CN102417418A - Compound microbial fertilizer special for wheat and a preparation method thereof - Google Patents
Compound microbial fertilizer special for wheat and a preparation method thereof Download PDFInfo
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- CN102417418A CN102417418A CN2011102604356A CN201110260435A CN102417418A CN 102417418 A CN102417418 A CN 102417418A CN 2011102604356 A CN2011102604356 A CN 2011102604356A CN 201110260435 A CN201110260435 A CN 201110260435A CN 102417418 A CN102417418 A CN 102417418A
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Abstract
The invention provides a compound microbial fertilizer special for wheat and a preparation method thereof. The compound microbial fertilizer comprises a microbial bacteria liquid and an organic inorganic composite fertilizer, and comprises, by weight, 5-15% of ammonium sulfate, 10-20% of urea, 5-13% of diammonium phosphate, 15-23% of potassium chloride, 35-40% of turf, 5-10% of composite microbial bacteria liquid, 0.2-0.7% of white copperas zinc sulfate, 0.2-0.7% of borax, 0.2-0.7% of ferrous sulphate and 0.2-0.7% of manganese sulfate. The composite microbial bacteria liquid is mixed from new brevibacillus laterosporus and bacillus subtilis bacteria liquid. The special fertilizer can solve problems of excessive chemical fertilizer application, weak pertinency and unbalanced nutrient of an existing fertilizer for wheat, and a problem of unstable application effect of an existing microbial fertilizer, and can increase yield obviously after application.
Description
Technical field
The present invention relates to fertilizer special for wheat material and preparation method thereof, particularly grow wheat specific complex microbial fertilizer and preparation method thereof.
Background technology
Wheat is one of important farm crop, and the many universal composite fertilizers that concentrate with chemical fertilizer such as urea, bicarbonate of ammonia, diammonium phosphate and minority of its fertilising are main, and chemical fertilizer is a quick-acting fertilizer; Short run effect is better; But life-time service is prone to cause soil compaction, destroys soil aggregate.Though and suitable area and crop and the kind wide range of universal composite fertilizer; But for a certain crop; It is superfluous one or both nutrients to occur; And the possible insufficient phenomenon of other nutrient causes the waste of some nutrient, brings the excessive and unbalanced problem of nutrition supply of serious fertilising to Wheat Production.Because these fertilizer are not considered the nutrient quantity that local soil nutrient supply situation and crop absorb and confirm ratio that both the contaminated soil environment limited the yield and quality of crop again; Crop, soil are lacked specific aim, and the fertilizer efficiency utilization ratio is low, and is also big to environmental hazard; Rivers and underground water eutrophication are on the rise, and soil organic matter content reduces, and the beneficial microorganism living space diminishes in the soil; Quantity reduces, and disease-resistant, cold-resistant, the drought-resistant ability of farm crop reduce, and the generation of disease and pest is more and more serious; Cause wheat quality to descend, output reduces.
Microbial fertilizer more and more is familiar with by people as a kind of important effect of agrotechnical measure in the development an agriculture featuring high yields, fine quality and high efficiency; This provides sizable development space for its exploitation, popularization, application; Particularly the irreplaceability in development Organic farming, production A level, AA level green food has demonstrated fully its potential glamour.But the problem of microbial fertilizer existence at present is that effective bacterium number is not up to standard, the quality guaranteed period is too short, thereby effect is unstable.
In the prior art also not with the report of microbial fertilizer as fertilizer specially for wheat.
Summary of the invention
The present invention exists chemical fertilizer to use excessive, problems such as specific aim is poor, nutrient is unbalanced, existing microbial fertilizer application effect instability in order to solve the existing fertilizer that is used for wheat, and a kind of NEW TYPE OF COMPOSITE mikrobe fertilizer specially for wheat is provided.Obviously increasing both production and income after using.
In the present invention, if not refer in particular to, all part, per-cents are weight unit, and all equipment and starting material etc. all can be buied from market or the industry is commonly used.
The present invention adopts following technical scheme to realize:
Wheat specific complex microbial fertilizer of the present invention comprises composite microbial bacteria liquid and composite organic-inorganic fertilizer, by weight percentage: ammonium sulfate 5-15%, urea 10-20%; Diammonium phosphate 5-13%, Repone K 15-23%, peat composed of rotten mosses 35-40%; Composite microbial bacteria liquid 5-10%, zinc sulfate 0.2-0.7%, borax 0.2-0.7%; Ferrous sulfate 0.2-0.7%, manganous sulfate 0.2-0.7% adds up to 100%.
