CN109007333A - It is a kind of to prepare ruminant ferment bacterium fuel and its preparation process using winter jujube inferior fruit - Google Patents
It is a kind of to prepare ruminant ferment bacterium fuel and its preparation process using winter jujube inferior fruit Download PDFInfo
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- CN109007333A CN109007333A CN201810819908.3A CN201810819908A CN109007333A CN 109007333 A CN109007333 A CN 109007333A CN 201810819908 A CN201810819908 A CN 201810819908A CN 109007333 A CN109007333 A CN 109007333A
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- winter jujube
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
- A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
Ruminant ferment bacterium fuel and its preparation process are prepared using winter jujube inferior fruit the present invention relates to a kind of, belongs to the additive for microbe feedstuff in biological feedstuff field and its fabricating technology field.Including mixture, microbiological feed leavening, additive, clear water, above-mentioned four weight ratio is 100:(0.0003-0.0005): 0.001:(60-65)；Mixture includes winter jujube 70%, brewex's grains 20%, corn flour 10%, and additive is brown sugar water, and brown sugar water concentration is 10%.The present invention turns waste into wealth winter jujube inferior fruit, be prepared into can with long term storage, feeding value is high, have healthcare function, containing high-activity probiotics, palatability is good, production cost is low.
Description
Technical field
Ruminant ferment bacterium fuel and its preparation process are prepared using winter jujube inferior fruit the present invention relates to a kind of, is belonged to
Additive for microbe feedstuff and its fabricating technology field in biological feedstuff field.
Background technique
It antibiotic is excessively used is endangered caused by human body and be well-known thing, but some raisers are in order to mention
High yield avoid livestock catch and it is dead, so usage amount is often still continuously increased in the breeding process, in this height
It selects under pressure, drug-fast bacteria is largely formed.And antibiotic food is eaten for a long time in the mankind, Endophytic bacteria can also generated anti-
Property.
In recent years, as what the fast development of biotechnology, intestinal microecology were studied deepens continuously and approve, fermented feed
In animal husbandry using more and more extensive.Fermented feed is using mixed feed as fermentation substrate, and selection is inoculated with specific micro- life
Object, the conditions such as manual control temperature, moisture, oxygen demand, what is produced by microorganism self-reproduction and metabolism is rich in high activity
The feed of probiotics and its metabolite.Compared with conventional feed, fermented feed low, nutritive value with anti-nutritional factors content
High, the advantages that small molecular protein and peptides content are high.How it is existing that fermented feed technology needs the main problem solved
It is few with or without using antibiotic in feed under the conditions of feeding environment.Here there is two layers of meaning: first is that without using antibiotic
In the case where ensure the general level of the health of animal；Second is that this technology have actual use value, can be animal husbandry bring through
Ji benefit, while also generating good social benefit.
An important factor for scarcity of feedstuff and its cost problem are always restriction aquaculture development, and get in recent years
It drills stronger.Therefore, exploitation novel fodder resource is extremely urgent as early as possible.Conventional feed mostly based on the grains such as corn, is striven with the mankind
Grain and expensive；Although and the agricultural byproducts such as various poor slags, stalk are cheap, contain cellulose, lignin and pancreas egg
The anti-nutritional factors such as white enzyme inhibitor, animal is difficult to digest and assimilate, and palatability is poor, therefore feeding value is not high.By micro-
Biofermentation means can then improve product quality and palatability come the agricultural byproduct that ferments.These agricultural and sideline products are sent out through probiotics
It is a variety of that various enzymes, vitamin, amino acid, small peptide, antibacterial substance, the Immune-enhancing effect factor, somatomedin etc. can be transformed into after ferment
Nutriment, these substances can improve feed palatability, improve feed intake, and be beneficial to cultivated animals intestinal health, promote
Growth of animal.Content of toxins can also be reduced simultaneously, improved pollution of the feed to environment, finally provided for the mankind all natural nuisanceless
The high feed product of nutritive value.But conventional feed fermentation needs to be related to a variety of operations such as raw material disinfection and finished product drying, no
Only complex procedures, and energy consumption is very big, therefore is badly in need of developing a kind of simple fermentation technique of reduction energy consumption process.
In addition feed storage is also the serious problem put in face of us.Especially rainwater is more, and humidity is larger in air,
In the case where summer high temperature, once storage feed divulge information it is unsmooth in the case where, when pH value is suitable for, it is easy to make moist
It is mouldy.Under mouldy not serious situation, be not easy to find, but feeding such feed, cultivated animals but probably because
Excessively eat certain mycotoxins and mortality.
