CN107518213A - A kind of biological agent for preventing Macrobrachium nipponensis Yearling and preparation method thereof - Google Patents
A kind of biological agent for preventing Macrobrachium nipponensis Yearling and preparation method thereof Download PDFInfo
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- CN107518213A CN107518213A CN201710807362.5A CN201710807362A CN107518213A CN 107518213 A CN107518213 A CN 107518213A CN 201710807362 A CN201710807362 A CN 201710807362A CN 107518213 A CN107518213 A CN 107518213A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
- A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
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- A23K20/22—Compounds of alkali metals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of biological agent for preventing Macrobrachium nipponensis Yearling, by base-material and saccharomyces cerevisiae bacterium solution, lactobacterium acidophilus' liquid, bacillus licheniformis liquid and water by weight 100：3~4：3~4：5~7：35~45 mixing after solid state fermentation and obtain；Wherein, by weight percentage, the base-material is made up of 50~70wt% of soybean meal, 4~5wt% of rapeseed dregs, 10~15wt% of fish meal, 5~8wt% of the vinasse dregs of rice, 2~5wt% of cornstarch, 1~3wt% of wheat bran, 1~3wt% of molasses, 1~3wt% of bloom and 0.5~1wt% of sodium carbonate.The biological agent of the obtained prevention Macrobrachium nipponensis Yearling of the inventive method, solving the problems, such as that Macrobrachium nipponensis caused by sex premature is caused on gonad development is slow-growing influences listing specification；Also there is coordinating intestines and stomach, increase beneficial bacterium quantity simultaneously, purify water, improve immunity.
Description
Technical field
The invention belongs to agro-ecology product technique field, and in particular to a kind of biology for preventing Macrobrachium nipponensis Yearling
Preparation and preparation method thereof.
Background technology
Macrobrachium nipponensis (Macrobrachium nipponense) is commonly called as river prawn, freshwater shrimp, is under the jurisdiction of Malacostraca, full
Mesh, Natantia, Palaemonidae, pond crayfish category, Japan and China are distributed mainly on, are that China's cultured area is most wide, yield is maximum
One of economic freshwater shrimps.It grows soon, and individual is big, and growth cycle is short, and breeding is fast, and vitality is strong, strong adaptability, feeding habits
Extensively, meat flavour is delicious, and the advantages that can listing throughout the year, was distributed widely in China's freshwater lake.
Macrobrachium nipponensis aquaculture starts to walk since the beginning of the nineties in last century, because Macrobrachium nipponensis cultivation has market prospects
The advantages of good, technical operation is simply, startup investment and production cost is relatively low, still, as Macrobrachium nipponensis cultivates the continuous of scale
Expanding, the rapid development of production, Macrobrachium nipponensis aquaculture is there is also problems, as cultural technique level is uneven, cultivation
Technology is lack of standardization, and freshwater shrimp germplasm is degenerated, and sexal maturity is done sth. in advance, and disease increases, and pattern is single etc., causes freshwater shrimp commodity rate low, product
Position declines, and the market competitiveness is not strong, has a strong impact on the sound development of shrimp culture.
Macrobrachium nipponensis sexal maturity shifts to an earlier date, and individual generally diminishes, and commodity rate declines, and averagely only 50% or so.Yearling
Growth period is caused to shorten, autumn excessively breeds, and pond density is uncontrollable, and cultivation individual is uneven.Cause sexual gland maturation
Reason has：1) local seed shrimp is selected to be easily caused Macrobrachium nipponensis as parent, inbred；2) Antibiogics usage in breeding process
Too much；3) when hatching shrimp seedling, some nursery producers covet high benefit and shortening zoea metamorphosis time；4) it is long-term
Intake high protein feed causes dysbolism, physiological function imbalance, and constitution declines, and causes disease；5) the freshwater shrimp growth of sex premature
Slowly, for nutrient accumulation on gonad development, commercial sized prawn specification is less than normal, and listed price is low, directly affects economic benefit.
The japonicus amount reproduction son shrimp of sex premature, makes freshwater shrimp density in pond increase rapidly, young shrimp and strives food into shrimp, strives
Oxygen, space is striven, cause into shrimp specification and do not increase, commodity price is greatly lowered, and have impact on cultured output again, so as to constrain Japan
The sound development of pond crayfish aquaculture.
