CN105614069A - Cassava residue bio-polypeptide fertilizer additive and preparation method thereof - Google Patents

Cassava residue bio-polypeptide fertilizer additive and preparation method thereof Download PDF

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Publication number
CN105614069A
CN105614069A CN201610079014.6A CN201610079014A CN105614069A CN 105614069 A CN105614069 A CN 105614069A CN 201610079014 A CN201610079014 A CN 201610079014A CN 105614069 A CN105614069 A CN 105614069A
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grams
additive
biological
gram
molasses
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CN201610079014.6A
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曾清平
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广州柏涵琦农业科技有限公司
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The invention discloses a cassava residue bio-polypeptide fertilizer additive and a preparation method thereof. The bio-polypeptide fertilizer additive per 1 kg contains the following components: 650-800 g of cassava residue, 40-120 g of broken rice, 70-150 g of molasses, 0.8-2 g of Saccharomyces cerevisiae, 0.8-2 g of Enterococcus faecalis, and the balance of mixture of rice chaff and husk. The preparation method comprises: stirring the materials according to the above ratio, adding suitable water, and mixing well; packaging with fermented fertilizer bags, and fermenting at 25-35 DEG for 2-3 days or fermenting at 10-20 DEG C for 4-6 days to obtain the bio-polypeptide fertilizer additive. The cassava residue bio-polypeptide fertilizer additive has good taste, can significantly improve palatability of cassava residue, and increase feed intake and speed; feed nutrients can be significantly increased and can be converted into various completely nutritional effective amino acid components necessary to animals; feed digestion, absorption and utilization rate can be increased, producing performance is improved, and 10-25% of feed can be saved by long-term use of the additive.

Description

A kind of manioc waste biological polypeptide feed additive and preparation method thereof
Technical field
Present invention relates particularly to a kind of manioc waste biological polypeptide feed additive and preparation method thereof.
Background technology
In Guangxi, Guangdong, the province such as Hainan and surrounding countries such as Vietnam, Cambodia etc. have substantial amounts of starch factory, a lot of manioc wastes can be produced when carrying out processing starch with Maninot esculenta crantz.. The main component of manioc waste is starch and cellulose, a small amount of protein. Many raisers often are used for manioc waste to make feedstuff.
As a rule, raiser manioc waste is mixed with some feedstuffs boil after feed pigs. Simple manioc waste feeds pigs or manioc waste and feedstuff hodgepodge is fed pigs, and this feedstuff mouthfeel is poor, though the belly of pig can be crammed, but it is extremely low for the nutrition digestion absorbance of manioc waste itself to be because pig, can not play the effect of the growth promoting pig; Long-term Feeding can cause pig poor growth, staple length, not only wastes time and energy, and loses more than gain.
In addition, many raisers will not store manioc waste, simply manioc waste being deposited in outside thin film simply to cover, manioc waste reforms into yellow, black within a couple of days, causes that animal is poisoning, morbidity is frequent after utilizing this manioc waste feeding animals polluted by flavacin through often.
Having part raiser to adopt saccharifying enzyme that manioc waste is carried out fermentation and prepare feedstuff: owing to saccharifying enzyme strain is single, vigor is on the low side, therefore transformation time is long, DeGrain, high expensive, and processing one ton of manioc waste needs saccharifying enzyme to pay wages 30��60 yuan. So limiting application and the popularization of this method.
How cost-effectively manioc waste making cheap, that source is wide again being become mouthfeel good, the comprehensive feedstuff of nutritive value or feed additive are significant.
Summary of the invention
It is an object of the invention to provide a kind of biological polypeptide feed additive and preparation method thereof.
The technical solution used in the present invention is:
A kind of biological polypeptide feed additive, wherein, containing following component in every 1 kilogram of biological additive of polypeptide feed: manioc waste 650��800 grams, crack rice 40��120 grams, 70��150 grams of molasses, saccharomyces cerevisiae 0.8��2 gram, enterococcus faecalis 0.8��2 gram, surplus be TONGKANG.
