CN108402337A - One seed shrimp ferment and the preparation method and application thereof - Google Patents
One seed shrimp ferment and the preparation method and application thereof Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/41—Pediococcus
- A23V2400/413—Acidilactici
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses seed shrimp ferment and the preparation method and application thereof, which includes that mass ratio is 9:1 solid medium and fermentation are wrapped, and the solid medium includes following components according to mass parts：25 parts 35 parts of dregs of beans, 10 parts 20 parts of corn, 10 parts 20 parts of wheat-middlings, 5 parts 15 parts of peanut meal, 5 parts 15 parts of fish meal, 3 parts 8 parts of molasses, 0.5 part 1.5 parts of multidimensional, 0.5 part 1.5 parts of more mines, 1 part 5 parts of astragalus polyose；The fermentation packet includes following components according to mass parts：75 85 parts of lactic acid bacteria, 5 parts 15 parts of bacillus, 5 parts 15 parts of saccharomycete.The characteristics of ferment of the present invention proportioning is appropriate, has nutritional ingredient comprehensive, and food calling power is strong, water transfer steady water, promotes the fast-growth of shrimp, reduces feed coefficient, improve the immunity of shrimp, adjust breeding water body stability, reduce cultivation risk, improve cultivation success rate.
Description
Technical field
The invention belongs to aquatic feeds and Raw material processing technical fields, specifically, being related to a seed shrimp ferment and its preparation
Method and application.
Background technology
Mai Kangsen academician proposes：Aquaculture enters the ferment epoch.The product to ferment now in aquaculture increasingly by
To attention.Penaeus Vannmei growth is fast, dressing percentage is high, resistance is strong, especially wide to salinity adaptation range, can be supported in nearly fresh water
It grows, it has also become whole world cultured output is highest to species.But as people pursue the blindness of high yield, breeding density
Be continuously increased, daily ration, feeding quantity greatly caused by the problems such as cultivation water environment is continuous worsening, communicable disease large-scale outbreak, it is serious to restrict
The further development of shrimp culture industry.
Lactic acid bacteria includes two kinds of Pediococcus acidilactici and Lactobacillus casei, wherein Pediococcus acidilactici adjusts gastrointestinal bacterial flora, dimension
Hold intestinal microecology balance.There is antagonistic action to pathogenic microorganism in animal body, inhibit to contestable pathogenic microorganism, increases
The immune function of strong animal body, generates beneficial metabolite, activates the activity of acid protease, participate in the new old generation of body
It thanks, prevents harmful substance from generating.
Lactobacillus casei, which has, promotes cell division, generates antibody mediated immunity, enhances the effect of animal body immunity.Cheese breast
Bacillus can inhibit and kill many spoilage organisms and pathogenic bacteria in feed, effectively prevent feed putrid and deteriorated.
Bacillus has the function of antibacterial, can consume the free oxygen in environment rapidly, causes enteron aisle hypoxemia, promotes beneficial
Anaerobic bacteria grows, and generates the organic acids such as lactic acid, reduces enteron aisle pH value, inhibits other pathogenic bacteria to grow indirectly, can also enhance
Cellular immunity and humoral immune function improve herd immunity.Secreted from bacillus much inhale by extracellular enzyme auxiliary prawn digestion
It receives.
Saccharomycete makes feed have aromatic odor by fermentation, and food calling, saccharomycete is promoted to contain abundant protein (30-
40% or so), the substances such as B family vitamin, amino acid, the protein supplements as animal feed.It can promote the life of animal
Length development, shortens feeding period, increases meat amount and egg amount, improving pork quality and raising lean meat percentage, improves the glossiness of fur, and can increase
The disease resistance of strong cub fowl.
There is the fermented feed that similar shrimp uses on the market now, but there are many problems, such as：Strain collocation ratio
Unreasonable, fermentation method and zymotechnique are unreasonable, and culture medium is of poor quality, the bad control of fermenting raw materials degree and grain size
Palatability is poor etc..
Invention content
In view of this, the present invention is directed to above-mentioned problem, seed shrimp ferment and the preparation method and application thereof, this hair are provided
After usage, shrimp ingests to be obviously increased bright ferment, enteron aisle blackening is thicker, body colour is bright, vigor enhancing, survival rate and cultivation
Yield obviously increases.
In order to solve the above-mentioned technical problem, the invention discloses a seed shrimp ferment, including mass ratio is 9:1 solid culture
Base and fermentation are wrapped, and solid medium includes following components according to mass parts：25 parts -35 parts of dregs of beans, 10 parts -20 parts of corn, wheat-middlings
10 parts -20 parts, 5 parts -15 parts of peanut meal, 5 parts -15 parts of fish meal, 3 parts -8 parts of molasses, 0.5 part -1.5 parts of multidimensional, 0.5 part of more mines -
1.5 parts, 1 part -5 parts of astragalus polyose；Fermentation packet includes following components according to mass parts：75 parts -85 parts of lactic acid bacteria, bacillus 5
Part -15 parts, 5 parts -15 parts of saccharomycete.
Further, the lactic acid bacteria is Pediococcus acidilactici or/and Lactobacillus casei.