In addition, Dysecdysis disease, during adult shrimp breeding, it sometimes appear that the phenomenon that shrimp can not cast off a skin for a long time, just not
The freshwater shrimp color of husking is brown, matt, there is a sensation of double shells, and its reason is probably trophic disturbance, or environmental degradation and
Disease infects.The physiological structure of aquatic livestock is different from nonruminant and the ruminants such as livestock and poultry, thus aquatic livestock is to nutrition
The demand of material, digestion, absorption and utilize etc. and to have its particularity, with regard to Macrobrachium nipponensis major embodiment in the following areas：
1) Macrobrachium nipponensis is more much higher than livestock and poultry to the demand of feed protein, and protein content typically exists in livestock and poultry diet
Less than 20%, and Macrobrachium nipponensis volume protein level is then more than 40%；
2) the alimentary canal differentiation of Macrobrachium nipponensis is simple, and alimentary canal is shorter, only 1/1 to five/3rds food of livestock and poultry
Thing residence time in alimentary canal is short, and glandula digestive is also undeveloped, and for digestive ferment because body temperature is low, activity is not also high；
3) play that the bacterial species of digestion are few, and quantity is also few in Macrobrachium nipponensis enteron aisle；
4) Macrobrachium nipponensis amylase activity is very weak, it is impossible to by the use of carbohydrate as the main energy sources of body, and can only
Using the protein in feed as the energy, the energy is provided for statistics growth by a large amount of amino-oxide groups acid.
In summary, easy to digest for Macrobrachium nipponensis raising, the protein raw material for absorbing and utilizing is feed in breeding process
The core content of nutrition.Meanwhile feed on the market generally with the addition of hormone, antibiotic etc, and formed to shrimp body secondary
Pollution, bigger injury is brought to consumer.
The content of the invention
The present invention proposes a kind of biology system for preventing Macrobrachium nipponensis Yearling to solve above mentioned problem of the prior art
Agent and preparation method thereof.
The biological agent of prevention Macrobrachium nipponensis Yearling provided by the present invention, its be it is a kind of by protein raw material with it is auxiliary
Expect the batch mixing mixed, sterilized by thermophilic digestion, the nonreactive then obtained by compound probiotic kind microorganism solid fermentation
The oxygen factor, contain small peptide additive for enriching prebiotic small peptide and the prebiotic factor and preparation method thereof
To achieve the above object, the present invention uses following technical scheme：
The first aspect of the invention is to provide a kind of biological agent for preventing Macrobrachium nipponensis Yearling, by base-material with making
Brewer yeast bacterium solution, lactobacterium acidophilus' liquid, bacillus licheniformis liquid and water are by weight 100：3~4：3~4：5~7：35~45 is mixed
Solid state fermentation after conjunction and obtain；Wherein, by weight percentage, the base-material by 50~70wt% of soybean meal, rapeseed dregs 4~
5wt%, 10~15wt% of fish meal, 5~8wt% of the vinasse dregs of rice, 2~5wt% of cornstarch, 1~3wt% of wheat bran, molasses 1~
3wt%, 1~3wt% of bloom and 0.5~1wt% of sodium carbonate compositions.
Further, the weight of the base-material and saccharomyces cerevisiae bacterium solution, lactobacterium acidophilus' liquid, bacillus licheniformis liquid and water
Than for 100：3.5~4：3~3.5：5.5~6.5：38~42.
Further, the base-material by 55~65wt% of soybean meal, 4.2~4.6wt% of rapeseed dregs, fish meal 12~
14wt%, 6~8wt% of the vinasse dregs of rice, 3~4wt% of cornstarch, 1.5~2wt% of wheat bran, 2~3wt% of molasses, bloom 1.5~
2wt% and 0.6~0.8wt% of sodium carbonate compositions.
The second aspect of the present invention is to provide a kind of preparation method for the biological agent for preventing Macrobrachium nipponensis Yearling, tool
Body comprises the following steps：
(1) by 50~70wt% of soybean meal, 4~5wt% of rapeseed dregs, 10~15wt% of fish meal, 5~8wt% of the vinasse dregs of rice,
2~5wt% of cornstarch, 1~3wt% of wheat bran, 1~3wt% of molasses, 1~3wt% of bloom and 0.5~1wt% of sodium carbonate are mixed
Close, obtain base-material, high-temperature sterilization；
(2) S. cervisiae, lactobacterium acidophilus and bacillus licheniformis are inoculated into respective culture medium respectively and carried out
Culture, obtains saccharomyces cerevisiae bacterium solution, lactobacterium acidophilus' liquid and bacillus licheniformis liquid；
(3) by the base-material of gained in step (1) and saccharomyces cerevisiae bacterium solution, lactobacterium acidophilus' liquid, bacillus licheniformis liquid and
Water is by weight 100：3~4：3~4：5~7：35~45 mixing, adjust pH to 4.5~5.0, in 30~40 DEG C of solid state fermentations 3
~5 days, gained tunning was dry, crushes, and the biological agent of prevention Macrobrachium nipponensis Yearling is made.
