CN109043123A - The preparation method and application of fermented feed, fermented feed - Google Patents
The preparation method and application of fermented feed, fermented feed Download PDFInfo
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- CN109043123A CN109043123A CN201811246213.7A CN201811246213A CN109043123A CN 109043123 A CN109043123 A CN 109043123A CN 201811246213 A CN201811246213 A CN 201811246213A CN 109043123 A CN109043123 A CN 109043123A
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- fermented feed
- bacterium
- lactobacillus
- dregs
- homofermentative lactic
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- 238000002360 preparation method Methods 0.000 title claims abstract description 38
- 241000894006 Bacteria Species 0.000 claims abstract description 166
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 164
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 82
- 239000004310 lactic acid Substances 0.000 claims abstract description 82
- 238000000855 fermentation Methods 0.000 claims abstract description 57
- 230000004151 fermentation Effects 0.000 claims abstract description 48
- 108091005804 Peptidases Proteins 0.000 claims abstract description 43
- 239000004365 Protease Substances 0.000 claims abstract description 43
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 43
- 241000186869 Lactobacillus salivarius Species 0.000 claims abstract description 38
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- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 37
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- 238000011081 inoculation Methods 0.000 claims abstract description 7
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- 241001071864 Lethrinus laticaudis Species 0.000 description 2
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 2
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- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
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- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/137—Delbrueckii
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/181—Salivarius
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/231—Lactis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/41—Pediococcus
- A23V2400/413—Acidilactici
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Botany (AREA)
- Birds (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Fodder In General (AREA)
Abstract
The invention belongs to field of feed, it is related to the preparation method and application of a kind of fermented feed, fermented feed.Lactobacillus inoculation used in fermented feed of the invention includes homofermentative lactic bacterium and protease；The homofermentative lactic bacterium bag includes at least two in lactobacillus acidophilus, Lactobacillus salivarius, Lactobacillus delbrueckii, Pediococcus acidilactici, lactobacillus thermophilus, enterococcus faecalis and Lactococcus lactis.The present invention is combined using homofermentative lactic bacterium and protease for the first time ferments, and substantially increases lactic acid content, and lactic acid conversion ratio is high, so that the fermented feed becomes high-content lactic fermentation feed；Have the characteristics that green, efficient, safe and pollution-free, palatability is good, easy to digest, for improving the microecological balance of animal stomach, it is highly beneficial to improve animal health.
Description
Technical field
The invention belongs to field of feed, in particular to a kind of fermented feed, fermented feed preparation method and
Using.
Background technique
Fermented feed, using plant by-product as primary raw material, is made by the metabolism of beneficial bacterium under artificial control condition
With degradation part macro-molecular protein, fat and polysaccharide etc. generate the small molecules nutrition such as active small peptide amino acid, organic acid
The mix products of substance and beneficial bacterium.There is fermented feed improvement feed palatability, raising feed nutritive value, nutrition more to close
Reason, lower production costs and the characteristics of make full use of a variety of feedstuffs, research and development have a high potential, and are that current safe nonreactive is strong
The important directions of health feed advance.The pH value of fermented feed is generally 4.5 hereinafter, contain the organic acids, lactic acid such as a large amount of lactic acid
Bacterium and other beneficial microbes, and the harmful microorganism in fermented feed can be pressed down by acidic environment and other antibacterial substances
System.
Lactic acid is a kind of compound, and molecular formula is C3H6O3；Its carboxyl releases a proton in aqueous solution, and produces
Lactogenesis acid ion.Lactic acid is by lactobacillus-fermented come the raw material of fermentation method is usually starchy material in fermented feed.Cream
There are many lactic acid bacteria that the acid fermentation stage can produce acid, but it is higher few to produce acid amount.Its fermentation approach of different fungus strains is different,
It generally can be divided into homofermentation, heterofermentation and facultative fermentation.The lactic acid that fermentation generates has good palatability, can inhibit intestines
Road harmful bacteria improves intestinal environment and forms dominant microflora, and Competitive assays harmful bacteria improves immunity of organisms.Lactic acid is in animal body
The interior flourish that the low pH of enteron aisle can be maintained to be conducive to probiotics, Feed Energy of the feeding containing lactic acid improve animal intestinal tract health and life
Long performance.For example, the piggy of nursing period can catch by Escherichia coli and salmonellal disease, lactic acid is added in feed
It can prevent growth of pathogenic bacteria in piggy lower gastrointestinal tract.Wang et al. (2015) research confirms that lactic acid can inhibit the conjunction of bacterio protein
At finding that bacterium will lead to disintegrating for microbial cell film and structure intracellular through lactic acid treatment through micro- sem observation.In addition, newborn
Acid is also widely used in food, can extend the shelf life, or even control microbial contamination.
Currently, the applicable cases of biological fermentation feed are unsatisfactory, application effect is not good enough, is mainly caused by following reason
: (1) strain fall behind, and lactobacillus inoculation selection is bad；(2) zymotechnique is not complete (not good enough).The current fermented feed containing lactic acid
Many is all the method using anaerobic fermentation and acid production, but still has the disadvantage in that lactic acid content is low, is not higher than 10%；Lactic acid turns
It changes inefficient；Fermentation time is long, and energy consumption is high, high production cost.
In consideration of it, the present invention is specifically proposed.
Summary of the invention
The first object of the present invention is to provide a kind of fermented feed, to overcome the above problem or at least be partially solved
Above-mentioned technical problem.
The second object of the present invention is to provide a kind of preparation method of fermented feed, and simple process is easy to implement, can
Overcome the above problem or at least is partially solved above-mentioned technical problem.
The third object of the present invention is to provide fermented feed described in one kind or the preparation using the fermented feed
The fermented feed that method obtains is preparing the application in pannage.
To achieve the above object, the technical solution adopted by the present invention are as follows:
According to an aspect of the present invention, the present invention provides a kind of fermented feed, lactic acid bacteria used in the fermented feed
Kind includes homofermentative lactic bacterium and protease；
The homofermentative lactic bacterium bag includes lactobacillus acidophilus, Lactobacillus salivarius, Lactobacillus delbrueckii, Pediococcus acidilactici, thermophilic
At least two in hot lactobacillus, enterococcus faecalis and Lactococcus lactis.
As further preferred technical solution, the homofermentative lactic bacterium bag include lactobacillus acidophilus, Lactobacillus salivarius and
Lactobacillus delbrueckii；
Preferably, the volume ratio of the lactobacillus acidophilus, Lactobacillus salivarius and Lactobacillus delbrueckii is (0.5~1.5): (0.5
~1.5): (0.5~1.5)；
Preferably, the volume ratio of the lactobacillus acidophilus, Lactobacillus salivarius and Lactobacillus delbrueckii is (0.8~1.2): (0.8
~1.2): (0.8~1.2).
As further preferred technical solution, the adding proportion of the homofermentative lactic bacterium and protease be (4%~
6%): (0.1%~0.4%) mL/g；
Preferably, the adding proportion of the homofermentative lactic bacterium and protease is (4.5~5.5%): (0.2%~
0.3%) mL/g；
Preferably, bacteria containing amount >=1.0 × 10 of the homofermentative lactic bacterium8CFU/mL；
Preferably, enzyme activity >=10000U/g of the protease.