The invention also discloses a kind of preparation methods of above-mentioned shrimp ferment, include the following steps：
Step 1 prepares fermentation packet；
Step 2 prepares solid medium；
Step 3, by the solid medium being prepared and fermentation packet according to mass ratio be 9:1 is mixed, and is added appropriate
Sterile water stirs evenly, and the mixed liquor of solid medium and fermentation packet is prepared；
Step 4, the commodity bag that the mixed liquor of solid medium and fermentation packet is sub-packed in the double-deck inner bag, are sealed, move
Enter thermostatic control room, under 28-32 DEG C of environment, stands 72 hours, carry out secondary fermentation, shrimp ferment is prepared.
Further, fermentation packet is prepared in the step 1 is specially：
Step 1.1 weighs：Following components is weighed according to mass parts：75 parts -85 parts of lactic acid bacteria, 5 parts -15 parts of bacillus,
5 parts -15 parts of saccharomycete；
It is prepared by step 1.2, leavening：Extracting lactic acid bacterium, bacillus and yeast seeds carry out seed culture respectively, prepare
Obtain lactic acid bacteria seed culture fluid, bacillus seed culture fluid and saccharomycete seed culture fluid；
Step 1.3, using staged fermentation：Bacillus seed culture fluid and saccharomycete seed culture fluid are added to fermentation
Aerobic fermentation is carried out in tank, and fermentation is terminated when OD values are more than or equal to 0.5；Lactic acid bacteria seed culture fluid is added into fermentation tank again
Anaerobic fermentation is carried out, 32-38 DEG C of temperature is controlled, 12~24 hours, fermentation, system is terminated when 0.6 and pH is less than or equal to 4 when OD is more than
It is standby to obtain mixed fermentation liquid；
Step 1.4, mixing：Mixed fermentation liquid is contained with clean container, brown sugar, the matter of brown sugar and mixed fermentation liquid is added
Amount is than being 4:1, mixing is sealed, fermentation packet is prepared, it is spare.
Further, the aerobic fermentation temperature of the bacillus seed culture fluid and saccharomycete seed culture fluid is 25-
35 DEG C, the aerobic fermentation time is 10~24 hours；The anaerobic fermentation temperature of lactic acid bacteria seed culture fluid is 32-38 DEG C, anaerobism hair
The ferment time is 12~24 hours.
Further, preparing solid medium in the step 2 is specially：
Dregs of beans, corn, wheat-middlings, peanut meal, fish meal, molasses, multidimensional, more mines and astragalus polyose are crossed 80 mesh by step 2.1
Sieve；
Step 2.2 weighs：Following components is weighed according to mass parts：25 parts -35 parts of dregs of beans, 10 parts -20 parts of corn, wheat-middlings
10 parts -20 parts, 5 parts -15 parts of peanut meal, 5 parts -15 parts of fish meal, 3 parts -8 parts of molasses, 0.5 part -1.5 parts of multidimensional, 0.5 part of more mines -
1.5 parts, 1 part -5 parts of astragalus polyose；
Load weighted each component is uniformly mixed by step 2.3 in stirring mixer, and solid medium is prepared, standby
With.
The invention also discloses a kind of application methods of above-mentioned shrimp ferment, include the following steps：Shrimp in 5cm or less,
According to mass ratio it is 1 by commercially available shrimp mixed feed and shrimp ferment:1 ratio mixing is used cooperatively, and shrimp, will in 5~10cm
Commercially available shrimp mixed feed and shrimp ferment are 7 according to mass ratio:3 ratio mixing is used cooperatively, and shrimp is in 10cm or more, by city
The shrimp mixed feed and shrimp ferment sold are 8 according to mass ratio:2 ratio mixing is used cooperatively.
Further, the shrimp mixed feed is commercially available litopenaeus vannamei compound feed.
Compared with prior art, the present invention can be obtained including following technique effect：
1) present invention is by fermentation technique, is that small peptide, short chain amino acid etc. are small by fermentative degradation macro-molecular protein
Molecular substance, more conducively shrimp are digested and assimilated, and protein utilization rate and conversion ratio are improved, and reduce the accumulation of harmful nitrogen class；Simultaneously to supporting
The stabilization and shrimp vigor for growing water body all have positive effect.
2) the composite fermentation packet used in the present invention, is combined, quantity is taken by lactic acid bacteria, bacillus and saccharomycete
With reasonable, function and effect mutually cooperate with, and mutually promote.
3) the characteristics of ferment of the present invention, proportioning is appropriate, has nutritional ingredient comprehensive, and food calling power is strong, water transfer steady water, promotes shrimp
Fast-growth, reduce feed coefficient, also just reduce aquaculture cost, improve the immunity of shrimp, enhance the disease resistance of shrimp, adjust
Breeding water body stability is saved, cultivation risk is reduced, improves cultivation success rate.
Certainly, it implements any of the products of the present invention it is not absolutely required to while reaching all the above technique effect.