Further, the cultural method of saccharomyces cerevisiae bacterium solution is in the step (2)：By S. cervisiae by weight 2
~5：100 are inoculated into S. cervisiae special culture media, 29~35 DEG C of 10~26h of culture, obtain saccharomyces cerevisiae bacterium solution.
Further, the cultural method of lactobacterium acidophilus' liquid is in the step (2)：By lactobacterium acidophilus by weight 2
~5：100 are inoculated into lactobacterium acidophilus' culture medium, 33~37 DEG C of 9~25h of culture, obtain lactobacterium acidophilus' liquid.
It is further preferred that lactobacterium acidophilus' culture medium is by MRA culture mediums and inulin, whey powder, casein hydrolysis
Thing and carrot juice are by weight 100：0.1~1：0.1~1：0.1~1：1~10 is obtained by mixing.
Further, the cultural method of bacillus licheniformis liquid is in the step (2)：By bacillus licheniformis by weight
Than 2~5：100 are inoculated into LB culture mediums, 35~38 DEG C of 23~26h of culture, obtain bacillus licheniformis liquid.
The present invention uses above-mentioned technical proposal, compared with prior art, has the following technical effect that：
Using the biological agent for preventing Macrobrachium nipponensis Yearling made from the inventive method, small peptide therein is fully to close
Reason ground utilizes the protein in feed, solves because long-term intake high protein feed causes dysbolism, physiological function imbalance, constitution
Decline, and superfluous nutrient accumulation Macrobrachium nipponensis to caused by causing sex premature on gonad development is slow-growing, influences listing specification；
Also there is coordinating intestines and stomach, increase beneficial bacterium quantity simultaneously, purify water, improve immunity；Hormone, antibiotic are not added
Deng medicine, have and make cheap simple, component safety, cost, nutritious, compatibility science, the spy that attractant is good, quick
Point.
Embodiment
The present invention is described in more detail below by specific embodiment, for a better understanding of the present invention,
But following embodiments are not intended to limit the scope of the invention.
Embodiment 1 prevents the preparation of the biological agent of Macrobrachium nipponensis Yearling, specifically comprises the following steps：
(1) by soybean meal 70wt%, rapeseed dregs 5wt%, fish meal 14.5wt%, vinasse dregs of rice 5wt%, cornstarch
2wt%, wheat bran 1wt%, molasses 1wt%, bloom 1wt% and sodium carbonate 0.5wt% mixing, obtain base-material, 110 DEG C of thermophilic digestions
0.5h is sterilized；
(2) by S. cervisiae by weight 2：100 are inoculated into S. cervisiae special culture media, 35 DEG C of culture 10h, obtain
To saccharomyces cerevisiae bacterium solution；
By lactobacterium acidophilus by weight 2：100 are inoculated into lactobacterium acidophilus' culture medium, 37 DEG C of culture 25h, obtain thermophilic
Sour lactobacillus suspension, described lactobacillus acidophilus culture medium in MRA culture mediums by adding inulin (also known as synanthrin), whey powder, junket
Protolysate and carrot juice obtain, and described MRA culture mediums include the parts by weight of peptone 5, the parts by weight of meat medicinal extract 5, yeast extract
3 parts by weight, the parts by weight of glucose 15, the parts by weight of sodium acetate 3, the parts by weight of Tween 80 0.5, the parts by weight of Triammonium citrate 1.5, phosphoric acid
The parts by weight of hydrogen dipotassium 1.5, the parts by weight of magnesium sulfate 0.1, the parts by weight of manganese sulfate 0.2, the parts by weight of distilled water 1000, its pH value are 6.2-
6.4；The weight ratio of described MRA culture mediums and inulin, whey powder, casein hydrolysate and carrot juice is 100：0.5：0.5：
0.5：5.
By bacillus licheniformis by weight 2：100 are inoculated into LB culture mediums, 37 DEG C of culture 25h, obtain lichens gemma
Bacillus liquid.