As further preferred technical solution, microbe carbon source used in the fermented feed is molasses；
Preferably, the additive amount of the molasses is 8%~12%g；
Preferably, the additive amount of the molasses is 9%~11%g.
As further preferred technical solution, base starting material used in the fermented feed includes dregs of beans, rapeseed dregs, peanut
At least one of the dregs of rice, corn flour, wheat bran, rice bran and straw bran；
And/or yeast powder used in the fermented feed includes beer yeast powder, protein Candida powder and mortierella Diding powder
At least one of；
Preferably, base starting material used in the fermented feed is dregs of beans；
Preferably, yeast powder used in the fermented feed is beer yeast powder.
As further preferred technical solution, the fermented feed is mainly prepared by following raw material: dregs of beans, molasses,
Homofermentative lactic bacterium, protease, beer yeast powder and water；
Preferably, raw material proportioning used in the fermented feed is as follows:
80%~95%g of dregs of beans, 8%~12%g of molasses, homofermentative lactic 4%~6%mL of bacterium, proteinase-10 .1%~
0.4%g, 0.3%~0.7%g of beer yeast powder, 0.2%~0.5%mL of water；
Preferably, raw material proportioning used in the fermented feed is as follows:
88%~92%g of dregs of beans, 9%~11%g of molasses, homofermentative lactic 4.5%~5.5%mL of bacterium, protease
0.2%~0.3%g, 0.4%~0.6%g of beer yeast powder, 0.3%~0.4%mL of water；
Preferably, raw material proportioning used in the fermented feed is as follows:
Dregs of beans 90%g, molasses 10%g, homofermentative lactic bacterium 5%mL, proteinase-10 .2%g, beer yeast powder 0.5%
G, water 0.34%mL.
According to another aspect of the present invention, the present invention also provides a kind of preparation methods of fermented feed, will include homotype
The lactobacillus inoculation of lactic acid fermenting bacteria and protease is inoculated in base starting material, is mixed, and fermentation obtains the fermented feed；
Wherein, the homofermentative lactic bacterium bag includes lactobacillus acidophilus, Lactobacillus salivarius, Lactobacillus delbrueckii, lactic acid sheet ball
At least two in bacterium, lactobacillus thermophilus, enterococcus faecalis and Lactococcus lactis.
As further preferred technical solution, the homofermentative lactic bacterium bag include lactobacillus acidophilus, Lactobacillus salivarius and
Lactobacillus delbrueckii；
Preferably, microbe carbon source used in the fermented feed is molasses；
Preferably, base starting material used in the fermented feed include dregs of beans, rapeseed dregs, peanut meal, corn flour, wheat bran,
At least one of rice bran and straw bran, preferably dregs of beans；
Preferably, yeast powder used in the fermented feed includes beer yeast powder, protein Candida powder and mortierella Diding
At least one of powder, preferably beer yeast powder；
Preferably, the fermented feed is mainly prepared by following raw material: dregs of beans, molasses, homofermentative lactic bacterium, egg
White enzyme, beer yeast powder and water；
Preferably, raw material proportioning used in the fermented feed is as follows:
80%~95%g of dregs of beans, 8%~12%g of molasses, homofermentative lactic 4%~6%mL of bacterium, proteinase-10 .1%~
0.4%g, 0.3%~0.7%g of beer yeast powder, 0.2%~0.5%mL of water.
As further preferred technical solution, which comprises
Sterilized dregs of beans is added in biological feedstuff bag, molasses, water, beer yeast powder and protease, mixing is added
Uniformly, homofermentative lactic bacterium is added, is uniformly mixed, fermentation obtains the fermented feed；
Preferably, the temperature of fermentation is 36~38 DEG C, preferably 36.5~37.5 DEG C；
Preferably, the time of fermentation is 46~50h, preferably 47~49h；
Preferably, further include the steps that homofermentative lactic bacterium activates；
Preferably, further include the steps that homofermentative lactic bacterium increases bacterium.
According to another aspect of the present invention, the present invention also provides a kind of above-mentioned fermented feed or the above-mentioned fermentations of utilization
The fermented feed that the preparation method of feed obtains is preparing the application in pannage.
Compared with prior art, the beneficial effects of the present invention are:
1, the present invention is combined using homofermentative lactic bacterium and protease for the first time ferments, and substantially increases lactic acid and contains
Amount, lactic acid conversion ratio is high, so that the fermented feed becomes high-content lactic fermentation feed；With green, efficient, safety, without dirt
Dye, drug residue free, the features such as nontoxic, palatability is good, easy to digest are rich in a large amount of lactic acid in feed, for improving animal
It is highly beneficial to improve animal health for the microecological balance of stomach.
2, it the present invention is based on the collaboration of homofermentative lactic bacterium and protease use, ferments, obtains under certain condition
The fermented feed arrived is rich in a large amount of lactic acid, can remarkably promote intestine beneficial bacteria colony proliferation, reduce pH value, control pernicious bacteria
Growth, keep intestinal health.Meanwhile can also improve feed flavor, and feed palatability is improved, feed nutritive value is improved, it is raw
Cost is relatively low for production, and in the case where not adding antibiotic, not only can effectively degrade the anti-nutrition such as antigen protein therein
The factor enables the effective component of feed preferably to retain, and is more conducive to promote intestinal health, improves the digestibility of feed；Together
When can be improved immunity of organisms, enhance disease resistance, promote growth, for improving the general level of the health of animal, improve livestock products
Safety have great importance.
Preparation method of the present invention, simple process is easy to operate, easy to implement, is easy to all kinds of raisers, production Feed Enterprise
Operation, application easy to spread.
Detailed description of the invention
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art
Embodiment or attached drawing needed to be used in the description of the prior art be briefly described, it should be apparent that, it is described below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, is also possible to obtain other drawings based on these drawings.
Fig. 1 is the comparison diagram of lactic acid content in the fermented feed of the embodiment of the present invention 1 and comparative example 1-4；
Fig. 2 is the HPLC chromatogram of the lactic acid concn of the fermented feed of the embodiment of the present invention 1；
Fig. 3 is the HPLC chromatogram of the lactic acid concn of the fermented feed of comparative example 1 of the present invention.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiments and examples, but this field skill
Art personnel will be understood that following embodiments and embodiment are merely to illustrate the present invention, and are not construed as limiting model of the invention
It encloses.Based on the embodiments of the present invention, obtained by those of ordinary skill in the art without making creative efforts
Every other embodiment, shall fall within the protection scope of the present invention.The person that is not specified actual conditions, according to normal conditions or manufacturer builds
The condition of view carries out.
In a first aspect, a kind of fermented feed is provided at least one embodiment, lactic acid bacteria used in the fermented feed
Kind includes homofermentative lactic bacterium and protease；
The homofermentative lactic bacterium bag includes lactobacillus acidophilus, Lactobacillus salivarius, Lactobacillus delbrueckii, Pediococcus acidilactici, thermophilic
At least two in hot lactobacillus, enterococcus faecalis and Lactococcus lactis.
