CN102396644B - Bio-fermented full rate vegetable protein and preparation method thereof - Google Patents
Bio-fermented full rate vegetable protein and preparation method thereof Download PDFInfo
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Abstract
The invention discloses bio-fermented component vegetable protein and a preparation method thereof. Major raw materials are prepared by fermenting with fermentation species; by adopting a bio-fermentation method, the crude fiber content is decreased, the amino acid content is increased and the proportions of various amino acids are balanced while toxic substances and anti-nutritional factors are removed from high-protein cotton dregs, high-protein sunflower seed dregs, rapeseed meal, high-protein safflower dregs and grape pip dregs; the nutritional value of the obtained component vegetable protein reaches and surpasses that of bean dregs; and the component vegetable protein can be used for completely replacing bean dregs in feed application, and particularly has remarkable application effects in aquatic feeds and livestock feeds.
Description
Technical field
The present invention relates to a kind of biofermentation full price vegetable protein and preparation method, belong to the biofermentation field.
Background technology
In recent years, China's animal husbandry and feed industrial development very rapidly, protein is the primary raw material of feed and China's protein raw materials is rare, the protein raw materials aggregate demand is 4,600 ten thousand tons at present, supplies with breach every year more than 2,000 ten thousand tons, needs a large amount of imports.According to calculating-the year two thousand twenty in 2010, China's protein raw materials breach will reach 2,400 ten thousand ton-4,800 ten thousand tons.Dregs of beans is because of its protein content is high, the amino acid quality better shows the major protein raw material as feed, and China's imported soybean was 4,260 ten thousand tons in 2009, accounted for about 45% of volume of world trade, to the interdependency of import above 70%.The fluctuation of the shortage of dregs of beans, external dependence and price has increased aquaculture cost and risk.In the long run; The feedstuff price will rise steadily; High-quality protein such as dregs of beans, fish meal fluctuations in prices of raw materials is frequent, the under-supply fast development that will suppress China's feed industry of protein raw materials, and feedstuff industry will face the predicament that the cost rigidity is soaring, benefit is tending towards descending.
Though domestic more existing plant protein fodder quantity are abundant and cheap, produce 500～6,000,000 tons, the dish dregs of rice per year like cotton dregs and produce 900～1,100 ten thousand tons, the certain herbaceous plants with big flowers dregs of rice 80～1,200,000 ton per year.But because of containing ANFs such as free gossypol in the cotton dregs; Contain ANFs such as phytic acid, tannin, sinapine, sulfur glucosidase in the dish dregs of rice; Contain ANFs such as phytic acid, chlorogenic acid in the certain herbaceous plants with big flowers dregs of rice, cause that its conversion ratio is low, digestibility is low, domestic animal, poultry are off one's feed; Be difficult to digestion and absorption after edible; Should not be used for feed in a large number, must carry out detoxification treatment to improve its nutritive value, in addition cotton dregs, the certain herbaceous plants with big flowers dregs of rice, the dish dregs of rice, the safflower dregs of rice, bottleneck and the difficult point of the grape pip dregs of rice of traditional handicraft production because of being subject to processing technology in order to enlarge its scope of application consumption; Ubiquity heat treatment excessively causes the phenomenon that nutritive value reduces, protein content is on the low side, has also limited its effective utilization in feed.Though and the fermentation cotton dregs, the dish dregs of rice etc. of domestic exploitation recently remove or have reduced the problem of noxious material and ANFs; And effectively promoted total amino acid content; But also crude protein is on the low side in the plant protein fodders such as solution cotton dregs not yet in effect, the dish dregs of rice, the certain herbaceous plants with big flowers dregs of rice, crude fiber content is higher, the unbalanced problem of composition of amino acid, and it still can't substitute the use of dregs of beans comprehensively in feed applications.Domestic in addition less in some essay kind of plant protein feeds such as the safflower dregs of rice, the research and development of the grape pip dregs of rice, its using value and use amount in feed is less.
Summary of the invention
The object of the present invention is to provide a kind of new full price vegetable protein; Solve in common cotton dregs, the dish dregs of rice, the certain herbaceous plants with big flowers dregs of rice that crude protein content is low, noxious material and ANFs content height cause its nutritive value not high; The problem that feed applications is limited in scope, the problem that the composition of amino acid that has solved simultaneously the vegetable protein that general fermentation method makes again is uneven, can't substitute dregs of beans fully.
