CN106615927A - Broiler chicken feed additive based on bacillus coagulans and application thereof - Google Patents
Broiler chicken feed additive based on bacillus coagulans and application thereof Download PDFInfo
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- CN106615927A CN106615927A CN201611102456.4A CN201611102456A CN106615927A CN 106615927 A CN106615927 A CN 106615927A CN 201611102456 A CN201611102456 A CN 201611102456A CN 106615927 A CN106615927 A CN 106615927A
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Abstract
The invention relates to the field of broiler chicken feed additives, and especially relates to a broiler chicken feed additive based on bacillus coagulans and application thereof. The feed additive comprises the following components in percent by weight: 2%-10% of bacillus coagulans, 1%-5% of acidifier, 5%-20% of complex enzyme preparation, and 65%-89% of carrier. In the invention, the acidifier, the bacillus coagulans, the complex enzyme preparation, an emulsifying agent and the carrier are added into a low-energy daily ration, so that the acidifier and carrier can wrap the bacillus coagulans and helps to protect the bacillus coagulans in gastric fluid of broiler chickens from corrosion and impact of gastric acid and keep the bacillus coagulans active, so as to improve intestinal microflora balance and pathological loss of feed; after entering the intestine, the complex enzyme preparation and the emulsifying agent can help to improve the utilization ratio of energy substances such as fats and the like, alleviate trophic diarrhea caused by indigestion of fats and reduce trophic loss of feed. The additive added into the feed helps to achieve the purpose of reducing trophic loss of feed and pathological loss of feed.
Description
Technical field
The present invention relates to feed additive field, especially a kind of feed additive for chickens based on bacillus coagulans
And the low energy feed containing this feed addictive, the invention further relates to a kind of meat chicken feed based on bacillus coagulans adds
Application of the agent in terms of broiler fodder.
Background technology
The broiler breeding of China was risen the eighties in last century, through the fast development of nearly more than 30 years, progressively to it is intensive,
Scale, the direction of degree of specialization are developed, and fast large-scale broiler chicken is because of its fast growth, and cycle is short, the price of deed is high, increasingly
To the favor of market and raiser.But, due to excessively pursuing broiler chicken quickly cultivation and too high productivity effect, consequently also bring
A series of problem：Such as, 1, because the speed of weight increment of broiler chicken is too fast, cause the resistance of broiler chicken reply disease poor, easily fall ill,
So that breeding cycle innerlich anwenden is excessive；2nd, high density feed, plant's air quality is poor, and environmental pollution is serious；3rd, broiler chicken
It is universal under hot and humid environment after excessive feeding " punishment in advance " phenomenon easily occur, cause growth performance to reduce, efficiency of feed utilization drop
Low, cultivation comprehensive benefit is reduced.
In the problems referred to above, most generally, while also the problem of most serious is broiler chicken " punishment in advance " phenomenon, " punishment in advance " refers to broiler chicken excrement
Just there are indigested feed granules in, show as that excrement is shapeless, outer attached significant white lithate；Containing Huang in excrement
Color is macroscopic not to digest feed granules, there is watery stool sometimes.Jing is analyzed, and causing the factor of broiler chicken " punishment in advance " phenomenon may have
It is following several：1st, " punishment in advance " that nutritive disease causes, obvious to be characterized in that：Formed stools degree is poor, morbidity chicken aberration,
The price of deed is low, deliver for sale again mitigate, colony's uniformity it is inconsistent, clinical manifestation：Common glandular stomach, muscular stomach and injury of small intestine.May
Due to VAD, fodder energy not enough or indigestion causes after extra addition grease in daily ration；2nd, infectiousness disease
" punishment in advance " that disease causes, many causes the disease of intestinal tract injury to cause the flora imbalance of Intestine of Broiler, so as to cause transience
Diarrhoea or " punishment in advance " phenomenon, such as：The infectious disease disease such as bird flu, tissue trichomonad.For a long time " punishment in advance " can cause growth of meat chicken
Energy and plant recovery of nutrient are reduced, and feed conversion rate is reduced, and productivity effect is impaired serious, cause huge economy to damage to raiser
Lose.
Bacillus coagulans belongs to facultative anaerobic, i.e., can grow under aerobic and oxygen free condition, adapts to the low of enteron aisle
Oxygen environment, biocidal property is strong.Jing is studied, the health care of the beneficial bacterium such as the existing Bifidobacterium of bacillus coagulans and lactic acid bacteria, together
When have higher tolerance performance to hydrochloric acid in gastric juice and bile, primary efficacy is as follows：1st, animal alimentary canal balance is adjusted；2nd, animal machine is promoted
Body is digested and assimilated to nutriment；3rd, animal body immunity and resistance against diseases are improved.Then, based on above-mentioned effect, there is
Person attempts directly adding bacillus coagulans in daily ration of broiler, it is intended to adjusts Intestine of Broiler flora by bacillus coagulans and puts down
Weighing apparatus, reaching improves the purpose of " punishment in advance " phenomenon, while improving meat chicken growth performance, feed conversion rate and cultivation comprehensive benefit, drop
Low feed cost.
But, through substantial amounts of Animal experiment, result of the test shows：Add bacillus coagulans in daily ration of broiler
Afterwards, just just phenomenon does not substantially mitigate the water for bringing because of " punishment in advance " with feed, i.e., bacillus coagulans does not exist well
Playing in Intestine of Broiler significantly improves the effect that enteron aisle reduces " punishment in advance ".Jing is analyzed, and is the reason for possible：1st, gemma bar is condensed
Though bacterium is with stronger acidproof and bile-tolerance energy, if it is not specially treated its when this particular surroundings of broiler chicken intestines and stomach,
Stomach acid attack is easily received, causes its activity to substantially reduce, its normal effect cannot be played into after Intestine of Broiler；2nd, it is likely to be battalion
Nourish one's nature " punishment in advance ", if simply simple addition bacillus coagulans improves intestinal environment, it is also possible to which not reaching improves trophism " mistake
The effect of material ", also just cannot mitigate water just with feed just phenomenon.
The content of the invention
After overcoming the problems referred to above, the present invention to society's offer one kind bacillus coagulans can be made to enter in broiler chicken stomach
The activity for be not easily susceptible to stomach acid attack, making bacillus coagulans is maintained, hence into can be abundant after in the enteron aisle to broiler chicken
Playing it improves effect of enteron aisle, and with this feed addictive for mitigating disease " punishment in advance " phenomenon of broiler chicken significantly is reached.
In addition, another beneficial effect of the present invention is, the complex enzyme formulation and emulsifying agent in the present invention enters into meat
In chicken intestinal, it is possible to increase the utilization rate of the energy matter such as grease type, improve the nutrition that oil substances are caused because of indigestion
Property diarrhoea, improve broiler chicken trophism " punishment in advance " phenomenon.
The present invention also provides a kind of feed additive for chickens based on bacillus coagulans answering in terms of broiler fodder
With.
The technical scheme is that：A kind of feed additive for chickens based on bacillus coagulans is provided, including under
State the component of percentage by weight：Bacillus coagulans 2%-10%, acidulant 1%-5%, complex enzyme formulation 5%-20%, carrier 65%-
89%。
As improvement of the present invention, including the component of following weight percentage：Bacillus coagulans 4%-8%, acidulant
2%-4%, complex enzyme formulation 9%-16%, carrier 75%-79%.
