CN105614107A - Feed additive capable of improving production performance of growing pigs and lowering diarrhea rate - Google Patents
Feed additive capable of improving production performance of growing pigs and lowering diarrhea rate Download PDFInfo
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Abstract
The invention discloses a preparation method of a feed additive capable of improving the production performance of growing pigs and lowering the diarrhea rate, which can replace antibiotics. The feed additive is prepared through taking ferrous glycine chelate, selenium yeast, saccharomyces cerevisiae cytoderm, vitamin additives, bacillus subtilis, saccharomyces cerevisiae, protease, cellulase, phytase, Neotame and an (I+G) flavoring agent as main components and taking fermented apple dreg and montmorillonite as carriers. The vitamin additives comprise vitamin A, vitamin D3 and vitamin E. The raw materials are compounded and all the raw materials have a synergistic effect. The feed additive has the functions of improving the organism immunity, adjusting the balance of intestinal flora and adsorbing mycotoxin, so that the diarrhea rate is lowered and the production performance of the growing pigs is improved.
Description
Technical field
The present invention relates to field of feed additive technology, be specially a kind of substitute antibiotics, improve grower pigs and produce performance and reduce the feed additive of diarrhea rate.
Background technology
Grower pigs refers to the pig in 25kg-60kg stage. The pig in this stage is growing way pig quickly, has a strong impact on the effect that the later stage fattens. A lot of producers mainly use antibiotic to keep healthy in this stage, cause the speed of growth relatively slow, produce drug resistance, make the matter worse especially especially for the pig treated before 25kg. Therefore a lot of plants occur in that a large amount of early stage growing way is better, the pig that later stage growing and fattening is slower. Expecting higher, raiser's profit is greatly reduced.
From in January, 2006 European Union completely forbid food animal use antibiotic feed additive for promoting growth, Denmark, Sweden etc. also in various degree prohibit the use of antibiotic, this allows for antibiotic use and is restricted. Thus substitute antibiotics, reduce drug resistance, recover intestinal health and set about just fundamentally improving the health of grower pigs, improve growth performance, then can solve the problems referred to above preferably.
Summary of the invention
The present invention is in order to overcome the problems referred to above, it is provided that a kind of substitute antibiotics, improves grower pigs and produces performance and reduce the feed additive of diarrhea rate.
The present invention adopts the following technical scheme that realization:
A kind of grower pigs that improves produces performance and reduces the feed additive of diarrhea rate, is made up of following raw material weight percentage ratio: brewing yeast cell wall 13-18%, yeast selenium 1-4%, ferrum glycinate 2-5%, vitamin A 0.01-0.03%, vitamin D30.01-0.04%, vitamin E 0.001-0.003%, antioxidant 0.1-0.3%, bacillus subtilis 0.5-3%, saccharomyces cerevisiae 0.4-0.7%, proteinase-10 .2-0.4%, cellulase 0.4-0.7%, phytase 0.8-1.2%, neotame 0.1-0.4%, (I+G) 0.5-2%, montmorillonite 5-10%, fermentation apple pomace 65-75%.
Feed additive optimum ratio (weight ratio): brewing yeast cell wall 15%, yeast selenium 1%, ferrum glycinate 2%, vitamin A 0.02%, vitamin D30.03%, vitamin E 0.002%, antioxidant 0.1%, bacillus subtilis 0.5%, saccharomyces cerevisiae 0.5%, proteinase-10 .2%, cellulase 0.4%, phytase 0.8%, neotame 0.3%, (I+G) 0.5%, montmorillonite 5%, fermentation apple pomace 75%.
Wherein, fermentation apple pomace: comprise the following steps: 1, the preparation of enterococcus faecalis bacterium solution, including the activation of strain, shake-flask seed cultivation, seed tank culture, fermentor cultivation. 2, concrete fermentation step: undertaken pulverizing (40 order) mixing by pomace, cottonseed meal, wheat bran, it is equipped with protease, cellulase, pectase, precipitated calcium carbonate, molasses and enterococcus faecalis bacterium solution, add water adjustment material moisture 40%, it is placed in check valve fermentation bag, in 30 DEG C of fermenting cellars, ferment 8 days, then dry to moisture less than 10%. After method provided by the present invention fermentation, in feedstuff, viable count reaches 1,000,000,000/g, crude protein >=25%, little peptide >=12%, free gossypol content��25mg/kg.
