CN101642187B - Protein feed rich in small peptides and multi-enzyme bacteria and preparation method thereof - Google Patents
Protein feed rich in small peptides and multi-enzyme bacteria and preparation method thereof Download PDFInfo
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- CN101642187B CN101642187B CN2009100660180A CN200910066018A CN101642187B CN 101642187 B CN101642187 B CN 101642187B CN 2009100660180 A CN2009100660180 A CN 2009100660180A CN 200910066018 A CN200910066018 A CN 200910066018A CN 101642187 B CN101642187 B CN 101642187B
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Abstract
The invention relates to protein feed rich in small peptides and multi-enzyme strains and a preparation method thereof. The protein feed is prepared by fermenting soybean meal, cottonseed meal, rapeseed meal, bone meal, wheat bran, common salt, light calcium carbonate, water and strains in a fermentation bin. The preparation method of the protein feed comprises the following steps: mixing the soybean meal, the cottonseed meal, the rapeseed meal, the bone meal, the wheat bran, the common salt and the light calcium carbonate in percentage by weight; adding 20-30 percent of water; sending the mixture to an air steamer; adding high-pressure steam to the air steamer so as to increase the air pressure to 1-2 Mpa and the temperature to 140-200 DEG C; keeping the pressure for 4-6 minutes; momentarily releasing the mixture in the air steamer; sending the mixture to the fermentation bin; inoculating 3-5 percent of strains when the temperature of the mixture falls to 30-37 DEG C; fermenting for 72 hours at the temperature of 32-37 DEG C; discharging from the bin; and bagging to obtain a finished product. The product has strong disease-resistant function and low cost, increases the production of animal by-products by more than 20 percent, is nontoxic, harmless and pollution-free and can inhibit food-borne diseases represented in cancers and subhealth phenomena from spreading.
Description
Technical field
The present invention relates to a kind of protein feed, specifically a kind of protein feed and preparation method who is rich in little peptide and multi-enzyme bacteria.
Background technology
At present owing to high-quality protein feed, like fish meal, dregs of beans etc., selling at exorbitant prices; Seriously influence aquaculture cost; Adopt new and high technology, do not increasing under the condition of cost, improve the utilization rate of protein feed; Improving the function (immunologic function, growth promotion function) of protein feed, is China's problem demanding prompt solution.
Protein is a kind of nitrogenous macromolecular organic compound; Be the basis of formation animal and plant body cell and tissue, organ; It mainly is made up of carbon (C), hydrogen (H), oxygen (O), nitrogen (N) and a spot of sulphur (s) element; These elements at first are combined into amino acid molecular, aggregate into peptide chain and multiple steric configuration again to embody its major physiological function.Its effect is as follows: (1) protein is the various tissues of structural material animal body, organ and various product such as muscle, skin, internal organ, blood, nerve, bone, breast, the egg of animal body etc., all is to be formed as structural material by protein.Protein is to dewater in the animal body exceptionally, and the material that content is the highest can account for about 50% of animal body solid content usually.Protein contents such as some histoorgan such as muscle, liver and spleen can be up to more than 80%.Why various histoorgans have specific physiological function, mainly be because of the kinds of protein of forming this histoorgan with due to existence form is different.For example, globulin is the key component of constituting body tissue, and albumin is the main component that constitutes body fluid, and scleroprotein then is the key component that constitutes bone, muscle tendon, ligament, hair etc.Therefore, physiological and biochemical procedures such as the gestation of animal body, growth, lactation, product hair, all with particular proteins as material base.(2) protein be upgrade soma essential material animal body in metabolic processes, the continuous decomposition of histocyte through protein upgraded with synthesizing.This self process, the essential characteristic of life just.Be exactly adults, grow stop basically after, also need constantly take the photograph human protein, with the synthetic need of added body histone.(3) protein is many important function materials in the interior functional mass animal body of animal body, like catalysis and enzyme and the hormone of regulating metabolic process, strengthens the immune body of animal defense function and raising premunition etc., all is that main body constitutes with protein.In addition, the keeping of acid-base balance, the transmission of hereditary information and the transhipment of many important substance etc. are relevant with protein invariably in the animal body.(4) protein can be made for the energy substance animal body take the photograph human protein too much or different quality close when requiring, also can be oxidized and give off energy.And as the energy substance of animal body, though the major physiological function of protein and non-oxide energy supply.1 gram protein during oxidation, can produce the heat of 17.15 kilojoules in vivo.During the protein undersupply, the animal body protein metabolism becomes negative balance in the feed, and the weight of animals alleviates, and grows slowly, and premunition descends.Public beastly sperm quantity minimizing can appear in the animal of breeding period, semen quality reduces; Female beast oestrus and sexual cycle unusual, be difficult for becoming pregnant or development of fetus bad, even produce monster, stillborn foetus etc.Protein is too much in the feed, not only causes waste, and can cause animal body metabolic disorder and protein poisoning.