Description of the drawings
Attached drawing described herein is used to provide further understanding of the present invention, and constitutes the part of the present invention, this hair
Bright illustrative embodiments and their description are not constituted improper limitations of the present invention for explaining the present invention.In the accompanying drawings：
Fig. 1 is the Penaeus Vannmei pond that the present invention uses ferment and unused ferment such as the eastern hut pattern entire culture-cycle
Pool pH situations of change；
Fig. 2 is the Penaeus Vannmei pond that the present invention uses ferment and unused ferment such as the eastern hut pattern entire culture-cycle
Pool ammonia nitrogen situation of change；
Fig. 3 is the Penaeus Vannmei pond that the present invention uses ferment and unused ferment such as the eastern hut pattern entire culture-cycle
Pool nitrite nitrogen situation of change.
Specific implementation mode
Carry out the embodiment that the present invention will be described in detail below in conjunction with embodiment, thereby to the present invention how application technology hand
Section solves technical problem and reaches the realization process of technical effect to fully understand and implement.
The invention discloses a seed shrimp ferment, including mass ratio is 9:1 solid medium and fermentation are wrapped, solid medium
Include following components according to mass parts：25 parts -35 parts of dregs of beans, 10 parts -20 parts of corn, 10 parts -20 parts of wheat-middlings, 5 part -15 of peanut meal
Part, 5 parts -15 parts of fish meal, 3 parts -8 parts of molasses, 0.5 part -1.5 parts of multidimensional, 0.5 part -1.5 parts of more mines, 1 part -5 parts of astragalus polyose；
Fermentation packet includes following components according to mass parts：75 parts -85 parts of lactic acid bacteria, 5 parts -15 parts of bacillus, 5 parts -15 parts of saccharomycete.
Ferment of the present invention has special aromatic odor, shrimps can be promoted to ingest, shrimps food ration is made to increase, and promotes
Shrimps are grown.
Ferment of the present invention is fermented by three kinds of beneficial bacteriums are reasonably combined, makes the high molecular weight protein in feed, be degraded into short
Chain amino acid, small peptide, it is easier to promote digesting and assimilating for shrimps.
Ferment of the present invention, used beneficial bacterium can be colonized in shrimps enteron aisle, and lactic acid bacteria is colonized in enteron aisle, be generated
Lactic acid makes intestinal pH be maintained at 4.8 or so, inhibits harmful microbe growth and breeding.Bacillus can secrete a variety of extracellular
Enzyme promotes digesting and assimilating for shrimp.
Ferment of the present invention, by adding multivitamin, several mineral materials, the increasings such as antibacterial peptide also generated through everfermentation
Strong immunity and oxidation resistant substance enhance the immunity and oxidation resistance of shrimp, enhance survival rate.
Ferment of the present invention can play the role of adding organic fertilizer into water body in the case of the residual bait that shrimp does not ingest.
Ferment of the present invention can play the role of adding beneficial bacterium into water body in the case of the residual bait that shrimp does not ingest,
Bacterium in regulation and control water body is mutually stablized, and is continuous a small amount of repeatedly addition, avoids causing stress reaction to shrimps.
The lactic acid bacteria is Pediococcus acidilactici or/and Lactobacillus casei.
The invention also discloses the preparation methods of a seed shrimp ferment, include the following steps：
Step 1 prepares fermentation packet：
Step 1.1 weighs：Following components is weighed according to mass parts：75 parts -85 parts of lactic acid bacteria, 5 parts -15 parts of bacillus,
5 parts -15 parts of saccharomycete；
It is prepared by step 1.2, leavening：Extracting lactic acid bacterium, bacillus and yeast seeds carry out seed culture respectively, prepare
Obtain lactic acid bacteria seed culture fluid, bacillus seed culture fluid and saccharomycete seed culture fluid；
Step 1.3, using staged fermentation：Bacillus seed culture fluid and saccharomycete seed culture fluid are added to fermentation
Aerobic fermentation is carried out in tank, controls 25-35 DEG C of temperature, 10~24 hours, fermentation is terminated when OD values, which are more than, waits 0.5；Again to hair
Addition lactic acid bacteria seed culture fluid progress anaerobic fermentation in fermentation tank, 32-38 DEG C of temperature of control, 12~24 hours, when OD is more than 0.6
Fermentation is terminated when being less than or equal to 4 with pH, mixed fermentation liquid is prepared；
Step 1.4, mixing：Mixed fermentation liquid is contained with clean container, brown sugar, the matter of brown sugar and mixed fermentation liquid is added
Amount is than being 4:1, mixing is sealed, fermentation packet is prepared, it is spare；
Step 2 prepares solid medium：
Dregs of beans, corn, wheat-middlings, peanut meal, fish meal, molasses, multidimensional, more mines and astragalus polyose are crossed 80 mesh by step 2.1
Sieve；The sieve mesh of the present invention is 80 mesh, keeps the big small particle of culture medium optimal, is applied to after breeding water body, shrimps can be better
It ingests, and cannot be easily caused bottom precipitation and destroy substrate.
