtrate; (8) by filtrate vacuum rotary evaporator temperature be 70 DEG C, vacuum for concentrated under-0.085Mpa condition, being concentrated into proportion is 1.15, obtains concentrate; (9) get concentrate 8 kilograms and add astragalus polyose 0.375 kilogram, stir, in 0.7kg/cm
2, 115 DEG C of sterilizings 35 minutes, add streptococcus thermophilus 0.5 kilogram that embodiment 1 obtains during less than 35 DEG C, ground to be cooled, brewer's yeast 1 kilogram of mixing and stirring that bacillus subtilis 0.25 kilogram that thermophilic Bifidobacterium 0.5 kilogram, embodiment 2 obtain, bacillus licheniformis 0.25 kilogram, bacillus lentus 0.25 kilogram, bacillus pumilus 0.25 kilogram and embodiment 3 obtain, be sub-packed in sterile-processed after dry glass bottle in, sealing bottleneck, obtains product.After testing, crude protein content is 41.96% to this product; Total probiotics viable bacteria content is 65.93 hundred million/gram.
Claims (11)
1. an animal health nutrient solution, is characterized in that by lactic acid bacteria (Lactobacillus), bacillus (Bacillus), saccharomycete (Saccharomyces), astragalus polyose and edible fungus bran water extracting liquid are made by the following method for 1:1:1:0.25-0.5:7-15 proportioning in mass ratio: (1) selects edible mushroom agaricus bisporus, asparagus, straw mushroom, flat mushroom, mushroom, Pleurotus nebrodensis, pleurotus eryngii, Agrocybe chaxingu, true Ji mushroom, meadow dried mushroom, Agaricus bitorqui, Stropharia rugoso-annulata, wizened mushroom, parasol mushroom, Albatrellus dispansus (Lloyd) Canf. & Gilb, black mushroom, Hericium erinaceus, phoenix-tail mushroom, coprinus comatus, HUAZIGU, Lepista sordida, grifola frondosus, Lactrarius hatsudake, Huang Jugu, Huang Liugu, pixie stool, collybia albuminosa, T.lobayense Heim, YUHUANGGU, dried mushroom, ferfas, halimasch, cepe, Fistulina hepatica (Schaeff.) Fr, the newborn bacterium of pine, matsutake, dicyclo mushroom, glossy ganoderma, white fungus, black fungus, elegant precious mushroom, hickory chick, any one bacterium chaff in Russula vinosa or arbitrarily two or more Mixed Microbes chaff, drying, pulverize, cross 200-300 mesh sieve after pulverizing and obtain bacterium chaff powder, (2) bacterium chaff powder is added 40-45 DEG C of water, the weight ratio of bacterium chaff and water is 1:8-10, soaks 3-6 hour, (3) by the heating material refluxing extraction 8-12 hour after immersion, the temperature of heating and refluxing extraction is 90-95 DEG C, (4) material after heating and refluxing extraction is squeezed, obtain squeezing juice and bacterium chaff slag, (5) bacterium chaff slag is added again the water of bacterium chaff weight ratio 5 times, heating and refluxing extraction 3-5 hour, the temperature of heating and refluxing extraction is 95-100 DEG C, then squeezes, obtains squeezing juice, (6) squeezing juice (4) and (5) obtained merges centrifugation, and centrifugal rotational speed 9000-16000rpm, obtains supernatant, (7) supernatant obtained is filtered, obtain filtrate, (8) by filtrate 60-90 DEG C, to be concentrated into proportion under 0.055MPa-0.1MPa condition be 1.15-1.25/40 DEG C of survey, obtains edible fungus bran water extracting liquid, (9) get the concentrate of formula ratio, add the astragalus polyose of formula ratio, in 0.7kg/cm
2, 115 DEG C of sterilizings are after 35 minutes, allow it naturally cool to less than 35 DEG C, add the lactic acid bacteria of formula ratio, bacillus and saccharomycete, be uniformly mixed, load sterile-processed after vial or plastic bottle in, sealing bottleneck packaging, obtains product.