With poultry industry scale, intensive development, the pernicious gas generated in breeding process has been environmental pollution
One important sources.Pernicious gas reaches the resistivity and production performance that a certain concentration can reduce animal to disease in animal house,
Would generally using ventilation, place adsorbent or sprinkling chemical deodorizing agent and be added in feed the methods of additive come
Reduce pernicious gas content in animal house.The working strength of raiser, and the chemical deodorizing agent sheet sprayed not only are increased in this way
Body is also harmful to human body.So being badly in need of developing a kind of simple and convenient and environmentally friendly breeding way.Feeding containing profitable probliotics
Material is proliferated, the growth for the saprophytic bacterias such as Escherichia coli that can reject by after livestock edible in animal intestinal tract, changes metabolism side
Formula, to reduce the generation of pernicious gas.Other probiotics can be discharged with excrement, and continuation plays decomposition in vitro.
Winter jujube (Ziziphus jujuba Mill.cv Dongzao) belongs to Rhamnaceae zizyphus, also known as tricolour amaranth, apple jujube
Deng, be mainly distributed on Hebei, Shandong have a common boundary Bay areas, influenced by its unique geographical environment and humanistic environment, the winter
Jujube pulp delicacy succulence, mouthfeel sweet tea are crisp, full of nutrition.Winter jujube fruit is big, subcircular, 2.9~3.2cm of longitudinal and transverse demeter.Contain in jujube fruit
A large amount of carbohydrate (nearly 250g/kg fresh fruit) contains more jujube oligosaccharide, jujube based on sucrose, fructose, glucose
Oligosaccharide is good prebiotics, can promote animal intestinal tract growth of probiotics.Also containing asparatate, Soviet Union's ammonia in winter jujube
19 kinds of amino acid needed by human such as acid, serine, total content 0.985mg/100g；Containing protein 1.65%；Dietary fiber
2.3%；Niacin 0.87mg/100g；1.1 μ g/g of carrotene；0.1 μ g/g of vitamin B1；2.2 μ g/g of vitamin B2；Vitamin
The content of C is especially abundant, reaches 3.52mg/g, is 70 times of apple Vitamin C content, and 140 times of pears, 20 times of golden jujube,
There is the good reputation of " vitamin pill living ".In addition, also to contain more VitAVitE, potassium, sodium, iron, copper etc. more for winter-jujube fruit
Kind of microelement has and keeps that capillary is unimpeded, prevents the increased function of vascular wall brittleness, for hypertension, Atherosclerosis
It is effective in cure to change illness, there is the effect of anti-cancer.Flavonoid substances are that a kind of important functional component, general flavone content are reachable in winter jujube
2.6mg/g date powder, winter jujube flavonoid component is complicated, and rutin content is higher in jujube fruit, flavones object all rich in fructus corni, jujube core
Matter has antioxidation in vitro and anti-tumor activity.Contain the substances such as higher cyclic adenosine monophosphate in winter jujube.Camp Content
Up to 239.30 μ g/g date powders.Cyclic adenosine monophosphate is a kind of important physiological activator, a variety of physiological activities in participant's body,
Adjustable function of immune system has enhancing myocardial contractive power, protection coronary artery, reduces cholesterol, cancer cell is inhibited to increase
The effect grown.Therefore, winter jujube is a kind of extraordinary dual-purpose of drug and food food.
In recent years, with the construction of Agricultural Park, winter jujube industry is developed rapidly.Such as Zhanhua of several major production areas,
The every annual planting area in Huanghua area is all up to 20,000 hm2, total yield is more than 3.25 hundred million kg.Winter jujube belongs to seasonal fruit, sells too late
When be easy to rot.The fruit that will cause nearly half due to weather or management measure are improper etc. every year is inferior fruit (containing falling
Ground fruit, decayed fruit).Orchard workers' majority is that it is ground into date powder directly to do feed use.But due to cellulose, hemicellulose, wood
Quality and non-digestible property albumen equal size are higher, and feeding value is not very high.Winter jujube is after everfermentation, in the side of microorganism
It helping down, the nutriment of some bad digestion can be converted to the lesser substance of molecular weight, thus be easy by animal intestinal absorption,
Increase substantially feeding value.This technology not only can solve the storage problem of rotten jujube, and can bring can once promoting
The economic benefit of sight.The fermentation country of non-edible Chinese date is reported and few, and the fermentation of winter jujube recycling has not been reported.It utilizes
Unedible jujube substitutes corn, produces functional feed, environment-protecting feed, while reducing feed cost raising immunity of organisms,
Nitrogen discharge and phosphorus discharge is reduced, promotes the safety of feed, which does not break through so far.