(3) by the base-material of gained in the first step and saccharomyces cerevisiae bacterium solution, lactobacterium acidophilus' liquid, bacillus licheniformis liquid and water
By weight 100：3：3：5：35 mixing, adjust pH to 4.5, and in 30 DEG C of solid state fermentations 3 days, gained tunning used air-flow stream
Change that bed is dry, leaving air temp is 45 DEG C, EAT is 125 DEG C, is crushed, and crosses 1.0 eye mesh screens, and prevention Macrobrachium nipponensis sexual gland is made
Precocious biological agent；Small peptide total amount >=20%, crude protein content >=55% are determined, molecular weight is less than 20000Dalton, amino
Nitrogen is 0.8~1.0%, total nitrogen >=10%, lactic acid content >=3.0%, moisture≤10%.
Embodiment 2 prevents the preparation of the biological agent of Macrobrachium nipponensis Yearling, specifically comprises the following steps：
(1) by soybean meal 65wt%, rapeseed dregs 4wt%, fish meal 13wt%, vinasse dregs of rice 6wt%, cornstarch 5wt%,
Wheat bran 2wt%, molasses 2wt%, bloom 2wt% and sodium carbonate 1wt% mixing, obtain base-material, 105 DEG C of thermophilic digestion 1h sterilizations；
(2) by S. cervisiae by weight 3：100 are inoculated into S. cervisiae special culture media, 32 DEG C of culture 12h, obtain
To saccharomyces cerevisiae bacterium solution；
By lactobacterium acidophilus by weight 5：100 are inoculated into lactobacterium acidophilus' culture medium, 33 DEG C of culture 14h, obtain thermophilic
Sour lactobacillus suspension, described lactobacillus acidophilus culture medium in MRA culture mediums by adding inulin (also known as synanthrin), whey powder, junket
Protolysate and carrot juice obtain, and described MRA culture mediums include the parts by weight of peptone 5, the parts by weight of meat medicinal extract 5, yeast extract
3 parts by weight, the parts by weight of glucose 15, the parts by weight of sodium acetate 3, the parts by weight of Tween 80 0.5, the parts by weight of Triammonium citrate 1.5, phosphoric acid
The parts by weight of hydrogen dipotassium 1.5, the parts by weight of magnesium sulfate 0.1, the parts by weight of manganese sulfate 0.2, the parts by weight of distilled water 1000, its pH value are 6.2-
6.4；The weight ratio of described MRA culture mediums and inulin, whey powder, casein hydrolysate and carrot juice is 100：0.5：0.5：
0.5：5.
By bacillus licheniformis by weight 4：100 are inoculated into LB culture mediums, 37 DEG C of culture 25h, obtain lichens gemma
Bacillus liquid.
(3) by the base-material of gained in the first step and saccharomyces cerevisiae bacterium solution, lactobacterium acidophilus' liquid, bacillus licheniformis liquid and water
By weight 100：3.5：3：6.5：40 mixing, adjust pH to 4.5, and in 32 DEG C of solid state fermentations 3 days, gained tunning used gas
Stream fluidized bed drying, leaving air temp are 45 DEG C, and EAT is 125 DEG C, is crushed, and cross 1.0 eye mesh screens, obtained prevention Macrobrachium nipponensis
The biological agent of Yearling；Small peptide total amount >=25%, crude protein content >=61% are determined, molecular weight is less than 20000Dalton,
Ammonia nitrogen is 0.8~1.0%, total nitrogen >=10%, lactic acid content >=3.0%, moisture≤10%.
Embodiment 3 prevents the preparation of the biological agent of Macrobrachium nipponensis Yearling, specifically comprises the following steps：
(1) by soybean meal 60wt%, rapeseed dregs 5wt%, fish meal 15wt%, vinasse dregs of rice 7wt%, cornstarch 5wt%,
Wheat bran 2wt%, molasses 2wt%, bloom 3wt% and sodium carbonate 1wt% mixing, obtain base-material, 105 DEG C of thermophilic digestion 1h sterilizations；
(2) by S. cervisiae by weight 4：100 are inoculated into S. cervisiae special culture media, 29 DEG C of culture 26h, obtain
To saccharomyces cerevisiae bacterium solution.