As improvement of the present invention, also including the emulsifying agent that percentage by weight is 2%-10%.
As improvement of the present invention, wherein, the complex enzyme formulation includes the component of following weight percentage：Lipase
10%-20%, zytase 10%-20%, dextranase 10%-20%, mannase 5%-10%, amylase 5%-10%, acid egg
White enzyme 5%-10%, alkali protease 10%-20%, galactosidase 5%-10%.
As improvement of the present invention, wherein, the acidulant includes the component of following weight percentage：Formic acid 30%-
40%th, propionic acid 20%-30%, orthophosphoric acid 30%-40%.
The present invention also provides a kind of broiler fodder, and the feed contains any one above-mentioned feed addictive, feed addictive
0.01%-0.03%, base-material 99.7%-99.9%.
Used as improvement of the present invention, the base-material is basal diet or low energy daily ration.
As improvement of the present invention, wherein, the basal diet includes the component of following weight percentage：Corn 50%-
65%, dregs of beans 15%-30%, corn protein powder 3%-6%, vinasse albumen feed (DDGS) 4%-6%, level Four soybean oil 2%-6%, wheat-middlings
0.1%-5%, stone flour 1.3%-1.6%, calcium monohydrogen phosphate 0.6%-1.5%, salt 0.3%-0.4%, lysine 0.3%-0.5%, solid egg
The premix 1.0% of propylhomoserin 0.4%-1.2%, threonine 0.2%-0.8%, first.
As improvement of the present invention, wherein, the low energy daily ration includes the component of following weight percentage：Corn 55%-
65%, dregs of beans 14%-28%, corn protein powder 4%-5%, vinasse albumen feed (DDGS) 5%-6%, level Four soybean oil 0.4%-4%,
Wheat-middlings 0.1%-5%, stone flour 1.3%-1.5%, calcium monohydrogen phosphate 0.6%-1.5%, salt 0.3%-0.4%, lysine 0.3%-0.5%, Gu
The premix 1.0% of body methionine 0.03%-1.2%, threonine 0.02%-0.08%, second.
The present invention also provides a kind of feed additive for chickens based on bacillus coagulans answering in terms of broiler fodder
With.
In the present invention, first bacillus coagulans and acidulant and carrier are premixed, make bacillus coagulans be acidified agent bag
Wrap, after broiler chicken has searched for food the daily ration containing this additive, bacillus coagulans is entered in broiler chicken stomach due to the parcel of acidulant
Effect is activity stabilized from hydrochloric acid in gastric juice impact, can play a part of to adjust gastrointestinal bacterial flora balance into after Intestine of Broiler, while sour
The pH value of the adjustable broiler chicken gastric content of agent, cooperates with the acid resistance of bacillus coagulans, improves digestive tract enzyme activity and nutrition
The effect of substance digestion rate；Meanwhile, the acid condition of acidulant manufacture has using the flourish of lactic acid bacteria, while to large intestine bar
The harmful microbe inhibitory action such as bacterium, beneficial to beneficial bacterium breeding；Body intracellular metabolite is directly participated in, strengthens human body immune function,
Alleviation stress.Meanwhile, the complex enzyme formulation contained in the feed addictive and emulsifying agent are entered after Intestine of Broiler, can improve grease
The utilization rate of the energy matters such as class, reduces sending out for the trophism diarrhea that causes because of indigestion of oil substances and fatty liver
It is raw, improve trophism " punishment in advance ".Therefore, the present invention has and enables to after bacillus coagulans is entered in broiler chicken stomach from hydrochloric acid in gastric juice
Invade so that the activity of bacillus coagulans is maintained, its effect can be given full play to after entering in the enteron aisle of broiler chicken, so as to reach
To regulation Intestine of Broiler colony balance, promote purpose of the Intestine of Broiler to the digestion of feed.Meanwhile, the complex enzyme system in the present invention
Agent and emulsifying agent can improve Lipid use rate, reduce the generation of trophism diarrhea and fatty liver, improve trophism " punishment in advance " phenomenon.
The present invention can significantly mitigate disease " punishment in advance " and trophism " punishment in advance " phenomenon of broiler chicken simultaneously.
In the present invention, first acidulant, bacillus coagulans, complex enzyme formulation, emulsifying agent and carrier are premixed, make acidulant
Bacillus coagulans, complex enzyme formulation, emulsifying agent are wrapped with carrier, when broiler chicken has been searched for food the low energy feed containing this additive
Afterwards, the pH value of the adjustable broiler chicken gastric content of acidulant, corroding the gastric juice that the bacillus coagulans being wrapped is subject to reduces,
Its loss of activity is reduced, the effect of its regulating intestinal canal flora can be normally played into after Intestine of Broiler, improve broiler chicken immunity of organism
Power and premunition, reduce intestines problem, improve disease " punishment in advance ".Meanwhile, agent and carrier bag are acidified in the feed addictive
The complex enzyme formulation wrapped up in and emulsifying agent are entered after Intestine of Broiler, can effectively improve the utilization rate of grease type energy matter, reduce oil
The trophism diarrhea that lipid material is caused because of indigestion, reaching improves the purpose of trophism " punishment in advance ".Feed in the present invention
Additive can be effectively improved trophism " punishment in advance " and disease " punishment in advance ", improve broiler chicken resistance against diseases, reach raising culture benefit
Purpose.
Specific embodiment
In the present invention, complex enzyme formulation therein includes the component of following weight percentage, each embodiment specific as follows：
Embodiment 1
Lipase 10%, zytase 19%, dextranase 20%, mannase 10%, amylase 8 %, acid protease 10%, alkali
Property protease 16%, galactosidase 7%.
Embodiment 2
Lipase 16%, zytase 16%, dextranase 16%, mannase 7%, amylase 7%, acid protease 8%, alkalescence
Protease 20%, galactosidase 10%.
Embodiment 3
Lipase 20%, zytase 20%, dextranase 14%, mannase 8%, amylase 10%, acid protease 7%, alkali
Property protease 14%, galactosidase 7%.
Embodiment 4
Lipase 14%, zytase 14%, dextranase 20%, mannase 7%, amylase 5%, acid protease 10%, alkali
Property protease 20%, galactosidase 10%.
Embodiment 5
Lipase 20%, zytase 10%, dextranase 20%, mannase 5%, amylase 7%, acid protease 10%, alkali
Property protease 20%, galactosidase 8%.
Embodiment 6
Lipase 20%, zytase 20%, dextranase 15%, mannase 10%, amylase 10%, acid protease 5%, alkali
Property protease 10%, galactosidase 10%.
Embodiment 7
Lipase 20%, zytase 20%, dextranase 10%, mannase 10%, amylase 10%, acid protease 10%,
Alkali protease 15%, galactosidase 5%.