Described composite microbial bacteria liquid makes microbial inoculum through fermentation respectively by bacillus laterosporus (Bacillus laterosporus) and subtilis (Bacillus subtilis), mixes according to arbitrary proportion again.
Preferably, the mixed weight ratio of bacillus laterosporus and subtilis is 1-10:1-10, is more preferably 1:1.
The present invention adopts new bacillus laterosporus (Bacillus laterosporus), specifically is named as " new No. 1 bacterial strain of lateral bud spore bacillus ", and preserving number is CGMCC No.5090.This strain separating is from high-yielding grain fields, and through domestication, proterties is stable for a long time.
The biological characteristics of this bacterial classification is that thalline is shaft-like, belongs to bacillus, and its cell size is 0.15～0.18 * 215～510um; Gram-positive; Can be changed into feminine gender, gemma is oval, adnation, middle life or nearly middle giving birth to; Sporangiocyst expands, and free gemma is on one side than the other side thick (canoe shape).Can utilize multiple nitrogenous source, carbon source, growth temperature 20-55 ℃, 37 ℃ of optimum growth temps, optimal pH 7.2.Under bad condition, thalline transforms and produces gemma, and gemma can tolerate mal-conditions such as 100 ℃ of high temperature 20 minutes and drying to the poor environment strong stress resistance.
New No. 1 bacterial strain of lateral bud spore bacillus is submitted China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation on July 22nd, 2010; Deposit number is CGMCC No.5090; Biology name Bacillus laterosporus; Depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
The present invention adopts new subtilis (Bacillus subtilis), specifically is named as " new No. 1 bacterial strain of subtilis ", and preserving number is CGMCC No.5091.This strain separating is from high-yielding grain fields, and through domestication, proterties is stable for a long time.
The biological characteristics of this bacterial classification is that thalline is shaft-like, the gemma aerophil of making a living, and its gemma can resist high heat (still can survive in following 30 minutes at 100 ℃), and can in the withered grass leach liquor, grow, and its gemma is ellipse or long barrel shape, 0.6～0.9 * 1.0～1.5 μ m.Form in sporangium central authorities or nearly central part, generally between waist, germinate.Shaft-like 0.3～0.7 * 2～3 μ m of vegetative cell, isolated or one-tenth short chain, rod end is semicircle.Bacterium colony is spreading property, and scabriculous or folding are greyish white, and be yellowish even yellow.When growing in the liquid medium within, the normal wrinkle mould that forms.Aerophil.Available protein, multiple sugar and starch decompose tryptophane and form indoles.Growth temperature 20-55 ℃, 37 ℃ of optimum growth temps, optimal pH 7.1.Under bad condition, thalline transforms and produces gemma, and gemma can tolerate mal-conditions such as 100 ℃ of high temperature 15 minutes and drying to the poor environment strong stress resistance.
New No. 1 bacterial strain of subtilis is submitted China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation on July 22nd, 2010; Deposit number is CGMCC No.5091; Biology name Bacillus subtilis; Depositary institution address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
Further, the prescription of wheat specific complex microorganism fertilizer of the present invention does, by weight percentage: ammonium sulfate 10%, urea 15%; Diammonium phosphate 8%, Repone K 18%, the peat composed of rotten mosses 39%, composite microbial bacteria liquid 8%; Zinc sulfate 0.5%, borax 0.5%, ferrous sulfate 0.5%, manganous sulfate 0.5%.