Technical scheme of the present invention is:
A kind of biofermentation full price vegetable protein is formed through the fermented bacterium fermenting process of preparing by main material, and described main material consists of by weight percentage: the certain herbaceous plants with big flowers benevolence dregs of rice 25～35%; Cotton dregs 30～40%, the dish dregs of rice 20～30%, the safflower benevolence dregs of rice 8～12%; The grape pip dregs of rice 0～8%, the tomato seed dregs of rice 0～5%; Described fermented bacterium: brewer's yeast, lactic acid bacteria, candida tropicalis mix the composite bacteria that obtains after three grades of cultivations;
The protein content of the said certain herbaceous plants with big flowers benevolence dregs of rice is more than 46%, and the protein content of said cotton dregs is more than 50%, and the protein content of the dish dregs of rice is more than 38%, and the protein content of the safflower benevolence dregs of rice is more than 45%, and the protein content of the grape pip dregs of rice is more than 40%;
Each contained in described fermented bacterium bacterial classification weight percentage is: brewer's yeast 40～50%, lactic acid bacteria 20～30%, candida tropicalis 20～30%.
A kind of preparation method of biofermentation full price vegetable protein, carry out according to following steps:
(1) the certain herbaceous plants with big flowers benevolence dregs of rice, protein content cotton dregs, protein content the dish dregs of rice, protein content the safflower benevolence dregs of rice, protein content the grape pip dregs of rice 40% or more 45% or more 38% or more 50% or more of selected protein content more than 46% are primary raw material; Analyze behind its crude fibre, all kinds of amino acid content addition of confirming all kinds of dregs of rice with reference to the ideal protein balanced mode, the percentage by weight of all kinds of dregs of rice components is: the certain herbaceous plants with big flowers benevolence dregs of rice 25～35%, cotton dregs 30～40%, the dish dregs of rice 20～30%, the safflower benevolence dregs of rice 8～12%, the grape pip dregs of rice 0～8%, the tomato seed dregs of rice 0～5%;
(2) all kinds of dregs of rice mix after (1) said ratio batching set by step, get into and pulverize link, and grinding particle size is 40 orders;
(3) it is modified after the pulverizing mixed protein dregs of rice to be added water; Modified back water content is in 45～50% scopes; And insert fermented bacterium liquid with the inoculum concentration of main material gross weight 6～9%; At 28～33 ℃ of temperature bottom fermentations, fermented bacterium is brewer's yeast, lactic acid bacteria, the use after three grades of cultivations mix of three kinds of bacterial classifications of candida tropicalis behind the stirring and evenly mixing, and each bacterial classification weight ratio is brewer's yeast 40～50%, lactic acid bacteria 20～30%, candida tropicalis 20～30%;
(4) during the fermentation fermentate is carried out Analysis of Nutritional, thereby confirm the length of fermentation time, total fermentation time was controlled in 48～72 hours;
(5) fermentation accomplish the back under 90～100 ℃, dry to product moisture less than 10%, and after cooling off pulverization process, promptly get the full price vegetable protein; Products therefrom crude protein>=48%, crude fibre≤7%, moisture≤10%, EAA:NEAA is near 1: 1.1, lysine content 2.3～2.7%, methionine content 1.0～1.2%.
Beneficial effect of the present invention is:
1, with high protein cotton dregs, the high protein certain herbaceous plants with big flowers benevolence dregs of rice, the dish dregs of rice, the high protein safflower dregs of rice, the grape pip dregs of rice method through biofermentation; When removing its noxious material and ANFs; Reduce crude fiber content, improve amino acid content and each amino acid ratio composition of balance; The nutritive value of the full price vegetable protein that makes has met and exceeded dregs of beans, in feed applications, can substitute dregs of beans fully, and particularly result of use is remarkable in aquatic products and bird feed.
2, the full price vegetable protein that makes of this law is when removing noxious material and ANFs; Reach the purpose that reduces crude fiber content, improves amino acid content and each amino acid ratio composition of balance; Its nutritive value has met and exceeded dregs of beans; In feed applications, can substitute dregs of beans fully, particularly result of use is remarkable in aquatic products and bird feed.If 60% in the domestic assorted dregs of rice use this method to produce the full price vegetable protein, year can get the high-grade plant protein fodder about 1,000 ten thousand tons, can alleviate the problem of China's high-quality protein scarcity of resources effectively.
Description of drawings
Fig. 1 is a production procedure sketch map of the present invention.
The specific embodiment
Below the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein only is used for explanation and explains the present invention, and be not used in qualification the present invention.