Step 2.2 weighs：Following components is weighed according to mass parts：25 parts -35 parts of dregs of beans, 10 parts -20 parts of corn, wheat-middlings
10 parts -20 parts, 5 parts -15 parts of peanut meal, 5 parts -15 parts of fish meal, 3 parts -8 parts of molasses, 0.5 part -1.5 parts of multidimensional, 0.5 part of more mines -
1.5 parts, 1 part -5 parts of astragalus polyose；
Load weighted each component is uniformly mixed by step 2.3 in stirring mixer, and solid medium is prepared, standby
With；
Step 3, by the solid medium being prepared and fermentation packet according to mass ratio be 9:1 is mixed, and is added appropriate
Sterile water stirs evenly；
Step 4, the commodity bag for being sub-packed in the double-deck inner bag, are sealed, and move into thermostatic control room, under 28-32 DEG C of environment,
72 hours are stood, secondary fermentation is carried out；After fermentation, tunning is detected according to national standard microorganism detection method, measures institute
It is 3.8 × 10 to obtain fermentative feedstuff of microbe content of lactic acid bacteria8Cfu/g~4.1 × 109Cfu/g, bacillus content be 4.1 ×
108Cfu/g~5.5 × 109Cfu/g, yeast bacterial content are 5.1 × 108Cfu/g~4.0 × 109cfu/g。
The invention also discloses the application methods of a seed shrimp ferment, include the following steps：
For shrimp in 5cm or less, shrimp mixed feed (commercially available) and shrimp ferment are 1 according to mass ratio:1 ratio mixing cooperation makes
With, shrimp in 5~10cm, shrimp mixed feed (commercially available) and shrimp ferment are 7 according to mass ratio:3 ratio mixing is used cooperatively, shrimp
In 10cm or more, shrimp mixed feed (commercially available) and shrimp ferment are 8 according to mass ratio:2 ratio mixing is used cooperatively.
Embodiment 1
The preparation method of one seed shrimp ferment, includes the following steps：
Step 1 prepares fermentation packet：
Step 1.1 weighs：Following components is weighed according to mass parts：80 parts of lactic acid bacteria, 10 parts of bacillus, saccharomycete 10
Part；
It is prepared by step 1.2, leavening：Extracting lactic acid bacterium, bacillus and yeast seeds carry out seed culture respectively, prepare
Obtain lactic acid bacteria seed culture fluid, bacillus seed culture fluid and saccharomycete seed culture fluid；
Step 1.3, using staged fermentation：Bacillus seed culture fluid and saccharomycete seed culture fluid are added to fermentation
Aerobic fermentation is carried out in tank, controls 30 DEG C of temperature, 17 hours, fermentation is terminated when OD values, which are more than, waits 0.5；Add again into fermentation tank
Enter lactic acid bacteria seed culture fluid, controls 35 DEG C of temperature, 18 hours, fermentation, system are terminated when 0.6 and pH is less than or equal to 4 when OD is more than
It is standby to obtain mixed fermentation liquid；
Step 1.4 contains mixed fermentation liquid with clean container, and brown sugar, the mass ratio of brown sugar and mixed fermentation liquid is added
It is 4:1, mixing is sealed, fermentation packet is prepared, it is spare；
Step 2 prepares solid medium：
Dregs of beans, corn, wheat-middlings, peanut meal, fish meal, molasses, multidimensional, more mines and astragalus polyose are crossed 80 mesh by step 2.1
Sieve；
Step 2.2 weighs：Following components is weighed according to mass parts：30 parts of dregs of beans, 15 parts of corn, 15 parts of wheat-middlings, peanut meal
10 parts, 10 parts of fish meal, 5 parts of molasses, 1 part of multidimensional, 1 part of more mines, 3 parts of astragalus polyose；
Load weighted each component is uniformly mixed by step 2.3 in stirring mixer, and solid medium is prepared, standby
With；
Step 3, by the solid medium being prepared and fermentation packet according to mass ratio be 9:1 is mixed, and is added appropriate
Sterile water stirs evenly；
Step 4, the commodity bag for being sub-packed in the double-deck inner bag, are sealed, and move into thermostatic control room, quiet under 30 DEG C of environment
It sets 72 hours, carries out secondary fermentation；After fermentation, tunning is detected according to national standard microorganism detection method, measures gained
Fermentative feedstuff of microbe content of lactic acid bacteria is 1.2 × 109Cfu/g, bacillus content are 2.5 × 109Cfu/g, yeast bacterial content
It is 1.0 × 109cfu/g。
The application method of the shrimp ferment is as follows：The shrimp ferment applies the raising in Penaeus Vannmei, shrimp in 5cm or less,
Shrimp mixed feed (commercially available) and shrimp ferment are 1 according to mass ratio:1 ratio mixing is used cooperatively, and shrimp in 5~10cm, match by shrimp
It is 7 that feed (commercially available) and shrimp ferment, which are closed, according to mass ratio:3 ratio mixing is used cooperatively, and shrimp in 10cm or more, raise by shrimp cooperation
Expect that (commercially available) and shrimp ferment according to mass ratio are 8:2 ratio mixing is used cooperatively.