Preferably, containing following component in every 1 kilogram of biological additive of polypeptide feed: manioc waste 700��800 grams, crack rice 50��100 grams, 80��120 grams of molasses, saccharomyces cerevisiae 0.8��1.5 gram, enterococcus faecalis 0.8��1.5 gram, surplus be TONGKANG.
Preferably, containing following component in every 1 kilogram of biological additive of polypeptide feed: manioc waste 700��780 grams, crack rice 70��100 grams, 80��120 grams of molasses, saccharomyces cerevisiae 0.8��1.2 gram, enterococcus faecalis 0.8��1.2 gram, surplus be TONGKANG.
Preferably, containing following component in every 1 kilogram of biological additive of polypeptide feed: manioc waste 700 grams, crack rice 100 grams, 100 grams of molasses, saccharomyces cerevisiae 1 gram, enterococcus faecalis 1 gram, surplus be TONGKANG.
Preferably, containing following component in every 1 kilogram of biological additive of polypeptide feed: manioc waste 720 grams, crack rice 70 grams, 110 grams of molasses, saccharomyces cerevisiae 1 gram, enterococcus faecalis 1 gram, surplus be TONGKANG.
Preferably, containing following component in every 1 kilogram of biological additive of polypeptide feed: manioc waste 730 grams, crack rice 50 grams, 120 grams of molasses, saccharomyces cerevisiae 1 gram, enterococcus faecalis 1 gram, surplus be TONGKANG.
Preferably, containing following component in every 1 kilogram of biological additive of polypeptide feed: manioc waste 740 grams, crack rice 80 grams, 80 grams of molasses, saccharomyces cerevisiae 1 gram, enterococcus faecalis 1 gram, surplus be TONGKANG.
Preferably, containing following component in every 1 kilogram of biological additive of polypeptide feed: manioc waste 750 grams, crack rice 50 grams, 100 grams of molasses, saccharomyces cerevisiae 1 gram, enterococcus faecalis 1 gram, surplus be TONGKANG.
Preferably, biological polypeptide feed additive is additionally added a small amount of cellulose complex enzyme, and addition is 25 g ton.
The preparation method of a kind of biological polypeptide feed additive, comprises the following steps:
1) according to the described proportioning of any of the above-described item by manioc waste, crack rice, molasses, saccharomyces cerevisiae, enterococcus faecalis, TONGKANG and cellulose complex enzyme are stirred, and add suitable quantity of water, Homogeneous phase mixing;
2) with fermented feed bag pack dress, ferment 2��3 days during temperature 25 DEG C��35 DEG C, or temperature 10��20 DEG C is fermented 4��6 days namely to produce and obtained biological polypeptide feed additive.
Preferably, in step 1), saccharomyces cerevisiae, enterococcus faecalis use molasses water to carry out actication of culture in advance, are cultured to exponential phase, are then added according to viable count quality.
Preferably, when in step 1), saccharomyces cerevisiae, enterococcus faecalis molasses water carry out actication of culture, in molasses water, molasses are (3-5) with the mass ratio of water: (15-20).
Preferably, adjusting biodiversity percentage composition in step 1) is 23��27%.
The fermented feed bag of the present invention has the guarantee highly active life breath device of microorganism---" respiratory membrane ", and it can make viable bacteria (mainly yeast and lactic acid bacteria) can keep its high activity for a long time when packaging bag. Just packing after raw material inoculation, fermentation carries out in packaging bag. The gas that microorganism produces during the fermentation is discharged by respiratory membrane after reaching setting pressure, but the air in the external world can not enter in packaging bag all the time. This unidirectional exhaust apparatus ensure that the anaerobic in packaging bag and the environment without living contaminants, not only ensure that the activity of microorganism, also ensure that product can long-term accumulating simultaneously. This first packaging, the innovating technologies of after fermentation, solve the heat radiation of microorganism solid fermentation, anaerobism controls dexterously, and the difficult problem such as packaging, accumulating, stability.
The biological polypeptide feed additive of the present invention can partly substitute complete feed. First a small amount of by adopting, the principle slowly increased, start by 10% s, slowly increase feeds, and the highest can use 70%.