In the present invention, in any embodiment in embodiment 1 to 7, the enzyme activity scope of the lipase be more than or equal to
10000U/g is less than or equal to 20000U/g, and the enzyme activity scope of zytase is to be less than or equal to 2000U/g more than or equal to 1000U/g,
The enzyme activity scope of dextranase be more than or equal to 500U/g less than or equal to 1000U/g, the enzyme activity scope of mannase be more than
1000U/g is less than or equal to equal to 500U/g, diastatic enzyme activity scope is less than or equal to 2000U/g, acid more than or equal to 1000U/g
Property protease enzyme activity scope be more than or equal to 1000U/g less than or equal to 2000U/g, the enzyme activity scope of alkali protease be more than
2000U/g is less than or equal to equal to 1000U/g, the enzyme activity scope of galactosidase is to be less than or equal to more than or equal to 1000U/g
2000U/g；Wherein, the lipase, zytase, dextranase, mannonase amylase, acid protease, alkaline egg
White enzyme and galactosidase are all solid-states.
In the present invention, acidulant therein includes the component of following weight percentage, shown in embodiment specific as follows：
Embodiment 8
Formic acid 30%, propionic acid 30%, orthophosphoric acid 40%.
Embodiment 9
Formic acid 34%, propionic acid 26%, orthophosphoric acid 40%.
Embodiment 10
Formic acid 36%, propionic acid 30%, orthophosphoric acid 34%.
Embodiment 11
Formic acid 40%, propionic acid 24%, orthophosphoric acid 36%.
Embodiment 12
Formic acid 40%, propionic acid 20%, orthophosphoric acid 40%.
Embodiment 13
Formic acid 40%, propionic acid 30%, orthophosphoric acid 30%.
In the present invention, the acidulant is solid-state, and in the present invention, the granularity of acidulant used was that 80 mesh sieves lead to
Rate is crossed more than 80%.
A kind of feed additive for chickens based on bacillus coagulans, including the component of following weight percentage, specifically
Shown in following examples
Embodiment 14
Bacillus coagulans 4%, acidulant 2%, complex enzyme formulation 5%, carrier 89%.
Embodiment 15
Bacillus coagulans 3%, acidulant 1%, complex enzyme formulation 7%, carrier 89%.
Embodiment 16
Bacillus coagulans 2%, acidulant 5%, complex enzyme formulation 9%, carrier 84%.
Embodiment 17
Bacillus coagulans 3.5%, acidulant 1.5%, complex enzyme formulation 16%, carrier 79%.
Embodiment 18
Bacillus coagulans 8%, acidulant 4%, complex enzyme formulation 13%, carrier 75%.
Embodiment 19
Bacillus coagulans 7.5%, acidulant 4.5%, complex enzyme formulation 18%, carrier 70%.
Embodiment 20
Bacillus coagulans 10%, acidulant 5%, complex enzyme formulation 20%, carrier 65%.
Embodiment 21
Bacillus coagulans 9%, acidulant 5%, complex enzyme formulation 16%, carrier 70%.
In the present invention, the acidulant in embodiment 14 to embodiment 21 is from the above embodiments 8 into embodiment 13
The acidulant of any embodiment, the complex enzyme formulation in embodiment 14 to embodiment 21 is from the above embodiments 1 to embodiment 7
Any embodiment complex enzyme formulation, each component in embodiment 14 to embodiment 21 is well mixed obtain final product feed addition
Agent；In the present invention, the viable count of the bacillus coagulans is more than or equal to 1.0 × 1011Cfu/g less than or equal to 2.0 ×
1011Cfu/g, the bacillus coagulans is obtained by by way of solid fermentation；In the present invention, the carrier is that corn forms sediment
Powder, wheat bran or rice bran.
Test：
Test in the present invention is intended to study feed addictive to meat chicken growth performance, plant recovery of nutrient, LCFA composition
And the impact of Biochemical Indices In Serum.
Test one：Feeding experiment
1.1 feeding experiments are designed
Choose the south of the Five Ridges broiler chicken of 720 1 age in days 38.20 ± 0.07g of average weight（Purchased from the intelligence prestige agriculture of herding institute of academy of agricultural sciences of Guangdong Province
Industry Co., Ltd）, first group, second group, the 3rd group, the 4th group, the 5th group, the 6th group of totally 6 treatment groups are randomly divided into, each
6 repetitions are processed, often repeatedly 20 chickens.First group is positive control group, feeds basal diet, and second group is negative control group, is fed
Low energy daily ration, it should be strongly noted that in the present invention, low energy daily ration is low energy daily ration, low energy daily ration is basic day
Grain metabolizable energy lowers 0.42MJ/kg, and the 3rd group, the 4th group, the 5th group, the 6th group is added respectively embodiment 15 on low energy daily ration
To the feed addictive of embodiment 18.
The feeding experiment of table 1 is designed
| Treatment group | Diet Types | Addition type | Additive capacity（g/T） |
| First group（Positive control group） | Basal diet | - | - |
| Second group（Negative control group） | Low energy daily ration | - | - |
| 3rd group | Low energy daily ration | Embodiment 15 | 50 |
| 4th group | Low energy daily ration | Embodiment 16 | 75 |
| 5th group | Low energy daily ration | Embodiment 17 | 100 |
| 6th group | Low energy daily ration | Embodiment 18 | 200 |
1.2 test diet recipes and trophic level
Basal diet in test uses corn-soybean meal, with reference to meat hen nutrition requirements（NY/T 33-2004）
Prepare, test materials are powder, and basal diet diet and low energy Diet Formula and trophic level see the table below 2,3.
The basal diet formula of table 2 and trophic level（%）
Note：（1）Crude protein, crude fat, calcium and total phosphorus are measured value, and remaining is calculated value.
The present invention is the composition of first premix is specific as follows：In first premix in different days stage into
Divide and the ratio of each composition is different, 1 ~ 21 age in days：Multidimensional 0.03%, sodium bicarbonate 0.1%, fowl ore deposit 0.12%, choline 0.2% resists
Oxidant 0.015%, mould inhibitor 0.1%, zeolite powder 0.435%；22 ~ 42 ages in days：Multidimensional 0.03%, sodium bicarbonate 0.1%, fowl ore deposit
0.12%, choline 0.15%, antioxidant 0.015%, mould inhibitor 0.1%, zeolite powder 0.485%；43 ~ 63 ages in days：Multidimensional 0.03%,
Sodium bicarbonate 0.1%, fowl ore deposit 0.1%, choline 0.1%, antioxidant 0.015%, mould inhibitor 0.1%, zeolite powder 0.555%.
The low energy Diet Formula of table 3 and trophic level（%）
Note：（2）Crude protein, crude fat, calcium and total phosphorus are measured value, and remaining is calculated value.
In the present invention, the composition of second premix is specific as follows：In second premix in different days stage into
Divide and the ratio of each composition is different, 1 ~ 21 age in days %：Multidimensional 0.03%, sodium bicarbonate 0.1%, fowl ore deposit 0.12%, choline 0.2% resists
Oxidant 0.015%, mould inhibitor 0.1%, zeolite powder 0.435%；22 ~ 42 age in days %：Multidimensional 0.03%, sodium bicarbonate 0.1%, fowl ore deposit
0.12%, choline 0.15%, antioxidant 0.015%, mould inhibitor 0.1%, zeolite powder 0.485%；43 ~ 63 ages in days：Multidimensional 0.03% is little
Soda 0.1%, fowl ore deposit 0.1%, choline 0.1%, antioxidant 0.015%, mould inhibitor 0.1%, zeolite powder 0.555%.
