CN110050890A - A kind of beef cattle fermenting feed and its preparation method and application method - Google Patents
A kind of beef cattle fermenting feed and its preparation method and application method Download PDFInfo
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- CN110050890A CN110050890A CN201910404524.XA CN201910404524A CN110050890A CN 110050890 A CN110050890 A CN 110050890A CN 201910404524 A CN201910404524 A CN 201910404524A CN 110050890 A CN110050890 A CN 110050890A
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- beef cattle
- feed
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- fermentation
- lactic acid
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Birds (AREA)
- Sustainable Development (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of beef cattle fermenting feed and its preparation method and application methods.Beef cattle fermenting feed includes the lactic acid bacteria metabolite and yeast culture that mass ratio is 6-7:3-4；Lactic Acid Bacteria Metabolites include the raw material of following parts by weight: I and 450-500 parts of 950-1000 parts of feedstuffs, 0.008-0.01 parts of lactobacillus plantarum bacterium powder, 1-1.5 parts of complex enzyme formulations water；Yeast culture includes the raw material of following parts by weight: 950-1000 parts of fermentation raw materials, II, 4.75-10 parts of S. cervisiae powder of 14.25-20 parts of complex enzyme formulations, 545-600 parts of water；Preparation method are as follows: preparation lactic acid bacteria metabolite；Prepare yeast culture；Prepare fermented feed.Beef cattle fermenting feed of the invention has the advantages that attractant is good, beef cattle growth, digestibility can be promoted high.
Description
Technical field
The present invention relates to fermented feed technical field, more specifically, it relates to a kind of beef cattle fermenting feed and its
Preparation method and application method.
Background technique
Fermented feed is using feedstuff and feed addictive as object, using bioengineering, enzyme engineering, Fermentation Engineering etc.
New and high technology, novel fodder resource made of exploitation.Biological fermentation feed has small-molecular peptides, high activity metabolite, enzyme system
The substances such as agent, oligosaccharide, B family vitamin can remarkably promote animal digestion and absorb, improve breeding environment, improve animal product
Quality, application prospect are extensive.
That there are recipe ingredients is unreasonable for the existing beef cattle fermented feed in market, and finished product pH value is relatively low, easily causes beef cattle rumenic acid
The problems such as poisoning, finished product smell is bad, and attractant is poor, and digestibility is low, seriously affects its using effect.Moreover, if beef cattle is raised
After feeding ensilage, continue the biggish fermented feed of feeding moisture, beef cattle growth indexes are promoted with meat improvement not
Obviously.
The high novel beef cattle fermenting feed of good, promotion beef cattle growth that therefore, it is necessary to a kind of attractants, digestibility, with
Meets the needs of raiser.
Summary of the invention
In view of the deficienciess of the prior art, the first purpose of this invention is to provide a kind of beef cattle fermenting feeding
Material has the advantages that attractant is good, beef cattle growth, digestibility can be promoted high.
Second object of the present invention is to provide a kind of preparation method of beef cattle fermenting feed, can prepare
Applicability is universal, obvious economic, the fermented feed that can stablize Rumen Internal Environment.
Third object of the present invention is to provide a kind of application method of beef cattle fermenting feed, with applicability
Generally, the advantages of beef cattle capable of being promoted to grow.
To realize above-mentioned first purpose, the present invention provides the following technical scheme that a kind of beef cattle fermenting feed, packet
Include the lactic acid bacteria metabolite and yeast culture that mass ratio is 6-7:3-4；
The Lactic Acid Bacteria Metabolites include the raw material of following parts by weight: 950-1000 parts of feedstuffs, 0.008-0.01 portions of plants
I and 450-500 parts of lactobacillus bacterium powder, 1-1.5 parts of complex enzyme formulations water；
The yeast culture includes the raw material of following parts by weight: 950-1000 parts of fermentation raw materials, 14.25-20 parts of complex enzyme systems
II, 4.75-10 parts of S. cervisiae powder of agent, 545-600 parts of water.
By using above-mentioned technical proposal, due to preparing the dedicated hair of beef cattle using lactic acid bacteria metabolite and yeast culture
Ferment feed, the lactobacillus plantarum bacterium powder wherein contained in lactic acid bacteria metabolite have and maintain intestinal flora balance, improve
Immunity of organisms and the function of promoting absorption of nutrient ingredients, while by enzymolysis, it can effectively maintain pH in beef cattle cud
Value promotes digestion and absorption of the beef cattle to fermented feed, improves efficiency of feed utilization, and complex enzyme formulation mutually cooperates with work using a variety of enzymes
With can play the effect of enzyme preparation to greatest extent, promote the digestion and absorption of fermented feed；S. cervisiae powder can be controlled
Indigestion is treated, and makes fermented feed that there is mellowness, is formulated rationally by mixed Lactic Acid Bacteria Metabolites and Yeast culture,
Rich in multiple nutritional components, such as lactic acid bacteria metabolite, metabolism of yeasts product, oligopeptides, organic acid, reduced sugar, aromatics
Object, unknown growth factor etc. can adjust the Bacterial community in beef cattle cud, promote beef cattle growth, shorten the fattening period, simultaneously
Because the feed after fermentation has sour and aroma, attractant is preferable.