The invention has the beneficial effects as follows:
1) the manioc waste biological polypeptide feed additive mouthfeel of the present invention is good, the palatability of manioc waste can be significantly improved, improve feed intake and speed, amino acids oral-liquor, Yoghourt form one the same as the composition in medicated beer that microbial cells Argine Monohydrochloride therein, lactic acid bacteria, yeast are drunk by animal as people are had a liking for, and like searching for food;
2) dramatically increase feed nutrition composition, the comprehensive Available Amino Acids composition of multiple nutrients necessary to animal can be changed into;
3) feed digestion absorption rate is improved, improve and produce performance, containing multiple beneficial microorganism live bacteria, set up microecological balance in animal intestinal, feed nutrition composition therein is fully absorbed utilization by animal, can make protein, energy, mineral utilization rate reach 95%, greatly improve roughage absorption rate, therefore can reducing feed cost, life-time service can save 10-25% feedstuff;
4) improve immunity, prevent and treat intestinal tract disease, set up intestinal microecology balance, it is suppressed that the breeding of harmful levels of pathogens, increase beneficial microbe breeding;
5) fly is driven in deodorization, reduces and pollutes, and controls bacterial disease, the excretion of nitrogen in feces, phosphorus, calcium can be reduced, reduce stool odor and noxious gas emission, show as animal wastes stink and progressively alleviate, reduce feed protein and be decomposed into ammonia waste, thus reducing environmental pollution;
6) meat, eggs and milk quality is improved, produce " green meat ", " farmers''s egg ", " antibiotic-free milk ", this product is by strengthening digestive and absorptive functions, fully absorb the natural pigment utilizing feedstuff Middle nutrition composition and raw material, without adding harmful and affecting the chemical pigment tonyred of animal products natural edible local flavor, Carophyll red, the livestock meat that the rural area corn that can match in excellence or beauty is fed. The natural increase animal product degree of staining of energy and edible flavor, pig skin is ruddy, and hair color is shinny; Broiler meat-type duck color burn; Improving quality and the color of eggshell, Ovum Gallus domesticus album thickness is thick, and egg yolk is scarlet; Aquatic animal color is more healthy, immaculate.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further illustrated, but is not limited thereto.
Embodiment 1
A kind of manioc waste biological polypeptide feed additive, contains in every 1 kilogram of manioc waste biological polypeptide feed additive: manioc waste 720 grams, crack rice 80 grams, 100 grams of molasses, saccharomyces cerevisiae 1 gram, enterococcus faecalis 1 gram, surplus be TONGKANG. Additionally, above-mentioned manioc waste biological polypeptide feed additive per ton adds 25g cellulose complex enzyme.
Preparation method is as follows:
1) it is (3-5) by molasses and 35-40 DEG C of warm water according to mass ratio: the culture fluid that (15-20) is mixed to get, saccharomyces cerevisiae, enterococcus faecalis strain are carried out actication of culture respectively, it is cultured to exponential phase, obtain work seed strain liquid, calculate viable count, be then centrifuged for obtaining thalline;
2) work thalline obtained above is pressed saccharomyces cerevisiae, the every kilogram of feed of enterococcus faecalis (viable bacteria) each 1g/, with manioc waste, crack rice, molasses, TONGKANG, cellulose complex enzyme mixture stirring, adjust biodiversity percentage composition and be approximately in about 25%, Homogeneous phase mixing;
3) with special fermented feed bag pack dress, natural fermentation 2��3 days during temperature 25 DEG C��35 DEG C, or during temperature 10��20 DEG C, natural fermentation production in 4��6 days obtains manioc waste biological polypeptide feed additive.
The present invention is fermented after utilizing special fermented feed bag pack dress again: during beginning, in saccharomyces cerevisiae consumption bag, residual oxygen carries out breeding and respiratory metabolism, also creates an anaerobiosis environment for enterococcus faecalis simultaneously. Then, saccharomyces cerevisiae carries out glycolysis under anaerobic, produces ethanol and carbon dioxide, and enterococcus faecalis is also bred simultaneously, metabolism, produces organic acid.