Strain of the invention is purchased in the living resources preservation of food Industry in Taiwan Institute of Development Studies and research center, preservation
Number it is respectively as follows: lactobacillus acidophilus (BCRC10695), Lactobacillus salivarius (BCRC12574), Lactobacillus delbrueckii (BCRC10696), cream
Sour piece coccus (BCRC11063), lactobacillus thermophilus (BCRC12257), enterococcus faecalis (BCRC10066) and Lactococcus lactis
(BCRC10791)。
To overcome existing product technical deficiency, alleviate the bacterium that existing lactic fermentation feed uses heterolactic fermentation more
The defects of strain, lactic acid transfer efficiency is lower, its lactic acid content of the fermented feed of generation is not higher than 10% mostly, the present invention provides
A kind of fermented feed of high-content lactic acid, greatly improves lactic acid production capacity.
The present invention is acted on using the coordinated of homofermentative lactic bacterium and protease, is given full play to respective function, is made
It obtains and is rich in a large amount of lactic acid in fermented feed product of the invention, not only can effectively degrade the anti-battalion such as antigen protein therein
The factor is supported, the effective component and efficiency of feed utilization and animal digestion ability of feed are improved, is more conducive to improve the micro- life of animal intestinal tract
State balance, protects intestinal health, improves the digestibility of feed and mitigates breeding environment pollution；Immunity of organism can also be improved
Power enhances disease resistance.Meanwhile the fermented product can also improve feed flavor, improve feed palatability, improve feed nutrition valence
Value, lower production costs, with good economic efficiency and environmental benefit.
Further speaking, homofermentative lactic bacterium bag of the invention includes lactobacillus acidophilus (Lactobacillus
Acidophilus), Lactobacillus salivarius (Lactobacillus salivarius), Lactobacillus delbrueckii (Lactobacillus
Delbrueckii), Pediococcus acidilactici (Pediococcus acidilactici), lactobacillus thermophilus (Lactobacillus
Thermophilus), enterococcus faecalis Enterococcus faecalis) and Lactococcus lactis (Lactobacillus
Lactics at least two in), such as may include two kinds, three kinds, four kinds or more.Above-mentioned lactobacillus acidophilus, De Shi cream bar
Bacterium etc. belongs to homofermentative lactic bacteria, for be suitable for solid state fermentation feed excellent species, its own contain and be metabolized produce it is a variety of
Nutriment, digestive ferment and antibacterial substance are heavy to promoting the absorption of animal body Nutrients Digestion and adjusting animal physiological function to have
It acts on, in addition, above-mentioned lactobacillus has good intestinal colonisation ability, intestinal flora balance is adjusted, safeguards intestinal health.
Further, the present invention uses above-mentioned multiple homofermentative lactic bacterium compound bacterias, and combines with protease and ferment, so that newborn
Acid production ability greatly improves, and considerably increases the lactic acid concn in fermented feed.
In a preferred embodiment, the homofermentative lactic bacterium bag include lactobacillus acidophilus, Lactobacillus salivarius and
Lactobacillus delbrueckii；
Preferably, the volume ratio of the lactobacillus acidophilus, Lactobacillus salivarius and Lactobacillus delbrueckii is (0.5~1.5): (0.5
~1.5): (0.5~1.5)；
Preferably, the volume ratio of the lactobacillus acidophilus, Lactobacillus salivarius and Lactobacillus delbrueckii is (0.8~1.2): (0.8
~1.2): (0.8~1.2).
It is understood that above-mentioned homofermentative lactic bacterium includes but is not limited to lactobacillus acidophilus, Lactobacillus salivarius and moral
The mixture of family name lactobacillus three, such as can also be the mixture of Lactobacillus delbrueckii and Pediococcus acidilactici, it can also be thermophilic
The mixture of lactobacillus, enterococcus faecalis and Lactococcus lactis, can also for lactobacillus acidophilus, Lactobacillus salivarius, enterococcus faecalis and
Mixture of Lactococcus lactis etc..It is preferred that using the mixed of lactobacillus acidophilus, Lactobacillus salivarius and Lactobacillus delbrueckii three
Object is closed, mainly fermentating formula, zymotechnique etc. are described in further detail by taking the mixture as an example below the present invention, so
And it will be appreciated to those of skill in the art that it is equally applicable for above-mentioned other mixtures.
Above-mentioned homofermentative lactic bacterium is the excellent species for being suitable for solid state fermentation feed, passes through matching for above-mentioned excellent species
It closes, gives full play to respective function, can effectively inhibit feeding system, feed a large amount of miscellaneous bacterias, harmful bacteria contained in raw material
Reproduction guarantees the good fermentation of fermented feed.
The adding proportion of each homofermentative lactic bacterium of the present invention is in 1:1 or so.It is typical but non-limiting, it is thermophilic
The volume ratio of Lactobacillus lactis, Lactobacillus salivarius and Lactobacillus delbrueckii can be such as 0.5:0.6:0.5,0.5:0.5:0.6,0.5:
0.6:0.7,0.6:0.7:0.7,0.8:1:1,0.8:0.9:1,0.9:0.8:0.8,1:1:1,1.1:1.1:1.2,1.2:1.5:
1.2,1.2:1.2:1.5 or 1.5:1.5:1.4.
In a preferred embodiment, the adding proportion of the homofermentative lactic bacterium and protease be (4%~
6%): (0.1%~0.4%) mL/g；
Preferably, the adding proportion of the homofermentative lactic bacterium and protease is (4.5~5.5%): (0.2%~
0.3%) mL/g；
Preferably, bacteria containing amount >=1.0 × 10 of the homofermentative lactic bacterium8CFU/mL；
Preferably, enzyme activity >=10000U/g of the protease.
The additive amount of suitable homofermentative lactic bacterium and protease is more conducive to the two and plays coordinated effect, substantially
Improve lactic acid content.Typical but non-limiting, the adding proportion of homofermentative lactic bacterium and protease can be for example 4%:
0.1%mL/g, 5%:0.1%mL/g, 6%:0.1%mL/g, 4.5%:0.2%mL/g, 5%:0.2%mL/g, 5.5%:
0.2%mL/g, 6%:0.2%mL/g, 5.5%:0.3%mL/g or 6%:0.4%mL/g.
In a preferred embodiment, microbe carbon source used in the fermented feed is molasses；
Preferably, the additive amount of the molasses is 8%~12%g；
Preferably, the additive amount of the molasses is 9%~11%g.
Suitable molasses additive amount is more conducive to it with homofermentative lactic bacterium and protease and plays mating reaction, largely
Generate lactic acid.Typical but non-limiting, the additive amount of molasses for example can be 8%g, 8.8%g, 9%g, 9.5%g, 10%
G, 10.5%g, 11%g, 11.5%g or 12%g.
According to the present invention, using molasses as microbe carbon source, in molasses contain a large amount of sucrose and growth factor, sucrose be by
Glucose is formed with fructose, can be utilized by homofermentative lactic bacterium, efficiently be generated lactic acid.Studies have pointed out that Bacillus acidi lactici exists
In fermentation process, energy source can be used glucose as, by glucose metabolism at lactic acid, as fermentation time increases, reduction
Sugared concentration reduces, and carbon source can be efficiently used and generate metabolin by representing microorganism.Thus, can significantly it be increased using molasses as carbon source
Add lactic acid production.