Using method: this feed additive directly makes an addition in grower pigs daily ration, addition is the 0.5-0.7% of growth swine rations gross weight.
Each raw material effect is as follows:
Brewing yeast cell wall: a kind of macromolecular polysaccharide complex extracted from yeast cell wall, main component is beta glucan (30%), mannooligo saccharide (30%), glycoprotein (20%) and other compositions, as chitin, protein, nucleic acid, lipoid and ash grade. There are raising growth of animal performance, carcass quality, immunity of organisms and mycotoxin adsorption effect etc.
Yeast selenium: the selenium in yeast mainly includes selenoenzyme, containing selenium egg matter and containing selenium polysaccharide class etc. Yeast selenium apparent digestibility improves about 10% than sodium selenite. Selenium can effectively protect cellularity integrity, can decompose lipid peroxide, removes lipid peroxidation free radical intermediate product, by aquation free radical scavenging or change into stable compound, reduces the outflow of cytosol, reduces drip loss. Can effectively alleviate the peroxide injury of cell, contribute to maintaining the normal oxidoreduction level of body, the immunologic function of enhancing body and the ability of anti-specific diseases. Selenium can affect the activity of superoxide dismutase, and selenium, as electronegative nonmetallic ion, has very strong affinity with metal, can be combined formation complex in vivo with the heavy metal such as hydrargyrum, chromium, lead, thus playing removing toxic substances and toxin expelling effect.
Ferrum glycinate: ferrum glycinate and ferrous sulfate (FeSO4) compare, chemical stability and good fluidity, there is higher biological value, and anemia and the immunologic function etc. of body can be improved. In pig source of iron biological value is tested, ferrous sulfate is generally 100% calculating, the piglet before 35 ages in days, and the biological value of aminoacid ferrum is the 122% of ferrous sulfate; And after 35 ages in days between 120 ages in days, the biological value of aminoacid ferrum is the 115% of ferrous sulfate. Improve the feed intake of animal, good palatability and animal body toxicity is little. Reduce the loss of vitamin in feedstuff. Amino-acid chelate is eaten after animal body, and transport plays a role to specific target site and enzyme system as a group in animal body, can stimulate the IgA level secreted simultaneously, is so favorably improved the resistance against diseases of body and improves the immunity of animal body.
Vitamin A, D, E: vitamin A, D, the supplementary promotion growth of animal of E, improves anti-stress ability, promotes Calcium and phosphorous absorption.
Bacillus subtilis, saccharomyces cerevisiae: consume intestinal oxygen, it is suppressed that harmful bacteria grows, create anaerobic environment for probiotics, promotes probiotics growth. Producing multiple digestive enzyme, metabolite promotes probiotics growth.
Protease, cellulase, phytase: reduce antinutritional factor in feedstuff, promote that protein and P elements utilize, improve efficiency of feed utilization.
Neotame, disodium 5 '-ribonucleotide (I+G): disodium 5 '-ribonucleotide (I+G) is the ucleotides food freshener of a new generation. Can be added directly in food, play the fresh effect of increasing. It it is relatively inexpensive and the best flavour enhancer of effect. Sweet taste and delicate flavour can cover taste uncomfortable in feedstuff, improve feedstuff palatability; Also can induction port solinocrine digestive enzyme, promote feedstuff digestion.
Montmorillonite: absorbing mycotoxin, reduces toxin to intestinal injury.
Fermentation apple pomace: rich in dietary fiber, vitamin, the lactic acid of trace element and antibacterial metabolism, enzyme, somatomedin etc. Efficiency of feed utilization can be improved, provide nutrient substance, regulating intestinal canal colony balance for intestinal beneficial bacterium.
Feed additive of the present invention is with rich in vitamin, tropina, the dry fermentation apple pomace of somatomedin isoreactivity material and the montmorillonite with absorbing mycotoxin are diluent, composite brewing yeast cell wall, yeast selenium, ferrum glycinate, vitamin A, vitamin D3, vitamin E, bacillus subtilis, saccharomyces cerevisiae, protease, cellulase, phytase, this feed additive can improve grower pigs immunity of organisms, regulates and suppresses harmful intestinal tract bacteria growing, promotes the breeding of probiotics; Mycotoxin in adsorption feed; Repair damaged intestine. Thus reducing diarrhoea, digesting and assimilating of the composition that has additional nutrients, increase immunity of organisms, and the antibiotic in alternative daily ration, thus improving grower pigs growth performance and health level.