Embodiment 2
A kind of manioc waste biological polypeptide feed additive, contains in every 1 kilogram of manioc waste biological polypeptide feed additive: manioc waste 720 grams, crack rice 70 grams, 110 grams of molasses, saccharomyces cerevisiae 1 gram, enterococcus faecalis 1 gram, surplus be TONGKANG. Additionally, above-mentioned manioc waste biological polypeptide feed additive per ton adds 25g cellulose complex enzyme. Manioc waste biological polypeptide feed additive is prepared according to the method for embodiment 1.
Embodiment 3
A kind of manioc waste biological polypeptide feed additive, contains in every 1 kilogram of manioc waste biological polypeptide feed additive: manioc waste 730 grams, crack rice 50 grams, 120 grams of molasses, saccharomyces cerevisiae 1 gram, enterococcus faecalis 1 gram, surplus be TONGKANG. Additionally, above-mentioned manioc waste biological polypeptide feed additive per ton adds 25g cellulose complex enzyme. Manioc waste biological polypeptide feed additive is prepared according to the method for embodiment 1.
Embodiment 4
A kind of manioc waste biological polypeptide feed additive, contains in every 1 kilogram of manioc waste biological polypeptide feed additive: manioc waste 740 grams, crack rice 80 grams, 80 grams of molasses, saccharomyces cerevisiae 1 gram, enterococcus faecalis 1 gram, surplus be TONGKANG. Additionally, above-mentioned manioc waste biological polypeptide feed additive per ton adds 25g cellulose complex enzyme. Manioc waste biological polypeptide feed additive is prepared according to the method for embodiment 1.
Embodiment 5
A kind of manioc waste biological polypeptide feed additive, contains in every 1 kilogram of manioc waste biological polypeptide feed additive: manioc waste 750 grams, crack rice 50 grams, 100 grams of molasses, saccharomyces cerevisiae 1 gram, enterococcus faecalis 1 gram, surplus be TONGKANG. Additionally, above-mentioned manioc waste biological polypeptide feed additive per ton adds 25g cellulose complex enzyme. Manioc waste biological polypeptide feed additive is prepared according to the method for embodiment 1.
Culture experiment one
1) test period and place
Test and carry out at Nansha District, Guangzhou poultry farms 12 days��July 13 April in 2015.
2) materials and methods
Sanhuang chicken is 28 age in days stalwartness chickens. Broiler is randomly divided into 4 process, and each process repeats for 4 times, each repetition 30 plumage. Wherein, process l: feed autogamy without any antibiotic complete feed, process 2: in daily grain of chicken, add the manioc waste feed additive that 10% embodiment 1 prepares, process 3: in daily grain of chicken, add the fermentation cassava dreg fodder additive that 20% embodiment 1 prepares, process 4: in daily grain of chicken, add the fermentation cassava dreg fodder additive that 30% embodiment 1 prepares.
3) feeding and management
Feeding manner is that ground is raised scattered, free choice feeding, drinking-water.
4) project is measured
Performance test: in 56,91,120 ages in days randomly draw from each process 20 plumage broiler carry out weighing, ramming material, calculate the average daily feed consumption rate of broiler, Average weight increasing a day and feedstuff-meat ratio. Observe each process appetite of chicken, health and death condition.
5) experimental result and analysis
Result is in Table 1 and table 2.
Table 1: add fermentation cassava dreg fodder Additive On Chickens in daily ration and produce the impact of performance
Data according to table 1, by whole test it can be calculated that process 2,3,4 and compare with processing 1() compare, Average weight increasing a day has been respectively increased 7.02%, 6.95% and 11.18%, and feed intake has been respectively increased 5.95%, 5.82% and 11.11%. In feedstuff-meat ratio, process 2,3,4 ratio comparison reductions by 1%, 1%, 1%; In sickness rate, process 2,3,4 than comparison reduce respectively l2%, 6% and 6%. Comprehensive indices it can be seen that add fermentation cassava dreg fodder additive in daily grain of chicken, it is possible to reduces aquaculture cost, improve survival rate and economic benefit.
By its economic benefit of whole analysis of experiments, result is in Table 2.