Further, the feedstuff includes the raw material of following weight percent: corn 35-40%, wheat bran 2-5%,
Dregs of beans 4-6%, cotton dregs 10-12%, maize peel 11-15%, alfalfa meal 1-2%, corn germ cake 12-15%, vinasse albumen are raised
Expect 16-20%.
By using above-mentioned technical proposal, since corn is the grain trough of a kind of high starch, high thermal energy, low-protein,
Dry matter content is 88% or so, and crude protein content about 9%, nutrient matrix is high, and palatability is good, and digestibility is high；The starch of wheat bran
Content is higher, arranges in pairs or groups and uses with corn, can promote enterogastric peristalsis, and alfalfa meal is rich in amino acid, minerals and microelement, closes
Reason incorporation alfalfa meal, can accelerate the growth of beef cattle, improve the immunocompetence of beef cattle；Protein content is up to 30-50% in dregs of beans,
It is the main protein feed resource of beef cattle, the dregs of beans through everfermentation, can generate largely has unique physiological active functions
Active peptide, active peptide is easy to digest, absorption is fast, antigenicity is low, and the beneficial bacterium proliferation in energy effective stimulus enteron aisle is adjusted in beef cattle body
The structure of Tiny ecosystem flora increases entire alimentary canal to the decomposition of feed nutrition substance, synthesis, absorption and utilization, and in dregs of beans
Containing multiple-microorganism enzyme, such as protease, amylase.Lipase etc. can supplement enzyme source deficiency, reinforced nutrition matter in matrix
Digestion, improve beef cattle to the utilization rate of feed protein and energy, dregs of beans has special fermenting aroma, and palatability is good, energy
Beef cattle gut pH is adjusted, the metabolism of body is participated in, promotes growth；In vinasse albumen feed due to the role of microorganisms,
Protein, B family vitamin and the good height of amino acid content, and containing the unknown somatomedin generated in fermentation, it is beef cattle growth
Required protein, fat, vitamin and mineral good source, nutritive value is high, can improve the quality of beef cattle, reduces and supports
Cost is grown, beef cattle fast-growth is promoted.
Further, the fermentation raw material includes the raw material of following weight percent: corn 75-80%, wheat bran 20-
25%.
By using above-mentioned technical proposal, corn is the grain trough of a kind of high starch, high thermal energy, low-protein, dry
Matter content 88% or so, crude protein content about 9%, nutrient matrix is high, and palatability is good, and digestibility is high；The content of starch of wheat bran compared with
Height is arranged in pairs or groups with corn and is used, can promote enterogastric peristalsis, and the speed of growth of beef cattle is promoted, and improves immunocompetence.
Further, alkali protease, neutral proteinase and acidity that the complex enzyme formulation I is 7:2:1 by mass ratio
Protease composition, the enzyme activity of alkali protease are 200000u/g, and the enzyme activity of neutral proteinase is 100000u/g, acid egg
The enzyme activity of white enzyme is 20000u/g.
By using above-mentioned technical proposal, alkali protease energy aminosal is changed complicated macromolecular protein structures and is become
At simple small-molecular peptides or amino acid, thus make feed become be easier to absorb, neutral proteinase can be by high molecular weight protein
Matter is hydrolyzed to the products such as amino acid, is conducive to the effective absorption and utilization of protein, reduces feeding cost, acid protease can
Efficiency of feed utilization is improved, using acid, alkali and neutral proteinase, the utilization rate of protease can be improved, adapts to point under varying environment
Solution.
Further, the complex enzyme formulation II is made of the carbohydrase and alpha-amylase that mass ratio is 2:1, carbohydrase
Enzyme activity is 100,000 u/g, and the enzyme activity of alpha-amylase is 5000u/g.
By using above-mentioned technical proposal, carbohydrase can be glucose Starch Hydrolysis, can reduce or eliminate feed
In anti-nutritional factors, improve intestinal flora distribution, promote nutriment and gastral contact and absorptivity, alpha-amylase
Its product for hydrolyzing starch is mainly the maltose and some oligosaccharide and a small amount of glucose of high-content, can improve beef cattle
Daily gain and feed conversion rate.
Further, the bacterium amount of the lactobacillus plantarum bacterium powder is 400,000,000,000/g, and the bacterium amount of the S. cervisiae powder is
20000000000/g.
By using above-mentioned technical proposal, lactobacillus plantarum can maintain beef cattle intestinal flora to balance, and improve immunity of organism
Power and promotion absorption of nutrient ingredients, S. cervisiae powder contain several physiological active substances and nutritional ingredient, can not only improve
The nutrient matrix of feed, moreover it is possible to body is treated because of disease caused by indigestion or hypovitaminosis, reduces the illness rate of beef cattle,
Digestibility of the beef cattle to cellulose, protein etc. in feed is improved, strengthen immunity improves the utilization rate of feed, promotes beef cattle
Growth.