Detailed description of the invention
Below specific embodiments of the invention are described in detail.
Embodiment 1
A kind of grower pigs that improves produces performance and reduces the feed additive of diarrhea rate, and proportioning (weight ratio) is as follows:
Brewing yeast cell wall 15%, yeast selenium 1%, ferrum glycinate 2%, vitamin A 0.02%, vitamin D30.03%, vitamin E 0.002%, antioxidant 0.1%, bacillus subtilis 0.5%, saccharomyces cerevisiae 0.5%, proteinase-10 .2%, cellulase 0.4%, phytase 0.8%, neotame 0.3%, (I+G) 0.5%, montmorillonite 5%, fermentation apple pomace 75%.
Feeding effect:
Test prepares: 200 dates of birth of test and Selection are close, and the DLY tri-crossbreeding of about body weight 30kg carries out feeding experiment in the 7-9 month in 2015 on Ruicheng County pig farm. Feeding experiment carries out random packet in units of circle. Random point 5 groups: respectively matched group, test 1,2,3,4 groups, often group 5 circle, often 8, circle, control group fed basal diet (antibiotic-free), test 1,2,3 groups are added 0.5%, 0.6%, 0.7% additive respectively in basal diet, test 4 groups and use colistin and bacitracin zinc complex antimicrobials in basal diet. It is essentially identical that each group piglet number and piglet start age in days, without significant difference. 40 days experimental periods.
Process of the test: effectively feeding time is 40 days, grower pigs free choice feeding during test, freely drink water, claims litter weight when on-test and end, record feed intake every day in experimentation, and observes the diarrhoea head number often enclosed every day.
Result of the test: it is shown that add 0.5%, 0.6%, 0.7% additive in daily ration not only can improve grower pigs entirety growth performance, and diarrhea rate can be greatly reduced. From subordinate list, test 1 compared with matched group, 2,3,4 groups, daily ration adds 0.5%, 0.6%, 0.7% additive and adds complex antimicrobials, the production performance that can make grower pigs increases substantially, wherein grower pigs weightening finish raising 8.58%, 8.61%, 8.79%, 8.70%, and survival rate improves 5.26%, 2.63%, 5.26%, 5.26%, feed-weight ratio reduces by 6.63%, 6.61%, 6.74%, 6.77%, and grower pigs diarrhea rate significantly reduces 49.5%, 50.6%, 51.6%, 50.2%.
Conclusion (of pressure testing): add this additive of 0.5-0.7% in daily ration and can significantly improve weightening finish and reduce feed-weight ratio, significantly reduces diarrhea rate simultaneously, and effect is not notable with interpolation antibiotic group difference.
Test detailed results is in Table 1:
Table 1
Embodiment 2
A kind of grower pigs that improves produces performance and reduces the feed additive of diarrhea rate, and proportioning (weight ratio) is as follows:
Brewing yeast cell wall 13%, yeast selenium 2%, ferrum glycinate 4%, vitamin A 0.03%, vitamin D30.04%, vitamin E 0.003%, antioxidant 0.3%, bacillus subtilis 0.5%, saccharomyces cerevisiae 0.6%, proteinase-10 .4%, cellulase 0.5%, phytase 0.8%, neotame 0.2%, (I+G) 1.5%, montmorillonite 10%, fermentation apple pomace 70%.
Using method: the addition of this feed additive is the 0.6% of growth swine rations gross weight.
Embodiment 3
A kind of grower pigs that improves produces performance and reduces the feed additive of diarrhea rate, and proportioning (weight ratio) is as follows:
Brewing yeast cell wall 13%, yeast selenium 4%, ferrum glycinate 4%, vitamin A 0.02%, vitamin D30.01%, vitamin E 0.002%, antioxidant 0.1%, bacillus subtilis 2%, saccharomyces cerevisiae 0.4%, proteinase-10 .4%, cellulase 0.7%, phytase 1%, neotame 0.1%, (I+G) 1%, montmorillonite 5%, fermentation apple pomace 70%.
Using method: the addition of this feed additive is the 0.7% of growth swine rations gross weight.