1.3 feeding and management
Raising is divided into three phases, and 1 ~ 21 age in days is the chicken stage, and 22 ~ 42 ages in days are the middle chicken stage, and 43 ~ 63 ages in days are big chicken rank
Section, totally 63 days experimental period.Test adopts cage, free choice feeding and drinking-water, continuous illumination in 24 hours, immunity and feeding and management according to
The normal management program of chicken house is operated.
1.4 testing indexs and method
（1）Average daily gain（ADG）
Weighed in units of repetition during on-test, then respectively after chicken, middle chicken, big chicken stage terminate, to repeat
Weigh for unit, 12h fractures before weighing are not cut off the water, calculate each average daily gain for repeating every chicken.
Average daily gain（ADG）=（Test end weight-test initial weight）/ test number of days
（2）Average daily gain（ADFI）
From on-test when being repeated as the daily inventory of unit record, surplus doses and test chicken number of elements, remain doses with the
Remaining feed in hopper before two days feedings, calculates each average daily gain for repeating every chicken.As occurred in process of the test
Test chicken death, in time the dead number of elements of record, age in days, the body weight of dead chicken, deduct feed consumption rate.
Average daily gain（ADFI）=（Total amount of feeding-death chicken feed consumption rate-always remain doses）/（Remaining chicken number × number of days）
（3）Feed-weight ratio（FCR）
Feed-weight ratio（FCR）Average daily gain during average daily gain/test during=test
Result of the test：
Impact of the feed addictive of table 4 to 43 ~ 63 Day-old Broiler Chickens growth performances（n=6）
Note：Data are mean value ± standard error in table（n=6）, the different letter representation significant differences of same column shoulder note (P<0.05), contain
Same letter person represents that difference is not notable（P>0.05）.
Test result analysis：
As can be known from Table 4：
（1）First group is compared with positive control group, with the addition of feed addictive the 3rd group of test group, the 4th group, the 5th group, the 6th
Group average daily gain improves 1.36%, 1.61%, 2.06%, 3.79%, and difference is not notable（P＞ 0.05）, wherein test group the 6th
Group improves highest, and compared with basal diet, the low energy daily ration that with the addition of feed addictive is improved and adopts broiler chicken average day for this explanation
The trend of appetite.
（2）First group is compared with positive control group, the 3rd group of test group, the 4th group, the 5th group, the 6th group of average daily gain point
It is indescribably high by 2.42%, 3.33%, 2.00%, 5.15%, wherein the 6th group of highest, difference is not notable（P＞ 0.05）, it is improved average day
The trend of weightening.
（3）Second group is compared with negative control group, the 3rd group of test group, the 4th group, the 5th group, the 6th group of average daily gain point
It is indescribably high by 5.77%, 6.71%, 5.34%, 8.59%, significant difference（P＜ 0.05）, this illustrates that adding feed in low energy daily ration adds
Plus agent is remarkably improved broiler chicken average daily gain.
（4）First group is compared with positive control group, the 3rd group of test group, the 4th group, the 5th group, the 6th group of average feed-weight ratio point
Not Jiang Di by 1.08%, 1.81%, it is constant, 1.44%；Second group is compared with negative control group, the 3rd group of test group, the 4th group, the 5th group,
6th group of average feed-weight ratio reduces respectively 1.44%, 2.16%, 0.36%, 1.80%, and difference is not notable（P>0.05）But, there is drop
Low trend, illustrates that the low energy daily ration that feed addictive is with the addition of compared with basal diet has the trend for reducing feed-weight ratio.
（5）First group is compared with positive control group, the 3rd group of test group, the 4th group, the 5th group, the 6th group of punishment in advance rate drop respectively
It is low by 46.28%, 52.79%, 56.38%, 44.28%, equal significant difference（P＜ 0.05）, each group difference is notable（P>0.05）；With
Second group of negative control group compares, the 3rd group of test group, the 4th group, the 5th group, the 6th group of punishment in advance rate reduce by 42.78% respectively,
49.72%th, 53.54%, 40.65%, equal significant difference（P＜ 0.05）, each group difference is notable（P>0.05）.
In summary, in broiler chicken low energy daily ration after feed addictive of the addition containing bacillus coagulans, with low energy daily ration
Compare with basal diet, daily gain and daily ingestion amount are significantly improved, and feed-weight ratio reduces obvious.It can be seen that, addition is containing condensation
After the feed addictive of bacillus, broiler chicken feed intake increases, and abilities of digestive and absorption strengthens, and growth performance is improved.Analysis
Reason for that is after bacillus coagulans is acidified agent and carrier is wrapped, after it is entered in broiler chicken gastric juice, by hydrochloric acid in gastric juice
Corrode and damp impacts, its activity is relatively stable, in Intestine of Broiler after can play its regulating intestinal canal microorganism species
The effect of balance, suppresses the growth of harmful bacteria, promotes digesting and assimilating for nutriment, improves disease " punishment in advance " phenomenon, reduces
Just with water just, meat chicken growth performance is improved feed.Meanwhile, being acidified complex enzyme formulation that agent wraps and emulsifying agent can carry
The utilization rate of grease type energy matter in high low energy daily ration, it is to avoid oil substances are existing because broiler chicken " punishment in advance " occurs in indigestion
As, the incidence of trophism diarrhea is reduced, improve cultivation comprehensive benefit.So, the feed addictive in the present invention can reach
Improve intestine microenvironment, improve diarrhoea with dislocation of bacillary groups, reduce " punishment in advance " phenomenon, improve efficiency of feed utilization, improve cultivation
Comprehensive benefit.
Test two：Metabolic test
2.1 experimental animals and management
The close south of the Five Ridges broiler chicken of the health of 108 1 ages in days of selection, body weight carries out conventinal breeding, in 14 age in days, is randomly divided into 6
Individual treatment group, respectively the 7th group, the 8th group, the 9th group, the tenth group, the 11st group, the 12nd group, 6 weights of each treatment group
It is multiple, repeat chicken number by stage point, first stage, 3 chickens；Second stage, 2 chickens；Phase III, 1 chicken, each repeats one
Individual metabolic cage.Test chicken is placed in metabolic cage, free water and feeding, illumination in 24 hours.
2.2 experimental designs and Diet Formula
Experimental design such as table 1, whole experimental period, is divided into three phases, and 15 ~ 19 ages in days are the first stage, 36 ~ 40 ages in days are second
Stage, 57 ~ 61 ages in days are the phase III, and period feeding is added with 0.5%TiO2Corresponding daily ration, other time feeding daily ration it is same
Basal diet in table two, such as table 2.
2.3 test method
Whole experimental period, is divided into the pre- feeding phase and is just raising the phase, common 5d.It is pre- to raise phase 3d, pre- feeding is started in 15 age in days and is added with 0.5%
TiO2Diet；The d of phase 2 is just being tried, is starting to receive excrement when 18 age in days, continuously collected 2 days, 10 points of every morning and afternoon 8 are checking and accepting excrement,
Laboratory technician visually observes the state and outward appearance of excrement, and records.Broiler chicken excrement is divided into three below state, be respectively it is good, qualified,
And it is unqualified；Well：Broiler chicken excrement be dried, it is substantially anhydrous just with feed just；It is qualified：Small part broiler chicken excrement occur water just and
Feed is just；It is unqualified：Most of broiler chicken excrement there is water just or feed just.