In recent years, the animal husbandry of China has had significant progress, and the requirement of animal protein feed is increased day by day.Along with the progressively forbidding of worldwide livestock and poultry offal, blood meal etc., the exploitation vegetable protein is the new way that solves animal protein feed problem in short supply.The approach that the present invention deals with problems is: the multiple proteins forage crop, rationally utilize.After special processing, contain abundant thick protein like beans cake, wheat bran etc., and good palatability, be good protein feeds.
The present invention utilizes the quick-fried technology of vapour can crack protein, produces the oligopeptides material, can make the assorted dregs of rice detoxification of part of interpolation again, improves its utilization rate.In multi-strain fermentation, through aerobic and anaerobic fermentation, microorganism is bred in a large number, its result has not only improved the protein level of fermentation raw material, and Partial Protein is converted into mycoprotein when fermentation.In fermentation, the degradation product of protein comprises polypeptide, oligopeptides, amino acid etc., has globality, comprehensive and concertedness simultaneously.The active absorption function of particularly little peptide; Have than amino acid sooner, higher speed and efficient, and do not receive amino acid whose the influence, product of the present invention both can replace the protein feed of high-quality; Feed addictives such as little peptide, multienzyme and probio have been avoided repeating to add again; Not only reduced aquaculture cost, can increase the yield and quality of meat, eggs and milk again, can make the quality of meat, eggs and milk reach green food standard.
Summary of the invention
The object of the invention is intended to solve the deficiency of protein feed production technology, and a kind of protein feed and preparation method who is rich in little peptide and multi-enzyme bacteria is provided, to improve the quality and the function of protein raw materials; Produce high-quality protein feed, and utilize the vapour explosion to separate protein raw materials, handle through microbial fermentation again; Make it produce a large amount of little peptide, amino acid; Contain a large amount of B family vitamin, plurality of enzymes simultaneously, not only the instead high-quality protein feed also has the disease-resistant function of growth promotion simultaneously; To fill up the blank of China in this production technology, satisfy the demand of domestic and international market.
The technical scheme that the present invention adopts is: a kind of protein feed that is rich in little peptide and multi-enzyme bacteria, adopt dregs of beans, cotton dregs, the dish dregs of rice, bone meal, wheat bran, salt, precipitated calcium carbonate, water and bacterial classification, and after the fermentation cabin fermentation, process; The percentage by weight that dregs of beans, cotton dregs, the dish dregs of rice, bone meal, wheat bran, salt, precipitated calcium carbonate and water add is: dregs of beans 50-60%, cotton dregs 10-15%, dish dregs of rice 10-15%; Bone meal 5-8%; Wheat bran 10-15%, salt 0.15%, precipitated calcium carbonate 0.7%; Water 20-30%, the percentage by weight that bacterial classification adds is: the 3-5% of the gross weight of dregs of beans, cotton dregs, the dish dregs of rice, bone meal, wheat bran, salt, precipitated calcium carbonate and water.