Embodiment 2
The preparation method of one seed shrimp ferment, includes the following steps：
Step 1 prepares fermentation packet：
Step 1.1 weighs：Following components is weighed according to mass parts：75 parts of lactic acid bacteria, 15 parts of bacillus, saccharomycete 10
Part；
It is prepared by step 1.2, leavening：Extracting lactic acid bacterium, bacillus and yeast seeds carry out seed culture respectively, prepare
Obtain lactic acid bacteria seed culture fluid, bacillus seed culture fluid and saccharomycete seed culture fluid；
Step 1.3, using staged fermentation：Bacillus seed culture fluid and saccharomycete seed culture fluid are added to fermentation
Aerobic fermentation is carried out in tank, controls 35 DEG C of temperature, 10 hours, fermentation is terminated when OD values, which are more than, waits 0.5；Add again into fermentation tank
Enter lactic acid bacteria seed culture fluid, controls 38 DEG C of temperature, 12 hours, fermentation, system are terminated when 0.6 and pH is less than or equal to 4 when OD is more than
It is standby to obtain mixed fermentation liquid；
Step 1.4 contains mixed fermentation liquid with clean container, and brown sugar, the mass ratio of brown sugar and mixed fermentation liquid is added
It is 4:1, mixing is sealed, fermentation packet is prepared, it is spare；
Step 2 prepares solid medium：
Dregs of beans, corn, wheat-middlings, peanut meal, fish meal, molasses, multidimensional, more mines and astragalus polyose are crossed 80 mesh by step 2.1
Sieve；
Step 2.2 weighs：Following components is weighed according to mass parts：25 parts of dregs of beans, 20 parts of corn, 10 parts of wheat-middlings, peanut meal
15 parts, 5 parts of fish meal, 8 parts of molasses, 0.5 part of multidimensional, 1.5 parts of more mines, 1 part of astragalus polyose；
Load weighted each component is uniformly mixed by step 2.3 in stirring mixer, and solid medium is prepared, standby
With；
Step 3, by the solid medium being prepared and fermentation packet according to mass ratio be 9:1 is mixed, and is added appropriate
Sterile water stirs evenly；
Step 4, the commodity bag for being sub-packed in the double-deck inner bag, are sealed, and move into thermostatic control room, quiet under 28 DEG C of environment
It sets 72 hours, carries out secondary fermentation；After fermentation, tunning is detected according to national standard microorganism detection method, measures gained
Fermentative feedstuff of microbe content of lactic acid bacteria is 3.8 × 108Cfu/g, bacillus content are 5.5 × 109Cfu/g, yeast bacterial content
It is 2.0 × 109cfu/g。
The application method of the shrimp ferment is as follows：The shrimp ferment applies the raising in Penaeus Vannmei, shrimp in 5cm or less,
Shrimp mixed feed (commercially available) and shrimp ferment are 1 according to mass ratio:1 ratio mixing is used cooperatively, and shrimp in 5~10cm, match by shrimp
It is 7 that feed (commercially available) and shrimp ferment, which are closed, according to mass ratio:3 ratio mixing is used cooperatively, and shrimp shrimp cooperation in 10cm or more is raised
Expect that (commercially available) and shrimp ferment according to mass ratio are 8:2 ratio mixing is used cooperatively.
Embodiment 3
The preparation method of one seed shrimp ferment, includes the following steps：
Step 1 prepares fermentation packet：
Step 1.1 weighs：Following components is weighed according to mass parts：80 parts of lactic acid bacteria, 5 parts of bacillus, saccharomycete 15
Part；
It is prepared by step 1.2, leavening：Extracting lactic acid bacterium, bacillus and yeast seeds carry out seed culture respectively, prepare
Obtain lactic acid bacteria seed culture fluid, bacillus seed culture fluid and saccharomycete seed culture fluid；
Step 1.3, using staged fermentation：Bacillus seed culture fluid and saccharomycete seed culture fluid are added to fermentation
Aerobic fermentation is carried out in tank, controls 25 DEG C of temperature, 24 hours, fermentation is terminated when OD values, which are more than, waits 0.5；Add again into fermentation tank
Enter lactic acid bacteria seed culture fluid, controls 32 DEG C of temperature, 24 hours, fermentation, system are terminated when 0.6 and pH is less than or equal to 4 when OD is more than
It is standby to obtain mixed fermentation liquid；
Step 1.4 contains mixed fermentation liquid with clean container, and brown sugar, the mass ratio of brown sugar and mixed fermentation liquid is added
It is 4:1, mixing is sealed, fermentation packet is prepared, it is spare；
Step 2 prepares solid medium：
Dregs of beans, corn, wheat-middlings, peanut meal, fish meal, molasses, multidimensional, more mines and astragalus polyose are crossed 80 mesh by step 2.1
Sieve；
Step 2.2 weighs：Following components is weighed according to mass parts：35 parts of dregs of beans, 10 parts of corn, 20 parts of wheat-middlings, peanut meal
5 parts, 15 parts of fish meal, 3 parts of molasses, 1.5 parts of multidimensional, 0.5 part of more mines, 5 parts of astragalus polyose；
Load weighted each component is uniformly mixed by step 2.3 in stirring mixer, and solid medium is prepared, standby
With；
Step 3, by the solid medium being prepared and fermentation packet according to mass ratio be 9:1 is mixed, and is added appropriate
Sterile water stirs evenly；
Step 4, the commodity bag for being sub-packed in the double-deck inner bag, are sealed, and move into thermostatic control room, quiet under 32 DEG C of environment
It sets 72 hours, carries out secondary fermentation；After fermentation, tunning is detected according to national standard microorganism detection method, measures gained
Fermentative feedstuff of microbe content of lactic acid bacteria is 3.1 × 109Cfu/g, bacillus content are 4.1 × 108Cfu/g, yeast bacterial content
It is 4.0 × 109cfu/g。。
The application method of the shrimp ferment is as follows：The shrimp ferment applies the raising in Macrobrachium rosenbergii, and shrimp is in 5cm or less, shrimp
Mixed feed (commercially available) and shrimp ferment are 1 according to mass ratio:1 ratio mixing is used cooperatively, and shrimp is in 5~10cm, shrimp cooperation
Feed (commercially available) and shrimp ferment are 7 according to mass ratio:3 ratio mixing is used cooperatively, and shrimp is in 10cm or more, shrimp mixed feed
(commercially available) and shrimp ferment are 8 according to mass ratio:2 ratio mixing is used cooperatively.