Table 2 Economic and Efficiency Analysis
As shown in Table 2: calculate by feedstuff 2.5 yuan/kilogram, fermentation cassava slag additive 2.0 yuan/kilogram, process 2,3,4 with process 1(compare) gross profit be 4.33,4.47,4.76 and 3.33 yuan/plumage respectively, the gross profit processing 2,3,4 has been respectively increased 30.03%.34.23% and 42.94% than comparison, significant difference. Visible, fermentable manioc waste feed additive feeding dorking, it is possible to reduce aquaculture cost, improve survival rate and economic benefit.
In sum, the present invention adds 10%, 20% or 30% fermentable manioc waste feed additive in daily ration of broiler, be conducive to improving the Average weight increasing a day of broiler, feed intake, reduce mortality rate, sickness rate, feedstuff-meat ratio, it is possible to reduce aquaculture cost, improve survival rate and economic benefit.
To the Sanhuang chicken processing 2-4 Sanhuang chicken and the normal diet obtained cultivation, and rural area is raised the Sanhuang chicken of feeding grain scattered and is carried out meat, eggs and milk quality contrast experiment.
Result shows: Sanhuang chicken (processing 2-4) the meat, eggs and milk quality that manioc waste biological polypeptide feed additive of the present invention feeds significantly improves, and is better than the Sanhuang chicken that normal diet is fed. It is embodied in: Carnis Gallus domesticus color is relatively deep, and yellowly, the Sanhuang chicken quality raising feeding grain with rural area scattered is suitable. Meanwhile, the produced egg of Sanhuang chicken of the present invention, quality and the color of eggshell improve, and Ovum Gallus domesticus album thickness is thick, and egg yolk is scarlet. Use biological polypeptide feedstuff after 60 days; Average egg weight increases 1--3 gram, egg-laying peak growth; Laying period life-time service, minimum increase laying period 1--2 month, on average every chicken fecund 4--5 jin egg are additionally, Sanhuang chicken is not easily sick superseded, and feather light increases the price sold.
Culture experiment two
1) test period and place
Tested in June, 2015 on Heshan pig farm, Jiangmen.
2) materials and methods
To 3000 sow breeding stocks, contrast effect:
Select piglet 120, point matched group and each 60 of experimental group; Pig 120 in selection, point matched group and each 60 of experimental group; Select big porker 120, point matched group and each 60 of experimental group.
Matched group selects bean pulp type (add consumption respectively 12%, 10%, 8%) as daily ration, the experimental group biological polypeptide feedstuff replacement complete feedstuff of 10%, 15%, 20% embodiment 2.
3) experimental result:
3.1) the piglet stage:
Experimental group: pig end average weight is: 21.52kg, average daily gain 467.7g, and feed consumption rate is 19.79kg, and feed-weight ratio is 1.42:1;
Matched group: pig end average weight is: 20.08kg, average daily gain 420.7g, and feed consumption rate is 19.05kg, and feed-weight ratio is 1.51:1;
Experimental result data shows: experimental group reduces by 6% at piglet stage food consumption, and average daily gain improves 47g, improves 11.17%, and diarrhea rate reduces by 6.6%, and overall incidence reduces by 13.14%, and feedstuff-meat ratio heavily improves 0.09, reduces food consumption 6%.
3.2) the pig stage in:
Experimental group: pig end average weight is: 48.46kg, average daily gain: 607g, and feed consumption rate is 40.97kg, and feed-weight ratio is: 2.25:1;
Matched group: pig end average weight is: 47.10kg, average daily gain: 525g, and feed consumption rate is 39.85kg, and feed-weight ratio is: 2.53:1;
Experimental result data shows: experimental group is in the middle pig stage, and daily gain improves 15.62%, and feed-weight ratio improves 0.28, reduces food consumption and is: 11.11%.
3.3) the big porker stage:
Experimental group: pig end average weight is: 86.9kg, average daily gain 890g, feed consumption 72.2kg, and feed-weight ratio is: 2.7:1;
Matched group: pig end average weight is: 86.7kg, average daily gain 840g, feed consumption 73.42kg, and feed-weight ratio is: 2.9:1;
Experimental data shows: experimental group improves 50g in the average daily gain of big porker stage, improves 10%, and feed-weight ratio improves: 0.2, reduces food consumption 2%.
