CN101642198A - Biological additive pre-mixed feed special for chicken and preparation method of biological additive substance carrier thereof - Google Patents
Biological additive pre-mixed feed special for chicken and preparation method of biological additive substance carrier thereof Download PDFInfo
- Publication number
- CN101642198A CN101642198A CN200910065923A CN200910065923A CN101642198A CN 101642198 A CN101642198 A CN 101642198A CN 200910065923 A CN200910065923 A CN 200910065923A CN 200910065923 A CN200910065923 A CN 200910065923A CN 101642198 A CN101642198 A CN 101642198A
- Authority
- CN
- China
- Prior art keywords
- liquid
- fermentation
- bioactivator
- preparation
- tsao
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 118
- 239000000654 additive Substances 0.000 title claims abstract description 76
- 239000000126 substance Substances 0.000 title claims abstract description 58
- 241000287828 Gallus gallus Species 0.000 title claims abstract description 43
- 230000000996 additive effect Effects 0.000 title claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 191
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 83
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 83
- 244000126002 Ziziphus vulgaris Species 0.000 claims abstract description 77
- 235000008529 Ziziphus vulgaris Nutrition 0.000 claims abstract description 77
- 102000004190 Enzymes Human genes 0.000 claims abstract description 62
- 108090000790 Enzymes Proteins 0.000 claims abstract description 62
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 26
- 235000012054 meals Nutrition 0.000 claims abstract description 25
- 150000001413 amino acids Chemical class 0.000 claims abstract description 22
- 229940088594 vitamin Drugs 0.000 claims abstract description 19
- 235000013343 vitamin Nutrition 0.000 claims abstract description 19
- 239000011782 vitamin Substances 0.000 claims abstract description 19
- 229930003231 vitamin Natural products 0.000 claims abstract description 19
- 150000003722 vitamin derivatives Chemical class 0.000 claims abstract description 19
- 239000002366 mineral element Substances 0.000 claims abstract description 16
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 claims abstract description 13
- 235000020661 alpha-linolenic acid Nutrition 0.000 claims abstract description 13
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims abstract description 13
- 229910000019 calcium carbonate Inorganic materials 0.000 claims abstract description 13
- 229910000389 calcium phosphate Inorganic materials 0.000 claims abstract description 13
- 229960004488 linolenic acid Drugs 0.000 claims abstract description 13
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 claims abstract description 13
- 235000019691 monocalcium phosphate Nutrition 0.000 claims abstract description 13
- 238000000855 fermentation Methods 0.000 claims description 182
- 230000004151 fermentation Effects 0.000 claims description 182
- 235000010469 Glycine max Nutrition 0.000 claims description 98
- 244000068988 Glycine max Species 0.000 claims description 92
- 239000000758 substrate Substances 0.000 claims description 82
- 241000894006 Bacteria Species 0.000 claims description 65
- 241000193749 Bacillus coagulans Species 0.000 claims description 60
- 229940054340 bacillus coagulans Drugs 0.000 claims description 60
- 238000004458 analytical method Methods 0.000 claims description 55
- 230000001954 sterilising effect Effects 0.000 claims description 52
- 238000004659 sterilization and disinfection Methods 0.000 claims description 52
- 230000000975 bioactive effect Effects 0.000 claims description 47
- 239000000843 powder Substances 0.000 claims description 45
- 235000013379 molasses Nutrition 0.000 claims description 36
- 235000013336 milk Nutrition 0.000 claims description 35
- 239000008267 milk Substances 0.000 claims description 35
- 210000004080 milk Anatomy 0.000 claims description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 33
- 235000015097 nutrients Nutrition 0.000 claims description 31
- 235000020183 skimmed milk Nutrition 0.000 claims description 30
- 238000010563 solid-state fermentation Methods 0.000 claims description 26
- 239000007864 aqueous solution Substances 0.000 claims description 24
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 23
- 230000001925 catabolic effect Effects 0.000 claims description 21
- 239000000463 material Substances 0.000 claims description 19
- 240000007594 Oryza sativa Species 0.000 claims description 16
- 235000007164 Oryza sativa Nutrition 0.000 claims description 16
- 239000004365 Protease Substances 0.000 claims description 16
- 235000009566 rice Nutrition 0.000 claims description 16
- 108010004032 Bromelains Proteins 0.000 claims description 15
- 102000005840 alpha-Galactosidase Human genes 0.000 claims description 15
- 108010030291 alpha-Galactosidase Proteins 0.000 claims description 15
- 235000019835 bromelain Nutrition 0.000 claims description 15
- 239000013589 supplement Substances 0.000 claims description 15
- 239000003053 toxin Substances 0.000 claims description 15
- 231100000765 toxin Toxicity 0.000 claims description 15
- 238000007796 conventional method Methods 0.000 claims description 14
- 239000002594 sorbent Substances 0.000 claims description 13
- 229940062672 calcium dihydrogen phosphate Drugs 0.000 claims description 12
- 239000002994 raw material Substances 0.000 claims description 12
- 244000099147 Ananas comosus Species 0.000 claims description 10
- 235000007119 Ananas comosus Nutrition 0.000 claims description 10
- 230000001461 cytolytic effect Effects 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 9
- 235000009508 confectionery Nutrition 0.000 claims description 6
- 239000012467 final product Substances 0.000 claims description 6
- 239000005457 ice water Substances 0.000 claims description 6
- 239000002893 slag Substances 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 5
- 240000006677 Vicia faba Species 0.000 claims description 5
- 235000010749 Vicia faba Nutrition 0.000 claims description 5
- 235000002098 Vicia faba var. major Nutrition 0.000 claims description 5
- 239000000047 product Substances 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 4
- 239000012141 concentrate Substances 0.000 claims description 4
- 235000015099 wheat brans Nutrition 0.000 claims description 4
- 229920001221 xylan Polymers 0.000 claims description 4
- 150000004823 xylans Chemical class 0.000 claims description 4
- 235000013339 cereals Nutrition 0.000 claims description 3
- 101800000263 Acidic protein Proteins 0.000 claims description 2
- 240000002791 Brassica napus Species 0.000 claims description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- 240000005979 Hordeum vulgare Species 0.000 claims description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 2
- 235000005822 corn Nutrition 0.000 claims description 2
- 235000012343 cottonseed oil Nutrition 0.000 claims description 2
- 235000013312 flour Nutrition 0.000 claims description 2
- 235000009973 maize Nutrition 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 6
- 239000007787 solid Substances 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 2
- 239000013543 active substance Substances 0.000 abstract 2
- 241001474374 Blennius Species 0.000 abstract 1
- 239000003463 adsorbent Substances 0.000 abstract 1
- 235000010216 calcium carbonate Nutrition 0.000 abstract 1
- 239000003640 drug residue Substances 0.000 abstract 1
- 230000001988 toxicity Effects 0.000 abstract 1
- 231100000419 toxicity Toxicity 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 49
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 21
- 239000012895 dilution Substances 0.000 description 20
- 238000010790 dilution Methods 0.000 description 20
- 235000001014 amino acid Nutrition 0.000 description 19
- 239000000203 mixture Substances 0.000 description 16
- 230000001580 bacterial effect Effects 0.000 description 12
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 241001465754 Metazoa Species 0.000 description 9
- 241000726221 Gemma Species 0.000 description 7
- 239000002253 acid Substances 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- 239000007853 buffer solution Substances 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 210000004243 sweat Anatomy 0.000 description 6
- 108010019160 Pancreatin Proteins 0.000 description 5
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 5
- 229910000397 disodium phosphate Inorganic materials 0.000 description 5
- 235000019800 disodium phosphate Nutrition 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 239000004310 lactic acid Substances 0.000 description 5
- 235000014655 lactic acid Nutrition 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 235000016709 nutrition Nutrition 0.000 description 5
- 229940055695 pancreatin Drugs 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 102000016938 Catalase Human genes 0.000 description 4
- 108010053835 Catalase Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 150000007513 acids Chemical class 0.000 description 4
- 150000001720 carbohydrates Chemical class 0.000 description 4
- 235000014633 carbohydrates Nutrition 0.000 description 4
- 230000009514 concussion Effects 0.000 description 4
- 238000010899 nucleation Methods 0.000 description 4
- 235000018291 probiotics Nutrition 0.000 description 4
- 238000009938 salting Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 239000011149 active material Substances 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 3
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 239000006041 probiotic Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 108010050327 trypticase-soy broth Proteins 0.000 description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 2
- 229930091371 Fructose Natural products 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000009313 farming Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000009499 grossing Methods 0.000 description 2
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 229940099596 manganese sulfate Drugs 0.000 description 2
- 239000011702 manganese sulphate Substances 0.000 description 2
- 235000007079 manganese sulphate Nutrition 0.000 description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000013028 medium composition Substances 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 238000007747 plating Methods 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 229960003487 xylose Drugs 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 102000002659 Amyloid Precursor Protein Secretases Human genes 0.000 description 1
- 108010043324 Amyloid Precursor Protein Secretases Proteins 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 1
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 108010076119 Caseins Proteins 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 235000007466 Corylus avellana Nutrition 0.000 description 1
- 240000003211 Corylus maxima Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 1
- 240000001046 Lactobacillus acidophilus Species 0.000 description 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 1
- 244000199866 Lactobacillus casei Species 0.000 description 1
- 235000013958 Lactobacillus casei Nutrition 0.000 description 1
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 229960005069 calcium Drugs 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 108010089934 carbohydrase Proteins 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229940099352 cholate Drugs 0.000 description 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
- KTVIXTQDYHMGHF-UHFFFAOYSA-L cobalt(2+) sulfate Chemical compound [Co+2].[O-]S([O-])(=O)=O KTVIXTQDYHMGHF-UHFFFAOYSA-L 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000003278 egg shell Anatomy 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 229940032049 enterococcus faecalis Drugs 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 210000004051 gastric juice Anatomy 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- WHWDWIHXSPCOKZ-UHFFFAOYSA-N hexahydrofarnesyl acetone Natural products CC(C)CCCC(C)CCCC(C)CCCC(C)=O WHWDWIHXSPCOKZ-UHFFFAOYSA-N 0.000 description 1
- 239000008240 homogeneous mixture Substances 0.000 description 1
- 230000008799 immune stress Effects 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000031891 intestinal absorption Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 229940017800 lactobacillus casei Drugs 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000008557 oxygen metabolism Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 230000036642 wellbeing Effects 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses biological additive pre-mixed feed special for chicken and a preparation method of a biological additive substance carrier thereof. The biological additive pre-mixed feed special for chicken comprises the biological additive substance carrier, monobasic calcium phosphate, calcium carbonate, vitamin additives, amino acid additives, mineral elements, an enzyme preparation, amycotoxin adsorbent, a linolenic acid, a liquid biological active substance containing 20-45 percent of dry bacillus subtilis Tsao 060605 and a liquid biological active substance containing 20-45 percent of condensed bacillus Leehai 887, wherein the biological additive substance carrier is prepared by liquid zymotic biological additive substances and solid zymotic biological additive substances containing the bacillus subtilis Tsao 060605 and the condensed bacillus Leehai 887 as well as added seaweed meal. The novel biological additive pre-mixed feed special for chicken, which is prepared by the method, has low cost and environmental protection without side effect, residue, drug fastness or toxicity.