Embodiment 4
The preparation method of one seed shrimp ferment, includes the following steps：
Step 1 prepares fermentation packet：
Step 1.1 weighs：Following components is weighed according to mass parts：85 parts of lactic acid bacteria, 10 parts of bacillus, saccharomycete 5
Part；
It is prepared by step 1.2, leavening：Extracting lactic acid bacterium, bacillus and yeast seeds carry out seed culture respectively, prepare
Obtain lactic acid bacteria seed culture fluid, bacillus seed culture fluid and saccharomycete seed culture fluid；
Step 1.3, using staged fermentation：Bacillus seed culture fluid and saccharomycete seed culture fluid are added to fermentation
Aerobic fermentation is carried out in tank, controls 28 DEG C of temperature, 20 hours, fermentation is terminated when OD values, which are more than, waits 0.5；Add again into fermentation tank
Enter lactic acid bacteria seed culture fluid carry out anaerobic fermentation, control 36 DEG C of temperature, 18 hours, when OD be more than 0.6 and pH be less than or equal to 4 when
Fermentation is terminated, mixed fermentation liquid is prepared；
Step 1.4, mixing：Mixed fermentation liquid is contained with clean container, brown sugar, the matter of brown sugar and mixed fermentation liquid is added
Amount is than being 4:1, mixing is sealed, fermentation packet is prepared, it is spare；
Step 2 prepares solid medium：
Dregs of beans, corn, wheat-middlings, peanut meal, fish meal, molasses, multidimensional, more mines and astragalus polyose are crossed 80 mesh by step 2.1
Sieve；The sieve mesh of the present invention is 80 mesh, keeps the big small particle of culture medium optimal, is applied to after breeding water body, shrimps can be better
It ingests, and cannot be easily caused bottom precipitation and destroy substrate.
Step 2.2 weighs：Following components is weighed according to mass parts：35 parts of dregs of beans, 10 parts of corn, 20 parts of wheat-middlings, peanut meal
5 parts, 15 parts of fish meal, 3 parts of molasses, 1.5 parts of multidimensional, 0.5 part of more mines, 5 parts of astragalus polyose；
Load weighted each component is uniformly mixed by step 2.3 in stirring mixer, and solid medium is prepared, standby
With；
Step 3, by the solid medium being prepared and fermentation packet according to mass ratio be 9:1 is mixed, and is added appropriate
Sterile water stirs evenly；
Step 4, the commodity bag for being sub-packed in the double-deck inner bag, are sealed, and move into thermostatic control room, quiet under 28 DEG C of environment
It sets 72 hours, carries out secondary fermentation；After fermentation, tunning is detected according to national standard microorganism detection method, measures gained
Fermentative feedstuff of microbe content of lactic acid bacteria is 4.1 × 109Cfu/g, bacillus content are 4.1 × 108Cfu/g, yeast bacterial content
It is 4.0 × 109cfu/g。
The invention also discloses the application methods of a seed shrimp ferment, include the following steps：
For shrimp in 5cm or less, shrimp mixed feed (commercially available) and shrimp ferment are 1 according to mass ratio:1 ratio mixing cooperation makes
With, shrimp in 5~10cm, shrimp mixed feed (commercially available) and shrimp ferment are 7 according to mass ratio:3 ratio mixing is used cooperatively, shrimp
In 10cm or more, shrimp mixed feed (commercially available) and shrimp ferment are 8 according to mass ratio:2 ratio mixing is used cooperatively.It ties below
Specific experimental data is closed to illustrate the technique effect of the present invention：
1, the Penaeus Vannmei hut aquaculture model of Jiangsu Rudong, concrete outcome are shown in Table 1 and Fig. 1-Fig. 3.
1 hut pattern of table is using ferment and does not use ferment comparative situation
Wherein, A groups are shrimp mixed feed (commercially available)；B groups are ferment of the present invention (formula of embodiment 1)+shrimp mixed feed (city
It sells).