To realize above-mentioned second purpose, the present invention provides the following technical scheme that a kind of beef cattle fermenting feed
Preparation method, comprising the following steps:
It prepares lactic acid bacteria metabolite: lactobacillus plantarum bacterium powder, complex enzyme formulation I and water being added into feedstuff, mixing is equal
After even, the anaerobic fermentation 60-72h at 38-40 DEG C is after fermentation, dry, controls moisture content of finished products≤12%, crushes, cream is made
Sour bacterium metabolite；
Wherein feedstuff is by corn, wheat bran, dregs of beans, cotton dregs, maize peel, alfalfa meal, corn germ cake and vinasse albumen feed
It is mixed according to a certain percentage；
It prepares yeast culture: complex enzyme formulation II and water being added into fermentation raw material, 8-10h is digested at 65-70 DEG C to enzyme
Completely, saccharomyces cerevisiae powder and remaining water, after mixing, aerobic fermentation 48-60h, fermentation ends at 30-32 DEG C is added in solution
Afterwards, dry, moisture content of finished products≤12% is controlled, is crushed, yeast culture obtained；
Wherein material issuance is mixed according to a certain percentage by corn and wheat bran, high-temperature sterilization, cooling is made；
Fermented feed: lactic acid bacteria metabolite and yeast culture are proportionally uniformly mixed, fermented feed is obtained.
By using above-mentioned technical proposal, feedstuff is fermented with lactobacillus plantarum bacterium powder, complex enzyme formulation, and
Drying makes moisture≤12% after fermentation, and lactic acid bacteria metabolite obtained is adjustable the immunity of body, improves the meat of beef cattle,
By fermentation raw material and complex enzyme formulation II mixed enzymolysis, make in fermentation raw material protein digestion at amino acid, when enzymatic hydrolysis completely after,
Saccharomyces cerevisiae powder is added, by fermentation raw material aerobic fermentation, makes fermentation raw material that there is special mellow, by lactic acid bacteria generation obtained
Product and yeast culture mixing are thanked, fermented feed moisture content obtained is few, can improve the meat of beef cattle, and palatability is good,
Attractant is splendid, beef cattle can be promoted to grow, and shortens the beef ox fattening phase.
Further, when preparing lactic acid bacteria metabolite, using aeration-drying mode, drying temperature is 80-95 DEG C, preparation
When yeast culture, using aeration-drying mode, drying temperature is 60-65 DEG C.
By using above-mentioned technical proposal, lactic acid bacteria metabolite and yeast culture are carried out using aeration-drying mode
It is dry, cost can be reduced, energy consumption, and dry gentle uniform, no dead angle are reduced.
Further, when preparing yeast culture, in fermentation process when material temperature is more than 35 DEG C, material is worked as in stirring cooling
When temperature is not up to 35 DEG C, every 10-12h stirring is primary.
By using above-mentioned technical proposal, when temperature is more than 35 DEG C, temperature is excessively high, can cause the too fast life of microorganism
Long, the nutriment in fermentation system is excessively consumed, so that fermentation cannot proceed normally, when temperature is not up to 35 DEG C, is turned over
Dynamic fermentation material can make fermentation raw material fermentation thorough.
To realize above-mentioned third purpose, the present invention provides the following technical scheme that a kind of beef cattle fermenting feed
Application method, suitable for each stage beef cattle after weaning, adding proportion is fattening 50~80g/ early period head/day, fattens mid-term 80
~120g/ head/day fattens 120~150g/ of later period head/day.
Fermented feed is rationally added, can promote beef cattle in each stage of cattle feeding by using above-mentioned technical proposal
Growth improves beef cattle immunity, reduces disease incidence and death and culling rate
In conclusion the invention has the following advantages:
The first, since the present invention can be effective using the metabolism of microorganism using complex enzyme formulation I fermented feed raw material
The crude fibre reduced in feedstuff, the content of the nutritional ingredients such as protein is improved, simultaneously because the growth course of microorganism
It is middle to generate the bioactive substances such as a large amount of amino acid, vitamin, the nutrient matrix of fermented feed can be improved, and pass through plant
The fermentation of lactobacillus bacterium powder, can improve the Tiny ecosystem of stomach, improve the immunocompetence of body, and S. cervisiae is added, can increase
Add bacterium protein content, while making fermented feed that there is the mellowness of concentration, improves the palatability and attractant of feed, increase meat
The speed of growth of the feed intake of ox.
The second, preferably using complex enzyme formulation I and complex enzyme formulation II respectively to feedstuff and proferment in the present invention
Material carries out fermentation process so that beneficiating ingredient is abundant in fermented feed, rich in lactic acid bacteria metabolite, metabolism of yeasts product,
Oligopeptides, organic acid, reduced sugar, aromatic compound, unknown growth factor etc. can adjust beef cattle cud Bacterial community, promote meat
Ox growth, shortens the beef ox fattening phase, and contains a large amount of yeast cell walls and oligosaccharide in fermented feed, can adsorb common
Mycotoxin promotes immunity of organisms.
Third, method of the invention prepare the dedicated hair of beef cattle by mixing lactic acid bacteria metabolite and Yeast culture
Ferment feed, preparation method is simple, has general applicability, remarkable in economical benefits, and the composition of raw materials of beef cattle fermenting feed
More rationally, make finished product moisture content lower than 12%, it is convenient to mix with fine fodder, convenient for storage, and the meat of beef cattle can be improved,
Promote beef cattle index.
Detailed description of the invention
Fig. 1 is the excrement sample of beef cattle before not feeding fermented feed prepared by the present invention；
Fig. 2 be feed the embodiment of the present invention 1 prepare fermented feed 15 days after beef cattle excrement sample.
Specific embodiment
Below in conjunction with drawings and examples, invention is further described in detail.