Then, feather and scurf are removed to the excrement of above-mentioned collection, the hydrochloric acid by the every 100 g sprinklings 10% of the weight for receiving excrement is molten
The mL of liquid 5.The excrement sample collected by 2 days is placed in into 65 DEG C of baking ovens in units of repetition to dry to constant weight, get damp again 24h under room temperature, crushed
40 mesh sieves, make air-drying sample in -20 DEG C of Refrigerator stores dry to be measured, crude protein, crude fat and energy.Second stage and the 3rd
Metabolic test stage, the test method phase same first stage.
2.4 testing indexs and method
Dry is determined with reference to the method for GB/T 6435-2006 in feed and excrement；Crude protein content is used
FOSSKjeltec2300 full-automatic Kjeldahl determination devices are determined；Crude fat content is surveyed using the fine inspection SZC-C types Milko-Tester in Shanghai
It is fixed；Energy is determined using IKAC2000 calorimeters.
Apparent metabolizable energy AME（MJ/kg）=feed total energy × feed total energy digestibility
Record excrement state is in such as table 5 below：
The each group excrement state of table 5
| Group | 7th group | 8th group | 9th group | Tenth group | 11st group | 12nd group |
| Excrement state | It is unqualified | It is unqualified | It is qualified | Well | Well | Well |
Test result analysis：
From above-mentioned table 5, control group is the 7th group and the 8th group, and excrement is unqualified, i.e., still have water just with feed just phenomenon；Add
Plus the feed addictive group containing bacillus coagulans, i.e., the 9th group, the tenth group, the 11st group, the 12nd group, the state of excrement is all
It is more than qualified, wherein the tenth group, the 11st group, the 12nd group excrement is in good condition；It can be seen that, addition contains bacillus coagulans
Feed addictive after, just just phenomenon can be improved broiler chicken water with feed.
Impact of the feed addictive of table 6 to 61 Day-old Broiler Chickens plant recovery of nutrient（%, n=6）
Note：Data are mean value ± standard error in table（n=6）, the different letter representation significant differences of same column shoulder note (P<0.05), contain
Same letter person represents that difference is not notable（P>0.05）.
Test result analysis：
As known from Table 6
（1）The 8th group is compared with negative control group, the 9th group of test group, the tenth group, the 11st group, the 12nd group of dry utilization rate
It is respectively increased 2.90%, 4.45%, 3.40%, 4.18%, significant difference (P<0.05), this explanation is added feed in low energy daily ration and is added
Plus it is remarkably improved dry digestibility after agent.The 7th group is compared with positive control group, the 9th group of test group, the tenth group, the 11st
Group, the 12nd group of dry utilization rate are respectively increased 3.71%, 5.27%, 4.21%, 5.00%, significant difference (P<0.05), this says
It is bright to add after feed addictive in low energy daily ration, compare with basal diet, it is remarkably improved dry utilization rate.
（2）The 8th group is compared with negative control group, the 9th group of test group, the tenth group, the 11st group, the 12nd group of crude protein profit
Be respectively increased 4.58%, 5.06%, 3.84%, 3.23% with rate, difference not significantly (P＞ 0.05), but can be seen that in low energy daily ration
The trend of crude protein utilization rate is improved after middle addition feed addictive.The 7th group is compared with positive control group, the 9th group of test group,
Tenth group, the 11st group, the 12nd group of crude protein utilization rate be respectively increased 3.95%, 4.42%, 3.21%, 2.60%, difference does not show
Write (P＞ 0.05), but can illustrate to add after feed addictive in low energy daily ration, compare with basal diet, it is improved thick egg
The trend of white utilization rate.
（3）The 8th group is compared with negative control group, the 9th group of test group, the tenth group, the 11st group, the 12nd group of crude fat profit
4.06%, 3.15%, 4.17%, 6.14% is respectively increased with rate, wherein the 12nd group of crude fat utilization rate highest, significant difference (P＜
0.05), each group difference not significantly (P＞ 0.05)；The 7th group is compared with positive control group, the 9th group of test group, the tenth group, the tenth
One group, the 12nd group of crude fat utilization rate be respectively increased 1.51%, 0.62%, 1.62%, 3.54%, difference not significantly (P＞ 0.05),
Data above illustrates to add after feed addictive in low energy daily ration, compares with basal diet, is improved crude fat utilization rate
Trend.
（4）Relatively each group total energy data, is totally maintained in the range of 74.0%-77.0% and changes, and adds in low energy daily ration
Plus feed addictive can improve total energy utilization rate, but difference not significantly (P＞ 0.05).The 7th group is compared with positive control group, test
The 9th group of group, the tenth group, the 11st group, to be respectively increased 1.16%, 1.91%, 2.28%, 1.55% poor for the 12nd group of total energy utilization rate
It is different not notable（P>0.05）, total energy utilization rate trend is improved on the whole.
（5）Relatively each group of metabolizable energy data, are maintained in the range of 12.0-13.0MJ/kg and change little, in low energy daily ration
Middle addition feed addictive can by a small margin improve Apparent metabolizable energy（AME）, on the whole difference not significantly (P＞ 0.05).With base
Plinth daily ration is contrasted, and feed addictive is added in low energy daily ration and is improved Apparent metabolizable energy（AME）Trend.
In summary, in the present invention, in low energy daily ration after feed addictive of the addition containing bacillus coagulans, can be notable
Dry utilization rate is improved, while being improved the trend of crude protein utilization rate and total energy utilization rate.It can be seen that, the feed in the present invention
Additive can effectively improve absorption of the broiler chicken to nutriment, so as to improve the utilization rate of feed, can not only improve growth of meat chicken
Performance, while reaching the purpose for improving culture benefit.
Test three：LCFA composition measuring and method in chyme
3.1 experimental design
Experimental design with table 1, each group be designated as successively respectively the 13rd group, the 14th group, the 15th group, the 16th group, the 17th
Group, the 18th group.
3.2 sample collection
In 43 age in days, each treatment group selects at random inapparent 12 broiler chicken of weight differences, and cervical vertebra takes off after arteria carotis bloodletting
Mortar is put to death, and anatomical isolation small intestine ileal segment is sub-packed in each chicken ileum chyme in 10mL centrifuge tubes, -20 DEG C of preservations.
3.3 assay method
According to GB/T 5009.6-2003《The measure of fat in food》With GB/T 17376-2008《Animal and plant fat fat
It is prepared by sour methyl esters》In method carry out extraction of lipid and ester-interchange method esterification, last Aspirate supernatant, by 0.22 μm of filter
During dried clean sample bottle is injected after membrane filtration, it is stored in refrigerator cold storage room and waits machine analysis on GC-MS.
GC conditions：M × 0.25 mm the I.D. of DB-5 chromatographic columns 30, apply 0.25 μm of thickness (Agilent companies, U.S.
State)；Carrier gas：Nitrogen (99.999%)；260 DEG C of injector temperature, detector temperature：250℃；Split sampling mode, split ratio is 1：
30；Sample size is 1 μ L；Flow rate of carrier gas 15psi；Temperature programming：120 DEG C of starting, keeps 1 min, and with 10 DEG C/min 220 are warming up to
DEG C, 1 min is kept, 250 DEG C are warming up to 4 DEG C/min, keep 5min.