By lactobacterium acidophilus by weight 1：100 are inoculated into lactobacterium acidophilus' culture medium, 33 DEG C of culture 9h, obtain acidophilus
Lactobacillus suspension, described lactobacillus acidophilus culture medium in MRA culture mediums by adding inulin (also known as synanthrin), whey powder, junket egg
White hydrolysate and carrot juice obtain, and described MRA culture mediums include the parts by weight of peptone 15, the parts by weight of meat medicinal extract 15, yeast extract
7 parts by weight, the parts by weight of glucose 25, the parts by weight of sodium acetate 7, the parts by weight of Tween 80 1.5, Triammonium citrate 2.5
Parts by weight, the parts by weight of dipotassium hydrogen phosphate 2.5, the parts by weight of magnesium sulfate 0.3, the parts by weight of manganese sulfate 0.3, distilled water 1000
Parts by weight, its pH value are 6.2-6.4；The weight of described MRA culture mediums and inulin, whey powder, casein hydrolysate and carrot juice
Amount is than being 100：0.1：0.1：0.1：1.Described inulin, whey powder, casein hydrolysate and carrot juice is all commercially available prod.
By bacillus licheniformis by weight 2：100 are inoculated into LB culture mediums, 35 DEG C of culture 23h, obtain lichens gemma
Bacillus liquid.
(3) by the base-material of gained in the first step and saccharomyces cerevisiae bacterium solution, lactobacterium acidophilus' liquid, bacillus licheniformis liquid and water
By weight 100：4：4：7：45 mixing, adjust pH to 45.0, and in 40 DEG C of solid state fermentations 5 days, gained tunning used air-flow
Fluidized bed drying, leaving air temp are 45 DEG C, and EAT is 125 DEG C, is crushed, and crosses 1.0 eye mesh screens, and prevention Macrobrachium nipponensis property is made
The precocious biological agent of gland；Small peptide total amount >=28%, crude protein content >=70% are determined, molecular weight is less than 20000Dalton, ammonia
Base nitrogen is 0.8~1.0%, total nitrogen >=10%, lactic acid content >=3.0%, moisture≤10%.
Embodiment 4 prevents the preparation of the biological agent of Macrobrachium nipponensis Yearling, specifically comprises the following steps：
(1) by soybean meal 57wt%, rapeseed dregs 5wt%, fish meal 15wt%, vinasse dregs of rice 8wt%, cornstarch 5wt%,
Wheat bran 3wt%, molasses 3wt%, bloom 3wt% and sodium carbonate 1wt% mixing, obtain base-material, 110 DEG C of thermophilic digestion 0.5h disappear
Poison；
(2) by S. cervisiae by weight 5：100 are inoculated into S. cervisiae special culture media, 29 DEG C of culture 26h, obtain
To saccharomyces cerevisiae bacterium solution.
By lactobacterium acidophilus by weight 5：100 are inoculated into lactobacterium acidophilus' culture medium, 33 DEG C of culture 9h, obtain acidophilus
Lactobacillus suspension, described lactobacillus acidophilus culture medium in MRA culture mediums by adding inulin (also known as synanthrin), whey powder, junket egg
White hydrolysate and carrot juice obtain, and described MRA culture mediums include the parts by weight of peptone 15, the parts by weight of meat medicinal extract 15, yeast extract
7 parts by weight, the parts by weight of glucose 25, the parts by weight of sodium acetate 7, the parts by weight of Tween 80 1.5, Triammonium citrate 2.5
Parts by weight, the parts by weight of dipotassium hydrogen phosphate 2.5, the parts by weight of magnesium sulfate 0.3, the parts by weight of manganese sulfate 0.3, distilled water 1000
Parts by weight, its pH value are 6.2-6.4；The weight of described MRA culture mediums and inulin, whey powder, casein hydrolysate and carrot juice
Amount is than being 100：0.1：0.1：0.1：1.Described inulin, whey powder, casein hydrolysate and carrot juice is all commercially available prod.
By bacillus licheniformis by weight 5：100 are inoculated into LB culture mediums, 35 DEG C of culture 23h, obtain lichens gemma
Bacillus liquid.
(3) by the base-material of gained in the first step and saccharomyces cerevisiae bacterium solution, lactobacterium acidophilus' liquid, bacillus licheniformis liquid and water
By weight 100：4：4：7：45 mixing, adjust pH to 45.0, and in 40 DEG C of solid state fermentations 5 days, gained tunning used air-flow
Fluidized bed drying, leaving air temp are 45 DEG C, and EAT is 125 DEG C, is crushed, and crosses 1.0 eye mesh screens, and prevention Macrobrachium nipponensis property is made
The precocious biological agent of gland；Small peptide total amount >=30%, crude protein content >=65% are determined, molecular weight is less than 20000Dalton, ammonia
Base nitrogen is 0.8~1.0%, total nitrogen >=10%, lactic acid content >=3.0%, moisture≤10%.