Embodiment
Embodiment 1-3 neutral and alkali protease is selected from the alkali that the article No. that Jiang great Biotechnology Co., Ltd in Zhengzhou sells is 215
Property protease, neutral proteinase be selected from Zhengzhou Huambo chemical products Co., Ltd sell article No. be 0691 neutral proteinase,
Acid protease is selected from acid protease, the carbohydrase that the enzyme activity that Shandong Anke Bioengineering Co., Ltd. sells is 20,000 u/g
Selected from Zhengzhou Huambo chemical products Co., Ltd sell article No. be 0358 carbohydrase, alpha-amylase be selected from Weifang Audi you into
The alpha-amylase of export trade Co., Ltd sale, lactobacillus plantarum are selected from what sub- core (Nanjing) Biotechnology Co., Ltd sold
The lactobacillus plantarum of model LP-28, S. cervisiae are selected from the article No. that hundred Yi Bao Biotechnology Co., Ltd of Zhengzhou sells and are
The S. cervisiae of BYB072.
Embodiment 1: a kind of beef cattle fermenting feed, the lactic acid metabolism product and Yeast Cultivation for being 6:4 including mass ratio
The raw material proportioning of object, Lactic Acid Bacteria Metabolites and yeast culture is as shown in table 1, the preparation method of the beef cattle fermenting feed,
The following steps are included:
It prepares lactic acid bacteria metabolite: 0.008kg lactobacillus plantarum bacterium powder, 1kg complex enzyme system being added into 950kg feedstuff
Agent I and 450kg water, after mixing, the anaerobic fermentation 60h at 38 DEG C are after fermentation, dry using aeration-drying mode
Temperature is 80 DEG C, controls moisture content of finished products≤12%, is crushed, and lactic acid bacteria metabolite is made；
Wherein feedstuff is mixed by following components in percentage by weight: 35% corn, 5% wheat bran, 6% dregs of beans, 12%
Cotton dregs, 11% maize peel, 1% alfalfa meal, 14% corn germ cake and 16% vinasse albumen feed；Complex enzyme formulation I is by quality
Than alkali protease, neutral proteinase and the acid protease composition for 7:2:1, wherein alkali protease 700g, neutral protein
The enzyme activity of enzyme 200g, acid protease 100g, alkali protease are 200000u/g, and the enzyme activity of neutral proteinase is
100000u/g, the enzyme activity of acid protease are 20000u/g；
It prepares yeast culture: 14.25kg complex enzyme formulation II and 450kg water being added into 950kg fermentation raw material, at 65 DEG C
8h is digested to digesting completely, 4.75kg saccharomyces cerevisiae powder and 95kg water is added, after mixing, the aerobic fermentation 48h at 30 DEG C,
In fermentation process when material temperature is more than 35 DEG C, stirring cooling, when temperature of charge is not up to 35 DEG C, every 10h stirring is primary, fermentation
After, aeration-drying mode, drying temperature is 60 DEG C, controls moisture content of finished products≤12%, is crushed, yeast culture obtained；
Wherein fermentation raw material is made by corn and wheat bran by mixing, high-temperature sterilization, cooling, and corn accounts for fermentation raw material gross weight
75%, wheat bran accounts for the 25% of fermentation raw material gross weight, and high sterilising temp is 135 DEG C, and sterilization time is 8 seconds, complex enzyme formulation II by
Mass ratio be 2:1 carbohydrase and alpha-amylase composition, wherein carbohydrase be 9.5kg, alpha-amylase 4.75kg, carbohydrase
Enzyme activity is 100,000 u/g, and the enzyme activity of alpha-amylase is 5000u/g；The bacterium amount of S. cervisiae powder is 20,000,000,000/g；
It prepares fermented feed: lactic acid bacteria metabolite and yeast culture being uniformly mixed according to the mass ratio of 6:4, obtain hair
Ferment feed.
The fermented feed be suitable for wean after each stage beef cattle, adding proportion be fattening 50g/ early period head/day, fatten
Mid-term 80g/ head/day fattens later period 120g/ head/day.