Further, combine protease using above-mentioned homofermentative lactic bacterium, and add a certain amount of molasses as micro- life
Object carbon source carries out anaerobic fermentation under certain condition, can largely generate lactic acid, improves the concentration of lactic acid in feed, can be by lactic acid
Content is improved to 15% or more, and transfer efficiency can reach 90% or more.
In a preferred embodiment, base starting material used in the fermented feed includes but is not limited to dregs of beans, dish
At least one of the seed dregs of rice, peanut meal, corn flour, wheat bran, rice bran and straw bran；
And/or yeast powder used in the fermented feed includes but is not limited to beer yeast powder, protein Candida powder and ground
At least one of acremonium powder；
Preferably, base starting material used in the fermented feed is dregs of beans；
Preferably, yeast powder used in the fermented feed is beer yeast powder.
It should be understood that the present invention is not particularly limited used base starting material and yeast powder, this can be used
The conventional use of base starting material in field and yeast powder.Wherein, base starting material preferably using it is from a wealth of sources, cheap, with
Above-mentioned homofermentative lactic bacterium and the better Soybean Meal of protease mating reaction effect.Yeast powder preferably using source it is wide,
At low cost, application effect preferably live beer yeast powder.
In a preferred embodiment, the fermented feed is mainly prepared by following raw material: dregs of beans, molasses,
Homofermentative lactic bacterium, protease, beer yeast powder and water；
Preferably, raw material proportioning used in the fermented feed is as follows:
80%~95%g of dregs of beans, 8%~12%g of molasses, homofermentative lactic 4%~6%mL of bacterium, proteinase-10 .1%~
0.4%g, 0.3%~0.7%g of beer yeast powder, 0.2%~0.5%mL of water；
Preferably, raw material proportioning used in the fermented feed is as follows:
88%~92%g of dregs of beans, 9%~11%g of molasses, homofermentative lactic 4.5%~5.5%mL of bacterium, protease
0.2%~0.3%g, 0.4%~0.6%g of beer yeast powder, 0.3%~0.4%mL of water；
Preferably, raw material proportioning used in the fermented feed is as follows:
Dregs of beans 90%g, molasses 10%g, homofermentative lactic bacterium 5%mL, proteinase-10 .2%g, beer yeast powder 0.5%
G, water 0.34%mL.
It is typical but non-limiting, in the fermented feed, the additive amount of dregs of beans for example can for 80%g, 82%g,
84%g, 85%g, 86%g, 88%g, 90%g, 92%g, 94%g or 95%g；The additive amount of molasses for example can for 8%g,
8.8%g, 9%g, 9.5%g, 10%g, 10.5%g, 11%g, 11.5%g or 12%g；The additive amount of homofermentative lactic bacterium
It such as can be 4%mL, 4.2%mL, 4.5%gmL, 4.5%mL, 5%mL, 5.2%mL, 5.5%mL, 5.8%mL or 6%
mL；The additive amount of protease for example can for 0.1%g, 0.15%g, 0.2%g, 0.25%g, 0.3%g, 0.35%g or
0.4%g；The additive amount of beer yeast powder for example can be 0.3%g, 0.4%g, 0.5%g, 0.6%g or 0.7%g；Water adds
Dosage for example can for 0.2%mL, 0.25%mL, 0.3%gmL, 0.34%mL, 0.35%mL, 0.36%mL, 0.38%mL,
0.4%mL, 0.45%mL or 0.5%mL.
It is fermented using above-mentioned fermentating formula, homofermentative lactic bacterium and protease and molasses etc. can be given full play to
Mating reaction greatly improves lactic acid production capacity, improves lactic acid transfer efficiency, can also reduce production cost, and improve fermentation
Production level reduces influence of the unnecessary substance for quality of fermentation broth and ferment effect, improves the stability of production.
Second aspect provides a kind of preparation method of fermented feed at least one embodiment, will include homolactic
The lactobacillus inoculation of zymophyte and protease is inoculated in base starting material, is mixed, and fermentation obtains the fermented feed；
Wherein, the homofermentative lactic bacterium bag includes lactobacillus acidophilus, Lactobacillus salivarius, Lactobacillus delbrueckii, lactic acid sheet ball
At least two in bacterium, lactobacillus thermophilus, enterococcus faecalis and Lactococcus lactis.
The present invention carries out anaerobic fermentation by using homofermentative lactic bacterium joint protease under given conditions, alleviates
Lactic acid content caused by selecting bad and zymotechnique incomplete due to lactobacillus inoculation in the prior art is low, lactic acid transfer efficiency
The defects of not high and fermentation time is long.
It is rich in a large amount of lactic acid by the obtained fermented feed of this method, intestine beneficial bacteria colony increasing can be remarkably promoted
It grows, reduces pH value, control the growth of pernicious bacteria, keep intestinal health.Meanwhile can also improve feed flavor, it is suitable to improve feed
Mouth property, raising feed nutritive value, lower production costs, and in the case where not adding antibiotic, not only can effectively it drop
The anti-nutritional factors such as antigen protein therein are solved, the effective component of feed is enable preferably to retain, are more conducive to promote enteron aisle
Health improves the digestibility of feed；Immunity of organisms can be improved simultaneously, enhance disease resistance, promote growth, it is dynamic for improving
The general level of the health of object, the safety for improving livestock products have great importance.
The addition of fermented feed preparation process of the present invention and final products antibiotic-free, it is green safe, it is environmental-friendly, and
Simple process, it is easy to operate, easy to implement, it is easy to all kinds of raisers, production Feed Enterprise operation, application easy to spread.
In a preferred embodiment, the homofermentative lactic bacterium bag include lactobacillus acidophilus, Lactobacillus salivarius and
Lactobacillus delbrueckii；
Preferably, microbe carbon source used in the fermented feed is molasses；
Preferably, base starting material used in the fermented feed include dregs of beans, rapeseed dregs, peanut meal, corn flour, wheat bran,
At least one of rice bran and straw bran, preferably dregs of beans；
Preferably, yeast powder used in the fermented feed includes beer yeast powder, protein Candida powder and mortierella Diding
At least one of powder, preferably beer yeast powder；
Preferably, the fermented feed is mainly prepared by following raw material: dregs of beans, molasses, homofermentative lactic bacterium, egg
White enzyme, beer yeast powder and water；
Preferably, raw material proportioning used in the fermented feed is as follows:
80%~95%g of dregs of beans, 8%~12%g of molasses, homofermentative lactic 4%~6%mL of bacterium, proteinase-10 .1%~
0.4%g, 0.3%~0.7%g of beer yeast powder, 0.2%~0.5%mL of water.
It should be noted that used by fermented feed described in fermentation raw material used by preparation method and first aspect
Fermentation raw material is identical, the two be based on the same inventive concept, for the duplicate content of first aspect, the present invention is herein no longer
It repeats.
In a preferred embodiment, which comprises
Sterilized dregs of beans is added in biological feedstuff bag, molasses, water, beer yeast powder and protease, mixing is added
Uniformly, homofermentative lactic bacterium is added, is uniformly mixed, fermentation obtains the fermented feed；
Preferably, the temperature of fermentation is 36~38 DEG C, preferably 36.5~37.5 DEG C, further preferably 37 DEG C；
Preferably, the time of fermentation is 46~50h, preferably 47~49h, further preferably 48h；
Preferably, further include the steps that homofermentative lactic bacterium activates；
Preferably, further include the steps that homofermentative lactic bacterium increases bacterium.