Embodiment 4
A kind of grower pigs that improves produces performance and reduces the feed additive of diarrhea rate, and proportioning (weight ratio) is as follows:
Brewing yeast cell wall 18%, yeast selenium 3%, ferrum glycinate 2%, vitamin A 0.01%, vitamin D30.02%, vitamin E 0.001%, antioxidant 0.2%, bacillus subtilis 2%, saccharomyces cerevisiae 0.7%, proteinase-10 .3%, cellulase 0.6%, phytase 1.2%, neotame 0.4%, (I+G) 2%, montmorillonite 5%, fermentation apple pomace 65%.
Using method: the addition of this feed additive is the 0.7% of growth swine rations gross weight.
In above-described embodiment, the preparation method of fermentation apple pomace is as follows:
, preparation in enterococcus faecalis bacterium solution
(1), the activation of strain: for improving the vigor of strain, before connecing shaking flask, it is necessary to strain is activated. After the enterococcus faecalis normal saline dilution that inclined-plane is preserved, coat on MRS solid plate, cultivate 24h for 33 DEG C, single bacterium colony that picking transparent circle is bigger, carry out plate isolation line, cultivate 24h for 30 DEG C, the bigger single bacterium colony of picking transparent circle is on MRS solid plate, cultivate 24h for 33 DEG C, single bacterium colony that on last picking MRS flat board, transparent circle is bigger, accesses deep layer MRS liquid tube, after 30-35 DEG C of cultivation 12-16h, access MRS fluid medium with 1% inoculum concentration, cultivate 18-24h as seed liquor. Wherein, described strain activation and culture based formulas is, peptone 5-15g/L, Carnis Bovis seu Bubali cream 5-15g/L, yeast leaching powder 3-10g/L, glucose 10-25g/L, dibasic ammonium citrate 1-4g/L, sodium acetate 2-7g/L, dipotassium hydrogen phosphate 1-5g/L, Magnesium sulfate heptahydrate 0.25-0.65g/L, manganese sulfate monohydrate 0.1-0.3g/L, pH6.0-6.5.
(2), shake-flask seed is cultivated:
One-level shake-flask seed is cultivated: according to the inoculum concentration of 5-10%, the slant strains after activation being received one-level Shake flask medium, 250ml triangular flask liquid amount 100ml, 30-35 DEG C quiescent culture 12-16h, as one-level shake-flask seed liquid. Wherein, described one-level shake-flask culture based formulas is: peptone 5-15g/L, Carnis Bovis seu Bubali cream 5-15g/L, yeast leaching powder 3-10g/L, glucose 10-25g/L, dibasic ammonium citrate 1-4g/L, sodium acetate 2-7g/L, dipotassium hydrogen phosphate 1-5g/L, Magnesium sulfate heptahydrate 0.25-0.65g/L, manganese sulfate monohydrate 0.1-0.3g/L, pH6.0-6.5.
Second-level shake flask seed culture: according to the inoculum concentration of 5-10%, is linked in second-level shake flask culture medium by one-level shake-flask seed liquid, and 500ml triangular flask liquid amount 250ml, quiescent culture 16-20h in 30-35 DEG C of incubator, as second-level shake flask seed liquor. Wherein, described second-level shake flask culture medium prescription is: soybean cake powder 15-25g/L, peptone 5-15g/L, yeast leaching powder 5-15g/L, glucose 15-25g/L, calcium carbonate 0.5-1.5g/L, dibasic ammonium citrate 1-4g/L, sodium acetate 2-7g/L, dipotassium hydrogen phosphate 1-5g/L, Magnesium sulfate heptahydrate 0.25-0.65g/L, manganese sulfate monohydrate 0.1-0.3g/L, pH6.0-6.5.
(3), seed tank culture:
First class seed pot is cultivated: receive in first class seed pot culture medium according to 2-5% inoculum concentration by second-level shake flask seed liquor, the canned liquid coefficient of first order seed is 50%, cultivation temperature 30-35 DEG C, tank pressure 0.03-0.05MPa, stir cultivation 12-18h under 100-200 rev/min of condition, treat that total bacteria count reaches 1-3 �� 109During more than CFU/ml, proceed in secondary seed tank culture medium and cultivate. Wherein, the formula of described first class seed pot culture medium is: soybean cake powder 25-35g/L, peptone 5-15g/L, yeast leaching powder 5-15g/L, glucose 15-25g/L, calcium carbonate 0.5-1.5g/L, dibasic ammonium citrate 1-4g/L, sodium acetate 2-7g/L, dipotassium hydrogen phosphate 1-5g/L, Magnesium sulfate heptahydrate 0.25-0.65g/L, manganese sulfate monohydrate 0.1-0.3g/L, pH6.0-6.5.