Embodiment 1
One, raw material and consumption
Main material: the certain herbaceous plants with big flowers benevolence dregs of rice 32%, cotton dregs 30%, the dish dregs of rice 30%, the safflower benevolence dregs of rice 8%;
Fermented bacterium: brewer's yeast (
Saccharomyces cerevisiae), lactic acid bacteria (
Lactobacillus delbrueckii), candida tropicalis (
Candida tropicalis);
Brewer's yeast, lactic acid bacteria, candida tropicalis are all bought in Chinese industrial microorganism fungus kind preservation administrative center;
These three kinds of bacterial classifications are carried out three grades of enlarged culture according to conventional microbe culture mode, mix in proportion, mixed weight percentage is brewer's yeast 40%, lactic acid bacteria 30%, candida tropicalis 30%;
As shown in Figure 1; The certain herbaceous plants with big flowers benevolence dregs of rice, protein content cotton dregs, protein content the dish dregs of rice, protein content the safflower benevolence dregs of rice, protein content the grape pip dregs of rice, protein content the tomato seed dregs of rice 30% or more 30% or more 45% or more 38% or more 50% or more of the at first selected protein content of this method more than 46% are primary raw material, analyze to carry out after its all kinds of nutritional labelings reasonably combinedly, pulverize modifier treatment after the mixing; And after inserting mixed bacteria and carrying out the biofermentation operation; Promptly make the full price vegetable protein, its crude protein>=48%, crude fibre≤7% through operations such as oven dry, cooling, fragmentations; Moisture≤10%; EAA (essential amino acid): NEAA (nonessential amino acid) is near 1:1.1, lysine content 2.3～2.7%, methionine content 1.0～1.2%.
Fermentation accomplish the back under 90～100 ℃, dry to product moisture less than 10%, and after cooling off pulverization process, promptly get the full price vegetable protein.Its crude protein>=48%, crude fibre≤7%, moisture≤10%, EAA:NEAA is near 1:1.1, lysine content 2.3～2.7%, methionine content 1.0～1.2%.
Two, detailed process
1, the certain herbaceous plants with big flowers benevolence dregs of rice, protein content cotton dregs, protein content the dish dregs of rice, protein content the safflower benevolence dregs of rice, protein content the grape pip dregs of rice 40% or more 45% or more 38% or more 50% or more of selected protein content more than 46% are primary raw material, analyze behind its crude fibre, all kinds of amino acid content addition of confirming all kinds of dregs of rice with reference to the ideal protein balanced mode;
2, all kinds of dregs of rice mix after preparing burden in proportion, get into to pulverize link, require grinding particle size all through 40 orders;
3, it is modified after the pulverizing mixed protein dregs of rice to be added water; Modified back water content is in 45～50% scopes; And insert fermented bacterium liquid with 6～9% inoculum concentration; At 28～33 ℃ of temperature bottom fermentations, fermented bacterium is to use after in brewer's yeast, lactic acid bacteria, three kinds of bacterial classifications of candida tropicalis two kinds or three kinds of mixing behind the stirring and evenly mixing;
4, during the fermentation fermentate is carried out Analysis of Nutritional, thereby confirm the length of fermentation time, total fermentation time was controlled in 48～72 hours;
5, fermentation accomplish the back under 90～100 ℃, dry to product moisture less than 10%, and after cooling off pulverization process, promptly get the full price vegetable protein.Its crude protein>=48%, crude fibre≤7%, moisture≤10%, EAA:NEAA is near 1:1.1, lysine content 2.3～2.7%, methionine content 1.0～1.2%.
Embodiment 2
One, raw material and consumption
Main material: the certain herbaceous plants with big flowers benevolence dregs of rice 25%, cotton dregs 40%, the dish dregs of rice 20%, the safflower benevolence dregs of rice 12%, the grape pip dregs of rice 2%, the tomato seed dregs of rice 1%;
Fermented bacterium: brewer's yeast, lactic acid bacteria, candida tropicalis, wherein, brewer's yeast 45%, lactic acid bacteria 25%, candida tropicalis 30%;
All the other are all identical with embodiment 1.
Embodiment 3
One, raw material and consumption
Main material: the certain herbaceous plants with big flowers benevolence dregs of rice 25%, cotton dregs 31%, the dish dregs of rice 21%, the safflower benevolence dregs of rice 10%, the grape pip dregs of rice 8%, the tomato seed dregs of rice 5%;
Fermented bacterium: brewer's yeast, lactic acid bacteria, candida tropicalis, wherein, brewer's yeast 50%, lactic acid bacteria 20%, candida tropicalis 30%;
All the other are all identical with embodiment 1.
What should explain at last is: the above is merely the preferred embodiments of the present invention; Be not limited to the present invention; Although the present invention has been carried out detailed explanation with reference to previous embodiment; For a person skilled in the art, it still can be made amendment to the technical scheme that aforementioned each embodiment put down in writing, and perhaps part technical characterictic wherein is equal to replacement.All within spirit of the present invention and principle, any modification of being done, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (3)
1. a biofermentation full price vegetable protein is characterized in that, form through the fermented bacterium fermenting process of preparing by main material,
Described main material consists of by weight percentage: the certain herbaceous plants with big flowers benevolence dregs of rice 25～35%, cotton dregs 30～40%, the dish dregs of rice 20～30%, the safflower benevolence dregs of rice 8～12%, the grape pip dregs of rice 0～8%, the tomato seed dregs of rice 0～5%;
Described fermented bacterium is: brewer's yeast, lactic acid bacteria, candida tropicalis mix the composite bacteria that obtains after three grades of cultivations; Each contained in described fermented bacterium bacterial classification weight percentage is: brewer's yeast 40～50%, lactic acid bacteria 20～30%, candida tropicalis 20～30%.