The specific embodiment of the present invention is described in detail above, but it is intended only as example, it is of the invention and unlimited
It is formed on particular embodiments described above.To those skilled in the art, it is any to the equivalent modifications that carry out of the present invention and
Substitute also all among scope of the invention.Therefore, the impartial conversion made without departing from the spirit and scope of the invention and
Modification, all should be contained within the scope of the invention.
Claims (8)
1. a kind of biological agent for preventing Macrobrachium nipponensis Yearling, it is characterised in that by base-material and saccharomyces cerevisiae bacterium solution, acidophilus
Lactobacillus suspension, bacillus licheniformis liquid and water are by weight 100：3~4：3~4：5~7：35~45 mixing after solid state fermentation and
；Wherein, by weight percentage, the base-material by 50~70wt% of soybean meal, 4~5wt% of rapeseed dregs, fish meal 10~
15wt%, 5~8wt% of the vinasse dregs of rice, 2~5wt% of cornstarch, 1~3wt% of wheat bran, 1~3wt% of molasses, 1~3wt% of bloom
Formed with 0.5~1wt% of sodium carbonate.
2. biological agent according to claim 1, it is characterised in that the base-material and saccharomyces cerevisiae bacterium solution, lactobacterium acidophilus
The weight ratio of liquid, bacillus licheniformis liquid and water is 100：3.5~4：3~3.5：5.5~6.5：38~42.
3. biological agent according to claim 1, it is characterised in that percentage by weight meter, the base-material is by soybean meal 55
~65wt%, 4.2~4.6wt% of rapeseed dregs, 12~14wt% of fish meal, 6~8wt% of the vinasse dregs of rice, 3~4wt% of cornstarch, bran
1.5~2wt% of skin, 2~3wt% of molasses, 1.5~2wt% of bloom and 0.6~0.8wt% of sodium carbonate compositions.
4. a kind of preparation method for the biological agent for preventing Macrobrachium nipponensis Yearling as described in claim any one of 1-3, its
It is characterised by, comprises the following steps：
(1) by 50~70wt% of soybean meal, 4~5wt% of rapeseed dregs, 10~15wt% of fish meal, 5~8wt% of the vinasse dregs of rice, corn
2~5wt% of starch, 1~3wt% of wheat bran, 1~3wt% of molasses, 1~3wt% of bloom and 0.5~1wt% of sodium carbonate mixing, are obtained
To base-material, high-temperature sterilization；
(2) S. cervisiae, lactobacterium acidophilus and bacillus licheniformis are inoculated into respective culture medium respectively and cultivated,
Obtain saccharomyces cerevisiae bacterium solution, lactobacterium acidophilus' liquid and bacillus licheniformis liquid；
(3) base-material of gained in step (1) is pressed with saccharomyces cerevisiae bacterium solution, lactobacterium acidophilus' liquid, bacillus licheniformis liquid and water
Weight is than 100：3~4：3~4：5~7：35~45 mixing, adjust pH to 4.5~5.0, in 30~40 DEG C of solid state fermentations 3~5
My god, gained tunning is dry, crushes, and the biological agent of prevention Macrobrachium nipponensis Yearling is made.
5. the preparation method of biological agent according to claim 1, it is characterised in that S. cervisiae in the step (2)
The cultural method of liquid is：By S. cervisiae by weight 2~5：100 are inoculated into S. cervisiae special culture media, 29~
35 DEG C of 10~26h of culture, obtain saccharomyces cerevisiae bacterium solution.
6. the preparation method of biological agent according to claim 1, it is characterised in that lactobacterium acidophilus in the step (2)
The cultural method of liquid is：By lactobacterium acidophilus by weight 2~5：100 are inoculated into lactobacterium acidophilus' culture medium, 33~37 DEG C
9~25h is cultivated, obtains lactobacterium acidophilus' liquid.
7. the preparation method of biological agent according to claim 6, it is characterised in that lactobacterium acidophilus' culture medium by
MRA culture mediums are with inulin, whey powder, casein hydrolysate and carrot juice by weight 100：0.1~1：0.1~1：0.1~1：
1~10 is obtained by mixing.
8. the preparation method of biological agent according to claim 1, it is characterised in that lichens gemma bar in the step (2)
The cultural method of bacterium solution is：By bacillus licheniformis by weight 2~5：100 are inoculated into LB culture mediums, 35~38 DEG C of cultures
23~26h, obtain bacillus licheniformis liquid.
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Cited By (1)
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