It is understood that processing commonly used in the art can be used in the step of above-mentioned homofermentative lactic bacterium activation and increasing bacterium
Mode is handled, and there is no special restriction on this by the present invention.
In order to help to further understand the present invention, technical solution of the present invention is carried out now in conjunction with preferred embodiment detailed
Explanation.
[preparation of lactobacillus suspension]
(1) Bacillus acidi lactici activates
A) respectively by lactobacillus acidophilus (Lactobacillus acidophilus), Lactobacillus salivarius
The freeze-drying pipe of (Lactobacillus salivarius) and Lactobacillus delbrueckii (Lactobacillus delbrueckii) is opened
Envelope, top are put to heating on flame, and the outer tube top of lower rupture is struck with file, takes out sheathing paper and inner tube, sterilized tweezers
Gently press from both sides out cotton plug.
B) it takes 0.3~0.5mL sterile water to instill inner tube, and slightly shakes, stand 30 minutes or so, obtain bacterium solution.
C) take the above-mentioned bacterium solution drop of 0.1~0.2mL in artificial preparation and sterilized MRS broth agar culture medium (Man
Rogosa sharpe agar, MRS agar) in, the purity of strain and the feelings of activation are detected after Anaerobic culturel 24 hours in 37 DEG C
Shape.
(2) Bacillus acidi lactici increases bacterium
A) using meet GB19644-2010 standard and antibiotic-free pollution skimmed milk power and distilled water, according to 1:8~
(powder water) proportional arrangement of the 1:9 skimmed milk power liquid culture medium to be processed into 3 bottles of 250mL.
B) above-mentioned skimmed milk power liquid culture medium to be processed is thoroughly mixed uniformly, be then placed in Sterilization Kettle with
Pressure 1.2kg/cm2, 121 DEG C of temperature, the time 15 minutes conditions sterilize；Then it cools down at room temperature, obtains 3 bottles of degreasings
Milk powder liquid culture medium.
C) lactobacillus acidophilus, the Lactobacillus salivarius that 1mL has been activated respectively is added into above-mentioned 3 bottles of skimmed milk power liquid culture mediums
With Lactobacillus delbrueckii bacterium solution, after mixing in temperature be 37 DEG C, revolving speed be 180rpm under the conditions of Anaerobic culturel 24 hours；
D) lactic acid bacteria number is detected with colony counting method, bacteria containing amount is not less than 1.0 × 108CFU/mL, which is considered as, increases bacterium success, if
It is then reactivated lower than this standard, until reaching this standard.
E) three kinds of bacterium are mixed, becomes lactobacillus suspension.
[formula of anaerobic solid-state fermentation]
Raw material proportioning used in fermented feed is shown in Table 1:
1 fermented feed formula table of table
| Raw material | Additive amount |
| Soybean Meal (g) | 90% |
| Molasses (g) | 10% |
| The lactobacillus suspension (mL) of bacterium is increased | 5% |
| Albumen enzyme powder (g) | 0.2% |
| Beer yeast powder (g) living | 0.5% |
| Pure water (mL) | 0.34% |
[preparation of fermented feed]
A) scale sterilized Soybean Meal is got well to be fitted into the biological feedstuff bag of tool one-way exhaust valve；
B) molasses, pure water, beer yeast powder living and albumen enzyme powder are added into above-mentioned biological feedstuff bag, and (activity is not less than
10000U/g), it is mixed evenly；
C) lactobacillus suspension for having increased bacterium of mixing is added into biological feedstuff bag again, is mixed evenly；
D) it is sealed with sealing machine, is put into 37 DEG C of incubators and carries out anaerobic fermentation 48 hours；
E) it after fermenting, is taken out from incubator, it is 25 DEG C of cooled to room temperature, to be analyzed, obtain fermented feed.
In the present invention, the analyzing detecting method of lactic acid concn is examined according to the method for GB/T23877-2009 in feed
It surveys.
The third aspect provides the fermented feed described in more than one at least one embodiment or utilizes the fermentation
The fermented feed that the preparation method of feed obtains is preparing the application in pannage.
The diarrhea that above-mentioned fermented feed can be used for promoting the intestinal health of piglet or growth type pig, improve constipation, reduce pig
Rate, strengthen immunity, improve resistance and/or improve piglet incubation rate etc..
The fermented feed that the present invention provides, such as can be used for improving weanling pig intestinal health.The feed can
Promote intestine of young pigs profitable strain proliferation, control the growth of pernicious bacteria, keeps intestinal health；Catarrhal diarrhea occurs for piglet
When, intestinal microecology balance can be corrected, harmful microorganism growth is inhibited, protects intestinal wall chorion, timely antidiarrheal promotes abdomen
Enteric recovery after rushing down.And the solid state fermentation feed efficiency prepared is significant, can not only improve efficiency of feed utilization, promotes piglet
Growth, and can also improve and maintain its intestinal flora balance, pigling immunity and premunition are improved, and be effectively improved piglet
Diarrhea increases its survival rate.The addition of fermented feed preparation process and final products antibiotic-free of the invention, it is green safe,
It is environmental-friendly.
Combined with specific embodiments below, comparative example and attached drawing, the invention will be further described.
Embodiment 1
A kind of fermented feed, raw material proportioning used in the fermented feed, as shown in table 2:
The fermented feed formula table of 2 embodiment 1 of table
| Raw material | Additive amount |
| Dregs of beans (g) | 90% |
| Molasses (g) | 10% |
| Homofermentative lactic bacterium bacterium solution (mL) | 5% |
| Albumen enzyme powder (g) | 0.2% |
| Beer yeast powder (g) living | 0.5% |
| Pure water (mL) | 0.34% |
Wherein, the homofermentative lactic bacterium bacterium solution include volume ratio be the lactobacillus acidophilus of 1:1:1, Lactobacillus salivarius and
Lactobacillus delbrueckii.
The preparation method of above-mentioned fermented feed includes:
(1) homofermentative lactic bacterium activates
A) the freeze-drying pipe of lactobacillus acidophilus, Lactobacillus salivarius and Lactobacillus delbrueckii is broken a seal respectively, top is put to flame
The outer tube top of lower rupture is struck in heating with file, takes out sheathing paper and inner tube, sterilized tweezers gently press from both sides out cotton plug；
B) it takes 0.5mL sterile water to instill inner tube, and slightly shakes, stand 30 minutes, obtain bacterium solution；
C) take the above-mentioned bacterium solution drop of 0.2mL in artificial preparation and sterilized MRS broth agar culture medium, in 37 DEG C of anaerobism
The purity of strain and the situation of activation are detected after culture 24 hours.