It can be obtained from Fig. 1-Fig. 3, it is identical in other factors, ferment using ferment and is not used by comparison
Element, will not be significantly raised using cultivating pool pH, ammonia nitrogen and nitrite nitrogen the cultivation later stage of ferment, so that it is exceeded, to supporting
It grows shrimps and generates toxic action, after ferment, pH, ammonia nitrogen and nitrite nitrogen, control in the reasonable scope, generate shrimps
Smaller murder by poisoning promotes cultivation success rate.
2, Xiaoshan, Zhejiang Penaeus Vannmei greenhouse aquaculture model, concrete outcome are shown in Table 2.
2 greenhouse pattern of table is using ferment and does not use ferment comparative situation
Wherein, A groups are shrimp mixed feed (commercially available)；B groups are ferment of the present invention (formula of embodiment 2)+shrimp mixed feed (city
It sells).
3, Macrobrachium rosenbergii pond culture pattern, concrete outcome are shown in Table 3.
3 pond culture pattern of table is using ferment and does not use ferment comparative situation
Wherein, A groups are shrimp mixed feed (commercially available)；B groups are ferment of the present invention (formula of embodiment 3)+shrimp mixed feed (city
It sells).
From table 1- tables 3 as can be seen that the ferment that the present invention is prepared can make the food ration of shrimps, the speed of growth, resist
Sick life ability, water column stability, yield and economic benefit, which have, to be obviously improved.
In conclusion the shrimp ferment of the present invention has the following advantages：
1) improve the quality of feed
1.1) present invention eliminates the anti-nutritional factors in feed, such as protease inhibitors, phytic acid through everfermentation.
1.2) macromolecules degradation that the present invention makes carbohydrate, protein in feed etc. complicated through everfermentation is the list easily absorbed
The small molecules such as sugar, oligosaccharide and amino acid.
1.3) present invention will produce microbial bacteria body protein full of nutrition and useful metabolite through everfermentation, such as
Amino acid, organic acid, antibacterial peptide etc..
1.4) present invention generates the organic acids such as lactic acid by lactic acid bacteria class fermentation, can increase gastrointestinal tract acidity, enhances stomach and intestine
The digested enzymatic activity in road improves digestion power.
1.5) content of the invention through vibrios in feed, toxin (aflatoxin etc.) can be reduced after everfermentation.
2) improve the health status of animal body
2.1) a variety of digestive ferments such as protease, the lipase that fermented feed of the present invention will produce promote cultivated animals digestion
It absorbs.
2.2) beneficial microbe can generate a variety of B family vitamins in enteron aisle intracellular metabolite in fermented feed of the present invention, and enhancing is supported
Grow animal vigor.
2.3) present invention maintains microecological balance in animal body enteron aisle, and cultivated animals ingest after fermented feed, thalline and its
Metabolite can promote beneficial bacterium in cultivated animals enteron aisle (lactic acid bacteria, Bifidobacterium) to be proliferated, and increase the quantity of intestinal beneficial bacterium,
Improve intestine microenvironment.
3) enhance immunity of organisms
3.1) contain a large amount of superoxide dismutase in viable lactic acid bacteria liquid and its metabolite in fermented feed of the present invention
Enzyme can improve the immunity of cultivated animals.
3.2) astragalus polyose, multiple-microorganism added in fermented feed of the present invention etc. enhances cultivated animals immunity.
4) diseases prevention
Lactic acid bacteria can effectively reduce the quantity of pathogenic microorganism in animal intestinal tract in fermented feed of the present invention, be risen to pathogenic bacteria
To Ant agonism, and then achieve the purpose that diseases prevention.
5) improve feeding environment, stablize breeding water body
5.1) generation for reducing the harmful substances such as cultivated animals enteron aisle biogenic amine, ammonia, indoles, to reduce cultivated animals
Drain NH3、H2The quantity of S reduces pollution, improves feeding environment.
5.1) fermented feed is stablized reducing the content of invalid albumen and into water body by way of continuous conveying beneficial bacterium
Water body beneficial microorganism quantity, ammonia nitrogen, nitrite nitrogen in water body of degrading, avoids answering for interim generation of disposably splashing
Swash.
5.2) fermented feed ensures the continuous growth and breeding of beneficial microorganism in water body by regulating and controlling carbon-nitrogen ratio in water body.
5.3) by adding a variety of amino acid, several mineral materials, multivitamin in fermented feed, and regulate and control N/P ratio
Promote the stabilization growth and breeding of beneficial algae, ensures that water body is stablized.
Above description has shown and described several preferred embodiments of invention, but as previously described, it should be understood that invention is not
It is confined to form disclosed herein, is not to be taken as excluding other embodiments, and can be used for various other combinations, modification
And environment, and can be carried out by the above teachings or related fields of technology or knowledge in the scope of the invention is set forth herein
Change.And changes and modifications made by those skilled in the art do not depart from the spirit and scope of invention, then should all be weighed appended by invention
In the protection domain that profit requires.