Description
One, technical field:
The present invention relates to a kind of chicken special feed, particularly relate to the preparation method of a kind of chicken bio-additive premixed feed special and bioactive substance carrier thereof.
Two, background technology:
Much more general the feeding of economic animal raised the material that possesses nutritional need, and just so-called feed can promote growth, production and the health of animal like this, and is safe and effective to animal under rationally using.In order to satisfy specific demand, and add a small amount of or micro-material in feed processing, making and use, these materials generally include enzyme preparation, probio, probiotic, Amino acid, vitamin, trace quantity mineral substance element etc.Bio-additive premixed feed then is the homogeneous mixture of being prepared by a certain percentage by two or more probio or prebiotics feed additive and carrier or diluent, has comprised Amino acid, vitamin, trace quantity mineral substance element simultaneously.
At present, for fear of in animal feed, adding the toxic and side effect that antibiotic brought.Animal farming industry is taked to replace antibiotic additive and is embraced a wide spectrum of ideas in feed, wherein common with the research and development of probio, saccharomycete, acidulant, enzyme preparation and Chinese herbal and crude drugs preparations, the research of this aspect is the most deeply concerned and maximum project of injecting capital into of modern biotech industry and farming animals aquaculture industry.Probio is exactly a microorganism formulation, can be described as bacteria-promoting agent again, be widely used abroad, 1989 FDA (FDA) announced and can directly feed and be commonly considered as safe microbial species list, have 41 kinds, comprise bacillus licheniformis, bacillus subtilis, bacillus coagulans, bifidobacterium, thermophilic streptococcus, enterococcus faecalis, lactoenterococcus, lactobacillus acidophilus, Lactobacillus casei, lactobacillus lactis, lactobacillus plantarum, saccharomyces cerevisiae and subspecies thereof or make a variation and plant or the like.
Using probio to cooperate natural fermented matrix is the suitable selection of producing environment-protecting feed, accurate allotment carbon/nitrogen ratio, unit interval dissolved oxygen amount, pH-value, temperature, time and special interpolation material, the mat bacterial classification is removed nutrition inhibiting factor or toxin to the transformation of organic matter, the big molecule of degrading becomes small-molecular peptides, and nutriment is more conducive to by the animal intestinal absorption; The probio reproductive process also can produce various metabolites with lifting immunity and comprise acidulant, various digestive ferments such as Cobastab group, nucleotides, Amino acid and lactic acid, acetic acid, promote the few candy of fruit, the butyrate of intestinal tract normal flora clump balance in addition, these water soluble metaboliteses add that active probiotic will be a carrier with natural fermented matrix all, do accurate ratio allotment with Amino acid, vitamin, trace quantity mineral substance element again, develop into bio-additive premixed feed as theoretical foundation.
Three, summary of the invention:
The technical problem to be solved in the present invention is: the preparation method that a kind of chicken bio-additive premixed feed special and bioactive substance carrier thereof are provided.Utilize the chicken bio-additive premixed feed special of technical solution of the present invention preparation can promote the feed nutrition transformation efficiency significantly, promote chicken immunity, reduce stress effect, promote the economic well-being of workers and staff of culturing the peasant.
Technical scheme of the present invention is:
The invention provides a kind of preparation method of bioactive substance carrier, described bioactive substance carrier is the solid state fermentation active material that is contained seawood meal by bacillus subtilis Tsao 060605 and bacillus coagulans Leehai 887 composite fermentations, add a kind of bioactive substance carrier that the liquid fermenting organism active material of two kinds of bacterial classifications is comprehensive and prepare, this preparation method's detailed step is as follows:
A, the preparation of bacillus subtilis Tsao 060605 liquid bioactivator: earlier molasses or soya-bean milk are diluted to sterilized water that to measure concentration with balling hydrometer be 1～7brix, under 100 ℃ of conditions, after the sterilization treatment 12～18 minutes, get 3500～4200L and put into fermentation tank, the bacillus subtilis Tsao 060605 bacterium liquid 500L that initial stage is cultivated through conventional method pours fermentation tank into again, and then in sterilization tank, being that the analysis for soybean powder nutrient source aqueous solution of 7% skimmed milk power or 5% is with 100 ℃ with the concentration of 1000L sterilized water preparation, after 12～18 minutes sterilization treatment, get 300～1000L and put into fermentation tank, make described skimmed milk power or analysis for soybean powder be respectively 0.6～2.1% or 0.4～1.5% with respect to molasses or its concentration of soya-bean milk, the described concentration that contains in 5% the analysis for soybean powder aqueous solution with sterilized water preparation in sterilization tank is 0.001% pineapple ferment, comprehensive above nutrient source prepares liquid substrate and ferments, in the fermentation cylinder for fermentation temperature is 27～55 ℃, fermentation time is 10～48 hours, when stopping, fermentation adds catabolic enzyme, its addition is to account for 0.01～0.5% of solid-state substrate total amount described in the step c, when adding catabolic enzyme, with ice water circulation the zymocyte liquid temperature is reduced to 7～8 ℃ in the fermentation tank chuck and get final product;
B, the preparation of bacillus coagulans Leehai 887 liquid bioactivators: earlier molasses or soya-bean milk are diluted to sterilized water that to measure concentration with balling hydrometer be 1.5～9brix, under 100 ℃ of conditions, after the sterilization treatment 12～18 minutes, get 3500～4200L and put into fermentation tank, the bacillus coagulans Leehai 887 bacterium liquid 500L that initial stage is cultivated through conventional method pour fermentation tank into again, again in sterilization tank, being the analysis for soybean powder of 8% skimmed milk power or 7% and 1% the anhydrous phosphoric acid dipotassium nutrient source aqueous solution with the concentration of 1000L sterilized water preparation, with 100 ℃, get 300～1000L after 12～18 minutes sterilization treatment and put into fermentation tank, make described skimmed milk power or analysis for soybean powder and anhydrous phosphoric acid dipotassium be respectively 0.7～2.4% or 0.6～2.1% and 0.08～0.3% with respect to molasses or its concentration of soya-bean milk, the described concentration that contains in 7% the analysis for soybean powder aqueous solution with sterilized water preparation in sterilization tank is 0.001% pineapple ferment, comprehensive above nutrient source prepares liquid substrate and ferments, fermentation temperature is 37～42 ℃ during fermentation, fermentation time is 18～48 hours, when stopping, fermentation adds bromelain and alpha-galactosidase, the addition of described bromelain accounts for 0.0005～0.01% of solid-state substrate total amount described in the step c, the addition of described alpha-galactosidase accounts for 0.005～0.05% of solid-state substrate total amount described in the step c, when adding enzyme, with ice water circulation the zymocyte liquid temperature is reduced to 7～8 ℃ in the fermentation tank chuck and get final product;
C, the preparation of bacillus subtilis Tsao 060605 and bacillus coagulans Leehai 887 solid-state bioactivators: earlier with solid-state substrate modified 5～30 minutes with steam, be cooled to 40～43 ℃, then the bacillus subtilis Tsao 060605 liquid bioactivator of step a preparation and the bacillus coagulans Leehai 887 liquid bioactivators of step b preparation are mixed, the shared weight percentage of the liquid bioactivator of bacillus subtilis Tsao060605 is 10～30% during mixing, solid-state substrate and mixed liquid bioactivator after handling are carried out mixed culture fermentation, when solid-state substrate mixes with mixed liquid bioactivator, the shared weight percentage of wherein mixed liquid bioactivator is 25～95%, fermentation temperature is 30～55 ℃ during fermentation, fermentation time is 12～72 hours, finish preceding 0～8 hour adding seawood meal at solid state fermentation, the addition of described seawood meal accounts for 1～10% of solid-state substrate total amount, after the fermentation ends its material is dried under 55～100 ℃ temperature, dry to fermentation substrate moisture≤7%, pulverize at last, obtain the solid state fermentation bioactivator;
D, the preparation of bioactive substance carrier: with step a, it is 20～45% liquid state fermentation bioactivator that the bacillus subtilis Tsao 060605 liquid bioactivator of b preparation and bacillus coagulans Leehai 887 liquid bioactivators are condensed into the dry amount respectively, liquid state fermentation bioactivator after two kinds concentrated mixes, wherein the dry amount is that 20～45% the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator is 40～50%, the dry amount is that 20～45% the shared weight percentage of bacillus coagulans Leehai 887 liquid bioactivators is 50～60%, mixed liquid state fermentation bioactivator is sprayed on the solid state fermentation bioactivator for preparing among the step c, described mixed liquid state fermentation bioactivator sprinkling amount accounts for 0.5～1.5% of solid state fermentation bioactivator total amount, promptly gets bioactive substance carrier after the sprinkling.
According to the preparation method of above-mentioned bioactive substance carrier, the bacterium number in the bacillus subtilis Tsao 060605 bacterium liquid that conventional method is cultivated of initial stage described in the step a is 〉=1.0 * 10
8CFU/ml, pH-value pH are 5.8～6.5; The bacterium number is 〉=1.2 * 10 in the bacillus subtilis Tsao 060605 bacterium liquid of described fermentation back
7CFU/ml.PH-value pH is 5.8～6.5.
According to the preparation method of above-mentioned bioactive substance carrier, the granularity of analysis for soybean powder described in the step a is all to sieve by 40～60 mesh sieve analyses; Described catabolic enzyme is at least a in the poly-candy enzyme of acidic protein catabolic enzyme, bromelain, alphalise starch catabolic enzyme, cellulolytic enzyme, half cellulolytic enzyme, alpha-galactosidase, Xylans enzyme, sweet dew.
According to the preparation method of above-mentioned bioactive substance carrier, the bacterium number in the bacillus coagulans Leehai 887 bacterium liquid that conventional method is cultivated of initial stage described in the step b is 〉=1.0 * 10
8CFU/ml, pH-value pH are 4.6～5.8; The bacterium number is 〉=1.2 * 10 in the bacillus coagulans Leehai 887 bacterium liquid of fermentation back
7CFU/ml, pH-value pH are 4.3～5.8; The granularity of described analysis for soybean powder is all to sieve by 40～60 mesh sieve analyses.
According to the preparation method of above-mentioned bioactive substance carrier, at least a in peeling soyabean expeller, analysis for soybean powder, soya bean slag, degrease rice bran, the broad bean dregs of rice, the pea dregs of rice, the cottonseed dregs of rice, rapeseed dregs, wheat bran, barley bran, brewex's grains, maize alcohol stillage, rice vinasse and the corn flour of solid-state substrate described in the step c; Described material is pulverized the back and is crossed 20～40 mesh sieves.