In the present invention, said composite microbial bacteria liquid is prepared from following new cultural method:
1) bacillus laterosporus CGMCC No.5090 or subtilis CGMCC No.5091 bacterial strain are rule respectively insert slant medium (new 1 substratum), 30-38 ℃ of incubators left standstill dark condition under cultivation 36 hours;
2) use the aseptic water washing inclined-plane, form bacteria suspension;
3) 1 volume bacteria suspension is inserted in new 1 liquid nutrient medium of 20 volumes, it is 10 that 30-38 ℃ of aerobics are cultured to cell concentration
8Individual/the ml order of magnitude;
4) bacteria suspension that step 3) is obtained joins in new 2 substratum of 5-10 times of volume, and it is 10 that 30-38 ℃ of aerobics are cultured to cell concentration
8Individual/the ml order of magnitude;
5) bacteria suspension that step 4) is obtained joins in new 3 substratum of 50-100 times of volume, and it is 10 that 35-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude;
6) bacteria suspension that step 5) is obtained joins in new 4 substratum of 5-10 times of volume, and it is 10 that 35-38 ℃ of cultivation aerobic is cultured to cell concentration
9Individual/the ml order of magnitude, stop fermentation;
Wherein above-mentioned substratum is: new No. 1 substratum (slant medium): peptone 5g/L, and sodium-chlor 5g/L, Carnis Bovis seu Bubali cream 5g/L, agar 1.5%-2.0%, PH transfers to 6.5-7.8,
New No. 1 liquid nutrient medium: this substratum does not contain agar, and other compositions are identical with new 1 substratum with content, and PH transfers to 6.5-7.8,
New No. 2 substratum: starch 5g/L, sucrose 1g/L, Yeast diffusion juice 0.2g/L, peptone 5g/L, Carnis Bovis seu Bubali cream 5g/L, ammonium sulfate 1g/L, potassium primary phosphate 0.5g/L, sal epsom 0.2g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8,
New No. 3 substratum: starch 10g/L, sucrose 5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 10g/L, ammonium sulfate 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8,
New No. 4 substratum: starch 10g/L, sucrose 2.5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 5g/L, ammonium sulfate 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8.
The side spore bacillus brevis that fermentation is obtained mixes with any weight ratio with subtilis liquid and obtains composite microbial bacteria liquid, and this bacterium liquid is adsorbed high-quality peat composed of rotten mosses powder (commercially available) through the dry sterilization postcooling, and to obtain complex micro organism fungicide subsequent use.Preferably, the mixed weight ratio of bacillus laterosporus and subtilis is 1-10:1-10, is more preferably 1:1.
Again with all the other siccatives pulverize respectively, proportional mixing, add the above-mentioned complex micro organism fungicide of processing through fermentation absorption, mixing and through oven drying at low temperature, granulation, pack makes wheat specific complex microbial fertilizer of the present invention.
Total nutrient content (N+P in the said wheat specific complex microorganism fertilizer
2O
5+ K
2O)>=25%, organic>=25%, living bacteria count (individual/gram)>=2.0 * 10
7, special-purpose fertile physical behavior that obtains with this understanding and proportion, fertilizer efficiency etc. are for best.
Below in conjunction with embodiment the present invention is specifically described, the present invention also can realize through other embodiments that does not break away from its spirit or essential feature.Therefore, below embodiment, just illustrate, be not only, all are within the scope of the present invention or be equal to change in the scope of the present invention and all comprised by the present invention.
Embodiment:
Embodiment 1:
One grow wheat specific complex microorganism fertilizer, its prescription do, by weight percentage: ammonium sulfate 10%, urea 15%, diammonium phosphate 8%, Repone K 18%, the peat composed of rotten mosses 39%, composite microbial bacteria liquid 8%, zinc sulfate 0.5%, borax 0.5%, ferrous sulfate 0.5%, manganous sulfate 0.5%.
Described composite microbial bacteria liquid is by bacillus laterosporus (Bacillus laterosporus); Preserving number is CGMCC No.5090; And subtilis (Bacillus subtilis); Preserving number is CGMCC No.5091, makes microbial inoculum through fermentation respectively, forms after the part by weight mixing according to 1:1 again.
The preparation technology of bacillus laterosporus bacterium liquid is following:
Earlier the bacillus laterosporus bacterial classification is carried out activation, use 5ml aseptic water washing inclined-plane again, form bacteria suspension; Bacteria suspension inserts the 500ml triangular flask, and this triangular flask contains new 1 liquid nutrient medium of 100ml, uses shaking table to cultivate 18 hours; Culture condition is 37 ℃, and 180-200rpm detects cell concentration; Reach 108 orders of magnitude in concentration, carry out next step;
100ml bacterium liquid in the 500ml triangular flask is transferred in the 2000ml triangular flask, and this triangular flask contains new 2 substratum of 700ml, uses shaking table to cultivate 20 hours, and culture condition is 37 ℃, 180-200rpm; Detect cell concentration, reach 108 orders of magnitude, carry out next step in concentration;
Bacterium liquid in the 2000ml triangular flask is inserted in the 1m3 fermentor tank, and the access amount is 6 liters, contains new 3 substratum of 500L in this fermentor tank, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 20m3/h cultivated 8 hours; Detect cell concentration, reach 108 orders of magnitude, carry out next step in concentration;
Bacterium liquid in the 1m3 fermentor tank is inserted the 5m3 fermentor tank, and the access amount is 500 liters, and this fermentor tank contains new 4 substratum of 3m3, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 120m3/h cultivated 15 hours.Detect cell concentration, reach 109 orders of magnitude, stop fermentation in concentration.