Secondary seed tank is cultivated: receive in secondary seed tank culture medium according to 5-8% inoculum concentration by first class seed pot culture fluid, the canned liquid coefficient of secondary seed is 55%, cultivation temperature 30-35 DEG C, tank pressure 0.03-0.05MPa, stir cultivation 14-20h under 100-200 rev/min of condition, treat that total bacteria count reaches 4-7 �� 109During more than CFU/ml, proceed to fermentation tank and cultivate. Wherein, the formula of described secondary seed tank culture medium is: soybean cake powder 25-35g/L, peptone 5-15g/L, yeast leaching powder 5-15g/L, glucose 15-25g/L, calcium carbonate 0.5-1.5g/L, dibasic ammonium citrate 1-4g/L, sodium acetate 2-7g/L, dipotassium hydrogen phosphate 1-5g/L, Magnesium sulfate heptahydrate 0.25-0.65g/L, manganese sulfate monohydrate 0.1-0.3g/L, pH6.0-6.5.
(4), fermentor cultivation:
Fermentation tank High Density Cultivation: according to the inoculum concentration of 5-10%, secondary seed tank seed liquor is proceeded to the fermentation basal medium of fermentation tank, at temperature 30-35 DEG C, stir 100-120 rev/min, ferment when tank pressure 0.03-0.05MPa, cultivate with this understanding about 3 hours, when fermentation liquid residual sugar drops to below 0.8%, mend sugar once per hour, after mending sugar, culture fluid residual sugar controls below 2%. Along with the carrying out of fermentation, enterococcus faecalis produces acid, and fermentation liquid pH declines, when fermentation liquid pH is lower than 6.0, and the NaOH of auto-feeding 2mol/L, supplement alkali liquor and regulate pH to 6.5-7.0, incubation time is 22-24 hour. Wherein, described fermentation basal medium formulation is: soybean cake powder 25-35g/L, peptone 5-15g/L, yeast leaching powder 5-15g/L, glucose 15-25g/L, calcium carbonate 0.5-1.5g/L, dibasic ammonium citrate 1-4g/L, sodium acetate 2-7g/L, dipotassium hydrogen phosphate 1-5g/L, Magnesium sulfate heptahydrate 0.25-0.65g/L, manganese sulfate monohydrate 0.1-0.3g/L, pH6.0-6.5. Described fermentation medium supplementing culture medium formula is glucose solution, and in described glucose solution, glucose content is the 2-4% of fermentation liquid total amount.
, concrete fermentation step:
Constituting mixture after the ratio of pomace 50-80 part, cottonseed meal 20-40 part, wheat bran 20-30 part, precipitated calcium carbonate 3-5 part being mixed, wherein, total number of pomace, cottonseed meal, wheat bran and precipitated calcium carbonate is 100; Again with mixture weight for counting, add cellulase 0.05-0.07%, proteinase-10 .03-0.05%, pectase 0.02-0.07%, molasses 0.02-0.3%, enterococcus faecalis bacterium solution 8-20%, add water adjustment material moisture at 30-45%, it is placed in check valve fermentation bag, in 15-30 DEG C of fermenting cellar, ferments 5-15 days, in feedstuff, viable count reaches 2,000,000,000/more than g, crude protein >=15%, little peptide >=10%, free gossypol content��25mg/kg.
Wherein, protease, enzyme activity >=105U/g, addition is 300-500g/t; Cellulase, enzyme activity >=105U/g, addition 500-700g/t; Pectase, enzyme activity >=30000U/g, addition 200-700g/t.
It should be noted last that; above example is only in order to illustrate technical scheme and unrestricted; although the present invention being described in detail with reference to embodiment; it will be understood by those within the art that; technical scheme is modified or equivalent replacement; without departure from the spirit and scope of technical scheme, it all should be contained in the claims of the present invention.
Claims (10)
1. one kind is improved grower pigs production performance and reduces the feed additive of diarrhea rate, it is characterised in that: it is made up of following raw material weight percentage ratio:
Brewing yeast cell wall 13-18%, yeast selenium 1-4%, ferrum glycinate 2-5%, vitamin A 0.01-0.03%, vitamin D30.01-0.04%, vitamin E 0.001-0.003%, antioxidant 0.1-0.3%, bacillus subtilis 0.5-3%, saccharomyces cerevisiae 0.4-0.7%, proteinase-10 .2-0.4%, cellulase 0.4-0.7%, phytase 0.8-1.2%, neotame 0.1-0.4%, (I+G) 0.5-2%, montmorillonite 5-10%, fermentation apple pomace 65-75%.