Summary of the invention
It is an object of the invention to solve the shortcomings of the prior art, provide it is a kind of by winter jujube inferior fruit change give up into
Treasured, be prepared into can with long term storage, feeding value is high, have healthcare function, containing high-activity probiotics, palatability is good, production
The preparation method of nonreactive ferment bacterium fuel at low cost.This method using whole grain winter jujube inferior fruit as primary raw material, compounding brewex's grains,
Corn flour etc. carries out solid-state anaerobism raw material fermentation under external source microbial action, the full price with the ferment bacterium fuel with synthesis
When feed raises the ruminants such as cattle and sheep, it can be improved by the production performance and immunocompetence of raising animal, animal meat can be made
It is delicious, ammonia, concentration of hydrogen sulfide in animal house are reduced, there is apparent economic benefit and social benefit.
A kind of ruminant ferment bacterium fuel prepared using winter jujube inferior fruit, is characterized in that including following original
Material:
Mixture, microbiological feed leavening, additive, clear water, above-mentioned four weight ratio are 100:(0.0003-
0.0005): 0.001:(60-65)；
The mixture includes winter jujube 70%, brewex's grains 20%, corn flour 10%；
The microbiological feed leavening includes profitable strain and complex enzyme formulation, and microbiological feed leavening is purchased from benefit
Add prebiotic object Engineering Co., Ltd, name of product is increasingly prebiotic object feed fermentation agent；
The profitable strain is lactic acid bacteria, saccharomycete, bacillus, Bifidobacterium, photosynthetic bacteria, appointing in nitrobacteria
The one or more of mixing of meaning；
The complex enzyme formulation is digestive ferment, protease, amylase, cellulase, amino acid, any one in vitamin
Kind or several mixing；
Micro organism quantity is greater than 15,000,000,000 cfu/g in the microbiological feed leavening；
The additive is brown sugar water, and brown sugar water concentration is 10%；
The winter jujube is inferior jujube, including stammerer jujube, landing jujube and the jujube that slightly goes mouldy；
The winter jujube is greater than for moisture content after drying lower than 15%, 1% or more protein content, soluble sugar content
30% winter jujube；
The brewex's grains are dry slag made of preparing the drying of the insoluble matters such as the barley skin after brewer's wort in brewing, thick
Protein content is greater than 22%, and crude fat content is greater than 7%.
The present invention also provides it is a kind of using winter jujube inferior fruit preparation ruminant ferment bacterium fuel preparation method,
Be characterized in that itself the following steps are included:
1) actication of culture
Brown sugar is added in clear water and is configured to brown sugar water, brown sugar water is boiled into disinfection, when being subsequently cooled to 30 DEG C, to brown sugar
Microbiological feed leavening is added in water, seals shake culture 6-12h at 30-32 DEG C, activation bacterium solution is made；
The weight ratio of both the microbiological feed leavening, brown sugar water is (0.3-0.5): 1；
The concentration of the brown sugar water is 10%；
2) mix is prepared
Granular winter jujube inferior fruit and brewex's grains will be crushed to, mix is made after mixing；
Mass ratio between the winter jujube and brewex's grains is 7:2；
It is described to crush winter jujube inferior fruit with Double-grade disintegrating mill, grinding particle size 0.1-0.3cm；
The winter jujube inferior fruit is first dried before crushing, and is crushed again after dry, and moisture content is lower than 15% after drying；
3) corn flour is added
Corn flour is added in step 1) activation bacterium solution obtained, into activation bacterium solution, addition is boiled after cooling clear
Water is added in mix made from step 2) after mixing；
The corn flour, activation bacterium solution, the weight ratio of clear water are 10:(0.0013-0.0015): (60-65)；
It is described to be added in mix made from step 2 by the way of stirring while adding after mixing；
4) humidity is adjusted
Supplement clear water reaches the 60-65% of amount of dry matter to total amount of water, and humidity control is holding one at sticking together, firmly
The degree not being discharged is held to get fermentation material；
5) it ferments
By fermentation material anaerobic fermentation 5-10 days at 16-30 DEG C, when measuring fermentation material pH less than 4.0, and clump count reaches
When 1000000000 cfu/g, fermentation material has alcohol ester sour taste, terminates fermentation and obtains ferment bacterium fuel；
Flavones content after fermentation in ferment bacterium fuel is greater than 250mg/100g, and cycli phosphate gland acid content is greater than 20mg/
100g, ascorbic content are greater than 240mg/100g；
The specific steps of the fermentation are as follows: the fermentation material mixed is packed into the PE film with one-way exhaust breather valve and is sealed
In packaging bag, film thickness is 20-25, and charge is the 70% of packaging bag volume, is pressed and sealed, heap fermentation after packaging, one
3-8 bags of heap, summer is aeration-cooling with high-power exhaust fan, and winter stove fire or the door curtain made of cloth cover heat preservation, controls temperature at 16-30 DEG C
Lower anaerobic fermentation 5-10 days.