The preparation technology of subtilis bacterium liquid is following:
Earlier Bacillus subtilis strain is carried out activation, use 5ml aseptic water washing inclined-plane again, form bacteria suspension; Bacteria suspension inserts the 500ml triangular flask, and this triangular flask contains new 1 liquid nutrient medium of 100ml, uses shaking table to cultivate 18 hours; Culture condition is 37 ℃, and 180-200rpm detects cell concentration; Reach 108 orders of magnitude in concentration, carry out next step;
100ml bacterium liquid in the 500ml triangular flask is transferred in the 2000ml triangular flask, and this triangular flask contains new 2 substratum of 700ml, uses shaking table to cultivate 20 hours, and culture condition is 37 ℃, 180-200rpm; Detect cell concentration, reach 108 orders of magnitude, carry out next step in concentration;
Bacterium liquid in the 2000ml triangular flask is inserted in the 1m3 fermentor tank, and the access amount is 6 liters, contains new 3 substratum of 500L in this fermentor tank, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 20m3/h cultivated 8 hours; Detect cell concentration, reach 108 orders of magnitude, carry out next step in concentration;
Bacterium liquid in the 1m3 fermentor tank is inserted the 5m3 fermentor tank, and the access amount is 500 liters, and this fermentor tank contains new 4 substratum of 3m3, and fermentation condition is: temperature 36-38 ℃, and tank pressure 0.5kg/cm, air flow 120m3/h cultivated 15 hours.Detect cell concentration, reach 109 orders of magnitude, stop fermentation in concentration.
The side spore bacillus brevis liquid that above-mentioned fermentation is obtained mixes with the 1:1 weight ratio with subtilis liquid and obtains composite microbial bacteria liquid, and this bacterium liquid is adsorbed high-quality peat composed of rotten mosses powder (commercially available) through the dry sterilization postcooling, and to obtain complex micro organism fungicide subsequent use.
Again all the other siccatives are pulverized, mixed, adds and above-mentionedly adsorb the complex micro organism fungicide of processing through fermentation, mixing and through oven drying at low temperature, granulation, pack makes wheat specific complex microbial fertilizer of the present invention.
Embodiment 2
One grow wheat specific complex microorganism fertilizer, its prescription do, by weight percentage: ammonium sulfate 15%, urea 10%; Diammonium phosphate 5%, Repone K 18%, the peat composed of rotten mosses 40%, composite microbial bacteria liquid 10%; Zinc sulfate 0.5%, borax 0.5%, ferrous sulfate 0.5%, manganous sulfate 0.5%.
Described composite microbial bacteria liquid is by bacillus laterosporus (Bacillus laterosporus); Preserving number is CGMCC No.5090; And subtilis (Bacillus subtilis); Preserving number is CGMCC No.5091, makes microbial inoculum through fermentation respectively, forms after the part by weight mixing according to 1:2 again.
The preparation technology of bacillus laterosporus bacterium liquid and subtilis liquid is with embodiment 1.
The side spore bacillus brevis liquid that above-mentioned fermentation is obtained mixes with the weight ratio of 1:2 with subtilis liquid and obtains composite microbial bacteria liquid, and this bacterium liquid is adsorbed high-quality peat composed of rotten mosses powder (commercially available) through the dry sterilization postcooling, and to obtain complex micro organism fungicide subsequent use.
Again all the other siccatives are pulverized, mixed, adds and above-mentionedly adsorb the complex micro organism fungicide of processing through fermentation, mixing and through oven drying at low temperature, granulation, pack makes wheat specific complex microbial fertilizer of the present invention.
Embodiment 3
One grow wheat specific complex microorganism fertilizer, its prescription do, by weight percentage: ammonium sulfate 5%, urea 20%, diammonium phosphate 5%, Repone K 23%, the peat composed of rotten mosses 35%, composite microbial bacteria liquid 10%, zinc sulfate 0.5%, borax 0.5%, ferrous sulfate 0.5%, manganous sulfate 0.5%.