Found that: compared with the pig without biological polypeptide feed additive, after the pig (including piglet, middle pig and big porker) of experimental group uses biological polypeptide feedstuff 3��5 days, it has been found that the appetite of pig is more vigorous; Using biological polypeptide feedstuff after 7��10 days, the stool colour of pig deepens, feces is bulk, niblet reduces or disappears, colony house ammonia concentration reduces; Using biological polypeptide feedstuff after 20��30 days, the skin of pig is ruddy, bright in color; By comparing, use the biological polypeptide feed additive of the present invention, it is possible to shorten pig and deliver the cycle for sale 6-12 days.
So, use the biological polypeptide feed additive of the present invention, it is possible to reduce feedstuff-meat ratio, raising survival rate, enhancing immunity and premunition, shorten and deliver the cycle for sale.
Culture experiment three
Biological polypeptide feed additive embodiment 3 prepared uses foster goose: the feeding method being directly incorporated in complete feed.
Namely directly use complete feed, coordinate the feeding method of a small amount of biological polypeptide feedstuff:
0��3 week old: complete feed 80%, biological polypeptide feed additive 20%;
4��8 week old: complete feed 70%, biological polypeptide feed additive 30%;
It is bred as goose to listing: complete feed 70%, biological polypeptide feed additive 30%.
Wherein biological polypeptide feedstuff 2 yuan/kilogram, about 2.8 yuan/kilogram of goose complete feed.
First a small amount of by adopting, the principle slowly increased, start by 10%, slowly increase and feed.
During kind of a goose stops egg, it is possible to that strengthens biological polypeptide feedstuff makes consumption, the highest can use 70%.
Being cultivated by said method, after reaching to deliver requirement for sale, statistics feeds cost, it has been found that: every meat goose can increase economic benefit more than 5 yuan, delicious meat, plants goose and saves feedstuff every year and pay wages more than 20 yuan.
Nutrious fermented composition measurement is tested
Manioc waste biological polypeptide feed additive is measured at the composition of fermentation front and back, obtains product for embodiment 1. Result is in Table 3 and 4.
The change before and after fermentation of table 3 vitamin content
(data above is measurement result after drying, and unit is mg/kg).
The change table of table 4 nutritional labeling
From table 3 and 4: after fermentation, manioc waste biological polypeptide feed additive Middle nutrition composition there occurs huge change, although apparent protein content relative value increases seldom, but the digestibility of protein adds several times more than, crude protein content adds more than 50% relatively, digestible energy and metabolizable energy significantly increase, additionally, the content of vitamin is multiplied, nutritive value increases substantially.
The biological polypeptide feed additive being main matter with manioc waste after inventive formulation and preparation method process, nutrition therein has had and has been significantly increased, sugar, albumen, aminoacid, vitamin significantly improve, feeding effect is fairly obvious, as can be seen from the above table, changing relatively larger has crude fibre, than reduces more than 56% before processing, reduces more than half. This crude fibre being possibly due to manioc waste presents more open structure, is jointly cooperated so that fibre structure is more loose and exposes by enzyme in this formula and microorganism, and fibre structure interstitial is digested, is conducive to the cooperation enzymolysis of cellulose complex enzyme. Notable change additionally is digestible protein increment rate be quintupling increase, this is an of paramount importance change, the raising of nutritive value is played conclusive effect: the protein digestibility before degraded is about 30% (note: protein digestibility is that digestible protein is divided by crude protein content, here it is 1.2% and obtains 30% divided by 4.01%), protein digestibility after degraded is 89.6%(note: protein digestibility is that digestible protein is divided by crude protein content, here it is 14.8% and obtains 89.6% divided by 16.51%), improve 3 times. In actual breeding production, protein digestibility has more the meaning of reality than protein content
Product composition test experience
The manioc waste biological polypeptide feed additive that the present invention (embodiment 2) is prepared send China Guangzhou Analysis &. Test Center that its composition is detected, and result is as follows:
Table 5 manioc waste biological polypeptide feed additive protein component is analyzed
Table 6 manioc waste biological polypeptide feed additive component analysis
(wherein, total number of bacteria includes lactic acid bacteria and saccharomyces cerevisiae and miscellaneous bacteria)
From table 5 and 6: the manioc waste biological polypeptide feed additive of the present invention contains abundant nutrition, including crude fat, crude protein, various hydrolysis amino acid. In addition containing substantial amounts of saccharomyces cerevisiae and lactic acid bacteria, wherein with lactic acid bacteria for dominant microflora, shelf life of products of the present invention is 1 year. In addition mycete rhzomorph, product safety environmental protection of the present invention are not detected.