Product mentioned by the present invention meets as defined in People's Republic of China (PRC) agricultural industry criteria NY/T 1444-2007
Additive for microbe feedstuff technical indicator.
The present invention is a kind of to prepare ruminant ferment bacterium fuel and its preparation process using winter jujube inferior fruit, has following
Advantage:
(1) winter jujube inferior fruit is transformed into fermentation using microbiota metabolic activity by the principle that the present invention follows refuse reclamation
Feed.Reduce environmental pollution and the waste of the energy, the income of peasant can be increased substantially, therefore there is environmental protection and society's meaning
Justice；
(2) cyclic adenosine monophosphate rich in, flavone compound, vitamin C, jujube oligosaccharide etc. have and protect in winter jujube
The substance of health-care function, so that the ferment bacterium fuel prepared is with health role；
(3) microbiological feed leavening of the present invention is by lactic acid bacteria, saccharomycete, bacillus, Bifidobacterium, photosynthetic thin
The multiple beneficials such as bacterium, nitrobacteria flora and various complex enzyme formulations (digestive ferment, protease, amylase, cellulase, ammonia
Base acid, vitamin etc.) composition.Macromolecular indigestibility substance (such as cellulose, crude protein) in winter jujube can be turned rapidly
Chemical conversion livestock is easy the small-molecule substance (such as polypeptide, amino acid, glucose) being absorbed and utilized, and improves digesting for feed
Rate increases substantially feeding value.It can also continue constantly generating various decomposition enzymes in microbial metabolism simultaneously
Macromolecular substances are converted.So that the nutriment in raw material is maximally utilized, the feed after fermentation has light
Alkyd fragrance, palatability is fabulous, improves the grazing rate of livestock.Therefore production performance increases substantially；
(4) vitamin can be also generated in microbial metabolism of the present invention, antibacterial substance, the Immune-enhancing effect factor, promoted
A variety of nutriments such as growth factor accelerate livestock growth, improve high livestock immunity；There is no high temperature after the completion of feed fermentation
Processing, therefore remain a large amount of probiotics, after feed intake can balance intestinal microorganism, the saprophytic bacteria that can reject, pathogen
Growth.Therefore it is not easy with the livestock that the feed feeding of addition ferment fuel produced of the invention is crossed sick, can be reduced
The usage amount of antibiotic；
(5) microorganism of the present invention such as bacillus can decompose toxin in metabolic process, therefore can reduce crude feed
Toxin in material, so that the utilization rate of raw material increases；
(6) micropopulation of the present invention is all that beneficial flora is grown to microorganism, based on lactic acid bacteria, passes through livestock
After feeding enters in vivo, the conversion for participating in substance in animal body can be colonized, significantly reduces the content drop of propionate in excrement,
Therefore the generation of ammonia, hydrogen sulfide etc. is reduced, breeding environment is improved；
(7) of the present invention to prepare ruminant ferment bacterium fuel using winter jujube inferior fruit, it is raw material fermentation, therefore
Steam consumption is greatly reduced, to reduce production cost；
(8) fermentation installation of the present invention is the PE film sealing bags with one-way exhaust breather valve, fermentation process
In, generation gas can be vented automatically when excessive, avoid the contact for opening material with oxygen；In addition it individually packs, after opening bag
First use is complete, will not pollute, and the fermentation material in hermetic bag can be and easy to remove with long term storage；
(9) pulverizer of the present invention is bipolar pulverizer, and can both crush wet feed can also be with grinding dry.Winter jujube is big
Amount can be crushed when mature using wet feed, eliminated baking step, kept operation simpler, and can save some electrical power, pulverizer
Bottom can release after crushing material in machine in time without sieve plate, therefore powder will not be made to be in excessive temperature for a long time
Vitamins and other nutritious components are influenced under environment, pulverizer is provided with a blade more than 200, jujube core can be crushed to 0.1-
0.3cm increases the utilization rate of raw material so that the nutriment in jujube core can be also utilized.