2. raising grower pigs according to claim 1 produces performance and reduces the feed additive of diarrhea rate, it is characterised in that: it is made up of the raw material of following weight percentage ratio:
Brewing yeast cell wall 15%, yeast selenium 1%, ferrum glycinate 2%, vitamin A 0.02%, vitamin D30.03%, vitamin E 0.002%, antioxidant 0.1%, bacillus subtilis 1%, saccharomyces cerevisiae 0.5%, proteinase-10 .2%, cellulase 0.4%, phytase 0.8%, neotame 0.3%, (I+G) 0.5%, montmorillonite 5%, fermentation apple pomace 75%.
3. raising grower pigs according to claim 1 and 2 produces performance and reduces the feed additive of diarrhea rate, it is characterised in that: the preparation method of described fermentation apple pomace is as follows:
, enterococcus faecalis bacterium solution preparation
(1), the activation of strain: the enterococcus faecalis that inclined-plane preserves is activated 3 times on activation medium, single bacterium colony that on picking MRS flat board, transparent circle is bigger, access deep layer MRS liquid tube, after 30-35 DEG C of cultivation 12-16h, access MRS fluid medium with 1% inoculum concentration, cultivate 18-24h as seed liquor;
(2), shake-flask seed is cultivated:
One-level shake-flask seed is cultivated: being accessed in one-level Shake flask medium according to the inoculum concentration of 5-10% by the enterococcus faecalis activated, 30-35 DEG C of quiescent culture 12-16h, as one-level shake-flask seed liquid;
Second-level shake flask seed culture: one-level shake-flask seed liquid is linked in second-level shake flask culture medium according to the inoculum concentration of 5-10%, quiescent culture 16-20h in 30-35 DEG C of incubator, as second-level shake flask seed liquor;
(3), seed tank culture:
First class seed pot is cultivated: receive in first class seed pot culture medium according to 2-5% inoculum concentration by second-level shake flask seed liquor, cultivation temperature 30-35 DEG C, tank pressure 0.03-0.05MPa, stirs cultivation 12-18h under 100-200 rev/min of condition, treats that total bacteria count reaches 1-3 �� 109During more than CFU/ml, proceed in secondary seed tank culture medium and cultivate;
Secondary seed tank is cultivated: receive in secondary seed tank culture medium according to 5-8% inoculum concentration by first class seed pot seed liquor, cultivation temperature 30-35 DEG C, tank pressure 0.03-0.05MPa, stirs cultivation 14-20h under 100-200 rev/min of condition, treats that total bacteria count reaches 4-7 �� 109During more than CFU/ml, proceed to fermentation tank and cultivate;
(4), fermentor cultivation:
According to the inoculum concentration of 5-10%, secondary seed tank seed liquor is proceeded to the fermentation basal medium of fermentation tank, at temperature 30-35 DEG C, stir 100-120 rev/min, ferment when tank pressure 0.03-0.05MPa, fermentation to fermentation basal medium residual sugar content below 0.8% time, with fermentation medium supplementing culture medium mend sugar, after mending sugar, in culture fluid, residual sugar controls at 1-2%, in sweat, regulating pH at 6.0-6.5, incubation time is 22-24 hour;
, concrete fermentation step:
By pomace, cottonseed meal, wheat bran, precipitated calcium carbonate mixing, it is equipped with protease, cellulase, pectase, molasses and enterococcus faecalis bacterium solution, add water adjustment material moisture at 30-45%, it is placed in round, in 15-30 DEG C of fermenting cellar, ferments 5-15 days, in feedstuff, viable count reaches 2,000,000,000/more than g, crude protein >=15%, little peptide >=10%, free gossypol content��25mg/kg.
4. raising grower pigs according to claim 3 produces performance and reduces the feed additive of diarrhea rate, it is characterised in that:
StepIn, constitute mixture after the ratio of pomace 50-80 part, cottonseed meal 20-40 part, wheat bran 20-30 part, precipitated calcium carbonate 3-5 part being mixed, wherein, total number of pomace, cottonseed meal, wheat bran and precipitated calcium carbonate is 100; Again with mixture weight for counting, add cellulase 0.05-0.07%, proteinase-10 .03-0.05%, pectase 0.02-0.07%, molasses 0.02-0.3%, enterococcus faecalis bacterium solution 8-20%.