The raw material proportioning of lactic acid bacteria metabolite and yeast culture in 1 embodiment 1-3 of table
Embodiment 2: a kind of beef cattle fermenting feed, the lactic acid metabolism product and Yeast Cultivation for being 6.5:3.5 including mass ratio
The raw material proportioning of object, Lactic Acid Bacteria Metabolites and yeast culture is as shown in table 1, the preparation method of the beef cattle fermenting feed,
The following steps are included:
It prepares lactic acid bacteria metabolite: 0.009kg lactobacillus plantarum bacterium powder, 1.2kg complex enzyme being added into 980kg feedstuff
Preparation I and 480kg water, after mixing, the anaerobic fermentation 66h at 39 DEG C using aeration-drying mode, are done after fermentation
Dry temperature is 85 DEG C, controls moisture content of finished products≤12%, is crushed, and lactic acid bacteria metabolite is made；
Wherein feedstuff is mixed by following components in percentage by weight: 36% corn, 3% wheat bran, 5% dregs of beans, 10%
Cotton dregs, 12% maize peel, 2% alfalfa meal, 15% corn germ cake and 17% vinasse albumen feed；Complex enzyme formulation I is by quality
Than alkali protease, neutral proteinase and the acid protease composition for 7:2:1, wherein alkali protease 840g, neutral protein
The enzyme activity of enzyme 240g, acid protease 120g, alkali protease are 200000u/g, and the enzyme activity of neutral proteinase is
100000u/g, the enzyme activity of acid protease are 20000u/g；
It prepares yeast culture: 17.5kg complex enzyme formulation II and 480kg water being added into 980kg fermentation raw material, at 68 DEG C
9h is digested to digesting completely, 6.5kg saccharomyces cerevisiae powder and 90kg water is added, after mixing, the aerobic fermentation 54h at 31 DEG C,
In fermentation process when material temperature is more than 35 DEG C, stirring cooling, when temperature of charge is not up to 35 DEG C, every 11h stirring is primary, fermentation
After, aeration-drying mode, drying temperature is 63 DEG C, controls moisture content of finished products≤12%, is crushed, yeast culture obtained；
Wherein fermentation raw material is made by corn and wheat bran by mixing, high-temperature sterilization, cooling, and corn accounts for fermentation raw material gross weight
78%, wheat bran accounts for the 22% of fermentation raw material gross weight, and high sterilising temp is 138 DEG C, and sterilization time is 6 seconds, complex enzyme formulation II by
Mass ratio be 2:1 carbohydrase and alpha-amylase composition, wherein carbohydrase be 11.6kg, alpha-amylase 5.9kg, carbohydrase
Enzyme activity is 100,000 u/g, and the enzyme activity of alpha-amylase is 5000u/g；The bacterium amount of S. cervisiae powder is 20,000,000,000/g；
It prepares fermented feed: lactic acid bacteria metabolite and yeast culture being uniformly mixed according to the mass ratio of 6.5:3.5, that is, made
Obtain fermented feed.
The fermented feed be suitable for wean after each stage beef cattle, adding proportion be fattening 65g/ early period head/day, fatten
Mid-term 100g/ head/day fattens later period 135g/ head/day.
Embodiment 3: a kind of beef cattle fermenting feed, the lactic acid metabolism product and Yeast Cultivation for being 7:3 including mass ratio
The raw material proportioning of object, Lactic Acid Bacteria Metabolites and yeast culture is as shown in table 1, the preparation method of the beef cattle fermenting feed,
The following steps are included:
It prepares lactic acid bacteria metabolite: 0.01kg lactobacillus plantarum bacterium powder, 1.5kg complex enzyme being added into 1000kg feedstuff
Preparation I and 500kg water, after mixing, the anaerobic fermentation 72h at 40 DEG C using aeration-drying mode, are done after fermentation
Dry temperature is 95 DEG C, controls moisture content of finished products≤12%, is crushed, and lactic acid bacteria metabolite is made；
Wherein feedstuff is mixed by following components in percentage by weight: 40% corn, 2% wheat bran, 6% dregs of beans, 12%
Cotton dregs, 11% maize peel, 1% alfalfa meal, 12% corn germ cake and 16% vinasse albumen feed；Complex enzyme formulation I is by quality
Than alkali protease, neutral proteinase and the acid protease composition for 7:2:1, wherein alkali protease 1050g, neutral protein
The enzyme activity of enzyme 300g, acid protease 150g, alkali protease are 200000u/g, and the enzyme activity of neutral proteinase is
100000u/g, the enzyme activity of acid protease are 20000u/g；
It prepares yeast culture: 20kg complex enzyme formulation II and 500kg water, the enzyme at 70 DEG C being added into 1000kg fermentation raw material
10h is solved to digesting completely, 10kg saccharomyces cerevisiae powder and 100kg water is added, after mixing, the aerobic fermentation 60h at 32 DEG C, hair
During ferment when material temperature is more than 35 DEG C, stirring cooling, when temperature of charge is not up to 35 DEG C, every 12h stirring is primary, fermentation knot
Shu Hou, aeration-drying mode, drying temperature are 65 DEG C, control moisture content of finished products≤12%, crush, yeast culture obtained；
Wherein fermentation raw material is made by corn and wheat bran by mixing, high-temperature sterilization, cooling, and corn accounts for fermentation raw material gross weight
80%, wheat bran accounts for the 20% of fermentation raw material gross weight, and high sterilising temp is 140 DEG C, and sterilization time is 4 seconds, complex enzyme formulation II by
Mass ratio be 2:1 carbohydrase and alpha-amylase composition, wherein carbohydrase be 13.4kg, alpha-amylase 6.7kg, carbohydrase
Enzyme activity is 100,000 u/g, and the enzyme activity of alpha-amylase is 5000u/g；The bacterium amount of S. cervisiae powder is 20,000,000,000/g；
It prepares fermented feed: lactic acid bacteria metabolite and yeast culture being uniformly mixed according to the mass ratio of 7:3, obtain hair
Ferment feed.
The fermented feed be suitable for wean after each stage beef cattle, adding proportion be fattening 80g/ early period head/day, fatten
Mid-term 120g/ head/day fattens later period 150g/ head/day.
Comparative example
Comparative example 1: a kind of beef cattle fermenting feed, the difference from embodiment 1 is that, lactic acid bacteria metabolite and Yeast Cultivation
The mass ratio of object is 5:3.
Comparative example 2: a kind of beef cattle fermenting feed, the difference from embodiment 1 is that, lactic acid bacteria metabolite and yeast
The mass ratio of culture is 8:3.