A kind of chicken bio-additive premixed feed special, described bio-additive premixed feed is that the bioactivator with above-mentioned preparation is a carrier, and the required raw material of preparation 1000g chicken bio-additive premixed feed special is: bioactive substance carrier 242.0g～397.0g, calcium dihydrogen phosphate 160.0g～267.5g, calcium carbonate 200.0g～280.0g, vitamin(e) supplement 12.5g～30.5g, Amino acid additive 60.0g～72.5g, mineral element 12.5g～25.0g, enzyme preparation 12.5g～20.0g, linolenic acid 80.0g～125.0g, the dry amount is that 20～45% bacillus subtilis Tsao 060607 liquid bioactivator 1.0ml～12.0ml and dry amount are bacillus coagulans Leehai 887 liquid bioactivator 3.0ml～7.8ml of 20～45%.
According to above-mentioned chicken bio-additive premixed feed special, described preparation 1000g chicken bio-additive premixed feed special also need add mold toxin sorbent 12.5g.
According to above-mentioned chicken bio-additive premixed feed special, the content of bacillus subtilis Tsao 060605 is 1.0 * 10 in the described bioactive substance carrier
5～3.0 * 10
10CFU/kg, the content of bacillus coagulans Leehai887 are 1.0 * 10
6～5.0 * 10
11CFU/kg.
According to above-mentioned chicken bio-additive premixed feed special, the content of bacillus subtilis Tsao 060605 is 1.0 * 10 in the described chicken bio-additive premixed feed special
4～2.0 * 10
10CFU/kg, the content of bacillus coagulans Leehai 887 are 1.0 * 10
5～2.0 * 10
11CFU/kg.
According to above-mentioned chicken bio-additive premixed feed special, described dry amount is that 20～45% bacillus subtilis Tsao 06065 liquid bioactivator is that the bacillus subtilis Tsao 060605 liquid bioactivator that will prepare among the claim 1 step a concentrates according to conventional method and forms; Described dry amount is that 20～45% bacillus coagulans Leehai 887 liquid bioactivators are that the bacillus coagulans Leehai 887 liquid bioactivators that will prepare among the claim 1 step b concentrate according to conventional method and form.
According to above-mentioned chicken bio-additive premixed feed special, described vitamin(e) supplement, Amino acid additive, mineral element, enzyme preparation and mold toxin sorbent are the national regulation standardized product of selling on the market.
The nutritional labeling that contains in the seawood meal that the present invention adopts sees Table one.
Table one: seawood meal nutritional labeling
The present invention adds seawood meal in solid-state bioactivator preparation process, more trace element and Amino acid can be provided.
Positive beneficial effect of the present invention:
1, the chicken bio-additive premixed feed special of the present invention's preparation is with natural grain that the useful bacterial classification of animal is fermented, so premixed feed of the present invention is the green feed of pure natural.The disposable metabolite that probiotics produces in fermentation in the preparation process is nontoxic, wherein has many products can reduce antibiotic working strength and frequency in the chicken feed; Premixed feed of the present invention can make an addition to the chicken feed with 1～4% ratio normality, but can significantly promote digestibility, the feed available energy of protein and Amino acid in the feed thus, and reduce diarrhea to take place and impel ight soil to do and pine, reduce ammonia and hydrogen sulfide stink, promote chicken grow, improve food conversion ratio, increase egg size and eggshell hardness, prolongation laying hen egg-laying peak, enhancing immunological stress ability.
2, the bacillus subtilis Tsao 060605 that adopts of the present invention has the following advantages: (1), can produce endogenous spore, high temperature resistant, hydrochloric acid in gastric juice, cholate; (2), because propagation generation quick, can effectively suppress growth of pathogenic bacteria; (3), secretion antibacterial material ability is strong, can suppress pathogens such as Escherichia coli, salmonella, staphylococcus aureus; (4), the secretase ability is strong, comprises AMS (Ye Huamei), gamma amylase (carbohydrase), cellulase and protease; (5), reduce spoilage organisms excessive fermentation in large intestine and produce the animal harm that toxin caused; (6), can utilize the carbohydrate in the feed to change into organic acid, effectively promote enterocinesia, the prevention constipation.
3, the bacillus coagulans Leehai 887 that adopts of the present invention has the following advantages: (1), collaborative to make the dominant counterbalance effect of intestinal beneficial bacterium quite strong with bacillus subtilis Tsao 060605, the few candy that promptly makes full use of bacillus subtilis Tsao 060605 decomposition carbohydrate and produce, produce acetate, lactic acid except the sour environment of making the unfavorable growth of pathogen, also provide other lactic acid bacteria good growth factor; (2), strengthen immunity; (3), can make sour environment, the bioavailability of copper, iron, zinc, magnesium, calcium, phosphorus is improved at enteron aisle.
4, the prescription of additive premix of the present invention has singularity, the preparation technology of its bioactive substance carrier has advance, novelty, and effectively reduce the cost of complete feed, must drive chicken aquaculture march toward green, environmental protection, organic, have far-reaching starting and be worth and inspire meaning.
Four, the specific embodiment:
Following examples only are further explained explanation to the present invention, do not limit content of the present invention.
The detailed process with the conventional cultural method of initial stage of relevant performance and two kinds of bacterium liquid obtained of once bacillus subtilis Tsao 060605, bacillus coagulans Leehai 887 these two kinds of bacterial classifications at first is described.
One, obtaining of bacillus subtilis Tsao 060605, bacillus coagulans Leehai 887 these two kinds of bacterial classifications:
(1), the acquisition of bacillus subtilis (Bacillus subtilis) Tsao 060605
1, the acquisition methods of bacillus subtilis Tsao 060605:
(1) a gram discarded object is got by one fermented bean dregs manufacturing works from the Tainan in Taiwan Province, adds 10mlTrypticase
TMSoy Broth (pancreatin soy broth, Difco, Sparks, U.S.A.: every liter contains tryptone 20.0g, dextrose 1.0g, sodium hydrogen phosphate 2.0g, potassium nitrate 1.0g, pH is transferred to 7.0, and solid medium adds 1.5% agar) in after concussion mixes, with the sterilization of conventional Pasteur's moral sterilization;
(2), the TSB nutrient solution is carried out 10 times of gradient dilutions to 10
-4, each gradient dilution liquid was cultivated 24 hours in 37 ℃ of temperature, carried out the bacterium that tentatively increases of gemma bacterial strain and screened;
(3), get 100 μ l to Trypticase from each dilution tube again
TM(Sparks U.S.A) on the flat board, and smears evenly with L type glass rod Soy Broth for pancreatin soy broth, Difco, cultivates 24 hours for same 37 ℃, again with the single bacterium colony to of a picked at random mode picking new Trypticase
TMOn the Soy Agar flat board, and scratch, repeat to obtain the bacterial strain that a strain has the gemma characteristic, with its called after Tsao 060605 after the purifying subculture three times with three-step approach.
2, the characteristic of bacillus subtilis Tsao 060605 bacterial classification:
(1), morphological feature: the bacterium colony rough surface is opaque, fold is arranged and the surface dimness, and for faint yellow or filbert, the outward appearance kenel is rounded or irregular; When growing in the liquid medium within, the normal wrinkle mould that forms.The cell size is (0.6～0.8 μ m) * (1.5～3 μ m) approximately, Gram-positive, uniform coloring, no pod membrane; Interior type spore size (0.3～0.9 μ m) * (1.1～1.6 μ m) approximately that give birth to, the spore ovalize is positioned at thalline central authorities or inclined to one side slightly, and thalline did not expand after gemma formed; Usually utilize peritrichous to move.
(2), physiological property: chemical different battalion, aerobic metabolism; Can carry out effect of separating of good sugar and TCA circulation, (catalase) is positive for catalase; The ability of tool aminosal, fiber, half fiber and starch, the carbohydrate of degrading produces abundant acid; At 28～60 ℃, pH-value 6.5～7.0 o'clock well-grown; Tool is withstand voltage, heat-resisting, acidproof, alkaline-resisting characteristic; In the environment that lacks carbon, nitrogen or phosphorus, can form endogenous spore.
(3), heat endurance: the heat endurance of bacillus subtilis (Bacillus subtilis) Tsao 060605 sees Table 1.
Table 1: the heat endurance of bacillus subtilis Tsao 060605
Annotate: the initial gemma bacterium number of specimen is 2.5 * 10
8CFU/ml.
(5), resistance to acids and bases:
10ml bacillus subtilis (Bacillus subtilis) Tsao 060605 bacterium liquid is through centrifugal removal supernatant, and the bacterium number that records is 2.5 * 10
8CFU/ml, (pH2～7 buffer solutions are to use the sodium hydrogen phosphate salting liquid of 0.2M and the hydrochloric acid solution of 0.2M to adjust in the buffer solution of different pH values respectively, pH8～10 buffer solutions are to use the sodium hydrogen phosphate salting liquid of 0.2M and the sodium hydroxide solution of 0.2M to adjust), left standstill 3 hours, add up the bacterium number then, the results are shown in Table 2.
The resistance to acids and bases of table 2, bacillus subtilis Tsao 060605
| PH-value pH | Remaining bacterium number (CFU/ml) | PH-value pH | Remaining bacterium number (CFU/ml) |
| ??2 | ??1.5×10 8CFU/ml | ??7 | ??2.5×10 8CFU/ml |
| ??3 | ??1.8×10 8CFU/ml | ??8 | ??2.5×10 8CFU/ml |
| ??4 | ??2.5×10 8CFU/ml | ??9 | ??2.5×10 8CFU/ml |
| ??5 | ??2.5×10 8CFU/ml | ??10 | ??2.5×10 8CFU/ml |
| ??6 | ??2.5×10 8CFU/ml |
Annotate: the starter bacteria number of specimen is 2.5 * 10
8CFU/ml.
(6), optimum medium composition:
Trypticase
TMConsisting of of Soy Broth pancreatin soy broth: tryptone 17.0g, phytone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, adding distil water are mixed with 1 liter, adjust pH7.0.
(7), the suitableeest cultivation temperature: the suitableeest cultivation temperature is 37 ℃.
(2), obtaining of bacillus coagulans Leehai 887:
1, the acquisition methods of bacillus coagulans Leehai 887:
(1), a gram sample is got by one fertilizer manufactory from the Kao-hsiung county, adds 10ml GYE Broth (GYE nutrient solution; Contain yeast extract 5.0g, glucose 2.0g, monopotassium phosphate 0.5g, dikalium phosphate 0.5g, magnesium sulfate 0.3g, sodium chloride 0.01g, manganese sulfate 0.01g, zinc sulfate 0.0016g, copper sulphate 0.0016g, cobaltous sulfate 0.0016g, meat peptone 5g, pH is transferred to 6.0～6.2, solid medium adds 1.5% agar) in after concussion mixes, at once cool to 45 ℃ with 70 ℃ of waters bath with thermostatic control after 30 minutes.
(2), carry out 10 times of gradient dilutions to 10 with the GYE nutrient solution again
-6, each gradient dilution liquid is incubated at 40 ℃ of temperature cultivated 48 hours, carry out the bacterium that tentatively increases of gemma bacterial strain and screen.