The bacterial classification that the present invention adopted; Be made up of bacillus subtilis, bafillus natto, bacillus licheniformis, brewer's yeast, product Ruan Candida, Lactobacillus plantarum and bifidobacterium bifidum, the percentage by weight that each bacterial classification adds is: bacillus subtilis 10-15%, bafillus natto 10-15%, bacillus licheniformis 10-15%, brewer's yeast 10-15%, product Ruan Candida 15-20%, Lactobacillus plantarum 10-15% and bifidobacterium bifidum 10-15%.
Above-mentioned bacterial classification is cultivated through test tube cultivation, shaking table cultivation, first class seed pot respectively, carried out deep-layer liquid by 3-5% access secondary seed jar and cultivate, cultivation temperature 28-37 ℃, incubation time is 24-72h; Viable count is greater than 10
9Individual/milliliter.
The present invention, the seed culture medium of said seeding tank is: brown sugar 5-8%, dregs of beans 1-2%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.15%, precipitated calcium carbonate 1%, surplus is a water, and initial p H value is transferred to 7.0.
The bacterial classification that the present invention adopts all is known bacterial classification, can introduce through Chinese common micro-organisms culture presevation administrative center or the Chinese Academy of Agricultural Sciences microorganism fungus kind preservation center, is applied in the industrialization through screening.
The present invention, a kind of preparation method who is rich in the protein feed of little peptide and multi-enzyme bacteria, carry out according to following method step:
(1), by weight percentage get dregs of beans 50-60%, cotton dregs 10-15%, dish dregs of rice 10-15%, bone meal 5-8%, wheat bran 10-15%, salt 0.15% and precipitated calcium carbonate 0.7%, mix, process compound, after add the water of 20-30% by weight percentage; Make the moisture of compound reach 25%, subsequent use;
(2), compound is sent into the quick-fried pot of vapour, the quick-fried pot door of sealing vapour;
(3), in the quick-fried pot of vapour, add high steam, and discharge cold air, make the interior air pressure of the quick-fried pot of vapour rise to 1-2Mpa, temperature rises to 140-200 ℃, pressurize 4-6min;
(4), the compound in the quick-fried pot of abrupt release vapour; It is quick-fried to make compound in the process of abrupt release, produce vapour; After vapour is quick-fried compound is sent into airtight fermentation cabin rapidly, adopt aseptic cold water formula plane heat sink and aseptic cold wind to dispel the heat simultaneously earlier, make material temperature reduce to 30-37 ℃; Bacterial classification is inserted in the back, and bacterial classification access amount is 3-5% by weight percentage;
(5), the compound that adds bacterial classification was fermented in fermentation cabin 72 hours, temperature remains on 32-37 ℃; When the temperature of fermentation cabin surpasses 40 ℃, carry out anti-storehouse, or change material over to another fermentation cabin fermentation;
(6), accomplish 72 hours fermentation after, deliver from godown, pack makes finished product.
The particle diameter of each material of the present invention is not more than 5mm.
Among the preparation method according to the invention; The cold water formula plane heat sink that adopts, its frame mode is: the bottom of heat sink is a concave shape groove, and movable cold water is arranged in the groove; Groove face is provided with the flat panel that can hold material; When material is put in when dispelling the heat on the heat sink, dispel the heat with cold water in the bottom, dispels the heat with cold wind in top.
The invention has the beneficial effects as follows:
Adopt the method for gas-explosive material, can make the complete detoxification of material, handle through microbial fermentation, its feed of producing contains amounts of protein isoreactivity material; Not only can replace existing feed, also have short biological growth simultaneously, disease-resistant function is strong; This feed material can make and hold the product raising the output more than 20%, and feed itself is nontoxic, does not have problem of environment pollution caused; Can suppress and overcome with cancer and inferior health phenomenon be the increase of " food origin disease " of representative, reduces the problem that the food medicament residue exceeds standard, the quality better of product; Quality is high, and cost is low, is prone to suitability for industrialized production; For the developing direction of China's employing beans cake manufacture order cell protein feed,, build up people's health and fundamentally solved a great technical problem and social concern for the development livestock economy.