Claims (8)
1. a seed shrimp ferment, which is characterized in that including mass ratio be 9:1 solid medium and fermentation are wrapped, and solid medium is pressed
Include following components according to mass parts：25 parts -35 parts of dregs of beans, 10 parts -20 parts of corn, 10 parts -20 parts of wheat-middlings, 5 part -15 of peanut meal
Part, 5 parts -15 parts of fish meal, 3 parts -8 parts of molasses, 0.5 part -1.5 parts of multidimensional, 0.5 part -1.5 parts of more mines, 1 part -5 parts of astragalus polyose；
Fermentation packet includes following components according to mass parts：75 parts -85 parts of lactic acid bacteria, 5 parts -15 parts of bacillus, 5 parts -15 parts of saccharomycete.
2. shrimp ferment according to claim 1, which is characterized in that the lactic acid bacteria is Pediococcus acidilactici or/and cheese breast
Bacillus.
3. a kind of preparation method of shrimp ferment described in claim 1, which is characterized in that include the following steps：
Step 1 prepares fermentation packet；
Step 2 prepares solid medium；
Step 3, by the solid medium being prepared and fermentation packet according to mass ratio be 9:1 is mixed, and is added appropriate sterile
Water stirs evenly, and the mixed liquor of solid medium and fermentation packet is prepared；
Step 4, the commodity bag that the mixed liquor of solid medium and fermentation packet is sub-packed in the double-deck inner bag, are sealed, move into permanent
Warm control room stands 72 hours under 28-32 DEG C of environment, carries out secondary fermentation, shrimp ferment is prepared.
4. preparation method according to claim 3, which is characterized in that preparing fermentation packet in the step 1 is specially：
Step 1.1 weighs：Following components is weighed according to mass parts：75-85 parts of lactic acid bacteria, 5 parts -15 parts of bacillus, saccharomycete
5 parts -15 parts；
It is prepared by step 1.2, leavening：Extracting lactic acid bacterium, bacillus and yeast seeds carry out seed culture respectively, are prepared
Lactic acid bacteria seed culture fluid, bacillus seed culture fluid and saccharomycete seed culture fluid；
Step 1.3, using staged fermentation：Bacillus seed culture fluid and saccharomycete seed culture fluid are added in fermentation tank
Aerobic fermentation is carried out, fermentation is terminated when OD values are more than or equal to 0.5；Lactic acid bacteria seed culture fluid is added into fermentation tank again to carry out
Anaerobic fermentation terminates fermentation when OD is more than when 0.6 and pH is less than or equal to 4, mixed fermentation liquid is prepared；
Step 1.4, mixing：Mixed fermentation liquid is contained with clean container, brown sugar, the mass ratio of brown sugar and mixed fermentation liquid is added
It is 4:1, mixing is sealed, fermentation packet is prepared, it is spare.
5. preparation method according to claim 4, which is characterized in that the bacillus seed culture fluid and barms
The aerobic fermentation temperature of sub- culture solution is 25-35 DEG C, and the aerobic fermentation time is 10~24 hours；Lactic acid bacteria seed culture fluid is detested
Aerobe fermentation temperature is 32-38 DEG C, and the anaerobic fermentation time is 12~24 hours.
6. preparation method according to claim 3, which is characterized in that preparing solid medium in the step 2 is specially：
Dregs of beans, corn, wheat-middlings, peanut meal, fish meal, molasses, multidimensional, more mines and astragalus polyose are crossed 80 mesh sieve by step 2.1；
Step 2.2 weighs：Following components is weighed according to mass parts：25 parts -35 parts of dregs of beans, 10 parts -20 parts of corn, 10 parts of wheat-middlings -
20 parts, 5 parts -15 parts of peanut meal, 5 parts -15 parts of fish meal, 3 parts -8 parts of molasses, 0.5 part -1.5 parts of multidimensional, 0.5 part -1.5 parts of more mines,
1 part -5 parts of astragalus polyose；
Load weighted each component is uniformly mixed by step 2.3 in stirring mixer, and solid medium is prepared, spare.
7. described in the shrimp ferment and claim 3-6 any claims described in a kind of claim 1-2 any claims
The application method of shrimp ferment that is prepared of preparation method, which is characterized in that include the following steps：Shrimp, will in 5cm or less
Commercially available shrimp mixed feed and shrimp ferment are 1 according to mass ratio:1 ratio mixing is used cooperatively, and shrimp is in 5~10cm, by city
The shrimp mixed feed and shrimp ferment sold are 7 according to mass ratio:3 ratio mixing is used cooperatively, and shrimp, will be commercially available in 10cm or more
Shrimp mixed feed and shrimp ferment according to mass ratio be 8:2 ratio mixing is used cooperatively.
8. application method according to claim 7, which is characterized in that the shrimp mixed feed is commercially available Penaeus Vannmei
Mixed feed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810184637.9A CN108402337A (en) | 2018-03-07 | 2018-03-07 | One seed shrimp ferment and the preparation method and application thereof |
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| CN201810184637.9A CN108402337A (en) | 2018-03-07 | 2018-03-07 | One seed shrimp ferment and the preparation method and application thereof |
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| CN112075368A (en) * | 2020-09-23 | 2020-12-15 | 江苏宝源生物科技有限公司 | Prawn digestive tract thickening method |
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Application publication date: 20180817 |