(2) homofermentative lactic bacterium increases bacterium
A) using the skimmed milk power and distilled water for meeting GB19644-2010 standard and antibiotic-free pollution, according to 1:8's
(powder water) the proportional arrangement skimmed milk power liquid culture medium to be processed into 3 bottles of 250mL；
B) above-mentioned skimmed milk power liquid culture medium to be processed is thoroughly mixed uniformly, be then placed in Sterilization Kettle with
Pressure 1.2kg/cm2, 121 DEG C of temperature, the time 15 minutes conditions sterilize；Then it cools down at room temperature, obtains 3 bottles of degreasings
Milk powder liquid culture medium；
C) lactobacillus acidophilus, the Lactobacillus salivarius that 1mL has been activated respectively is added into above-mentioned 3 bottles of skimmed milk power liquid culture mediums
With Lactobacillus delbrueckii bacterium solution, after mixing Anaerobic culturel 24 hours under the conditions of temperature is 37 DEG C, revolving speed is 180rpm；
D) lactic acid bacteria number is detected with colony counting method, bacteria containing amount is not less than 1.0 × 108CFU/mL, which is considered as, increases bacterium success, if
It is then reactivated lower than this standard, until reaching this standard.
E) three kinds of bacterium are mixed, obtains homofermentative lactic bacterium bacterium solution.
(3) preparation of fermented feed
A) scale sterilized dregs of beans is got well to be fitted into the biological feedstuff bag of tool one-way exhaust valve；
B) molasses, pure water, beer yeast powder living and albumen enzyme powder are added into above-mentioned biological feedstuff bag, and (activity is not less than
10000U/g, similarly hereinafter), it is mixed evenly；
C) the homofermentative lactic bacterium bacterium solution of mixing is added into biological feedstuff bag again, is mixed evenly；
D) it is sealed with sealing machine, is put into 37 DEG C of incubators and carries out anaerobic fermentation 48 hours；
E) it after fermenting, is taken out from incubator, 25 DEG C of cooled to room temperature, obtains fermented feed.
Through analysis detection, lactic acid content is about 16% in the fermented feed that embodiment 1 obtains, transfer efficiency about 90%.
Comparative example 1
A kind of fermented feed, the difference from embodiment 1 is that being added without albumen enzyme powder in formula；The preparation of the fermented feed
Method is similar to Example 1, is a difference in that and is added without albumen enzyme powder.
Comparative example 2-4
The formula of a kind of fermented feed, the fermented feed is identical as the formula of embodiment 1；
The preparation method of the fermented feed is similar to Example 1, except that the change of fermentation time；Embodiment 2-
4 fermentation time is respectively 12 hours, 24 hours and 36 hours.
Fig. 1 shows the comparison of lactic acid content in the fermented feed of embodiment 1 and comparative example 1-4.It can from Fig. 1
Out, under the same conditions, albumen enzyme fermentation is added, and does not add albumen enzyme fermentation, lactic acid content phase in obtained feed
Difference is larger, and the embodiment of the present invention 1 is fermented by using homofermentative lactic bacterium joint protease, its lactic acid contains after 48 hours
Amount can reach about 16%；And comparative example 1 uses only homofermentative lactic bacterium under the same conditions, is not added with albumen enzyme fermentation,
Its lactic acid content is only 7.5% after 48 hours.That is, the effect of the joint protease anaerobic fermentation of embodiment 1 is higher than comparative example 1
2 times only with homofermentative lactic bacterium effect.
Fig. 2 is the HPLC chromatogram of the lactic acid concn of the fermented feed of embodiment 1, and Fig. 3 is the fermented feed of comparative example 1
The HPLC chromatogram of lactic acid concn.With reference to Fig. 1-Fig. 3, it is logical that the present invention can be seen that by the comparison of embodiment 1 and comparative example 1
It crosses and anaerobic fermentation is carried out using homofermentative lactic bacterium joint protease under certain condition, improve lactic acid content, alleviate
The lactic acid content of the existing fermented feed problem low not higher than 10%, lactic acid transfer efficiency.Further speaking, foundation of the present invention
Microbial metabolism generate organic acid characteristic, select protease combined ferment, can in low pH effective aminosal, benefit
It is utilized in microbial fermentation.And a certain amount of protease is added in dregs of beans, can increase free aminoacid content in dregs of beans, it will
Protein degradation is peptide, amino acid in dregs of beans, needs nutrition to provide Institute of Micro-biology, has the effect of promoting fermentation.After fermentation
Phase, lactic acid bacteria become dominant strain and using carbohydate metabolism at lactic acid.In addition, being sent out with molasses inoculation homofermentative lactic bacterium
Ferment can also increase lactic acid concn, and lactic acid concn is positively correlated with molasses addition, because molasses contain a large amount of sucrose and growth factor,
Sucrose is made of glucose and fructose, can be utilized by homolactic bacterium, efficiently be generated lactic acid, thus using molasses as carbon source
Lactic acid production can be dramatically increased.Therefore, the present invention be suitble to growth conditions under, homofermentative lactic bacterium combine protease effectively into
Row lactic fermentation, largely generates lactic acid, improves the concentration of lactic acid in feed.
In addition, can also be seen that influence of the different fermentation times for lactic acid content from Fig. 1, fermentation time is too short,
Then lactic acid content is also less, such as 12 hours its lactic acid contents of fermentation in comparative example 2 are only about 11.5%；Thus, the present invention
Preferably fermentation time is set in 48 hours or so, the content of lactic acid can be improved.
Embodiment 2
A kind of fermented feed, raw material proportioning used in the fermented feed, as shown in table 3:
The fermented feed formula table of 3 embodiment 2 of table
Wherein, the homofermentative lactic bacterium bacterium solution include volume ratio be the lactobacillus acidophilus of 1:1:1, Lactobacillus salivarius and
Lactobacillus delbrueckii.
The preparation method of the fermented feed is same as Example 1.
Through analysis detection, lactic acid content is about 15.6% in the fermented feed that embodiment 2 obtains, transfer efficiency about 88%.
Embodiment 3
A kind of fermented feed, raw material proportioning used in the fermented feed, as shown in table 4:
The fermented feed formula table of 4 embodiment 3 of table
| Raw material | Additive amount |
| Dregs of beans (g) | 88% |
| Molasses (g) | 9% |
| Homofermentative lactic bacterium bacterium solution (mL) | 4.5% |
| Albumen enzyme powder (g) | 0.25% |
| Beer yeast powder (g) living | 0.4% |
| Pure water (mL) | 0.3% |
Wherein, the homofermentative lactic bacterium bacterium solution includes Lactobacillus delbrueckii, the lactic acid sheet ball that volume ratio is 1:0.9:0.9
Bacterium and lactobacillus thermophilus.
The preparation method of the fermented feed is same as Example 1.
Through analysis detection, lactic acid content is about 14.8% in the fermented feed that embodiment 3 obtains, transfer efficiency about 77%.
Embodiment 4
A kind of fermented feed, raw material proportioning used in the fermented feed, as shown in table 5:
The fermented feed formula table of 5 embodiment 4 of table
| Raw material | Additive amount |
| Dregs of beans and rapeseed dregs (g) | 85% |
| Molasses (g) | 8% |
| Homofermentative lactic bacterium bacterium solution (mL) | 4% |
| Albumen enzyme powder (g) | 0.15% |
| Protein Candida powder (g) | 0.35% |
| Pure water (mL) | 0.28% |
Wherein, it is the Pediococcus acidilactici of 0.9:1:1:1.1, thermophilic cream that the homofermentative lactic bacterium bacterium solution, which includes volume ratio,
Bacillus, enterococcus faecalis and Lactococcus lactis.
The preparation method of the fermented feed is same as Example 1.