(3), get 100 μ l to Difco from each dilution tube
TMOn the Lactobacilli MRS plating medium, and smear evenly with L type glass rod, same 37 ℃ of cultivations respectively are after 24 hours, again with the about 2.5mm size of random fashion picking, be on the new MRS plating medium of the single bacterium colony to of projection, surface smoothing light, and scratch with three-step approach, after the repetition purifying subculture three times, obtain the bacterial strain that a strain has the lactic acid producing activity, with its called after Leehai 887.
2, the characteristic of bacillus coagulans Leehai 887 bacterial classifications:
(1), morphological feature: the about 2.0～2.5mm of bacterium colony size, be projection, surface smoothing light, chromogenesis, milky be not to milk yellow.Bacterial classification size (3.0～8.0 μ m) * (1.0～1.9 μ m), very thin elongated rod shape is crooked slightly occasionally, the tool peritrichous, there is motility circle, Gram-positive, the endogenous oval gemma of product position at cell end, spore size pact (1.0～1.7 μ m) * (0.9～1.2 μ m) to end slightly.
(2), physiological property: chemical different battalion, aerobic to little good oxygen metabolism, the sour environment well-grown, row homogeneity type acid ferment also produces L (+) type lactic acid, can produce antibacterial material.Can utilize the minority carbohydrate and produce acid, comprise glucose (Glucose), galactolipin (Galactose), wood sugar (Xylose), arabinose (Arabinose), maltose (Maltose), mannose (Mannose), fructose (Fructose), sucrose (Sucrose) and trehalose (Trehalose).Can't hydrolyzed starch and casein.Catalase (Catalase) reaction is positive.Drawing piece (Indole) reacts negative, does not produce hydrogen sulfide.Can't degrade nitrate become nitrite.At 30～37 ℃, pH-value 5.5～6.5 o'clock is the well-grown condition.
(3), heat endurance:
The heat endurance of bacillus coagulans (Bacillus coagulans) Leehai 887 sees Table 3.
The heat endurance of table 3, bacillus coagulans Leehai 887
Annotate: the initial gemma bacterium number of specimen is 2.6 * 10
8CFU/ml.
(4), resistance to acids and bases:
(pH2～7 buffer solutions are to use the sodium hydrogen phosphate salting liquid of 0.2M and the hydrochloric acid solution of 0.2M to adjust in the buffer solution of different pH values with bacillus coagulans (Bacillus coagulans) Leehai 887 respectively, pH8～10 buffer solutions are to use the sodium hydrogen phosphate salting liquid of 0.2M and the sodium hydroxide solution of 0.2M to adjust), left standstill 3 hours, add up the bacterium number then, the results are shown in Table 4.
The resistance to acids and bases of table 4, bacillus coagulans Leehai 887
| PH-value pH | Remaining bacterium number (CFU/ml) | PH-value pH | Remaining bacterium number (CFU/ml) |
| ??2 | ??3.8×10 7CFU/ml | ??7 | ??2.9×10 8CFU/ml |
| ??3 | ??1.7×10 8CFU/ml | ??8 | ??2.9×10 8CFU/ml |
| ??4 | ??2.6×10 8CFU/ml | ??9 | ??2.8×10 8CFU/ml |
| ??5 | ??2.9×10 8CFU/ml | ??10 | ??2.7×10 8CFU/ml |
| ??6 | ??2.9×10 8CFU/ml |
Annotate: the starter bacteria number of specimen is 2.9 * 10
8CFU/ml.
(5), optimum medium composition:
Consisting of of Lactobacilli MRS culture medium: contain peptone 10.0g in every liter of culture medium, beef extract extract 10.0g, yeast extract 5.0g, glucose 20.0g, ammonium citrate 2.0g, sodium acetate 5.0g, 7 times of Magnesium sulfate heptahydrate 0.1g, hydrated manganese sulfate 0.05g, anhydrous phosphoric acid dipotassium 2.0g, Tween-80 1.0g; The distilled water preparation, pH6.5.
(6), the suitableeest cultivation temperature: the suitableeest cultivation temperature is 37 ℃.
Two, the detailed process of conventional cultural method of the initial stage of bacillus subtilis Tsao 060605 bacterium liquid:
The first step: cultivate bacillus subtilis Tsao 060605 as kind of a bottle with the bottled 100ml pancreatin of 250ml pyrometric cone soy broth, carry out the preparation of kind of bacterium, in 37 ℃ of isothermal vibration incubators,, make the bacterium number reach 1.0 * 10 with 150rpm concussion speed, 24 hours time
8More than the CFU/ml.
Second step: will plant bottle bacterium liquid and pour the stirring-type fermentation tank that 5 liters of pancreatin soy broth are housed into, mixing speed is controlled at 150rpm, throughput 0.5v/v.min, and temperature remains on 37 ℃ of constant temperature, 24 hours time, makes the bacterium number reach 1.0 * 10
8CFU/ml～3.0 * 10
9CFU/ml, pH-value pH5.8～6.5.
The 3rd step: in seeding tank, ferment
The seeding tank that will be inoculated into 500L through the bacterium liquid of the bacillus subtilis Tsao 060605 after 5 liters of stirring-type fermentation tank activation ferments.Fermentation condition is as follows:
The 1-1 culture medium:
1-1-1, concentration are measured as the molasses of 7.0brix, skimmed milk power 0.8% with saccharometer;
1-1-2 or concentration are measured as the molasses of 2.5brix, analysis for soybean powder 2.1% with saccharometer;
1-1-3 or concentration are measured as the soya-bean milk of 4.5brix with saccharometer.
With the practical operation is example, adopts the molasses of 2.5brix, and analysis for soybean powder 2.1% is a seed fermentation jar culture medium.
1-2,37 ℃ of ferment at constant temperature 24 hours, mixing speed is controlled at 150rpm, and throughput 0.5v/v.min regulates pH-value in the sweat with 0.5M NaOH.
The bacterium number of 1-3, fermentation termination reaches 1.2 * 10
7More than the CFU/ml, pH-value pH5.8～6.5.
Three, the detailed process of conventional cultural method of the initial stage of bacillus coagulans Leehai 887 bacterium liquid:
The first step: cultivate bacillus coagulans Leehai 887 as kind of a bottle with the bottled 175ml MRS of 250ml pyrometric cone nutrient solution, carry out the preparation of kind of bacterium, in 37 ℃ of isothermal vibration incubators,, make the bacterium number reach 1.0 * 10 with 100rpm concussion speed, 24 hours time
8More than the CFU/ml.
Second step: will plant bottle bacterium liquid and pour the stirring-type fermentation tank that 5 liters of MRS nutrient solutions are housed into, mixing speed is controlled at 100rpm, throughput 0.1v/v.min, temperature remains on 37 ℃ of constant temperature, 24 hours time,, make the bacterium number reach 1.0 * 10 with 0.1M NaOH feedback material control pH-value
8CFU/ml～4.5 * 10
8CFU/ml, pH-value pH4.3～5.8.
The 3rd step: in seeding tank, ferment
The seeding tank that will be inoculated into 500L through the bacterium liquid of the bacillus coagulans Leehai 887 after 5 liters of stirring-type fermentation tank activation ferments.Fermentation condition is as follows:
The 1-1 culture medium:
1-1-1, concentration are measured as the molasses of 9.0brix, skimmed milk power 0.8% with saccharometer;
1-1-2 or concentration are measured as the molasses of 2.5brix, analysis for soybean powder 1.5% with saccharometer;
1-1-3 or concentration are measured as the soya-bean milk of 4.5brix with saccharometer.
1-1-4, be example, adopt the molasses of 2.5brix with the practical operation, analysis for soybean powder 1.5%, anhydrous phosphoric acid dipotassium 0.2% is a seed fermentation jar culture medium.
1-2,37 ℃ of ferment at constant temperature 24 hours, mixing speed is controlled at 100rpm, and throughput 0.1v/v.min regulates pH-value in the sweat with 0.5M NaOH.
The bacterium number of 1-3, fermentation termination reaches 1.2 * 10
7More than the CFU/ml, pH-value pH4.3～5.8.
Embodiment 1:
The preparation method of bioactive substance carrier of the present invention, this preparation method's detailed step is as follows:
The preparation of a, bacillus subtilis Tsao 060605 liquid bioactivator:
Being diluted to saccharometer measurement concentration with sterilized water molasses earlier is 2.5brix, under 100 ℃ of conditions, sterilization treatment is after 15 minutes, get 3700L and put into fermentation tank, (bacillus subtilis Tsao 060605 bacterium number is 〉=1.0 * 10 to the bacillus subtilis Tsao 060605 bacterium liquid that the initial stage is cultivated through conventional method in this bacterium liquid again
8CFU/ml, pH-value pH is 5.8～6.5) 500L pours fermentation tank into, and then in sterilization tank, being that 7% the skimmed milk power nutrient source aqueous solution is with 100 ℃ with the concentration of 1000L sterilized water preparation, after 15 minutes sterilization treatment, get 800L and put into fermentation tank, making skimmed milk power after the adding is 1.2% with respect to its concentration of molasses, comprehensive above nutrient source prepares liquid substrate and ferments, fermentation temperature is 37 ℃ when fermentation cylinder for fermentation, fermentation time is that (mixing speed was controlled at 150rpm in the sweat in 24 hours, throughput is 0.5v/v.min, with the pH-value in the NaOH adjusting sweat of 0.5mol/L), when stopping, fermentation adds catabolic enzyme (catabolic enzyme adopt be cellulolytic enzyme), its addition is to account for 0.01% of solid-state substrate total amount described in the step c, when adding catabolic enzyme, with ice water circulation the zymocyte liquid temperature is reduced to 7～8 ℃ in the fermentation tank chuck and get final product (fermentation afterwards in the bacillus subtilis Tsao 060605 bacterium liquid bacterium number be 〉=1.2 * 10
7CFU/ml, pH-value pH are 5.8～6.5);
The preparation of b, bacillus coagulans Leehai 887 liquid bioactivators:
Being diluted to saccharometer measurement concentration with sterilized water molasses earlier is 2.0brix, under 100 ℃ of conditions, sterilization treatment is after 15 minutes, get 3500L and put into fermentation tank, (bacillus coagulans Leehai 887 bacterium numbers are 〉=1.0 * 10 to the bacillus coagulans Leehai 887 bacterium liquid that the initial stage is cultivated through conventional method in this bacterium liquid again
8CFU/ml, pH-value pH is 4.6～5.8) 500L pours fermentation tank into, again the skimmed milk power that with the concentration of 1000L sterilized water preparation is 8% in sterilization tank, 1% the anhydrous phosphoric acid dipotassium nutrient source aqueous solution, with 100 ℃, get 1000L after 15 minutes sterilization treatment and put into fermentation tank, make skimmed milk power after the adding, the anhydrous phosphoric acid dipotassium reaches 1.8% and 0.2% respectively with respect to its concentration of molasses, comprehensive above nutrient source prepares liquid substrate and ferments, fermentation temperature is 37 ℃ during fermentation, fermentation time is that (mixing speed was controlled at 100rpm in the sweat in 42 hours, throughput is 0.1v/v.min, with the pH-value in the NaOH adjusting sweat of 0.5mol/L), when stopping, fermentation adds bromelain and alpha-galactosidase, the addition of bromelain accounts for 0.0005% of solid-state substrate total amount described in the step c, the addition of described alpha-galactosidase accounts for 0.05% of solid-state substrate total amount described in the step c, when adding enzyme, with ice water circulation the zymocyte liquid temperature is reduced to 7～8 ℃ in the fermentation tank chuck and get final product (fermentation afterwards in the bacillus coagulans Leehai 887 bacterium liquid bacterium number be 〉=1.2 * 10
7CFU/ml, pH-value pH are 4.3～5.8);
The preparation of c, bacillus subtilis Tsao 060605 and bacillus coagulans Leehai 887 solid-state bioactivators:
Earlier with solid-state substrate (described solid-state substrate is peeling soyabean expeller and soyabean expeller) modified 10～15 minutes with steam, be cooled to 40～43 ℃, then the bacillus subtilis Tsao 060605 liquid bioactivator of step a preparation and the bacillus coagulans Leehai 887 liquid bioactivators of step b preparation are mixed, the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator is 30% during mixing, solid-state substrate and mixed liquid bioactivator after handling are carried out mixed culture fermentation, when solid-state substrate mixes with mixed liquid bioactivator, the shared weight percentage of wherein mixed liquid bioactivator is 40%, fermentation temperature is 37 ℃ during fermentation, fermentation time is 24 hours, finish preceding 6 hours adding seawood meals at solid state fermentation, the addition of described seawood meal accounts for 7% of solid-state substrate total amount, after the fermentation ends its material is dried under 70 ℃ temperature, dry to fermentation substrate moisture≤7%, pulverize 40 mesh sieves at last, obtained the solid state fermentation bioactivator;
D, the preparation of bioactive substance carrier: with step a, it is 25% liquid state fermentation bioactivator that the bacillus subtilis Tsao 060605 liquid bioactivator of b preparation and bacillus coagulans Leehai 887 liquid bioactivators are condensed into the dry amount respectively, liquid state fermentation bioactivator after two kinds concentrated mixes, wherein the dry amount is that 25% bacillus subtilis Tsao 060605 liquid bioactivator is shared that weight percentage be 45%, mixed liquid state fermentation bioactivator is sprayed on the solid state fermentation bioactivator for preparing among the step c, described mixed liquid state fermentation bioactivator sprinkling amount accounts for 1.0% of solid-state biofermentation active material total amount, promptly gets bioactive substance carrier after the sprinkling.