The present invention utilizes the quick-fried technology of vapour can crack protein, produces the oligopeptides material, can make the assorted dregs of rice detoxification of part of interpolation again, improves its utilization rate.In multi-strain fermentation, through aerobic and anaerobic fermentation, microorganism is bred in a large number, its result has not only improved the protein level of fermentation raw material, and Partial Protein is converted into mycoprotein when fermentation.In fermentation, the degradation product of protein comprises polypeptide, oligopeptides, amino acid etc., has globality, comprehensive and concertedness simultaneously.The active absorption function of particularly little peptide; Have than amino acid sooner, higher speed and efficient, and do not receive amino acid whose the influence, product of the present invention both can replace the protein feed of high-quality; Feed addictives such as little peptide, multienzyme and probio have been avoided repeating to add again; Not only reduced aquaculture cost, can increase the yield and quality of meat, eggs and milk again, can make the quality of meat, eggs and milk reach green food standard.
The specific embodiment
A kind of protein feed that is rich in little peptide and multi-enzyme bacteria adopts dregs of beans, cotton dregs, the dish dregs of rice, bone meal, wheat bran, salt, precipitated calcium carbonate, water and bacterial classification, after the fermentation cabin fermentation, processes; The percentage by weight that dregs of beans, cotton dregs, the dish dregs of rice, bone meal, wheat bran, salt, precipitated calcium carbonate and water add is: dregs of beans 50-60%, cotton dregs 10-15%, dish dregs of rice 10-15%; Bone meal 5-8%; Wheat bran 10-15%, salt 0.15%, precipitated calcium carbonate 0.7%; Water 20-30%, the percentage by weight that bacterial classification adds is: the 3-5% of the gross weight of dregs of beans, cotton dregs, the dish dregs of rice, bone meal, wheat bran, salt, precipitated calcium carbonate and water.
The bacterial classification that the present invention adopted; Be made up of bacillus subtilis, bafillus natto, bacillus licheniformis, brewer's yeast, product Ruan Candida, Lactobacillus plantarum and bifidobacterium bifidum, the percentage by weight that each bacterial classification adds is: bacillus subtilis 10-15%, bafillus natto 10-15%, bacillus licheniformis 10-15%, brewer's yeast 10-15%, product Ruan Candida 15-20%, Lactobacillus plantarum 10-15% and bifidobacterium bifidum 10-15%.
Above-mentioned bacterial classification is cultivated through test tube cultivation, shaking table cultivation, first class seed pot respectively, carried out deep-layer liquid by 3-5% access secondary seed jar and cultivate, cultivation temperature 28-37 ℃, incubation time is 24-72h; Viable count is greater than 10
9Individual/milliliter.
The present invention, the seed culture medium of said seeding tank is: brown sugar 5-8%, dregs of beans 1-2%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.15%, precipitated calcium carbonate 1%, surplus is a water, and initial p H value is transferred to 7.0.
The bacterial classification that the present invention adopts all is known bacterial classification, can introduce through Chinese common micro-organisms culture presevation administrative center or the Chinese Academy of Agricultural Sciences microorganism fungus kind preservation center, is applied in the industrialization through screening.
The present invention, a kind of preparation method who is rich in the protein feed of little peptide and multi-enzyme bacteria, carry out according to following method step:
(1), by weight percentage get dregs of beans 50-60%, cotton dregs 10-15%, dish dregs of rice 10-15%, bone meal 5-8%, wheat bran 10-15%, salt 0.15% and precipitated calcium carbonate 0.7%, mix, process compound, after add the water of 20-30% by weight percentage; Make the moisture of compound reach 25%, subsequent use;
(2), compound is sent into the quick-fried pot of vapour, the quick-fried pot door of sealing vapour;
(3), in the quick-fried pot of vapour, add high steam, and discharge cold air, make the interior air pressure of the quick-fried pot of vapour rise to 1-2Mpa, temperature rises to 140-200 ℃, pressurize 4-6min;
(4), the compound in the quick-fried pot of abrupt release vapour; It is quick-fried to make compound in the process of abrupt release, produce vapour; After vapour is quick-fried compound is sent into airtight fermentation cabin rapidly, adopt aseptic cold water formula plane heat sink and aseptic cold wind to dispel the heat simultaneously earlier, make material temperature reduce to 30-37 ℃; Bacterial classification is inserted in the back, and bacterial classification access amount is 3-5% by weight percentage;
(5), the compound that adds bacterial classification was fermented in fermentation cabin 72 hours, temperature remains on 32-37 ℃; When the temperature of fermentation cabin surpasses 40 ℃, carry out anti-storehouse, or change material over to another fermentation cabin fermentation;
(6), accomplish 72 hours fermentation after, deliver from godown, pack makes finished product.