Comparative example 3: a kind of beef cattle fermenting feed, the difference from embodiment 1 is that, lactic acid bacteria metabolite and yeast
The mass ratio of culture is 7:2.
Comparative example 4: a kind of beef cattle fermenting feed, the difference from embodiment 1 is that, lactic acid bacteria metabolite and yeast
The mass ratio of culture is 7:5.
Comparative example 5: with the embodiment system in the Chinese invention patent application file application No. is CN201811125035.2
Standby fermented feed is as control, a kind of preparation method of beef cattle fully mixed diet fermented feed, comprising the following steps:
(1) impurity removing is removed into stevia rebaudiana dregs sieving；By 5.5% vitamin premix, 22% trace element premix agent, 1.0% second
Oxygen quinoline and surplus are that powdered rice hulls form compound premix；
(2) 57 parts of stevia rebaudiana dregs in (1), 1 part of compound premix and 22 parts of corn, 7 parts of rice bran, 8.6 parts of cottonseed protein, phosphoric acid are taken
0.9 part of hydrogen calcium, 0.6 part of mountain flour, 1.0 parts of sodium bicarbonate, 0.6 part of salt, 0.8 part of leavening, 0.5 part of molasses be added in mixing machine
It is mixed, feed moisture content is adjusted in mixed process to 42%；
(3) raw material is packed into after mixing in polyethylene film fermentation bag, is stored at room temperature after sack sealing, after fermented 21 days
Can break a seal feeding.
Performance detection test
One, in fermented feed nutrient composition content test
Beef cattle fermenting feed is prepared according to the method in embodiment 1-3, and according to seeking in following standard detection fermented feed
The content formed point, testing result are recorded in table 2:
Moisture: it is tested according to GB/T6435-2006 " measurement of moisture and other volatile matter contents in feed "；Thick egg
It is white: to be measured according to GB/T6432-1994 " crude protein determining method in feed "；Crude fibre: according to GB/T6434-2006
" coarse-fibred assay filtration method in feed " is measured；Coarse ash: according to GB/T6438-2007 " coarse ash in feed
Measurement " be measured；Calcium: it is measured according to GB/T6436-2002 " calcium catalyst in feed "；Total phosphorus: according to GB/
T6437-2002 " total phosphorus yield spectrophotometry in feed " is measured；The molten albumen of acid: according to Q/YS001-2016 " feed
Fermenting raw materials dregs of beans " in appendix A tested；Beta glucan: it is surveyed according to QB/T4572-2013 " yeast beta-dextran "
Examination.
The nutrient component determining of the beef cattle fermenting feed of 2 embodiment 1-3 of table preparation
Each nutrient composition content is equal in the beef cattle fermenting feed prepared it can be seen from data in table 2 by embodiment 1-3
Weighing apparatus, index stabilization, and moisture content are lower than 12%, crude protein >=15%, crude fibre≤12%, coarse ash≤7%, and calcium 0.2~
1.0%, total phosphorus >=0.5%, sodium chloride 0.3~1.3%, molten albumen (accounting for crude protein) >=30%, pH≤4.5 of acid, antibacterial peptide effect
Valence >=1000U/g, mannosan >=1%, beta glucan >=2%, zymosan >=5%, it is convenient to mix with fine fodder, and convenient for mentioning
It rises beef cattle growth indexes and improves meat, while the pH of fermented feed maintains 4.11-4.32, can inhibit pathogenic thin in enteron aisle
The breeding and growth of bacterium and pathogen.
Two, test of the fermented feed to the beef cattle speed of growth
Beef cattle fermenting feed is prepared according to the method in embodiment 1-3 and comparative example 1-5, and detection hair in accordance with the following methods
Influence of the ferment feed to the beef cattle speed of growth:
1, experimental animal: beef cattle 240 to be in a good state of health using body condition, weight, weight is at 230 kilograms or so；
2, test method: 240 beef cattles are randomly divided into 8 groups, every group 30, measure the first initial body of every group of 30 beef cattles respectively
Weight, every group is averaged, and 8 groups of beef cattles are freely eaten respectively by fermented feed 3 times of embodiment 1-3 and comparative example 1-5 preparation, remembers
Feed consumption is recorded to measure the weight of every group of 30 beef cattles after feeding 100 days, and be averaged, be denoted as opisthosoma weight, and calculate
Feedstuff-meat ratio will deliver the beef cattle butchered for sale, remove head, hoof, blood, fur, internal organ, remaining calculates ketoboidies weight as ketoboidies weight
The ratio between amount and live weight, obtain dressing percentage, testing result is as shown in table 3.
The growth rate and feedstuff-meat ratio of the test beef cattle of table 3
The fermented feed that in edible embodiment 1-3 it can be seen from data in table 3 prepared by method, beef cattle increase in 100 days
Speed is fast, and every daily gain reaches 1.59kg or more, and feedstuff-meat ratio is lower than 6.21, and dressing percentage reaches 57.83% or more, illustrates this hair
The beef cattle fermenting feed of bright embodiment 1-3 preparation has apparent growth promoting function to beef cattle, can shorten the fattening of beef cattle
In the period, efficiency of feed utilization is high, and beef cattle can fully absorb the nutriment in feed, and completely, breeding efficiency is high for nutrition conversion.