Described composite microbial bacteria liquid is by bacillus laterosporus (Bacillus laterosporus); Preserving number is CGMCC No.5090; And subtilis (Bacillus subtilis); Preserving number is CGMCC No.5091, makes microbial inoculum through fermentation respectively, forms after the part by weight mixing according to 2:1 again.
The preparation technology of bacillus laterosporus bacterium liquid and subtilis liquid is with embodiment 1.
The side spore bacillus brevis liquid that above-mentioned fermentation is obtained mixes with the weight ratio of 2:1 with subtilis liquid and obtains composite microbial bacteria liquid, and this bacterium liquid is adsorbed high-quality peat composed of rotten mosses powder (commercially available) through the dry sterilization postcooling, and to obtain complex micro organism fungicide subsequent use.
Again all the other siccatives are pulverized, mixed, adds and above-mentionedly adsorb the complex micro organism fungicide of processing through fermentation, mixing and through oven drying at low temperature, granulation, pack makes wheat specific complex microbial fertilizer of the present invention.
The manure trial of the present invention's " wheat specific complex microbial fertilizer " on wheat:
1. test materials and method
Test being arranged in Wang Ji township, Xinye County, Nanyang in April, 2009 and collecting, supplying examination soil is the loess loam, and physical features is smooth, and fertility is even.This piece topsoil soils basis, field nutrient is: organic 9.45g/kg.Confession studies the thing wheat, and kind is the exhibition 4110 of laying down.Supply examination fertilizer: wheat specific complex microbial fertilizer of the present invention, Comparative Examples: conventional composite fertilizer.
2 processing are established in this test, repeat sub-district area 66.7m2, sub-district random alignment 3 times.
Handle 1 (the present invention): mu is executed the 40kg embodiment of the invention 1 wheat specific complex microbial fertilizer;
Handle 2 (simultaneous tests): mu is executed the conventional compound manure of 50kg.
This is tested in whole ground on October 12nd, 2008, fertilizer bottom application, sowing on October 23, the mu amount of broadcasting 7kg.Growing period waters 2 times, prevention and elimination of disease and pests 1 time.The single meter of receiving in sub-district produces during results, and picked at random 1m2 plant does indoor species test, and control measures are with local high yield land for growing field crops standard.
2. test-results and analysis
2.1 execute wheat specific complex microbial fertilizer of the present invention becomes the product factor to wheat influence
Use wheat specific complex microbial fertilizer of the present invention and improved the Wheat Production factor.Can be found out by table 1: the thousand grain weight of wheat of using wheat specific complex microbial fertilizer of the present invention is than handling 2 high 2.6 grams, and mu fringe Lou and grain number per spike change little.
Species test of table 1 wheat and investigation record table
| Handle | Mu spike number (ten thousand) | Grain number per spike (individual) | Thousand seed weight (g) | Theoretical yield (kg) |
| 1 | 28.6 | 31.8 | 43.6 | 396.5 |
| 2 | 28.6 | 31 | 41 | 362.2 |
2.2 use the influence of wheat specific complex microbial fertilizer of the present invention to wheat yield
Table 2 is respectively handled cell production cartogram as a result
Use the wheat yield of wheat specific complex microbial fertilizer of the present invention and handle 2 raising the output 39kg/ mus, stimulation ratio 13.1%.
Looking into t value table knows: when n=4, and t
0.05=2.78, t
0.01=4.60, actual t value=8.66>t
0.01=4.60, difference is extremely remarkable.
3. effect
Wheat specific complex microorganism fertilizer of the present invention has added new microbial strains; And the cultivation and fermentation method through new prepares new microbiobacterial agent, and then itself and composite fertilizer be mixed with form, and can find out that through test wheat applies this special use fertilizer and can roll up thousand seed weight; Thereby significantly increase output; Reduce fertilizer amount, effect stability, effective is worthy of popularization.
Claims (5)
1. a grow wheat specific complex microbial fertilizer comprises composite microbial bacteria liquid and composite organic-inorganic fertilizer, it is characterized in that consisting of by weight percentage: ammonium sulfate 5-15%, urea 10-20%; Diammonium phosphate 5-13%, Repone K 15-23%, peat composed of rotten mosses 35-40%; Composite microbial bacteria liquid 5-10%, zinc sulfate 0.2-0.7%, borax 0.2-0.7%; Ferrous sulfate 0.2-0.7%, manganous sulfate 0.2-0.7% adds up to 100%; Described composite microbial bacteria liquid is that CGMCC No.5090 and subtilis (Bacillus subtilis), preserving number are that CGMCC No.5091 makes microbial inoculum through fermentation respectively by bacillus laterosporus (Bacillus laterosporus), preserving number, mixes according to arbitrary proportion again.
