CN106834163A - Feed fermentation agent, fermented feed and preparation method thereof - Google Patents

Feed fermentation agent, fermented feed and preparation method thereof Download PDF

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CN106834163A
CN106834163A CN201611191373.7A CN201611191373A CN106834163A CN 106834163 A CN106834163 A CN 106834163A CN 201611191373 A CN201611191373 A CN 201611191373A CN 106834163 A CN106834163 A CN 106834163A
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feed
fermentation
raw material
fermented
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江伟华
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Hunan Saifu Resource Fodder Technology Co Ltd
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Abstract

The invention provides a kind of feed fermentation agent,Fermented feed and preparation method thereof,It is related to livestock products cultural technique field,Including a kind of feed fermentation agent,The leavening is main by Lactobacillus plantarum,Enterococcus faecalis,Bacillus subtilis,Saccharomyces cerevisiae,Lactobacillus cellobiosas and complex enzyme formulation etc. are constituted,And fermented feed prepared by the production method fermented in the device of one-way exhaust using the feed fermentation agent and fermentation raw material,Solve existing livestock culture feed nutritive value general,Animal digestion absorptivity is not high to be abused with antibiotic medicine in cultivation,Cause long the time required to livestock and poultry cultivation high cost and the fermentation of existing fermented feed,The slow problem of fermentation starting,The nutritional ingredient and sorption enhanced rate for having reached fermented feed are high,The intestinal health of animal can be protected in feed containing substantial amounts of probiotics,And then improve animal body immunity,Reduce the use of antibiotic in livestock culture.

Description

Feed fermentation agent, fermented feed and preparation method thereof
Technical field
The present invention relates to livestock products cultural technique field, more particularly, to a kind of feed fermentation agent, fermented feed and its preparation Method.
Background technology
The feed that traditional aquaculture is used generally corn, dregs of beans, wheat bran etc., the nutritive value of these feeds are general, need Measure greatly, animal digestion absorbability is not high, and the feed cost of plant is high.Growing with aquaculture, people and animals strive grain Contradiction it is more and more severeer.
Meanwhile, antibiotic is largely used in traditional aquaculture causes bacterium to produce drug resistance, animal gastrointestinal tract normal flora Imbalance and immunity of organisms decline, and livestock and poultry endogenous infection and suprainfection easily occur, and animal-breeding ecological environment is subject to very big Negative effect.Further, since the abuse of antibiotic etc, causes Immune Function In Animals and efficiency of feed utilization to decline, livestock and poultry are supported The increase of middle medicine and feed cost input is grown, medicament residue also limit the foreign exchange earning of animal product, make animal product matter Amount declines.
Above-mentioned these problems eventually affect the economic benefit of animal productiong again, cause livestock and poultry cultivation productive profit space It is less and less.Therefore, a kind of unconventional water resources resource is developed, is used to improve feed conversion conversion ratio, quick startup fermentation, is shortened The time required to fermentation, raising animal body immunologic function, and then the use of antibiotic is reduced, can yet be regarded as one can promote animal husbandry Health, the effective way of sustainable development.
In view of this, it is special to propose the present invention.
The content of the invention
The first object of the present invention is to provide a kind of feed fermentation agent, and above-mentioned feed fermentation agent has feed conversion rate Height, fermentation starting is fast, and the total time needed for fermentation is short and can suppress the advantage of the outer growth and field planting for attacking bacterium in fermentation.
The second object of the present invention is to provide a kind of fermented feed, and the fermented feed has rich in nutrition content, agreeable to the taste Property good and feed conversion rate advantage high, meanwhile, substantial amounts of beneficial viable bacteria is rich in fermented feed, be beneficial to microbial population of animal intestinal tract Balance, with preventing and treating livestock and poultry alimentary tract flora imbalance, raising animal body immunologic function, and then reduces the use of antibiotic, makes Animal products drug residue free, does not produce drug resistance, quality safety to realize high quality and favourable price, its economic benefit highly significant.
The third object of the present invention is to provide a kind of preparation method of fermented feed, and the method is solved for a long time well Perplex the problem of the contradiction flora coexistence of field of microbial fermentation.First aerobic fermentation, is then carried out in the device of one-way exhaust Amphimicrobian ferments, finally the production technology based on anaerobic fermentation, and total time needed for fermentation is short, and fermentation process is more abundant, mould More thoroughly, the beneficial metabolic product of accumulation more enriches so that fermented feed of the present invention for verticillium toxin and ANFs degraded Nutritive value has obtained significantly being lifted very much.
A kind of feed fermentation agent that the present invention is provided, above-mentioned leavening is mainly made up of the raw material of following weight portion：Plant 20~25 parts of lactobacillus, 15~20 parts of enterococcus faecalis, 10~15 parts of bacillus subtilis, 5~10 parts of saccharomyces cerevisiae, cellobiose 15~25 parts of 10~15 parts of lactobacillus and complex enzyme formulation.
Further, above-mentioned leavening is mainly made up of the raw material of following weight portion：21~23 parts of Lactobacillus plantarum, excrement intestines 16~18 parts of coccus, 11~14 parts of bacillus subtilis, 6~8 parts of saccharomyces cerevisiae, 11~14 parts of lactobacillus cellobiosas and compound 18~22 parts of enzyme preparation.
Further, above-mentioned leavening is mainly made up of the raw material of following weight portion：23 parts of Lactobacillus plantarum, enterococcus faecalis 20 parts of 18 parts, 13 parts of bacillus subtilis, 8 parts of saccharomyces cerevisiae, 13 parts of lactobacillus cellobiosas and complex enzyme formulation.
Further, above-mentioned complex enzyme formulation is protease, cellulase, zytase, mannonase galactoside Two or more mixing composition in enzyme and glucose oxidase；
Preferably, above-mentioned complex enzyme formulation includes following components in percentage by weight：Protease 5~15%, cellulase 20~30%, zytase 15~20%, mannase 15~20%, galactosidase 25~30% and glucose oxidase 5~10%.
Further, above-mentioned protease by neutral proteinase and acid protease with 1：1 weight is than uniform mixing composition.
What the present invention was provided, a kind of fermented feed, above-mentioned fermented feed is by the above-mentioned feed fermentation of above-mentioned 1~5 any one Agent fermentation is obtained.
What the present invention was provided, the preparation method of above-mentioned fermented feed, the above method comprises the following steps：
(1), above-mentioned feed fermentation agent is put into fermentation raw material and is inoculated with and is well mixed, be subsequently added water by after inoculation The water content of fermentation raw material adjust to 30~50%, fermentation raw material to be fermented is obtained；
(2), above-mentioned steps (1) fermentation raw material to be fermented is fitted into one-way exhaust equipment and is fermented, 30~40 Fermented 3~7 days at DEG C, obtain fermented feed.
Further, above-mentioned fermentation raw material is one kind of dregs of beans, corn, wheat bran, rice bran meal, manioc waste and corn germ cake Or several mixing；
Preferably, above-mentioned fermentation raw material is made up of dregs of beans and wheat bran, and the weight ratio of both dregs of beans and wheat bran is 4：1；
Preferably, above-mentioned fermentation raw material is made up of dregs of beans, corn and wheat bran, dregs of beans, three kinds of weights of raw material of corn and wheat bran Amount is than being 4：5：1；
Preferably, above-mentioned fermentation raw material is made up of wheat bran, rice bran meal, maize peel and corn germ cake, wheat bran, rice bran meal, The weight ratio of four kinds of raw materials of maize peel and corn germ cake is 1：2：1：3；
Preferably, above-mentioned fermentation raw material is made up of dregs of beans, corn, wheat bran, rice bran meal, manioc waste and corn germ cake, beans The dregs of rice, corn, wheat bran, rice bran meal, the weight ratio of six kinds of raw materials of manioc waste and corn germ cake are 6：4：5：5：5：5.
Further, above-mentioned leavening and the weight ratio of fermentation raw material are 1~3：1000.