Because the weight ratio of lactic acid bacteria metabolite and yeast culture is respectively 5:3 and 8:3 in comparative example 1-2, eat pair
The beef cattle of fermented feed prepared by ratio 1 and comparative example 2 is compared with embodiment 1-3, and the speed of growth is slightly slow, and daily gain is small, expects meat
Become larger, dressing percentage becomes smaller, and illustrates make meat when the content of Lactic Acid Bacteria Metabolites is more or very few relative to yeast culture
The speed of growth of ox is slack-off, and nutrient absorption is few, reduces the utilization rate and breeding efficiency of feed.
Because the mass ratio of lactic acid bacteria metabolite and yeast culture is respectively 7:2 and 7:5 in comparative example 3-4, eat by
Fermented feed beef cattle daily gain prepared by comparative example 3 and comparative example 4 is small, and feedstuff-meat ratio significantly increases, and illustrates yeast culture content
When more or less, the utilization rate of feed can be reduced, beef cattle is poor to the absorptivity of nutriment, and beef cattle growth is more slow.
Comparative example 5 is the feed of prior art preparation, and after beef cattle is edible, daily gain is only 1.25kg, feedstuff-meat ratio 6.59,
Dressing percentage is 57.24%, and compared with embodiment 1-3, efficiency of feed utilization and promotion beef cattle growth are obviously deteriorated, and illustrates this hair
The fermented feed of bright preparation plays the role of excellent promotion beef cattle growth, can be improved the utilization rate of feed, shortens educating for beef cattle
The fertile period keeps breeding efficiency higher.
Three, influence of the fermented feed to beef cattle digestibility
Beef cattle fermenting feed is prepared according to method in embodiment 1-3 and comparative example 1-4, chooses body condition, weight health status
Good beef cattle 240, the cattle feed that feeding is 510 by the article No. that Liaoning Boer Laite Farming-Grazing Industrial Co., Ltd. sells,
The weight that residue in 240 Feces of Beef Cattle is detected using classifying screen, is averaged, and is denoted as excrement residual quantity before feeding, specifically
Data are as shown in table 4, and it is as shown in Figure 1 to feed preceding excrement screening；
Beef cattle is randomly divided into 8 groups, every group 30,8 groups of beef cattles eat the hair prepared by embodiment 1-3 and comparative example 1-5 respectively
Ferment feed, it is additional to add beef cattle fermenting feed 100g/ head/day under conditions of Basic drawing, it feeding cycle 15 days, takes
With the Feces of Beef Cattle of detection equivalent before feeding, Feces of Beef Cattle residue weight in every level-one sieve after being raised with classifying screen detection,
Excrement of the excrement of the beef cattle of fermented feed prepared by embodiment 1 after feeding is wherein eaten to screen as shown in Fig. 2, eating each reality
The Feces of Beef Cattle residue testing result for applying the fermented feed of example and the preparation of each comparative example is as shown in table 5.
The excrement residue detection of beef cattle before the fermented feed that table 4 feeds embodiment 1-3 and prepared by comparative example -5
| Project | Upper sieve | Middle layer sieve | Lower screen | Summation |
| Weight in wet base/g | 120 | 346 | 87 | 553 |
| Percentage/% | 21.7 | 62.6 | 15.7 | 100 |
Table 5 feeds the residues detection of Feces of Beef Cattle after 15d fermented feed
It can be seen from data in table 4 when not feeding the feed of embodiment 1-3 and comparative example 1-4 preparation, upper sieve ratio is
21.7%, middle layer sieve ratio example is 62.6%, and lower screen ratio is 15.7%, and middle layer sieve and upper sieve excrement ratio are obviously higher,
Significantly more than normal value 50%, and in Fig. 1 it can be seen from upper sieve residue it is more, it is apparent that indigested cotton
Seed and corn etc. illustrate that the function of rumen of beef cattle is impaired, and fiber cannot be digested and be decomposed well in cud, feed
Digestibility is lower.
After feeding feed 15 days prepared by embodiment 1-3 and comparative example 1-4, implement it can be seen from data in table 5
The excrement of the beef cattle fed in example 1 is 163g in excrement gross weight at the middle and upper levels, accounts for the 43.8% of excrement gross weight, less than 50%, and by
As can be seen that having not seen indigested cottonseed and corn etc. in upper sieve in Fig. 2, illustrate that the cud health status of beef cattle is changed
Kind, the digestion of fermented feed and absorptivity are improved.
The excrement of the beef cattle fed in same embodiment 2-3, is respectively 159g and 166g in excrement gross weight at the middle and upper levels, accounts for excrement
Just the 42.2% and 43.5% of gross weight, respectively less than 50% illustrate that the beef cattle fermenting feed of embodiment 2-3 preparation can improve
The rumen conditions of beef cattle improve the digestibility and absorptivity of fermented feed.
Feed made from comparative example 1-5, after feeding 15 days, Feces of Beef Cattle is in large percentage at the middle and upper levels, in the ratio of bottom
Example is smaller, illustrates that beef cattle reduces the absorptivity and digestibility of the comparative example 1-4 fermented feed prepared.
This specific embodiment is only explanation of the invention, is not limitation of the present invention, those skilled in the art
Member can according to need the modification that not creative contribution is made to the present embodiment after reading this specification, but as long as at this
All by the protection of Patent Law in the scope of the claims of invention.