A kind of chicken bio-additive premixed feed special, this bio-additive premixed feed is that the bioactivator with above-mentioned preparation is a carrier, and the required raw material of preparation 1000g premixed feed is: bioactive substance carrier 250.0g, calcium dihydrogen phosphate 267.5g, calcium carbonate 260.0g, vitamin(e) supplement 25.0g, Amino acid additive 60.0g, mineral element 12.5g, enzyme preparation 12.5g, mold toxin sorbent 12.5g, linolenic acid 80.0g, the dry amount is that 25% bacillus subtilis Tsao 060607 liquid bioactivator 4.5ml and dry amount are 25% bacillus coagulans Leehai 887 liquid bioactivator 5.5ml.
The vitamin(e) supplement that adopts in the chicken bio-additive premixed feed special, Amino acid additive, mineral element, enzyme preparation and mold toxin sorbent are the national regulation standardized product of selling on the market.
Embodiment 2:
The preparation method of bioactive substance carrier of the present invention and embodiment one are basic identical, and difference is:
Step a: it is 3brix that molasses are diluted to concentration with sterilized water, get 3500L after the molasses sterilization treatment of dilution and put into fermentation tank, get the skimmed milk power nutrient source aqueous solution after the 1000L sterilization treatment, skimmed milk power is 1.5% with respect to the concentration of molasses after adding, fermentation temperature is 27 ℃, fermentation time is 48 hours, and the catabolic enzyme of adding is the alphalise starch catabolic enzyme, and its addition accounts for 0.05% of solid-state substrate total amount that step c adopted;
Step b: earlier molasses being diluted to concentration with sterilized water is 3brix, get 4200L after the molasses sterilization treatment of dilution and put into fermentation tank, get skimmed milk power, the anhydrous phosphoric acid dipotassium nutrient source aqueous solution after the 300L sterilization treatment, skimmed milk power, anhydrous phosphoric acid dipotassium are respectively 0.7%, 0.08% with respect to the concentration of molasses after adding, fermentation temperature is 42 ℃, fermentation time is 18 hours, the addition of bromelain accounts for 0.001% of the solid-state substrate total amount that adopts among the step c, and the addition of alpha-galactosidase accounts for 0.05% of solid-state substrate total amount described in the step c;
Among the step c: solid-state substrate is an analysis for soybean powder, the mixture of the soya bean slag and the broad bean dregs of rice, with its solid-state substrate modified 5～10 minutes with steam, the shared weight percentage of the liquid bioactivator of bacillus subtilis Tsao060605 was 10% when two kinds of liquid bioactivators mixed, when solid-state substrate mixes with mixed liquid bioactivator, the shared weight percentage of mixed liquid bioactivator is 70%, fermentation temperature is 50 ℃, fermentation time is 18 hours, finish preceding 4 hours adding seawood meals at solid state fermentation, the addition of seawood meal accounts for 6.5% of solid-state substrate total amount, and material is dried under 100 ℃ of conditions after the fermentation ends;
Steps d: it is 30% liquid state fermentation bioactivator that two kinds of liquid bioactivators of step a, b preparation are condensed into the dry amount respectively, when two kinds of liquid state fermentation bioactivators after concentrating mix, the dry amount is that 30% the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator is 50%, and the sprinkling amount of mixed liquid state fermentation bioactivator accounts for 0.5% of solid state fermentation bioactivator total amount.
A kind of chicken bio-additive premixed feed special, this bio-additive premixed feed is that the bioactivator with above-mentioned preparation is a carrier, and the required raw material of preparation 1000g premixed feed is: bioactive substance carrier 315.0g, calcium dihydrogen phosphate 200.0g, calcium carbonate 245.0g, vitamin(e) supplement 25.0g, Amino acid additive 60.0g, mineral element 12.5g, enzyme preparation 20.0g, mold toxin sorbent 12.5g, linolenic acid 90.0g, the dry amount is that 30% bacillus subtilis Tsao 060607 liquid bioactivator 5.0ml and dry amount are 30% bacillus coagulans Leehai 887 liquid bioactivator 5.0ml.
Embodiment 3:
The preparation method of bioactive substance carrier of the present invention and embodiment one are basic identical, and difference is:
Step a: it is 7.0brix that molasses are diluted to concentration with sterilized water, get 3800L after the molasses sterilization treatment of dilution and put into fermentation tank, get the skimmed milk power nutrient source aqueous solution after the 700L sterilization treatment, skimmed milk power is 1.1% with respect to the concentration of molasses after adding, fermentation temperature is 55 ℃, fermentation time is 10 hours, and the catabolic enzyme of adding is half cellulolytic enzyme, and its addition accounts for 0.10% of solid-state substrate total amount that step c adopted;
Step b: earlier molasses being diluted to concentration with sterilized water is 9.0brix, get 3800L after the molasses sterilization treatment of dilution and put into fermentation tank, get skimmed milk power, the anhydrous phosphoric acid dipotassium nutrient source aqueous solution after the 700L sterilization treatment, skimmed milk power, anhydrous phosphoric acid dipotassium are respectively 1.2%, 0.15% with respect to the concentration of molasses after adding, fermentation temperature is 40 ℃, fermentation time is 35 hours, the addition of bromelain accounts for 0.005% of the solid-state substrate total amount that adopts among the step c, and the addition of described alpha-galactosidase accounts for 0.01% of solid-state substrate total amount described in the step c;
Among the step c: solid-state substrate is an analysis for soybean powder, with its solid-state substrate modified 15～20 minutes with steam, the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator was 20% when two kinds of liquid bioactivators mixed, when solid-state substrate mixes with mixed liquid bioactivator, the shared weight percentage of mixed liquid bioactivator is 50%, fermentation temperature is 30 ℃, fermentation time is 72 hours, finish preceding 8 hours adding seawood meals at solid state fermentation, the addition of seawood meal accounts for 10% of solid-state substrate total amount, and material is dried under 70 ℃ of conditions after the fermentation ends;
Steps d: it is 40% liquid state fermentation bioactivator that two kinds of liquid bioactivators of step a, b preparation are condensed into the dry amount respectively, when two kinds of liquid state fermentation bioactivators after concentrating mix, the dry amount is that 40% the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator is 40%, and the sprinkling amount of mixed liquid state fermentation bioactivator accounts for 1.0% of solid state fermentation bioactivator total amount.
A kind of chicken bio-additive premixed feed special, this bio-additive premixed feed is that the bioactivator with above-mentioned preparation is a carrier, and the required raw material of preparation 1000g premixed feed is: bioactive substance carrier 242.0g, calcium dihydrogen phosphate 195.0g, calcium carbonate 280.0g, vitamin(e) supplement 30.5g, Amino acid additive 70.0g, mineral element 12.5g, enzyme preparation 12.5g, mold toxin sorbent 12.5g, linolenic acid 125.0g, the dry amount is that 40% bacillus subtilis Tsao 060607 liquid bioactivator 4.0ml and dry amount are 40% bacillus coagulans Leehai 887 liquid bioactivator 6.0ml.
Embodiment 4:
The preparation method of bioactive substance carrier of the present invention and embodiment one are basic identical, and difference is:
Step a: it is 1.0brix that molasses are diluted to concentration with sterilized water, get 3600L after the molasses sterilization treatment of dilution and put into fermentation tank, the analysis for soybean powder of getting 5% after the 900L sterilization treatment (includes concentration and is 0.001% pineapple ferment, the granularity of analysis for soybean powder is all by 40～60 mesh sieve analyses sieve) the nutrient source aqueous solution, analysis for soybean powder is 1% with respect to the concentration of molasses after adding, fermentation temperature is 30 ℃, fermentation time is 30 hours, the catabolic enzyme that adds is the Xylans enzyme, and addition accounts for 0.20% of solid-state substrate total amount that step c adopted;
Step b: earlier molasses being diluted to concentration with sterilized water is 1.5brix, get 4000L after the molasses sterilization treatment of dilution and put into fermentation tank, the analysis for soybean powder of getting 7% after the 500L sterilization treatment (includes concentration and is 0.001% pineapple ferment, the granularity of analysis for soybean powder is all by 40～60 mesh sieve analyses sieve), the anhydrous phosphoric acid dipotassium nutrient source aqueous solution, add the back analysis for soybean powder, the anhydrous phosphoric acid dipotassium is respectively 0.8% with respect to the concentration of molasses, 0.1%, fermentation temperature is 40 ℃, fermentation time is 20 hours, the addition of bromelain accounts for 0.01% of the solid-state substrate total amount that adopts among the step c, and the addition of described alpha-galactosidase accounts for 0.01% of solid-state substrate total amount described in the step c;
Among the step c: solid-state substrate is the mixture of the broad bean dregs of rice and the pea dregs of rice, with its solid-state substrate modified 20～25 minutes with steam, the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator was 30% when two kinds of liquid bioactivators mixed, when solid-state substrate mixes with mixed liquid bioactivator, the shared weight percentage of mixed liquid bioactivator is 50%, fermentation temperature is 55 ℃, fermentation time is 12 hours, finish preceding 4 hours adding seawood meals at solid state fermentation, the addition of seawood meal accounts for 4% of solid-state substrate total amount, and material is dried under 55 ℃ of conditions after the fermentation ends;
Steps d: it is 40% liquid state fermentation bioactivator that two kinds of liquid bioactivators of step a, b preparation are condensed into the dry amount respectively, when two kinds of liquid state fermentation bioactivators after concentrating mix, the dry amount is that 40% the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator is 50%, and the sprinkling amount of mixed liquid state fermentation bioactivator accounts for 1.5% of solid state fermentation bioactivator total amount.