Specific embodiment
Below with reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of ruminant ferment bacterium fuel prepared using winter jujube inferior fruit of the present embodiment, including following raw material:
Mixture, microbiological feed leavening, additive (additive is brown sugar water, brown sugar water concentration be 10%), clear water,
Above-mentioned four weight ratio is 100:(0.0003-0.0005): 0.001:(60-65)；
Wherein, mixture includes winter jujube (winter jujube is inferior jujube, for stammerer jujube or lands jujube or the jujube that slightly goes mouldy)
70%, (brewex's grains are dry slag made of preparing the drying of the insoluble matters such as the barley skin after brewer's wort in brewing to brewex's grains, thick
Protein content is greater than 22%, and crude fat content is greater than 7%) 20%, corn flour 10%；
Microbiological feed leavening includes profitable strain and complex enzyme formulation, and microbiological feed leavening is purchased from increasingly beneficial
Bioengineering Co., Ltd, name of product are increasingly prebiotic object feed fermentation agent, microbe quantity in the microbiological feed leavening
Amount is greater than 15,000,000,000 cfu/g；Wherein, profitable strain is lactic acid bacteria, saccharomycete, bacillus, Bifidobacterium, photosynthetic bacteria, nitrification
Any one or a few mixing in bacterium；Complex enzyme formulation be digestive ferment, protease, amylase, cellulase, amino acid,
Any one or a few mixing in vitamin；
Embodiment 2
The present embodiment the difference from embodiment 1 is that: winter jujube be it is dry after moisture content be lower than 15%, protein content
1% or more, soluble sugar content is greater than 30% winter jujube.
Embodiment 3
The present invention also provides a kind of preparation method using winter jujube inferior fruit preparation ruminant ferment bacterium fuel, packets
Include following steps:
1) actication of culture
Brown sugar is added in clear water and is configured to brown sugar water, brown sugar water is boiled into disinfection, when being subsequently cooled to 30 DEG C, to brown sugar
Microbiological feed leavening is added in water, seals shake culture 6-12h at 30-32 DEG C, activation bacterium solution is made, wherein microorganism
Feed fermentation agent, brown sugar water three weight ratio be (0.3-0.5): 1, the concentration of brown sugar water is 10%；
2) mix is prepared
Granular winter jujube inferior fruit and brewex's grains will be crushed to and be made mix after mixing, winter jujube and brewex's grains it
Between mass ratio be 7:2, winter jujube inferior fruit is crushed with Double-grade disintegrating mill, grinding particle size 0.1-0.3cm, winter jujube inferior fruit crush before first
It is dried, is crushed again after dry, moisture content is lower than 15% after drying；
3) corn flour is added
Corn flour is added in step 1) activation bacterium solution obtained, into activation bacterium solution, addition is boiled after cooling clear
Water mixes while stirring, is added in mix made from step 2), wherein corn flour, activation bacterium solution, the weight of clear water
Than for 10:(0.0013-0.0015): (60-65)；
4) humidity is adjusted
Supplement clear water reaches the 60-65% of amount of dry matter to total amount of water, and humidity control is holding one at sticking together, firmly
The degree not being discharged is held to get fermentation material；
5) it ferments
By fermentation material anaerobic fermentation 5-10 days at 16-30 DEG C, when measuring fermentation material pH less than 4.0, and clump count reaches
When 1000000000 cfu/g, fermentation material has alcohol ester sour taste, terminates fermentation and obtains ferment bacterium fuel, after fermentation in ferment bacterium fuel
Flavones content be greater than 250mg/100g, cycli phosphate gland acid content be greater than 20mg/100g, ascorbic content be greater than 240mg/
100g；
The specific steps of fermentation are as follows: the fermentation material mixed is packed into the PE film sealed package for having one-way exhaust breather valve
In bag, film thickness is 20-25, and charge is the 70% of packaging bag volume, is pressed and sealed, heap fermentation after packaging, a pile 3-8
Bag, summer is aeration-cooling with high-power exhaust fan, and winter stove fire or the door curtain made of cloth cover heat preservation, and control temperature is detested at 16-30 DEG C
Aerobe fermentation 5-10 days.
Product mentioned by the present invention meets as defined in People's Republic of China (PRC) agricultural industry criteria NY/T 1444-2007
Additive for microbe feedstuff technical indicator.