Further, above-mentioned one-way exhaust equipment is respiratory membrane solid-state fermentation bag or the sealing container with one-way exhaust valve.
Compared with prior art, beneficial effects of the present invention are：
(1) leavening that the present invention is provided, can turn the crude fibre and crude protein in fermentation raw material during the fermentation Turning to can be by the albumen of animal use, the ANFs in degradable fermented raw material, while protease, fat in increased feed Fat enzyme, cellulolytic enzyme and multivitamin content, improve the nutritional ingredient and sorption enhanced rate of feed.
(2) leavening that the present invention is provided, fermented bacterium collocation is reasonable in the leavening, both contains aerobic bacteria, also includes Facultative anaerobic bacteria, each strain each other have very close synergy relation, mutually promotes, improve fermentation efficiency and Effect.
(3) leavening that the present invention is provided, Lactobacillus plantarum and enterococcus faecalis can produce organic acid, wave in the leavening Hair property aliphatic acid, lactic acid, hydrogen peroxide and antibiotic property metabolite, are used to suppress the outer growth and field planting for attacking bacterium.Meanwhile, plant Lactobacillus and enterococcus faecalis can also stimulate the generation of secretory IgA, and increase the generation of interferon-γ, and lactic acid bacteria Specific and nonspecific immunocyte can also be activated.
(4) leavening that the present invention is provided, complex enzyme formulation can be as the benefit of strain extracellular proteinase in the leavening Fill, accelerate startup and the efficiency of fermentation, shorten the total time needed for completing fermentation, reduce the consumption of energy and nutrient, reduce not Good environmental microorganism infection and the risk grown, reduce mould and mycotoxin, improve the quality of fermented product, improve fermentation effect Rate and benefit.Therefore fermented feed Product quality and safety, stabilization, the high-quality for being prepared using leavening of the invention.
(5) the fermented fodder nutrient composition that the present invention is provided enriches, fragrant odour, and sour-sweet soft, moisture is high, agreeable to the taste Property it is splendid, livestock and poultry feed intake is high, digests and assimilates, and protein in feed, fat and complex carbohydrates are by fermentation in agent The degradation of hydrolase, fermenting enzyme and respiratory enzyme that produces of probiotics after, feed conversion rate is significantly improved, and also can be with The various bioactivators such as vitamin, organic acid and somatomedin are provided, accelerate growth of animals or poultry, improve Production of Livestock and Poultry Can, reduce material meat (material egg) ratio.
(6) fermented feed of the present invention is rich in substantial amounts of beneficial viable bacteria, is beneficial to microbial population of animal intestinal tract balance, is raiseeed with preventing and treating Fowl archenteric flora is lacked of proper care, and is significantly reduced grice diarrhoea and is reduced the function of prevention of sow constipation, while animal body can also be improved Immunologic function, produces various antibacterial materials, such as lactein, acidophilin, bacitracin and organic acid, hydrogen peroxide, suppression The growth and breeding of pathogen processed, so that disease preventing and treating, reduces the use of antibiotic, animal drug residue free does not produce drug resistance, Animal products quality safety, meat consolidation, it is very popular, high quality and favourable price can be realized, its economic benefit highly significant.
(7) original normal bacterium synergy in the probiotics in fermented feed of the present invention and livestock and poultry body, protects animal intestine Road health, suppresses the propagation of spoilage organisms in animal intestinal tract, so as to reduce the generation of harmful substance, reduces protein to ammonia and amine Conversion, reduces the generation of the harmful substances such as nitrosamine, ammonia, indoles, scatol, foul odour is eliminated, so as to reduce these materials By excrement pollution on the environment, air in house is purified, the growing environment of clean, sanitary pleasure is provided for livestock and poultry, together When be also advantageous for cultivating the physically and mentally healthy of people and be in a cheerful frame of mind.
(8) preparation method of the fermented feed that the present invention is provided, the method solves long-standing problem microorganism hair well The problem of the contradiction flora coexistence in ferment field.Anaerobic and the environment without living contaminants are ensure that in the device of one-way exhaust, this Aerobic bacteria in invention can quickly consume oxygen, and decline the pH of fermentation raw material, so as to detest for lactobacillus plantarum is this kind of The growth and breeding of oxygen bacterium provides good precondition, and then the organic acid for producing also is continuously increased, and macromolecular substances are continuous Degraded, the beneficial metabolic product of accumulation is more abundant so that the nutritive value of fermented feed of the present invention has been obtained very significantly Lifting.
(9) preparation method of the fermented feed that the present invention is provided, because fermented bacterium collocation is reasonable in leavening, both contained Aerobic bacteria, also including facultative anaerobic bacteria, then postvaccinal fermentation raw material first aerobic fermentation in the device of one-way exhaust is carried out Amphimicrobian ferments, finally the production technology based on anaerobic fermentation, and its fermenting speed is fast, and fermentation total time is short, and 3~7 days i.e. Fermentation can be completed, the time than general anaerobic fermentation will shorten 1/2nd or so.
Specific embodiment
Technical scheme is clearly and completely described below in conjunction with embodiment, it is clear that described reality It is a part of embodiment of the invention to apply example, rather than whole embodiments.Based on the embodiment in the present invention, the common skill in this area The every other embodiment that art personnel are obtained under the premise of creative work is not made, belongs to the model of present invention protection Enclose.
According to an aspect of the present invention, a kind of feed fermentation agent, the main raw material by following weight portion of above-mentioned leavening Composition：20~25 parts of Lactobacillus plantarum, 15~20 parts of enterococcus faecalis, 10~15 parts of bacillus subtilis, saccharomyces cerevisiae 5~10 15~25 parts of part, 10~15 parts of lactobacillus cellobiosas and complex enzyme formulation.
The function of each fermented bacterium is in feed fermentation agent of the present invention：
Lactobacillus plantarum (Lactobacillus platarum) belongs to homofermentative lactic bacteria, what its anaerobic fermentation was produced A large amount of lactic acid, lactein can suppress harmful bacteria growing, prevent mycotoxin from producing, and improve palatability, reduce animal intestinal tract pH Value, improves digestive enzyme activity, promotes to digest and assimilate.Lactobacillus plantarum as humans and animals intestines and stomach beneficial flora, with dimension Intestinal flora balance is held, immunity of organisms is improved and promotes various functions such as absorption of nutrient ingredients.Also, the plant in the present invention Thing lactobacillus derives from livestock and poultry alimentary tract, stress not, can be survived in livestock and poultry alimentary tract.
In the present invention, the above-mentioned typical but non-limiting content of Lactobacillus plantarum is：20 parts, 21 parts, 22 parts, 23 parts, 24 Part or 25 parts.
Enterococcus faecalis (Enterococcus facealis) is one of flora in humans and animals enteron aisle, can produce day Right antibiotic, is conducive to body health；The antibacterial substances such as bacteriocin can also be produced simultaneously, suppress Escherichia coli and salmonella etc. The growth of pathogen, improves intestinal microenvironment, moreover it is possible to suppress the breeding of urease-producing bacterium and spoilage organisms in enteron aisle, reduces enteron aisle Urease and endotoxic content, make ammonia and the decline of endotoxic content in blood.
In the present invention, the above-mentioned typical but non-limiting content of enterococcus faecalis is：15 parts, 16 parts, 17 parts, 18 parts, 19 parts Or 20 parts.
Bacillus subtilis (Bacillus subtilis) itself can synthesize AMS, protease, lipase, fiber The enzymes such as plain enzyme, together play a role in alimentary canal with the digestive enzymes in animal body.The withered grass of producing bacillus subtilis life Rhzomorph, polymyxins, nystatin, gramicidins isoreactivity material, the conditioned pathogen to pathogenic bacteria or autogenous infection have Obvious inhibitory action.Bacillus subtilis can rapidly consume the free oxygen in environment, cause enteron aisle hypoxemia, promote beneficial anaerobism Bacteria growing, and the organic acids such as lactic acid are produced, gut pH is further reduced, other pathogenic bacteria growings are suppressed indirectly.Withered grass bud Spore bacillus energy synthetic vitamin B1、B2、B6, various B family vitamins such as nicotinic acid, improve interferon and macrophage in animal body Activity.Bacillus subtilis is capable of growing for stimulating animal immune organ, and activation T, bone-marrow-derived lymphocyte improve immune globulin White and antibody level, strengthens cellular immunity and humoral immune function, improves herd immunity.