Mass Spectrometry Conditions：Ionized using electron bombardment mode, EI ion guns, electron energy 70eV, electron multiplier
Voltage 0.97kV, ion source temperature is 230 DEG C, and interface temperature is 280 DEG C.Sweep speed scope is 40 ~ 330m/z, and solvent prolongs
Late the time is 5min, and Mass Spectrometer Method plays the survey time for 3.5min.
Test result analysis：
Impact of the feed addictive of table 7 to 43 Day-old Broiler Chickens ileum LCFAs（%, n=6）
Note：Data are mean value ± standard error in table（n=6）, the different letter representation significant differences of colleague's shoulder note (P<0.05), contain
Same letter person represents that difference is not notable（P>0.05）.
As seen from the results in Table 7,
（1）The 13rd group is compared with positive control group, and respectively composition contains the 14th group of broiler chicken ileum chyme LCFA of negative control group
Amount is without significant difference（P>0.05）.
（2）Add after feed addictive to C14 in broiler chicken ileum chyme in low energy daily ration：0 and C16：1 content affects not show
Write（P>0.05）.
（3）Add after different content feed addictive in low energy daily ration, reduce C18 in chyme：2 contents, wherein locating
Manage the 16th group and reach the level of signifiance（P<0.05）, than negative control group, the 14th group reduces 12.13%；Compare negative control group
14 groups, process the 16th group and significantly reduce C16 in chyme：0 and C18：1 content（P<0.05）, reduce respectively
11.15%th, 10.56%, the 14th group of difference of other treatment groups and negative control group is not notable（P>0.05）.
（4）The 14th group of negative control group is compared, the 15th group, the 16th group, the 17th group, the 18th group for the treatment of group is tested
To C18 in chyme：0 content affects not notable（P>0.05）, C18 in processing the 15th group：0 content is minimum, processes the 17th
Group is taken second place, content highest in processing the 16th group, and the 14th group than negative control group of the 15th group of process reduces 15.07%, significantly
Less than the 16th group, the 18th group for the treatment of group（P<0.05）.
（5）The 14th group of negative control group is compared, the 18th group for the treatment of group significantly improves C20 in chyme：1 and C20：0
Content（P<0.05）, process the 16th group and significantly reduce C20 in chyme：1 and C20：0 content（P<0.05）, other add place
The 14th group of difference of reason group and negative control group is not notable（P>0.05）, wherein content is minimum in processing the 16th group, compare negative control
The 14th group of group reduces respectively 18.42%, 16.67%.
（6）Add feed addictive in daily ration affects not notable to saturated fatty acid content in broiler chicken ileum chyme（P>
0.05）, wherein content is minimum in processing the 15th group, than negative control group, the 14th group reduces 6.13%.
（7）Compare the 14th group of negative control group, process the 16th group and significantly reduce monounsaturated fatty acids in chyme, many
The content of unrighted acid, unrighted acid and TFA（P<0.05）, reduce 10.18% respectively, 10.58%,
10.45%th, 6.90%, the 14th group of difference of other treatment groups and negative control group is not notable（P>0.05）.
Test four：Biochemical Indices In Serum is determined and method
4.1 experimental design
, with table 1, feeding and management is with test one for experimental design.Each group is designated as successively respectively：19th group, the 20th group, the 20th
One group, the 22nd group, the 23rd group, the 24th group.
4.2 sample collection
In 43 age in days, each treatment group selects at random inapparent 12 broiler chicken of weight differences, and vein is taken a blood sample on an empty stomach under the wing, blood
Liquid stands in the cool a period of time after blood clotting separates out serum, and 3500r/min is centrifuged 10 min, draws upper serum point
Loaded in centrifuge tube, -30 DEG C of preservations are to be measured.
4.3 assay method
（1）Triglycerides（TG）Measuring principle：Triglycerides in Serum is set to be hydrolyzed to glycerine and aliphatic acid with lipoproteinesterase, will
The glycerine of generation, with glyceridase and atriphos phosphorylation, produces biphosphate acetone and hydrogen peroxide, generates red quinone
Imines pigment, quinone imines absorption maximum left and right at 546nm, absorbance is directly proportional to the content of triglycerides in sample.
Assay method：The kit of Bioengineering Research Institute's production is built up according to Nanjing（Enzyme is coupled colorimetric method）Determine.
Computing formula：
Content of triglyceride（mmol/L）=× concentration of standard solution（2.26mmol/L）
（2）T-CHOL（TC）Measuring principle：The cholesterol of esterification is Jing after enzyme hydrolysis, and Jing cholesterol oxidation oxydasises are produced
H2O2Aubergine pigment is generated with 4-AA and ESBmT reactions, shade is directly proportional to cholesterol level.
Assay method：The kit of Bioengineering Research Institute's production is built up according to Nanjing（COD-PAP）Determine.
Computing formula：
Total cholesterol level（mmol/L）=× concentration of standard solution（5.17mmol/L）
（3）Total protein（TP）Measuring principle：Protein molecule has-NH3+Group, when henna Coomassie brilliant blue developer
When adding in protein standard liquid or sample, the anion on coomassie stain and protein molecular-NH3+With reference to becoming solution
For blueness.
Assay method：The kit of Bioengineering Research Institute's production is built up according to Nanjing（Coomassie brilliant blue）Determine.
Computing formula：
Protein concentration（g/L）=× standard pipe concentration（0.563g/L）× extension rate
（4）Free fatty（NEFA）Measuring principle：Free fatty can combine to form the mantoquita of aliphatic acid and molten with copper ion
In chloroform, its content is directly proportional to free fatty acid content.The content of wherein copper ion is determined with DDTC, you can extrapolate
The content of free fatty.
Assay method：The kit measurement of Bioengineering Research Institute's production is built up according to Nanjing.
Computing formula：Serum N EFA content（mmol/L）=× concentration of standard solution（1mmol/L）
4.4 result of the test
Impact of the feed addictive of table 8 to 43 Day-old Broiler Chickens Biochemical Indices In Serums（n=6）
Note：Data are mean value ± standard error in table（n=6）, the different letter representation significant differences of same column shoulder note (P<0.05), contain
Same letter person represents that difference is not notable（P>0.05）.
As can be known from Table 8：
（1）The 19th group is compared with positive control group, the 20th group of triglycerides of negative control group, T-CHOL, total protein and free
Content of fatty acid does not have significant difference（P>0.05）.
（2）With the 20th group of negative control group, add in low energy daily ration after various dose feed addictive and increased glycerine
The content of three esters, is respectively increased 28.33%, 18.03%, 21.46%, 14.59%, significant difference（P＜ 0.05）, wherein processing second
11 content highests, the 22nd content of process is minimum, and each group difference is not notable（P>0.05）.With the 19th group of positive control group
Relatively, content of triglyceride after various dose feed addictive is added in low energy daily ration to be increased, increase by 48.02% respectively,
36.14%th, 40.10%, 32.18%, significant difference（P＜ 0.05）, each group difference is notable（P>0.05）.