Above-described embodiment is the present invention preferably embodiment; but embodiments of the present invention are also not restricted to the described embodiments; the change made under other any spirit without departing from the present invention and principle, modification, replacement, combination, simplification; all should be the substitute mode of equivalence, be included within protection scope of the present invention.

Claims (10)

1. a biological polypeptide feed additive, it is characterized in that, containing following component in every 1 kilogram of biological additive of polypeptide feed: manioc waste 650��800 grams, crack rice 40��120 grams, 70��150 grams of molasses, saccharomyces cerevisiae 0.8��2 gram, enterococcus faecalis 0.8��2 gram, surplus be TONGKANG.
2. biological polypeptide feed additive according to claim 1, it is characterized in that, containing following component in every 1 kilogram of biological additive of polypeptide feed: manioc waste 700��800 grams, crack rice 50��100 grams, 80��120 grams of molasses, saccharomyces cerevisiae 0.8��1.5 gram, enterococcus faecalis 0.8��1.5 gram, surplus be TONGKANG.
3. biological polypeptide feed additive according to claim 1, it is characterized in that, containing following component in every 1 kilogram of biological additive of polypeptide feed: manioc waste 700��780 grams, crack rice 70��100 grams, 80��120 grams of molasses, saccharomyces cerevisiae 0.8��1.2 gram, enterococcus faecalis 0.8��1.2 gram, surplus be TONGKANG.
4. biological polypeptide feed additive according to claim 1, it is characterized in that, containing following component in every 1 kilogram of biological additive of polypeptide feed: manioc waste 700 grams, crack rice 100 grams, 100 grams of molasses, saccharomyces cerevisiae 1 gram, enterococcus faecalis 1 gram, surplus be TONGKANG.
5. biological polypeptide feed additive according to claim 1, it is characterized in that, containing following component in every 1 kilogram of biological additive of polypeptide feed: manioc waste 720 grams, crack rice 70 grams, 110 grams of molasses, saccharomyces cerevisiae 1 gram, enterococcus faecalis 1 gram, surplus be TONGKANG.
6. the biological polypeptide feed additive according to any one of claim 1-5, it is characterised in that biological polypeptide feed additive per ton adds 25g cellulose complex enzyme.
7. the preparation method of a biological polypeptide feed additive, it is characterised in that comprise the following steps:
Described in any one of claim 1-6 proportioning by manioc waste, crack rice, molasses, saccharomyces cerevisiae, enterococcus faecalis, TONGKANG and cellulose complex enzyme are stirred, and add suitable quantity of water, Homogeneous phase mixing;
Fill with fermented feed bag pack, ferment 2��3 days during temperature 25 DEG C��35 DEG C, or temperature 10��20 DEG C is fermented 4��6 days namely to produce and obtained biological polypeptide feed additive.
8. preparation method according to claim 7, it is characterised in that: in step 1), saccharomyces cerevisiae, enterococcus faecalis use molasses water to carry out actication of culture in advance, are cultured to exponential phase, are then added according to viable count quality.
9. the preparation method according to claim 7 or 8, it is characterised in that: when in step 1), saccharomyces cerevisiae, enterococcus faecalis molasses water carry out actication of culture, in molasses water, molasses are (3-5) with the mass ratio of water: (15-20).
10. preparation method according to claim 7, it is characterised in that: adjusting biodiversity percentage composition in step 1) is 23��27%.
CN201610079014.6A 2016-02-04 2016-02-04 Cassava residue bio-polypeptide fertilizer additive and preparation method thereof CN105614069A (en)

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