In the present invention, the above-mentioned typical but non-limiting content of bacillus subtilis is：15 parts, 16 parts, 17 parts, 18 parts, 19 parts or 20 parts.
Saccharomyces cerevisiae (Saccharomyces cerevisiae) is rich in vitamin B, protein and various digestive ferments, and can Production nucleic acid analog derivative, ergosterol, cromoci, coacetylase, glutathione and several amino acids are extracted from saccharomycete Deng.The mineral energies such as selenium, chromium in yeast improve the immunity of animal.After fermentation, a kind of influence calcium, magnesium, iron in fermentation bed material Phytic acid etc. element absorption can be decomposed, so as to improve absorption and utilization of the animal to these nutriments.Meanwhile, saccharomyces cerevisiae Zymase is produced, feed mycoprotein content is improved, ethanol fragrance is produced, increases feed palatability.
In the present invention, the above-mentioned typical but non-limiting content of saccharomyces cerevisiae is：15 parts, 16 parts, 17 parts, 18 parts, 19 parts Or 20 parts.
There is the collaboration mutually promoted in lactobacillus cellobiosas (Lactobacillus cellobioSuS) and cellulase Effect, can be coupled together the enzymatic saccharification of cellulose and lactic fermentation is synchronously carried out, and eliminates product (glucose, fiber Disaccharides) to the feedback inhibition of cellulase, the enzymolysis efficiency of cellulose is improved, while accelerating fibers disaccharides lactobacillus Lactic fermentation effect carrying out.
In the present invention, the above-mentioned typical but non-limiting content of saccharomyces cerevisiae is：15 parts, 16 parts, 17 parts, 18 parts, 19 parts Or 20 parts.
Complex enzyme formulation as the supplement of strain extracellular proteinase, can promote the drop of protein substrate and ANFs Solution, improves the content of the molten albumen of product acid and small peptide, shortens the total time needed for completing fermentation, reduces disappearing for energy and nutrient Consumption, reduces poor environment microorganism infection and the risk grown, and reduces mould and mycotoxin, improves the quality of fermented product, Improve fermentation efficiency and benefit.
In the present invention, the above-mentioned typical but non-limiting content of complex enzyme formulation is：15 parts, 16 parts, 17 parts, 18 parts, 19 Part or 20 parts.
Leavening of the invention, with increasing quantity of useful microbe and improving the double effectses of metabolite content, can Significantly to lift the toggle speed and product quality of solid state fermentation.Ferment-fermented strain collocation of the invention rationally, both contained There is aerobic bacteria, also including facultative anaerobic bacteria, each strain (strain) has very close synergy relation each other, mutually promotees Enter so that the fermentation of aerobic fermentation, anaerobic fermentation and amphimicrobian can be carried out simultaneously, can improve Feed Manufacturing efficiency, be saved The energy, improves the efficiency and effect of fermentation.Meanwhile, Lactobacillus plantarum and enterococcus faecalis can produce organic acid, volatility fat Fat acid, lactic acid, can suppress the outer growth and field planting for attacking bacterium, produce hydrogen peroxide and antibiotic property metabolite.Lactobacillus plantarum and Enterococcus faecalis can also stimulate the generation of secretory IgA, and increase the generation of interferon-γ, and lactic acid bacteria can also swash The specific and nonspecific immunocyte of work.
Additionally, complex enzyme formulation can accelerate fermentation as the supplement of strain extracellular proteinase in leavening of the invention Startup and efficiency, shorten the total time needed for completing fermentation, reduce the consumption of energy and nutrient, reduce poor environment microorganism Infection and the risk grown, reduce mould and mycotoxin, improve the quality of fermented product, improve fermentation efficiency and benefit.Cause This fermented feed Product quality and safety, stabilization, high-quality for being prepared using leavening of the invention.
In the preferred embodiment of the present invention, above-mentioned leavening is mainly made up of the raw material of following weight portion：Plant 21~23 parts of thing lactobacillus, 16~18 parts of enterococcus faecalis, 11~14 parts of bacillus subtilis, 6~8 parts of saccharomyces cerevisiae, fiber two 18~22 parts of 11~14 parts of sugared lactobacillus and complex enzyme formulation.
In the preferred embodiment of the present invention, above-mentioned leavening is mainly made up of the raw material of following weight portion：Plant 23 parts of thing lactobacillus, 18 parts of enterococcus faecalis, 13 parts of bacillus subtilis, 8 parts of saccharomyces cerevisiae, 13 parts of lactobacillus cellobiosas and multiple 20 parts of synthase preparation.
In the preferred embodiment of the present invention, above-mentioned complex enzyme formulation is protease, cellulase, xylan Two or more mixing composition in enzyme, mannonase galactosidase and glucose oxidase.
In above-mentioned preferred embodiment, above-mentioned complex enzyme formulation includes following components in percentage by weight：Protease 5~ 15%th, cellulase 20~30%, zytase 15~20%, mannase 15~20%, galactosidase 25~30% With glucose oxidase 5~10%.
In above-mentioned preferred embodiment, protease can be digested by the globulin in fermentation raw material and with globulin, Globulin and companion's globulin enzymatic hydrolyzation can reach more than 90%.
In the present invention, the above-mentioned typical but non-limiting content of protease is：5%th, 6%, 7%, 8%, 9%, 10%, 11%th, 12%, 13%, 14% or 15%.
In above-mentioned preferred embodiment, cellulase and zytase can significantly improve the water of protein in fermentation raw material Xie Du.Meanwhile, in cellulase and the present invention there is the synergy mutually promoted in fermented bacterium lactobacillus cellobiosas, can be with The enzymatic saccharification of cellulose and lactic fermentation are coupled together synchronously to be carried out, and eliminates product (glucose, cellobiose) to fibre The feedback inhibition of the plain enzyme of dimension, improves the enzymolysis efficiency of cellulose, while the lactic acid hair of accelerating fibers disaccharides lactobacillus The carrying out of ferment effect.
In the present invention, the above-mentioned typical but non-limiting content of cellulase is：20%th, 22%, 24%, 26%, 28% Or 30%.
In the present invention, the above-mentioned typical but non-limiting content of zytase is：15%th, 16%, 17%, 18%, 19% Or 20%.
In above-mentioned preferred embodiment, mannase and galactosidase can make stachyose and cottonseed in fermentation raw material The degradation efficiency of sugar can reach 90% and 70% respectively.
In the present invention, the above-mentioned typical but non-limiting content of mannase is：15%th, 16%, 17%, 18%, 19% or 20%.
In the present invention, the above-mentioned typical but non-limiting content of galactosidase is：25%th, 26%, 27%, 28%, 29% or 30%.
In above-mentioned preferred embodiment, glucose oxidase can effectively prevent the generation of mould miscellaneous bacteria, consume oxygen Gas, produces gluconic acid, promotes the formation of anaerobic environment, is conducive to the growth and breeding of lactic acid bacteria, promotes the carrying out of fermentation process, Ensure the quality of fermentation, extend shelf life of products.Meanwhile, glucose oxidase can be by glucose under conditions of the presence of oxygen Gluconic acid is converted into, the effect of checking that glucose expressed cellulase system can be released, improve cellulase system to cellulose The synergy efficiency of material.
In the present invention, the above-mentioned typical but non-limiting content of glucose oxidase is：5%th, 6%, 7%, 8%, 9% or 10%.
In the preferred embodiment of the present invention, above-mentioned protease by neutral proteinase and acid protease with 1：1 Weight than uniform mixing composition.