5. raising grower pigs according to claim 4 produces performance and reduces the feed additive of diarrhea rate, it is characterised in that: protease, enzyme activity >=105U/g, addition is 300-500g/t; Cellulase, enzyme activity >=105U/g, addition 500-700g/t; Pectase, enzyme activity >=30000U/g, addition 200-700g/t.
6. raising grower pigs according to claim 3 produces performance and reduces the feed additive of diarrhea rate, it is characterised in that: in step (1), described strain activation and culture based formulas is, peptone 5-15g/L, Carnis Bovis seu Bubali cream 5-15g/L, yeast leaching powder 3-10g/L, glucose 10-25g/L, dibasic ammonium citrate 1-4g/L, sodium acetate 2-7g/L, dipotassium hydrogen phosphate 1-5g/L, Magnesium sulfate heptahydrate 0.25-0.65g/L, manganese sulfate monohydrate 0.1-0.3g/L, pH6.0-6.5.
7. raising grower pigs according to claim 3 produces performance and reduces the feed additive of diarrhea rate, it is characterized in that: in step (2), described one-level shake-flask culture based formulas is: peptone 5-15g/L, Carnis Bovis seu Bubali cream 5-15g/L, yeast leaching powder 3-10g/L, glucose 10-25g/L, dibasic ammonium citrate 1-4g/L, sodium acetate 2-7g/L, dipotassium hydrogen phosphate 1-5g/L, Magnesium sulfate heptahydrate 0.25-0.65g/L, manganese sulfate monohydrate 0.1-0.3g/L, pH6.0-6.5;
Described second-level shake flask culture medium prescription is: soybean cake powder 15-25g/L, peptone 5-15g/L, yeast leaching powder 5-15g/L, glucose 15-25g/L, calcium carbonate 0.5-1.5g/L, dibasic ammonium citrate 1-4g/L, sodium acetate 2-7g/L, dipotassium hydrogen phosphate 1-5g/L, Magnesium sulfate heptahydrate 0.25-0.65g/L, manganese sulfate monohydrate 0.1-0.3g/L, pH6.0-6.5.
8. raising grower pigs according to claim 3 produces performance and reduces the feed additive of diarrhea rate, it is characterised in that: in step (3), the formula of described first class seed pot culture medium is: soybean cake powder 25-35g/L, peptone 5-15g/L, yeast leaching powder 5-15g/L, glucose 15-25g/L, calcium carbonate 0.5-1.5g/L, dibasic ammonium citrate 1-4g/L, sodium acetate 2-7g/L, dipotassium hydrogen phosphate 1-5g/L, Magnesium sulfate heptahydrate 0.25-0.65g/L, manganese sulfate monohydrate 0.1-0.3g/L, pH6.0-6.5;
The formula of described secondary seed tank culture medium is: soybean cake powder 25-35g/L, peptone 5-15g/L, yeast leaching powder 5-15g/L, glucose 15-25g/L, calcium carbonate 0.5-1.5g/L, dibasic ammonium citrate 1-4g/L, sodium acetate 2-7g/L, dipotassium hydrogen phosphate 1-5g/L, Magnesium sulfate heptahydrate 0.25-0.65g/L, manganese sulfate monohydrate 0.1-0.3g/L, pH6.0-6.5.
9. raising grower pigs according to claim 3 produces performance and reduces the feed additive of diarrhea rate, it is characterised in that: in step (4), described fermentation basal medium formulation is: soybean cake powder 25-35g/L, peptone 5-15g/L, yeast leaching powder 5-15g/L, glucose 15-25g/L, calcium carbonate 0.5-1.5g/L, dibasic ammonium citrate 1-4g/L, sodium acetate 2-7g/L, dipotassium hydrogen phosphate 1-5g/L, Magnesium sulfate heptahydrate 0.25-0.65g/L, manganese sulfate monohydrate 0.1-0.3g/L, pH6.0-6.5;
Described fermentation medium supplementing culture medium formula is glucose solution, and in described glucose solution, glucose content is the 2-4% of fermentation liquid total amount.