A kind of chicken bio-additive premixed feed special, this bio-additive premixed feed is that the bioactivator with above-mentioned preparation is a carrier, and the required raw material of preparation 1000g premixed feed is: bioactive substance carrier 320.0g, calcium dihydrogen phosphate 215.0g, calcium carbonate 230.0g, vitamin(e) supplement 25.0g, Amino acid additive 60.0g, mineral element 12.5g, enzyme preparation 15.0g, mold toxin sorbent 12.5g, linolenic acid 90.0g, the dry amount is that 40% bacillus subtilis Tsao 060607 liquid bioactivator 12.0ml and dry amount are 40% bacillus coagulans Leehai 887 liquid bioactivator 3.0ml.
Embodiment 5:
The preparation method of bioactive substance carrier of the present invention and embodiment one are basic identical, and difference is:
Step a: it is 5brix that soya-bean milk is diluted to concentration with sterilized water, get 3900L after the soya-bean milk sterilization treatment of dilution and put into fermentation tank, the analysis for soybean powder of getting 5% after the 600L sterilization treatment (includes concentration and is 0.001% pineapple ferment, the granularity of analysis for soybean powder is all by 40～60 mesh sieve analyses sieve) the nutrient source aqueous solution, analysis for soybean powder is 0.7% with respect to the concentration of soya-bean milk after adding, fermentation temperature is 40 ℃, fermentation time is 28 hours, the catabolic enzyme that adds is the poly-candy enzyme of sweet dew, and its addition accounts for 0.30% of solid-state substrate total amount that step c adopted;
Step b: earlier soya-bean milk being diluted to concentration with sterilized water is 4.5brix, get 3600L after the soya-bean milk sterilization treatment of dilution and put into fermentation tank, get skimmed milk power, the anhydrous phosphoric acid dipotassium nutrient source aqueous solution after the 900L sterilization treatment, skimmed milk power, anhydrous phosphoric acid dipotassium are respectively 1.6%, 0.2% with respect to the concentration of soya-bean milk after adding, fermentation temperature is 37 ℃, fermentation time is 24 hours, the addition of bromelain accounts for 0.001% of the solid-state substrate total amount that adopts among the step c, and the addition of described alpha-galactosidase accounts for 0.04% of solid-state substrate total amount described in the step c;
Among the step c: solid-state substrate is the mixture of the broad bean dregs of rice and the pea dregs of rice, with its solid-state substrate modified 25～30 minutes with steam, the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator was 25% when two kinds of liquid bioactivators mixed, when solid-state substrate mixes with mixed liquid bioactivator, the shared weight percentage of mixed liquid bioactivator is 25%, fermentation temperature is 42 ℃, fermentation time is 36 hours, finish preceding 2 hours adding seawood meals at solid state fermentation, the addition of seawood meal accounts for 10% of solid-state substrate total amount, and material is dried under 90 ℃ of conditions after the fermentation ends;
Steps d: it is 45% liquid state fermentation bioactivator that two kinds of liquid bioactivators of step a, b preparation are condensed into the dry amount respectively, when two kinds of liquid state fermentation bioactivators after concentrating mix, the dry amount is that 45% the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator is 48%, and the sprinkling amount of mixed liquid state fermentation bioactivator accounts for 1.5% of solid state fermentation bioactivator total amount.
A kind of chicken bio-additive premixed feed special, this bio-additive premixed feed is that the bioactivator with above-mentioned preparation is a carrier, and the required raw material of preparation 1000g premixed feed is: bioactive substance carrier 325.0g, calcium dihydrogen phosphate 160.0g, calcium carbonate 260.0g, vitamin(e) supplement 12.5g, Amino acid additive 72.5g, mineral element 25.0g, enzyme preparation 12.5g, mold toxin sorbent 12.5g, linolenic acid 100.0g, the dry amount is that 45% bacillus subtilis Tsao 060607 liquid bioactivator 10.8ml and dry amount are 45% bacillus coagulans Leehai 887 liquid bioactivator 7.2ml.
Embodiment 6:
The preparation method of bioactive substance carrier of the present invention and embodiment one are basic identical, and difference is:
Step a: it is 4.5brix that soya-bean milk is diluted to concentration with sterilized water, get 4000L after the soya-bean milk sterilization treatment of dilution and put into fermentation tank, get after the 500L sterilization treatment 5% analysis for soybean powder and (include concentration and be 0.001% pineapple ferment, the granularity of analysis for soybean powder is all by 40～60 mesh sieve analyses sieve) the nutrient source aqueous solution, analysis for soybean powder is 0.6% with respect to the concentration of soya-bean milk after adding, fermentation temperature is 40 ℃, fermentation time is 22 hours, the catabolic enzyme that adds is cellulolytic enzyme and half cellulolytic enzyme, and its addition accounts for 0.40% of solid-state substrate total amount that step c adopted;
Step b: earlier soya-bean milk being diluted to concentration with sterilized water is 4brix, get 3900L after the soya-bean milk sterilization treatment of dilution and put into fermentation tank, get skimmed milk power, the anhydrous phosphoric acid dipotassium nutrient source aqueous solution after the 600L sterilization treatment, skimmed milk power, anhydrous phosphoric acid dipotassium are respectively 1.1%, 0.1% with respect to the concentration of soya-bean milk after adding, fermentation temperature is 42 ℃, fermentation time is 18 hours, the addition of bromelain accounts for 0.005% of the solid-state substrate total amount that adopts among the step c, and the addition of described alpha-galactosidase accounts for 0.005% of solid-state substrate total amount described in the step c;
Among the step c: solid-state substrate is the peeling soyabean expeller, analysis for soybean powder, the mixture of soya bean slag and wheat bran, with its solid-state substrate modified 10～15 minutes with steam, the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator was 15% when two kinds of liquid bioactivators mixed, when solid-state substrate mixes with mixed liquid bioactivator, the shared weight percentage of mixed liquid bioactivator is 65%, fermentation temperature is 40 ℃, fermentation time is 20 hours, finish preceding 6 hours adding seawood meals at solid state fermentation, the addition of seawood meal accounts for 2% of solid-state substrate total amount, and material is dried under 100 ℃ of conditions after the fermentation ends;
Steps d: it is 35% liquid state fermentation bioactivator that two kinds of liquid bioactivators of step a, b preparation are condensed into the dry amount respectively, when two kinds of liquid state fermentation bioactivators after concentrating mix, the dry amount is that 35% the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator is 40%, and the sprinkling amount of mixed liquid state fermentation bioactivator accounts for 0.5% of solid state fermentation bioactivator total amount.
A kind of chicken bio-additive premixed feed special, this bio-additive premixed feed is that the bioactivator with above-mentioned preparation is a carrier, and the required raw material of preparation 1000g premixed feed is: bioactive substance carrier 387.0g, calcium dihydrogen phosphate 190.5g, calcium carbonate 200.0g, vitamin(e) supplement 12.5g, Amino acid additive 60.0g, mineral element 12.5g, enzyme preparation 15.0g, mold toxin sorbent 12.5g, linolenic acid 90.0g, the dry amount is that 35% bacillus subtilis Tsao 060607 liquid bioactivator 1.0ml and dry amount are 35% bacillus coagulans Leehai 887 liquid bioactivator 4.0ml.
Embodiment 7:
The preparation method of bioactive substance carrier of the present invention and embodiment one are basic identical, and difference is:
Step a: it is 5.5brix that soya-bean milk is diluted to concentration with sterilized water, get 3500L after the soya-bean milk sterilization treatment of dilution and put into fermentation tank, get the skimmed milk power nutrient source aqueous solution after the 1000L sterilization treatment, skimmed milk power is 1.6% with respect to the concentration of soya-bean milk after adding, fermentation temperature is 50 ℃, fermentation time is 10 hours, and the catabolic enzyme of adding is the mixture of cellulolytic enzyme and Xylans enzyme, and the addition of mixed enzyme accounts for 0.50% of solid-state substrate total amount that step c adopted;
Step b: earlier soya-bean milk being diluted to concentration with sterilized water is 5brix, get 4000L after the soya-bean milk sterilization treatment of dilution and put into fermentation tank, get analysis for soybean powder after the 500L sterilization treatment and (include concentration and be 0.001% pineapple ferment, the granularity of analysis for soybean powder is all by 40～60 mesh sieve analyses sieve), the anhydrous phosphoric acid dipotassium nutrient source aqueous solution, add the back analysis for soybean powder, the anhydrous phosphoric acid dipotassium is respectively 0.7% with respect to the concentration of soya-bean milk, 0.1%, fermentation temperature is 37 ℃, fermentation time is 24 hours, the addition of bromelain accounts for 0.002% of the solid-state substrate total amount that adopts among the step c, and the addition of described alpha-galactosidase accounts for 0.03% of solid-state substrate total amount described in the step c;
Among the step c: solid-state substrate is the peeling soyabean expeller, analysis for soybean powder, the mixture of soya bean slag and wheat bran, with its solid-state substrate modified 10～15 minutes with steam, the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator was 25% when two kinds of liquid bioactivators mixed, when solid-state substrate mixes with mixed liquid bioactivator, the shared weight percentage of mixed liquid bioactivator is 95%, fermentation temperature is 45 ℃, fermentation time is 16 hours, finish preceding 6 hours adding seawood meals at solid state fermentation, the addition of seawood meal accounts for 1% of solid-state substrate total amount, and material is dried under 90 ℃ of conditions after the fermentation ends;
Steps d: it is 35% liquid state fermentation bioactivator that two kinds of liquid bioactivators of step a, b preparation are condensed into the dry amount respectively, when two kinds of liquid state fermentation bioactivators after concentrating mix, the dry amount is that 35% the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator is 40%, and the sprinkling amount of mixed liquid state fermentation bioactivator accounts for 0.8% of solid state fermentation bioactivator total amount.