Embodiment 4
Feed finished cattle test
Test carries out in the Jun Di planting tree Specialty Co-operative Organization of Wudi.Experimental period is 90d.Preliminary trial period 7d.Select weight
For (236.8 ± 3.23) kg, the monthly age 7 months Simmentals 20 are randomly divided into test group and control group by homogeneous principle,
Every group of 10 oxen are further divided into 2 groups, and every group of 5 ox carries out drylot feeding, determine stake, determine slot, positioning.Every group of ox is compiled by head before testing
Number, weighing, record.Daily 6:30 carries out artificial feeding by testing crew, guarantees free diet, testing crew is in daily 17:00
It carries out the collection of surplus material and claims quality.Cleaning up excrement mode is dry cleaning up excrement, daily 7:00,14:00 cleaning up excrement.Daily 8:00,13:00,18:
The concentration of 00 measurement cowshed ammonia and hydrogen sulfide.
Control group concentrated feed proportion are as follows: maize flour 56%, dregs of beans 7%, cotton dregs 11%, wheat bran 25.5%, salt 0.3%,
Feed addictive 0.2%；
Test group and control group roughage are Silage Corn Straw.
Test group concentrated feed are as follows: the mixture of 70% control group concentrated feed and 30% ferment bacterium fuel of the present invention.
It is completed in preliminary trial period expelling parasite, the work such as immune of preventing epidemic.Smart roughage ratio is 3: 7.
Ferment bacterium fuel: the fermentation of 0.5 ‰ microbiological feeds is added in winter jujube powder 70%, brewex's grains 20%, corn flour 10%
Agent, amount of water are amount of dry matter 50% or so, and humidity control is holding one at sticking together, firmly holding the degree not being discharged.It will mix
The fermentation material got togather is fitted into the PE film sealing bags with one-way exhaust breather valve, and charge is packaging bag volume
70%, appropriate compacting sealing.Anaerobic fermentation 5 days at 25-30 DEG C, fermentation material have alcohol ester sour taste, and fermentation material pH3.8 surveys bacterium colony
Number is 1.43 × 109cfu/g, and the flavones content after detection fermentation in ferment bacterium fuel is 256mg/100g, cycli phosphate gland acid
Content is 21.3mg/100g, and ascorbic content is 244mg/100g.
Test data carries out one-way analysis of variance using 20.0 software of SPSS, carries out Multiple range test with DuncanShi method,
With P < 0.05 for significance of difference standard.
Influence of the 1 winter jujube ferment bacterium fuel of table to fat cow production performance and cowshed ammonia, concentration of hydrogen sulfide
Note: * indicates significant difference (P < 0.05)；* indicates that difference is extremely significant (P < 0.01).Similarly hereinafter；
For table the results show that the average daily gain of the ox of feeding ferment fuel of the present invention increases, feedstuff-meat ratio reduces (P <
0.05), show that the production performance of ox improves.And the ammonia, concentration of hydrogen sulfide in cowshed significantly reduce (P < 0.01), show
Cowshed environment be improved significantly.
2 beef cattle forage cost analysis of table
Note: premix provides for every kilogram of daily ration: VA 25000IU；VD 5000IU；VE 125mg；VB3 500mg；VB9
12mg；P 18g；Mg 2g；Fe 9mg；Cu 250mg；Zn 2g；Mn 1.5g；Co 15mg；I 50mg.
3 test group of table and control group Economic and Efficiency Analysis
Note: by Beef market calculation of price control group fine fodder unit price: 1.81 yuan/kg；Test group essence grain unit price: 1.81 ×
0.7+0.6 × 0.3=1.45 member/kg；Coarse fodder is Silage Corn Straw unit price: 0.08 yuan/kg.
The results show that the ox of feeding ferment fuel of the present invention, in three months, average every ox is got a profit more than control group
730.2 yuan, the net earnings of acquisition is higher than control group, and the ox for feeding ferment fuel of the present invention is with good meat quality, and sick rate is low.This
It does not also include the costs such as the reduction amount of antibiotic.
Embodiment 5
Feed Fattening Sheep test
Test carries out in the Jun Di planting tree Specialty Co-operative Organization of Wudi.Experimental period is 45d.Preliminary trial period 5d.Select weight
For (26.28 ± 4.23) kg, the Small-fat-tail sheep at 5 monthly ages 30 is randomly divided into test group and control group by homogeneous principle, and every group 3
A repetition, 5 sheep of every repetition.Drylot feeding is carried out, stake is determined, determines slot, positioning.To test sheep number, weighing, record, expelling parasite before test
And epidemic prevention, and clear up disinfection colony house.Daily 08:00 and 17:00 respectively feeds intake 1 time, is freely eaten, automatic drinking bowl drinking-water.Next day
Hopper is cleaned before feeding and weighs preceding 1 day residual feed, counts feed intake.Daily 8:00,13:00,18:00 measure sheep hurdle ammonia
With the concentration of hydrogen sulfide.