The particle diameter of each material of the present invention is not more than 5mm.
Among the preparation method according to the invention; The cold water formula plane heat sink that adopts, its frame mode is: the bottom of heat sink is a concave shape groove, and movable cold water is arranged in the groove; Groove face is provided with the flat panel that can hold material; When material is put in when dispelling the heat on the heat sink, dispel the heat with cold water in the bottom, dispels the heat with cold wind in top.
Embodiment 1
Get by weight percentage dregs of beans 60%, cotton dregs 10%, dish dregs of rice l0%, bone meal 9%, wheat bran 10%, potassium dihydrogen phosphate 0.15%, salt 0.15%; Precipitated calcium carbonate 0.7%; Mix, after add the water of 20-30% by weight percentage, make the moisture of material reach 25%.
Above-mentioned material is sent into the quick-fried pot of vapour, and in the quick-fried pot of vapour, feed high steam, boost to l.5Mpa behind the discharge cold air, make the temperature in the quick-fried pot of vapour rise to 180 ℃, pressurize 5min.
Raw material in the quick-fried pot of abrupt release vapour, and send into the fermentation cabin of sealing, when temperature is reduced to 37 ℃, can connect the bacterial classification of mixed culture fermentation, inoculum concentration is 5% by weight percentage.
The 72h that in the italic fermentation cabin, ferments, temperature keeps 30-37 ℃, and when temperature in the storehouse surpasses 40 ℃, carry out anti-storehouse, or change another fermentation cabin over to, in whole fermentation process, roll-over 5 times.
Deliver from godown behind the material fermentation 72h, pack makes finished product.
Embodiment 2
Get with dregs of beans 50% cotton dregs 15%, the dish dregs of rice 15%, bone meal 9% by weight percentage; Wheat bran 10%, potassium dihydrogen phosphate 0.15%, salt 0.15%, precipitated calcium carbonate 0.7%; Mix, after add the water of 20-30% by weight percentage, make the moisture of material reach 25%.
Compound is sent into the quick-fried pot of vapour, and in the quick-fried pot of vapour, feed high steam, boost to l.8Mpa behind the discharge cold air, make the temperature in the quick-fried pot of vapour rise to 200 ℃, pressurize 4min.
Material in the quick-fried pot of abrupt release vapour, and send into the fermentation cabin of sealing, when temperature is reduced to 37 ℃, can connect the bacterial classification of mixed culture fermentation, inoculum concentration is 5% by weight percentage.
The 72h that in the italic fermentation cabin, ferments, temperature keeps 30-37 ℃, when temperature in the storehouse surpasses 40 ℃, changes another fermentation cabin over to, in whole fermentation process, roll-over 5 times.
Deliver from godown behind the material fermentation 72h, pack makes finished product.
The result of use of trial employment period:
Try instances 1:2007 March -2008 September, the trial in Xinhua District of Pingdingshan City, Henan Province, HE Zhi Town Village farms, breeding species: pigs; livestock count: 100; 100 pigs reared All edible present invention is made of animal protein feed with fertilizer plus, after the same period by other pigs fed 100 compared to an average weight gain of 9.6%, the survival rate increased by 18.7%, with significant economic benefits.
Try out instance 2:2008 year August in December-2009, try out in Du Shi pig farm, Zhoukou City, Henan Province, all edible animal feeding-stuff containing somatic protein of 150 pigs being raised, average weight gain 12.6%, survival rate improves 36.8%, remarkable in economical benefits.