（3）With the 20th group of negative control group, various dose feed addictive is added in low energy daily ration total courage in serum is consolidated
Alcohol content affects not notable（P>0.05）, wherein processing, the 24th group of content is minimum, than negative control group, the 20th group reduces
5.41%.The 19th group is compared with positive control group, and T-CHOL after various dose feed addictive is added in low energy daily ration to be had substantially
The trend of reduction, reduces respectively 10.04%, 8.24%, 6.81%, 12.19%, and difference is not notable（P>0.05）.
（4）The 20th group of negative control group is compared, various dose feed addictive is added in low energy daily ration reduction total protein
The trend of content, the 22nd group of content of process is minimum, and each treatment group is shown in that difference is not notable（P>0.05）.With positive control group the tenth
Nine groups are compared, the 21st group for the treatment of group, the 22nd group, the 23rd group, the 24th group of total protein reduce respectively
14.56%th, 15.71%, 15.21%, 15.39%, reduce trend obvious.
（5）The 20th group of negative control group is compared, the 21st group of process, the 23rd group of free fatty acid content have reduction
Trend, reduces respectively 15.87%, 26.98%, and difference is not notable（P>0.05）, and process the 22nd group, the 24th group of difference
50.79%, 31.75% is reduced, significant difference（P<0.05）；Compare with positive control group, the 21st group for the treatment of group, the 23rd
Group, the 24th group of free fatty acid content reduce respectively 11.67%, 23.33%, 28.33%, and difference is not notable（P>0.05）, the
22 groups of reductions by 48.33%, significant difference（P<0.05）.
In sum, adding in low energy daily ration can significantly improve content of triglyceride after feed addictive, reduction cholesterol,
Total protein and free fatty acid content.It can be seen that, the feed addictive in the present invention can improve content of triglyceride in Intestine of Broiler,
Cholesterol level is reduced, while the absorption to total protein and free fatty has obvious facilitation, it is possible to increase nutrition
Material utilization, improves Biochemical Indices In Serum.
In the present invention, also including the emulsifying agent that percentage by weight is 2%-10%.Emulsifying agent in the present invention is soybean lecithin
Can also be lecithin or cephalin etc., emulsifying agent also known as detergent or surfactant, by hydrophilic group and hydrophobic group two
It is grouped into, is that a class has and promotes that two or more immiscible liquid is homodisperse a kind of to live with typical surface
Property effect material, have the advantages that emulsified fat, promotion digest and assimilate, broiler chicken feeding after can promote growing for broiler chicken,
Efficiency of feed utilization is improved, growth performance is improved, the young age livestock and poultry shortcoming not high to Lipid use rate in feed is overcome.With the addition of
The embodiment of the feed addictive after emulsifying agent is as follows：
Embodiment 22
Bacillus coagulans 4%, acidulant 5%, complex enzyme formulation 16%, emulsifying agent 10%, carrier 65%.
Embodiment 23
Bacillus coagulans 10%, acidulant 3%, complex enzyme formulation 20%, emulsifying agent 2%, carrier 65%.
Test five：
Choose the south of the Five Ridges broiler chicken of 960 1 age in days 37.58 ± 0.05g of average weight（Purchased from the intelligence prestige agriculture of herding institute of academy of agricultural sciences of Guangdong Province
Industry Co., Ltd）, the 25th group, the 26th group, the 27th group, the 28th group of totally 4 treatment groups are randomly divided into, often
6 repetitions of individual process, often repeatedly 40 chickens.25th group is positive control group, feeds basal diet, and the 26th group is negative right
According to group, that is, low energy daily ration is fed, the 27th group, the 28th group is added respectively embodiment 22 to embodiment on low energy daily ration
23 feed addictive.
The feeding experiment of table 9 is designed
Table 10 with the addition of impact of the feed addictive of emulsifying agent to 43 ~ 63 Day-old Broiler Chickens growth performances（n=6）
The result of the test of table 10 understands that add in feed addictive after emulsifying agent, daily ingestion amount and daily gain have becoming for increase
Gesture, feedstuff-meat ratio reduces obvious.The 25th group is compared with positive control group, the 27th group of test group and the 28th group of feedstuff-meat ratio
1.47%, 2.20% is reduced, difference is not notable（P>0.05）.Emulsifying agent in the present invention can promote utilization rate of the broiler chicken to grease,
The trophism diarrhea that oil substances are caused because of indigestion is reduced, improves trophism " punishment in advance " phenomenon.
The present invention also provides a kind of broiler fodder, and the broiler fodder contains arbitrary in above-described embodiment 14 to embodiment 21
Plant any one feed addictive in feed addictive, or embodiment 22 to embodiment 23, feed addictive in the feed
Mass percent be 0.01%-0.03%.
Embodiment 24
Feed addictive 0.01%, base-material 99.9%.
Embodiment 25
Feed addictive 0.03%, base-material 99.7%.
Test six：
Choose the south of the Five Ridges broiler chicken of 360 1 age in days 37.58 ± 0.08g of average weight（Purchased from the intelligence prestige agriculture of herding institute of academy of agricultural sciences of Guangdong Province
Industry Co., Ltd）, the 29th group, the 30th group, the 31st group of totally 3 treatment groups are randomly divided into, each processes 6 weights
It is multiple, every repeatedly 20 chickens.Per group of correspondence feeds respectively the feed in feed and embodiment 25 in basal diet, embodiment 24,
Wherein the 29th group is control group, feeds basal diet.
Impact of the feed addictive of table 11 to 43 ~ 63 Day-old Broiler Chickens growth performances（n=6）
| Average daily gain（g/d） | Average daily gain（g/d） | Feed-weight ratio | |
| 29th group（Control group） | 126.3±2.0 | 45.1±0.9 | 2.80±0.04 |
| 30th group（Feeding embodiment 24） | 127.8±1.2 | 46.6±0.5 | 2.74±0.05 |
| 31st group（Feeding embodiment 25） | 128.0±1.7 | 47.0±0.8 | 2.72±0.08 |
The result of the test of table 11 understands that the 29th group is compared with control group, the 30th group of test group and the 31st group of average day
The trend that feed intake is improved, average daily gain is respectively increased 3.33%, 4.21%, and feedstuff-meat ratio reduces respectively 2.14%, 2.86%.By
This is visible, can improve broiler chicken feed intake and daily gain after feed addictive of the addition containing bacillus coagulans in daily ration of broiler, together
When feedstuff-meat ratio reduce obvious.This explanation can be effectively improved broiler chicken intestines after feed addictive of the addition containing bacillus coagulans
Road microorganism species balance, improves immunity of organisms and premunition, improves efficiency of feed utilization, and meat chicken growth performance is improved,
Improve cultivation comprehensive benefit.
In the present invention, the base-material is basal diet or low energy daily ration.