2. wheat specific complex microbial fertilizer as claimed in claim 1, by weight percentage: ammonium sulfate 10%, urea 15%, diammonium phosphate 8%; Repone K 18%, the peat composed of rotten mosses 39%, composite microbial bacteria liquid 8%, zinc sulfate 0.5%; Borax 0.5%, ferrous sulfate 0.5%, manganous sulfate 0.5%.
3. according to claim 1 or claim 2 wheat specific complex microbial fertilizer, wherein the mixed weight ratio of composite microbial bacteria liquid bacillus laterosporus and subtilis is 1-10:1-10.
4. according to claim 1 or claim 2 wheat specific complex microbial fertilizer, wherein the mixed weight ratio of composite microbial bacteria liquid bacillus laterosporus and subtilis is 1:1.
5. like the preparation method of the described wheat specific complex of claim 1-4 microbial fertilizer, it is characterized in that comprising the steps:
The first step: preparation microbial inoculum:
1) bacillus laterosporus CGMCC No.5090 or subtilis CGMCC No.5091 bacterial strain are rule respectively insert slant medium (new 1 substratum), 30-38 ℃ of incubators left standstill dark condition under cultivation 36 hours;
2) use the aseptic water washing inclined-plane, form bacteria suspension;
3) 1 volume bacteria suspension is inserted in new 1 liquid nutrient medium of 20 volumes, it is 10 that 30-38 ℃ of aerobics are cultured to cell concentration
8Individual/the ml order of magnitude;
4) bacteria suspension that step 3) is obtained joins in new 2 substratum of 5-10 times of volume, and it is 10 that 30-38 ℃ of aerobics are cultured to cell concentration
8Individual/the ml order of magnitude;
5) bacteria suspension that step 4) is obtained joins in new 3 substratum of 50-100 times of volume, and it is 10 that 35-38 ℃ of aerobic is cultured to cell concentration
8Individual/the ml order of magnitude;
6) bacteria suspension that step 5) is obtained joins in new 4 substratum of 5-10 times of volume, and it is 10 that 35-38 ℃ of cultivation aerobic is cultured to cell concentration
9Individual/the ml order of magnitude, stop fermentation;
Wherein above-mentioned substratum is: new No. 1 substratum (slant medium): peptone 5g/L, and sodium-chlor 5g/L, Carnis Bovis seu Bubali cream 5g/L, agar 1.5%-2.0%, PH transfers to 6.5-7.8,
New No. 1 liquid nutrient medium: this substratum does not contain agar, and other compositions are identical with new 1 substratum with content, and PH transfers to 6.5-7.8,
New No. 2 substratum: starch 5g/L, sucrose 1g/L, Yeast diffusion juice 0.2g/L, peptone 5g/L, Carnis Bovis seu Bubali cream 5g/L, ammonium sulfate 1g/L, potassium primary phosphate 0.5g/L, sal epsom 0.2g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8,
New No. 3 substratum: starch 10g/L, sucrose 5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 10g/L, ammonium sulfate 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8,
New No. 4 substratum: starch 10g/L, sucrose 2.5g/L, Yeast diffusion juice 0.1g/L, peptone 0.5g/L, soybean cake powder 5g/L, ammonium sulfate 1g/L, potassium primary phosphate 0.5g/L, sodium-chlor 5g/L, PH transfers to 6.5-7.8;
Second step: will ferment the side spore bacillus brevis liquid that obtains and subtilis liquid mix with said weight ratio and obtain composite microbial bacteria liquid, and this bacterium liquid is adsorbed high-quality peat composed of rotten mosses powder (commercially available) through the dry sterilization postcooling, and to obtain complex micro organism fungicide subsequent use;
The 3rd step: again with all the other siccative ammonium sulfate, urea, diammonium phosphate, Repone K, zinc sulfate, borax, ferrous sulfate with manganous sulfate is pulverized respectively, proportional mixing; Add the complex micro organism fungicide that second step processed through fermentation absorption, mixing also promptly gets through oven drying at low temperature, granulation, pack.
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