In above-mentioned preferred embodiment, the protease being made up of neutral proteinase and acid protease can be by proferment Globulin and companion's globulin in material are digested, and globulin and companion's globulin enzymatic hydrolyzation can reach more than 90%.
According to an aspect of the present invention, a kind of fermented feed, above-mentioned fermented feed is fermented by above-mentioned feed fermentation agent Arrive.
Fermented fodder nutrient composition of the present invention enriches, fragrant odour, and sour-sweet soft, moisture is high, and palatability is splendid, poultry Fowl feed intake is high, digests and assimilates, and the probiotics by fermentation in agent produces for protein in feed, fat and complex carbohydrates After the degradation of raw hydrolase, fermenting enzyme and respiratory enzyme, feed conversion rate is significantly improved, and also can provide dimension life The various bioactivators such as element, organic acid and somatomedin, accelerate growth of animals or poultry, improve livestock and poultry production performance, reduce material Meat (material egg) compares.
Meanwhile, fermented feed of the present invention is rich in substantial amounts of beneficial viable bacteria, is beneficial to microbial population of animal intestinal tract balance, with preventing and treating Livestock and poultry alimentary tract flora imbalance, significantly reduces grice diarrhoea and reduces the function of prevention of sow constipation, while animal machine can also be improved Body immunity function, produces various antibacterial materials, such as lactein, acidophilin, bacitracin and organic acid, hydrogen peroxide, Suppress the growth and breeding of pathogen, so that disease preventing and treating, reduces the use of antibiotic, animal drug residue free does not produce resistance Property, animal products quality safety, meat consolidation be very popular, can realize high quality and favourable price, and its economic benefit shows very much Write.
Additionally, the probiotics in fermented feed of the present invention is with livestock and poultry body, and original normal bacterium acts synergistically, animal is protected Intestinal health, suppresses the propagation of spoilage organisms in animal intestinal tract, so as to reduce the generation of harmful substance, reduces protein to ammonia and amine Conversion, reduce nitrosamine, ammonia, indoles, the generation of the harmful substance such as scatol, foul odour is eliminated, so as to reduce these things Matter has purified air in house by excrement pollution on the environment, and the growing environment of clean, sanitary pleasure is provided for livestock and poultry, It is also advantageous for cultivating the physically and mentally healthy of people simultaneously and is in a cheerful frame of mind.
According to an aspect of the present invention, the preparation method of above-mentioned fermented feed, the above method comprises the following steps：
(1) above-mentioned feed fermentation agent is put into fermentation raw material and is inoculated with and is well mixed, be subsequently added water by after inoculation The water content of fermentation raw material adjust to 30~50%, fermentation raw material to be fermented is obtained；
(2) above-mentioned steps (1) fermentation raw material to be fermented is fitted into one-way exhaust equipment and is fermented, at 30~40 DEG C Lower fermentation 3~7 days, obtains fermented feed.
In the preferred embodiment of the present invention, by the above-mentioned feed fermentation agent of above-mentioned steps (1) at 30~50 DEG C After the water dissolves of left and right, it is inoculated with to fermentation raw material.
It is above-mentioned preferred embodiment in, by feed fermentation agent after 30~50 DEG C or so of water dissolves, to fermentation Raw material is inoculated with, and can to a certain extent activate leavening, shortens the time of fermentation.
In the preferred embodiment of the present invention, above-mentioned steps (1) are obtained into fermentation raw material addition to be fermented to fit The calcium carbonate of amount adjusts pH value to 6.2 or so.
It is above-mentioned preferred embodiment in, the initial pH value that will ferment is reduced to 6.2 or so, during the fermentation can be with Some macromolecular substances (such as protein, polysaccharide, fat) are accelerated constantly to degrade so that the nutritive value of fermented feed of the present invention Obtain significantly being lifted very much.
In the present invention, above-mentioned fermentation raw material to be fermented is fermented in being fitted into one-way exhaust equipment, whole mistakes of fermentation Journey is carried out in one-way exhaust equipment.The gas that microorganism produces during the fermentation is arranged after reaching setting pressure by unidirectional Air valve is discharged, but the air in the external world can not enter in Zymolysis Equipment all the time.This unidirectional exhaust apparatus ensure that unidirectional row Anaerobic in gas equipment and the environment without living contaminants, it is ensured that the activity of microorganism, dexterously solve microbe in solid state hair The problems such as radiating, the anaerobism control of ferment, significantly reduce the production cost of fermentative feedstuff of microbe.
Fermentation process of the present invention, the contradiction flora for solving long-standing problem field of microbial fermentation coexists this worldwide difficulty Topic.The strain that the present invention is selected is have aerobic bacteria, and also including facultative anaerobic bacteria, under normal conditions, this two kinds of microorganisms are not It is compatible.But there is one-way exhaust equipment, this problem is solved very well.Aerobic bacteria such as saccharomyces cerevisiae in the present invention Bacterium can quickly consume oxygen, and be resistant to very strong sour environment, and (this process compares after aerobic bacteria runs out of oxygen It is short), a small amount of alcohol and lactic acid is generated, while the pH value of material has also declined, so that for lactobacillus plantarum is this kind of The growth and breeding of anaerobic bacteria provides good precondition.With the growth metabolism of Lactobacillus plantarum, the number of Lactobacillus plantarum Amount is continuously increased, and the organic acid of generation is also continuously increased, the pH value continuous decrease of material, some macromolecular substances (such as protein, Polysaccharide, fat etc.) constantly degraded, and the quantity of some organic acids, small peptide, free amino acid and vitamin is constantly increasing simultaneously Plus so that the nutritive value of fermented feed of the present invention has obtained significantly being lifted very much.
In the preferred embodiment of the present invention, above-mentioned fermentation raw material is dregs of beans, corn, wheat bran, rice bran meal, cassava The mixing of one or more of slag and corn germ cake.
It is above-mentioned preferred embodiment in, above-mentioned fermentation raw material is made up of dregs of beans and wheat bran, both dregs of beans and wheat bran Weight ratio is 4：1.
It is above-mentioned preferred embodiment in, above-mentioned fermentation raw material is made up of dregs of beans, corn and wheat bran, dregs of beans, corn and The weight ratio of three kinds of raw materials of wheat bran is 4：5：1.
It is above-mentioned preferred embodiment in, above-mentioned fermentation raw material is by wheat bran, rice bran meal, maize peel and corn germ cake group Into wheat bran, rice bran meal, the weight ratio of four kinds of raw materials of maize peel and corn germ cake are 1：2：1：3.
It is above-mentioned preferred embodiment in, above-mentioned fermentation raw material is by dregs of beans, corn, wheat bran, rice bran meal, manioc waste and jade Rice plumule dregs of rice composition, dregs of beans, corn, wheat bran, rice bran meal, the weight ratio of six kinds of raw materials of manioc waste and corn germ cake are 6：4：5： 5：5：5.
In the present invention, fermentation raw material cannot be only used for the tradition such as fermented bean dregs, corn, wheat bran, rice bran meal, corn germ cake Feedstuff, and can be used for the fermentation of the unconventional feed such as maize peel and manioc waste, improve the nutriture value of these feeds Value and animal palatability, so as to improve cultivation efficiency, improve the quality of meat products, reduce fermentation raw material cost.
In the preferred embodiment of the present invention, above-mentioned leavening and the weight ratio of fermentation raw material are 1~3：1000.
In the preferred embodiment of the present invention, above-mentioned one-way exhaust equipment is that respiratory membrane solid-state fermentation bag or band are single To the sealing container of air bleeding valve.
It is above-mentioned preferred embodiment in, fermentation raw material to be fermented is fitted into respiratory membrane solid-state fermentation bag and is sent out Ferment, all processes of fermentation are carried out in respiratory membrane solid-state fermentation bag.The gas that microorganism produces during the fermentation reaches and sets After constant-pressure by respiratory membrane discharge, but the air in the external world all the time can not enter respiratory membrane solid-state fermentation bag in (similar to A kind of check valve).This unidirectional exhaust apparatus ensure that the anaerobic in respiratory membrane solid-state fermentation bag and the ring without living contaminants Border, not only ensure that the activity of microorganism, while this first packaging, after fermentation are innovated technologies, dexterously solve microorganism The problems such as radiating, the anaerobism control of solid state fermentation, significantly reduce the production cost of fermentative feedstuff of microbe.