Through analysis detection, lactic acid content is about 14% in the fermented feed that embodiment 4 obtains, transfer efficiency about 73%.
Embodiment 5
A kind of fermented feed, raw material proportioning used in the fermented feed, as shown in table 6:
The fermented feed formula table of 6 embodiment 5 of table
| Raw material | Additive amount |
| Dregs of beans and peanut meal (g) | 95% |
| Molasses (g) | 11.5% |
| Homofermentative lactic bacterium bacterium solution (mL) | 6% |
| Albumen enzyme powder (g) | 0.4% |
| Beer yeast powder (g) living | 0.65% |
| Pure water (mL) | 0.5% |
Wherein, it is the lactobacillus acidophilus of 1:1:1.2, Lactobacillus salivarius that the homofermentative lactic bacterium bacterium solution, which includes volume ratio,
And lactobacillus thermophilus.
The preparation method of the fermented feed is same as Example 1.
Through analysis detection, lactic acid content is about 17.2% in the fermented feed that embodiment 5 obtains, transfer efficiency about 88%.
Embodiment 6
A kind of fermented feed, raw material proportioning used in the fermented feed is same as Example 1, the difference is that fermentation is raised
The preparation method of material.
The preparation method of the fermented feed of the present embodiment includes:
(1) homofermentative lactic bacterium activates
A) the freeze-drying pipe of lactobacillus acidophilus, Lactobacillus salivarius and Lactobacillus delbrueckii is broken a seal respectively, top is put to flame
The outer tube top of lower rupture is struck in heating with file, takes out sheathing paper and inner tube, sterilized tweezers gently press from both sides out cotton plug；
B) it takes 0.4mL sterile water to instill inner tube, and slightly shakes, stand 30 minutes, obtain bacterium solution；
C) take the above-mentioned bacterium solution drop of 0.1mL in artificial preparation and sterilized MRS broth agar culture medium, in 37 DEG C of anaerobism
The purity of strain and the situation of activation are detected after culture 24 hours.
(2) homofermentative lactic bacterium increases bacterium
A) using the skimmed milk power and distilled water for meeting GB19644-2010 standard and antibiotic-free pollution, according to 1:9's
(powder water) the proportional arrangement skimmed milk power liquid culture medium to be processed into 3 bottles of 250mL；
B) above-mentioned skimmed milk power liquid culture medium to be processed is thoroughly mixed uniformly, be then placed in Sterilization Kettle with
Pressure 1.2kg/cm2, 121 DEG C of temperature, the time 15 minutes conditions sterilize；Then it cools down at room temperature, obtains 3 bottles of degreasings
Milk powder liquid culture medium；
C) lactobacillus acidophilus, the Lactobacillus salivarius that 1mL has been activated respectively is added into above-mentioned 3 bottles of skimmed milk power liquid culture mediums
With Lactobacillus delbrueckii bacterium solution, after mixing Anaerobic culturel 24 hours under the conditions of temperature is 37 DEG C, revolving speed is 180rpm；
D) lactic acid bacteria number is detected with colony counting method, bacteria containing amount is not less than 1.0 × 108CFU/mL, which is considered as, increases bacterium success, if
It is then reactivated lower than this standard, until reaching this standard.
E) three kinds of bacterium are mixed, obtains homofermentative lactic bacterium bacterium solution.
(3) preparation of fermented feed
A) scale sterilized dregs of beans is got well to be fitted into the biological feedstuff bag of tool one-way exhaust valve；
B) molasses, pure water, beer yeast powder living and albumen enzyme powder are added into above-mentioned biological feedstuff bag, and (activity is not less than
10000U/g, similarly hereinafter), it is mixed evenly；
C) the homofermentative lactic bacterium bacterium solution of mixing is added into biological feedstuff bag again, is mixed evenly；
D) it is sealed with sealing machine, is put into 38 DEG C of incubators and carries out anaerobic fermentation 46 hours；
E) it after fermenting, is taken out from incubator, 25 DEG C of cooled to room temperature, obtains fermented feed.
Through analysis detection, lactic acid content is about 17.2% in the fermented feed that embodiment 6 obtains, transfer efficiency about 90%.
Embodiment 7
A kind of fermented feed, raw material proportioning used in the fermented feed is same as Example 1, the difference is that fermentation is raised
The preparation method of material.
The preparation method of the fermented feed of the present embodiment includes:
(1) homofermentative lactic bacterium activates
A) the freeze-drying pipe of lactobacillus acidophilus, Lactobacillus salivarius and Lactobacillus delbrueckii is broken a seal respectively, top is put to flame
The outer tube top of lower rupture is struck in heating with file, takes out sheathing paper and inner tube, sterilized tweezers gently press from both sides out cotton plug；
B) it takes 0.3mL sterile water to instill inner tube, and slightly shakes, stand 25 minutes, obtain bacterium solution；
C) it takes the above-mentioned bacterium solution drop of 0.15mL in artificial preparation and sterilized MRS broth agar culture medium, detests in 37 DEG C
The purity of strain and the situation of activation are detected after oxygen culture 24 hours.
(2) homofermentative lactic bacterium increases bacterium
A) using the skimmed milk power and distilled water for meeting GB19644-2010 standard and antibiotic-free pollution, according to 1:8.5
(powder water) proportional arrangement into 3 bottles of 250mL skimmed milk power liquid culture medium to be processed；
B) above-mentioned skimmed milk power liquid culture medium to be processed is thoroughly mixed uniformly, be then placed in Sterilization Kettle with
Pressure 1.2kg/cm2, 121 DEG C of temperature, the time 15 minutes conditions sterilize；Then it cools down at room temperature, obtains 3 bottles of degreasings
Milk powder liquid culture medium；
C) lactobacillus acidophilus, the Lactobacillus salivarius that 1mL has been activated respectively is added into above-mentioned 3 bottles of skimmed milk power liquid culture mediums
With Lactobacillus delbrueckii bacterium solution, after mixing Anaerobic culturel 24 hours under the conditions of temperature is 37 DEG C, revolving speed is 180rpm；
D) lactic acid bacteria number is detected with colony counting method, bacteria containing amount is not less than 1.0 × 108CFU/mL, which is considered as, increases bacterium success, if
It is then reactivated lower than this standard, until reaching this standard.
E) three kinds of bacterium are mixed, obtains homofermentative lactic bacterium bacterium solution.
(3) preparation of fermented feed
A) scale sterilized dregs of beans is got well to be fitted into the biological feedstuff bag of tool one-way exhaust valve；
B) molasses, pure water, beer yeast powder living and albumen enzyme powder are added into above-mentioned biological feedstuff bag, and (activity is not less than
10000U/g, similarly hereinafter), it is mixed evenly；
C) the homofermentative lactic bacterium bacterium solution of mixing is added into biological feedstuff bag again, is mixed evenly；
D) it is sealed with sealing machine, is put into 36 DEG C of incubators and carries out anaerobic fermentation 50 hours；
E) it after fermenting, is taken out from incubator, 25 DEG C of cooled to room temperature, obtains fermented feed.
Through analysis detection, lactic acid content is about 18% in the fermented feed that embodiment 7 obtains, transfer efficiency about 90%.