In the present invention, wherein, the basal diet in the feed includes the component of following weight percentage, specific embodiment
It is as follows：
Embodiment 26
Corn 50%, dregs of beans 20%, corn protein powder 6%, vinasse albumen feed (DDGS) 6%, level Four soybean oil 6%, wheat-middlings 5%, stone
Powder 1.6%, calcium monohydrogen phosphate 1.5%, salt 0.4%, lysine 0.5%, solid methionine 1.2%, threonine 0.8%, the first premix
1.0%。
Embodiment 27
Corn 50%, dregs of beans 30%, corn protein powder 5%, vinasse albumen feed (DDGS) 4%, level Four soybean oil 6%, wheat-middlings 0.1%,
Stone flour 1.3%, calcium monohydrogen phosphate 0.6%, salt 0.3%, lysine 0.3%, solid methionine 1%, threonine 0.4%, the first premix
1.0%。
Embodiment 28
Corn 65%, dregs of beans 15%, corn protein powder 3%, vinasse albumen feed (DDGS) 6%, level Four soybean oil 2%, wheat-middlings 4.9%,
Stone flour 1.3%, calcium monohydrogen phosphate 0.6%, salt 0.3%, lysine 0.3%, solid methionine 0.4%, threonine 0.2%, the first premix
Material 1.0%.
In the present invention, wherein, the low energy daily ration in the feed includes the component of following weight percentage, specific embodiment
It is as follows：
Embodiment 29
Corn 58.95%, dregs of beans 28%, corn protein powder 4%, vinasse albumen feed (DDGS) 5%, level Four soybean oil 0.4%, wheat-middlings
0.1%, stone flour 1.3%, calcium monohydrogen phosphate 0.6%, salt 0.3%, lysine 0.3%, solid methionine 0.03%, threonine 0.02%, the
Two premixes 1.0%.
Embodiment 30
Corn 59.82%, dregs of beans 14%, corn protein powder 5%, vinasse albumen feed (DDGS) 6%, level Four soybean oil 4%, wheat-middlings
5%, stone flour 1.5%, calcium monohydrogen phosphate 1.5%, salt 0.4%, lysine 0.5%, solid methionine 1.2%, threonine 0.08%, second
Premix 1.0%.
Embodiment 31
Corn 55%, dregs of beans 25%, corn protein powder 4%, vinasse albumen feed (DDGS) 5%, level Four soybean oil 1%, wheat-middlings 5%, stone
Powder 1.5%, calcium monohydrogen phosphate 1.5%, salt 0.4%, lysine 0.5%, solid methionine 0.05%, threonine 0.05%, the second premix
Material 1.0%.
Embodiment 32
Corn 65%, dregs of beans 15%, corn protein powder 4%, vinasse albumen feed (DDGS) 5%, level Four soybean oil 1%, wheat-middlings 5%, stone
Powder 1.5%, calcium monohydrogen phosphate 1.5%, salt 0.4%, lysine 0.5%, solid methionine 0.05%, threonine 0.05%, the second premix
Material 1.0%.
Claims (10)
1. a kind of feed additive for chickens based on bacillus coagulans, it is characterised in that：Including following weight percentage
Component：Bacillus coagulans 2%-10%, acidulant 1%-5%, complex enzyme formulation 5%-20%, carrier 65%-89%.
2. the feed additive for chickens based on bacillus coagulans according to claim 1, it is characterised in that：Including under
State the component of percentage by weight：Bacillus coagulans 4%-8%, acidulant 2%-4%, complex enzyme formulation 9%-16%, carrier 75%-
79%。
3. the feed additive for chickens based on bacillus coagulans according to claim 1 or 2, it is characterised in that：Also
Including the emulsifying agent that percentage by weight is 2%-10%.
4. the feed additive for chickens based on bacillus coagulans according to claim 1 and 2, it is characterised in that：Its
In, the complex enzyme formulation includes the component of following weight percentage：Lipase 10%-20%, zytase 10%-20%, Portugal gather
Carbohydrase 10%-20%, mannase 5%-10%, amylase 5%-10%, acid protease 5%-10%, alkali protease 10%-20%,
Galactosidase 5%-10%.
5. the feed additive for chickens based on bacillus coagulans according to claim 1 and 2, it is characterised in that：Its
In, the acidulant includes the component of following weight percentage：Formic acid 30%-40%, propionic acid 20%-30%, orthophosphoric acid 30%-40%.
6. a kind of broiler fodder, it is characterised in that：The feed contains claim 1 to any one feed described in claim 5
Additive, feed addictive 0.01%-0.03%, the 7%-99. 9% of base-material 99..
7. broiler fodder according to claim 6, it is characterised in that：The base-material is basal diet or low energy daily ration.
8. broiler fodder according to claim 7, it is characterised in that：Wherein, the basal diet includes following weight hundred
Divide the component of ratio：Corn 50%-65%, dregs of beans 15%-30%, corn protein powder 3%-6%, vinasse albumen feed (DDGS) 4%-6%, four
Level soybean oil 2%-6%, wheat-middlings 0.1%-5%, stone flour 1.3%-1.6%, calcium monohydrogen phosphate 0.6%-1.5%, salt 0.3%-0.4% rely ammonia
Sour 0.3%-0.5%, solid methionine 0.4%-1.2%, threonine 0.2%-0.8%, the first premix 1%.
9. broiler fodder according to claim 7, it is characterised in that：Wherein, the low energy daily ration includes following weight hundred
Divide the component of ratio：Corn 55%-65%, dregs of beans 14%-28%, corn protein powder 4%-5%, vinasse albumen feed (DDGS) 5%-6%, four
Level soybean oil 0.4%-4%, wheat-middlings 0.1%-5%, stone flour 1.3%-1.5%, calcium monohydrogen phosphate 0.6%-1.5%, salt 0.3%-0.4% rely
Propylhomoserin 0.3%-0.5%, solid methionine 0.03%-1.2%, threonine 0.02%-0.08%, the second premix 1%.
10. application of a kind of feed additive for chickens based on bacillus coagulans in terms of broiler fodder.
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| CN109874919A (en) * | 2019-03-05 | 2019-06-14 | 武汉新华扬生物股份有限公司 | A kind of additive agent for feeding, preparation method and applications |
| CN111004732A (en) * | 2019-03-13 | 2020-04-14 | 江南大学 | Bacillus coagulans capable of promoting motilin secretion and application thereof |
| CN113528368A (en) * | 2020-04-15 | 2021-10-22 | 青岛蔚蓝生物股份有限公司 | Bacillus coagulans preparation and preparation method thereof |
| CN113528367A (en) * | 2020-04-15 | 2021-10-22 | 青岛蔚蓝生物股份有限公司 | Bacillus coagulans with functions of preventing diarrhea and degrading cholesterol |
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| CN107356494A (en) * | 2017-08-29 | 2017-11-17 | 青岛农业大学 | Broiler fodder crosses the appraisal procedure of doses |
| CN109874919A (en) * | 2019-03-05 | 2019-06-14 | 武汉新华扬生物股份有限公司 | A kind of additive agent for feeding, preparation method and applications |
| CN111004732A (en) * | 2019-03-13 | 2020-04-14 | 江南大学 | Bacillus coagulans capable of promoting motilin secretion and application thereof |
| CN111004732B (en) * | 2019-03-13 | 2021-08-24 | 江南大学 | Bacillus coagulans capable of promoting motilin secretion and application thereof |
| CN113528368A (en) * | 2020-04-15 | 2021-10-22 | 青岛蔚蓝生物股份有限公司 | Bacillus coagulans preparation and preparation method thereof |
| CN113528367A (en) * | 2020-04-15 | 2021-10-22 | 青岛蔚蓝生物股份有限公司 | Bacillus coagulans with functions of preventing diarrhea and degrading cholesterol |
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Application publication date: 20170510 |