Claims (10)
1. a kind of beef cattle fermenting feed, which is characterized in that the lactic acid bacteria metabolite and ferment for being 6-7:3-4 including mass ratio
Female culture；
The Lactic Acid Bacteria Metabolites include the raw material of following parts by weight: 950-1000 parts of feedstuffs, 0.008-0.01 portions of plants
I and 450-500 parts of lactobacillus bacterium powder, 1-1.5 parts of complex enzyme formulations water；
The yeast culture includes the raw material of following parts by weight: 950-1000 parts of fermentation raw materials, 14.25-20 parts of complex enzyme systems
II, 4.75-10 parts of S. cervisiae powder of agent, 545-600 parts of water.
2. beef cattle fermenting feed according to claim 1, which is characterized in that the feedstuff includes following weight
The raw material of percentage: corn 35-40%, wheat bran 2-5%, dregs of beans 4-6%, cotton dregs 10-12%, maize peel 11-15%, alfalfa meal 1-2%,
Corn germ cake 12-15%, vinasse albumen feed 16-20%.
3. beef cattle fermenting feed according to claim 1, which is characterized in that the fermentation raw material includes following weight
The raw material of percentage: corn 75-80%, wheat bran 20-25%.
4. beef cattle fermenting feed according to claim 1, which is characterized in that the complex enzyme formulation I is by mass ratio
It being formed for the alkali protease, neutral proteinase and acid protease of 7:2:1, the enzyme activity of alkali protease is 200000u/g,
The enzyme activity of neutral proteinase is 100000u/g, and the enzyme activity of acid protease is 20000u/g.
5. beef cattle fermenting feed according to claim 1, which is characterized in that the complex enzyme formulation II is by mass ratio
It is formed for the carbohydrase and alpha-amylase of 2:1, the enzyme activity of carbohydrase is 100,000 u/g, and the enzyme activity of alpha-amylase is 5000u/
g。
6. beef cattle fermenting feed according to claim 1, which is characterized in that the bacterium amount of the lactobacillus plantarum bacterium powder
For 400,000,000,000/g, the bacterium amount of the S. cervisiae powder is 20,000,000,000/g.
7. a kind of preparation method of beef cattle fermenting feed according to claim 1-6, which is characterized in that packet
Include following steps:
It prepares lactic acid bacteria metabolite: lactobacillus plantarum bacterium powder, complex enzyme formulation I and water being added into feedstuff, mixing is equal
After even, the anaerobic fermentation 60-72h at 38-40 DEG C is after fermentation, dry, controls moisture content of finished products≤12%, crushes, cream is made
Sour bacterium metabolite；
Wherein feedstuff is by corn, wheat bran, dregs of beans, cotton dregs, maize peel, alfalfa meal, corn germ cake and vinasse albumen feed
It is mixed according to a certain percentage；
It prepares yeast culture: complex enzyme formulation II and water being added into fermentation raw material, 8-10h is digested at 65-70 DEG C to enzyme
Completely, saccharomyces cerevisiae powder and remaining water, after mixing, aerobic fermentation 48-60h, fermentation ends at 30-32 DEG C is added in solution
Afterwards, dry, moisture content of finished products≤12% is controlled, is crushed, yeast culture obtained；
Wherein material issuance is mixed according to a certain percentage by corn and wheat bran, high-temperature sterilization, cooling is made；
Fermented feed: lactic acid bacteria metabolite and yeast culture are proportionally uniformly mixed, fermented feed is obtained.
8. the preparation method of beef cattle fermenting feed according to claim 7, which is characterized in that preparation lactic acid bacteria metabolism
When product, using aeration-drying mode, drying temperature is 80-95 DEG C, when preparing yeast culture, using aeration-drying mode,
Drying temperature is 60-65 DEG C.
9. the preparation method of beef cattle fermenting feed according to claim 7, which is characterized in that prepare yeast culture
When, in fermentation process when material temperature is more than 35 DEG C, stirring cooling, when temperature of charge is not up to 35 DEG C, every 10-12h stirring one
It is secondary.
10. a kind of application method of beef cattle fermenting feed according to claim 1-6, which is characterized in that
Suitable for each stage beef cattle after weaning, adding proportion is to fatten 50~80g/ early period head/day, fattening 80~120g/ of mid-term head/
It, fattens 120~150g/ of later period head/day.
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| CN111587954A (en) * | 2020-06-17 | 2020-08-28 | 湖南华佑生物科技有限公司 | Feed particle for improving beef quality and preparation method thereof |
| CN111700162A (en) * | 2020-07-22 | 2020-09-25 | 四川力信生物技术有限公司 | Feed for solving problem of indigestibility of intestinal tracts of distillers' grains fed cows and preparation method thereof |
| CN112244167A (en) * | 2020-11-10 | 2021-01-22 | 彭阳县荣发农牧有限责任公司 | Preparation method of fermented compound feed for pregnant cows |
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| CN109198162A (en) * | 2018-10-29 | 2019-01-15 | 江南大学 | A kind of method that bacterium enzyme cooperative fermentation prepares dregs of beans |
| CN109258944A (en) * | 2018-11-21 | 2019-01-25 | 贵州金凯禾科技综合开发有限公司 | A kind of vinasse fermentation feed that beef cattle is edible |
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Application publication date: 20190726 |