Control group concentrated feed proportion are as follows: maize flour 60%, dregs of beans 16.4%, wheat bran 12.2%, rapeseed dregs 2.4%, salt
1%, mountain flour 0.9%, sodium bicarbonate 0.5%, calcium phosphate 0.1%；
Test group and control group roughage are fresh grass.
Test group concentrated feed are as follows: the mixture of 70% control group concentrated feed and 30% ferment bacterium fuel of the present invention.
Smart roughage ratio is 3: 7.
Ferment bacterium fuel: the fermentation of 0.5 ‰ microbiological feeds is added in winter jujube powder 70%, brewex's grains 20%, corn flour 10%
Agent, amount of water are 50% or so of amount of dry matter.Humidity control is holding one at sticking together, and firmly holds the degree not being discharged.It will
The fermentation material mixed is fitted into the PE film sealing bags with one-way exhaust breather valve, and charge is packaging bag volume
70%, appropriate compacting sealing.Anaerobic fermentation 5 days at 25-30 DEG C, fermentation material have alcohol ester sour taste, and fermentation material pH3.8 surveys bacterium colony
Number is 1.43 × 109cfu/g, and the flavones content after detection fermentation in ferment bacterium fuel is 256mg/100g, cycli phosphate gland acid
Content is 21.3mg/100g, and ascorbic content is 244mg/100g.
Influence of the 4 winter jujube ferment bacterium fuel of table to fertile Productive Performance of Sheep and sheep hurdle ammonia, concentration of hydrogen sulfide
For table the results show that the average daily gain of the ox of feeding ferment fuel of the present invention increases, feedstuff-meat ratio reduces (P <
0.05), show that the production performance of sheep improves.And the ammonia, concentration of hydrogen sulfide in sheep hurdle significantly reduce (P < 0.01), show
Sheep hurdle environment be improved significantly.
5 mutton sheep forage cost analysis of table
Note: premix provides for every kilogram of daily ration: VA 25000IU；VD 5000IU；VE 125mg；VB3 500mg；VB9
12mg；P 18g；Mg 2g；Fe 9mg；Cu 250mg；Zn 2g；Mn 1.5g；Co 15mg；I 50mg.
6 test group of table and control group Economic and Efficiency Analysis
Note: it is monovalent that control group fine fodder is calculated by the mutton market price: 1.88 yuan/kg；Test group essence grain unit price: 1.88 ×
0.7+0.60 × 0.3=1.50 member/kg；Coarse fodder, which puts feeding in a suitable place to breed for green grass, can disregard cost
The results show that the ox of feeding ferment fuel of the present invention, 45d, average every sheep are got a profit 32.5 yuan more than control group,
And the mutton quality of feeding ferment fuel of the present invention is good, and sick rate is low.The net earnings of acquisition is higher than control group.This does not also include
The costs such as the reduction amount of antibiotic.
Claims (10)
1. a kind of ruminant ferment bacterium fuel prepared using winter jujube inferior fruit, it is characterised in that including following raw material:
Mixture, microbiological feed leavening, additive, clear water, above-mentioned four weight ratio are 100:(0.0003-
0.0005): 0.001:(60-65)；
The mixture includes winter jujube 70%, brewex's grains 20%, corn flour 10%；
The additive is brown sugar water, and brown sugar water concentration is 10%；
The winter jujube is inferior jujube, including stammerer jujube, landing jujube and the jujube that slightly goes mouldy.
2. a kind of ruminant ferment bacterium fuel prepared using winter jujube inferior fruit described in accordance with the claim 1, feature
It is that the microbiological feed leavening includes profitable strain and complex enzyme formulation；
The profitable strain is lactic acid bacteria, saccharomycete, bacillus, Bifidobacterium, photosynthetic bacteria, any one in nitrobacteria
Kind or several mixing；
The complex enzyme formulation be digestive ferment, protease, amylase, cellulase, amino acid, in vitamin any one or
Several mixing.
3. a kind of ruminant ferment bacterium fuel prepared using winter jujube inferior fruit described in accordance with the claim 1, feature
It is in the microbiological feed leavening that micro organism quantity is greater than 15,000,000,000 cfu/g.