Experimental example
On certain pig farm of Henan, selection weanling pig is tested, and test is separated from milk boar using 108 21 ages in days, average first
Weight is 7.93 kilograms.Machine is met to be divided into nine processing groups according to weight, test group and control group 1 admix implementation according to 5% dosage respectively
The fermented feed that example 1-7 and comparative example 1 are prepared.Every processing group respectively has 3 columns using column as recurring unit, and every column raises 4
Piglet.Test 4 weeks by a definite date adopts during test and food mode is appointed to give feed and drinking-water, daily tour 2-3 times, more depending on feeding situation
Change drinking-water and supplement feed.The 1st day first platform scale initial weight is tested, and the 28th day weighing is primary, calculates average daily gain
(averagedailygain, abbreviation ADG), average daily gain (averagedailyfeedintake, abbreviation ADFI), feed
Efficiency (feedconversionrate, FCR), and calculate diarrhea rate.Control group 2 is blank control group, is not spiked with fermented feed.
Experimental result is as shown in table 7.
Table 7 feeds influence of the fermented feed to each index of weanling pig
Note: numerical value is indicated in table with average value (± standard deviation).
As can be seen from Table 7,1-7 of the embodiment of the present invention has higher average day compared to blank control and comparative example 1
Weight gain, feed intake and feed conversion rate.Illustrate fermented feed provided by the invention can slow down piglet to the allergic reaction of soybean with
Promote growth performance；And grice diarrhoea rate is reduced, there is the effect of improving intestine of young pigs bacterium phase and adjusting immune response.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (10)
1. a kind of fermented feed, which is characterized in that lactobacillus inoculation used in the fermented feed include homofermentative lactic bacterium and
Protease；
The homofermentative lactic bacterium bag includes lactobacillus acidophilus, Lactobacillus salivarius, Lactobacillus delbrueckii, Pediococcus acidilactici, thermophilic cream
At least two in bacillus, enterococcus faecalis and Lactococcus lactis.
2. fermented feed according to claim 1, which is characterized in that the homofermentative lactic bacterium bag includes acidophilus cream bar
Bacterium, Lactobacillus salivarius and Lactobacillus delbrueckii；
Preferably, the volume ratio of the lactobacillus acidophilus, Lactobacillus salivarius and Lactobacillus delbrueckii is (0.5~1.5): (0.5~
1.5): (0.5~1.5)；
Preferably, the volume ratio of the lactobacillus acidophilus, Lactobacillus salivarius and Lactobacillus delbrueckii is (0.8~1.2): (0.8~
1.2): (0.8~1.2).
3. fermented feed according to claim 1, which is characterized in that the addition of the homofermentative lactic bacterium and protease
Ratio is (4%~6%): (0.1%~0.4%) mL/g；
Preferably, the adding proportion of the homofermentative lactic bacterium and protease is (4.5~5.5%): (0.2%~0.3%)
mL/g；
Preferably, bacteria containing amount >=1.0 × 10 of the homofermentative lactic bacterium8CFU/mL；
Preferably, enzyme activity >=10000U/g of the protease.
4. fermented feed according to claim 1, which is characterized in that microbe carbon source used in the fermented feed is sugar
Honey；
Preferably, the additive amount of the molasses is 8%~12%g；
Preferably, the additive amount of the molasses is 9%~11%g.
5. fermented feed according to claim 1, which is characterized in that base starting material used in the fermented feed includes beans
At least one of the dregs of rice, rapeseed dregs, peanut meal, corn flour, wheat bran, rice bran and straw bran；
And/or yeast powder used in the fermented feed includes in beer yeast powder, protein Candida powder and mortierella Diding powder
It is at least one；
Preferably, base starting material used in the fermented feed is dregs of beans；
Preferably, yeast powder used in the fermented feed is beer yeast powder.
6. described in any item fermented feeds according to claim 1~5, which is characterized in that the fermented feed is mainly by following
Raw material is prepared: dregs of beans, molasses, homofermentative lactic bacterium, protease, beer yeast powder and water；
Preferably, raw material proportioning used in the fermented feed is as follows:
80%~95%g of dregs of beans, 8%~12%g of molasses, homofermentative lactic 4%~6%mL of bacterium, proteinase-10 .1%~
0.4%g, 0.3%~0.7%g of beer yeast powder, 0.2%~0.5%mL of water；
Preferably, raw material proportioning used in the fermented feed is as follows:
88%~92%g of dregs of beans, 9%~11%g of molasses, homofermentative lactic 4.5%~5.5%mL of bacterium, proteinase-10 .2%~
0.3%g, 0.4%~0.6%g of beer yeast powder, 0.3%~0.4%mL of water；
Preferably, raw material proportioning used in the fermented feed is as follows:
Dregs of beans 90%g, molasses 10%g, homofermentative lactic bacterium 5%mL, proteinase-10 .2%g, beer yeast powder 0.5%g, water
0.34%mL.
7. a kind of preparation method of fermented feed, which is characterized in that by the lactic acid bacteria comprising homofermentative lactic bacterium and protease
Kind is inoculated in base starting material, is mixed, and fermentation obtains the fermented feed；
Wherein, the homofermentative lactic bacterium bag includes lactobacillus acidophilus, Lactobacillus salivarius, Lactobacillus delbrueckii, Pediococcus acidilactici, thermophilic
At least two in hot lactobacillus, enterococcus faecalis and Lactococcus lactis.
8. the preparation method of fermented feed according to claim 7, which is characterized in that the homofermentative lactic bacterium bag includes
Lactobacillus acidophilus, Lactobacillus salivarius and Lactobacillus delbrueckii；
Preferably, microbe carbon source used in the fermented feed is molasses；
Preferably, base starting material used in the fermented feed includes dregs of beans, rapeseed dregs, peanut meal, corn flour, wheat bran, rice bran
At least one of with straw bran, preferably dregs of beans；
Preferably, yeast powder used in the fermented feed includes in beer yeast powder, protein Candida powder and mortierella Diding powder
At least one, preferably beer yeast powder；
Preferably, the fermented feed is mainly prepared by following raw material: dregs of beans, molasses, homofermentative lactic bacterium, albumen
Enzyme, beer yeast powder and water；
Preferably, raw material proportioning used in the fermented feed is as follows:
80%~95%g of dregs of beans, 8%~12%g of molasses, homofermentative lactic 4%~6%mL of bacterium, proteinase-10 .1%~
0.4%g, 0.3%~0.7%g of beer yeast powder, 0.2%~0.5%mL of water.
9. the preparation method of fermented feed according to claim 7 or 8, which is characterized in that the described method includes:
Sterilized dregs of beans is added in biological feedstuff bag, molasses, water, beer yeast powder and protease is added, is uniformly mixed,
Homofermentative lactic bacterium is added, is uniformly mixed, fermentation obtains the fermented feed；
Preferably, the temperature of fermentation is 36~38 DEG C, preferably 36.5~37.5 DEG C；
Preferably, the time of fermentation is 46~50h, preferably 47~49h；
Preferably, further include the steps that homofermentative lactic bacterium activates；
Preferably, further include the steps that homofermentative lactic bacterium increases bacterium.
10. the system of the described in any item fermented feeds of claim 1~6 or the described in any item fermented feeds of claim 7~9
The fermented feed that Preparation Method obtains is preparing the application in pannage.
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Application publication date: 20181221 |