A kind of chicken bio-additive premixed feed special, this bio-additive premixed feed is that the bioactivator with above-mentioned preparation is a carrier, and the required raw material of preparation 1000g premixed feed is: bioactive substance carrier 257.0g, calcium dihydrogen phosphate 200.0g, calcium carbonate 260.5g, vitamin(e) supplement 25.0g, Amino acid additive 60.0g, mineral element 25.0g, enzyme preparation 20.0g, mold toxin sorbent 12.5g, linolenic acid 120.0g, the dry amount is that 35% bacillus subtilis Tsao 060607 liquid bioactivator 3.2ml and dry amount are 35% bacillus coagulans Leehai 887 liquid bioactivator 4.8ml.
Embodiment 8:
The preparation method of bioactive substance carrier of the present invention and embodiment one are basic identical, and difference is:
Step a: it is 6brix that soya-bean milk is diluted to concentration with sterilized water, get 3800L after the soya-bean milk sterilization treatment of dilution and put into fermentation tank, get the skimmed milk power nutrient source aqueous solution after the 700L sterilization treatment, skimmed milk power is 1.1% with respect to the concentration of soya-bean milk after adding, fermentation temperature is 37 ℃, fermentation time is 24 hours, and the catabolic enzyme of adding is the mixture of the poly-candy enzyme of cellulolytic enzyme and sweet dew, and the mixed enzyme addition accounts for 0.05% of solid-state substrate total amount that step c adopted;
Step b: earlier soya-bean milk being diluted to concentration with sterilized water is 5.5brix, get 4200L after the soya-bean milk sterilization treatment of dilution and put into fermentation tank, get analysis for soybean powder after the 300L sterilization treatment and (include concentration and be 0.001% pineapple ferment, the granularity of analysis for soybean powder is all by 40～60 mesh sieve analyses sieve), the anhydrous phosphoric acid dipotassium nutrient source aqueous solution, add the back analysis for soybean powder, the anhydrous phosphoric acid dipotassium is respectively 0.6% with respect to the concentration of soya-bean milk, 0.08%, fermentation temperature is 37 ℃, fermentation time is 24 hours, the addition of bromelain accounts for 0.008% of the solid-state substrate total amount that adopts among the step c, and the addition of described alpha-galactosidase accounts for 0.05% of solid-state substrate total amount described in the step c;
Among the step c: solid-state substrate is the peeling soyabean expeller, analysis for soybean powder, the mixture of soya bean slag and rice bran, with its solid-state substrate modified 10～15 minutes with steam, the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator was 30% when two kinds of liquid bioactivators mixed, when solid-state substrate mixes with mixed liquid bioactivator, the shared weight percentage of mixed liquid bioactivator is 30%, fermentation temperature is 37 ℃, fermentation time is 24 hours, finish preceding 6 hours adding seawood meals at solid state fermentation, the addition of seawood meal accounts for 8% of solid-state substrate total amount, and material is dried under 70 ℃ of conditions after the fermentation ends;
Steps d: it is 30% liquid state fermentation bioactivator that two kinds of liquid bioactivators of step a, b preparation are condensed into the dry amount respectively, when two kinds of liquid state fermentation bioactivators after concentrating mix, the dry amount is that 30% the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator is 45%, and the sprinkling amount of mixed liquid state fermentation bioactivator accounts for 1.2% of solid state fermentation bioactivator total amount.
A kind of chicken bio-additive premixed feed special, this bio-additive premixed feed is that the bioactivator with above-mentioned preparation is a carrier, and the required raw material of preparation 1000g premixed feed is: bioactive substance carrier 397.0g, calcium dihydrogen phosphate 160.0g, calcium carbonate 200.0g, vitamin(e) supplement 18.0g, Amino acid additive 65.0g, mineral element 12.5g, enzyme preparation 20.0g, mold toxin sorbent 12.5g, linolenic acid 95.0g, the dry amount is that 30% bacillus subtilis Tsao 060607 liquid bioactivator 4.2ml and dry amount are 30% bacillus coagulans Leehai 887 liquid bioactivator 7.8ml.
Embodiment 9:
The preparation method of bioactive substance carrier of the present invention is identical with embodiment one.
A kind of chicken bio-additive premixed feed special, this bio-additive premixed feed is that the bioactivator with above-mentioned preparation is a carrier, and the required raw material of preparation 1000g premixed feed is: bioactive substance carrier 262.5g, calcium dihydrogen phosphate 267.5g, calcium carbonate 260.0g, vitamin(e) supplement 25.0g, Amino acid additive 60.0g, mineral element 12.5g, enzyme preparation 12.5g, linolenic acid 80.0g, the dry amount is that 25% bacillus subtilis Tsao 060607 liquid bioactivator 4.5ml and dry amount are 25% bacillus coagulans Leehai 887 liquid bioactivator 5.5ml.
Embodiment 10:
The preparation method of bioactive substance carrier of the present invention is identical with embodiment four.
A kind of chicken bio-additive premixed feed special, this bio-additive premixed feed is that the bioactivator with above-mentioned preparation is a carrier, and the required raw material of preparation 1000g premixed feed is: bioactive substance carrier 320.0g, calcium dihydrogen phosphate 215.0g, calcium carbonate 242.5g, vitamin(e) supplement 25.0g, Amino acid additive 60.0g, mineral element 12.5g, enzyme preparation 15.0g, linolenic acid 90.0g, the dry amount is that 40% bacillus subtilis Tsao 060607 liquid bioactivator 12.0ml and dry amount are 40% bacillus coagulans Leehai 887 liquid bioactivator 3.0ml.
Claims (10)
1, a kind of preparation method of bioactive substance carrier is characterized in that, the preparation method of described bioactive substance carrier may further comprise the steps:
A, the preparation of bacillus subtilis Tsao 060605 liquid bioactivator: earlier molasses or soya-bean milk are diluted to sterilized water that to measure concentration with balling hydrometer be 1～7brix, under 100 ℃ of conditions, after the sterilization treatment 12～18 minutes, get 3500～4200L and put into fermentation tank, the bacillus subtilis Tsao 060605 bacterium liquid 500L that initial stage is cultivated through conventional method pours fermentation tank into again, and then in sterilization tank, being that the analysis for soybean powder nutrient source aqueous solution of 7% skimmed milk power or 5% is with 100 ℃ with the concentration of 1000L sterilized water preparation, after 12～18 minutes sterilization treatment, get 300～1000L and put into fermentation tank, make described skimmed milk power or analysis for soybean powder be respectively 0.6～2.1% or 0.4～1.5% with respect to molasses or its concentration of soya-bean milk, the described concentration that contains in 5% the analysis for soybean powder aqueous solution with sterilized water preparation in sterilization tank is 0.001% pineapple ferment, comprehensive above nutrient source prepares liquid substrate and ferments, in the fermentation cylinder for fermentation temperature is 27～55 ℃, fermentation time is 10～48 hours, when stopping, fermentation adds catabolic enzyme, its addition is to account for 0.01～0.5% of solid-state substrate total amount described in the step c, when adding catabolic enzyme, with ice water circulation the zymocyte liquid temperature is reduced to 7～8 ℃ in the fermentation tank chuck and get final product;
B, the preparation of bacillus coagulans Leehai 887 liquid bioactivators: earlier molasses or soya-bean milk are diluted to sterilized water that to measure concentration with balling hydrometer be 1.5～9brix, under 100 ℃ of conditions, after the sterilization treatment 12～18 minutes, get 3500～4200L and put into fermentation tank, the bacillus coagulans Leehai 887 bacterium liquid 500L that initial stage is cultivated through conventional method pour fermentation tank into again, again in sterilization tank, being the analysis for soybean powder of 8% skimmed milk power or 7% and 1% the anhydrous phosphoric acid dipotassium nutrient source aqueous solution with the concentration of 1000L sterilized water preparation, with 100 ℃, get 300～1000L after 12～18 minutes sterilization treatment and put into fermentation tank, make described skimmed milk power or analysis for soybean powder and anhydrous phosphoric acid dipotassium be respectively 0.7～2.4% or 0.6～2.1% and 0.08～0.3% with respect to molasses or its concentration of soya-bean milk, the described concentration that contains in 7% the analysis for soybean powder aqueous solution with sterilized water preparation in sterilization tank is 0.001% pineapple ferment, comprehensive above nutrient source prepares liquid substrate and ferments, fermentation temperature is 37～42 ℃ during fermentation, fermentation time is 18～48 hours, when stopping, fermentation adds bromelain and alpha-galactosidase, the addition of described bromelain accounts for 0.0005～0.01% of solid-state substrate total amount described in the step c, the addition of described alpha-galactosidase accounts for 0.005～0.05% of solid-state substrate total amount described in the step c, when adding enzyme, with ice water circulation the zymocyte liquid temperature is reduced to 7～8 ℃ in the fermentation tank chuck and get final product;
C, the preparation of bacillus subtilis Tsao 060605 and bacillus coagulans Leehai 887 solid-state bioactivators: earlier with solid-state substrate modified 5～30 minutes with steam, be cooled to 40～43 ℃, then the bacillus subtilis Tsao 060605 liquid bioactivator of step a preparation and the bacillus coagulans Leehai 887 liquid bioactivators of step b preparation are mixed, the shared weight percentage of the liquid bioactivator of bacillus subtilis Tsao060605 is 10～30% during mixing, solid-state substrate and mixed liquid bioactivator after handling are carried out mixed culture fermentation, when solid-state substrate mixes with mixed liquid bioactivator, the shared weight percentage of wherein mixed liquid bioactivator is 25～95%, fermentation temperature is 30～55 ℃ during fermentation, fermentation time is 12～72 hours, finish preceding 0～8 hour adding seawood meal at solid state fermentation, the addition of described seawood meal accounts for 1～10% of solid-state substrate total amount, after the fermentation ends its material is dried under 55～100 ℃ temperature, dry to fermentation substrate moisture≤7%, pulverize at last, obtain the solid state fermentation bioactivator;
D, the preparation of bioactive substance carrier: with step a, it is 20～45% liquid state fermentation bioactivator that the bacillus subtilis Tsao 060605 liquid bioactivator of b preparation and bacillus coagulans Leehai 887 liquid bioactivators are condensed into the dry amount respectively, liquid state fermentation bioactivator after two kinds concentrated mixes, wherein the dry amount is that 20～45% the shared weight percentage of bacillus subtilis Tsao 060605 liquid bioactivator is 40～50%, the dry amount is that 20～45% the shared weight percentage of bacillus coagulans Leehai 887 liquid bioactivators is 50～60%, mixed liquid state fermentation bioactivator is sprayed on the solid state fermentation bioactivator for preparing among the step c, described mixed liquid state fermentation bioactivator sprinkling amount accounts for 0.5～1.5% of solid state fermentation bioactivator total amount, promptly gets bioactive substance carrier after the sprinkling.
2, the preparation method of bioactive substance carrier according to claim 1 is characterized in that: the bacterium number in the bacillus subtilis Tsao 060605 bacterium liquid that conventional method is cultivated of initial stage described in the step a is 〉=1.0 * 10
8CFU/ml, pH-value pH are 5.8～6.5; The bacterium number is 〉=1.2 * 10 in the bacillus subtilis Tsao 060605 bacterium liquid of described fermentation back
7CFU/ml, pH-value pH are 5.8～6.5.