10. raising grower pigs according to claim 3 produces performance and reduces the feed additive of diarrhea rate, it is characterised in that: stepIn, round selects check valve fermentation bag.
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| CN (1) | CN105614107A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106260671A (en) * | 2016-10-31 | 2017-01-04 | 河南普尼尔生物制药有限公司 | A kind of intestinal health-care agent improving parturition lactation capacity of milk secretion of sow and preparation method thereof and using method |
| CN106359943A (en) * | 2016-10-18 | 2017-02-01 | 杨继刚 | Feed additive capable of improving producing performance and foraging performance of early-weaned lamb |
| CN106509440A (en) * | 2016-11-16 | 2017-03-22 | 山西大禹生物工程股份有限公司 | Health-care feed additive for multiparous sows |
| CN106509439A (en) * | 2016-11-16 | 2017-03-22 | 山西大禹生物工程股份有限公司 | Feed additive replacingantibiotics for preventing weaned piggies from suffering from diarrhoea |
| CN106562077A (en) * | 2016-10-12 | 2017-04-19 | 浙江海洋大学 | Feed additive for reducing diarrhea rate of piglets as well as preparation method and application thereof |
| CN106689671A (en) * | 2016-12-28 | 2017-05-24 | 新昌县赛因斯生物科技有限公司 | Biological bacterium-containing livestock feed and preparation method thereof |
| CN107494927A (en) * | 2017-08-02 | 2017-12-22 | 陆丽英 | The method that sugarcane recycles |
| CN109601723A (en) * | 2018-12-06 | 2019-04-12 | 唐山拓普生物科技有限公司 | A kind of fur-bearing animal anti-diarrhea, growth-promoting feed and preparation process and feeding method |
| US11197902B2 (en) * | 2016-06-24 | 2021-12-14 | Ig Biosciences Corporation | Prebiotic neutraceutical compositions and methods of treatment using the same |
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| CN101352198A (en) * | 2008-08-15 | 2009-01-28 | 天津生机集团股份有限公司 | Method for preparing Enterococcus faecalis fermented mixed feed for birds |
| CN103988977A (en) * | 2014-05-28 | 2014-08-20 | 河南牧业经济学院 | Feed microecological preparation and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101352198A (en) * | 2008-08-15 | 2009-01-28 | 天津生机集团股份有限公司 | Method for preparing Enterococcus faecalis fermented mixed feed for birds |
| CN103988977A (en) * | 2014-05-28 | 2014-08-20 | 河南牧业经济学院 | Feed microecological preparation and preparation method thereof |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11197902B2 (en) * | 2016-06-24 | 2021-12-14 | Ig Biosciences Corporation | Prebiotic neutraceutical compositions and methods of treatment using the same |
| CN106562077A (en) * | 2016-10-12 | 2017-04-19 | 浙江海洋大学 | Feed additive for reducing diarrhea rate of piglets as well as preparation method and application thereof |
| CN106359943A (en) * | 2016-10-18 | 2017-02-01 | 杨继刚 | Feed additive capable of improving producing performance and foraging performance of early-weaned lamb |
| CN106260671A (en) * | 2016-10-31 | 2017-01-04 | 河南普尼尔生物制药有限公司 | A kind of intestinal health-care agent improving parturition lactation capacity of milk secretion of sow and preparation method thereof and using method |
| CN106509440A (en) * | 2016-11-16 | 2017-03-22 | 山西大禹生物工程股份有限公司 | Health-care feed additive for multiparous sows |
| CN106509439A (en) * | 2016-11-16 | 2017-03-22 | 山西大禹生物工程股份有限公司 | Feed additive replacingantibiotics for preventing weaned piggies from suffering from diarrhoea |
| CN106509439B (en) * | 2016-11-16 | 2019-11-05 | 山西大禹生物工程股份有限公司 | The feed addictive of substitute antibiotics preventing post-weaning diarrhea |
| CN106689671A (en) * | 2016-12-28 | 2017-05-24 | 新昌县赛因斯生物科技有限公司 | Biological bacterium-containing livestock feed and preparation method thereof |
| CN107494927A (en) * | 2017-08-02 | 2017-12-22 | 陆丽英 | The method that sugarcane recycles |
| CN109601723A (en) * | 2018-12-06 | 2019-04-12 | 唐山拓普生物科技有限公司 | A kind of fur-bearing animal anti-diarrhea, growth-promoting feed and preparation process and feeding method |
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Application publication date: 20160601 |