Embodiment 1
A kind of feed fermentation agent, above-mentioned leavening is mainly made up of the raw material of following weight portion：20 parts of Lactobacillus plantarum, excrement 15 parts of 15 parts of enterococcus, 10 parts of bacillus subtilis, 5 parts of saccharomyces cerevisiae, 10 parts of lactobacillus cellobiosas and complex enzyme formulation, its In,
Above-mentioned complex enzyme formulation is by following percentage by weight into being grouped into：Protease 15%, cellulase 20%, wood are poly- Carbohydrase 15%, mannase 15%, galactosidase 25% and glucose oxidase 10%.
Embodiment 2
A kind of feed fermentation agent, above-mentioned leavening is mainly made up of the raw material of following weight portion：25 parts of Lactobacillus plantarum, excrement 25 parts of 20 parts of enterococcus, 15 parts of bacillus subtilis, 10 parts of saccharomyces cerevisiae, 15 parts of lactobacillus cellobiosas and complex enzyme formulation, Wherein,
Above-mentioned complex enzyme formulation is by following percentage by weight into being grouped into：Protease 50%, cellulase 50%.
Embodiment 3
A kind of feed fermentation agent, above-mentioned leavening is mainly made up of the raw material of following weight portion：24 parts of Lactobacillus plantarum, excrement 22 parts of 16 parts of enterococcus, 11 parts of bacillus subtilis, 7 parts of saccharomyces cerevisiae, 12 parts of lactobacillus cellobiosas and complex enzyme formulation, its In,
Above-mentioned complex enzyme formulation is by following percentage by weight into being grouped into：Protease 15%, cellulase 20%, wood are poly- Carbohydrase 25%, galactosidase 25% and glucose oxidase 20%.
Embodiment 4
A kind of feed fermentation agent, above-mentioned leavening is mainly made up of the raw material of following weight portion：21 parts of Lactobacillus plantarum, excrement 16 parts of 19 parts of enterococcus, 14 parts of bacillus subtilis, 6 parts of saccharomyces cerevisiae, 13 parts of lactobacillus cellobiosas and complex enzyme formulation, its In,
Above-mentioned complex enzyme formulation is by following percentage by weight into being grouped into：Protease 15%, cellulase 20%, wood are poly- Carbohydrase 15%, mannase 15%, galactosidase 25% and glucose oxidase 10%.
Embodiment 5
A kind of feed fermentation agent, above-mentioned leavening is mainly made up of the raw material of following weight portion：23 parts of Lactobacillus plantarum, excrement 20 parts of 18 parts of enterococcus, 13 parts of bacillus subtilis, 8 parts of saccharomyces cerevisiae, 12 parts of lactobacillus cellobiosas and complex enzyme formulation, its In,
Above-mentioned complex enzyme formulation is by following percentage by weight into being grouped into：Protease 15%, cellulase 20%, wood are poly- Carbohydrase 15%, mannase 15%, galactosidase 25% and glucose oxidase 10%.
Embodiment 6
A kind of preparation method of fermented feed, the above method comprises the following steps：
(1) fermentation raw material is prepared：Uniformly mixing is constituted the main raw material by following weight portion of above-mentioned fermentation raw material：Dregs of beans 4 1 part of part and wheat bran；
(2) it is inoculated with：By the feed fermentation agent of above-described embodiment 1 with 30 DEG C of water dissolves, above-mentioned steps (1) hair is then placed in It is inoculated with ferment raw material, is subsequently added water and is well mixed, the water content for adjusting postvaccinal fermentation raw material is 30%, after inoculation is obtained Fermentation raw material；
(3) fermented：Above-mentioned postvaccinal fermentation raw material is fitted into one-way exhaust equipment and is fermented, at 30 DEG C Fermentation 3 days, obtains fermented feed.
Embodiment 7
A kind of preparation method of fermented feed, the above method comprises the following steps：
(1) fermentation raw material is prepared：Uniformly mixing is constituted the main raw material by following weight portion of above-mentioned fermentation raw material：Dregs of beans 4 Part, 5 parts of corn, 1 part of wheat bran；
(2) it is inoculated with：By the feed fermentation agent of above-described embodiment 2 with 50 DEG C of water dissolves, above-mentioned steps (1) hair is then placed in It is inoculated with ferment raw material, is subsequently added water and is well mixed, the water content for adjusting postvaccinal fermentation raw material is 50%, after inoculation is obtained Fermentation raw material；
(3) fermented：Above-mentioned postvaccinal fermentation raw material is fitted into one-way exhaust equipment and is fermented, at 40 DEG C Fermentation 7 days, obtains fermented feed.
Embodiment 8
A kind of preparation method of fermented feed, the above method comprises the following steps：
(1) fermentation raw material is prepared：Uniformly mixing is constituted the main raw material by following weight portion of above-mentioned fermentation raw material：Dregs of beans 6 Part, 4 parts of corn, 5 parts of rice bran meal, 5 parts of wheat bran, 5 parts of manioc waste and 5 parts of corn germ cake；
(2) it is inoculated with：By the feed fermentation agent of above-described embodiment 3 with 40 DEG C of water dissolves, above-mentioned steps (1) hair is then placed in It is inoculated with ferment raw material, is subsequently added water and is well mixed, the water content for adjusting postvaccinal fermentation raw material is 38%, after inoculation is obtained Fermentation raw material；
(3) fermented：Above-mentioned postvaccinal fermentation raw material is fitted into one-way exhaust equipment and is fermented, at 32 DEG C Fermentation 4 days, obtains fermented feed.
Embodiment 9
A kind of preparation method of fermented feed, the above method comprises the following steps：
(1) fermentation raw material is prepared：Uniformly mixing is constituted the main raw material by following weight portion of above-mentioned fermentation raw material：Wheat bran 1 Part, 2 parts of rice bran meal, 1 part of maize peel and 3 parts of corn germ cake；
(2) it is inoculated with：By the feed fermentation agent of above-described embodiment 4 with 50 DEG C of water dissolves, above-mentioned steps (1) hair is then placed in It is inoculated with ferment raw material, is subsequently added water and is well mixed, the water content for adjusting postvaccinal fermentation raw material is 45%, after inoculation is obtained Fermentation raw material；
(3) fermented：Above-mentioned postvaccinal fermentation raw material is fitted into one-way exhaust equipment and is fermented, at 34 DEG C Fermentation 6 days, obtains fermented feed.
Embodiment 10
A kind of preparation method of fermented feed, the above method comprises the following steps：
(1) fermentation raw material is prepared：Uniformly mixing is constituted the main raw material by following weight portion of above-mentioned fermentation raw material：Dregs of beans 10 Part, 10 parts of corn, 10 parts of wheat bran, 8 parts of rice bran meal, 8 parts of manioc waste, 5 parts of maize peel and 5 parts of corn germ cake；
(2) it is inoculated with：By the feed fermentation agent of above-described embodiment 5 with 50 DEG C of water dissolves, above-mentioned steps (1) hair is then placed in It is inoculated with ferment raw material, is subsequently added water and is well mixed, the water content for adjusting postvaccinal fermentation raw material is 40%, after inoculation is obtained Fermentation raw material；
(3) fermented：Above-mentioned postvaccinal fermentation raw material is fitted into one-way exhaust equipment and is fermented, at 35 DEG C Fermentation 7 days, obtains fermented feed.
Comparative example 1
A kind of feed fermentation agent, above-mentioned leavening is mainly made up of the raw material of following weight portion：23 parts of Lactobacillus plantarum, excrement 12 parts of 18 parts of enterococcus, 13 parts of bacillus subtilis, 8 parts of saccharomyces cerevisiae and lactobacillus cellobiosas.