3, the preparation method of bioactive substance carrier according to claim 1 is characterized in that: the granularity of analysis for soybean powder described in the step a is for all to sieve by 40～60 mesh sieve analyses; Described catabolic enzyme is at least a in the poly-candy enzyme of acidic protein catabolic enzyme, bromelain, alphalise starch catabolic enzyme, cellulolytic enzyme, half cellulolytic enzyme, alpha-galactosidase, Xylans enzyme, sweet dew.
4, the preparation method of bioactive substance carrier according to claim 1 is characterized in that: the bacterium number in the bacillus coagulans Leehai 887 bacterium liquid that conventional method is cultivated of initial stage described in the step b is 〉=1.0 * 10
8CFU/ml, pH-value pH are 4.6～5.8; The bacterium number is 〉=1.2 * 10 in the bacillus coagulans Leehai 887 bacterium liquid of fermentation back
7CFU/ml, pH-value pH are 4.3～5.8; The granularity of described analysis for soybean powder is all to sieve by 40～60 mesh sieve analyses.
5, the preparation method of bioactive substance carrier according to claim 1 is characterized in that: at least a in peeling soyabean expeller, analysis for soybean powder, soya bean slag, degrease rice bran, the broad bean dregs of rice, the pea dregs of rice, the cottonseed dregs of rice, rapeseed dregs, wheat bran, barley bran, brewex's grains, maize alcohol stillage, rice vinasse and the corn flour of solid-state substrate described in the step c; Described material is pulverized the back and is crossed 20～40 mesh sieves.
6, a kind of chicken bio-additive premixed feed special, it is characterized in that, described bio-additive premixed feed is to be carrier with the prepared bioactivator of claim 1, and the required raw material of preparation 1000g chicken bio-additive premixed feed special is: bioactive substance carrier 242.0g～397.0g, calcium dihydrogen phosphate 160.0g～267.5g, calcium carbonate 200.0g～280.0g, vitamin(e) supplement 12.5g～30.5g, Amino acid additive 60.0g～72.5g, mineral element 12.5g～25.0g, enzyme preparation 12.5g～20.0g, linolenic acid 80.0g～125.0g, the dry amount is that 20～45% bacillus subtilis Tsao 060607 liquid bioactivator 1.0ml～12.0ml and dry amount are bacillus coagulans Leehai 887 liquid bioactivator 3.0ml～7.8ml of 20～45%.
7, chicken bio-additive premixed feed special according to claim 6 is characterized in that: described preparation 1000g chicken bio-additive premixed feed special also need add mold toxin sorbent 12.5g.
8, chicken bio-additive premixed feed special according to claim 6 is characterized in that: the content of bacillus subtilis Tsao 060605 is 1.0 * 10 in the described bioactive substance carrier
5～3.0 * 10
10CFU/kg, the content of bacillus coagulans Leehai 887 are 1.0 * 10
6～5.0 * 10
11CFU/kg; The content of bacillus subtilis Tsao 060605 is 1.0 * 10 in the described chicken bio-additive premixed feed special
4～2.0 * 10
10CFU/kg, the content of bacillus coagulans Leehai 887 are 1.0 * 10
5～2.0 * 10
11CFU/kg.
9, chicken bio-additive premixed feed special according to claim 6 is characterized in that: described dry amount is that 20～45% bacillus subtilis Tsao 06065 liquid bioactivator is that the bacillus subtilis Tsao 060605 liquid bioactivator that will prepare among the claim 1 step a concentrates according to conventional method and forms; Described dry amount is that 20～45% bacillus coagulans Leehai 887 liquid bioactivators are that the bacillus coagulans Leehai 887 liquid bioactivators that will prepare among the claim 1 step b concentrate according to conventional method and form.
10, chicken bio-additive premixed feed special according to claim 6 is characterized in that: described vitamin(e) supplement, Amino acid additive, mineral element, enzyme preparation and mold toxin sorbent are the national regulation standardized product of selling on the market.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910065923A CN101642198A (en) | 2009-08-25 | 2009-08-25 | Biological additive pre-mixed feed special for chicken and preparation method of biological additive substance carrier thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910065923A CN101642198A (en) | 2009-08-25 | 2009-08-25 | Biological additive pre-mixed feed special for chicken and preparation method of biological additive substance carrier thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101642198A true CN101642198A (en) | 2010-02-10 |
Family
ID=41654380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910065923A Pending CN101642198A (en) | 2009-08-25 | 2009-08-25 | Biological additive pre-mixed feed special for chicken and preparation method of biological additive substance carrier thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101642198A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102417418A (en) * | 2011-09-05 | 2012-04-18 | 麻林涛 | Compound microbial fertilizer special for wheat and a preparation method thereof |
| CN105062916A (en) * | 2015-07-22 | 2015-11-18 | 江南大学 | Application of bacillus coagulans in improving egg production of laying hen |
| CN105494261A (en) * | 2015-12-30 | 2016-04-20 | 天津市寿南山禽蛋营养研发有限公司 | Method and nutrient for producing DHA (Docosahexaenoic Acid) and zinc-rich eggs |
| CN106615927A (en) * | 2016-12-05 | 2017-05-10 | 龚俊勇 | Broiler chicken feed additive based on bacillus coagulans and application thereof |
| CN108660100A (en) * | 2018-07-11 | 2018-10-16 | 金福赛（北京）生物科技有限公司 | Bacillus subtilis and its application in microbial inoculum and broiler fodder |
| CN112048448A (en) * | 2020-07-27 | 2020-12-08 | 天津开发区坤禾生物技术有限公司 | Complex microbial inoculant for reducing ammonia odor of henhouse and preparation method and application thereof |
| CN114601074A (en) * | 2022-04-09 | 2022-06-10 | 河南宏展生物科技有限公司 | Fermented feed and compound feed for laying hens and preparation method of fermented feed and compound feed |
| CN114891690A (en) * | 2022-06-08 | 2022-08-12 | 山东仙普爱瑞科技股份有限公司 | Complex microbial inoculant with growth promoting performance and application thereof |
-
2009
- 2009-08-25 CN CN200910065923A patent/CN101642198A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102417418A (en) * | 2011-09-05 | 2012-04-18 | 麻林涛 | Compound microbial fertilizer special for wheat and a preparation method thereof |
| CN105062916A (en) * | 2015-07-22 | 2015-11-18 | 江南大学 | Application of bacillus coagulans in improving egg production of laying hen |
| CN105062916B (en) * | 2015-07-22 | 2017-12-12 | 江南大学 | Application of one bacillus coagulans on lifting laying hen egg yield |
| CN105494261A (en) * | 2015-12-30 | 2016-04-20 | 天津市寿南山禽蛋营养研发有限公司 | Method and nutrient for producing DHA (Docosahexaenoic Acid) and zinc-rich eggs |
| CN106615927A (en) * | 2016-12-05 | 2017-05-10 | 龚俊勇 | Broiler chicken feed additive based on bacillus coagulans and application thereof |
| CN108660100A (en) * | 2018-07-11 | 2018-10-16 | 金福赛（北京）生物科技有限公司 | Bacillus subtilis and its application in microbial inoculum and broiler fodder |
| CN108660100B (en) * | 2018-07-11 | 2021-12-21 | 金福赛（北京）生物科技有限公司 | Bacillus subtilis and application thereof in microbial inoculum and broiler feed |
| CN112048448A (en) * | 2020-07-27 | 2020-12-08 | 天津开发区坤禾生物技术有限公司 | Complex microbial inoculant for reducing ammonia odor of henhouse and preparation method and application thereof |
| CN114601074A (en) * | 2022-04-09 | 2022-06-10 | 河南宏展生物科技有限公司 | Fermented feed and compound feed for laying hens and preparation method of fermented feed and compound feed |
| CN114891690A (en) * | 2022-06-08 | 2022-08-12 | 山东仙普爱瑞科技股份有限公司 | Complex microbial inoculant with growth promoting performance and application thereof |
| CN114891690B (en) * | 2022-06-08 | 2023-09-08 | 山东仙普爱瑞科技股份有限公司 | Composite microbial inoculant with growth promoting performance and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101828634B (en) | Microbial fermentation antibiotic-free feed and preparing method thereof | |
| CN102934736B (en) | Method for preparing sweet potato skin/ sweet potato powder dreg fermented feed | |
| CN102018098B (en) | Preparation method for fermented feed for corn-bean pulp type daily ration | |
| CN101889628B (en) | Process for producing biosynthesis feed | |
| CN101642198A (en) | Biological additive pre-mixed feed special for chicken and preparation method of biological additive substance carrier thereof | |
| CN101669581A (en) | Preparation method of bioactive single feed | |
| CN102422996B (en) | Preliminary microbial fermentation antibiotic-free feed for growth fattening pigs | |
| CN104054902A (en) | Process for producing fermented soybean meal through solid state fermentation of mixed culture | |
| CN103911323A (en) | Bacillus licheniformis, bacillus subtilis and lactobacillus plantarum preparation and preparation | |
| CN101874546A (en) | Microorganism fermentation feed and preparation method thereof | |
| CN102318779A (en) | Small peptide additive for porcine feed and preparation method thereof | |
| CN101912056B (en) | Method for preparing drug residue free feed for feeding live pigs | |
| CN103184174B (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN101589771A (en) | The preparation method of bio-additive premixed feed special for pigs and bioactive substance carrier thereof | |
| CN105104847A (en) | Fermented feed for fish | |
| CN102517227B (en) | Enterococcus faecalis and applications and feed additive and leavening agent thereof | |
| CN105994941A (en) | Non-antibiotic feed prepared through microbial fermentation | |
| CN104782892A (en) | Bagasse fermented feed and preparation method thereof | |
| CN107821789A (en) | A kind of biologic ferment for improving fishes and shrimps intestinal health degree and its preparation method and application | |
| CN102919557A (en) | Growing fattening pig probiotics composite premix and accessory material | |
| CN102907585A (en) | Probiotics and vitamin premix and mixture for middle pig | |
| CN101717746B (en) | Method for producing spores of clostridium butyricum and application thereof in livestock and poultry rearing | |
| CN105325747A (en) | Special feed additive for laying ducks | |
| CN113508872B (en) | Palm meal raw material biological pretreatment method | |
| CN107691849B (en) | Feed prepared by microbial fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Free format text: FORMER OWNER: ZHOU DONGGEN Owner name: ANHUI LIANG AN BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: CAO JUNPING Effective date: 20110712 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 8/F, NO. 27, LANE 8, SHUIYUAN ROAD, SANMIN DISTRICT, KAOHSIUNG CITY, TAIWAN, CHINA TO: 241000 NO. 30, JIUZI AVENUE, WUHU CITY, ANHUI PROVINCE |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20110712 Address after: 30 No. 241000 Anhui province Wuhu Jiuzi Road Applicant after: Anhui both sides Biotechnology Co., Ltd. Address before: Taiwan, China Kaohsiung District, 8 water source road, No. 27, No. 8, building Applicant before: Cao Junping Co-applicant before: Zhou Donggen |
|
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20100210 |