2. a kind of biofermentation full price vegetable protein according to claim 1; The protein content that it is characterized in that the described certain herbaceous plants with big flowers benevolence dregs of rice is more than 46%; The protein content of said cotton dregs is more than 50%; The protein content of the dish dregs of rice is more than 38%, and the protein content of the safflower benevolence dregs of rice is more than 45%, and the protein content of the grape pip dregs of rice is more than 40%.
3. the preparation method of a biofermentation full price vegetable protein is characterized in that carrying out according to following steps:
(1) the certain herbaceous plants with big flowers benevolence dregs of rice, protein content cotton dregs, protein content the dish dregs of rice, protein content the safflower benevolence dregs of rice, protein content the grape pip dregs of rice 40% or more 45% or more 38% or more 50% or more of selected protein content more than 46% are primary raw material; Analyze behind its crude fibre, all kinds of amino acid content addition of confirming all kinds of dregs of rice with reference to the ideal protein balanced mode, the percentage by weight of all kinds of dregs of rice components is: the certain herbaceous plants with big flowers benevolence dregs of rice 25～35%, cotton dregs 30～40%, the dish dregs of rice 20～30%, the safflower benevolence dregs of rice 8～12%, the grape pip dregs of rice 0～8%, the tomato seed dregs of rice 0～5%;
(2) all kinds of dregs of rice mix after (1) said ratio batching set by step, get into and pulverize link, and grinding particle size is 40 orders;
(3) it is modified after the pulverizing mixed protein dregs of rice to be added water; Modified back water content is in 45～50% scopes; And insert fermented bacterium liquid with the inoculum concentration of main material gross weight 6～9%; At 28～33 ℃ of temperature bottom fermentations, fermented bacterium is brewer's yeast, lactic acid bacteria, the use after three grades of cultivations mix of three kinds of bacterial classifications of candida tropicalis behind the stirring and evenly mixing, and each bacterial classification weight ratio is brewer's yeast 40～50%, lactic acid bacteria 20～30%, candida tropicalis 20～30%;
(4) during the fermentation fermentate is carried out Analysis of Nutritional, thereby confirm the length of fermentation time, total fermentation time was controlled in 48～72 hours;
(5) fermentation accomplish the back under 90～100 ℃, dry to product moisture less than 10%, and after cooling off pulverization process, promptly get the full price vegetable protein.
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| CN102630858B (en) * | 2012-05-22 | 2013-01-23 | 青岛和美饲料有限公司 | Compound pig feed containing Chinese herbal medicine |
| CN102687796B (en) * | 2012-06-06 | 2013-12-04 | 新疆维吾尔自治区畜牧科学院饲料研究所 | Method for producing bioprotein feed using tomato pomace |
| CN102754765A (en) * | 2012-07-05 | 2012-10-31 | 东北林业大学 | Process for producing peony seed nutrition powder |
| CN102919520B (en) * | 2012-11-01 | 2013-11-06 | 北京桑普生物化学技术有限公司 | Soft-shelled turtle feed using mixed vegetable protein to replace partial fish meal and preparation method and use thereof |
| CN103843980A (en) * | 2012-11-28 | 2014-06-11 | 武汉常辉天添生物科技有限公司 | Preparation method of mixed fermentation feed |
| CN103598415B (en) * | 2013-11-08 | 2015-04-01 | 孙萍 | Sunflower seed meal microbial protein feed and preparation method thereof |
| CN104012774B (en) * | 2014-06-24 | 2016-03-02 | 中国水产科学研究院淡水渔业研究中心 | Megalobrama amblycephala expanded pellet diet of a kind of whole plant albumen and preparation method thereof |
| CN104381598B (en) * | 2014-10-21 | 2015-08-05 | 青岛农业大学 | A kind of method of Biological optimization grape pip dregs of rice nutritive value |
| CN104381588B (en) * | 2014-10-21 | 2015-08-05 | 青岛农业大学 | A kind of composite flora for optimizing grape pip dregs of rice nutritive value |
| CN104938787B (en) * | 2015-07-04 | 2018-01-05 | 新疆泰昆集团股份有限公司 | A kind of cotton dregs compound micro-ecological preparation for being applicable aquaculture and preparation and application |
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