Comparative example 2
A kind of feed fermentation agent, above-mentioned leavening is mainly made up of the raw material of following weight portion：23 parts of Lactobacillus plantarum, excrement 20 parts of 18 parts of enterococcus, 13 parts of bacillus subtilis, 8 parts of saccharomyces cerevisiae, 12 parts of lactobacillus cellobiosas and complex enzyme formulation, its In,
Above-mentioned complex enzyme formulation is by following percentage by weight into being grouped into：Protease 25%, mannase 25%, half Lactoside enzyme 25% and glucose oxidase 25%.
Comparative example 3
A kind of feed fermentation agent, above-mentioned leavening is mainly made up of the raw material of following weight portion：23 parts of Lactobacillus plantarum, excrement 20 parts of 18 parts of enterococcus, 13 parts of bacillus subtilis, 8 parts of saccharomyces cerevisiae, 12 parts of lactobacillus cellobiosas and complex enzyme formulation, its In,
Above-mentioned complex enzyme formulation is by following percentage by weight into being grouped into：It is cellulase 20%, zytase 40%, sweet Dew dextranase 15%, galactosidase 25%.
Comparative example 4
A kind of feed fermentation agent, above-mentioned leavening is mainly made up of the raw material of following weight portion：23 parts of Lactobacillus plantarum, excrement 20 parts of 18 parts of enterococcus, 13 parts of bacillus subtilis, 8 parts of saccharomyces cerevisiae, 12 parts of lactobacillus cellobiosas and complex enzyme formulation, its In,
Above-mentioned complex enzyme formulation is by following percentage by weight into being grouped into：Protease 25%, cellulase 30%, wood are poly- Carbohydrase 15% and glucose oxidase 40%.
Comparative example 5
A kind of preparation method of fermented feed, the above method comprises the following steps：
(1) fermentation raw material is prepared：Uniformly mixing is constituted the main raw material by following weight portion of above-mentioned fermentation raw material：Dregs of beans 10 Part, 10 parts of corn, 10 parts of wheat bran, 8 parts of rice bran meal, 8 parts of manioc waste, 5 parts of maize peel and 5 parts of corn germ cake；
(2) it is inoculated with：By the feed fermentation agent of above-mentioned comparative example 1 with 50 DEG C of water dissolves, above-mentioned steps (1) hair is then placed in It is inoculated with ferment raw material, is subsequently added water and is well mixed, the water content for adjusting postvaccinal fermentation raw material is 40%, after inoculation is obtained Fermentation raw material；
(3) fermented：Above-mentioned postvaccinal fermentation raw material is fitted into one-way exhaust equipment and is fermented, at 35 DEG C Fermentation 7 days, obtains fermented feed.
Comparative example 6
A kind of preparation method of fermented feed, the above method comprises the following steps：
(1) fermentation raw material is prepared：Uniformly mixing is constituted the main raw material by following weight portion of above-mentioned fermentation raw material：Dregs of beans 10 Part, 10 parts of corn, 10 parts of wheat bran, 8 parts of rice bran meal, 8 parts of manioc waste, 5 parts of maize peel and 5 parts of corn germ cake；
(2) it is inoculated with：By the feed fermentation agent of above-mentioned comparative example 2 with 50 DEG C of water dissolves, above-mentioned steps (1) hair is then placed in It is inoculated with ferment raw material, is subsequently added water and is well mixed, the water content for adjusting postvaccinal fermentation raw material is 40%, after inoculation is obtained Fermentation raw material；
(3) fermented：Above-mentioned postvaccinal fermentation raw material is fitted into one-way exhaust equipment and is fermented, at 35 DEG C Fermentation 7 days, obtains fermented feed.
Comparative example 7
A kind of preparation method of fermented feed, the above method comprises the following steps：
(1) fermentation raw material is prepared：Uniformly mixing is constituted the main raw material by following weight portion of above-mentioned fermentation raw material：Dregs of beans 10 Part, 10 parts of corn, 10 parts of wheat bran, 8 parts of rice bran meal, 8 parts of manioc waste, 5 parts of maize peel and 5 parts of corn germ cake；
(2) it is inoculated with：By the feed fermentation agent of above-mentioned comparative example 3 with 50 DEG C of water dissolves, above-mentioned steps (1) hair is then placed in It is inoculated with ferment raw material, is subsequently added water and is well mixed, the water content for adjusting postvaccinal fermentation raw material is 40%, after inoculation is obtained Fermentation raw material；
(3) fermented：Above-mentioned postvaccinal fermentation raw material is fitted into one-way exhaust equipment and is fermented, at 35 DEG C Fermentation 7 days, obtains fermented feed.
Comparative example 8
A kind of preparation method of fermented feed, the above method comprises the following steps：
(1) fermentation raw material is prepared：Uniformly mixing is constituted the main raw material by following weight portion of above-mentioned fermentation raw material：Dregs of beans 10 Part, 10 parts of corn, 10 parts of wheat bran, 8 parts of rice bran meal, 8 parts of manioc waste, 5 parts of maize peel and 5 parts of corn germ cake；
(2) it is inoculated with：By the feed fermentation agent of above-mentioned comparative example 4 with 50 DEG C of water dissolves, above-mentioned steps (1) hair is then placed in It is inoculated with ferment raw material, is subsequently added water and is well mixed, the water content for adjusting postvaccinal fermentation raw material is 40%, after inoculation is obtained Fermentation raw material；
(3) fermented：Above-mentioned postvaccinal fermentation raw material is fitted into one-way exhaust equipment and is fermented, at 35 DEG C Fermentation 7 days, obtains fermented feed.
Effect example 1
For show to add in fermented feed agent of the present invention complex enzyme formulation can as the supplement of strain extracellular proteinase, plus The startup of fast fermentation and efficiency, shorten the total time needed for completing fermentation, improve the quality of fermented product, improve fermentation efficiency and Benefit.Fermented feed agent prepared by existing special Example 10 and comparative example 5~8 carries out fermentation contrast test, and right respectively The bacteria colony count of every gram of organoleptic indicator, the bacteria colony count (cfu/g) of every gram of lactic acid bacteria rhzomorph and Escherichia coli rhzomorph is carried out Statistics, it is as a result as shown in the table：
As seen from the above table, after the embodiment of the present invention 10 is fermented 7 days at 35 DEG C, fermented feed is obtained, coliform count is only For 5cfu/g, lactic acid bacteria number are 1,000,000,000 cfu/g, outward appearance is light yellow, and without the peculiar smell that goes mouldy, smell acid is fragrant, and quality moistening is soft, structure Loosely, ferment very abundant.And comparative example 5 is not by including the feed and comparative example 6 of the fermented feed agent preparation of complex enzyme formulation ~8 feeds that only prepared by the fermented feed agent comprising part complex enzyme formulation, after being fermented 7 days at 35 DEG C, feed still keeps Primary colors, tasteless, fibre structure are coarse, moisture is big, partly cohere agglomerating, it is known that fermentation is not fully abundant.
It follows that add complex enzyme formulation can as the supplement of strain extracellular proteinase, accelerate fermentation startup with Efficiency, shortens the total time needed for completing fermentation, improves the quality of fermented product, improves fermentation efficiency and benefit.
Effect example 2
To show to be rich in substantial amounts of beneficial viable bacteria in fermented feed of the present invention, it is beneficial to microbial population of animal intestinal tract balance, has Preventing and treating livestock and poultry alimentary tract flora imbalance, improves animal body immunologic function.Existing spy pick out three kinds of ages in days it is close be respectively 97 The sick pig with intestinal tract disease of age in days or so 6,137 ages in days or so 8,156 ages in days or so 8, wherein,
97 age in days test groups 3, initial population weight is 105.3Kg, and the equal body weight of head is 35.1Kg, and control group 3 is initial total Body weight is 122.1Kg, and the equal body weight of head is 40.7Kg；
137 age in days test groups 4, initial population weight is 238Kg, and the equal body weight of head is 59.5Kg, and control group 4 is initial total Body weight is 246Kg, and the equal body weight of head is 61.5Kg；
146 age in days test groups 4, initial population weight be 249.2Kg, the equal body weight of head be 62.3Kg, control group 4, initially TBW is 266.8Kg, and the equal body weight of head is 66.7Kg.