4. a kind of ruminant ferment bacterium fuel prepared using winter jujube inferior fruit described in accordance with the claim 1, feature
It is that the winter jujube is greater than 30% lower than 15%, 1% or more protein content, soluble sugar content for moisture content after drying
Winter jujube.
5. a kind of ruminant ferment bacterium fuel prepared using winter jujube inferior fruit described in accordance with the claim 1, feature
It is that the brewex's grains are dry slag made of preparing the drying of the insoluble matters such as the barley skin after brewer's wort in brewing, crude protein
Matter content is greater than 22%, and crude fat content is greater than 7%.
6. it is a kind of using winter jujube inferior fruit preparation ruminant ferment bacterium fuel preparation method, it is characterised in that it include with
Lower step:
1) actication of culture
Brown sugar is added in clear water and is configured to brown sugar water, brown sugar water is boiled into disinfection, when being subsequently cooled to 30 DEG C, into brown sugar water
Microbiological feed leavening is added, seals shake culture 6-12h at 30-32 DEG C, activation bacterium solution is made；
2) mix is prepared
Granular winter jujube inferior fruit and brewex's grains will be crushed to, mix is made after mixing；
3) corn flour is added
Corn flour is added in step 1) activation bacterium solution obtained, is added into activation bacterium solution and boils clear water after cooling, mixed
It is added in mix made from step 2) after closing uniformly；
4) humidity is adjusted
Supplement clear water reaches the 60-65% of amount of dry matter to total amount of water, and humidity control is being held one at sticking together, firmly held not
The degree of water outlet is to get fermentation material；
5) it ferments
By fermentation material anaerobic fermentation 5-10 days at 16-30 DEG C, when measuring fermentation material pH less than 4.0, and clump count reaches 1,000,000,000
When cfu/g, fermentation material has alcohol ester sour taste, terminates fermentation and obtains ferment bacterium fuel.
7. a kind of preparation side using winter jujube inferior fruit preparation ruminant ferment bacterium fuel according to claim 6
Method, it is characterised in that the weight ratio of both microbiological feed leavening, brown sugar water described in step 1) actication of culture is (0.3-
0.5): 1, the concentration of brown sugar water is 10%.
8. a kind of preparation side using winter jujube inferior fruit preparation ruminant ferment bacterium fuel according to claim 6
Method, it is characterised in that it is 7:2 that step 2), which prepares the mass ratio between winter jujube and brewex's grains described in mix,；It is described by winter jujube
Inferior fruit is crushed with Double-grade disintegrating mill, grinding particle size 0.1-0.3cm；It is first dried before the winter jujube fruit powder is broken, after drying again
It is crushed, moisture content is lower than 15% after drying.
9. a kind of preparation side using winter jujube inferior fruit preparation ruminant ferment bacterium fuel according to claim 6
Method, it is characterised in that it is 10:(0.0013- that step 3), which adds corn flour described in corn flour, activation bacterium solution, the weight ratio of clear water,
0.0015): (60-65)；It is described to be added to mix made from step 2) by the way of stirring while adding after mixing
In.
10. a kind of preparation side using winter jujube inferior fruit preparation ruminant ferment bacterium fuel according to claim 6
Method, it is characterised in that the flavones content after fermenting described in step 5) fermentation in ferment bacterium fuel is greater than 250mg/100g, ring
Phosphoric acid gland acid content is greater than 20mg/100g, and ascorbic content is greater than 240mg/100g；
The specific steps of the fermentation are as follows: the fermentation material mixed is packed into the PE film sealed package for having one-way exhaust breather valve
In bag, film thickness is 20-25, and charge is the 70% of packaging bag volume, is pressed and sealed, heap fermentation after packaging, a pile 3-8
Bag, summer is aeration-cooling with high-power exhaust fan, and winter stove fire or the door curtain made of cloth cover heat preservation, and control temperature is detested at 16-30 DEG C
Aerobe fermentation 5-10 days.
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| CN106333109A (en) * | 2016-10-12 | 2017-01-18 | 盛扬 | Method for utilizing fresh jujube fermentation to prepare pig feed |
| CN110295154A (en) * | 2019-06-11 | 2019-10-01 | 海南久纳生物科技开发有限公司 | A kind of fruit ferment leavening |
| CN113973978A (en) * | 2021-09-07 | 2022-01-28 | 塔里木大学 | Red date cottonseed meal fermented feed capable of effectively reducing content of free gossypol and preparation method thereof |
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| CN113973978A (en) * | 2021-09-07 | 2022-01-28 | 塔里木大学 | Red date cottonseed meal fermented feed capable of effectively reducing content of free gossypol and preparation method thereof |
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