2. a kind of animal health nutrient solution as claimed in claim 1, is characterized in that: described lactic acid bacteria (Lactobacillus) is streptococcus thermophilus (Streptococcus thermophilus), one or more any combination in streptococcus lactis (Streptococcus lactis), Lactobacillus delbrueckii (Lactobacillus delbruekii), lactobacillus reuteri (Lactobacillus reuteri) and thermophilic Bifidobacterium Bifidum (Thermophilic Bacillus bifidus).
3. a kind of animal health nutrient solution as claimed in claim 1, is characterized in that: described bacillus (Bacillus) is bacillus subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis), bacillus lentus (Bacillus lentus), one or more any combination in bacillus pumilus (Bacillus pumilus) and bacillus coagulans (Bacillus coagulans).
4. a kind of animal health nutrient solution as claimed in claim 1, is characterized in that: described saccharomycete (Saccharomyces) is candida utili (Candida utilis), candida tropicalis (Candida tropicalis), brewer's yeast (Saccharomyces cerevisiae), any one in brewer's yeast (Saccharomyces cerevisiae) and fodder yeast (Candida Tropicali).
5. a kind of animal health nutrient solution according to claim 1, is characterized in that: the oven dry described in described step (1) is for adopting hot-air seasoning or far infrared drying; Drying condition dries 12-16 hour for adopting 100-300 DEG C; Described pulverizing is ultramicro grinding.
6. a kind of animal health nutrient solution according to claim 5, is characterized in that: described ultramicro grinding uses vibration superfine disintegrator.
7. a kind of animal health nutrient solution according to claim 1, is characterized in that: packaging type squeezer is selected in described step (4) and the squeezing described in (5); Described in step (6), preferably horizontal spiral centrifuge is used to squeezing juice centrifugation; Preferred centrifugal rotational speed is 9000-10000rpm.
8. a kind of animal health nutrient solution according to claim 1, is characterized in that: the filtration described in described step (7) uses diatomite filtration.
9. a kind of animal health nutrient solution according to claim 1, is characterized in that: described step (8) preferably concentrated condition is 70-80 DEG C ,-0.065MPa ~-0.085MPa; Preferred use vacuum rotary evaporator or tubular type evaporation concentrator.
10. the preparation method of the animal health nutrient solution as described in any one of claim 1-10.
11. a kind of animal health nutrient solutions according to claim 1, is characterized in that: the crude protein content of described animal health nutrient solution is 38-42%; Total viable bacteria content be 40-73 hundred million/gram.
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| CN105265829A (en) * | 2015-10-12 | 2016-01-27 | 珠海海龙生物科技有限公司 | Feed for preventing grass carp liver tissue fat abnormal deposition |
| CN106036024A (en) * | 2016-06-27 | 2016-10-26 | 固镇县牧兴生态养猪场 | Preparation method of cooling and refreshing drinking water drunk by pig after movement |
| CN106071079A (en) * | 2016-06-27 | 2016-11-09 | 固镇县牧兴生态养猪场 | The preventing heatstroke beverage drunk after swinery motion |
| CN106071560A (en) * | 2016-06-27 | 2016-11-09 | 固镇县牧兴生态养猪场 | The preparation method of the preventing heatstroke beverage drunk after swinery motion |
| CN106173379A (en) * | 2016-06-27 | 2016-12-07 | 固镇县牧兴生态养猪场 | The summer-heat removing drinking water drunk after pig moves |
| CN106359901A (en) * | 2016-08-29 | 2017-02-01 | 青岛润达生物科技有限公司 | Compound product of glossy ganoderma polysaccharide and lactic acid bacteria |
| CN106754432A (en) * | 2017-02-16 | 2017-05-31 | 江苏中祥高科技实业有限公司 | A kind of production method of ganoderma lucidum complex immunity agent |
| CN107484884A (en) * | 2017-08-10 | 2017-12-19 | 浙江绿美生物科技有限公司 | A kind of feed addictive of high nutrition |
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Application publication date: 20150408 |