Feeding method：
Control group fed basal diet, test group is prepared in basal diet in the 10% ratio addition embodiment of the present invention 10 Fermented feed.The raising (addition fermented feed 5%) of 5 days prerun transitional periods, prerun transitional period knot were first carried out before on-test Shu Hou, is then transferred to the formal phase (addition fermented feed is 10%), and experimental period is 32 days.Two groups of feeding manners are used freely to be adopted Food and drinking technique, other additives, daily feeding 2 times such as antibiotic are not added additionally for examination feed.Pig farm program is pressed in epidemic prevention It is unified to carry out.Daily morning sweeps colony house health, the health status of every day entry daily ingestion amount and pig.Respectively in examination during experiment When testing beginning and off-test, record of weighing is carried out to pig respectively, and calculate the data such as daily gain, feed intake and feedstuff-meat ratio.
Effect assessment
Note of weighing is carried out to pig respectively in on-test and off-test respectively during testing the effect above example 2 Record, and it is as shown in the table to calculate the data such as daily gain, feed intake and feedstuff-meat ratio：
As seen from the above table, the test group of the fermented feed for being prepared using the embodiment of the present invention 10 is better than control group；In experiment After end, the contrast control group average daily gain of 97 age in days test groups improves 41.6%, and feedstuff-meat ratio is relative to control group reduction 21.9%；The contrast control group average daily gain of 137 age in days test groups improves 28.11%, and feedstuff-meat ratio is relative to control group reduction 14.7%；The contrast control group average daily gain of 146 age in days test groups improves 29.17%, and feedstuff-meat ratio is relative to control group reduction 14.3%.Difference reaches the level of signifiance (P ＜ 0.05), using fermented feed sick pig can be promoted to get well in this explanation daily ration And feed intake, it is beneficial to microbial population of animal intestinal tract balance, livestock and poultry alimentary tract flora imbalance can be prevented and treated, improve animal body and be immunized Power, remarkably promotes pig growth, improves daily gain, improves feed efficiency.
Overall economic efficiency analysis is carried out to the effect above example 2, to test pig weightening, feed consumption rate, feed cost and income Statistics is analyzed, it is as a result as shown in the table：
As seen from the above table, in terms of 19.8 yuan/Kg of the live pig pork wholesale price of on December 6th, 2016, feed valency during experiment Lattice are 2.75 yuan/Kg after being respectively test group addition fermented feed, and control group is 2.62 yuan/Kg, calculates test group and control group Economic benefit is during experiment：97 450.4 yuan/heads of age in days test group, 279.7 yuan/head of control group, test group is improved compared with control group 61.1%；137 432.2 yuan/heads of age in days test group, 309.4 yuan/head of control group, test group relatively improves 39.7% according to group；146 days 482.8 yuan/head of age test group, 303.1 yuan/head of control group, test group improves 59.3% compared with control group.
Additionally, being found in being tested by effect example 2, the feeding of test group when feeding fermented feed of the present invention is added in daily ration Situation is very vigorous, hence it is evident that love eats fermented feed, and the feed intake of test group starts substantially increase after one week.Feeding fermented feed two Sick pig feed intake returns to the grazing rate more than 80% of normal development after week, state of being got well substantially after 26 days, overall disease-resistant Ability strengthens, and the dispensing ratio that reduces medicine is, the mixed economy interests for significantly improving pig farm.
Effect example 3
To show that fermented fodder nutrient of the present invention enriches, fragrant odour, sour-sweet soft, moisture is high, and palatability is splendid, Live pig feeding amount is high, digests and assimilates, with accelerate pig growth, improve production performance, reduce feedstuff-meat ratio the characteristics of.Now select 800 miscellaneous pork pigs of DLY ternary of 56~85 close ages in days of body weight, are randomly divided into 2 groups, i.e. test group and control group, every group 400, sex ratio is consistent, and being weighed through inspection, it is consistent (P ＞ 0.05) to reach homogeneity.
Feeding method：
The common complete diet pellet of control group fed, test group adds the embodiment of the present invention 10 in common complete diet pellet in 10% ratio The fermented feed of preparation, antibiotic or other additives are not added additionally for examination feed.Prerun was first carried out before on-test The raising (adding proportion 3%) of 7 days phases is crossed, at the end of the prerun transitional period, test pig keeps group difference not significantly (P ＞ 0.05) the positive examination phase (fermented feed adding proportion is 10%), is then transferred to, 102 days experimental periods are until deliver for sale.Daily feeding 2 times, Two groups of feeding manners use free choice feeding, and free water, epidemic prevention is carried out by this pig farm program unification.Respectively in examination during experiment Testing initial stage and latter stage carries out record of weighing to pig, calculates daily gain, feed intake, feedstuff-meat ratio.
Effect assessment
Note of weighing is carried out to pig respectively in on-test and off-test respectively during testing the effect above example 3 Record, and it is as shown in the table to calculate the data such as daily gain, feed intake and feedstuff-meat ratio：
As seen from the above table, test group whole process average daily gain is 918.9g/ heads, and control group whole process average daily gain is 857.4g/ heads, test group improves 7.2% than control group；Test group whole process feedstuff-meat ratio is 2.5, the whole material meat of control group Than being 2.7, examination experimental group reduces 7.4% than control group.Difference reaches the level of signifiance (P ＜ 0.05), in this explanation daily ration Pig can be remarkably promoted using fermented feed to grow, improve daily gain, reduce feedstuff-meat ratio, improve feed performance.
Overall economic efficiency analysis is carried out to the effect above example 3, to test pig weightening, feed consumption rate, feed cost and income Statistics is analyzed, it is as a result as shown in the table：
As seen from the above table, in terms of 14.6 yuan/Kg of live pig pork wholesale price, feed price is respectively test group during experiment It is 2.85 yuan/Kg after addition fermented feed, control group is 3.06 yuan/Kg, economic effect during calculating test group and control group experiment Benefit is：708.6 yuan/head of test group, 555.8 yuan/head of control group, test group improves 27.5% compared with control group.With medium-sized pig farm Live pig is delivered year for sale by 10000 calculating, using that can make feedstuff-meat ratio reduction by 0.2 after fermented feed prepared by the present invention, by every pig Average 120 kg delivers calculating for sale, and feedstuff-meat ratio reduction by 0.2 can save 24.0 kgs/head of feed.10000 amount to saving feed 24.0 ten thousand kilograms, feed price can make more than 720,000 yuan of pig farm annual increase income benefit based on 3 yuan of per kilogram.
From the experimental result of the effect above example 3, feeding fermented feed of the present invention is added in daily ration, can turn feed Rate strengthens, feedstuff-meat ratio reduction, and the speed of growth is accelerated, the rate of animals delivered to the slaughter-house shifts to an earlier date, hence it is evident that improve the mixed economy interests on pig farm.
Effect example 4
To show that fermented fodder nutrient of the present invention enriches, fragrant odour, sour-sweet soft, moisture is high, and palatability is splendid, Poultry feeding amount is high, digests and assimilates, with accelerate bird, improve birds production performance, reduce feedstuff-egg ratio the characteristics of.It is existing The blue plumage of laying hen 3064 in sea, 1980 plumages and 1720 plumages in the close early stage of laying eggs of age in days, mid-term and later stage are selected, is randomly divided into respectively 2 groups, i.e. test group and control group, every group of plumage of difference 1532,990 plumages and 860 plumages.