Claims (1)
1. protein feed that is rich in little peptide and multi-enzyme bacteria; It is characterized in that: after adopting dregs of beans, cotton dregs, the dish dregs of rice, bone meal, wheat bran, salt, precipitated calcium carbonate and water mixed steam quick-fried, process after the fermentation cabin fermentation with bacterial classification, the percentage by weight that dregs of beans, cotton dregs, the dish dregs of rice, bone meal, wheat bran, salt and precipitated calcium carbonate add is: dregs of beans 50-60% again; Cotton dregs 10-15%; Dish dregs of rice 10-15%, bone meal 5-8%, wheat bran 10-15%; Salt 0.15%; Precipitated calcium carbonate 0.7%, the addition of water are the 20-30% of dregs of beans, cotton dregs, the dish dregs of rice, bone meal, wheat bran, salt and precipitated calcium carbonate gross weight, and the percentage by weight that bacterial classification adds is: the 3-5% of the gross weight of dregs of beans, cotton dregs, the dish dregs of rice, bone meal, wheat bran, salt, precipitated calcium carbonate and water;
The bacterial classification that is adopted; Be made up of bacillus subtilis, bafillus natto, bacillus licheniformis, brewer's yeast, product Ruan Candida, Lactobacillus plantarum and bifidobacterium bifidum, the percentage by weight that each bacterial classification adds is: bacillus subtilis 10-15%, bafillus natto 10-15%, bacillus licheniformis 10-15%, brewer's yeast 10-15%, product Ruan Candida 15-20%, Lactobacillus plantarum 10-15% and bifidobacterium bifidum 10-15%;
This protein feed prepares according to following method step:
(1), by weight percentage get dregs of beans 50-60%, cotton dregs 10-15%, dish dregs of rice 10-15%, bone meal 5-8%, wheat bran 10-15%, salt 0.15% and precipitated calcium carbonate 0.7%, mix, process compound, after add the water of 20-30% by weight percentage; Make the moisture of compound reach 25%, subsequent use;
(2), compound is sent into the quick-fried pot of vapour, the quick-fried pot door of sealing vapour;
(3), in the quick-fried pot of vapour, add high steam, and discharge cold air, make the interior air pressure of the quick-fried pot of vapour rise to 1-2Mpa, temperature rises to 140-200 ℃, pressurize 4-6min;
(4), the compound in the quick-fried pot of abrupt release vapour; It is quick-fried to make compound in the process of abrupt release, produce vapour; After vapour is quick-fried compound is sent into airtight fermentation cabin rapidly, adopt aseptic cold water formula plane heat sink and aseptic cold wind to dispel the heat simultaneously earlier, make material temperature reduce to 30-37 ℃; Bacterial classification is inserted in the back, and bacterial classification access amount is 3-5% by weight percentage;
(5), the compound that adds bacterial classification was fermented in fermentation cabin 72 hours, temperature remains on 32-37 ℃; When the temperature of fermentation cabin surpasses 40 ℃, carry out anti-storehouse, or change material over to another fermentation cabin fermentation;
(6), accomplish 72 hours fermentation after, deliver from godown, pack makes finished product.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101032280A (en) * | 2007-04-18 | 2007-09-12 | 上海邦成生物科技有限公司 | Small peptide additive for fodder and preparation process thereof |
| CN101092596A (en) * | 2007-05-23 | 2007-12-26 | 张培举 | Method for producing biological agent of stalk through tiny fluid process by gas explosion under high temperature |
| CN101107970A (en) * | 2007-08-23 | 2008-01-23 | 辽宁省微生物科学研究院 | Bion cow concentrated feed and method for making same |
| CN101401614A (en) * | 2008-10-30 | 2009-04-08 | 河南省龙腾高科实业有限公司 | Multi-bacterial fermentation production of protein feedstuff with steam exploration of vegetables dregs and cotton dregs and method thereof |
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