Test method：
Control group fed basal diet, test group is prepared in basal diet in the 5% ratio addition embodiment of the present invention 10 Fermented feed.The raising of 3 days prerun transitional periods was first carried out before on-test, at the end of the prerun transitional period, laying hen keeps for experiment Group difference significantly and then is not transferred to the positive examination phase, and the positive examination phase is 28 days.Pre-stage test group daily ration transitional period addition fermentation of laying eggs is raised Material 3%, experimental period 5%；Lay eggs the interim study group transitional period addition fermented feed 3%, experimental period 5%；Lay eggs later stage test group Transitional period adds fermented feed 3%, experimental period 5%.Antibiotic or other additives are not added additionally for examination feed, laying hen is certainly By searching for food, free water, epidemic prevention is carried out by plant's program unification.During experiment respectively experiment the initial stage, latter stage to laying hen with Machine is extracted and carries out record of weighing, and laying rate calculating, egg total amount/piece, day egg size amount/part, day egg gross weight/jin, daily ingestion amount/ Only, day total feed intake, day feedstuff-egg ratio carry out unified calculation priming.
Effect assessment
During testing the effect above example 4 respectively at the experiment initial stage, latter stage is randomly selected to laying hen carries out record of weighing, product The calculating of egg rate, egg total amount/piece, day egg gross weight/jin, daily ingestion amount/only, the data such as following table such as day total feed intake, day feedstuff-egg ratio It is shown：
As seen from the above table, fermented feed of the present invention is applied in the cultivation of laying hen, can make the reduction of laying hen feed intake, laying rate Increase, egg size is improved, substantially reduce feedstuff-egg ratio, strengthens the resistance against diseases of laying hen.Additionally, by the observation to eggshell, experimental group Laying hen production egg, its eggshell is smooth, and egg nutritional profile is obviously improved, it is seen that feed of the present invention have improve eggshell quality, Reduce shell-breaking rate, increase egg qualification rate, prevent substantially husky shell egg appearance advantage.
In summary, the fermented fodder nutrient composition that the present invention is provided enriches, and fragrant odour is sour-sweet soft, moisture Height, palatability is splendid, and livestock and poultry feed intake is high, digests and assimilates, and protein, fat and complex carbohydrates are by hair in feed After the degradation of hydrolase, fermenting enzyme and respiratory enzyme that the probiotics in ferment agent produces, feed conversion rate is significantly improved, and The various bioactivators such as vitamin, organic acid and somatomedin can also be provided, accelerate growth of animals or poultry, improve livestock and poultry life Performance is produced, material meat (material egg) ratio is reduced.
Meanwhile, fermented feed of the present invention is rich in substantial amounts of beneficial viable bacteria, is beneficial to microbial population of animal intestinal tract balance, with preventing and treating Livestock and poultry alimentary tract flora imbalance, significantly reduces grice diarrhoea and reduces the function of prevention of sow constipation, while animal machine can also be improved Body immunity function, produces various antibacterial materials, such as lactein, acidophilin, bacitracin and organic acid, hydrogen peroxide, Suppress the growth and breeding of pathogen, so that disease preventing and treating, reduces the use of antibiotic, animal drug residue free does not produce resistance Property, animal products quality safety, meat consolidation be very popular, can realize high quality and favourable price, and its economic benefit shows very much Write.
Additionally, the probiotics in fermented feed of the present invention is with livestock and poultry body, and original normal bacterium acts synergistically, animal is protected Intestinal health, suppresses the propagation of spoilage organisms in animal intestinal tract, so as to reduce the generation of harmful substance, reduces protein to ammonia and amine Conversion, reduce nitrosamine, ammonia, indoles, the generation of the harmful substance such as scatol, foul odour is eliminated, so as to reduce these things Matter has purified air in house by excrement pollution on the environment, and the growing environment of clean, sanitary pleasure is provided for livestock and poultry, It is also advantageous for cultivating the physically and mentally healthy of people simultaneously and is in a cheerful frame of mind.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent Pipe has been described in detail with reference to foregoing embodiments to the present invention, it will be understood by those within the art that：Its according to The technical scheme described in foregoing embodiments can so be modified, or which part or all technical characteristic are entered Row equivalent；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology The scope of scheme.

Claims (10)

1. a kind of feed fermentation agent, it is characterised in that the leavening is mainly made up of the raw material of following weight portion：Plant breast bar 20~25 parts of bacterium, 15~20 parts of enterococcus faecalis, 10~15 parts of bacillus subtilis, 5~10 parts of saccharomyces cerevisiae, cellobiose breast bar 15~25 parts of 10~15 parts of bacterium and complex enzyme formulation.
2. feed fermentation agent according to claim 1, it is characterised in that the main original by following weight portion of the leavening Material composition：21~24 parts of Lactobacillus plantarum, 16~18 parts of enterococcus faecalis, 11~14 parts of bacillus subtilis, saccharomyces cerevisiae 6~8 18~22 parts of part, 11~14 parts of lactobacillus cellobiosas and complex enzyme formulation.
3. feed fermentation agent according to claim 1, it is characterised in that the main original by following weight portion of the leavening Material composition：23 parts of Lactobacillus plantarum, 17 parts of enterococcus faecalis, 13 parts of bacillus subtilis, 8 parts of saccharomyces cerevisiae, cellobiose breast bar 20 parts of 13 parts of bacterium and complex enzyme formulation.
4. the feed fermentation agent according to any one of claims 1 to 3, it is characterised in that the complex enzyme formulation is albumen Two or more in enzyme, cellulase, zytase, mannonase galactosidase and glucose oxidase is mixed It is combined into；
Preferably, the complex enzyme formulation includes following components in percentage by weight：Protease 5~15%, cellulase 20~ 30%th, zytase 15~20%, mannase 15~20%, galactosidase 25~30% and glucose oxidase 5~ 10%.
5. feed fermentation agent according to claim 4, it is characterised in that the protease is neutral proteinase and acid egg White enzyme is with 1：1 weight ratio is uniformly mixed.
6. a kind of fermented feed, it is characterised in that the main feed as described in any one of Claims 1 to 5 of the fermented feed It is ferment-fermented to obtain.
7. a kind of preparation method of fermented feed according to claim 6, it is characterised in that methods described includes following step Suddenly：
(1) the feed fermentation agent described in any one of Claims 1 to 5 is put into fermentation raw material and is inoculated with and is well mixed, then Add water to adjust to 30~50% the water content of postvaccinal fermentation raw material, fermentation raw material to be fermented is obtained；
(2) step (1) fermentation raw material to be fermented is fitted into one-way exhaust equipment and is fermented, issued at 30~40 DEG C Ferment 3~7 days, obtains fermented feed.
8. the preparation method of fermented feed according to claim 7, it is characterised in that fermentation raw material is in the step (1) The mixing of one or more of dregs of beans, corn, wheat bran, rice bran meal, manioc waste, maize peel and corn germ cake；
Preferably, the fermentation raw material is made up of dregs of beans and wheat bran, and the weight ratio of both dregs of beans and wheat bran is 4：1；
Preferably, the fermentation raw material is made up of dregs of beans, corn and wheat bran, dregs of beans, the weight ratio of three kinds of raw materials of corn and wheat bran It is 4：5：1；
Preferably, the fermentation raw material is made up of wheat bran, rice bran meal, maize peel and corn germ cake, wheat bran, rice bran meal, corn The weight ratio of four kinds of raw materials of skin and corn germ cake is 1：2：1：3；
Preferably, the fermentation raw material is made up of dregs of beans, corn, wheat bran, rice bran meal, manioc waste and corn germ cake, dregs of beans, jade Rice, wheat bran, rice bran meal, the weight ratio of six kinds of raw materials of manioc waste and corn germ cake are 6：4：5：5：5：5.
9. the preparation method of fermented feed according to claim 7, it is characterised in that the step (1) feed fermentation agent is put It is 1~3 to enter the weight ratio being inoculated with fermentation raw material：1000.
10. the preparation method of fermented feed according to claim 7, it is characterised in that the one-way exhaust equipment is breathing Film solid-state fermentation bag or the sealing container with one-way exhaust valve.
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